LOCUS       DAFBQW010000001       160143 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFBQW010000001 DAFBQW010000000
VERSION     DAFBQW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 160143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 160143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..160143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.001"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            591..1280
                     /locus_tag="LK889_000001"
     CDS             591..1280
                     /locus_tag="LK889_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5094271.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="LK889_000002"
     CDS             1267..2475
                     /locus_tag="LK889_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5094272.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="LK889_000003"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="LK889_000003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK5094273.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LK889_000004"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LK889_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK5094274.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="LK889_000005"
     CDS             complement(6578..7273)
                     /locus_tag="LK889_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBK5094275.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LK889_000006"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LK889_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK5094276.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="LK889_000007"
     CDS             complement(8472..8663)
                     /locus_tag="LK889_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBK5094277.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="LK889_000008"
     CDS             complement(8684..9574)
                     /locus_tag="LK889_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK5094278.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="LK889_000009"
     CDS             complement(9561..10328)
                     /locus_tag="LK889_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5094279.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LK889_000010"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LK889_000010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK5094280.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LK889_000011"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LK889_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK5094281.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LK889_000012"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LK889_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK5094282.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="LK889_000013"
     CDS             14899..15282
                     /locus_tag="LK889_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094283.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="LK889_000014"
     CDS             complement(15450..16208)
                     /locus_tag="LK889_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBK5094284.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="LK889_000015"
     CDS             complement(16226..17044)
                     /locus_tag="LK889_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK5094285.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LK889_000016"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LK889_000016"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK5094286.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LK889_000017"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LK889_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK5094287.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="LK889_000018"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="LK889_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK5094288.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="LK889_000019"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="LK889_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK5094289.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="LK889_000020"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="LK889_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBK5094290.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="LK889_000021"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="LK889_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK5094291.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="LK889_000022"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="LK889_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK5094292.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="LK889_000023"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="LK889_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBK5094293.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="LK889_000024"
     CDS             27086..27385
                     /locus_tag="LK889_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK5094294.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="LK889_000025"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="LK889_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5094295.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="LK889_000026"
     CDS             27973..28209
                     /locus_tag="LK889_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK5094296.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="LK889_000027"
     CDS             28352..30325
                     /locus_tag="LK889_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBK5094297.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="LK889_000028"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="LK889_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK5094298.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="LK889_000029"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="LK889_000029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK5094299.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="LK889_000030"
     CDS             33204..34496
                     /locus_tag="LK889_000030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK5094300.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="LK889_000031"
     CDS             35232..36095
                     /locus_tag="LK889_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5094301.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="LK889_000032"
     CDS             complement(36224..37846)
                     /locus_tag="LK889_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5094302.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="LK889_000033"
     CDS             38095..38616
                     /locus_tag="LK889_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK5094303.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="LK889_000034"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="LK889_000034"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBK5094304.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="LK889_000035"
     CDS             40697..41515
                     /locus_tag="LK889_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBK5094305.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="LK889_000036"
     CDS             complement(41618..41815)
                     /locus_tag="LK889_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094306.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="LK889_000037"
     CDS             41984..42562
                     /locus_tag="LK889_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK5094307.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42657..42794
                     /locus_tag="LK889_000038"
     CDS             42657..42794
                     /locus_tag="LK889_000038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094308.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43607..44110)
                     /locus_tag="LK889_000039"
     CDS             complement(43607..44110)
                     /locus_tag="LK889_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5094309.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="LK889_000040"
     CDS             44469..45494
                     /locus_tag="LK889_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5094310.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45841..46719
                     /locus_tag="LK889_000041"
     CDS             45841..46719
                     /locus_tag="LK889_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBK5094311.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47195..47311
                     /locus_tag="LK889_000042"
     CDS             47195..47311
                     /locus_tag="LK889_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK5094312.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48239..48577
                     /locus_tag="LK889_000043"
     CDS             48239..48577
                     /locus_tag="LK889_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094313.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48671..48901
                     /locus_tag="LK889_000044"
     CDS             48671..48901
                     /locus_tag="LK889_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094314.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49301..49672
                     /locus_tag="LK889_000045"
     CDS             49301..49672
                     /locus_tag="LK889_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBK5094315.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49946..50638)
                     /locus_tag="LK889_000046"
     CDS             complement(49946..50638)
                     /locus_tag="LK889_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5094316.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50641..51426)
                     /locus_tag="LK889_000047"
     CDS             complement(50641..51426)
                     /locus_tag="LK889_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5094317.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51663..53063
                     /gene="lacG"
                     /locus_tag="LK889_000048"
     CDS             51663..53063
                     /gene="lacG"
                     /locus_tag="LK889_000048"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK5094318.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53088..53405
                     /locus_tag="LK889_000049"
     CDS             53088..53405
                     /locus_tag="LK889_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5094319.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53418..55091
                     /locus_tag="LK889_000050"
     CDS             53418..55091
                     /locus_tag="LK889_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBK5094320.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55105..56736
                     /locus_tag="LK889_000051"
     CDS             55105..56736
                     /locus_tag="LK889_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBK5094321.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57608..58798
                     /locus_tag="LK889_000052"
     CDS             57608..58798
                     /locus_tag="LK889_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBK5094322.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58932..61121
                     /locus_tag="LK889_000053"
     CDS             58932..61121
                     /locus_tag="LK889_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5094323.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61118..62911
                     /locus_tag="LK889_000054"
     CDS             61118..62911
                     /locus_tag="LK889_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK5094324.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            63111..63425
                     /locus_tag="LK889_000055"
     CDS             63111..63425
                     /locus_tag="LK889_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5094325.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            63446..63823
                     /locus_tag="LK889_000056"
     CDS             63446..63823
                     /locus_tag="LK889_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5094326.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            63833..64606
                     /locus_tag="LK889_000057"
     CDS             63833..64606
                     /locus_tag="LK889_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094327.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            64628..66031
                     /locus_tag="LK889_000058"
     CDS             64628..66031
                     /locus_tag="LK889_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK5094328.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            66213..67397
                     /locus_tag="LK889_000059"
     CDS             66213..67397
                     /locus_tag="LK889_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5094329.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            67394..67684
                     /locus_tag="LK889_000060"
     CDS             67394..67684
                     /locus_tag="LK889_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094330.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            67681..67902
                     /locus_tag="LK889_000061"
     CDS             67681..67902
                     /locus_tag="LK889_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094331.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            67944..68723
                     /locus_tag="LK889_000062"
     CDS             67944..68723
                     /locus_tag="LK889_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBK5094332.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            68790..69284
                     /locus_tag="LK889_000063"
     CDS             68790..69284
                     /locus_tag="LK889_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK5094333.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            69340..69978
                     /locus_tag="LK889_000064"
     CDS             69340..69978
                     /locus_tag="LK889_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094334.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            70050..70445
                     /locus_tag="LK889_000065"
     CDS             70050..70445
                     /locus_tag="LK889_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5094335.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            70429..72882
                     /locus_tag="LK889_000066"
     CDS             70429..72882
                     /locus_tag="LK889_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5094336.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            72879..74906
                     /locus_tag="LK889_000067"
     CDS             72879..74906
                     /locus_tag="LK889_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094337.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            74903..75925
                     /locus_tag="LK889_000068"
     CDS             74903..75925
                     /locus_tag="LK889_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK5094338.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            75942..76871
                     /locus_tag="LK889_000069"
     CDS             75942..76871
                     /locus_tag="LK889_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5094339.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            77116..77232
                     /locus_tag="LK889_000070"
     CDS             77116..77232
                     /locus_tag="LK889_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK5094340.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            77248..79167
                     /gene="tet(M)"
                     /locus_tag="LK889_000071"
     CDS             77248..79167
                     /gene="tet(M)"
                     /locus_tag="LK889_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK5094341.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            79265..79453
                     /locus_tag="LK889_000072"
     CDS             79265..79453
                     /locus_tag="LK889_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK5094342.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(79511..79864)
                     /locus_tag="LK889_000073"
     CDS             complement(79511..79864)
                     /locus_tag="LK889_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094343.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            80395..80817
                     /locus_tag="LK889_000074"
     CDS             80395..80817
                     /locus_tag="LK889_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5094344.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            80814..81044
                     /locus_tag="LK889_000075"
     CDS             80814..81044
                     /locus_tag="LK889_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094345.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            81542..81742
                     /locus_tag="LK889_000076"
     CDS             81542..81742
                     /locus_tag="LK889_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK5094346.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            81769..82962
                     /locus_tag="LK889_000077"
     CDS             81769..82962
                     /locus_tag="LK889_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5094347.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(83030..84595)
                     /gene="guaA"
                     /locus_tag="LK889_000078"
     CDS             complement(83030..84595)
                     /gene="guaA"
                     /locus_tag="LK889_000078"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK5094348.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(84654..85577)
                     /locus_tag="LK889_000079"
     CDS             complement(84654..85577)
                     /locus_tag="LK889_000079"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK5094349.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            85773..86378
                     /locus_tag="LK889_000080"
     CDS             85773..86378
                     /locus_tag="LK889_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5094350.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            86686..87702
                     /locus_tag="LK889_000081"
     CDS             86686..87702
                     /locus_tag="LK889_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK5094351.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            87843..89144
                     /locus_tag="LK889_000082"
     CDS             87843..89144
                     /locus_tag="LK889_000082"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK5094352.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            89190..89852
                     /gene="deoC"
                     /locus_tag="LK889_000083"
     CDS             89190..89852
                     /gene="deoC"
                     /locus_tag="LK889_000083"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK5094353.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            89873..90271
                     /locus_tag="LK889_000084"
     CDS             89873..90271
                     /locus_tag="LK889_000084"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK5094354.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            90337..91419
                     /locus_tag="LK889_000085"
     CDS             90337..91419
                     /locus_tag="LK889_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5094355.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            91631..93193
                     /locus_tag="LK889_000086"
     CDS             91631..93193
                     /locus_tag="LK889_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094356.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            93186..94319
                     /locus_tag="LK889_000087"
     CDS             93186..94319
                     /locus_tag="LK889_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094357.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            94319..95275
                     /locus_tag="LK889_000088"
     CDS             94319..95275
                     /locus_tag="LK889_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094358.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            95374..96540
                     /gene="deoB"
                     /locus_tag="LK889_000089"
     CDS             95374..96540
                     /gene="deoB"
                     /locus_tag="LK889_000089"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK5094359.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            96564..97382
                     /locus_tag="LK889_000090"
     CDS             96564..97382
                     /locus_tag="LK889_000090"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK5094360.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            97399..98106
                     /gene="deoD"
                     /locus_tag="LK889_000091"
     CDS             97399..98106
                     /gene="deoD"
                     /locus_tag="LK889_000091"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK5094361.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(98404..99090)
                     /gene="gpmA"
                     /locus_tag="LK889_000092"
     CDS             complement(98404..99090)
                     /gene="gpmA"
                     /locus_tag="LK889_000092"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK5094362.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(99262..99843)
                     /locus_tag="LK889_000093"
     CDS             complement(99262..99843)
                     /locus_tag="LK889_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5094363.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(99976..100659)
                     /gene="rpiA"
                     /locus_tag="LK889_000094"
     CDS             complement(99976..100659)
                     /gene="rpiA"
                     /locus_tag="LK889_000094"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK5094364.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            100978..101391
                     /gene="rpsL"
                     /locus_tag="LK889_000095"
     CDS             100978..101391
                     /gene="rpsL"
                     /locus_tag="LK889_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK5094365.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            101475..101945
                     /gene="rpsG"
                     /locus_tag="LK889_000096"
     CDS             101475..101945
                     /gene="rpsG"
                     /locus_tag="LK889_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK5094366.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            102039..104126
                     /gene="fusA"
                     /locus_tag="LK889_000097"
     CDS             102039..104126
                     /gene="fusA"
                     /locus_tag="LK889_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK5094367.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            104298..105485
                     /gene="tuf"
                     /locus_tag="LK889_000098"
     CDS             104298..105485
                     /gene="tuf"
                     /locus_tag="LK889_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK5094368.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            105892..106200
                     /gene="rpsJ"
                     /locus_tag="LK889_000099"
     CDS             105892..106200
                     /gene="rpsJ"
                     /locus_tag="LK889_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK5094369.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            106231..106860
                     /gene="rplC"
                     /locus_tag="LK889_000100"
     CDS             106231..106860
                     /gene="rplC"
                     /locus_tag="LK889_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK5094370.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            106888..107511
                     /gene="rplD"
                     /locus_tag="LK889_000101"
     CDS             106888..107511
                     /gene="rplD"
                     /locus_tag="LK889_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK5094371.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            107511..107801
                     /gene="rplW"
                     /locus_tag="LK889_000102"
     CDS             107511..107801
                     /gene="rplW"
                     /locus_tag="LK889_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK5094372.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            107840..108673
                     /gene="rplB"
                     /locus_tag="LK889_000103"
     CDS             107840..108673
                     /gene="rplB"
                     /locus_tag="LK889_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK5094373.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            108716..108994
                     /gene="rpsS"
                     /locus_tag="LK889_000104"
     CDS             108716..108994
                     /gene="rpsS"
                     /locus_tag="LK889_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK5094374.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            109017..109364
                     /gene="rplV"
                     /locus_tag="LK889_000105"
     CDS             109017..109364
                     /gene="rplV"
                     /locus_tag="LK889_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK5094375.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            109378..110034
                     /gene="rpsC"
                     /locus_tag="LK889_000106"
     CDS             109378..110034
                     /gene="rpsC"
                     /locus_tag="LK889_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK5094376.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            110037..110471
                     /gene="rplP"
                     /locus_tag="LK889_000107"
     CDS             110037..110471
                     /gene="rplP"
                     /locus_tag="LK889_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK5094377.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            110461..110649
                     /gene="rpmC"
                     /locus_tag="LK889_000108"
     CDS             110461..110649
                     /gene="rpmC"
                     /locus_tag="LK889_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK5094378.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            110674..110940
                     /gene="rpsQ"
                     /locus_tag="LK889_000109"
     CDS             110674..110940
                     /gene="rpsQ"
                     /locus_tag="LK889_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK5094379.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            110998..111366
                     /gene="rplN"
                     /locus_tag="LK889_000110"
     CDS             110998..111366
                     /gene="rplN"
                     /locus_tag="LK889_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK5094380.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            111403..111711
                     /gene="rplX"
                     /locus_tag="LK889_000111"
     CDS             111403..111711
                     /gene="rplX"
                     /locus_tag="LK889_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK5094381.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            111739..112278
                     /gene="rplE"
                     /locus_tag="LK889_000112"
     CDS             111739..112278
                     /gene="rplE"
                     /locus_tag="LK889_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK5094382.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            112297..112482
                     /locus_tag="LK889_000113"
     CDS             112297..112482
                     /locus_tag="LK889_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBK5094383.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            112518..112916
                     /gene="rpsH"
                     /locus_tag="LK889_000114"
     CDS             112518..112916
                     /gene="rpsH"
                     /locus_tag="LK889_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK5094384.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            112948..113484
                     /gene="rplF"
                     /locus_tag="LK889_000115"
     CDS             112948..113484
                     /gene="rplF"
                     /locus_tag="LK889_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK5094385.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            113645..114001
                     /gene="rplR"
                     /locus_tag="LK889_000116"
     CDS             113645..114001
                     /gene="rplR"
                     /locus_tag="LK889_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK5094386.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            114022..114522
                     /gene="rpsE"
                     /locus_tag="LK889_000117"
     CDS             114022..114522
                     /gene="rpsE"
                     /locus_tag="LK889_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK5094387.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            114537..114716
                     /gene="rpmD"
                     /locus_tag="LK889_000118"
     CDS             114537..114716
                     /gene="rpmD"
                     /locus_tag="LK889_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK5094388.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            114760..115200
                     /gene="rplO"
                     /locus_tag="LK889_000119"
     CDS             114760..115200
                     /gene="rplO"
                     /locus_tag="LK889_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK5094389.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            115200..116495
                     /gene="secY"
                     /locus_tag="LK889_000120"
     CDS             115200..116495
                     /gene="secY"
                     /locus_tag="LK889_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK5094390.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            116557..117204
                     /locus_tag="LK889_000121"
     CDS             116557..117204
                     /locus_tag="LK889_000121"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK5094391.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            117395..117613
                     /gene="infA"
                     /locus_tag="LK889_000122"
     CDS             117395..117613
                     /gene="infA"
                     /locus_tag="LK889_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK5094392.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            117646..117762
                     /gene="rpmJ"
                     /locus_tag="LK889_000123"
     CDS             117646..117762
                     /gene="rpmJ"
                     /locus_tag="LK889_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK5094393.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            117780..118145
                     /gene="rpsM"
                     /locus_tag="LK889_000124"
     CDS             117780..118145
                     /gene="rpsM"
                     /locus_tag="LK889_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK5094394.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            118173..118562
                     /gene="rpsK"
                     /locus_tag="LK889_000125"
     CDS             118173..118562
                     /gene="rpsK"
                     /locus_tag="LK889_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK5094395.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            118642..119580
                     /locus_tag="LK889_000126"
     CDS             118642..119580
                     /locus_tag="LK889_000126"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK5094396.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            119608..119988
                     /gene="rplQ"
                     /locus_tag="LK889_000127"
     CDS             119608..119988
                     /gene="rplQ"
                     /locus_tag="LK889_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK5094397.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            120260..121099
                     /locus_tag="LK889_000128"
     CDS             120260..121099
                     /locus_tag="LK889_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5094398.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            121075..121944
                     /locus_tag="LK889_000129"
     CDS             121075..121944
                     /locus_tag="LK889_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5094399.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            121934..122731
                     /locus_tag="LK889_000130"
     CDS             121934..122731
                     /locus_tag="LK889_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK5094400.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            122934..123680
                     /gene="truA"
                     /locus_tag="LK889_000131"
     CDS             122934..123680
                     /gene="truA"
                     /locus_tag="LK889_000131"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK5094401.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(124013..126682)
                     /locus_tag="LK889_000132"
     CDS             complement(124013..126682)
                     /locus_tag="LK889_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBK5094402.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(126920..127108)
                     /locus_tag="LK889_000133"
     CDS             complement(126920..127108)
                     /locus_tag="LK889_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK5094403.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            127378..127740
                     /locus_tag="LK889_000134"
     CDS             127378..127740
                     /locus_tag="LK889_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5094404.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            127792..129474
                     /locus_tag="LK889_000135"
     CDS             127792..129474
                     /locus_tag="LK889_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBK5094405.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            129591..131627
                     /gene="recG"
                     /locus_tag="LK889_000136"
     CDS             129591..131627
                     /gene="recG"
                     /locus_tag="LK889_000136"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK5094406.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            131676..132677
                     /gene="plsX"
                     /locus_tag="LK889_000137"
     CDS             131676..132677
                     /gene="plsX"
                     /locus_tag="LK889_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK5094407.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            132799..133041
                     /gene="acpP"
                     /locus_tag="LK889_000138"
     CDS             132799..133041
                     /gene="acpP"
                     /locus_tag="LK889_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK5094408.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            133309..134313
                     /locus_tag="LK889_000139"
     CDS             133309..134313
                     /locus_tag="LK889_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094409.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            134314..135258
                     /locus_tag="LK889_000140"
     CDS             134314..135258
                     /locus_tag="LK889_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094410.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            135258..136220
                     /locus_tag="LK889_000141"
     CDS             135258..136220
                     /locus_tag="LK889_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094411.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            136247..137161
                     /locus_tag="LK889_000142"
     CDS             136247..137161
                     /locus_tag="LK889_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094412.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            137187..138968
                     /locus_tag="LK889_000143"
     CDS             137187..138968
                     /locus_tag="LK889_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5094413.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            139316..140002
                     /locus_tag="LK889_000144"
     CDS             139316..140002
                     /locus_tag="LK889_000144"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK5094414.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            140022..143603
                     /gene="smc"
                     /locus_tag="LK889_000145"
     CDS             140022..143603
                     /gene="smc"
                     /locus_tag="LK889_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBK5094415.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            143612..144421
                     /locus_tag="LK889_000146"
     CDS             143612..144421
                     /locus_tag="LK889_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5094416.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            144432..145430
                     /gene="ftsY"
                     /locus_tag="LK889_000147"
     CDS             144432..145430
                     /gene="ftsY"
                     /locus_tag="LK889_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK5094417.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            145617..147884
                     /gene="gshAB"
                     /locus_tag="LK889_000148"
     CDS             145617..147884
                     /gene="gshAB"
                     /locus_tag="LK889_000148"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBK5094418.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(147973..148425)
                     /locus_tag="LK889_000149"
     CDS             complement(147973..148425)
                     /locus_tag="LK889_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBK5094419.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            148575..149525
                     /locus_tag="LK889_000150"
     CDS             148575..149525
                     /locus_tag="LK889_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK5094420.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            149518..150477
                     /locus_tag="LK889_000151"
     CDS             149518..150477
                     /locus_tag="LK889_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK5094421.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            150474..151229
                     /locus_tag="LK889_000152"
     CDS             150474..151229
                     /locus_tag="LK889_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5094422.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            151252..152205
                     /locus_tag="LK889_000153"
     CDS             151252..152205
                     /locus_tag="LK889_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5094423.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(152849..153145)
                     /locus_tag="LK889_000154"
     CDS             complement(152849..153145)
                     /locus_tag="LK889_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBK5094424.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            153500..153856
                     /locus_tag="LK889_000155"
     CDS             153500..153856
                     /locus_tag="LK889_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK5094425.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            153866..154765
                     /locus_tag="LK889_000156"
     CDS             153866..154765
                     /locus_tag="LK889_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBK5094426.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            155282..156013
                     /locus_tag="LK889_000157"
     CDS             155282..156013
                     /locus_tag="LK889_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK5094427.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            156158..158185
                     /locus_tag="LK889_000158"
     CDS             156158..158185
                     /locus_tag="LK889_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK5094428.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            158293..159390
                     /locus_tag="LK889_000159"
     CDS             158293..159390
                     /locus_tag="LK889_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK5094429.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            159697..>160143
                     /locus_tag="LK889_000160"
     CDS             159697..>160143
                     /locus_tag="LK889_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094430.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        51702 a        28627 c        32953 g        46861 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttatat
    45661 ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct gagatggtca
    45721 atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa gttttatagt
    45781 tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt catttaaaca
    45841 atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa
    45901 attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat tgtacaagca
    45961 aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga tcctaataaa
    46021 gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt tgcagcatta
    46081 gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt
    46141 ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca tgaacatttc
    46201 caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta cactagtggc
    46261 ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa agggtacacg
    46321 gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga tgcagattta
    46381 tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga actttatttt
    46441 cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc caatcttact
    46501 aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg attattatca
    46561 gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga tgcccaaata
    46621 catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt gcttgcaggc
    46681 gagcacattg gctctggctt aggtattctt acttcataag tttagtactt cgaaatagtt
    46741 taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
    46801 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
    46861 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
    46921 tagtgttcta tcgaacacac agtacgttca ataggtaaac tagctataac aatagatatt
    46981 aaagttaacg gacaagaggg ccttttttag gtagctaaaa cgtaataatg aaaatttatt
    47041 tccagagata ggagtcaatc agctagtctg atcgactgtt ttaacaagtt gactttcatt
    47101 ttaacttttg ataccattta aaacacaaat cgtaattgtt tcgatttgtg ttttttagca
    47161 tgactacaat caaagaagaa agtgggtcgt ccatatggaa aaaacagata tctcaagtgc
    47221 ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga agatcatcaa
    47281 agatgtgaaa cggaatagtg ggaaaaggta agaagaagga aacttaatta aaaaaatagc
    47341 cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg tgtaaggttt ccgaaagaac
    47401 tgctgttgat tttccatttt taacatatga taaattggta gaatctttct tttcaaattg
    47461 ataccattcc ctaattccag ttatatagtt aactacagaa ccacaaagca cgtacgttta
    47521 taaaagtatt atctgtagtt atttttcaat tccctttcaa cctcttattt tgaccagtag
    47581 tattgatcac tttacctttt aacacctttg ttcgtttttg aaacattttt agtcaagaat
    47641 ggaaacgtaa ctacataatc aaaggttttg cgaaatattt catgaaatat ttcatgtaga
    47701 agcgagcaat acggttgcaa ataaccatta tcgtttaata agcagttacg aaagctccca
    47761 atatctctaa cgttcagctc actccatttc ttttcttcaa aaaaatcagt gattcgtatc
    47821 tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat atgcaaaaaa
    47881 ataaaaaagc agttactatt gctagaataa catcaaggaa taataagttt tggttttcag
    47941 ataatagtcg ataagttgtg tattggaatt ttagatacag taataaagta tttttcatga
    48001 aattttgtga gaatcactat tacaagtatc tttaataaaa ttaatggtta aggaggaact
    48061 taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg tgatactaga ctcaaagttg
    48121 caggaataag tttttcaatt tgtgattgga agataaaaaa tgaaaaagaa gggctttttt
    48181 ttatactttg ttgattgtag tgtttatttt ccctctgttt agccaagtaa cattttctat
    48241 gactcaacca gtttcaagtg aggaacatga taaccttttt cgtgctacag aagagtttaa
    48301 gaaatatcag gaaaatattg ttccggagac tccattagct agtgagccaa ttactgatat
    48361 tgatggaaac caaataggat ggtatataca gtacgaagtt atggtggtaa ataatgactc
    48421 aatagtaaaa aatacagtag gcttagagct aacaccgatt gaaaaggtga gttcattgtt
    48481 aacgtatatt tatactgatg aaagtgacga gactgagatc attttaacgg attactcaaa
    48541 aattgctact gataaaaagt atatattgtg gatgtagaca ctagtgaaat actttatgat
    48601 atagaaattg atgaaaattt actagatgta gcacaagaaa tcgatcatca aaagcaggaa
    48661 gctattcgat atgctggtag aaaagggaaa agaaaatatt tattacgtga atgtagcaaa
    48721 agttagagaa gatgaaaatg agtggaaaga attcaagagt cgctactcaa ttaactcgac
    48781 tccgacattt acagtttaca gagaaggatc gatagaaaaa actgtttttt ggactaaaga
    48841 aagtggaatg tctttagctg aagtagaaga gtttttggat tatgttagta tgcaacagta
    48901 agggagttct tattatgaaa aattttgaca catattatgt tattgttttt tcgatatctt
    48961 tgctgttaat attactaact ttaatgcagt ataaagagta tccagagtat tttattttaa
    49021 cttttgtata tttagtaagt agctacacac ttctatataa attcagaagt tcttctagag
    49081 tttctaaagt ttgtttctta attagcttat tgattctata atatttttga tttatatagg
    49141 aaattaaaaa tattatagaa tcaacactca gagaacgggt tggtttttaa tagatttaat
    49201 aagtactgat aatgaaattt ctcctggttg ggaaaacgaa gttggttacc ctgtagaaga
    49261 attttttcaa gattactttg cggagtatgc cgataggata atgagagatt acgtaacaga
    49321 taaaactcaa gaagtcatca atcaatccca gtcattaaat gaattatctg ataaggcgaa
    49381 agaagaagca gttgccatta tttcttcagg gattagtgac tatattgata tgagtggtga
    49441 aatcaattat ggcgataaag acaaaattgt acgcatgata aaagctttta tcaaaacttt
    49501 tgatgtgaag gcagatctta agtttaatga tacttaccta gtaggaaaat taattaatat
    49561 ccttgggaca gagaaagata cggaaacgtt tatttcctct ttgtctatgt tgtttacaga
    49621 taagcatttt tcaactcagc ttggtggaga ggaactagtg gaaatctttt aaccattacg
    49681 cccagcatta ataaaagtat atcaaaaaag tactcctgat gatttttaag attggtttga
    49741 gaaataatgt gaaaacacta tttttaatgt taattataga tagagatgaa cgcgtcatat
    49801 ggttaaaaga atgaaaaagt caaagataca ctgctatatt tcactaagga tatagcagtg
    49861 tatctttcat gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat ttcagttgct
    49921 tttctttggt agttagttga atcgtttaag aattaacgga tgtggcagac aggtgctctg
    49981 tccaaaaacc agtaaaatct ttgatggttc catctttatt tagctgtgcc tgttcaaata
    50041 ctgttttgtt tttaccgaat ggatcaattg caataattgt tccgatcccc gcacgtttgg
    50101 cagcaagtaa tcctgagtaa gcatcttcga taacaagaca atcagccgga gctaagctca
    50161 gtttgtctgc cgctttaaga aaaatatccg gttgcggttt cccagggaaa gagccgtcgt
    50221 catagacaat cttgtcccag tcaaaccagc gggctaaatc gaaaatatca aaataaaaag
    50281 caacattctc tttgacagta gccgtggcaa tcgttaatgg aatctgttgg tcagtcagct
    50341 ggtcaagagt cgcttcaagc ccttttgtca actgaagctc gttttcattt tgcaagcaaa
    50401 gcatacgata atagctttct ttttcttctg acaatacggc gatctcagag gcggtcaaat
    50461 gctctgaaat gaaatgtttc aaaatctccg tattcgtccg accatgaata ttttttaaaa
    50521 tatcctcgtc tgataaacca tcttttgtat gatcatgtat catatgcagc caagcagctt
    50581 catgcagatg agagtccaaa aacattgttc cgttgaaatc aaatataatc cctttcatag
    50641 ttacgctcct atagtggtat tttcggttga atcagcaatt tgcagatcga catattgtgc
    50701 atagtgctct tgaacctccc gcgagatctg gcgatccgta atgagcagat cgaaatcatg
    50761 caaattataa aacacataaa aatcatctcg attgaatttt ttattatcaa cgagtagata
    50821 tttcttacgg gaattattta gagcgatctg ctgtgcttct ccttcttcgg tactgtaagt
    50881 agagatcgct tcgttgtgaa cgccatttga actgataaat gcttttgtaa actttagtct
    50941 tcttaaattg tcgttggtga tcggtccgac aaatgcgccg gtatttttcc ggaaatcacc
    51001 gccaatcagt agaatttctg gtccatctgc ttgagcaaga atatcaaaaa ctggataatt
    51061 atttgtaatg atccgaattt tcttgttcag taaatgcgtc gccaatagtt cgattgttgt
    51121 acctggtcct aagaaaatcg tttctccctc gtgaatcaag gaggctgctg tggcagcaat
    51181 ttcttttttt tcttgaatct gcagagtggc tttttcagtg tgagaaagtt cttgatctaa
    51241 agtgtaggaa agactttgag cccctccatg gacacgcagc agcttgcccg atttttctaa
    51301 ttcatctaaa tctctgcgaa cggtcatatc agaaacatct aattgattga tgatttcatt
    51361 aacggtaagg attccttttt tattgaccat ttctacgata gtaagcaatc tttctttttt
    51421 caacattgtc ttcacctcta atttaaggat aatctatcat ataaacggac aacagtcaat
    51481 ctaattcgaa caaaagtaaa catttagggt ggtttttatg tttgttacaa tagattttat
    51541 tgctgttttc tggtttttat gattatttgt gtttgttttt gttgacaaat gttttggatg
    51601 cgtttacaat gcaatcagtt agaaagagcg aaaactgatt atagatttga ggagagatgt
    51661 acatgcttaa attaccagaa gattttattt ttggcggagc cacagcagcc tatcaggttg
    51721 aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt ttagaggaac
    51781 aagggcgatt cagtcccgat ccggcaagtg atttttatca tcaatatgca aaggacattg
    51841 aattgtgtga acgttttggt gtaaatggtc taagattatc cattgcttgg agccgcattt
    51901 ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc tttttatcat cgtgtgttcg
    51961 aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt gataccccta
    52021 aacgtttgtt tgaccatgga gattttttga atcgagaaac aatagaagca tttgtttcct
    52081 atgccatttt ttgttttcat gaattcaaag aagtcaaggt gtggagtacc tttaatgaaa
    52141 tctatccagt tgcaaccaat caatatttgt taggtgtttt cccaccagga atcaaatatg
    52201 attttacaaa gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc cgtgtagtga
    52261 actatttcaa agaaaacgaa cttccgggtg aaattggtgt agttcactca ttagagacga
    52321 aatatgcggc aacagacgcg ccggaagata agcatgctgc ctttttagac gatgcattgt
    52381 cgatccgttt cttattagat gctacctatt taggctatta ttcaactgaa accttgactg
    52441 ctcttgatga gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa gaagatttcg
    52501 ttgaattgaa aaaagcatcc acgcgtaatg actacttagg gatcaatcac taccaatgcc
    52561 attttgtcaa agcttacgat ggagaaaatg caatccatca taacggtacg ggtgaaaaag
    52621 ggacctctgt ttataaagtg aagggaatcg gagaacgtat ttataaagaa ggaattcctc
    52681 gaactgattg ggactggctg atttatccag aaggtctata tgacttgttg ctgcgaatta
    52741 aaagcgatta cccacattac aacaaaatct atatcactga gaacgggatg gggtataaag
    52801 atcagtttga agatggcatt attatggatc agccgagaat cgactatttg agagtctatt
    52861 tggaatcctt aagcaaggcg ataacagccg gggtaaatgt gaaggggtat tttttgtggt
    52921 cgcttatgga tcttttctca tggacaaatg ggtataacaa aaggtatggc ttgttttatg
    52981 ttgattttga aacccagaag cgttacccaa aagaatcagc ttactggtac aagttggtca
    53041 gtgaaaccaa aacgattatt taataagtga taatggagga agaaaagatg aataaagagg
    53101 aattacaaat gcttggtttt gagatcgttg catattcagg cgacgctcgc agtacattat
    53161 tgaaattatt gaaagaagta agacaaggaa actttgaaca tgttgattcg gcgttgaaag
    53221 aagccgacga aaatttaacc cttgcacata attcgcaaac aaaaatttta gcggaagaag
    53281 catctgggaa agagatggag atgggattca tttttatcca tgggcaggat catttaatga
    53341 ccactttatt gctgagagat ctgattcaag actttattgt actgtacaga cagaatcaag
    53401 gataggagaa gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg aaaccatttt
    53461 ttgaaaaaat ctcacgtaat agttatttag gagccgttcg tgacggattt ttaacagcga
    53521 tgccagctat tttattttca agcattttca ttttgattgc tgcggttcct gagatttttg
    53581 gttaccaatg gcctgaatct gtctccacat ggctatggaa agtctataat tattcgatgg
    53641 gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg cttggctgat tcgatgaatc
    53701 gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc tctgtggtaa
    53761 gtttcctgtt gttaagcgtc tctccgattg aaggtggatt ttcaacggaa tatatgggga
    53821 caaagggact actggcttcc tttgtcgctg catttttaac agttaattta tataagtttt
    53881 gtgtgctgaa agatattacc attaaaatgc ctaaagaagt tcctggaacg atttcgcaaa
    53941 ccttccgaga tatttttcct ttctcttttt cagtatttgc ggccgtttta attgatttag
    54001 gcattcgaca aatcttccaa atctcatttg cagaagcaat cattacggtg ctgcagccgc
    54061 tatttacagc agctgacggg tacttaggaa tcgctattat ctggggagcg atggcgctgt
    54121 tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg gcaattatct
    54181 atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct tctaaagtac
    54241 tgacggtggg tttagggaat tttgtaggaa ctatgggagg aactggggca acattggttg
    54301 ttccttttat cttcctactc tttgctaagt ctaagcagtt gaaggcagtt gggaaagctt
    54361 cctttattcc agtaagtttc gctgtcaacg aaccattgct atttgcggca cccatgattt
    54421 tgaaccctta tttttttatt ccatttttgt taacacctgt tgtaaacgtt tgcctgttca
    54481 agttttttgt cgatgtattg ggcatgaaca gctttatgta cgttttgcct tgggcaactc
    54541 cagcaccgat cggtttggtt ctaggaacgg gcatgggtat cttagctttt gttctagcag
    54601 cggttctaat cgtcgttgac ttcctgatct atctaccatt ttgcaaagca tacgatgctg
    54661 tcttagtaga gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa ggtgcttctg
    54721 ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt tatcagcgat gcaaataaag
    54781 aaatcaatgt gttggtactt tgtgctggcg cagggacaag tgcgatgtta gctaatgctt
    54841 taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt gcttatggtt
    54901 ctcattacga aatgctgcag gattttgata tggtggtctt agcaccgcaa gtcaattctt
    54961 actatgatga tatcaaagaa gacaccgatc gcttaggaat caagctggtt gcgacaaaag
    55021 gagcagaata tatcggatta acgagagatc ctgaaaaagc tgttcagttt attctgaacc
    55081 aatttcaata aaggaggagc cataatgaaa atgggaattt ggttgcgtaa agctgtttta
    55141 tttgcagctg tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc atctgccagc
    55201 gaatttattc gcggagcaga tatttcgatc ctgtcagata tggaaaaatc tggtgcaaaa
    55261 tattatgaag acgatatttc aaaagatgcc ttagagattt taaaaaataa tggtgtcaac
    55321 tatgttcgac tgcgtttgtg gcaggatcct tataatagta atggagaaag ttatggagca
    55381 gggacaaatg atctggaaac gacgattgac cttgcaaaac gagcaaaaaa tctgggactt
    55441 aaggtgctgc tggattttca ctacagtgat ttttgggttg atcctgggaa acagaatctt
    55501 cctaaagcat ggcaaggtct gacctttgaa gaaatgaata cggcattgta cgactataca
    55561 aagaacgttc tgtcggaaat gaagcagctg gacgtttatc ctgatatggt tcagatcggc
    55621 aatgaactga atagcggaat gctctggcct tatggcaagt cttggggaga aggcggggga
    55681 gaatttgatc gattggctgc ttttttgaag tctggtatcc aagctgttcg agacactcaa
    55741 cctaaaacaa ctccagtaat gctccactta gctgatggtg gcgatacagg agcatttact
    55801 tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg atttaattgg agtgtcgtat
    55861 tatccgtatt ggcacggttc aatgaatgaa cttcaaacca atatgaatgc gatcagccaa
    55921 cggtatggta aagaagtgat cgtagttgaa acagcttatg ggcatacaac agcgaatgcc
    55981 gatactatgc caaatgcttt tggtgaagcc gaagcagctg cggggggata tgaaccaaca
    56041 ccgacaggac aagcagagta tttgctggat cttgccgatc ggattcaggc agtaccgaat
    56101 aatcgcgggg cgggcttctt ctattgggaa cctctttggt acaacggaaa tgtgagttgg
    56161 gcaacacaag caggtatgaa ttatctcagt gttcaatcca ctatgggaaa cgaatgggac
    56221 aaccaagcga tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc gtttaaacaa
    56281 gcaggggcgc aaataaatct agttaaaaat gcaagtttcg aaagcgatgg accaacgacc
    56341 agtccttcca attggcaaac ctggtttcaa caaggaaccg ctaagggtac gatcaaaacg
    56401 gaagcgggca ctttttttgg ggactacaaa ctaacttttt ggcaggatca agcatacgag
    56461 gcatcagctt atcaaaatgt tagtggcata agcaaaggaa cttacacatt gagtgcctat
    56521 gtaatgtctg ggggagagtt tgatactaat cagttatata tcaaaaatta tggtggagcg
    56581 gaactaaata ctgcggttgt taaaacaagt gaatggacaa aaattacaat caaagacatt
    56641 ccaattacaa caggtgtttg tgaaattggg gtttatagca aggcttcagg aaatgcttgg
    56701 acgaacgttg attttgtttc attggtgaaa caatagactg cggatcgttt cgattcaaga
    56761 tcggaggttc ccttcagcca catcgttagc gtagaaggca ggaatcgaac ctgcacctcg
    56821 cctttgccaa aaataaccag aaacgatttc cagtcatttt taccaaaacg agtaaagcac
    56881 cagacttcat ctacactttg tattatgcta aagagagttc aacgatttcg taagaagcaa
    56941 tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa gggtaacctt
    57001 agaattccgt cgtgaatatg atttgagcac ttagatggat agaaaaaagt tctcggaaaa
    57061 cagttcgttt ttcgagaact tctttttatg ataagaatga aacaaattaa cttatattat
    57121 agtctctttt ggtagggaac catcttattt ttaatgaaaa tcaagcattt tagctattga
    57181 agaatgttga tttataggga ttccccctcc atattatgtt ggctctttat tcccactctg
    57241 ttttcaacgt aaagtgtata aaacagaatg cgatactatc aagggagtta aaacaatata
    57301 cagaacagta aaacactata aataaaaata aaagtgcaat aaaagtgcaa tttttgcacc
    57361 taatcatact ctgtttatat atgaaaacta gattctgaat ccaatatttg gttaacgaga
    57421 ggcgttcgaa tttgaacgtc ttttatatat tactttcaaa taaatatgag gtagaatact
    57481 atctattttc tttttaaccc aattgttata atggagagtg taaaatattt tgtgtaaata
    57541 gaaaaaagga agtcccttct gtagaataga gttaccacaa cacattcaca gaagagagga
    57601 cttccctatg aacgatttta ctacagaaat tctaaagact ctagcgaaca aaggcgattt
    57661 gaatgaatta ttccgtgtcc atttggagaa agcagtcaat acgcttctca aaacggagtt
    57721 aacggctttc ctagattacg aaaagtatga tcgcattggt tttaacacgg gtaattctcg
    57781 taacggctcc tatgaccgta cggtcaagac cgagtatggg gaacttcatc tccagattcc
    57841 gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct gcttatagac ggacgaatga
    57901 cacgttagag gagaccgtca ttcacctctt ccgaaaaggt attaccatgt cggaaatcgc
    57961 agacttgatt gagaaaatgt atgggcatta ctacacgccc caaaccatgt ccaatataac
    58021 aaaatcattt acagaagagg taacggcgtt taatgggcgg gagcttcatg accgttatgc
    58081 tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg ccaaggaagc
    58141 tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga gctatgcgat
    58201 tgcaccgact gaatccatca cgatttggga ggaaatttta ttggaccttc aagagcgcgg
    58261 tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag gagcgattag
    58321 tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttccc gtaatatcag
    58381 tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat gattttaaaa tggtgtatca
    58441 agcctcatct aaagaggtgg cgttggaagc acgtggtact tttgcggaaa aatggaaaac
    58501 cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca ctttctatga
    58561 tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat cctttaataa
    58621 acaaatcaag aaatacagcc accgcaagga acagttccaa aatgaagagt cgatggaacg
    58681 tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca gtcataaagg
    58741 tttccaacag gccgaaggcg agctcgaaca aatgctaagc caactgattg agaattagaa
    58801 acaatctgca gtagggaaga atcatttaca caaaattatt gacgctccct tataatgaga
    58861 acttttacta acataattta tgataaactt actatataat catattaatt aaatagttag
    58921 gagatctgct tatgtttata tctactcttc aaattcgaaa ttttagaaat tatgaatccg
    58981 aaatctttcg attcgataat gagacaaata ctattatagg tgagaatgat tcagggaaaa
    59041 ccaatgctct tactgcattg agaatcttat tagatgatag ttattactat agtagtaaaa
    59101 cactaaaaga atcagatttt tttcatggaa ttcaaaatgg gtggcaaggg cattggatta
    59161 ttatctctgc gacatttgaa ggaatcagcg aagaagaatt tgacaatgag atttgtgctt
    59221 ccatttcttt aaattctgaa agtcaaacaa tcttagaaga attgatttca aatgcagata
    59281 aaggtgtagg tagtatttct ctatttatta gacctaacaa agctattaga aagcaattat
    59341 ttgatatttc cactgaatta gactctcatc aatttaacga atttagaaat tctattcgtc
    59401 tatccgacta tgaattttac tacacatcta aatcaaatct aaatttctgt atcaatgaaa
    59461 attatgataa attggtagga agactcaatg acaatatagc tagtaatcct gaagaagacg
    59521 atgaagctct gttgggctca agaattgaca tgtcagatat tttcaagcat atttcggttg
    59581 tttatgttga cgctcttcga gatgttctta gagaaatgaa aaacaatcga aacccagtca
    59641 aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga taatgttgag tcattaaaaa
    59701 caattataca gcaattaaat gagactatta ccagtgttcc tgaaattagg aaaattggcg
    59761 aaaatattaa tcggcaacta aatagcataa ttggttcagt gtattctcct aatcttcttc
    59821 tttcctcaac tatgtcagat gatatgggtt ctttagcaaa atttctttca atgaagccag
    59881 aacaaaatat cgacttagac ttactagggt taggacatct aaatatgatt tatttagctt
    59941 tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact acttaatatt atgttgattg
    60001 aagaaccaga agctcatatt catcatcata ttcaaaaaac tctgttcgaa ggattaaatc
    60061 ttcaaaaaaa ctatactcag attctcatga ccacacattc tgtacattta gctgaagtgt
    60121 ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg caaatctatc gttatgaatc
    60181 cagcaaataa tctgaactca tttgctaaag aaaagttaaa aaagaacaat ctaaatctaa
    60241 taaacgcagt agagagatat ttagatgtta aaagaaatgg attgcttttt tctaaaggtg
    60301 ttatcttagt tgaaggagat gctgaagaaa tacttattcc tcaaatagta aaaaaattat
    60361 ttggtataaa cttagacgaa cttggaatag gtttgattaa tgttggtagt acttcgtttg
    60421 aatatatcgc tagcttgttt gacgatgata gaatacagag aaagtgtgct attgtaactg
    60481 atttagatga acaggcaatc ccatcagaac atcgattgta taaaagcact gcacaagaga
    60541 aaggtcaagc aagaaaagaa aaattatcct ccctttttaa tgaaaacaat tgggttaatt
    60601 gtttttatgc taatacaaca tttgaaattg agtttttaga tgtgaataaa gaaaatattc
    60661 taacttatgt cagtccaatt atagatgacg cattcaaaga taaaaaaaca ataactgctt
    60721 ataaagaaga aatacaaagt gaacaatatt tagaaagaaa tttagcgatg ttaaaactag
    60781 ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg tgaaaaaatt gactttttaa
    60841 tcaaaattcc taagtatatt cttaatgcta ttgcttttgc atgtcaggaa atagtagatc
    60901 tctcaattta taaaaaaatt ctactccata caatcgactt gtacgaggaa actacagaat
    60961 tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc tgatttgaat atagcaataa
    61021 tttctttatt agagtacttt ggtaaagatg aggatgttgt gtatctaatc aatcagatag
    61081 aaaattacac aaaaatactt tatgctgagg tttcaaaatg acaaatatct tcgaaaaaat
    61141 cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa agtgaagata acctttatgt
    61201 aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca aggaaaattg cttaccaagc
    61261 aattaaacac gatggatcac taaaaagaat aattgctatc acatacacta atagagctgc
    61321 tgaagaaatt aaggaacgcc taagtttact tagtatagat gatgatatta atatttgggt
    61381 ggggacaata catcagtttt gtcttgaatt tattatttat ccttttaaaa tgaatctctc
    61441 aagagtatca aaaggattta caattattga tgattatact caaagagctt acgtcagcca
    61501 aattaataat atgctgaatt taaatctcaa aacgtatgaa ctgaacaaaa ttgatttgtc
    61561 actttctcat gattttaaaa ttaatgaagt tcagtatact caagtttctg aaatgtatca
    61621 cactatgttg gaaaaaaata aagaaattga ttttgatatg attttactag tagcgtataa
    61681 aattttaact gtaaacagta acgttgcacg taatattgct agtaatataa gggcgattta
    61741 tgtggatgaa tttcaagata ctagagaatt acaatacaat gtaatagcta aattacttca
    61801 aaacaaccct caaattcagt caatgtttgt tggagatatt gaccaagcaa tttatggatc
    61861 attagatggt atagctaaat cagttgaaga actagaaatg ctaactagtc acacttttca
    61921 atcaaagacg ctacatggct gttacaggtc taatcagaga ttagtagact tctactctaa
    61981 ttttcagagt tgtccttacg agattgaaag cagagggaat aataaaaacg accgtggttt
    62041 tataagttat gattgcaaag ttactaagga ggatttaccc aacataataa aaaatataat
    62101 aaaggaaaag attgaaagcg gcattcatga aaaagatatt tgtgtacttg ctcctcaaca
    62161 ctatcctctg ttttcactgg gtgagtattt aaagaaagaa ttaccgtatt gcaattttga
    62221 tgctataaat atatcaccaa ttaaggtaaa taatcatagt ttatttttca agttagcaat
    62281 tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga aagctaattg caaacgatat
    62341 tttaattata ttaaaaaatg aatttggaat agaagttaaa gattatttta ttgacttaga
    62401 tttattacag ctaataaaca gtgtgaaaag aatgtatagt ggagacgaca acggtgttga
    62461 tttatacaaa tttgttacca aacatatatt tgttaatctt aatattgacg agaaaaatta
    62521 cccgtctatt tttaagcact atctattttt tattaacgaa gtagaagatc ggataaaaaa
    62581 taattcatta tctagtagtg tatctagttt taagaagatg tttaaatctc gagatggtat
    62641 cacacttact acagcacata aagttaaagg aaatgagtat gatactgtta tcgctattaa
    62701 tcttctaaac gggaaaattc ctcattggaa tgaaattttt aatgtttctg ataaaggaat
    62761 tagttcagcg agaaaactac tatacgtaat ttgttcaaga gcaaaaaata atttatttct
    62821 tttctcagag gtgggttatt ttacgagtaa aggagacttg ctttatccca ctcaattatt
    62881 agataaaata gaatttgatt ataatattta aaatacctag gtagtcgttg ttttaaatat
    62941 agtaggaatc ggacgctcag tacggagcgt ctattttttt acccaatttt gaaaggaagt
    63001 gaacttatga aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt taaaaccatc
    63061 acacaaataa gattcattca aacataaaaa cagaaaggat aggtaaaaat atggaactta
    63121 aatatgtcat tcccaatatg gaaaaaacat ttggacaatt agaatatgct ggtgaaggga
    63181 acattgaaca acgtcgaata aatggcagac caaccattct ctctcgtagt tacaatttat
    63241 actcaacgat tcaacgagca gatgatattg ttgtcattct tccctccgaa gctggcgaga
    63301 aacattttga atccgacgaa aaagtcaaac tcatcaaccc acatattacc gcagaaggtt
    63361 ataaaattgg caatcgagga tttaccaatt acattatgca cgcagacgat atggtgaaag
    63421 cataagaaag gaaaggtgaa aaaatatgag attagcagaa ggtattgtga ttgaaaaaga
    63481 ccagacattt ggcacattga aattttccgc cttgcgtcgt gaggttcgta tccagaatga
    63541 agatggtacg gtttccgaag aaattaaaga acgtacctat gacttaaaat caaaaggaca
    63601 aggacgcatg atacaagtca gtatccccgc ttctgttcct ttgaaagaat ttgactacaa
    63661 cgcagaagtg gaattaatca atcctgtggc agataccgta gcgacggcta cttttcgagg
    63721 ggcagacgtt gattggtacg tgaaagcaga cgatcttgta ttaaaaggac agacaaaacc
    63781 tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc ttatgacaaa
    63841 cacaccattg attttgaaag aaattccgaa ggatgaagcc atttctttta ttcggcaata
    63901 ccattattca aagatactcc ctagattgtg taaatacttt ctagggattt tttcagaaga
    63961 aaaattatta ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa ccatacgcaa
    64021 actctttccc gacagttccc ttcaaaccac agattattta gaaattggaa agatgtgttt
    64081 tcttccagaa atgaatcaaa ccaattattt tggcagtcaa gctctttcag ctctaattaa
    64141 gtggttgaaa gaacatacgg actgtcattt tttatataca ctggcagatg gaattgaggg
    64201 aaaatgtggc tatgtctatc aagcgtccaa cttcttttac tgcgggtatt tcaagacaag
    64261 tgtctatcgt gataagcagt catgggaaaa gatacaccct cgaagtgctc gccttttatt
    64321 agaggaaaat gctcgctttg aacaggtaga gaaaaagcat tggctttccc aggcattttg
    64381 cgaatacaaa gggattgaga aaatcaatgg acgcatgttt cgctatctct atccgttgac
    64441 aaaagaagcg aaaaaactgt taggacacac tctctatcga cgtcattatt atccgaaaga
    64501 aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca tttctcaacc
    64561 aacttttgat aaacaggctc gtatctacaa tacacaatta ttttgatagg tggaaaggag
    64621 gatttttatg ccattgcttc atgacagagg agagcgaatc aaagccaacg ataagcactt
    64681 ggtctatcat tttgtactaa tttcattgct ggtagttttt ctagtcgtcc tactcccttt
    64741 tcattggaga gcgttacaat cggtgaactg gcaagcagtt cagttagata catttttcta
    64801 tcaacttcat acgccatact tttggagcag tattctcact gctctttttg tatgcggact
    64861 tatcagctgg ctttattatc actatcgtat agattctata aaacagttag agcaccgtca
    64921 aaagttagct cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa ctcaatcaga
    64981 gggattcttt aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc attttcctaa
    65041 aatatattat cgaatgaaag atggactaat ccatattcat gtagaaatca ccttagggaa
    65101 atatcaagat cagcttttgc acctagaaaa caaactagaa acaggattgt attgtgaatt
    65161 agtggaaaaa acgttgaaag attcttatgt agagtatgtc ctactttatg atactattgg
    65221 caatcgaatt ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat tgatggaatc
    65281 ggtctattgg gagttcgatt ctctccctca catgttaatt gctgggggca ctggtagtgg
    65341 aaaatcctat tttattctca cattgattga atctctcctg catacaaatg caaagcttta
    65401 tattcttgac cctaaaaatg ctgatttggc agacttaggg gcggtcatga ataatgtcta
    65461 ttaccgtaaa gaagatatgt tggcatgtat caatcaattt tacgaagata tgattgatcg
    65521 cagtgaaacc atgaaacaac accctaacta taaaacagga gaaaattatg cttatcttgg
    65581 tttgtctgcc aattttctta tttttgatga atatgtggca atgatggata tgctaggtcg
    65641 tgaaagtacc accgttattc ataaactcaa acaaattgta atgttaggac gacaagcagg
    65701 cttcttttta attcttgcct gtcaacgtcc agacgcaaaa tattttagtg atgggattcg
    65761 ggacaacttt aacttccgtg tggctttggg tcgtatgtct gaaatgggct ttggtatgat
    65821 gttcggaagc gatacacaaa aacaattttt cttaaaaccc atcaagggaa gaggttacgt
    65881 ggatacagga aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa aaaggtacga
    65941 tttcttagga gaaataggaa aagtcattca aaaaaagcaa tctgagcctg taccgcacga
    66001 agtgaaaggt acaggttcag acttgctttg attggtgtgg cgttagccac gccaatcctt
    66061 tagccccctc tatctaacag gggggcacaa atacaactga aaacaggcaa ttctcatcaa
    66121 ccaatgttga tggtacaaag gattgcggaa aatatgaaag gtgtaaactc aagggtcata
    66181 gtttacatct tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt tggattaagg
    66241 aattgaaaga aaagcgactg gcttatggcg tttctcaaaa taaactggcg gttgcttctc
    66301 atattaccag accttaccta tcggatattg aaacaggcaa ggcagtccct actcaacagg
    66361 tgaaagaaga tttactgaac gctttggaac gcttcaatcc cgataaccca ttggagatgt
    66421 tattcgacta tgtacggatt cgattcccta ccaatgatgt ggctcaaatt atcggtgagg
    66481 ttttacgttt gaatatggat tatatgctac acgaagactt tggctattat tcttatcccg
    66541 aacactatcg tttcggtgat attgttgtcc tagtctctca tgatgtaagt aaaggtgttt
    66601 tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg gctcaacatc
    66661 ggagctggta tgatttcttt caagattgca tggaacataa aggtattttc aaacgcttgg
    66721 atttagctat caacgataaa acagggattt tgaatattcc agatttggca aagaaatgta
    66781 aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt ggtgaattgg
    66841 ttcaccgtga tgaaaaacca tacatgggaa acacacttta tattggctca ttaaaaagtg
    66901 aagtctattt ctgtatatat gagaaagatt atgagcaata cgtgaaaaat gatgttccct
    66961 tagaagacgc agaagtaaag aatcggtttg aaattcggtt aaaaaatgac cgagccaccc
    67021 aagccatgaa agatttattg gcacatcaac aggcggaaaa aacagccttt gaaattatca
    67081 atcgctatat ccgctttgca gataaagacg acactaaacg tcggagtgat tggaaaacta
    67141 atgaacgttg ggaatggttt atcggtaaaa atcgtggagc attacgccta accacacagc
    67201 cagagcctta ttcttttgaa cgaacattga actggttaca tcatcaagtc gcccctacat
    67261 taaaaatcgc aagcatttta gatgttctca acggtacaac cattatctcc accatgattc
    67321 aggaagccaa actgacagaa aaacatgaga aattaattga acaacaacat ctagccatgg
    67381 aggatttgat tacatgagcc ttttaacaaa agaattaaag aagttagggt ttcagtgtgg
    67441 cattgagttt caagcgtata tacaaaatac agggaaatat acgtctctca ttattgaggg
    67501 aaaacggcaa gctggtgata ccatctatac ctatgatttt tacaaggtga gattttataa
    67561 taattatacc aatcgagtaa ccgtctatgg agagcattta accccttttc aattattaag
    67621 gcgtgtaaaa agctatatct attatcgaga aaaatatctt aaggaaagga ggacaatcac
    67681 atgaattttg ggcagaattt gtacaactgg ttcttggata atgcacagtc attggtattg
    67741 ttagcgattg ttgtcattgg actgttttta ggttttaaac gagaattttc aaaactcatt
    67801 ggcttcttga ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc tggcgtgaaa
    67861 gatgtgttat taaacttatt taaccgaatc attggtgctt aacagatatt tttcatgata
    67921 aaataggaag tatatatttt gttatgaaag gatttatgaa tatgtttaca aatgatcaat
    67981 ttaaatctct tttaattaaa acattagaaa gaaagagctc ttcaaattat tatgaaggag
    68041 gtaacgagat tgtttctaaa atgaaaatat cagaggtaac tttagatgag actgacgatt
    68101 ttacttatta taaaggatat aaaaagggct ggaatacgct ttgcacatat cttaaaataa
    68161 gagttccttt tgatgattta gacttttttg aaagtcataa agatttgatt acccagacag
    68221 caagctccat atatgataaa caaggtgata atgtattagt ggatactatt ttagtaccac
    68281 ttccagagaa ttacgaagta attaacttta gtcagttgaa aattagtgat gtggtttcac
    68341 aagctattga agacgctgag tcttttatga gtaatggtga atatcaacgt gcctttgaca
    68401 gagttcacac tgcttttcat ggatatttaa ttgaaatact aaaaaaatat gaaattacag
    68461 ttcctagaga cgaaaatcta tctaaattat atagtaggat acaacaatta attgaaaaag
    68521 aaattcaacc tactgaactt gctgatatag tgaagacaac gataaggtca tcaaatggaa
    68581 tgattagctc tcttaatgaa gcaagaaata ggcattcact tgcacaccct aatacaaata
    68641 ttattggaaa gagagaagca aaactaataa taggtatttc aagtacagtt acagattata
    68701 ttagcggata ccttgataag tagcgtcatt aactgattgc tacttttttt atgactattt
    68761 ttataaaaag gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat ttaggacggt
    68821 ataatgaagg tgaattagtc ggagcatggt ttactccacc aattgatgag gaagaaatgg
    68881 cagaaagaat cggtttaaat gaggactacg aagaatatgc aatacatgat tttgagcttc
    68941 cttttgatgt ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt gaagccattc
    69001 aagaaatcga aggtacacct atttataatg aactcaaaga aattcaaggc atgtggttta
    69061 gtagcttgga agaattactg gaaaacaaag aggatattca ttgttatagt gattgtgatt
    69121 cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg acagcttggt gaagtccctt
    69181 ccaatttaca aaattatatt gactaccaat ctttgggacg tgatatggaa atagagggta
    69241 attacctagt tacctctcat ggggtatttg aatattgtca gtaagtttaa caatccgtgt
    69301 tataattaaa ttaataaata gcacggagag gtataaatta tggtaaatgt atttgaaaat
    69361 cttaaactat taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt
    69421 cgttacaaac agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt
    69481 ccagaatatg ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta
    69541 gctgtctatg ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt
    69601 ggtatcgaat acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct
    69661 cgatttattc cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt
    69721 ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga
    69781 agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa
    69841 acgagattgt atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc
    69901 agatattcaa tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca
    69961 aaaaataaaa ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt
    70021 ttctattttt acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt
    70081 ggtcagtaga aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct
    70141 ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac
    70201 caccactttc tctgattgac ggagcactac tcaaatactt agggattccc attgttctca
    70261 ctgggttcat gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta
    70321 tcattaccta tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa
    70381 aagaaacaat gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg
    70441 attaaatata tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat
    70501 gaattaattc cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat
    70561 aatttctgtc aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca
    70621 accgaatcaa gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta
    70681 aaagagatag cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt
    70741 gacaatcaag tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa
    70801 gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt
    70861 gtgaatcaca aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt
    70921 tctaagatgg aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg
    70981 gagaaagatg attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt
    71041 gatgactacg actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa
    71101 tacgatattc tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt
    71161 gaacaggaag ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa
    71221 ttggatttcc ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt
    71281 gataccagta tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat
    71341 aaaaagaaag aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt
    71401 actaatgtgg tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc
    71461 aaagaatcta tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag
    71521 ctgaaacaac gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta
    71581 cgaccttttg gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat
    71641 atcaatgact atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct
    71701 acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga
    71761 aatatttatc tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca
    71821 ttagcgtccg cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg
    71881 gtctattatt cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt
    71941 ggtgaatgga aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg
    72001 agtgagaacg acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat
    72061 tcagaaagcc ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc
    72121 gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg
    72181 ttgttattgg tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct
    72241 gaccatatcg aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt
    72301 gtcacccaat ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc
    72361 ttacccgatt ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca
    72421 atgctgattg tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt
    72481 aaaattgttg accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta
    72541 tctatgcgat tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa
    72601 aacgcggacg atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt
    72661 cgttccactg acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa
    72721 gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac
    72781 atctatggtc gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc
    72841 tttgatacca gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat
    72901 ttcttcacta tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca
    72961 cgctgggcaa tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg
    73021 aatactccaa gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg
    73081 gctggcttcc atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg
    73141 ccattactaa ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg
    73201 tgaaagaagc ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca
    73261 tgcagacgat tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt
    73321 tcctccttct ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc
    73381 gagaaacgac gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg
    73441 cttcctttat tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg
    73501 tcagtaccgc cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag
    73561 ggaaagacag tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat
    73621 ggctattgct tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa
    73681 gtttattgtc taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg
    73741 aagaaattga agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag
    73801 gaacagtctt ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa
    73861 caggaatcat gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca
    73921 gtttcctatt aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt
    73981 tatttaatgt gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta
    74041 tttccactat gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac
    74101 aaattgtgac ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt
    74161 tacaaagcaa cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga
    74221 tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag
    74281 ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata
    74341 ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc
    74401 aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa
    74461 atcgtctagt caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg
    74521 tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac
    74581 aatctatggc agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa
    74641 aacgaaagaa cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa
    74701 aagaaaaccc atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa
    74761 ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa
    74821 aagagcaaaa acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa
    74881 cggataagcg aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt
    74941 ctctctcttc ttttttctct tattattcct agtcattttc tttgcagatg atacggacag
    75001 tggcgaaaac aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt
    75061 gttggcacac cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt
    75121 ttcttatatc ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca
    75181 aagttccgaa tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca
    75241 aggggttaaa tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga
    75301 ttctgttatc caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg
    75361 gaaaaaatat acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa
    75421 agcagactat cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg
    75481 aaatcagttt tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt
    75541 tgatggtgaa accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta
    75601 tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt
    75661 gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca
    75721 agaaatctct atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa
    75781 tgcaggcaca tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc
    75841 aggcgaccca atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg
    75901 ggctagacgt gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc
    75961 gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc
    76021 cgatgattaa agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg
    76081 gtagtgtcgg atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg
    76141 aaacagaaat cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat
    76201 cctttgcgaa agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg
    76261 aaaaattgac ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta
    76321 aggatattcc tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga
    76381 atgagaacac ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca
    76441 aggaaactat atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg
    76501 ttattatcaa aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac
    76561 agctagaaag tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag
    76621 aaacgttctt ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta
    76681 atcatgtgtt acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct
    76741 ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa
    76801 caaaggcaac ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga
    76861 ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg tactcttttt
    76921 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtgacta
    76981 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    77041 cgggaacaaa taattagatg tcctttttta ggagggctta gttttttgta cccagtttaa
    77101 gaataccttt atcatgtgat tctaaagtat ccagagaata tctgtatgct ttgtatacct
    77161 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    77221 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    77281 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    77341 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    77401 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    77461 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    77521 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    77581 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    77641 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    77701 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    77761 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    77821 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    77881 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    77941 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    78001 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    78061 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    78121 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    78181 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    78241 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    78301 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    78361 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    78421 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    78481 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    78541 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    78601 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    78661 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
    78721 tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt
    78781 actccagcag attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct
    78841 ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt
    78901 tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa
    78961 aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat
    79021 gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag
    79081 gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta
    79141 agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct
    79201 tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat
    79261 tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg
    79321 aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga
    79381 aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac
    79441 gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga
    79501 attttactga ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca
    79561 ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttcgag
    79621 tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca tggagtaatg
    79681 atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct atatttgtta
    79741 aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga ccccgtttca
    79801 ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct tcttttttac
    79861 gcatactaaa tcacctctat acattttact gttcctatat aaccataaac aggtatagaa
    79921 aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt aatgcgatga
    79981 atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt ataaaattta
    80041 gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag tcgccatgag
    80101 aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac ttatccacct
    80161 gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag aaaaatcaaa
    80221 gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa taaatatctt
    80281 ttaggatttc tcttcattta tgcaacaaat ccccctttcg tgtcggggtt tagtatgaag
    80341 ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa aagcatgaaa
    80401 ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa acaagttatt
    80461 gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa agaagtctcg
    80521 ttttctgata tagataacta ccttgtcaac tctttttcta ctgttgatac gtacgtcact
    80581 gatttccaac tatttaaatt atttggatta agaatcaatg ttgaaagtga tttattaagt
    80641 gaagccctgc gtcatctttc agagaaaaaa cggaacatta ttttacttca ttactttatg
    80701 gatatgagtg atgtggagat tgcggagtta ctaaatttga atcgttcgac tgtttatcgt
    80761 catcgaataa gtggactagc catgattaaa gaattcatga aggagtgtaa agaatgaaaa
    80821 cagcttatcc aagggttccc ttccccctca ttgtaaaggc gacagatggc gacgtggaag
    80881 caattaatca gattgttaaa cattatcgag ggtatacctc aaaacgttca ttacgtcgca
    80941 tgacagacga atatggaaat tctcatatgg ttattgatga aaccctacgt ggcagaatgg
    81001 aaacgaaact catctctaag attttatcgt tcgaaattaa atagtcccat tttttcgtgg
    81061 aagcgtgtcg aatcgtatcc acgcttcccg atttgggagg tttgttattc catcaaagca
    81121 tattgtgtgt tcagtaggtt ttgataggct aacaagccaa acgttctttg acaaccaaat
    81181 aaaggcaatc gaatacgttt cggtaagaaa agagctgatg aactaacacg ccatgaccta
    81241 caaaattagt gagcgattca tgttagtaat tcgaaagtaa tctttagcag gattgctgcc
    81301 atgactttct tatcgtgata atgatactcc cgtatagcca tagtccgagc gttagaagcg
    81361 tcacaggcaa tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc tgtgagcttt
    81421 gctagagctg ttcgattgct aagatattct tctcttgtca gtgtattatt ataaaactat
    81481 ttgatataat tataggtgaa tattggagtt ttcctagcct ataattggga aaggagtaat
    81541 aatgataacg gtcacaaaaa aggatttagt tgagctagga tatggaactt ctttttcagc
    81601 agacatcata aaaaaagcaa aagaattgat gattacaaaa gggcatatct actatcaatc
    81661 tcgaaagcta gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa ttcggttttc
    81721 aaatgaaaac aggtctgagt agtttttgca cagtaaggag taaaaactat ggttaaagac
    81781 ccaataaaaa aagcaaagaa tggaacctat tattttcgtg caaatctagg ttatgattct
    81841 aaagggaaaa aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga agcaagagaa
    81901 gaatattcga aactgctatt gatgaaacca gaagaattaa gtgagaataa ggataaaatg
    81961 acgtttaaac attatatctt tgaaattttc cttccatggt ataagacaca ggtaaagctc
    82021 agaacatatg aaaatcgctt accaactatt aagaagcatt tctcttattt tgataaaatg
    82081 gctgtttccg atattgagcc tattgatgtg caaaattggc aactaaaatt atcaaagaag
    82141 tgtaagtctt cttatgtaag agctgttcaa ggactatttt cagttgctat ggatcgagca
    82201 attgtccttg ggataaccac cacaaatcca tctaagatta ttggcaatgt gaaaaagcaa
    82261 aagtctaaaa ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc tctaatttac
    82321 aaagaagatt actatcaaca ctttttattt atctctctgt ggtttttatt tatgactgga
    82381 atgcgaattg gtgaagctac tgccattcaa tgggaagata ttgattttga ctcaggagta
    82441 ctaaccatca acaaaacact ctattacaag aatcaatcga attatagatt tgttgaacca
    82501 aaaacaaagg cgagcgttcg tcatattgta ttagataaat gcacattaac ttatttatcc
    82561 gaatggaagg aagtacaaca aaatctaatt cagacagact ttgtaatgag ctataacggt
    82621 attcctaccc aaaaacatac actatcatat gctattgaac gctatgctaa gctggctggt
    82681 atccatcgaa tcagaatcca tgggttacgg cattctcatg cttccttgtt aatcagtatg
    82741 ggagaaaatc cacttatcat taaagatcgt ctaggacacg aagatattga gacgacttta
    82801 ggaacgtatg gacatcttta tcctaacagt aatttcgagg tagctaacaa attaaatgga
    82861 gccatttcct ttaaagaagc aaaacaaaat tatgatactt caccaaagaa tcaatttact
    82921 gtagactact tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag taaaaagata
    82981 agaagagcca gaaacccttg gtataagagg atttctagct ctcctacaac tattcccact
    83041 ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt
    83101 cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag
    83161 cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc
    83221 cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat
    83281 cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga
    83341 tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga
    83401 atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt
    83461 cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc
    83521 cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt
    83581 cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg
    83641 cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt
    83701 cagatacacc tttcaacttg tctaagaagc gatctcttgc atcgactttg atgatgttca
    83761 agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac
    83821 cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa
    83881 cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt
    83941 cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt
    84001 gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt
    84061 ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac
    84121 aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat
    84181 ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg
    84241 ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa
    84301 gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga
    84361 agatctcagg atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa
    84421 gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata
    84481 attcggaaaa gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac
    84541 cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta
    84601 ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat
    84661 tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct
    84721 cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt tttccataca
    84781 tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc
    84841 tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg
    84901 atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt
    84961 tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc
    85021 tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact
    85081 tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca
    85141 taactttcag gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga
    85201 taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa
    85261 atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg
    85321 ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa
    85381 gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga
    85441 acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc
    85501 aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag
    85561 ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact attttaacac
    85621 aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta taatctttat
    85681 aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg
    85741 ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc
    85801 cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat
    85861 ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga
    85921 ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg
    85981 ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg
    86041 acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac
    86101 atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta
    86161 tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg
    86221 catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag
    86281 cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag
    86341 ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg
    86401 acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga
    86461 agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt
    86521 gttttttacg ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag
    86581 ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg
    86641 ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc
    86701 agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac
    86761 aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat
    86821 cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga
    86881 atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc
    86941 tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat
    87001 gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca
    87061 ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc
    87121 attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat
    87181 gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg
    87241 ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg
    87301 ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc
    87361 caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt
    87421 tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa
    87481 atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt
    87541 ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc
    87601 ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa
    87661 ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt
    87721 atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta
    87781 aagtggttta cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg
    87841 caatgcgaat ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg
    87901 aaatcgagta catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg
    87961 cattattgat ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat
    88021 tggcgattgc taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag
    88081 ataagcattc gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag
    88141 ctagtgtcgg agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta
    88201 cactagacaa gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg
    88261 tacgcattgt taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg
    88321 cggacaaaaa attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga
    88381 ttgccagttc gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg
    88441 tgaccactgg agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga
    88501 ccatgacgag cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt
    88561 cagaacctct tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat
    88621 tacaaggaaa tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg
    88681 ttgttcttgc tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc
    88741 tggaatcagg taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc
    88801 cagcgatcgt ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg
    88861 ctaaacaaag cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga
    88921 tgctaggtgc tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac
    88981 tgcataaaaa aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta
    89041 acgacgaaga aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat
    89101 cggcaatgaa gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact
    89161 ttaccgtttt aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta
    89221 ttttgaaagc agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat
    89281 accattttta ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac
    89341 aaggtgagcc agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg
    89401 agacaaaagc atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg
    89461 tcatcaatat cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag
    89521 cagttgttga agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat
    89581 tagatagaga agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg
    89641 tcaaaacatc aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc
    89701 gtgaaactgt aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag
    89761 aagcaatggc aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa
    89821 tcgtatctgg ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga
    89881 actcaaacaa gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta
    89941 ttcccatttt ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat
    90001 caacattgag aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa
    90061 agcagtatct gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga
    90121 tcctatctct ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat
    90181 gccggttacg ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct
    90241 gccttatgca tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg
    90301 aaaaataaaa caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt
    90361 ttaggtgctg tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct
    90421 actgattcat ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac
    90481 acaggtggtg tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg
    90541 gggaaagatc aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat
    90601 gaatcagatt acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt
    90661 ggtattggtt ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg
    90721 aactttgtta ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc
    90781 aaagacaacg aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac
    90841 gtagtcggct ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc
    90901 aaagccggtg ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa
    90961 tatgctggtg acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca
    91021 caaaacgcag atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa
    91081 gcaaaatcat tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac
    91141 caatcagacg aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct
    91201 acactgaaag cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa
    91261 ttccctggcg gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa
    91321 ggacaacttt ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca
    91381 ggtgacgtaa aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt
    91441 agacacatgt ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc
    91501 tgccttgtga cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa
    91561 gaacgctcac taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg
    91621 tgaaaaaaag gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt
    91681 tggaacattc aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc
    91741 gttgcttgga gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt
    91801 agagccgaca tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac
    91861 caaagccaac caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt
    91921 tactgtaaca gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca
    91981 taaaaaagca cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc
    92041 agatgcttac gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac
    92101 actttaccga ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca
    92161 agaaattgat gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat
    92221 tttgattacg cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg
    92281 ccgtggaaaa ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga
    92341 tatgatggtt ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga
    92401 agtcgttttg tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa
    92461 aaacttgaac ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa
    92521 cgggcagtcg gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat
    92581 caagctaaaa ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt
    92641 aggacacgtg ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga
    92701 aaatatcgca ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta
    92761 ttcagtaatg aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa
    92821 tgaacttgtt cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg
    92881 tgaagtcgac cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt
    92941 aggtgcgatc gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt
    93001 gttactgatc agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat
    93061 ccatgaagga gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg
    93121 tctattgatg gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg
    93181 cgcacatgaa tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg
    93241 ttttaggatt gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag
    93301 gttattcttc tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa
    93361 ggcaagcaac gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt
    93421 ttttcaatat tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg
    93481 cacttgcgtt tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg
    93541 ccttagctgg tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa
    93601 gtgaagtgat cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt
    93661 tacatgaagt gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc
    93721 aaaatgcctc actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact
    93781 caggattatt tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga
    93841 ctttaggatt tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa
    93901 tgagtagtaa acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg
    93961 gcggtgtggt tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat
    94021 ctattggatt tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc
    94081 ttttatcagc gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag
    94141 caggtgttcc gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga
    94201 tcaacttcct gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga
    94261 tcgttgcgac aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat
    94321 gtcgactatc gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct
    94381 aattctaacg tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct
    94441 agagggaatc atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc
    94501 atcacttggt tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct
    94561 attttctctg attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg
    94621 gacagtaatt aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg
    94681 taaaggtcaa actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc
    94741 agatattccg gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc
    94801 tattttgatt gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct
    94861 ccgttcagtt ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat
    94921 gcgttattca ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc
    94981 tcagtcgatt tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat
    95041 ggctgctatg atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt
    95101 tggttttgct caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc
    95161 gccagtattg ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt
    95221 agggaaagct tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt
    95281 tagcgaataa aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa
    95341 ggcaataaaa ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc
    95401 atggattcag taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct
    95461 gatacattag gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt
    95521 ggtttaggaa cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat
    95581 gcgacgaaat tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc
    95641 atgggattga atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg
    95701 atcaaacaaa tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga
    95761 acagctgtta ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat
    95821 acttctgccg atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa
    95881 ttatatcgga tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc
    95941 cgaatcattg cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc
    96001 catgattatg cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga
    96061 aaggacgtga ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa
    96121 tctgtccgca cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac
    96181 gatttttcag gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt
    96241 cgggatgtag tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg
    96301 cttgaagcga tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg
    96361 acgtttactg gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa
    96421 atgaaagaac aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct
    96481 gagaacttta gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa
    96541 tctttttaaa taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc
    96601 agtttatcct tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat
    96661 taggtgaatt agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt
    96721 tctttcctgt ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag
    96781 gcaagaaagt gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa
    96841 cagtgaccta tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta
    96901 atgcatgtgg tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc
    96961 atatcaattt tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta
    97021 gatttccaga tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag
    97081 cagaaaaact aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt
    97141 atgaaacgcc tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt
    97201 cgactgttcc agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt
    97261 gtattaccaa cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa
    97321 caactcagag agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt
    97381 aataggagga acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat
    97441 aagattttac ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa
    97501 gatccagttt gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga
    97561 gaacgtattt ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat
    97621 gaattgatcc agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg
    97681 tcaaaagacg tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg
    97741 atgatcgaaa aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta
    97801 ctgaaagcat atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt
    97861 tcggaagatt ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc
    97921 gttttaggag tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg
    97981 cagacattaa gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct
    98041 gcagaacgtc aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca
    98101 gaataagaag gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa
    98161 tagctcctca tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac
    98221 cgtttaccaa gaggttgtga atctgccgtt taactctgag tcacaaggaa caatttcaaa
    98281 aaacagcttt tcatgtttga tgaatattgt gacttgtgac cgagaagttg gcaggagaaa
    98341 accgtttata cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt tttatagtgt
    98401 tttttatagg tagtattttt tagcaactgt taagtcatca tttaattcat aaacaagtgg
    98461 ttgacctgtt ggaatttcaa gatccatgat gtcttcgtca gagatacctt cgatatgttt
    98521 agctagggcg cgtaatgagt taccatgtgc agctactaaa acagttttgt tgtctagtaa
    98581 tgctggagca atttcatctt gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt
    98641 ttctccacca ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga
    98701 accttcgtca gtttcttcca ttaatggagg aagagtatcg taagaacgac gccagatgtg
    98761 cacttgttcg tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt gtaatttacc
    98821 atagtgacgt tcgtttaagc gccatgattt gatttgtggt acccaaagtt gatctgaata
    98881 ttctaagatc aagttacatg ttttgattgc acgagttaaa acagaagtgt aagcaacatc
    98941 aaattcgatt ccagcttctt tgattttgcg tccgccttct tttgcttctt cgattccttc
    99001 tggtgctaga tttacatcag cccaaccagt aaattggttc aatgcattcc attcactaag
    99061 accgtgacga gaaaaaacta attttggcat gtttagctct ccttttaaga taggtttata
    99121 tttctgaaat tttcagttct gttttattgt acaatttttt ggaagagatt tccatcgaaa
    99181 aattagataa gattctatga gaaaacaagt agagaatata aaaaaacgaa cagtgcccac
    99241 gcaactgttc gtttttaggt attagatgca taattgctcg atttcttttt caggtaaatt
    99301 gatatattta taacatgttc caactgaaac accgcatttt gaagaaataa attggatcgt
    99361 ttcttttttt tcataataga gacgtcggat ttttttgatc gtttttgcat cgatttttgg
    99421 tcttccgccg atttttcctt ttttacgagc ttcatctaat ccaactaatg tacgttcttt
    99481 gatcaatgcg cactccatgt ttgctaggtt ttccattaat tggaaataga tttttcccat
    99541 ttcgcgcgta tcgattcctt catcaagact aatcaagtgc agcccgcgat ctttgaataa
    99601 ttgcgtgagt tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac
    99661 tagtgcatcg ccttgttgca tgtcagcgat cactgactcc aaatgaacag cagtagaatc
    99721 attttgttcc acttcatatg tctcatggaa aatttcttga caaccaaagt cagataattg
    99781 atcaacctga gttgttaaat caacgtcatt tatggttgtg cgtgcataac caataatttt
    99841 catggtgatt cctttctagc agtgattttc taactggata taacagtaaa aatgtagcat
    99901 aaagtacgtc aaaaagatag ttttttcgca aattttccgc tgtctttttt tattggatga
    99961 tcaagcagtt tttctttatt tacgacggat tgcgtcaaga atctcaacgc ctttttctga
   100021 gccgacaact actttagaaa ccatgtttag gaaaagccca tgttctacta ccccaacaag
   100081 ttcatctaaa taggtagcta agctttctgg ctgttcgatg acttctagat gcagatcaat
   100141 gatgtaatga ccaccatctg tggttaagac ttctccgttt tcgtctgtac gcaagacggg
   100201 ttggaatctt ttttcttcaa aaatatgaag tagctgttgg gagccataag ggatcacttc
   100261 aactgggagt gggaacttcc caagtttatc gactaattta gaagaatcaa cgatccaaat
   100321 cgtttctttt gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt
   100381 gatcccttga aaatcagcac tgatttcatc agcaccgtct attgtcaaat cgactacggg
   100441 gacttcatca atactcttta acggtattcc taatttttcc gcttgttctt ttgtcgcatt
   100501 tgaagtagta actcctgtga tacgtaaacc ttcttcacgc atacggcgac caatctcttc
   100561 taccatataa taggcggttg aaccggtccc gagacctacg atcatcccgt cctttacgaa
   100621 tttcgcggct tcaatgccag ctaattgttt taaattcatc tttgcgtcca ccttttttta
   100681 gtttgagcat cgtgctttcc tcactatttt agcgtgattt acggtaaaaa gataccattc
   100741 ttttttattc caataaagct ttttcaaaaa aaattgcatc tcttgttgac atgttttttc
   100801 attcgtgata ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac
   100861 tgtttatcaa agcatatttg ataaggatgc agacattgca tttatttttt atcacaaaaa
   100921 aagaaccacc tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa acaaggaatg
   100981 cctacaatta accaattagt acgtaaacct cgtaaatcaa aggtggaaaa atctaattca
   101041 cctgctttga acaaaggata taacagcttt aagaaaactc aaactaacgt aaactcacct
   101101 caaaaacgtg gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct
   101161 ttacgtaaat atgcacgtgt tcgtttgtca aacttaattg aagttactgc ttatatccca
   101221 gggattggtc ataacttaca agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa
   101281 gacttaccag gggtacgtta ccatatcgtt cgcggtgctt tagatactgc tggtgttaac
   101341 gaccgtaaac aaagccgttc taaatacgga actaaaaaac ctaaagctta attttaatta
   101401 ttaaacaaat gaaacttgct tctactatat agaagtaatt actatgaaca atatttcgga
   101461 aggaggagtt acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc
   101521 ctatttataa ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac
   101581 gcgggattgc agctaacatt atctataatg cctttgactt gatcaaagaa tctacaggta
   101641 acgatccatt agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta
   101701 aagcacgtcg tgttgggggt tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc
   101761 gcgttactct aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt gaacacacaa
   101821 tggaagaacg cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt gcttctgtta
   101881 aaaaacgtga agacactcat aaaatggctg atgccaaccg tgcgtttgct cactatcgct
   101941 ggtaagattc tctctgtttg gtcggctttt ataagtcgac ctcagatagt gtccttattt
   102001 ttagcaacta gatagtaaag agaggagaaa caaggagaat gaccagagaa ttttctttac
   102061 aaaacactcg taatatcggt atcatggccc acatcgatgc tggtaaaacg actgctactg
   102121 aacgtatctt gtactatact ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt
   102181 cacaaatgga ctggatggaa caagaacaag aacgtggtat tacgatcact tctgctgcta
   102241 ctacagcaca atggaagggt caccgaatca acatcatcga cacaccagga cacgtagact
   102301 tcacagttga agttgaacgt tcattgcgtg tacttgatgg tgctgtaact gttcttgatg
   102361 cacaatcagg tgttgaacct caaactgaaa cagtttggcg tcaagcaaca acttatggtg
   102421 tacctcgtat cgtatttgca aacaaaatgg acaagatcgg tgctgacttc ttatattcag
   102481 tatcaacttt acatgaccgt ttgcaagcaa acgctcaccc aatccaatta ccaattggtg
   102541 cggaagacga cttcacagga attatcgacc ttgtaacaat gaaggctgaa atgtatacaa
   102601 acgatcttgg aacagagatc gaagaaacag aaattcctga agaataccgc gaattagctg
   102661 aagaatggcg cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa cttactttga
   102721 aatacttaga aggtgaagaa atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa
   102781 cagttaacgt agaattctac ccagtactat gtggttcagc atttaagaac aaaggggttc
   102841 aattactatt ggacgctgtt cttgattact tgccatcacc gttagatatc ccagctatca
   102901 aagggattga ccctaaaact gatgaagaag tagaacgtcc tgcagacgac agcgctcctt
   102961 tctcagcttt agcatttaaa gttatgactg acccattcgt aggtcgttta actttcttcc
   103021 gtgtgtactc tggtgtttta caatcaggtt catacgtaca aaacgcaaca aaaggcaaac
   103081 gtgaacgtgt tggtcgtatc ctacagatgc atgccaactc tcgttcagaa atctctgaag
   103141 tttacgctgg agatatcgct gctgctgttg gtttgaaaga cacaactact ggggacacac
   103201 tatgtgatga aaagagtcta gttattcttg aatccatgga attcccagaa ccagttatcc
   103261 aagtcgctat tgaacctaaa tcaaaagctg accaagataa aatgggtgtg gctttacaaa
   103321 aactatctga agaagatcct actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa
   103381 tcatcgctgg tatgggtgaa cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat
   103441 tcaaagttga agctaacgta ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg
   103501 gcactcaagc cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg
   103561 tatggattga atttacacca aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg
   103621 tcggtggggt tgttcctcgt gaatacattc cagctgttga aactggtttg aaagatgcta
   103681 tggaaaatgg tgtcttagct ggctatccat tagtagatat caaagctaaa ctttacgatg
   103741 gttcatacca tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct tctatggcat
   103801 tacgtgcagc agctaagaaa gctaatccag taatccttga accaatcatg gctgtagaag
   103861 ttgttatccc tgaagattat ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac
   103921 gtgttgaagg tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg atggttccac
   103981 ttgctgaaat gtttggttat gctacaactc ttcgttctgc tactcaaggt cgtggtacat
   104041 ttacaatgac atttgaccac tacgaagatg taccaaaatc agtacaagaa gaaatcatta
   104101 agaaaaacgg cggaaaagct gaataatttc ttaaaaccgg atagtcctgc aaagagatta
   104161 tccaaagaga ctatattttt aatatagttt cgtgtaaaat aattatcgat gatatgggca
   104221 tgtggtgtac cttgtgtaca tcatatacct atcagacaaa taaaaaatat attgtatttt
   104281 taggaggaca ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca
   104341 ttggtactat cggacacgtt gaccatggta aaactacatt aacagctgca atcacaactg
   104401 tactatctaa gaaaaacggc ggccaagcta tggcttacga tcaaatcgat ggtgctccag
   104461 aagaacgcga acgcggaatc acaatctcaa cagctcacgt tgagtatgaa acagatacac
   104521 gccactacgc tcacgttgac tgcccaggac acgcggatta cgttaaaaac atgatcactg
   104581 gtgctgctca aatggacgga gctatcttgg tagtttctgc tgctgacggc ccaatgcctc
   104641 aaactcgtga acacatccta ttgtctcgtc aagttggtgt tccttacatc gttgtattct
   104701 tgaacaaagt agacatggtt gatgacgaag aattactaga attagttgaa atggaagttc
   104761 gtgacctatt aacagaatac gaattccctg gtgacgatgt tcctgtagtt gctggatcag
   104821 ctttgaaagc tctagaaggc gacgcttcat acgaagaaaa aattcttgaa ttaatggctg
   104881 cagttgacga atacatccca actccagaac gtgacaacga caaaccattc atgatgccag
   104941 ttgaagacgt gttctcaatt actggacgtg gtactgttgc tacaggtcgt gttgaacgtg
   105001 gacaagttcg cgttggtgac gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa
   105061 caacagttac tggtgttgaa atgttccgta aattgttaga ctacgctgaa gctggagaca
   105121 acattggtgc tttactacgt ggtgttgcac gtgaagacat ccaacgtgga caagttttag
   105181 ctaaaccagg tacaatcaca cctcatacaa aattctctgc agaagtatac gtgttgacaa
   105241 aagaagaagg tggacgtcat actccattct tcactaacta ccgtccacaa ttctacttcc
   105301 gtacaactga cgtaacaggt gttgttgaat taccagaagg aactgaaatg gttatgcctg
   105361 gcgacaacgt aacaatggaa gttgaattaa tccacccaat cgctatcgaa aacggtacta
   105421 aattctcaat ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag
   105481 cttaattcac ttaagtttta aagtaaagca acatttattc ggacgtaatc gctcggaacg
   105541 aaggtcttag gatctttgtt ccgagctttt ttatttattg caggatcagg aaaaaagaca
   105601 ggagtattat aaatagaaga aacacgaaat aaaagagtca gtattttcta aatgaaactt
   105661 tatgaaaaca aaatgttttt tacaagaaaa agccttgctt tgtgagttta tatatattat
   105721 aatgacaaag gtgcgtttac tttagtgaat tgcacagacg taatcagaat gatttcgtcg
   105781 gctaagaaac gagaggttgc gacacgcccg gacgctttgc cacgaacgag cgtgacggaa
   105841 attttcgtgg agctatgtct acttttcaaa ataggcgaag gagggaacaa gatggcaaaa
   105901 caaaaaattc gtatccgttt aaaagcgtat gaacaccgta ttttagatca atcagcggat
   105961 aaaatcgtgg aaactgcaaa aagaactgga gctagcgtgt ctggtccaat tccattacca
   106021 actgaacgca gtctttacac aatcatccgt gcgactcata aatacaaaga ttcacgcgaa
   106081 caattcgaaa tgcgtacaca caaacgtctt atcgacattg tgaacccaac accaaaaact
   106141 gttgatgctt tgatgaagct agacttacca tctggtgtaa acatcgaaat caaattataa
   106201 aactaaaaca aaaataatgg aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg
   106261 ggaatgacac aaatcttcac tgaatctggt gaattaattc cagtaactgt tgtagaagct
   106321 actccaaacg tagtactaca agtgaaaaca atggaaacgg atggctacga agctattcaa
   106381 gttggttacc aagatatgcg tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca
   106441 aaagcaaaca cggctcctaa gcgcttcatt cgtgaattca aaaatgttga gctaggagaa
   106501 tatgaagtag gaaaagaaat tacagtagat gttttccaag caggagacat tattgatgta
   106561 acaggtacta cgaaaggtaa aggattccaa ggggttatca aacgccacgg acaaagccgc
   106621 ggaccaatgg ctcacggttc tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca
   106681 cctaaccgtg tatttaaaaa caaacgtctt gctggccgta tgggtggcaa ccgcgtaact
   106741 atccaaaact tggaagttgt acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt
   106801 aatgttcctg gagctaaaaa atctttaatt actatcaaat cagctgtgaa agctaaataa
   106861 ttgtaggaag aaaggaggaa ctaaggaatg ccgaatgtag cattattcaa acaagatgga
   106921 agccaaaacg gtgaaatcac acttaacgaa gaaatcttcg gaattgaacc taatgaaagt
   106981 gttgtttacg atgcaatcgt aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt
   107041 aaaaaccgta gcgctgttcg cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt
   107101 cgtgcacgtc aaggatctat ccgctcacca caatggcgtg gaggtggcgt agtctttgga
   107161 ccaacaccac gttcttacag ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa
   107221 tctgtattgt cagaaaaagt tgctgaaaac aacttggtag caatcgaagg cttgaacttc
   107281 gacgcaccaa aaacaaaaga attcaaacaa gttcttgcta acttatctat cgactctaaa
   107341 gtattggttg ttctagaaaa aggtaacgac tttgctgctt tatcagcacg taacttacca
   107401 aacgtttctg tagtaacttc tgataatgtg agtgtgctag acgttgtatc aaatacaaaa
   107461 gtgttagcaa cacaaactgc tcttactcaa attgaggagg tgcttgcata atgaacttac
   107521 tagacgtaat caaacgccca gtgatcactg aaaaatcaat gcttgccatg gatgacaaga
   107581 aatacacttt tgaagtggac actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag
   107641 ctgcttttga cgttaaagtc aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac
   107701 gtatgggcaa atatgcagga tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg
   107761 aagattcaaa agaaatccaa ttattcgaag ctgctgaata agcactaata gataactcat
   107821 taaattagga gggaaaaacg tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg
   107881 taacatgaca ggttctgatt ttgctgaaat cacaacatca acacctgaaa aatcattgtt
   107941 acaaccattg aaaaaccatg ctggacgtaa caacaacggc cgcatcactg tacgtcatca
   108001 aggtggcgga cacaaacgcc aatatcgcgt gatcgacttc aaacgtaaca aagataacgt
   108061 tgttgcaact gttcaaacga tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt
   108121 acactatgaa gatggagtga aagcttacat cttagcacct aaaggactag aagtgggtat
   108181 gaaacttgtt tcaggtccag aagcagatat caaagtcgga aatgctcttc cattagaaaa
   108241 cattccagtt ggtactgtta tccacaacat tgaaatgaaa cctggtaaag gtggacaatt
   108301 gatccgttct gctggtacaa gtgctcaagt acttggtaaa gaaggcaaat acgtattgat
   108361 ccgcttgaac tctggtgaag ttcgtatgat cttagctgca tgccgtgcta caatcggttc
   108421 tgtaggtaac gaacaacacg aattaatcaa catcggtaaa gctggacgct ctcgttggat
   108481 gcgtaaacgc ccaactgtac gtggtagcgt aatgaaccct aatgatcacc cacacggtgg
   108541 tggtgaaggt aaagctccta tcggacgtaa agcaccagtt tctccttggg gtcaaccagc
   108601 tcttggttac aaaactcgta acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg
   108661 caaaaataaa taattacttt gaccatgtgt cgcacatttt gagaggaggt tcaccatggg
   108721 tcgtagttta aagaaaggac cttttgtcga tgagcattta atgaaaaaag ttgaagctca
   108781 agcaggggtt gaaaagaaaa aagttatcaa aacttggtca cgccgttcaa cagttttccc
   108841 aagttttgtt ggatatacca tcgcagttta tgatggacgt aaacatgtac cagtttacat
   108901 ccaagaagac atggtaggac acaaattagg tgaatttgca cctacaagaa cttatcgtgg
   108961 acatgctgct gacgataaga aaacgaaacg ctaatttgag gggaggataa atccaaatgg
   109021 cagaacaaat cacatcagct aaagcaactg caaaaacagt tcgcacttca cctcgaaaag
   109081 cccgtttagt aattgacctt atcagaggta aaagtgtcgc agatgcaatt tcaatcttga
   109141 aattcacacc aaacaaatct gctggaatca ttgaaaaagt attgatgtca gcagttgcga
   109201 atgcagaaaa taactttgac ttagatgttg aaaacttggt agtaagcgaa gcttttgtta
   109261 acgaaggacc tacaatgaaa cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca
   109321 acaaacgtac aagtcatatc acggtagtcg tatcagaaaa ataaggaggg ataatcagtg
   109381 ggtcaaaaag tacatccaat tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa
   109441 tggtatgctg aaaaagagta tgctgagttc ttacacgaag atttaagaat ccgtaaattt
   109501 atttcaacta aacttgctga tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt
   109561 cgcgtaaaca tttcaattca cacagctaaa ccaggtatgg ttattggtaa aggcggttct
   109621 gaagtcgaaa gcctaagaaa agaattaaac aaattaactg gtaaaagaat ccatatcaat
   109681 atcgtggaaa tcaaaaaacc agatctagat gcaaaattag taggtgaagg aatcgcacgt
   109741 caattagaaa accgtgttgc tttccgtcgc gctcaaaaac aagccatcca acgctcaatg
   109801 cgtgctggcg ctcaagggat caaaactcaa gtatctggtc gtctaaacgg agcagatatc
   109861 gctcgttcag aaggatactc agaaggaact gttcctcttc atactttacg tgcggacatt
   109921 gattacgcat gggaagaagc agatacaaca tacggaaaac taggagttaa agtgtggatc
   109981 tatcgtggag aaattcttcc aactaaaaaa aacactgaga aaggagggaa ataatcatgt
   110041 tagtacctaa acgtgtaaaa caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta
   110101 aaggcggaaa agaagtagca ttcggtgaat acggtttgca agctgttgat tcacattgga
   110161 tcacaaaccg ccaaatcgaa gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg
   110221 ggaaagtatg gattaaaatt ttccctcaca aatcttatac tgccaaagca attggggtac
   110281 gtatgggttc tggtaaaggg gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa
   110341 tcatgtttga aatcgcaggc gttcctgaag aagtagctcg tgaagcgtta cgtctagctt
   110401 ctcacaaatt accaatgaaa actaagatcg taaaacgtga ggaaatgggt ggtgaatcga
   110461 atgaaggtta aagaaatcag agaattaacc actgccgaaa tgcttgatca agaaaaacaa
   110521 ttaaaagaag aattgtttaa tcttagattc caattagcaa caggtcaatt agaaaacact
   110581 gcacgtatta aagaagtacg taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa
   110641 gctaagtaat tatggaagga ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa
   110701 gtttatcaag gtcgcgtggt ttcagacaaa atggataaaa caattaccgt tgctgtcgaa
   110761 acaaagaaaa accaccctat ctatggcaaa cgcatgaact attctaagaa atacaaagtg
   110821 catgatgaaa acaacacagc aaaagttggc gacatcgtga gaattatgga aactcgtcca
   110881 ttatcagcta caaaacgttt ccgtttgttg gaagtagtcg aagaagcagt tattatctaa
   110941 taacacgaga ttttcctata tagattgaat gcaaaatctg aaaggaggat acacaacgtg
   111001 atccaagcag aaagtcgttt aaaagtcgct gataactcag gcgcacgtga aattttgacg
   111061 atcaaagtcc ttggtggatc tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct
   111121 acggttaaac aagcaacgcc aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt
   111181 atcgttcgaa caaaatcagg agctcgtcgt acagacggtt catatattaa atttgatgaa
   111241 aatgctgcag tgattattcg tgatgataaa agccctcgtg gaacacgtat ctttgggcca
   111301 gttgcacgtg aactacgtga aaacaacttc atgaagatcg tttcactagc accagaagtt
   111361 ttataatctt gagacactta tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc
   111421 gataaagtta aagttatcac tggaaaagac aaaaacaaag aaggcgttgt actagcagcg
   111481 ttcccgaaaa aagataaagt tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa
   111541 ccttctcaag ctgctcctca aggtggaatt gttgagatgg aagcgccaat tcatgtttct
   111601 aacgtgatgg tggttgactc tactggtgta gctggccgag ttggctacaa agaagtagac
   111661 ggcaaaaaag tccgtgtttc taaaaaaacc ggtgaagttt tagataaata aaagtttaag
   111721 gaaggagggt cttactgaat gaaccgccta aaagaaaaat atcttaaaga agtaactcca
   111781 tcattgatgg aaaaatttaa ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc
   111841 gtcatcaaca tgggtgttgg tgatgcggta tcaaacgcta aaaacttaga taaagcagtt
   111901 gaagaattaa ctttgatcac tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc
   111961 gctggattcc gtttacgtga aggaatgcca attggtgcaa aagttacatt gcgcggagaa
   112021 agaatgtacg aattcttaga caaattggtt tctgtttcac taccacgtgt acgtgacttc
   112081 catggtgtaa gtaaaaaagc ctttgacggt cgtggaaact atactttagg tattaaagaa
   112141 caattgatct tcccagaagt agattatgat ttagtagata aagtacgtgg tatggacatc
   112201 gttattgtaa caacagcaaa ctctgatgaa gaatctcgtg agttgttgac acaattaggc
   112261 atgccattcc aaaaataatt aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct
   112321 aaaaacaaac gtcctgcaaa attttcaaca caagcttata ctcgttgcga acgttgcgga
   112381 cgtccacatt cagtttatcg taagttccat ctttgccgta tttgcttccg cgaacttgcc
   112441 tataaaggtc aaattcccgg cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag
   112501 aaggaggtaa aagatcaatg gtcatgacag atccaattgc agattttcta actcgtatcc
   112561 gtaacgcaaa catggttaaa catgagtctt tagaagtacc tgcatcaaaa attaaacgtg
   112621 acatcgctga aatcttgaaa cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg
   112681 acaaacaagg cgtgatccgt gttttcctaa aatttggtaa aaacggcgaa cgtgttatca
   112741 caaacttaaa acgtatctct aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac
   112801 caaaagtatt gaacggttta ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg
   112861 ataaagaagc tcgtgaaaaa aatatcggcg gcgaagttat cgcttacgta tggtaatcaa
   112921 gtagaatcta caaggaggtg tctttaagtg agccgtatcg gaaataaagt cgttgttatc
   112981 cctgaaggag taacagttac tcaagacgga aacaacgtta cagttaaagg accaaaaggt
   113041 gaattgactc gtactttttc agcagatatc aaaatgaata tcgaaggaaa cgaagtaact
   113101 ttcactcgtc caaacgacag taaagaaatg aaaacaattc atggaacaac tcgtgcgaac
   113161 ttcaacaaca tggttgtagg tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg
   113221 gttgggtacc gtgcacaatt acaaggatca aaacttgtat tgaacgttgg ttactcacat
   113281 ccagtagaaa tcacaccacc agctggagta actgttgaag taccagctaa cacacaagta
   113341 attgttaaag gtgcgaacaa agaagaagtt ggcgaattag ctgcaaacat tcgtggaact
   113401 cgtcctccag aaccttataa aggtaaaggt attcgttatg ttggcgaata tgtacgccgt
   113461 aaagaaggta aaactggtaa ataatcgttt agaaaaattg catagaagcc agcagcagga
   113521 aaaaggcatt tggctgaagt ttaccttcaa aaaaatacat ttttacttgt gccgcagctt
   113581 ctatttttcg aaacgtattt actgcttgaa atagtagtac aataaaaata aagaggtgac
   113641 aattgtgatt acaaaaccag ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg
   113701 taacaatatc tctggtactg ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa
   113761 catctacgct caaattattg atgacgtagc gggtgtaacg ctagcaagtg cctctacctt
   113821 ggataaagaa atttcaggtg gaacaaaaac agaagcagca caagctgtcg gtaaactagt
   113881 tgctgaacgt gcagctgaaa aaggcattaa agtagtagtc tttgaccgtg gtggatacct
   113941 ttaccatggc cgtgtgcaag ctttagctga agctgctcgc gaaaatggac tagaatttta
   114001 gaagaaggag gaataccatt catggtttat atcgatccaa aacatttgga attagaagac
   114061 cgcgttgttg caattaaccg tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt
   114121 gctgctttag ttgttgtcgg tgacaaaaac ggacacgtag ggtttggtac aggtaaagct
   114181 caagaagttc ctgaagcaat ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa
   114241 gtacctatgg ttggttcaac aatcccacac gaagtaatcg gtgcttttag tggtggtaga
   114301 atcctaatga aacctgctgt agaaggttct ggggttgctg ctggtggacc agttcgtgcc
   114361 gtattagaat tagctggggt agcagatatc acttctaaat ctttaggctc aaatacacca
   114421 atcaacgttg ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt
   114481 gcagaacttc gcggcaaatc agttgaagaa ttaatcggat aaggaggaca acaataatgg
   114541 ctgaattaaa agttacttta aaacgcagta ttatcggacg tcctcaaaac caacgcgata
   114601 cagttaaagc gttaggtctt acaaagatta acagttcagt ggttaagcct tctaatgaag
   114661 caattaaagg catgatcaat actgtttctc acctagtgga cgttgaagaa atttaattta
   114721 gaaaaatcat aactagaaca ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa
   114781 cctgctgaag gttcacgcca agtacgtaac cgtgttggtc gtggtacttc atctggtaac
   114841 ggaaaaactg ccggacgcgg acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt
   114901 ttaggatttg aagggggaca aacaccattg ttccgtcgtt taccaaaacg aggattcaca
   114961 aacgtgaacc gtaaagacta tgcagtcatt aatcttgatg tattgaaccg ctttgaagac
   115021 ggagctgaag taactcctgt tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct
   115081 ggaatcaaag tattagctaa tggggaacta aacaagaaat taactgtgaa agcagcaaaa
   115141 ttctctaaag cagctcaaga agcaattgaa gcggctggtg gatcaatcga ggtgatctaa
   115201 tgtttagact attaaaagat gcttttaaag taaaagacat tagatcgaaa atcctattta
   115261 ctgtatttgt gttgtttgta tttcgcctag gtgcacatat tactgtacca ggcgtaaatg
   115321 ctgcttcatt gcaaaacctt tcttctttgc catttttcaa catgttgaac ttggtaagtg
   115381 gtagtgcgat gcaaaacttt tccattttct caatgggggt ttcaccatac attactgctt
   115441 ctatcgtcat tcagcttctt caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac
   115501 aaggggaagt tggtcgtaaa aaactgaatc aagcgactcg atatctgaca ttagtattgg
   115561 catttatcca atctgtcggt atcactgctg gattcaatca atacgctcaa ttaggtttcg
   115621 tgaataatcc taacgtagca acttacgtaa cgatcggttt gattctaaca gccggtacga
   115681 tgtttgttac ttggctagga gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa
   115741 tgatcatttt tgcaggtatc atttctagat tgcctgaaag tatcagagaa atttatgaag
   115801 attactttat aaatattgat ccttcagaca tatggaaatc tgctattttt gtggccattt
   115861 tactagtagc ggtcttgttg atcgtgacat tcgtaacatt tttccaacaa gctgaacgca
   115921 agatcccgat ccaatatacg aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt
   115981 tgaaagtaaa tgcagctgga gttattccag ttatctttgc tagttcgttt attgctacgc
   116041 caaatgctgt tttacaagca ttgagcggta gattcggttc tgaaaactgg tacaaagtga
   116101 tgatggagat ctttaactat aatacagcac ctggtgctac gatttatacg attctgatcg
   116161 ttgccttcac tttcttctac gcctttgttc aagtaaaccc tgagaaatta gcggaaaact
   116221 tgcaaaagca aggaagttat ataccaagcg tacgccctgg taaggggact gaagagtatg
   116281 tctcaagatt actgatgcgt ttaagtacag ttggtgcagt cttccttggt ttagtggcat
   116341 tgcttccaat cattgctcaa atggtttggg atcttccaca atcaatcgga ttaggcggta
   116401 caagcttact gatcgttatc ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa
   116461 tgctgaaacg taaatatgtt ggattcattg agtaagaaag cgtgttgagg tttcccctca
   116521 acacacgttc tcacacattt attaggaggg aaaacaatga acctcatttt aatggggctg
   116581 ccaggcgcag gtaaaggaac acaagctgaa aaaatcattg atgcttatgg tattccgcac
   116641 atctcaacag gagatatgtt ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa
   116701 gcaaagtctt atatggataa aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg
   116761 aaagagcgac tagctgaacc agatacagag aaaggcttct tattagatgg ctttccacgt
   116821 acgctagatc aagcaaaagc tctggacgca atgctaaaag atttgaataa aaaaattgat
   116881 gctgtcatcg atatccatgt tggtgaagaa atcttggtcg aacgtctagc cggccgattt
   116941 atctgcagca attgtggagc aacttaccat aaaattttca accctacaaa agttgaagac
   117001 acatgtgatc gttgcggtgg gcatgaattc tatcaaagag aagatgataa acctgagacg
   117061 gttaaaaatc gtctggctat caacatcaaa aatagtgaac caatcttggc ttattataaa
   117121 gaacaaggtt tgctaaacac tattgatggt gctcgtgaaa tcgatgctgt atttgcagat
   117181 gtacaaaaaa tcattgaaaa atagcacgat tgcccgtagc cgacttgcat tatgcagtta
   117241 ctcttgtcaa gttcaataaa ttatgataga atatgtcagt ccgacttcta gagaatcctc
   117301 tagatgatcc attttgagaa ttttgaggtg ggaactgcag tttccgccat tttatcgtgt
   117361 gaaaatgcga aacaagtaca aggaggtact gtgcgtggca aaagaagata tgattgaagt
   117421 cgaaggtaca gtcgtcgaaa ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg
   117481 acaccaagtt cttgctactg tttcaggtaa aattcgtatg cattacatcc gtatcttacc
   117541 tggtgacaaa gtaacggttg aattgtctcc atacgatctt aaccgtggcc gtattactta
   117601 tcgctttaaa taattgtact ccgtaaaact acaggaggta ttatcatgaa agtaagacca
   117661 tcagtaaaac caatgtgcga acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg
   117721 atttgcccag caaatccaaa acataaacaa cgtcaaggat aattggaggt gctataaata
   117781 tggctcgtat tgcaggagta gatattcctc gtgataaacg tgtagtagtt tcacttactt
   117841 atatctatgg tattggtaac actactgcta aaaagatctt agctgatgct ggtgtatctg
   117901 aagatgttcg cgttcgtgaa ttaacgaatg aacaaacaga tgctatccgt gcagaagtag
   117961 ataaattaaa agttgaaggg gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga
   118021 tggaaatcgg ttcttaccgt ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa
   118081 acacgaaaaa caatgcacgt actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa
   118141 aataattctt aagtgaagga ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac
   118201 gccgtgtgaa gaagaatata gaagcaggta ttgcacatat ccattctaca ttcaataaca
   118261 ctatcatcat gatcactgat actcacggaa atgcattagc gtggtcatca gctggttcat
   118321 taggtttcaa aggaagcaaa aaatcaactc cttttgccgc tcaaatggca gcagaaactg
   118381 ctacaaaagc agcaatggaa cacggattaa agactgttga agtaacagtt aaaggacctg
   118441 gttctggacg tgaagcagca attcgttcat tacaagctac aggtttagaa gtgactgcaa
   118501 ttcgtgacgt gactccagtt cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt
   118561 aatgagtgcc gctcattttg agttttatca gtaacgtcat tgaacaagac acttttcgtt
   118621 ttgaaagggg taaagataag aatgattgag ttcgaaaaac caagaatcgc aaaaattgat
   118681 gaagaaaaag attatggcaa gttcatcgtt gaacctcttg aaagagggta tgggactact
   118741 ttagggaatt ccctacgtcg tattttatta tcttctttgc caggtgctgc catcactaat
   118801 attcaaatcg atggtgtact gcatgaattc tctaccatca aaggtgttag agaagacgtc
   118861 actcaaatca tcttgaacat taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa
   118921 acccttgaaa tcgatattac cggacctgct acagtaactg ctggcgatat tatcgttgat
   118981 agtgatgtag aaattcttaa caaagatatg tacatctgta gtgtctctga aggagctacg
   119041 ttccatgctc gcttaacagt gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa
   119101 aaagaagata tgccaattgg cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt
   119161 gtgaactatc aagttgagaa cacacgtgtt ggacaccgtg aagattttga taaattgacg
   119221 atggagattt ggacagatgg ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa
   119281 atcatgactg aacatttgga tatttttgtg aacttaactg atgaagcaaa aaatgctgaa
   119341 atcatggtcg aaaaagaaga aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag
   119401 ttagacttgt ctgttcgctc ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa
   119461 gaacttacaa ataaatctga gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca
   119521 ttagaagaag ttaaagcaaa attgcacgac ttaggtttag gtttacgtaa agaagattaa
   119581 aaccttttac gaaggaggga aaccaacgtg agttatcgta aactaggacg cacatctagc
   119641 caacgtaaag cgatgttgcg tgatttaaca actgatttat taatcaacga acgtattgtg
   119701 actactgaag ctcgtgcgaa agaagttcgc tcaactgcag aaaaaatgat cactttaggt
   119761 aaacgcggag atttgcatgc acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc
   119821 agcgtgcgtg aagaaaatga ggacatcgtc attgaatcag ctttacaaaa actgttcaac
   119881 gatattgcac ctcgttacgc agaacgtcaa ggcggataca cacgcatttt gaagacagaa
   119941 ccaagacgcg gtgatggtgc accaatggtt gtcttagaac ttgtctaaaa tcacactaat
   120001 aacatcactt tatagatgat tcttttagag tgttatgatg ttggaagata tttttccgag
   120061 tctagctcag cgctgctcct caagcgaata tcgcttgggg agtaagcact catcataaag
   120121 tgttttttta taccttcaat tacctgtcat gacagatatg tttgaaggca taaaaaaaca
   120181 ttttaagcga gaaataattg aaaattttgt ctttttttga tagtgttagt aggaacttta
   120241 ggatgaagaa ggagtaatca tggagccaat tattgaatta gaaaagataa attataaata
   120301 ccagccagat gatcttcgcc ccgcattgaa agatgtctcc tttacaattg ataaaggtga
   120361 gtggatcgct attattggac acaatggttc aggaaaatcg acacttgcca aaacgatcaa
   120421 tggattgctt ctgccagaat cagggatcgt gaaagtggga aatcaaatat tggatgaaga
   120481 aaatatttgg acgatccgac agatggttgg aatggttttt caaaatccag acaatcagtt
   120541 tgttggttcg acagtagaag atgacgttgc ttttggtctt gaaaatcaag ggattcccag
   120601 agaagaaatg ctagtgcgtg tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc
   120661 ttcacgcgag cctgctcgtt tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt
   120721 ggttgcctta cgacctgata tcattatttt agatgaagca accagtatgc tagatccaga
   120781 aggtcgtgaa gaagtgatct ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt
   120841 tatatctatc actcatgata ttgatgaagc agctaatgcc aatcggatac tagtgatgag
   120901 acaaggcgaa cttgtccgtg aaggtacacc aaaagaaatt ttttctgctg gtcctgaact
   120961 gatcgactta ggtctagatc taccatttcc tgaaaaactg aagagcgcac tgaaagaacg
   121021 tggggttgat gtgcccagtg aatatatgac agaagaaagg atggtggatt ggttatggac
   121081 atccgttttg aacaagtaga cttcacttat cagccgaata caccatttga acaaagagcc
   121141 ttatttgaca tcaatatgac gatcaaagaa aacagttata ctgcattagt agggcatacc
   121201 ggaagcggga aatcaacctt acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt
   121261 acagtccata ttggagaacg agatattcag ccggatacgg ataataagaa tttaaagccc
   121321 atccgaaaaa aagttggcat cgtctttcag tttccagaag cacagctatt cgaagaaacg
   121381 gtagcaaaag acattgcttt cggtcctaaa aactttggtg tcagcgaaga agaagcacta
   121441 gtcctagcaa aagaaacatt agaacaagtt gggctggatg aaagctattt ggaacgttcg
   121501 ccatttgaac tttcaggagg gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg
   121561 aggccggaag tacttgtatt ggatgaacca acggcaggac ttgatcccca gggcagaaaa
   121621 gaaatgatgg agatgttttg gcgtcttcat aaggaacagc agatcactat tgtattagtt
   121681 acgcatttaa tggatgatgt agccaatttt gcagattatg tctatgtgtt ggaaaaggga
   121741 cgaatcgtta atagcgggga gccgcaagag gtctttcaaa acattgaatg gctaaaagaa
   121801 aaacaactag gcgtacctac tgccaccgag tttgcagaag aattaatggc taaagggatg
   121861 aactttgcta ccttgccttt gacagctgaa gaattagcag acgctatcgt ccattatgct
   121921 ggaggaaatg cccatgatga ataaattgat atttggtcgt tatatgcctg gagattcctt
   121981 gatccatcga ttggatcccc gagctaaatt attagccagt ttttatttta tcggaatcat
   122041 ttttttagca aataattggg aaacgtacct tttgttggca tttttcacat tactttctgt
   122101 cttcttgtca aaagtagatt tgaaattttt tatccgaggc attcgcccat tgatctggct
   122161 aatcctcttc actgtagctt tgcaggtctt gtttacaagt ggcggagaaa cttattggag
   122221 ttggggtgtt ttccatatca ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg
   122281 attcgtgttg atcattttta tgtcgactct actcacgctt actacaccgc ctttggaatt
   122341 atcggatgcg attgagtact tgcttcgtcc attgaaagcc gtacggttcc cagtccacga
   122401 aatttcattg atgctgtcga ttgctcttcg ttttgtgcct acattgatgg acgaaacaga
   122461 aaagatcatg aacgctcagc gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca
   122521 gaaaatgaaa gcagtcattc ctttgctgat tcctttattt gtcagcagct tcaaccgtgc
   122581 ggaagatctt gcgactgcta tggaagcaag aggctacaaa ggtggggaag gaagaacgaa
   122641 atatcgtgta ttgcattggc ataatagaga cacgatggtt atcattgctt ttgtattatt
   122701 aactgtgcta ctgatctttt taagaggata aacgttcagt gggccgctat ctattctttt
   122761 cttgctgatc tgtttgcaag aaaggatgca agcagtgtat gacaagaaat attagggtct
   122821 gtggcagact aaggtcacag gcctctttct tttatctgaa aagaaagaac gaaaaaattt
   122881 tatagaaaaa tcattattcc agataacctt ttaggaacaa aggagagaaa aacatggtac
   122941 gttacaaagc aatcattgcg tacgacggaa caaattttaa tggattccaa aaacagccaa
   123001 atgggcgtac tgttcaagaa gaagtagaaa aaacattaca aaaaatggca aatggaaaag
   123061 aaatcacggt ctttggttcg ggaaggacag atgccggagt acatgcgatc gggcaagtaa
   123121 tccattttga ttatccagag gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc
   123181 agtcaccaga ggatattgct gtaagacaag tagaaattgt ttcagaagac ttccacgcaa
   123241 gatatttggt caaagaaaag acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc
   123301 cttttcgacg ccattatgca agttattttc cttacccact tgatttggaa aaaattcagc
   123361 gggcgcttcc tgatttatta gggactcacg atttcacctc tttttgtgca tcaggaagct
   123421 cgatagagga taaaatacgt acgatctacg aggctaagat ggaggtaaat gaaacaaaag
   123481 acgaattgtt gtttacattt aggggaaatg gcttcttgta taagatgata cggatcttag
   123541 taggaacgct tttgaaaatt ggcaatggcc gattgcctga agatagtatc ccagatatta
   123601 ttgcgaaaaa agatcgtaat gcagcaggac ctacagcaca ccctgaagga ctttatttgt
   123661 atgaagtgaa atatgaataa gaggttatga tagaagtggt cagcttcaag aaataagaag
   123721 gtatttacga aaattacttc tcaaaatttt gcaaaatttc ggcttatttt cgaagaagct
   123781 acttctggaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa
   123841 agagcaattt caaaaaacag cttctcatct ttgatgaata tcgtgacttg tggacgaggc
   123901 gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac
   123961 gttgttttga ttgcccttgg tgcggacagt ctgaacaggt tccatcgttt ctttattttc
   124021 tacgtcttct tctgtgtgtg tttcgtggtt gttcttctat tgctggatca atcgtgccat
   124081 aaaaggggcg attgcggtca aggtaccaga cataaagaat aaacaatgca atacatagga
   124141 caaaaaggat tgcacctatc gtaaatcctt caggcaagta actgaaagtg acagtatgtt
   124201 tacctgctgg aacttttatc atcaagaatg cgtctttgaa attgctgacc ttgactggtt
   124261 tcccatcaat tttagctttc catcctttgt cataaggaat agttgtcacc aacatggaat
   124321 cttcagcggt atttacagtc ccagaagctt ttctgccagt ggcttccatt tcgacacctt
   124381 tttctttgat ctgattcatt gcggcttgat aggctacaac atctagacca acgactttag
   124441 gctccatgaa acttatttct ttcgtcccat aaaaacttgc agtaaatgtc actgttgtct
   124501 cttgaggata atagccaaga tcatagtatt gaccagaaat gttgatttgc gtttttcgcg
   124561 atgtaccatt gacagtgatc gtcgcactag aactttctaa ttgcgcaaaa ttggttggga
   124621 acaaacttaa gtaagcttgt gtatttgcag gcacatggac ttcccaagtg agctcttttg
   124681 gctggttatc tgctgtttct gagtaagtga cgccagtcgt tgtctttgtg atcgtcgtat
   124741 tcaactgatt cttcaaagta ggtgtataga atgtaaaata ctgttcattt agaccagaca
   124801 atgtattgat caagtttgtc tgactggtca aattatctgt cactggctgc tcaacatcat
   124861 taacggacaa cggtgcagta aatgctagtg gcaatgcatt gacattctga tacagtgtat
   124921 agtcgccgct ttgaccagct tggcggaagc catatttact gaacgtgctt tcgtttttag
   124981 aaatgttata tttgattcct gtaaacccat ccatcagcaa agtattattg ttataacgga
   125041 tattcaaatt tgtccctctt gaacggaaac ctaatttatc caggtaagag gaagaattac
   125101 gatttcttat agaagaaaac aaactgatac cactgtaacc atagttgatc gaatcgtttg
   125161 cagatactgg atccagattt tccaagcggt aaaacgagtc ggaatctttt tttgtttcat
   125221 ttacaagagc tttcaagtca ggataaggat cagtatataa actgcgggaa gcataattcc
   125281 aatcatcaag aatgccatgg atcatactgt tcgtattgac cagtgcttca gcagacatga
   125341 tcaataataa caatatactc agatgattga atgaaagttt tttatgctga taaaatccca
   125401 cagcaaaagc ataaatccct aagaaaacca ctgtcaatac aaaggaagtc atcgtcacat
   125461 aggtgtagct tccttcaggt ttgatgcccc atgccaccgc aaagagagca gctacgacca
   125521 aaatgattcc taacaacagc ccacgatcgt cttgttctat cttttcccat ccttttcctg
   125581 caagcaggag cactaagaaa gagaacagat aggcataacg gaataaaaac atattcggtg
   125641 catgcatacc atgccagaac aaattcaatg gaacaagata aaaacttgcg ataagtatgg
   125701 cgaaaagact gccgtacaaa aatttatttt tcaacggtac tttccgactg gcaaaataaa
   125761 acagacagag gactaatggt agcagacctg cgtaaacgaa cggaatcgat ccgtattttg
   125821 tcgtgtcata aacaccaatc atatttttca tgattagatc cagataagaa gtcgcttctg
   125881 ttttcaacgt agtgatctga ctcagtgcct cgccatttga ccgaagatcc agcactgctg
   125941 gcaatacaat gatcattgaa gcgccgcctg ccagtaaaga agtgatacca taaggaacaa
   126001 tcgttttttt atattctgac cagtttgtca atagacgggc gataaaatat aagaaagaaa
   126061 atacgccaat cataaaaccc atataaaaac tggataaaaa caagagtaaa taactgataa
   126121 ataaaagttt cggccgttta agatccataa cacgatgaat ccccaaaatc actaatggaa
   126181 gataaacaaa tgcatccagc cacatgatga tctccgaatg tgcagtgata aatgagatca
   126241 atgcgtaact gacgctaagt gcaacatgcc cccagcgagg aatcttgaac gtacgtgcaa
   126301 gaaaccaaaa agccaatccg gcacagccga ccttaagcaa agtcagaaca tacagtgcat
   126361 ctggcatcat ctggtttgga aaaaagaaca ccagtggcgt gaagatcccg cctagatagt
   126421 aagaaattaa agataaataa ttcaatccta gagaagcatt ccatgtataa aaaatacttt
   126481 gctttccaag aagaacattt cggaagctgg catgaaaatt agaaaactgt gaaaaggcat
   126541 cacttgccaa aatacttctg ctgcttccag gatagattcc gatacttaga taaacaagtg
   126601 ctaaaatcaa aaacggaatc aggaaactag ctgctgcgta gcgtccgttt tctctaaaga
   126661 atgactttaa attttttttc atatttattt tccaccaaat ttctatgaag ttgtattcag
   126721 tttatcatat ccttgttttt ctctgcttaa aaagagtcta gttttcacaa aaaaataaaa
   126781 tttagaataa ataacttcat aatttgttcg tataacaaaa ataaacgcaa aaaaagggta
   126841 ggaatcaaaa gtcccaccct taaaaatttt tattaaataa aaaaccgttc actatctgaa
   126901 cggtccaagt atcattcaat taaacgcgtt caacttttcc tgatttcaaa gcacgagttg
   126961 acacccaaac ttttttaggt tttccgtcga ccataacgcg aactttttgt aagttaggtt
   127021 taacagtacg ttttgttgag ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc
   127081 gacctgtaaa gtaacaaact tttgccatgg taatttcctc ctttgccttg atttggagca
   127141 atagaataat tcagctcata ccagactaac atatcatata gaatgggaat ttgcaagtac
   127201 attttcatag atacaattga caattgagaa gaataattgt aaattaaaca aggttatacc
   127261 tgaaattttg caaggttttt gagtactttg ggtaactgcc ttttatttca agttgcgcta
   127321 tgataaaata tgatagaaga aaaatgggag ttttttaccc gttaaggagg ctcattcatg
   127381 gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta ccaatgatgt aattgcaaca
   127441 gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag gaatggccag caaaaatcaa
   127501 attaaagata atattactga gattttgcgt aaagaaaatt actcaagagg agtagttgtc
   127561 cgccaagaag ataatggtat tgctgtagat gtttatacga ttatcagcta tggtacgaaa
   127621 atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga ggcattatta
   127681 ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact gccagactag
   127741 gcaggagact ggctggtaac tcagcagtcg atctgaagga ggattatcta ggtgagtgta
   127801 acagaaataa gtgcaagcca gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa
   127861 gtaaatgcag aatacgtcaa ttcattaaat gtattccctg tgccagatgg cgatacggga
   127921 acaaatatga atctatctat gactagtgga gctaaggcag tagtagattc aacctcagaa
   127981 aaagtaggag aattagcagc aatgctttca aaaggattgc tgatgggtgc gcgcggaaat
   128041 tccggcgtta ttttgtctca attgtttcgt ggattttcta agcaaattcc agatgttgtc
   128101 tcattaaatg cttctgatct tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa
   128161 gcagtaatga aaccagtaga aggaacgatt ttgacagtag ctagagaagc agcgaaagct
   128221 ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag taatgacagc tgttgtaaaa
   128281 ggagcaaagc gtgctttagc taaaactcca gacttgcttc cagtcctaaa agaagtcggt
   128341 gtcgtagaca gtggagggca aggactgctg tttgtttatg agggcttttt aagtgcattg
   128401 aatggagaat tccaagcgga tgaaacatat gaaccttctc cagcagaaat ggatgatatg
   128461 gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt
   128521 ggttactgta cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga tagtacattc
   128581 gattacgaag aattccgtaa ctatttagat ggtataggag attctttact tgtggtcaat
   128641 gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc caggagaagt cttgaattat
   128701 ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag
   128761 acgattttgg aacacgatga agaagtgaac gagtttgcga aaaaagaaat accacgtatc
   128821 ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta
   128881 ggcgctaatt atgttatcag tggtggacaa acaatgaatc caagtacgga agacattgtt
   128941 aaagcgattc aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa taagaacatc
   129001 ttcatggcag ctgatcaagc agcagaagta gcagatatcc cagtagcagt tgttccttca
   129061 agaacgattt cccaaggatt gacagctatg ctttcattca atgatcaagc ttctttagaa
   129121 gaaaacaaag aaacgatgac agaaatgttg tctagcgttg tgagcggtca agtcactcac
   129181 gcgatcagag atacctcaat tgacggtgta gagatcaaag aaggagactt tttgggcatg
   129241 atcgatggaa aaatcctgat ctctaaccca gatatcacag caacagcgtt aagtacacta
   129301 gaacaaatga tcaatgagga cacagagatc gtaacgatct tggcaggtga tgaaggaaca
   129361 gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa aacatcctga tttggaaatc
   129421 gagatccacc aaggagatca gcctgtctat ccttatctat tttctgctga ataatttctg
   129481 tgtcagaata tctttctaac acagtattca ttcataaaag gccttcggtt agtgatcagg
   129541 tatgatcgct gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt atgcgttctt
   129601 tagatgatcc aatcactatg ctggcaggag tcggagaaaa aagagcagct agtcttgcca
   129661 gcttgggcat ccaaacgatt gaagacttac tgacttatta tccttttcgc tacgaagata
   129721 ttcaggaaag aaatctaaac gagatccagg atcaagaaaa ggttactttg aaaggaatcg
   129781 tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa aagccgattg caatttcgta
   129841 tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa
   129901 aagtcatttt atcggaggaa atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct
   129961 tgaacgggat gaagatttta ggttctcaaa caatggatga cttttcgccg atatatcacg
   130021 tcaacaaatc aattcgtcaa acgacattag ttgatctaat caaaaaaggg tttcaagaat
   130081 acggcacatt gattgaagaa aatctgccaa acaaccttgt ggagaaatat cgactgcttg
   130141 accgagcttc tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag
   130201 ccaaacgacg tgtcgttttt gaagagttct ttttatttca aatgcgtctt caaggattga
   130261 aaaaagcaga aaaagcagaa acgaatggtt tggagatcct ttatgatgtc caaaaattga
   130321 aacaatttac tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg
   130381 aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg acttttgcag ggagatgtag
   130441 gtagcggaaa aacagtggtt gccgcgattg cgttatacgc taccgttaca gcaggtttcc
   130501 aaggtgcgtt gatggtgcct acagaaatct tggcacaaca acatatggaa agcttgcaac
   130561 agctgtttga tcccaatgaa gtaaccatcg cattgattac ggggtcaacg aaagcaaaag
   130621 aacgacgtga gctccttgaa aaactagagc aaggggaaat taacgtcgtg atcggcacac
   130681 atgcattgat ccaagatgga gtcgaatttc agaatttagg tctagttatt acggatgagc
   130741 agcaccgttt tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg cgaccagatg
   130801 tcttatttat gacagcgact ccgattccta gaacattagc tattacagca tatggtgaga
   130861 tggatgtatc tatcattgat gaacttccag ctggacgtat cccgatcgaa acacgctggg
   130921 taagaacacc tcaattggac agtgtactgg attggacgaa aaaagaactg gcaagaggac
   130981 atcaaatgta tgtcatctgt ccgctgattg aagaatcaga agcattagat gtaaaaaatg
   131041 cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt
   131101 tacatggtaa aatgaagaat caggaaaaag aagtaatcat ggaagccttc aaagacaacc
   131161 agatgcagat attagtttcg actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga
   131221 ccgtcatgct gatcatggat gcggatcgat ttggattggc acagctgcac caacttcgtg
   131281 gacgtgttgg acgcggaagt gatgcttcct actgtatctt agttgctaat cctaaaaatg
   131341 aactaggtgt ggaacggatg aaaatcatga cagagaccac caatggtttt gtactaagcg
   131401 aaaaagatct ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc
   131461 ctcaatttgc gattgctgac ttggtgacgg atggaaatgt tttggaggta gccagagaag
   131521 aggctgcagc cctttggcaa ataaaggatt ggcagatgct tcctgagtat cagcctttag
   131581 cgcaaagcct ccaaatagaa aatcaggata atcgttattt cgattagcag actgtgctat
   131641 actttatact gatgacggaa aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg
   131701 gcggagatca cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag caagattttc
   131761 cagaagtaga attcttactt tacggaaaag aagcagaaat caagaaatac ttaacaaatg
   131821 aaaaaaacat cacgattatc catacagacg aaaaaatcaa tagtgatgat gagccagtaa
   131881 aagcaattcg acgaaaaaag actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg
   131941 gtgaagcaga tgctgtattt tcagcaggga atacaggcgc gctactagca gcagggttgt
   132001 tcatcgtagg acgtatcaaa aatatcgaac gccctggctt gatgtctacc ttgccagtag
   132061 tagggaaaga aggtgccggt ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac
   132121 cagaacattt gttgcaatac ggtattttag gttcttttta tgcacgtaaa gtccgaggta
   132181 tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag
   132241 tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag cttgaatttt attggaaacg
   132301 tagaagctcg tgagctactc aatggtgtgg ctgatgtagt ggtcacagat ggatttacag
   132361 gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa tatgatgaat ctattaaaat
   132421 ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta aaagacggct
   132481 tacgttcttt gaaagcagaa atggattatt ccaaacatgg cggtgccgtt ttgtttggct
   132541 tgaaagcacc agtcattaaa actcatggag caacaggccc cgatgctgtt cgctatacga
   132601 tccgccaaat ccacacaatg cttgagacag atgtagttgg acaattagtc gaacaatttg
   132661 aaaaaagcga agaatagtat ttggatatct gaagcgatcc gttccattga aaaaaaggtg
   132721 gacattcctg aaaaatgcca gtaaaattag ccatggtgta ggtaattgac aagtgtatga
   132781 ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc gaaaattatt
   132841 tcaaatcatt ttgagataga taccgataaa gtgacggatg aattgaacat caaagatgat
   132901 ttaaaagctg attcaattag tattatggaa ttcgttttag agcttgaaga tgaattcggt
   132961 acggaaattt cagatgaaga cgctgaacaa attgaaacag tcggtggcgc agtagattat
   133021 atcagcagcc atcttaaata atttcaaata aaagcaaaga aggataaaaa gctgcatgca
   133081 gctttttatc cttctttttt taatcttttt gacacgtaaa agtcattttt ctgataattc
   133141 tggcgataaa cagaaattat gttgcaaagt aaagcggttt tctatataat tattaataat
   133201 tcattacaca aagataagaa taacctttgt tataaaacga tttattctgt gtaatgtcct
   133261 tttttattaa gaaaaacttc catttaggaa gcagagaggg gtattcgatt gtccgataac
   133321 caattattag aagttcagaa cctacataca ggattccgac tcaaagatga ctattacgat
   133381 gcagtagatg acgtttcatt tactttagac aaaaacgaga ttttagcaat cgttggggaa
   133441 tctgggtgcg ggaaaagtac cttggctact acgattatgg gattacttga tcctaataac
   133501 acaaaaatta caggtgagat tttatataac gatctgaatt taatcgacct gaacgaaacg
   133561 ctctataata aaatccgcgg aaatgatatc ggaatgattt tccaagatcc gttgtctgca
   133621 ttaaatcctc tcatgagaat cgaagaccag atcaaagaag gactttccta tcacacaaag
   133681 atgaatgcag aacagcgtca agctcgtgca ctagaattgc tagaacaagt agggattcct
   133741 aatcctgcga gagttggacg ccaatatccc catgaactat caggcggtat gagacaacgt
   133801 gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg tcattgcaga tgaaccgacg
   133861 acagccctag atgtaaccat ccaagcacag attcttgatt tactgaaaga ccttcaggaa
   133921 gaaacaaaaa cagggatcat cttgatcact catgacttag gagtcgtagc agaaatggct
   133981 gatcgagtgg cagtgatgta tggcggacag ttcgttgaag tagcaggtgt aaaagaactg
   134041 tttgatcatc ctaagcatcc ttatacacgc tctttactga actctatacc acaagaagga
   134101 aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc cttctctgaa aaacatgcct
   134161 cgtaaaggtt gtcggtttgc accacgtatt ccatggatac cagcaagcga tcatgaagaa
   134221 gagcctatcc ttcatgaagt cgcaccgaac cacttggtac gttgctcctg ctataagcat
   134281 ttccacttta gagacgaaaa aggagaggtg tagatgacag aattaatcac aatcaaagac
   134341 ctgaaagttc attaccctat tcgcagtgga ttcttcaatc gagtaacgga tcatgtatat
   134401 gctgtagatg gtgtggattt tattatcgaa aaaggaaaga catatggttt agtgggagag
   134461 tctggctctg gaaaatctac tacgggaaaa gccgttgtgg gattagaaaa ggtaacagcc
   134521 ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa gcaaccgcaa aaaaatgggt
   134581 tataacaagg atgtgcagat gatcttccaa gattcaatgt ccagtttgaa tcctaaaaag
   134641 cgagtgttgg atatcattgc tgaacccatc cgaaattttg aacggttaag cgatcaagaa
   134701 gaaaagaaaa aggtaaaagg actgctagat atcgttggga tgccggaaga tgcgctatat
   134761 aaatatcctc atgaattttc aggcggacaa cgtcaacgtc taggagtagc ccgtgctgtt
   134821 gctaccaatc caaaactgat cgtagccgat gaacctgtat ctgctctgga tctatctgtt
   134881 caagcgcaag tattgaattt catgaaacga atccagcaag aatacggcct cagctattta
   134941 ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg acaatatcgc aattatgtac
   135001 aaaggtcgat ttgtagaaat cggtacaaga gaagacattt ataatgaccc gcgacatatc
   135061 tatacgaaac gtctgctttc agctattccg caaatcgatg tagaaaatag ggaaagccac
   135121 aaagaaaatc gacgaagagt agaacaagag tatatccaaa accaaaaaga atactatgat
   135181 gcgactggtc gagtctacga tttgcgtacg atcacaacta cacacaaagt ggcgttgaaa
   135241 gatggaggtg caagctaatg tggaaaacga ttctgcgacg tgttttattg atgatcccgc
   135301 aagttattat tttaagtgtt ctgatcttct tattagccca agcgatgccg ggggatccat
   135361 ttaccggttt gatcaatcca aatcaggatc cggcagtgat cgaacagatg cgacaagcag
   135421 ctggcttgaa tgacccatgg tatgtccagt acttccgttg gatcggaaat gctttgcaag
   135481 gcgattttgg tcaaagtttc ttatacaaac gctctgttgc tgacttgatc ggcgaacgga
   135541 tcatcaacac tttatatcta tcgatcttga cagttgtcct tacttatttg attgctgtgc
   135601 ctctggggat gcttgctggc cggtatcaaa attcaatctt ggataagatt gtcgtaatct
   135661 acaacttctt tagttttgct gttcctttat ttatcttcgg cttgatcatg ctatttgttt
   135721 ttggttatcg tttaggctgg ttcccaacaa gtggatcaca aacactaggt ttgcaaggtg
   135781 gatttatcac tcaatggata gaccgtttga aatatattct gttaccatca attacccaag
   135841 catttttggc gactgccgtc acaatccaat atctgcgaag tgaagtaatc gattcgaaat
   135901 cgctcgattt tgtcagaaca gcccgttcca aaggggtacc gacgaataaa gtattcagtc
   135961 gacatatttt ccgaaatgca gcgttgccga tggcttctca aatggggtat gaaatcactt
   136021 cgttgattgc tggttctgta gttatcgaaa agatttttgg ttatccggga gtcggaaaac
   136081 tatttattga ttccatcggc caacgggact ataccgtgat cacatcctta gtattgattt
   136141 taggaatcgc gacgctagtg gggacattac tttcagatat catcatgagt atcgttgacc
   136201 cgcgtattcg gattcaataa aaccaatcag aaaggagaat ggatcgatga gtgaaaaaaa
   136261 caatacagca gttcaagaaa gtattcctcc tatggggata cgaatgattg ctcgagaatt
   136321 taaaaaagat aaaattgcta ttttttcatt aggtttgcta gtggttttat tattagtgat
   136381 ctttatcggt gcgatgctga ccgatcaaga caaagtgatg tatgttagta tcttcgacaa
   136441 atacgctgca ccaggaacag tttcgacaca aggacaaaag ttcattctgg gagcagatga
   136501 aggcggacgt gatgtattag gacaattgat cataggtgct agaaactctg ttacgatcgg
   136561 atttgcgatc acgatcctta cttcgatcat cggggttgga ttagggatca tctctggcta
   136621 ctacggaggg atcattgaca acatcttgat gcggatcgtt gattttatca tgattctacc
   136681 gatcatgctg atcattatcg tcttcgtatc gattatttcc agatacaatg tctggtcctt
   136741 tatattcatc atgagtgcat tttattgggt agctaaagcc cgattattcc gaagcaagac
   136801 attatctgaa gcaagaagag attatgtcag tgcctcaaaa acaatgggaa ccaatgattt
   136861 taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca ttaatcatta ctaatttgac
   136921 gatgaacttt gcggcaaaca tcggtatcga gacaactttg actttcttag gtttcggctt
   136981 gccggcaaat gtacctagtt taggtacatt gatcggttat gcaagtaacg gagaagtctt
   137041 agtgaatcgg caatggatct ggttacccgc atcaatcctt attttagttt tgatgttgag
   137101 tatcaattat gttggacaag cattcaagcg ttctgcagat gcgcgccaac ggttaggcta
   137161 gtatagaagc gaagggggaa ataggaatga agaaaacgat ttttggattt gcagcgctga
   137221 tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac agatacctct ggaaacaact
   137281 ctggaggcaa cggcaataac aaagaggaat taacttttcc aattgcaaca aaaaataatg
   137341 atgatgcaat cgaagacggc gaattagaag ctgcggtcgc aacggacagt cagtttaaag
   137401 gattgttcca gtgggagttt taccaagatt catatgatgc agcttttatg gctccttctc
   137461 atgaaccgct atttgcaaac gatgacgatt tcagaatcac agacgacgga gctgcaacgc
   137521 ttgatctaga tgaagatacg aaaaaagcga caatcaaagt aaaagacaac gtcaaatggt
   137581 cagatggtga agatgtaacg gcggacgatg tgattttccc ttacgaaatc attgggaata
   137641 aagattacac tggtatccgt tatgacgaca ctttccgtaa catcgtaggt atggaagaat
   137701 atcatgatgg aagtgccgac acaatctccg gaatcaaaaa aatagacgac aaaacagttg
   137761 aaatcgaata taaagaagta aacccaagca tgcttcaatc gggtggtggt atctggtcat
   137821 atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa agatatggaa tcaagcgatc
   137881 aagtacgtaa aaatccagtg acttttggcc cttattacat gagtaatatc gttgcaggag
   137941 aatcagtaga atacttgcca aacgagcact actggaatgg tacgcctaaa ttgaaaaaga
   138001 tcacgatgaa atctgtaccg atggcaagtg caactgaagc attgaactct aaattatatg
   138061 atatgatttt ccaaatgcca acagatactt acgataccta taaagaaatt gctggctata
   138121 caaacttagg ccgtgaacaa acttcttata cttatctagg attcaagtta ggtaaatgga
   138181 atgcagatac aaagagtgtg gaatacaatc cagattcaaa aatggctgat aaatcattac
   138241 gtcaagcaat gggctatgca ttagataata atgcagtagg tgaacgtttc tatgcaggtt
   138301 tgagaagcaa tgcgacttca ttgattcctc cagtattcca gtcataccat gatgatgaga
   138361 tgaaaggatt cacacaagat ctagacaaag cgaaaaaact attagatgat gctggctaca
   138421 aagatgtgga tgacgatggt ttaagagaag acaaagatgg aaataaacta acaatcaact
   138481 ttgcttcgat gtccggtggg gaaacagcgc aaccattggc agattactat atccaacaat
   138541 ggaaagaaat tggtttggat gtccaataca caacaggtcg tctgatcgaa ttcaattctt
   138601 tctatgataa attagaaaac gatgatcctg aaattgatgt ttaccaagct gcttggaata
   138661 caggaactga tccaaatcca agtggactat ggggaccaaa tgctgcgttc aactacactc
   138721 gttatgaatc agaagaaaat acaaaattga tcaacgatat cagttcaagc aaagcatttg
   138781 atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca atatgcattt gaagaagcat
   138841 ttgcgattcc aacgttgttc cgtaatgaag tattgccagt caacgaccgt gtcaaagact
   138901 gggattggtc ttatgatgta ccaaacccat gggcaaatgt ttctgttaca gcagattcac
   138961 gttcataatc actaaaaatg accgaaaaag ggcgatgtcc tttttcggtt ttttgatatt
   139021 tttctgttta tttctctaat attcaggtca aggcgctgat ttcatgactt ctccaataaa
   139081 cggtgttcaa agtccagttt ctcggtatta agtcaaattt atgcaaaaat atggaaagcc
   139141 attttcacaa aattttttct tcatactcga aactaaacga atttttcaca acttctcgtc
   139201 aatcactagg attattaaag ctttacttac cttttgaccg aaccatttca aatttgacaa
   139261 aaaaccagta taataaggaa gtatcgaaaa aatatccata aaaggaatgt ggaaaatgga
   139321 caaccagctg acaaaagaac taaaagaaaa ctataacatc gtttttcacg atctaaattt
   139381 gcttgaacaa gcttttaccc attcatccta tgtgaatgag catcgcaatc tgcaattatc
   139441 ggataatgag cgacttgaat ttttaggaga tgccgtttta gaactgatgg tttctcagta
   139501 tttattccgt ttatatcctc atttgccaga aggaaaattg acaaaaacac gagctgcgat
   139561 cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt cactttgatc aatatgtttt
   139621 gttgggcaag ggagaagaga attcaggagg acgtacgcga ccagcattgc tttgcgacct
   139681 attcgaagct tttttaggcg cgttatatct tgaccaggga ttcgacacga cccatgcatt
   139741 catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct ttttcacatg agatggatca
   139801 taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat gtatcaatcg aataccgatt
   139861 gatcaatgaa gaaggtccag ctcatgaacg agtattttgg atcgaagttt atgtagatga
   139921 tcaattgatt ggcactggac aagggaaatc gaaaaaatta gcagaacaag ctgcggcgga
   139981 aaatgcattg gcagcactgt aaaagtaagt gagggaaagc tttgtatttg aaacgaattg
   140041 aaatagccgg ttttaaatca tttgcagaca agacagtcat tgattttgaa aatagcgtca
   140101 cagcagtggt tggaccaaat ggcagcggaa aaagcaatat tacagaggct atccgctggg
   140161 tattaggaga acaatctgct aaaagtctgc gaggcggaaa aatgccggat atcatctttg
   140221 ccggttccga tacccgtaag cagctgaata ttgcagaagt cacagtgatt ttagacaata
   140281 gtgatcatta tctgccattg gagtacaatg agatcagtgt aacccgccgt tatcgacgga
   140341 ctggggaaag cgagtttttc atcaataaac aaagctgcag attaaaagat atccaagaat
   140401 tgttcttaga ttctggatta ggaaaagaat cgttctcaat catttctcaa ggaaaagtag
   140461 aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat ctttgaagaa gcagctggtg
   140521 tcttgaaata caagcagcgg aaaaagaaag cagaacagaa gctttttgaa acagaagaca
   140581 acctcagccg cgtccaagac atcatccacg aattggaaga acagttaact cctttggccg
   140641 cccagagtga ggcagcaaag gagtttttac ggctaaaaga aacactgact caaaccgatg
   140701 tctctttgat ggttgctgaa atcaagacag caaaaaaaga ttgggacaac aaacaagcac
   140761 agcttgcgaa attcaatcta gaattaggca aactatctga gtcgatccaa gaacaagaat
   140821 ccattttggc gaagcagcgt aaagaaaatg cgcaagccga tcgtctgatc gaaaaaaatc
   140881 agcaagtatt attagatctt tcggaaaaac tgaagcagac agaaggacaa aaagatgttt
   140941 tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga atatcaaact tcattagcag
   141001 aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga aagcttgatg aaagcagcag
   141061 cagaaaaaga aacagagatc caaaaagctg aagcaaatct cataaaaaca cagcaagaat
   141121 tagaaaaata ccaaaaatca acgaaagaat tattggcaga attaagagat caatatgttg
   141181 atttgatgca agaacaagca gctgtaggaa acgaactgaa gtatctagaa cgtcaatata
   141241 tacaagaaac agcaaaaagc aagcagacgt tagccaagca atcggaagta gaagcatcag
   141301 tagaccgatt gatcttacaa aaagaagaat tgacacaaaa gcaggcgcag ctaaaatcat
   141361 ctttaactga aacaaaagag aagttagaaa tgatccaaca aaacgggaaa aagttccaag
   141421 aagcattagc aaaagaacag cctaaaatgt atcaattaat gaatcaagtc cagcagctac
   141481 gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta ttttggtttt taccaagggg
   141541 ttcgattggt cttgcagcat aaacagcagc tttcgggtat tgtcggagca gtggctgaac
   141601 tgatcgatgt tcctgcagat ttcacgttag ctatcgaaac agctttaggt ggggcagccc
   141661 agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc catcacgtat ttgaaacagc
   141721 aaagaggagg aagagcaaca ttccttccac tgacgacaat caaaccaaga caattaccgg
   141781 ctcatatatt gacacaagca gcagctgtag aagggtttat agggattgcc agtgaacaag
   141841 tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct gctaggaacg atccttttag
   141901 ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat ccgctatcaa tatcgagtgg
   141961 tttccttaga aggtgatgtg atgaatgccg gaggatccat gacaggtgga gcgaataaac
   142021 gaggaaacca aggcagtttg tttgtacaaa atcaagaatt gaaacagttg actagtgaat
   142081 ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa agttcaggag ctgcaacaag
   142141 aaacagctcg tttagccgaa gaacaagaag ttttgcgtac acgaggcgaa caattacgtt
   142201 ttgaagaaca agaagcgaca aatcagctac aaaacatcat aaatgaactt gaacgttttg
   142261 aaaaagaaaa gcaaatctca acttttgaga cacgtgaact gcagcagttt atcgaagatt
   142321 acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga tcttgaatcg caacgacaaa
   142381 aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga ccagatggaa gccagaagag
   142441 cacaagtaca aagtcaaaaa gcacaagaac aagcagatct tgctgtcttg aaagagcagt
   142501 ttaatcatct gcagatccag ctccgcggtg cccgagtcca aaaagcagag gcaaccgaac
   142561 gtcaagaagc gatagaaaag cagttggcga cattgacagc agacttttct gatcatgaag
   142621 tcacagaaga aagtttggaa aaacaaatta acgaattatc tgctcagcga gaaacattaa
   142681 aagccgagtt ggcaaaagcc aaagagcagc gagatagaag gcaaaaggag atcgataagc
   142741 ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc acgactgtcg gaacaatcca
   142801 aattagaagt ccaaaaagac cgagctgaaa tgattttaga taatcatttg acctatctgc
   142861 agtcagaata tcagctgaca tttgagaaag cttcccaaga ctatcaagaa acaaccgata
   142921 tcgaagacag tagaacaaag gtttcatcct taaaagaaca gattgaaaaa ctgggaccag
   142981 taaacttaaa ttcaatcgaa caatacgaac aagtcagtga aagacataca ttcttagcaa
   143041 cacagcgaga tgatctgtta gcagcgaaaa accaattgtt tgaaacgatg gatgagatgg
   143101 atgatgaagt gcggacaaga ttcaaagaag tatttgaagc tatccgtcaa gaattcaaaa
   143161 ttgtctttcc aaatatgttt ggcggcggta aagctgaatt agtactgacg gaaccaactg
   143221 atttgctgaa aacaggtatt gagattgaag tacaacctcc tggcaaaaaa ctgcaaagtc
   143281 taagtttgct ttctggagga gaacgagcac ttacagccat cgcgttgctc ttttccatta
   143341 tccgtgtctg tcctgttccg ttttgtatct tagatgaggt agaagcagcg ttagatgaag
   143401 ccaacgtgaa acgttttggt cgttacttga gcgatttcca agatgacaca caatttatcg
   143461 tagtcacaca ccgcaaagga acgatggtcg cagcagatgt actgtacgga gtgacgatgc
   143521 aggaatccgg tgtatcaaaa atcgtttctg tacggatgga agatatcaat gaagaggcga
   143581 aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa ttagtagcaa tcgatttgga
   143641 cggtacatta ttaaatgaac aaaagctgat ttcagatgag aataaacaag cattagcaca
   143701 agcaaaacag caaggggtca agatcgttct atgtacagga cgccccttag ctgcgatggc
   143761 gcattatctg caagaattag gactggtaga tgaaggtgac tacagcatca ctttcaacgg
   143821 cggtttggta caaaaaaatg atactggtga aatcatggaa aagaaagtga tggaagtatc
   143881 agatatccaa cgcttataca aactggctca gcaattggat ctgccactag atgttttgtc
   143941 tgacaatgtg gttttacagc tgcctagtgc cccaaggaag caatccttgt acaatgtgtt
   144001 gaataagctg ctgcaattcc aaccagcgga tataagcgat attacagatg aatttgtgct
   144061 taataaggca gtaatcgctt atgaccaaga agaattagat cctaaaatca aagaaatacc
   144121 agcagaattc catgaaatgt atgagatcat caaaacaaga agtatgcttt tggaatttat
   144181 gccaaaaggt gttacaaaag cttatggtat ttctttattg gcaaaagatc ttggtttgaa
   144241 acaagaagaa atcatggcga tcggtgatga ggaaaatgat ttaccgatga tccaatatgc
   144301 tggactaggc gtagcgatgg agaatgctgt gcctttcgtc aaagaagcag cagatttcgt
   144361 gacagccagc aatgaagaaa atggcgtagc caaagtgatc caaaaatttg tattagagta
   144421 gggggagtca aatgggattt tttgataaaa tcaagcgtgc ttttactggc gaagagaaaa
   144481 cagaagaagt tgaagtacag gagaagtacg acaagggttt agaaaagacg cgtaaaacat
   144541 ttggtgaacg gatgaatgag ttgtttgcca attttcgaac agtcgatgaa gacttttttg
   144601 aagaattaga agaaacactt atcggtgcag atgtaggttt tgaaacagcg atacgaatta
   144661 ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa aaaatcggca gaagtccaaa
   144721 atgcgatcat cgagaaactt gtggatctct atgaagcgga aggaatcaat gaagtaaatg
   144781 aactaaatat ccaagaagac ggactaacag ttattttgtt tgtcggtgtt aatggtgtag
   144841 ggaaaacaac aagtattggt aaacttgccc atcagttcaa aaacgaaggt aaaaaagtat
   144901 tgatggcagc agcagatacc ttccgcgctg gagcaatcga ccagttagtt gtctggggcg
   144961 aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg cgatccagca gctgtcgtat
   145021 tcgatgcttt ggaaagagca aagacagagc aggcagatgt tttactagtt gatactgcag
   145081 gccgactgca aaataaagtg aacttgatga acgaattaga aaaaataaaa cgagtaatcc
   145141 agcgggaatt accagaagca ccccaagaaa cacttcttgt attagatgca accactggtc
   145201 aaaacgcgat ggttcaagca aaacaattca aagaaacaac agatgtgacc ggtcttgttt
   145261 tgacaaaatt agatggtaca gcaaagggcg gtatcgtact tgctatccgt aatgaattgc
   145321 atttaccagt gaaattggtc ggtcttggtg aaggaatcga tgacttggag ccgtttgatc
   145381 caaatgattt tgtcgttgga ttgttcaaag gattactaaa agaagaataa cccatgtcta
   145441 aaaagcagga gctgtgacaa aagttgcagc tccttgtttg aatgattgtt tttaacatag
   145501 ctgtgttttt gatgccttca tttttatact tatttgtaag cggttctcaa acaatttcgt
   145561 tctttttctt agactttttg attataatta gattcatatg aattggaggt cacttaatga
   145621 tgaattttaa gcaattatta ttgcatgtca atgcgcgccc ttttatcgac caagctcgat
   145681 ttggtataga acgggaagga cagcgggttg atcttgcagg aaatctagca aaaaccgatc
   145741 atccagcaat ctttggcgat cgatcctatc acccctatat ccaaacagat tttagcgaaa
   145801 cacaaacaga gatgatcacc cctgttaccg attctattcc cgaattattt cagtatctcg
   145861 ctgctgttta tgatgtgact gctcgttcta taccgaaaga agagatgatc tggccattaa
   145921 gtatgccacc tgccttaccg gaaaaagacg aagagatcat tattgcaaaa ttaaaaaatt
   145981 tcgaagatgt cttgtatcga cgttatttag caaaagaata cgggaagcga aaacagatgg
   146041 taagcggtat tcatttcaat tttgaatttg gcgatgagtt gctaagaaca ttgttcagcc
   146101 atcaggaaga atttcaagat ttttccgaat tcaaaacaga actttatttg aaaacagcga
   146161 gaaactttat gcgctatcgc tggatgatca cttatttatt cggtgcttca ccaatgagtg
   146221 agaagaacta ctttttagat gaatcacatc cgcaagaacc tgttcgcagt attcgcaaca
   146281 gtgcgctggg ctatacgaat catccaaatg tgaaagtttc ctatgcttct atgaaacagt
   146341 atttagcaga tatcgagcga atgatcgaag aagggaaact ttcggaggag aaagaatttt
   146401 atacaccgct tcgtttccga ggcggaaaga aagtcgcaga tttagcaaca acaggtgttc
   146461 gttatattga attgcggaat atcgatttga atccttatgc aagattgggt atcaatccag
   146521 agcaagttcg gttcttacaa ttattcctga tgtacatgtt atggacagaa gaaaaagaag
   146581 actgtgacca atgggtagca gaaggtacga ctcgaaataa caaagtagca ctagaacaac
   146641 catcagatca aacagaattt catcaagaag gtagagaaat tcttgaagga atgaagcaaa
   146701 tgctggttga attggattgg ttggattctc tttatttagt agaggaagca ttgactcaaa
   146761 tggatcatcc tgaacaaacg ttagcagcga aactctatca agaagcacag ctatcgagtc
   146821 agcaagaagt tgctgtggca ttgggacatc aatattataa agaaagccat gaacgtccct
   146881 atcagttggc cggttttcgc gaaatggaac tttctactca gatatttatg ttcgatgcca
   146941 tccaaaaagg tgtccaagtc aaagtattag atgagtcgga tcaatttttg cgtctgcaat
   147001 tccaagacca tgtagaatat gtgaaaaatg caaacatgac aagtaaggac agctatatcg
   147061 tcccacttat catggaaaac aaaacagtaa cgaaaaaagt cttaaaagaa gctgggttcc
   147121 gggtaccagg aggcgcagaa ttttcatcca tggaagaagc ggtaaaagct tatccgagat
   147181 ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa ttacgggtta ggtatcacga
   147241 ttttcaagga aggagctagt ctggaagatt atcaagcagg tttagccatt gcttttcgtg
   147301 aagacagttc ggttttagta gaagaattta tgccgggaac ggaatatcgt ttctttgtga
   147361 tcgacggaga agtccaagcc atcatgttgc gagtccctgc taatgttatt ggtgacagta
   147421 tccgaacagt gaaagaactt gttgaagaaa aaaatagtga ccctttgcgt ggaacgaatc
   147481 atcgtgcacc tttagaattg atccaattag gtgaattaga acaattgatg ttgaaagaac
   147541 aagggttgac gatcgaatca gttccccaag ccaatcaaat tgtttatcta agggagaact
   147601 caaatatcag tacaggtggt gattcgatcg atatgactga tgaattttct gaagcttaca
   147661 aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa gattagtggt atcgatttga
   147721 ttataccaga taaagaaatc gatcctacaa ctgataaaaa agcatatggg atcatcgaag
   147781 caaatttcaa tccagccatg catatgcatg tttatccttt tgcaggaaaa ggcagaagat
   147841 tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt ttaaaagaaa aagcttttga
   147901 cagattgtcc tatttgatgt caatctgatc aaaagctttt tttgcatgta aaaatttatg
   147961 tgttttttcg ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt
   148021 ttcagctggc gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt
   148081 tactgcttct tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc
   148141 gttgactaca gtgaaacggc tattgctttt cgttgctagc tggatatatt ggttttggac
   148201 agaaaggggt agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt
   148261 taaaaagttt tctttgtttt ttggttggat catttcagca acagtcatac taagatagca
   148321 gcgttccctg aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc
   148381 ataaagtccc ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct
   148441 tcttctcttt gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa
   148501 aaagcggttg cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta
   148561 gaaaggtgga ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta
   148621 tttcattgtt catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc
   148681 aaagcttaca aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg
   148741 gagcgaccag tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt
   148801 caccaacgac tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt
   148861 tcctacctgg tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag
   148921 gaacattgct ttttttatcc cttacgcgat tgttcctcca gaaaaatcaa ttgatgcttc
   148981 cgttgattgg agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc
   149041 aattccagtt ggtacaaaat atgacttcat ggcttcaagg gaattttgcg actgtgatac
   149101 gaggaaatta tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg
   149161 cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc agtaaatctg ggaatagatt
   149221 accagaagat gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga
   149281 tcatggtagg aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat
   149341 atggagatca agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag
   149401 tattttgcga tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga
   149461 tcgttggaat catcggtggg gctatcttcc tttatcttct gataagggga cgacgatatg
   149521 cttaaaaaaa agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga
   149581 atgtacttga cgatccatac atatggaaac tggcattttg catgtgcatt gaggagtaag
   149641 aaaatttttg catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag
   149701 acaattacgc ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc
   149761 cttgtccaaa cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt
   149821 acagtttttt tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc
   149881 tttcttttga aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta
   149941 ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat
   150001 gatttgcttc aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg
   150061 cctgcttctt tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta
   150121 gacgttttcc atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt
   150181 gaatttttga ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt
   150241 ccagttactt ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat
   150301 cgtcatggtc agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga
   150361 cagctcgcag tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt
   150421 gtcggaggaa tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg
   150481 atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa
   150541 cgattcctga aaagaaattg actgctttta tcggtccaaa tggtgctgga aagtcaacgg
   150601 tgttgtcgat gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata
   150661 acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat
   150721 cgaatgaaat aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt
   150781 atacgaaagg aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacacc
   150841 ttgggttaga agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac
   150901 gtgcgtatat tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt
   150961 tgaacaatct agatatgaat tttgcggttc aaatcatgca gattttaaaa aatttagtac
   151021 aagaaatcgg gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg
   151081 cagacgaaat tgttgcaatg aaagatggac agttatttac gcaaggaacg acccaagaaa
   151141 tgataaaaaa agatgtattg gatgaattat acgaaatgga catacggatt tgtgagattg
   151201 aaggccgcag attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa
   151261 ttagtagtag gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagcaac
   151321 aacaccagtc aggcagacac aaaaaaaagc agcacacaaa ctgaaactac attgacgata
   151381 acagatagta atggagacca aatcgaggtg ccaaataatc cagaaaaagt cgtcgtattc
   151441 gacaatggtt ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca
   151501 gctacaagca gccttcctga gtatctttca tcctataaaa aagtagaatc agcaggtgga
   151561 ataaaagaac cagacttaga aaaaattaat cagcttcagc cagatctaat catcatttct
   151621 ggccgacaaa gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct
   151681 ttagatgcga aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc
   151741 tttggtaagc aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag
   151801 acgaaaaaga aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg
   151861 caattatcag cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt
   151921 gaacaagcag atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat
   151981 gtgctggaaa aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga
   152041 gacgacagca aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa
   152101 aatcagcaga tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa
   152161 tcaatgaaac tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg
   152221 attaaactct catactatgt aacaataatg tttcttacat tcttaaatag caagagccta
   152281 tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt
   152341 tttatttatt tgagaaatct tttttcaacg tgaattcatt atctacatca tacgggttga
   152401 ttctagaatg gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct
   152461 cctttgattt gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt
   152521 ttattttgta ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt
   152581 ccactagact agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca
   152641 aatcgcagtt gtcacaatct cttctaaacg atgttccatc tgctgcttct cggcaataag
   152701 cccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat tactcaaagc
   152761 aaatcgcagt tgtcacaatc tcttctaaac aatgttcaat cagctgacct tcggtattag
   152821 ctcaaagcac tgatttcaca acctctattt aggcatgtgt ctcagcaagc ggttgatagt
   152881 tgatgatctg catttttcga taaaaatcgg cgggaagctt actccaccgt tttaataatt
   152941 gatagaaata actctcttct gctttgattt tatactcgct taatatcaat ccaaaactat
   153001 tcgttttttt tgaagctaat tttttataaa tatccaatgc tttggtttta tcttcattga
   153061 caaagagtgt ttttacttca atataagaac cctcgatatt ttgtgtttct tcgataaaat
   153121 agatgttttt tatatattcc ttcatagtga ttcctccaaa gaagtctttt atttgataag
   153181 tttactctac cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag
   153241 ttgttaaagg ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta
   153301 aacaaataac aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg
   153361 gaaagttgtc tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata
   153421 aattgagaac agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct
   153481 atgacatgag gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa
   153541 gaataaatta gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt
   153601 tgcagaaagc caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa
   153661 agatacacaa acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat
   153721 caaaatcaaa gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc
   153781 aacttataat agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt
   153841 tgttcgaaaa gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt
   153901 ggacatcaat aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca
   153961 caagatcact cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc
   154021 tgttgtcgaa tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga
   154081 gttggcagat gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa
   154141 tgaacggtat attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga
   154201 ctatcagttt tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg
   154261 gtatgatcgg ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc
   154321 agctgcaaaa aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac
   154381 tcatttcgtt cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa
   154441 caaattaaat gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa
   154501 tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca
   154561 cacgatttac agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt
   154621 tgtcttgaaa gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg
   154681 tgcccatcac ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca
   154741 agcgatgaca atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg
   154801 taaaacataa ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt
   154861 gcggaaaata atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag
   154921 atgcaagcgt gtattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt
   154981 ttatttaatt ataattaata ttgattgaag tctaggcagt caagtcacat tttgatagat
   155041 aggaggggga cttacgaatt attttaaagg ctatgacagc tgtatttcta gattgtgttt
   155101 gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg ataatcattt ataactaatc
   155161 gatacatagg accaaatata ggtctatgtt cttttatttc ataagtatcg taaaacagaa
   155221 ttttgctatg gattaatcca atcgactgat atgtttagta aataaagaaa gaggaatgtc
   155281 aatgatgaaa aaagaaatcg tattaacagc agcaatgatg ttattcagca tggtagcaag
   155341 tacgactttt gtttctgcaa ctgaagtata cccaaaggaa tataacacag aaggaacgat
   155401 tacatttgaa gcaggagatg agggtgtaac tcctcctgtt gatccagaga atcctgatcc
   155461 caataaacct gtcgatccat cagatcctcc aagccctgga acaggagggg cattatcgat
   155521 tgattatgga tcaaaattca aatttgggac tcaaaaaatt tctactgcgg ataaaacgta
   155581 ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa ccgacatatg tccaagtgac
   155641 agataggaga agtaccttat ctgggtggaa attaagtgtc tctcaaccag aacagtttaa
   155701 aactgccagt ggggatgaac tagttggagc acagctgaaa tttacgaaag ggcaggctgt
   155761 ttctttggtt gatccaacat atacgccgca aacggtgaat tctgaattaa cgttgacacc
   155821 gggaggaaac aatacgttag caattaatgc taaatcagga acaggtgtgg ggacttgggt
   155881 ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg gtacagcttt cggtacccgg
   155941 taaatctgtc aagttagcgc aacaatattc cactaaatta gtttggacat tagaagatac
   156001 acctaacaac tagtttactg aaaagaactg ttaaaaataa atacgtagtc atattgtctt
   156061 gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa tttgtttgga tacgaatagt
   156121 tcatctagga atttaacgat caggaggaaa agatatattg aaaaagagga ggatcgtgct
   156181 cattttaagc agtctcttag tgactaatat ctttccgatc atagggtact ctttggaatt
   156241 gaatcagttg gagcatggaa atagaagaga cgaagaaagc aaactcactt cgacattcga
   156301 cagtttaata gaatccaacc agacacaaga aagcagtgag gatttaagta tttcgagtag
   156361 tggagagacc actgacaacg tagaagctga gggttcggac aaacaaggac tagaagatgc
   156421 accaatttct gcggttgagc tgcctgacga tcccaatatt atttctatcg ataaaatttt
   156481 tcaaacacct atcggtacta gcacgtccat attggaaaat gggaagttac tgcagttgaa
   156541 cccagcagaa aggtctcaaa gaggagctat ttggtcaaat aagaagatta gtttactctc
   156601 ggattttaca tttaaatctt atttatactt aggaaatcaa cgagatagag cgggagatgg
   156661 catgaccttt acattgactg gcgatcctag aatggctagt aatcccagtc aagttattgg
   156721 atcgccaggt atgggaattg gctcatattc tactcgttct ggccaacctt acattgcgaa
   156781 tgcgctctcg attgagtttg atacgtataa aaatagtggt tcttctgatc gaatggatcg
   156841 agagataact gctgataata gagggcatgg acatgtcgct tttgtcacgc ctaaaccaaa
   156901 taataataat tattctggag agcatactgg cgtgacggta gctcctacat ttctttctaa
   156961 tggcacatgg cgcttgttaa taattaaatg ggacgcggag caaagaagtc ttacttatga
   157021 tttggaaggt gtcggtcgct cttctcggat tatacctgat ctcattaggc agtttggttc
   157081 tacagaagta aattgggggt ttacttcttc aacaggtact ttttttcaag aaaatgctgt
   157141 cgctgtcacg cgacttccat cgaatgtcac atcaacagct gatgtaaaag tcaacgatag
   157201 tgattatggg gccatcgctg aagctggagg aaacgataag ataactatca gaaatacatt
   157261 gaaggtcgag ggggattttc ttgaatataa gaaaatgcag actagtatcg acctcccaca
   157321 agaattaact attcccgagc atatcttgat caatggaaat caaattgatg ccaaagatat
   157381 cttgataacc ggaaatcgag tcacgctaaa tctgacagat tattttattg ctggagatac
   157441 gttaataatc gaattggaga caactttgca ggaagtaatt cctgaaaaag tactcactat
   157501 gcattttgaa taccttgaaa acgggaattt gattgagcaa tcgaatgaaa taaacctaaa
   157561 gatcgcaaag ctgaaggaga tagatatcca tgtttattat aaagataaag acagtcagat
   157621 tgatattgct gaaatgaaaa caatcactgg gaaaatagga gagaagtata aagaaactcc
   157681 tattgatatt gaagggtatg tttttgactc ggattcagcg aatacagaag gcatcttctc
   157741 cgaaaataca gaggatatct atttttatta cagaaaaggc aaactttatc tctcaactgc
   157801 tcctcaatat gtcaattttg gaaaacataa aatatccagt agaccattaa gtgaattcgg
   157861 acagttttca ggtgggttga aaattataga tgaacgggct tttggaagtt ggaaattaca
   157921 attgaaacaa gagaaagttc ttacaaatgg agattttgag cttccagaag ctctttcctt
   157981 tgttacctct gaaaatacaa cggttatagg gacatccgct gttacgattt tcgaaagtga
   158041 tcaaaagggt gaaagcgatt tgtcctctct tttagattct acagctcatc gtggaatcag
   158101 aatagatatt cctgtagaaa accaaagaat cggaaccttt gaggggaaac tatcttggat
   158161 acttgcagat gtcccaggaa attaacagga agagaagaat ataacaaaat tattgagttt
   158221 caagaagggc acaggtacca taggctcttg tgtcccacag agacaagatt ttgttatagt
   158281 cttctcttac atatgattaa aggagcgatt ttcttgagac caaaacaaca attatttata
   158341 acactcctag ttattttagg ggcacttttt aacaatatat cagtgtcagc tagtgcattt
   158401 aatttttcag tgacgccgat aacttcagag aatcaaatcg acaagagaaa aacttacttc
   158461 gatcttcaac ttgatccgga tcaggaagta gaagtaaaag cggaattgag aaatgataca
   158521 gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa ccaccaattc aaatgtgatg
   158581 gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc tgatctttga tctggtagat
   158641 tatgtgagct atccacagat agtgacgcta aaacctaaaa gtgtccaaac cgttgctttt
   158701 catgtcagga tgccgaatga gcgatttgat ggtgtattgg ctggtgggat cacattccaa
   158761 gaaatccaga cggaaaagga ccaaacagaa actaaggatc aaagcttatc gattgagaat
   158821 gaatacacct atattattgc tttgttgatg caacagacac tgaacgaagt tgctccgaac
   158881 cttactcttc atgaagttaa accagatcaa atcaatgcaa gaaatgtcat cttagcaaac
   158941 gttcaaaatg atcaaaaaac ctatatcaat caagtagtta ttgaaacaaa aattacaaaa
   159001 aaaggacatt ctgaagtttt atatcaagag gaaaaagaag gattgcagat tgcgccgaat
   159061 acaaattttt cttttccaac tgctttaaat ggtcaaccgc taaccccagg tgaatatcac
   159121 cttacaatga ctgttttagg aaatgaaaat gaaaatggaa aattttcgag aaaaaaagga
   159181 aatactacga tcaactatac caatcaatgg gtatttgaaa aagattttac aatagacgga
   159241 aaagtagcca aggaattgaa cacgacggat gttacgatta aaaaagacaa taattggatt
   159301 tatatcttag tagcttcact tttcttattg tttattttat tatgcatact tttgtttgtt
   159361 ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata acaaatagta aatttttatt
   159421 attgtaggat ggttgggctc tttttataac aatataaaat ataccatttt attaataaaa
   159481 atgcatacta gtaaatgata taattctaat aacaggtttg atcgcgagaa aggccaaata
   159541 aaacgctctc caaaatgtta aaaaaaagac aggacgatgt atattataca aacaagaatc
   159601 gctgacaaca gtgactcgga tccaggaaat cagttttatc cccagctagt tggtgaatgc
   159661 atggaaagta atatacgtag aaagagctga tgactaatga attatgagtt ttttttaagc
   159721 aaagaagatc acagaagtta ttcattactt aaatatttag aagcaagctc aaatttatca
   159781 gagtcaatat cgaatgttca agaagagttg tcgctatcga catttctctt gaaaaaaacg
   159841 atcgataagc tgaaacatga tttacaaaag ttcgatctag agaaaaattt gcgagtaacc
   159901 gtcagtgaat tggatatatg tctggaaatt aatggaaaat gttctagcaa agctctgtta
   159961 tctcgttata taacggattc tttatctatg aaaatggtat tggctttctt tcaggaaaaa
   160021 tatatctcag tagaaaattt tgctaaacag catcacgtga gttattctgt ggcttataaa
   160081 gtattacaaa gactaaaaag aaatctaaaa aaatatcaaa ttttttttga aaagagaaag
   160141 ttg
//
LOCUS       DAFBQW010000002       155375 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFBQW010000002 DAFBQW010000000
VERSION     DAFBQW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 155375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 155375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..155375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.002"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..2185)
                     /locus_tag="LK889_000161"
     CDS             complement(<1..2185)
                     /locus_tag="LK889_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK5094431.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2403..3086
                     /locus_tag="LK889_000162"
     CDS             2403..3086
                     /locus_tag="LK889_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBK5094432.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3167..3886)
                     /locus_tag="LK889_000163"
     CDS             complement(3167..3886)
                     /locus_tag="LK889_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5094433.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4198..4404)
                     /locus_tag="LK889_000164"
     CDS             complement(4198..4404)
                     /locus_tag="LK889_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094434.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4649..5251
                     /locus_tag="LK889_000165"
     CDS             4649..5251
                     /locus_tag="LK889_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK5094435.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5392..6672
                     /locus_tag="LK889_000166"
     CDS             5392..6672
                     /locus_tag="LK889_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5094436.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6685..7413
                     /locus_tag="LK889_000167"
     CDS             6685..7413
                     /locus_tag="LK889_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5094437.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7482..9005)
                     /locus_tag="LK889_000168"
     CDS             complement(7482..9005)
                     /locus_tag="LK889_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK5094438.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9257..10438
                     /locus_tag="LK889_000169"
     CDS             9257..10438
                     /locus_tag="LK889_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5094439.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10498..12126)
                     /gene="treC"
                     /locus_tag="LK889_000170"
     CDS             complement(10498..12126)
                     /gene="treC"
                     /locus_tag="LK889_000170"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK5094440.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12577..15288)
                     /locus_tag="LK889_000171"
     CDS             complement(12577..15288)
                     /locus_tag="LK889_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094441.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15298..15999)
                     /locus_tag="LK889_000172"
     CDS             complement(15298..15999)
                     /locus_tag="LK889_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094442.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16185..16784
                     /locus_tag="LK889_000173"
     CDS             16185..16784
                     /locus_tag="LK889_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5094443.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16865..17878
                     /locus_tag="LK889_000174"
     CDS             16865..17878
                     /locus_tag="LK889_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK5094444.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            17986..18903
                     /gene="rbsK"
                     /locus_tag="LK889_000175"
     CDS             17986..18903
                     /gene="rbsK"
                     /locus_tag="LK889_000175"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK5094445.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            18931..19326
                     /gene="rbsD"
                     /locus_tag="LK889_000176"
     CDS             18931..19326
                     /gene="rbsD"
                     /locus_tag="LK889_000176"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK5094446.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19326..20699
                     /locus_tag="LK889_000177"
     CDS             19326..20699
                     /locus_tag="LK889_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5094447.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21056..23200
                     /locus_tag="LK889_000178"
     CDS             21056..23200
                     /locus_tag="LK889_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK5094448.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23252..24292)
                     /locus_tag="LK889_000179"
     CDS             complement(23252..24292)
                     /locus_tag="LK889_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK5094449.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24569..26371)
                     /locus_tag="LK889_000180"
     CDS             complement(24569..26371)
                     /locus_tag="LK889_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094450.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26385..27137)
                     /locus_tag="LK889_000181"
     CDS             complement(26385..27137)
                     /locus_tag="LK889_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094451.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27146..27508)
                     /locus_tag="LK889_000182"
     CDS             complement(27146..27508)
                     /locus_tag="LK889_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5094452.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27684..28556)
                     /locus_tag="LK889_000183"
     CDS             complement(27684..28556)
                     /locus_tag="LK889_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK5094453.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28607..29095)
                     /locus_tag="LK889_000184"
     CDS             complement(28607..29095)
                     /locus_tag="LK889_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBK5094454.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29233..30024)
                     /locus_tag="LK889_000185"
     CDS             complement(29233..30024)
                     /locus_tag="LK889_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBK5094455.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30079..30687)
                     /locus_tag="LK889_000186"
     CDS             complement(30079..30687)
                     /locus_tag="LK889_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBK5094456.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30680..30997)
                     /locus_tag="LK889_000187"
     CDS             complement(30680..30997)
                     /locus_tag="LK889_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5094457.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31153..31881)
                     /locus_tag="LK889_000188"
     CDS             complement(31153..31881)
                     /locus_tag="LK889_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK5094458.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32040..32921
                     /locus_tag="LK889_000189"
     CDS             32040..32921
                     /locus_tag="LK889_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5094459.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33158..34201)
                     /locus_tag="LK889_000190"
     CDS             complement(33158..34201)
                     /locus_tag="LK889_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5094460.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34204..34872)
                     /locus_tag="LK889_000191"
     CDS             complement(34204..34872)
                     /locus_tag="LK889_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5094461.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35014..37350
                     /locus_tag="LK889_000192"
     CDS             35014..37350
                     /locus_tag="LK889_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5094462.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37465..37776)
                     /locus_tag="LK889_000193"
     CDS             complement(37465..37776)
                     /locus_tag="LK889_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094463.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            37957..39432
                     /locus_tag="LK889_000194"
     CDS             37957..39432
                     /locus_tag="LK889_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK5094464.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39479..40063
                     /locus_tag="LK889_000195"
     CDS             39479..40063
                     /locus_tag="LK889_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK5094465.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40065..40484
                     /locus_tag="LK889_000196"
     CDS             40065..40484
                     /locus_tag="LK889_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBK5094466.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40765..41049)
                     /locus_tag="LK889_000197"
     CDS             complement(40765..41049)
                     /locus_tag="LK889_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBK5094467.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41401..42111)
                     /locus_tag="LK889_000198"
     CDS             complement(41401..42111)
                     /locus_tag="LK889_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBK5094468.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42108..43580)
                     /locus_tag="LK889_000199"
     CDS             complement(42108..43580)
                     /locus_tag="LK889_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5094469.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43718..44191
                     /locus_tag="LK889_000200"
     CDS             43718..44191
                     /locus_tag="LK889_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5094470.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44344..44895)
                     /locus_tag="LK889_000201"
     CDS             complement(44344..44895)
                     /locus_tag="LK889_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5094471.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45000..45530
                     /locus_tag="LK889_000202"
     CDS             45000..45530
                     /locus_tag="LK889_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBK5094472.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46013..46456)
                     /locus_tag="LK889_000203"
     CDS             complement(46013..46456)
                     /locus_tag="LK889_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094473.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46472..47632)
                     /locus_tag="LK889_000204"
     CDS             complement(46472..47632)
                     /locus_tag="LK889_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5094474.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48139..49839
                     /locus_tag="LK889_000205"
     CDS             48139..49839
                     /locus_tag="LK889_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094475.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(49941..50774)
                     /locus_tag="LK889_000206"
     CDS             complement(49941..50774)
                     /locus_tag="LK889_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK5094476.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51078..53243)
                     /locus_tag="LK889_000207"
     CDS             complement(51078..53243)
                     /locus_tag="LK889_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5094477.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFATKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53503..55797
                     /locus_tag="LK889_000208"
     CDS             53503..55797
                     /locus_tag="LK889_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK5094478.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55790..56455
                     /gene="pgmB"
                     /locus_tag="LK889_000209"
     CDS             55790..56455
                     /gene="pgmB"
                     /locus_tag="LK889_000209"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK5094479.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56448..57455
                     /locus_tag="LK889_000210"
     CDS             56448..57455
                     /locus_tag="LK889_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK5094480.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57477..58496
                     /locus_tag="LK889_000211"
     CDS             57477..58496
                     /locus_tag="LK889_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5094481.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58777..60145)
                     /gene="nhaC"
                     /locus_tag="LK889_000212"
                     /pseudo
     CDS             complement(58777..60145)
                     /gene="nhaC"
                     /locus_tag="LK889_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
     gene            complement(60254..61675)
                     /locus_tag="LK889_000213"
     CDS             complement(60254..61675)
                     /locus_tag="LK889_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5094482.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(61918..63795)
                     /gene="tdc"
                     /locus_tag="LK889_000214"
     CDS             complement(61918..63795)
                     /gene="tdc"
                     /locus_tag="LK889_000214"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK5094483.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64086..65342)
                     /locus_tag="LK889_000215"
     CDS             complement(64086..65342)
                     /locus_tag="LK889_000215"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK5094484.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65454..65659)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            65922..67223
                     /locus_tag="LK889_000216"
     CDS             65922..67223
                     /locus_tag="LK889_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK5094485.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67292..67891)
                     /locus_tag="LK889_000217"
     CDS             complement(67292..67891)
                     /locus_tag="LK889_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5094486.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68145..69026
                     /locus_tag="LK889_000218"
     CDS             68145..69026
                     /locus_tag="LK889_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094487.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69039..70649
                     /locus_tag="LK889_000219"
     CDS             69039..70649
                     /locus_tag="LK889_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBK5094488.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70726..71385)
                     /locus_tag="LK889_000220"
     CDS             complement(70726..71385)
                     /locus_tag="LK889_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5094489.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71668..72333)
                     /locus_tag="LK889_000221"
     CDS             complement(71668..72333)
                     /locus_tag="LK889_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBK5094490.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72480..73085)
                     /gene="dhaL"
                     /locus_tag="LK889_000222"
     CDS             complement(72480..73085)
                     /gene="dhaL"
                     /locus_tag="LK889_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBK5094491.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73113..74123)
                     /gene="dhaK"
                     /locus_tag="LK889_000223"
     CDS             complement(73113..74123)
                     /gene="dhaK"
                     /locus_tag="LK889_000223"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK5094492.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(74098..74544)
                     /gene="dhaM"
                     /locus_tag="LK889_000224"
     CDS             complement(74098..74544)
                     /gene="dhaM"
                     /locus_tag="LK889_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBK5094493.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74647..74967
                     /locus_tag="LK889_000225"
     CDS             74647..74967
                     /locus_tag="LK889_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBK5094494.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75079..75948)
                     /locus_tag="LK889_000226"
     CDS             complement(75079..75948)
                     /locus_tag="LK889_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5094495.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(76044..77348)
                     /locus_tag="LK889_000227"
     CDS             complement(76044..77348)
                     /locus_tag="LK889_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5094496.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77345..77854)
                     /locus_tag="LK889_000228"
     CDS             complement(77345..77854)
                     /locus_tag="LK889_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094497.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78029..80164)
                     /locus_tag="LK889_000229"
     CDS             complement(78029..80164)
                     /locus_tag="LK889_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5094498.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80247..81902)
                     /locus_tag="LK889_000230"
     CDS             complement(80247..81902)
                     /locus_tag="LK889_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5094499.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(81904..82218)
                     /locus_tag="LK889_000231"
     CDS             complement(81904..82218)
                     /locus_tag="LK889_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK5094500.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82388..82648
                     /locus_tag="LK889_000232"
     CDS             82388..82648
                     /locus_tag="LK889_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBK5094501.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82662..82976
                     /locus_tag="LK889_000233"
     CDS             82662..82976
                     /locus_tag="LK889_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBK5094502.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(83706..84260)
                     /locus_tag="LK889_000234"
     CDS             complement(83706..84260)
                     /locus_tag="LK889_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK5094503.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84386..86315)
                     /locus_tag="LK889_000235"
                     /pseudo
     CDS             complement(84386..86315)
                     /locus_tag="LK889_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(86621..87337)
                     /locus_tag="LK889_000236"
     CDS             complement(86621..87337)
                     /locus_tag="LK889_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK5094504.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87338..89161)
                     /gene="glpO"
                     /locus_tag="LK889_000237"
     CDS             complement(87338..89161)
                     /gene="glpO"
                     /locus_tag="LK889_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBK5094505.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(89175..90671)
                     /gene="glpK"
                     /locus_tag="LK889_000238"
     CDS             complement(89175..90671)
                     /gene="glpK"
                     /locus_tag="LK889_000238"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK5094506.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(90807..92267)
                     /locus_tag="LK889_000239"
     CDS             complement(90807..92267)
                     /locus_tag="LK889_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094507.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92257..93552)
                     /locus_tag="LK889_000240"
     CDS             complement(92257..93552)
                     /locus_tag="LK889_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5094508.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(93884..94561)
                     /locus_tag="LK889_000241"
     CDS             complement(93884..94561)
                     /locus_tag="LK889_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBK5094509.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94591..95682)
                     /locus_tag="LK889_000242"
     CDS             complement(94591..95682)
                     /locus_tag="LK889_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBK5094510.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(95783..96217)
                     /locus_tag="LK889_000243"
     CDS             complement(95783..96217)
                     /locus_tag="LK889_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5094511.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(96801..98258)
                     /gene="ascB"
                     /locus_tag="LK889_000244"
     CDS             complement(96801..98258)
                     /gene="ascB"
                     /locus_tag="LK889_000244"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5094512.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98255..100150)
                     /locus_tag="LK889_000245"
     CDS             complement(98255..100150)
                     /locus_tag="LK889_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5094513.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100208..101983)
                     /locus_tag="LK889_000246"
     CDS             complement(100208..101983)
                     /locus_tag="LK889_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK5094514.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(102033..103529)
                     /locus_tag="LK889_000247"
     CDS             complement(102033..103529)
                     /locus_tag="LK889_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5094515.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(103723..105345)
                     /locus_tag="LK889_000248"
     CDS             complement(103723..105345)
                     /locus_tag="LK889_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK5094516.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105673..106986)
                     /locus_tag="LK889_000249"
     CDS             complement(105673..106986)
                     /locus_tag="LK889_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK5094517.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107231..107455)
                     /locus_tag="LK889_000250"
     CDS             complement(107231..107455)
                     /locus_tag="LK889_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094518.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107475..107897)
                     /locus_tag="LK889_000251"
     CDS             complement(107475..107897)
                     /locus_tag="LK889_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBK5094519.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(108040..108750)
                     /locus_tag="LK889_000252"
     CDS             complement(108040..108750)
                     /locus_tag="LK889_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094520.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(108768..109265)
                     /locus_tag="LK889_000253"
     CDS             complement(108768..109265)
                     /locus_tag="LK889_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094521.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109302..109754)
                     /locus_tag="LK889_000254"
     CDS             complement(109302..109754)
                     /locus_tag="LK889_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5094522.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(109889..110494)
                     /locus_tag="LK889_000255"
     CDS             complement(109889..110494)
                     /locus_tag="LK889_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5094523.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(110944..111564)
                     /locus_tag="LK889_000256"
     CDS             complement(110944..111564)
                     /locus_tag="LK889_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK5094524.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111566..112435)
                     /locus_tag="LK889_000257"
     CDS             complement(111566..112435)
                     /locus_tag="LK889_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBK5094525.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112458..112865)
                     /locus_tag="LK889_000258"
     CDS             complement(112458..112865)
                     /locus_tag="LK889_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK5094526.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(112884..114173)
                     /locus_tag="LK889_000259"
     CDS             complement(112884..114173)
                     /locus_tag="LK889_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5094527.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(114200..114502)
                     /locus_tag="LK889_000260"
     CDS             complement(114200..114502)
                     /locus_tag="LK889_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5094528.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(114527..116620)
                     /locus_tag="LK889_000261"
     CDS             complement(114527..116620)
                     /locus_tag="LK889_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5094529.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(117109..117873)
                     /locus_tag="LK889_000262"
     CDS             complement(117109..117873)
                     /locus_tag="LK889_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBK5094530.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(117984..118853)
                     /gene="mdcB"
                     /locus_tag="LK889_000263"
     CDS             complement(117984..118853)
                     /gene="mdcB"
                     /locus_tag="LK889_000263"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBK5094531.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(118878..119954)
                     /locus_tag="LK889_000264"
     CDS             complement(118878..119954)
                     /locus_tag="LK889_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBK5094532.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(119978..120571)
                     /locus_tag="LK889_000265"
     CDS             complement(119978..120571)
                     /locus_tag="LK889_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBK5094533.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(120575..122251)
                     /gene="mdcD"
                     /locus_tag="LK889_000266"
     CDS             complement(120575..122251)
                     /gene="mdcD"
                     /locus_tag="LK889_000266"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBK5094534.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(122241..122540)
                     /locus_tag="LK889_000267"
     CDS             complement(122241..122540)
                     /locus_tag="LK889_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBK5094535.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(122642..124288)
                     /gene="mdcA"
                     /locus_tag="LK889_000268"
     CDS             complement(122642..124288)
                     /gene="mdcA"
                     /locus_tag="LK889_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBK5094536.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(124295..125206)
                     /locus_tag="LK889_000269"
     CDS             complement(124295..125206)
                     /locus_tag="LK889_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK5094537.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            125326..126183
                     /locus_tag="LK889_000270"
     CDS             125326..126183
                     /locus_tag="LK889_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5094538.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(126370..126732)
                     /locus_tag="LK889_000271"
     CDS             complement(126370..126732)
                     /locus_tag="LK889_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK5094539.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            127078..128517
                     /locus_tag="LK889_000272"
     CDS             127078..128517
                     /locus_tag="LK889_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094540.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(128524..131076)
                     /locus_tag="LK889_000273"
     CDS             complement(128524..131076)
                     /locus_tag="LK889_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBK5094541.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(131190..132521)
                     /locus_tag="LK889_000274"
     CDS             complement(131190..132521)
                     /locus_tag="LK889_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5094542.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(132487..133965)
                     /locus_tag="LK889_000275"
     CDS             complement(132487..133965)
                     /locus_tag="LK889_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5094543.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(134270..136105)
                     /gene="tdc"
                     /locus_tag="LK889_000276"
     CDS             complement(134270..136105)
                     /gene="tdc"
                     /locus_tag="LK889_000276"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK5094544.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            136575..138002
                     /locus_tag="LK889_000277"
     CDS             136575..138002
                     /locus_tag="LK889_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094545.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(138094..138459)
                     /locus_tag="LK889_000278"
     CDS             complement(138094..138459)
                     /locus_tag="LK889_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK5094546.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(138456..138821)
                     /locus_tag="LK889_000279"
     CDS             complement(138456..138821)
                     /locus_tag="LK889_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK5094547.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(139013..139261)
                     /locus_tag="LK889_000280"
     CDS             complement(139013..139261)
                     /locus_tag="LK889_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094548.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            139260..139388
                     /locus_tag="LK889_000281"
     CDS             139260..139388
                     /locus_tag="LK889_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBK5094549.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            139445..141139
                     /locus_tag="LK889_000282"
                     /pseudo
     CDS             139445..141139
                     /locus_tag="LK889_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
     gene            141143..141262
                     /locus_tag="LK889_000283"
                     /pseudo
     CDS             141143..141262
                     /locus_tag="LK889_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            141239..141535
                     /locus_tag="LK889_000284"
     CDS             141239..141535
                     /locus_tag="LK889_000284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094550.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            141913..143046
                     /locus_tag="LK889_000285"
     CDS             141913..143046
                     /locus_tag="LK889_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBK5094551.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            143077..143397
                     /locus_tag="LK889_000286"
     CDS             143077..143397
                     /locus_tag="LK889_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBK5094552.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(143426..143890)
                     /locus_tag="LK889_000287"
     CDS             complement(143426..143890)
                     /locus_tag="LK889_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094553.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(144022..145276)
                     /locus_tag="LK889_000288"
                     /pseudo
     CDS             complement(144022..145276)
                     /locus_tag="LK889_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(145305..145622)
                     /locus_tag="LK889_000289"
     CDS             complement(145305..145622)
                     /locus_tag="LK889_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5094554.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(145648..145968)
                     /locus_tag="LK889_000290"
     CDS             complement(145648..145968)
                     /locus_tag="LK889_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5094555.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(146432..147847)
                     /locus_tag="LK889_000291"
     CDS             complement(146432..147847)
                     /locus_tag="LK889_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5094556.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(147864..149180)
                     /locus_tag="LK889_000292"
     CDS             complement(147864..149180)
                     /locus_tag="LK889_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5094557.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(149330..150028)
                     /locus_tag="LK889_000293"
     CDS             complement(149330..150028)
                     /locus_tag="LK889_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK5094558.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(150317..151273)
                     /locus_tag="LK889_000294"
     CDS             complement(150317..151273)
                     /locus_tag="LK889_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5094559.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(151386..152219)
                     /gene="msrA"
                     /locus_tag="LK889_000295"
     CDS             complement(151386..152219)
                     /gene="msrA"
                     /locus_tag="LK889_000295"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5094560.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(152394..154022)
                     /locus_tag="LK889_000296"
     CDS             complement(152394..154022)
                     /locus_tag="LK889_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094561.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(154009..155343)
                     /locus_tag="LK889_000297"
     CDS             complement(154009..155343)
                     /locus_tag="LK889_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094562.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        45870 a        32098 c        26970 g        50437 t
ORIGIN      
        1 actttccttt tttcacacga ctcccggttt ctttgataaa tctcgatact ttcaattttc
       61 ttgaattttt catttgaggt acacatagat acagacgatt tcttgctcta gtcatcgcaa
      121 cataaaatag tcttcgttct tcttctatcg tcgttactgg ttctgctttt atttcattaa
      181 agtcgattac tttcttttct gtcgctccgt tataaggcaa acttccttcg ttacaaccaa
      241 tcaaaaagac atttcggtaa ctaagacctt tacttttgtg aatgctctgg atacatagtg
      301 catcaagttt tccttgtctg taagcaatca agcgggtttt taattcttta gctgtcgctt
      361 gggtttcttg cgataaggct tggagtcttc tgaaagtagc atgctgcttt acttcttctc
      421 gtatccattc ttgatcactc tggtcgattt ccttagctat acgtggcaga tgaagatagc
      481 tttgaacaag cgaatagatt tcttgatgct ggtctgtttt acgcaacgct tcctcaaact
      541 ctcggatttc tgcagatttc gattttaaat tggggcttat cgacaaaata taatctgcaa
      601 cggatatatt ttgatattta ggagaaaagt attgatcgca ttcacttttt ttcaggtagc
      661 agtctgccat cagctgccgg agcaattgct ggtaggcctg atttttcttt tgcagattca
      721 gtgctgtttg ccaatgaaca attttttgat agaaatgttg gaaagacttg ataccaaaga
      781 taccttcata tggactatct gcttccagta caaaaggaat attcgttttc agcagagatt
      841 ggacaagctg ctgcgtttgt gaagctgatc ggtataatat gattgattcc ttatatttcc
      901 catttttttg aataaatgtc tgaatttgtt tgattagcca ctgactttct gctttagtat
      961 cagaaaacaa ataggcttcc ataacacctg cttctttttg tgctggaatc atcgcctttg
     1021 gtatccgatc atttttatta tagctgatca atctatttgc tccttggaca atagctgccc
     1081 gagaacgata attagttaat aagaaaagag ttttggtagg atattctttt tcaaagttct
     1141 tgatgtatgc tggctctgat cctctgaatc catagatact ctgatcatca tctccaacaa
     1201 caatcaactt gatcctgttt tcctttgtca tccgtttcac gatttcccat tgaagtggat
     1261 tagtatcttg aaattcatca atgaatagat aatcatattt ttggccgata taatgctgga
     1321 tctcctcatt ctccaaagcg cttttcagat tttggagtac atcattgaac tctagcaatt
     1381 gctggcttct tttttgttct tggagttccg tgagcaatcc atacagtcgt tctgatatcg
     1441 gctgtcgtgc attcttatca atggaatcat atttttcttt aactaactga accatttctt
     1501 ctactgaaag gttcatattc tgccaatagt ctaaccgaga agcaatcgtt ttttctccat
     1561 attgatcaaa tatataaaga ttatatttac gtatcagctg ttgcaaaaca gcattcactt
     1621 tttgtgcagt atcctttgaa aagtctcctt gtattccttg cattgctact tcaggaaatt
     1681 ttcgacaaat ttcattgtat atcctaaaaa agatactatg gaatgttccg atatggacag
     1741 cggtttttct ctggctgaaa ttatctactc gtttttttag ttcatttgca gcgttccttg
     1801 taaaagtgat cattagtatg cgttcttcgt ctacttgttt ttgcgtcatc agataaacag
     1861 cttttgcaca gattgtttgc gttttgccgc tcccagctcc tgctatgatt cgaagatgtt
     1921 tcttcatatc atatgtgata gctgcttttt gttcttgatt gaagcgatta taggaacgtt
     1981 tctccatcat tttaaagaaa gagctgtttc cttcactttc ttttacttgt acttcttcct
     2041 caataagtcc gggtgcttcg ccataagccg cttcataggt agcaatgatt tcttcttgta
     2101 gccgaaagac ttgttgaagc tttttatact tgttctccca gcgtttcaat tctttttgta
     2161 atcggagaat ttctttttca ttcaactgat tgatttcctt cccatctttc ttctttgaat
     2221 ttgtttattt caagttggtt atttaaagtt tcgttgtcta ttttttcttt atactaacag
     2281 acctttctaa aaatgaaaat gggaagcgat atgattttag ttttctgaaa atttttcctt
     2341 tttttcaatc gtttatgcta agataaggaa gacaaaaata ttcataaaag gtgatgaaaa
     2401 aaatgcagac ttcctttcac gaggggcgta caataggact tttgctgaca tttatcggag
     2461 gagccatgga cagttatact tatatccaat atggtgcttt tgcctctgct caaactggca
     2521 atatcattct atcgatcatc caagcatttg acggagaatg gctaagtgtt ggtaaaaaag
     2581 tattgtctac ccttttcttt ttcttaggga ttcttctaac aaagtatcta attgattatt
     2641 ttagaaaaaa agaaaaacat ttctggcgct tgtttgttct atattatgaa gcagtgattt
     2701 ttttctttgt tagtttgaca cccgtccatg agcatcccgc attagtaacg attttgattg
     2761 cttttactgc atcgatccag tgggtagctt ttgataaaat caacgggcgt gcctacacta
     2821 atttatttac aactggaaat ttaaaagggg tagcaacgaa cttatatgat tattgtattt
     2881 ctaaagaaaa agcagatttt gatcgatttt tccactatct aagtgtcgtt cttgccttta
     2941 tcgcaggagc tattgtcctt gttttctgtc atcatctgtt tggcgcccat tcgattttga
     3001 tcgtttccat tttatttttg atcatggccc tcactcagac atttcattta tggcaatttt
     3061 atcatacgaa tcggttcact atttgatctc aatgaatcaa aaaaactaag caagatgctg
     3121 ctcacagaga aatatctgcg agtgagcaat ttgcttagtt tttatattat ttagttgtaa
     3181 agattccctt tgctttcggg ttatttgtag tgtagcggat cgactgggca tctgcctctt
     3241 ctgtcatttt ctttgcccgc atcttgattt cttgtacgat ttgttctcca catgccggat
     3301 cggctacgaa atcatatttg tccccatcga tcactatttt gggacatata tcaaactgtt
     3361 cataccactc ttcgtatcgt cgattgagtt ctttatagta ggaatagagt tcaggatctt
     3421 gttctagctg ttcatattcc cggcctcttt ttttgattct cgctaacata gtttcaaagg
     3481 atacacgaat ataaaccagt agatctggtt tcttttgtgg cgaaatttct tctaactcct
     3541 tcagcatcgt ttctaaaaga ccttcatact gctttacttc tacttcagaa acacgcccta
     3601 aatctgcatt caaatgaaac agcatactat cttcgtagat ggagcggtct agtacgttgt
     3661 catcatggct gaaagcatct ttgattgcca aaaagcgttt gttcaaaaag aaaatctgta
     3721 ataaaaaagt atacttttct ggattagaat aaaatagcgg caatacttca ttatcttcca
     3781 cgttctcata aaaaggtttt gtttctaaat cttgggcaag catttcagtt agtgtgcttt
     3841 tccctgcacc gatcgttcct gcaacaatga tcacaattat cggcatcctt cctgtaataa
     3901 aatctgttct tcattttact acttcattta tagaaataaa agccttgact ttatctgctc
     3961 cttaaaagct acttctctaa aagtagccaa aagtaactcc tattcatgaa aataaaggct
     4021 gtgacaaaag tcttgtcgca gccctttttt caaataaaca gtattctcct gccaacacct
     4081 cggtcacaag gcaatattca taaaacatga aaagctgttt tttaacatta ttccttgtaa
     4141 ctcagagcta aacgggagac tcacaacctc tttatgctct caacatttta tctgttttta
     4201 ttcttctatt gtacttgatt tgcgtacaac taactgccag tgcagattat gagcggtatc
     4261 attgtcttct actgcgcatt tcatgacacg tatcatcaca ctattttcat agattttttc
     4321 tatttttcct tttattggtg attcaaataa atcagattta aatgcatatg tttcgccgac
     4381 ttcaaacaat tgttcatttt ccataagaat ccctccatca ccttttataa ggtcagtaat
     4441 tttttaacat aaatcactcg aaaaagcaca aacttgtcat aaaaagtact tttcgtatca
     4501 aaactgtcac tttttttggt aaggttcttg gaaaacgaag atttttttag gagatcatga
     4561 cggttttgat gtcaaaacca ctttttatga aacaaaagac gttttttatt ttttttagca
     4621 taaaatttgt ggtggaggta cgtatgagat gaaagataaa gttgtgaaca aattattaga
     4681 agccttattc agcggagaca aatcggaaga agatatggtg tatgtccaag taaaatttgt
     4741 cttagaagtt ttattgaata atcttggaaa actagcggtc gttctagtct tttctctggt
     4801 gacaggctca tgggcggaaa caggaatcac ctttctttct tatatctgca tccgcagata
     4861 tgcttatgga ttgcattccg actctgaatt cgtttgtctg ctgtggacac tactttattt
     4921 atggggcgtt cccttggtga tgaaacatct gcaattgact atctccttcc caatgatggt
     4981 ctttttgtta atcagctgct ttttgttact gcttcgctat ggatcaagag gtacagcaat
     5041 caatcccatt gaacccgaga aacgtccccc attattaaag aaggcgatca gcatgtttct
     5101 aattttttct ctcattacgt tatttttctc agcttcttac ttttctacct atttgctact
     5161 tgggattgtt ttagaaatag ctacgttgct tcctattaca aactacttga tgaattttgg
     5221 aggaatcttt catgaaaaaa acaatcatta aacaattaac taacttatct gacttttttg
     5281 tatctggctg ctgctttggt atgagttctg aattcaaaat ccctgaaaaa ttaaaaaaat
     5341 aatacgccaa ttgtgctata atttggtaaa aatcaatggg agctgtactt tatgttttta
     5401 tggtccatag cgttgcaata tttgatttac acaatttgta tcttattgat tgaccatcac
     5461 atccgataca aatatctgtt tgcgggagtc attctgattt cttgtatttt gattggatgg
     5521 gtctattcga ttttagggat ttttactgct ctaattgcta taggaatgct gatgcttact
     5581 tttttcttct tttcgacttc gcgtcaacaa ttggtttacg cgcttccttt tgggttattg
     5641 acgtcgcttt taggagatca cttaacaagc atcggcgatt tttttgtttt gaaatcttcg
     5701 gttattgaac caggagattc attgcagtat tttcacattg tcttatccgc ttttttatct
     5761 gctttattag ctttttgttt aaaaaaatcg ttagagactt ggtttcacac tgtggaccgc
     5821 cgtatgatcg gtacgatcgg gacattgata ttatttacat actatatcat tattttttat
     5881 acccgttttt ctggtgaaac acctgatgtc ttagctttga atacgagttt tttcattcta
     5941 tatctgtgtg ttggcctgat catcttggtt tattattgga aaatctctaa taagaaatta
     6001 gctgatcagt tattagagca acaagtcagg atgcaacagg attatatcaa agatctagaa
     6061 aaaaactatc aagacttgag agaattcaaa catgattacc aaaatctgct tttttctctt
     6121 cacagctata tttcagaagg cgacttagaa ggattacggt cttattatga tcaaacgata
     6181 ttacctacac gccagatgat gaaccttttt ccggccaatc taagtatgtt ggataatatg
     6241 aaagttcctg agatccgtag tttactgtca ttaaaactca tgatggcaca ggaaaaaggg
     6301 ttgatcgtcc agcttcattt tcctgagcca atcgaactgg atacgaaaca tacggtaaac
     6361 cttgtacgta tgcttggtat tatcttggac aatgcgattg aaggagctag tgctgcaaag
     6421 aagaaaaaaa tcgaattgac catactcaag aagaatcatt ccgtgatgat tcgtgtgatc
     6481 aatacaacaa caaatacatt accgttaaac cagttgaaac aaaaagggtt ctcaacaaaa
     6541 acaaatccaa aaaacgaagg ccttggcttg tttatcttgg atgaattgac aaagaccaat
     6601 ccttatcttt tccttgaaac atcaattgaa aaccagcgtt tttctcaaac gatttatata
     6661 caaataaaat aaaggagtaa tgatatgatt cccatttata tttgtgaaga cgatccaaaa
     6721 caattagaag ttttatcaac ggtgatcaaa aatagaatca tgattgaaaa cttagacagt
     6781 tatgtccata tttctacctc tgatccggaa gaacttctca atgcagccaa gatcagaact
     6841 tcttcttttg gtatttactt tttagatatc gaactagaag acagtgaaat ggatggtgta
     6901 gcgatcggta aattgatccg tgaattagat cctttaggaa aaataatcta tgtgacttcc
     6961 catacggaaa tgtcgatgaa aatcttaaaa agcaacatcg aaccgactga ttatatcgtc
     7021 aaagaagatc tttatgattt aaaagaaaat gtcgagcata tcctgagcaa aattttcgaa
     7081 gattttcaaa tgtcccaaat ccaaaaagat atcttcaaaa tagagtttaa tgatgagatc
     7141 aaatttttac cgatcaaaga catccaatat ttttctactt cgctaggagc gccacataaa
     7201 ctggaagtcc atttgactca tgcccaatta caattctatg agaagatcaa agaaatcgag
     7261 aaaatgcatc ggaattttat ccgttgtcat aaatcctatg ttatcaatac acaaaatgtg
     7321 cggtcgatta ataaaaaaac cagagaagtc acgttggcaa acggcgagac catcccagcc
     7381 tctatccgtg gattaaagaa attgatttca taaaaagaac cgaatagttt ggaccgacta
     7441 aatgaccagc cgatccaaac ccttcggttc ttttttgcct atcagtgcaa aagcatttcc
     7501 tgtgcaattt cacgtccatc cattgtatct ggataaaatg ttggccaatt ctccatttct
     7561 ttcagcaata cttcttgact ttcatttcct gcatataaat gaaaatgttc ggagtcagcc
     7621 ggtgcaatcg tgtgatcact gaattgaata tatctaaaag cgccactttc cttatcttct
     7681 gcttcgaata gataccttac gccacggttt cctgcctcgt aggtcaagat ttctttccct
     7741 acataacgat aatttgaacg tttcgttgtt ccattcttaa acgtaaatgc catctctttc
     7801 tcactgatct tgattgaagc aatatctgtt tgatagccct tcgtataata ttctttatat
     7861 tcttgtgctg tcttatcctg attcaatttt tctttatatt caaaaacagg atcaaaagtc
     7921 ccatcttcta catacgtatc tactgtttgc caagttcctg cccaatctga aagagagcga
     7981 tcttgtacat cctcgtcttc aaaataaccg ttgtagacgc tcttttcttt tttgggggtc
     8041 aaagtatctt ctagcgggtt ttctgtctct gtcgtttttt tcaacgcttc gaggtttgct
     8101 tccatgatag aaagatagtt ttctccattt tctatttgtt cattcgtcaa tccttccaaa
     8161 ggattcaata cttctaaaga tacacctgct tcgtttgcta acgttctggc aatactatct
     8221 ttggcgtttt cttcaaaata aatatagttg attccatgtt ctttaacaaa gtgtttcagt
     8281 tcagcaattc tcgccgcaga cggttcttca ctagaagaaa gaccagcaat cgatacttga
     8341 ttcaatccat attctaacgc tagatagttg aatgcagcat gttgcgtgac aaaagatctt
     8401 tgttttgctt gcttaaaggt ttcttgataa agctgatgga gtgcttgaag tttctcggta
     8461 taggctgctg cattttttgt ccatacttcc tgtttttctg ggtaagcttc aatcagctga
     8521 tcacgtatgt ttgctacctg cttgattgct aaacttggag ctagccatac atgtgggtct
     8581 agctcatgtg tatggccttc ttccccgtgt tcatggtcat gatcatgtcc ttcttcactt
     8641 cccggaagta aaagcatacc ttctgtcgct tttatcgtat ggacattgcc ttcttgtaat
     8701 gttttttgga ttgcggggac ccatgtttcc atgttttcat cattataaac aaagatatct
     8761 gtatcttgga tctttgccat gtctttagca gaaggttcat aatcgtggga atcagtccct
     8821 gctggaatca ataactctac ttctccttca tccccgacga ttgcttttgt aaaatcatac
     8881 atgggataaa aggtcgtcat cacgctcaat ttttctttac tgtcctcggc tttcccactt
     8941 gccccgcaag cagttaacat acctgctaaa accagcatgg ccaatatacc tatcaatcgt
     9001 ttcatttatg acactcctct tctcatcttc tttcatttga atgttccgat tcgcttttcg
     9061 caaaccgtaa tagttacgtt ttagcaacag gatttaattt atcatatggt taaaagaact
     9121 gtcaagcgtt ttcttatttt aattgatgag aaagtttata tgaatgattg acgatatttt
     9181 tgcaatcaac ctctatcatt ttccttttat acccgctttt gtctataatg aagttataga
     9241 taaaggagga aaaagagtga aaaaagaaac agatacaacg aatcgtttga tccgcttttt
     9301 aggaggaaaa acctcttact atgttttagg aatagttatc ttacttgcgc tgatcattta
     9361 ttttttccat cagatctcgt ttatattcaa gcctgtcgga atcattatcg ctactatctt
     9421 gcccccatta ttgtttgcac tgatcttgta ttatcttttg gaaccagtca tcaaacgttt
     9481 gagcaagcac ctttcgagga acttgtcagt cattatcgtt tatgtgatta ttttattttt
     9541 attggggctt ggaagcatat ggcttattcc ttttcttgaa gaacaggcaa agacattgat
     9601 cagtagtttg ccagatttgt ttcaagattt tcaagagagt ctccgacaat ttttggagaa
     9661 aacacctttt gccgattcat ttaatcaatt tttcgcttct attgacgacg tgacgtctga
     9721 tatagctgga tttattggaa attattggga aagtggcgca caacggatca gcaacatttt
     9781 ttcaactgtt acagctattt ttattacttt atttactggt ccaattgtcg cttttttcct
     9841 tttgagagat cctcaaaaat tttatcagtc agttttagcg attgtcccac cagcatttcg
     9901 tacagatttt aaaaatttga caaagattgc taatcaacaa ttgggttcat ttctaaaggg
     9961 gcaaatcatt gcttcattta tccttggagc agtctactgg gtttgtttct tactgatcgg
    10021 attagaattt gctagtgttt tagctatttc tgctgggttg ttatgtatca ttccatatat
    10081 tgggccgttt atcgccttct ttccaggttt gatcattgca ttccaagact ctacttttat
    10141 ggctgtcaaa tttgtcattg tctggtttgc cgtacaactt ctccacggtg acttggtgat
    10201 tcctcgagta atgggtgatc gtttacagat ccatccgatt acgatcttaa tcgttttact
    10261 tgtcatgggt gatctaatgg gaattgtggg cgttattttt ggtattccta tctatacgtt
    10321 agtcaaatta ctcgttactt ttattttccg taaattcaaa caacgctaca ataaatttta
    10381 tggcgataaa ggagagtatg aaaactcttc attttctgaa gaagattatt ttaaataaaa
    10441 cacaaaacgt cgatcttacg agaatagctc gtaggaacga cgttttgtgt ttttgtttta
    10501 atttttcagc aaagcaattg cctggtatgg ttttagctcc agactttgat gaagaacagt
    10561 atcggaataa ttatcgataa gaatattggc ttctgtgaat ccttctggga tggcgatcct
    10621 tgtatcttta tcatagaaat ttgttaatac aagcagctgc tgtccttcaa attggcgaag
    10681 aaagccataa acttccggat gatttgcttc ataagcatga taatctcctt gtgcaacgat
    10741 tggatatgtt tttctcagcc gaatcaattt cttgtaatag gacaaaatag aatcttcaga
    10801 agatagttct ttttctacat taatctcttc tagcttgcct ccaactttta accaaggttt
    10861 ccctgtagta aatcctgctt gttctgtgtc tgaccattgc atgggtgttc gagagttatc
    10921 gcgagatttt gctttgatcc gacgaaacgc ttctttttct gtgaatcctt gttcaaggag
    10981 taacttataa gcattcaaac tttctacgtc aatatagtcg gaaatcgatg aatagtctgg
    11041 gtcgatcatg ccgatttctt ctcccatata aatatagggc gtcccacggt tcaaatggat
    11101 catggttgct agcatctctg ctccttgttt tcgatactga tcgatagcta caaatcgatt
    11161 gagcgcacgt ggctgatcat gattattaaa aaatagagca ttccagccgt taccctcact
    11221 catcttttct ccccatgtat gaaatagtcg ctttaattct gcaaaatcaa atggaggagt
    11281 agaccattta ttcccatttt cataatcgac tttcaaatga tggaaattaa agaccattga
    11341 taattcttct ctactaggtt ctgtatacaa gatacaattt tcgatcgtcg tagagctcat
    11401 ttcaccgact gtgatgatag tggaatccgt cccaaacgtt gcttggttca attcttttag
    11461 atacgtatgg gtgattggtt tgtccgtata ttcaggtttg ccatcatttt ctttattatt
    11521 tttcaattgt tcgtctttac cgataacatt gataacatcg aaacggaatc cttttacccc
    11581 tttttccatc caaaaacgaa caactttgaa tagttcctct cttactgctt ggttccgcca
    11641 gttcaagtca gcttgtgtcc gatcatataa gtgcaaataa tatttccccg tatctccaaa
    11701 tggcgaccaa gcacttccac caaattttga tacccaatcc gttggctctt ctcttaaaat
    11761 gaagaagtcc tgataatgct ggtcccctgc tagtgctttt ttgaaccatt catgttctgt
    11821 cgaaacgtga ttcagcacca tatcgagcat gatttctatc cggtatttct ttgcttcggc
    11881 tattagctgt tcgaattcct ccatcgtacc aaagatagga tcgatggctg tgtaatcaga
    11941 gatatcataa ccgttgtctt tttgagggct tggataaata gggtttagcc aaatcatgtc
    12001 gatgcctaga tcattcagat acgggagttt ttctttgatt ccttttaaat ctccaattcc
    12061 atcaccatta ctgtctttgt aagatttggg atagatttgg taaatgactt tgtctttaaa
    12121 tgtcatggat atttacctcc attcaactga tttattacct accacaactt atatatacaa
    12181 gttatcattt attattataa ctgaaccgaa agcgtttgta aaatttatct ttatacattc
    12241 taagaggtct gttgcccaat aaaaaacaag agatcagaca tgtatttgtc tatcctcttg
    12301 tttaaaatag caaaatattc ttgtttcttc tataaatggt gttcaaaagc ttttatctac
    12361 ggcaatcagt cttctcaaat gtaaaacatg aggagctaat tttatttgag tattcttttt
    12421 gctcgatgca ggacggcttt tttacaacct ctgattcatg gtgttcaatc agctgtcctt
    12481 cggtattagc tcaaaaaacg gcaaaatatg aaaaactatt ttgccgtttg tttctctaat
    12541 actcaggtca aaccgctgat ttcacaacct ctgtttttat tcattcgatt tcaacgcatc
    12601 aatcatatcg atatgcttta atttgaaatg ggtaacaatc atcacaataa ccgtaaatac
    12661 gatagttaat cctgctgata agacataagc ccaccatgtg atcactaatg gaaagatgac
    12721 attctccata gacgcttgtt gcaagatgaa gtcagtcaat atatatccta caccaaatcc
    12781 tccgatgatt cctaaaagag taaagatgat attttctcga acaatgtaca tcgtgacttc
    12841 tttatcaaaa aatccaagga cttttattgt agataattcg cgtacacgct cggaaatatt
    12901 gatattagtt agattataca agacgataaa agccagcagt cctgataata cgacaaagat
    12961 taagacaacc gagtctaagt tcgccattga ttcttcttgc gtttcgattt gtttggacat
    13021 gaaagacgta tttgtcacag cgtctgtggc aagcagtttc tcggaaagca tttcttcttc
    13081 ttttgttgtc atcttatctg tacgcagcaa aaatgcatta tccatcatcg attgatcagt
    13141 aactgaagca taatattcag gggacatata aataaagcta cctagatagt ttttcacgat
    13201 ttcttttact tcgactttcc attctttttg atcttcatca taaagcgaaa tatgatctcc
    13261 tgtagataag tcaaatagtt ctgctacttt ttgcgtaata gcgattcctg ttttaggcaa
    13321 ggagcgtggg ttctcttcaa gatcagttaa atggataaaa cgatccaaat cagctgtttt
    13381 acttggtacg atcactgtca gactttgcga agcatgtcct ttttttctca attctacggt
    13441 ttgagtatga atagctagga tctcatttac tttttcttgc ttctgcagca tatccgctgc
    13501 tttttgacga ctatcttccg tggatgaatc caatgtaacg attgcttgat agtcaatgat
    13561 cgttccaaat tgtttggctg aaacagaact tagagaatct ttcaatccca cgcctgcaac
    13621 catcagcccg gcacatccag caattccgat aatcgccata agcattcttg acttatatcg
    13681 gaagagattt cggtaactga ttttttgatt aaagctaagg cgtgaccaca gcggagtgat
    13741 gtattcaaga aaaatgcgtt tccctggctt cggtgctttc ggttgaagca atgcagcagg
    13801 ccgttctctt aaatctttga ataaaacgat cattgcagcg ccaaatgcgg caatgaagaa
    13861 tgcggcagct gcttgtaaaa tcggcggcca gtcataaaaa ataagcgcac tcccaatatc
    13921 ataccgctcg ttagacagct caaaaatgat ccttggcaaa agattcgttc ctaatatcgt
    13981 tcctaaaaca atacctatcc ctgaggctag acttgcatag attgcatatt tttttgcgat
    14041 ttcaactttc gtataaccta acgcctttaa tgtcccgatt tccctgcggt tttcttctac
    14101 catacgtgtc attgtcgtaa aggtgatcaa tgcggcaata aagaaaaaga aaacaggaaa
    14161 aacattggca atcgcagcga tacgatcaga cagatcacca aattcttgaa aaccgacatt
    14221 ttctgaacgt tcattgtata ggtatgatgg tttagttaaa ttttcaattt tttctgcatt
    14281 tcgattcaat tcttcctctg cagaggccag ttcacgttct ttctcaacta attgcctttc
    14341 ttgttcagca agttgagctt cttgttgaga taatgtttga acagcgattg ttcgttgcgc
    14401 ttcaggaagc tgctggatag ttgctgactg ttgcgctaac tgttttttag catcttctaa
    14461 ccgtgttctt gcttgcgttg tttgtgcttt gccatccgct attttttgct gtgccggttc
    14521 taattcttcg ttggccgatt ttttcagctc ttctaagcgt tcttcgggtc gatcttgtaa
    14581 atacttttct gccttttctt gattttgctc cattttatct ttgtaagctt tgctgtacgt
    14641 attcaaattc tttacgtttg taaaatcaag atataagaca gaataaatat ctgttttaaa
    14701 aacggattca ggtaaataga caaaataatc gacgctacca tttccgacgt tcgcgtatcc
    14761 tctctccaaa ttgttgatga acaatggaga acggacaaag cccgtgatgg tatactcttt
    14821 ttctttcaat tcatctgaat caatctgata aatatcaccg attttatacc cttcatcttc
    14881 cgctttttca tccaatacaa gctggtttgg tttttccggg agttttccat cagttaattg
    14941 tagcttgttc tgcttgttat ttttgttata agaaaatatt tgaatcactt cgtttttgcc
    15001 agtattggaa taatagatct gttttcctgt ttctactttt gcatctggga tcttttctgc
    15061 ttgagcaata tctttttgtg tcaagccacc agtagaaacg atttgcaaat cagaaagtcc
    15121 tgctgtacca acatagtgat ccatcgtttt ttgcatgaca ggtccagcag ttttgacacc
    15181 tacaaataaa agtgttccta gcatgacaat aaggatgatt gcaatgaatc gtcctttaga
    15241 ttgccgaatt tcgcgaaaac ctgctttaag atatgttctt gttttcataa aatcccccta
    15301 ccatacaatc tcatcaatgg atgaaggatg atcatttaat tcaacagacc ttactttagc
    15361 atcattaatg tgaatgactc tatcagcgat gggcgctaaa gcagaattat gtgtaatgat
    15421 caaaacggta ttgccgtgtt gtctgctagc gttttgcaat aatttcaata cttgcttgcc
    15481 cgtttcaaaa tccagcgcgc ctgtcggttc atcacataaa agaagtttgg gatttttagc
    15541 tagtgcacga gcaattgaaa ccctctgctg ttcgcctcct gataattggg acggaaagtt
    15601 attcaatcga tgagctaggc ccacttgcct caatacctct acaggatcaa gcgcatcagg
    15661 agaaacttcc gttgccaatt ccacattttc ttttgctgtt agattaggaa cgagattgta
    15721 gaattggaaa acgaacccta catctgttcg tcggtaagca gtaagctgtt tatctgaaaa
    15781 ttgcgcaata tctgtgtcat caataatgat ctgaccttca tcaggtgtat ccatcccacc
    15841 taaaatatta agaatcgttg actttcctgc tccacttgga ccaagtataa tcactaattc
    15901 gcctttttca attgaaaaag aaatatcgtc atttgctgta atggtcgttt ctcccatttt
    15961 gtatcgtttg tattcatttt ttacttcgac atacttcact ctgattcctc cattttcacg
    16021 atatttctcg ctatatctat gcatcgaatg cgttttctca cacttattta ttttaccact
    16081 gaatttctct agactaaaga aaggaatcct ttaatttagt tgacaaataa acgatttatg
    16141 atttacttac ataaaaaaca ttgttatttg aaagaaggaa catcatgaat gatttaaagg
    16201 aaaatatgat ttttgattta tcaaaaactg cttttgtcgc gatcgacatg caaaaagaga
    16261 tcgtaaataa cggtacactc tctccacaca ctgcagcatc tattctgaca aacaatgatt
    16321 tgttagtaag aacattgaaa cgtaccgctg ccttgaaagt tttagtgaac gtagacatct
    16381 ccactttccc ttatttgagt caacaaactg atatgggtgg aatgactgct tctgtacctc
    16441 cagaattcac tgatttactt ttgaaggatt ctttgaaaga tacagataat atgttgacta
    16501 tcacaaaata caatccaagt gctttttttg gtacttctct tgatcttcaa cttcgccggc
    16561 gtggaatcga aactatcatt ttaagcggtg tagcgacaac aaacggtgta tatgctactg
    16621 cattagatgc ctttcaacac gggtaccata tcgtgcttgc tgaagatgct tgcagtgatc
    16681 gtgacaaaga atcccatcaa ttgttcataa aaaagatatt tccgaaaact gcacgagtac
    16741 gctcaacgaa acaaataatc gaagctatcc aacaaagcaa ataagtactg gcacacgtga
    16801 ttttcattta cttctttttg atttcatggg aaaataacca caggatttag ataggagtat
    16861 gaacatggtg aaaaaaaaga tcacaatcaa agaagtcgct aaactttccg gcgtttcaat
    16921 cacaactgtc tccttgatat taaatggaaa tacggagaaa ttcagtccga aaactgttca
    16981 gaaagtttta gctgccaaag aagaattagg ctatcagcct gactattttg ctagacaaat
    17041 gattaccaag gaaacgaaaa caattggtgt actcgttcct gatatcacga atccattctt
    17101 cagtacactg atgcgaggaa ttgaggatat cttgtataag cagaatttcg tcacgatatt
    17161 atgtaatgct gattctgatc atcaaaaaga aatcgaatat ctagccgagc tcacacgtcg
    17221 aggtgtggat ggattcatta tcgcgacttc tgctgtctct acagatgcaa tcaacgaaaa
    17281 tttgaaaaaa caaggccggc ccttcattgt tttggatcag aaaaaatctg aaggatttag
    17341 tgatgccgta cgaacagacg attttcgcgg tggttaccta gcgggcatgc acttgcttag
    17401 tctaggacat caaacgatcg cccttgtcta tcctgaaaat ccaccggaaa atgtccacgc
    17461 tcggattgag gggtttaaat ctgctcttga tgtttaccaa atccctcacg accaactgat
    17521 cctcttacct acacagttct caaaacaagg tggctaccag atcacagcag aacttcttga
    17581 ttctgctgca actggtgtgt ttgctttaaa tgatgaatta gcttttggat tatatcgcgg
    17641 attagaggaa gctggaaaaa gcatccctga agactacagc attattggct atgataatat
    17701 agatatgtgc gagtatatca agccgaaact gacaacgatc gcacaaccaa tcttcgaatt
    17761 gggacaaaca tctgccaagt tattgttaga tcggatccaa ttcccagaaa aagaatggga
    17821 agaaaaaaga ttacctgtac gattagaaaa aagattttca actgcaccct tgaaatagct
    17881 ttcaaggtgt gctatatttt tatcgaacta ttaaaacgtt ttactaaaac gttttaaaaa
    17941 ataattttcc catcaaaata aatgagatag ttggaggttt gtaaaatgaa cacggttaca
    18001 gtaattggaa gtattaatat ggacactacc cttcgtttaa ccaacatgcc caagccaggc
    18061 gaaacgatgc atgcgcatga gatttttcac gctggcggag gtaaaggtgc caaccaagca
    18121 gtggcagcca aacgttcagg cgcacggaca tcttttattg gaggcgttgg ggctgacagc
    18181 gaaggacaac agttattaga tctactgaca aaagaaaaca tcgatacatc tgggatagct
    18241 gaaatccaag gcgccacaac cggacaagca atgattatgg tagacgcagc aggagaaaat
    18301 agtatcttga tccatgcagg ggcaaataat gctttccatg aacaagaagt cctaaaaaac
    18361 aagcaactga ttacaaacag tgactttatt attgctcagt ttgaaagtag cttagatgcg
    18421 acgatcctag ctttttctat tgcaaaagac gcaggaaaaa caacgatcct gaatcctgca
    18481 ccagctcgtg aaacgattcc tactgaacta cttgaaaaga cagatatcat tataccaaat
    18541 gaaacagaaa cagaaatcat tacaggaatc agagtgacag atcataacag tttggtcgca
    18601 gcagccgaaa aactccatga attaggtatt ggaacagtaa tcattacact aggcagtgcc
    18661 ggcgctttct atcatacaga aaaagaacac ggtatcgtcc ctgcttttaa agtggatgcc
    18721 gttgacacaa cagccgcagg tgatacattt attggcgcat taagcagcac acttcagcca
    18781 gaccttagta acttgaaaga agcgatcctt tatggaaatc tagcctcttc tgttgctgtg
    18841 cagtcttatg gtgcgcaacc ttctatccct taccgcgaag ctttagaaca atcagcagtc
    18901 taaaattaac aatgagagga agaaacaaac atgaaaaaag gaaaagtaat caattcagat
    18961 atttcacgag taatcgcaca aatggggcac tttgataaat taagtatcgg ggatgcaggc
    19021 atgcctgtcc cctttggtac tgaaaaaata gatttggctg tctcaaacgg tcttccaagt
    19081 tttttatctg ttttagaaaa cgttttagaa gaacttgagg tacaatcgat tgaattggcg
    19141 gaagaaataa agacaatgaa cccggaaata ttggaaaaaa tcaaattatt gttaccagat
    19201 acaccgatta gctttatccc acatgaagaa atgaaaaaag agttgaataa ctgtcatgca
    19261 tttattcgta caggcgaaat gacaccttac gccaatatta tattagtgag tggagtggtc
    19321 ttctaatgga aacgacaatg aatcctgaac agatggtact gtccgagcag attcatatgc
    19381 tgattgaaca gttgggagga aaagacaata ttgattctgt ccttccatgt gcgactcggc
    19441 ttcgtatcaa attgaaaaaa cagccagtag ccaatgcaga aaaattaaaa cagcaaatcc
    19501 cttccattaa gtatttcatt ttactcgata gatatgcaga aataatcatg gatcagggat
    19561 tggctcctgc ttacaaggaa ttgacgcgtc aaatcgatca ttcagctccg aaagaaaagc
    19621 taaatccgat cgaaaaacta tttggtttga tatcggaaat ctttacgccg atcattccgc
    19681 tgtttgccgg ttctggtata ttaaaaggtc tggttgtttt agcgactacg attggctggc
    19741 ttagtgaaac aagcggaact tatcatattt tgtctgctgc gtcgaatgcc gtcttttatt
    19801 ttctgccaat ctttcttgct ttttctgctg ccaaaacgtt taaagtgaat ggatatattg
    19861 cggctgtcat tgctgcagca ctgattgaac cgaatctgac ctctttactt tcagacagtg
    19921 gcaaagcagc tactgatttt ctggggattc ctgtagtact gatgcagtat acgtctactg
    19981 tcatgccggc gattttaggt attttctttt attcttttgt tgaaaaattc ctcaaaaaat
    20041 atattcgcga aaatatgcaa ttagtatttg tacctttact ttcattagtt atcacggttc
    20101 ctttgacgtt aatggtattc gggcctgcgg gtgtttacct tggcgaaggt ttagctgccg
    20161 caattacttg gatgatggat attaacggac cgatcagtgg cgcaatcatc gctggtggtt
    20221 ggaatatttt ggtaatattt ggtatccaat gggctgtaaa ccctgtcatg atcagtaata
    20281 tctctgctta tgggttcgac cgaatcgttc cgttgacagg agcagccaat tttggaatgg
    20341 ctggtgcagc tttaggtgta tttctgcgaa gtaaacgttc aaaaacccgt tcgatctccg
    20401 gttctgcttt tgcttcgatt ttacttgcgg gagtgaccga accaactgtt tacggaattg
    20461 cgatcccatt gaaaaaacca tttgttgctg cttgcattgg tgcagctgct ggtggagctg
    20521 taatgggatt tgcccaagtg aaagcgatcg cttttgtatt tggtagtctg accacattgc
    20581 ctgcattcat ttccggtact tttttctggt acttagcagg actggctgtt agtctagtcg
    20641 tagcaatgat cactactctc gttagcgggt ttgatgaaga cctaatgtct tatgaatagt
    20701 taatgaaaaa gactgaagta tgagatgttt atcacatatt tcagtctttt tctttttagg
    20761 atcattattt ttataaattt ttgtgatact ttccaaactt tggtataatt tacatatgca
    20821 taaccatttt tatccccact tgtttatccc caaacaaagg tggtctatgc aataaaatga
    20881 acgtcaaaga aaaacgaatg aatgctcgtt gcttatttac aaattgtttt atttagaata
    20941 cacagacaga tttatgaaaa gaaaaagctt ttcgacacga ttatctgttc caaaaaatgt
    21001 ctaatgaatg ctgaataaga cagaaaatca atgaaaggaa aaggactagt ttattatgaa
    21061 aaataaaaaa acgatacgtt tgctagctgc tacttggctg attgctccgt ctgttttgtc
    21121 gaccaatacc gtatttgccg tagataatca aatagctgat caaacagtca caacagatac
    21181 gactacatcc agctcagcta ctagtgaaac aacggattct tttacagaag acccaactgg
    21241 aacaactaac tcaacaacag gatcgaccga gccaactgaa attgatccgg ctgatccatc
    21301 agaaaatgaa aagccagccg agacatattc ccttacaatt gctccttctt tgattggtaa
    21361 gatccaactg actgcttcga cagcggattc ccctgaacag caacctttgg atattacgga
    21421 aagtggaacg gtagaaggtt tattagcgga aacgaaggta acttacacta ttacgccagc
    21481 tgaaggtgaa caaattgctt cagtcgctgt taatggtgcc aaagaagtaa attatgatgc
    21541 aaagaccttt acagtaggta cgaacaattt atctattact gcagaattta aagctggacc
    21601 ggatacttca acagagaaac caactgatcc tagtgaagaa gtccctagta caccagatcc
    21661 ggaaacgcca aacaaaccag atgtgggtaa taacaacaat aataatggtt caaataattc
    21721 aaacaataat aacaataatt cgaacagcgg gaattcttcg aatcaaacaa acccatctgg
    21781 atcgaataac aatggaaata ctcagagaga accaaaccat ccaaacaatg gttcatcgaa
    21841 tggcggaacg aacaatgctt catctcgtcc aaatacagca aatcaaagcg gtatagagaa
    21901 tccatcttct gcttcttctg attttgttgt aaaatcacca attgatgctg tattgcctac
    21961 aaatacaacg aatgtccaac aagcaatcgt tcgagaagca ttcaagcatc ttggaaaacc
    22021 atatgtatgg ggagcaaaag gtccaaatac ttttgactgt tctggtttga cttattacgt
    22081 atatatgaaa gcaaccggtc attatatcgg tggctggaca ggcgaacagc aatatgcagg
    22141 tacgcagatt ccagtaagtc aggcccaacc tggcgacctc gtcttctggg gaccttcttc
    22201 cggtgttact caccatgtag gtatctatat tgggaacgga caatttatcc atgcgcctca
    22261 accaggagat aaagttcgag taacttctat ttcagatttc acgcctgatt ttgctgtccg
    22321 agtaaactta gcaggtttac ctgctgcttc aggttcgctg acaggcggct catctatttt
    22381 agatggatta gatggctcgt tccatttcag tcaaaaccaa acaactgatc agttcttgaa
    22441 aaagattgca gatgatgcac aggaaatcgg gcaaaaagaa ggaatctacg catcagtcat
    22501 gatggctcaa gctatccttg aaagtggatc tggaaacagc ttattatcaa gcgaaccaaa
    22561 tcataacctt tttggtatca aaggatcata taaaggaagc agcgtgacct tcaatacact
    22621 tgaacaagat agttccggac aaagctacca aatccgcgca caattccgta aatatccttc
    22681 ttacaaggaa agtttggaag actatgccga cttgatcaaa aatggactga ctggcaatcc
    22741 tgatttctac aaaccaactt ggaagtcgga aacaaaagac tacaaagaag caaccaaata
    22801 tttggaagga cgttatgcta cagatcgcca atattcacaa aaattgaacg cgatcatcga
    22861 agcgtacgac ctgacaaaat atgatgaacc gaaaaaagaa gcagcaacaa ctgaagaagc
    22921 aaacgctgaa atcctcaata tgtctaagcg attcgatgtg ccaatcaaat ggacaacaga
    22981 tggcgtgcca atcacaggat tagaattttc aacgataaaa cgcgtcaaca acgcttcttc
    23041 catcttccgt gtcgcttcaa tctgggattt gtggaacaat tttacggcaa gacagatacc
    23101 agaaacaact actcgtacag ttaaagctgc aactgttcct gtactaacct tgttaagttt
    23161 agacaaatca ttctggcttg atctgccttt aaagaaatag aataaattaa aaaggggatg
    23221 tgacactgtc acactccctt tttaatttat tctatttctt tatatttatt ttgtattccg
    23281 ttacaaactc gcgtacttgg ttaggttcca gagtgacatc tccaaaactt tcaagtcgct
    23341 ccgctcctgg cagttcttga gtctcaaatg tgataccgcc atgatttact aatttattcc
    23401 cgcgcatttc cggtccatct tctccaaaat ttgctgtaaa aatcacgatt cctgaagcat
    23461 ctgtttttac ttggatcgat actttttcat ctgggcttgt caattccgca gcatttagta
    23521 aaccagattg ttttttcaac aaaaatggat gatcgatgcc gcccactatc attgattggc
    23581 gaaaatcact ggcaaagacc tcacctaatt ttttaggacg ttggaagtca aatgctgtgc
    23641 cacttactga aactacttct cctgtaggcg ttgtatcttt attgagcgta gcaaataatc
    23701 ggctattcaa ccaaagtata tgttcgtcga ttgactgtgc tggatcaccg gttaaattga
    23761 aatagacatg atttgtagga ttgaatactg ttttgcggtc actgatccct tggaccgtca
    23821 ctcgccatgt atcatcgttc gtcagcgtat accgtacttc tacatctaaa gttccaggaa
    23881 atccgttttc catatctggg ctagtctgat agaaaatgac cgatgcttcg ttttcaccat
    23941 tgatgactgt ataatgccac tgcttggctt caaagctagg tgcaccacca tgtaaacaat
    24001 gaccagtgtc cgggtctgtt tttacttcga acgtttcatc atctaatttg aatgtgccat
    24061 tcgtaatccg tccagccata cgtccgattg aagcaccgat ataagggtct ttttctagat
    24121 attcttttgc ttcatcaaaa cctaagatca actctcgttt ttcattatcg attattgttt
    24181 gaaaatgaac aacccgtgcc ccaagatcac tgacagacaa agatgtccca ttttgattcg
    24241 tgatcgtaat caagtgatag ttttcgccaa aattttgttc attgatttcc atgttccttg
    24301 caccctcttt tctttcattt gcactgattc cagcatacat ggaaaccgtt ttcttaacag
    24361 cttattccgt gagtaacgag aaaaaattta ctatattcta ataaaagacc gtgaaaaaaa
    24421 ggttgtgaca aaaatattgt cacaacctct ttttccgaac aaacggtgtt caaaagcttg
    24481 catctgcggt aataagccca accaaacgaa aaatatgagg agctattttt gcttggctgt
    24541 tcttattact tgatgcagga cagctttttc acaatctctt tttcaatacg attccagtat
    24601 ataaccatgt cgtcagtaca tagtagatga aatacaatac gaagaatatc gtaaacggaa
    24661 cccaaatatc tttgtatgga ttttgcatga atagaccaaa catttgtaat ccgaataaga
    24721 catggactac acctaagatt cctggtgcta agaataacac gcctacttct ctacggatcg
    24781 attgtttaag caacccgcgt cgtgttccta tcttttcaag catttcataa cgagcaatgt
    24841 cactcttgct tcctgacaag atcttgaaca tcaaacagct agcaagcatg gtcaagaaag
    24901 ctaaacctag gaagaatccc ataaattcaa aaccagagaa tagaccatta tacacttcat
    24961 acatttcata tttctggttg ctgtactcta ctttttgcaa agatggattg ttcattttat
    25021 tttgagcaac aagtgtttcg attggtttca ggtttgcttg gaagtctttt acctgaacta
    25081 gctgtaaagt ggattgtggc aagttcagtt gatcaaattc tgattgactg actaccttga
    25141 tttctttttc ttgctgttcc ggcaattcat agcttcgaag ttcctcttgt acagtcagtt
    25201 cattcatttt tttagcagga actttttctt tcgtgatctt cggcggtgtt ccatcttcat
    25261 gttttatcat gatcaatggt tgagccgcaa attcacttgc attatagtaa atcgttcccg
    25321 catcttcttt ttgagtataa gaagcagaaa gtgttggtga aagttctttg attttttgct
    25381 gatcttctgc ccgaggatta ttcatcacta gatcatatcc agaattgttt tctgtcaagt
    25441 ctgcaatttc atttttaaaa cctaatccta ccgtcaaagc gcccagtgcc aaggcaaaca
    25501 gcatagcgac cattgagagc atttgtgtat attcacgaat acgaaagttt aactgtgaca
    25561 atgtgaaatt gttcaatttt ttcaatgaaa tctgttctga tttctttagt aatgataaaa
    25621 agaaaatgat cacagaatgg aaaatgaaat aagttcctaa tacgatcgta actaaggcta
    25681 cccctatacc aaagacttga taggtcccca cgtgtttcat catatagtaa ccgcagccta
    25741 gaaaagcaat tcccagtact acttcaagga aaaacaagaa tttatgctgc ttgattcttg
    25801 ttggtgtctg atccgcacga atcaatgcta aaatcggttt tttgacaatc gaaaaagcat
    25861 tgacaacagc agctaataaa aacaaaacag caaaaaacaa aacagtgatc aacaacccgt
    25921 gaatactaaa aggtgtaaaa tgcgaaatat tgagatctaa ctgatccact aataatcggt
    25981 ggacgatact tgtcaatcct acaccgacag ctgaacccac taaagtagct gcgatcccta
    26041 ctaagaatgt ttcagaaaaa atcatctgtg cgatttttct gccttttgct cccagcatca
    26101 taaacattgc atagtctttt tgccgcatcg tcatcaaaaa ggaatttgca tataaaatat
    26161 aaacgaatgt gatgatccct agtaaaacag aaccgaatcg gaaaatgata actgtagccg
    26221 caacgatcgt attacttttc aaaaaagctt cgttgcttgc cattgattca aacatgtaaa
    26281 aaatagcaga tgccataact aatcctgaga ataacaccaa gtagtctctt aaacggctct
    26341 tgatcccagt aagtgataat ttccaaagca tagtcaatct ccccctactg ttccaagttt
    26401 ccaagaatag atagtatttc tcgatagaag gaatcacggc tttgatctcc acgtttcact
    26461 tcttgatgga taccgccatc cttgatgaat aaaatacgtt ggcaataact tgcagaaaac
    26521 ggatcatgcg tcacaagaag aatcgataca tgatcttttg tattcaattc gtccatcgta
    26581 tctaaaagat cccgggcact tttggaatcc agcgcacctg taggctcatc ccctaataga
    26641 atcgttggtt gtgtgatcaa tgcacgtgcg gcggcgaccc gttgtttttg tccaccggaa
    26701 atctcagaag gatagctttc caaaatatga gagatggata aacgttctgc aattttttgg
    26761 acacgttgct tgatttcttt tggtctgacg ccttgtaaag aaagaggaac agcgatattt
    26821 tcttgtgctg tcaagctttc taataaattg aaatcttgga aaataaagcc aatctcattc
    26881 gcacgaaaat ctgctagttg atttcctttt aatgtggtca catctttttg attgattcgg
    26941 atatgcccat ctgttggttt atctaatgtg gataaaatat tcaaaagtgt cgacttacca
    27001 gaaccagaag cgcccatgat cccaataaat tcgccttttt ctacatcgaa gctgatgccg
    27061 cttaatgctt gcgtcttttt ctcattgttt tttccgtata cttttgttac attctgtact
    27121 tcgactattt tgttcatttc tttccctacc ctttaattag tttttccagt gctgttttcg
    27181 gcacgtcttt tttttcgact tcttcatacg aagtgacacc tttatttttg ttccaggtga
    27241 tttttaaata tgctccttta cgaagcggtc gagtcaattg tcccttgaag tccagttctt
    27301 tttccttacc ctctttcgta tagccaggca ggatatatct atagttgact gtttcttgtc
    27361 cagagtctga ttttgaaact gtttttgtac catctgtcgt aatttgtaca tagtattctt
    27421 ctccacccat aactacgtaa tctgcgattt tcacccctgc aagtaccaca acgatgaaaa
    27481 caagtagacc gatcagaagt tttttcactt tttccccttc tctcttactt tatatactga
    27541 ttatagaaaa aagcgaacaa ttctgccttc gaatcaacta tcattcttct ttcaattttg
    27601 tcattttctt taaaacaact aaaaaaagag ttcaagacaa taatccgtcc taaactcctt
    27661 cttcctatct tacaaaaagt atgtcactta cggtctttca tgtcgcctgt attgataaca
    27721 ggttgcgttc ctttgtctgt aatgattgag acaagtaaat tattgatcaa ttggactttc
    27781 cgatcatcgg tgaaattgat ttcttgtcca tcttcgattt gctctaaggc catttgagtg
    27841 attgtgacag ctccttcaac aatggtttgt cgagcagaca aaatggcttt tgcttgttga
    27901 cgttggagca tggcactagc gatttctgtt gcataggcta aatggttcaa gcgtgtttcg
    27961 attacttcga cacctgctac tgctaaacgc tcttgcagct ctttttgcaa ctcgtcagac
    28021 actgccgtcg tgtttcctcg aagcgtcaaa tcattctcat tgaatgtatc atacgggtat
    28081 tggctagcga tatgccgaat ggctgtctca ctttggattt ctacaaaatc ttgataatac
    28141 gctacatcga ataatgcctt tgctgtatca acgactttaa agactactac tgctgagatt
    28201 tcgatggggt ttccatccaa atcattgact tttaaaacag aactattgaa attccgaact
    28261 ttcaacgaaa cagtcatttt ttgtgccaaa ggtatcgtaa ggaaaaagcc attttctcga
    28321 atcgtaccta aatattgtcc aaaaaacaaa attacttttg cttgattagg actgactaca
    28381 gttgccgaac ttaacaacaa taacgtgatc aaccaaagaa aaatacttaa aaaaagtgcc
    28441 caaactttcg cttgccacat acctaaatag aagaaaaaca atccaactaa agctaatacg
    28501 accaatccga caatccctgc ataaccattg atataaaatg ctcttttttc tctcatgcta
    28561 cttcctgcgc tcctttatcc ttcaataaat atctaatgtt acttttttaa tttttttttc
    28621 gaatacgcgc cattacacct tctagttcta actctttgtc ttcagtgatg taccaaccgc
    28681 ttgcctgtgt ccctccggct gcctgcggta aataatccaa ttttgttcga acccagtgct
    28741 tttcttctgt aaaatagtct tctaccaaga cctctagatc catccctgct gaaagaggca
    28801 aatcttcccg aacagattta aagataaatc gttccttctt cccatcatac ttcaatattc
    28861 ctgtatcgat aatcggtaaa gaataataat ccagctcttt cttcaccgtc aataaatgtt
    28921 tgaaaacttc atggtacact tgctctaatt ggcgaccttc tgaattgttc gggtaattaa
    28981 ccatcacttt ttctttgtct ggatcataga aggtttggct tagttgagtg atataagtat
    29041 gtaatctttc catttctgtt tttaactttt ctctttgacc ttcttctacc ttcatatact
    29101 ctttcaccct gttttctttt tagatatttt ctttcttatt ttactatagt tatgtgcgtt
    29161 aagcgatgaa aaacagcaaa gattggaagg atctttgctg ttttttctta aaatatccag
    29221 ttgagtacag gatcaatctg tttcattgtc ctcttgatcc gtcgtaatat tgatcgatgt
    29281 gtctttagca gtgatcttta gctttttctg tccttgttct aacaaagtat ttgttattgg
    29341 ctggtagtct ttttcaatat aagattcacc agacgtttgg atagtatagc cactgttttc
    29401 catctcatgc aaagataaat tgccaccgtt taggatgact tttccttctt tgctgatcaa
    29461 actgtcttct agatggaagt tgctgttttc acctgtgacg atcaagttat ccacattggc
    29521 ctcatctaat cgaagtgagc ctaattcatc tttcgcagtt attgttcctc ctgtaaattc
    29581 attgaacgat acccagccat catgtgtctt agtattcact tttttgattg atacttcgtt
    29641 gatatcaatt gcactattat tcccttcgat tgtcaattca gataattcat gatctgcagg
    29701 gacaacaata gtgatttgtg cacttgtgtc ttctaaacta atgattgcac ctgttgattt
    29761 tttgactgca ttcacagtca acacgccatt tttctccttt atttctggct tttctgtctt
    29821 atcataagtc gtttttatct caaaatccaa tccgcgtatg accgaaactg tttgattttc
    29881 tgcatggatg acgattttgt ttgcatcctt gatttttttt gtctcagtaa ccatatcgat
    29941 caaccgagga ccattatccc aagtcagact cttttgagcg cctaatgtat aagttgcacc
    30001 tgctaggagg ccaccagtaa gcatcagacc aataccggta atcaaaaaat gtttcaatct
    30061 caatttatgt gtcctccttc aaacgcccgc tgaatgcaga tctctttttg taataaaacg
    30121 tcgtcccatc catttaacaa agttcatcat gacataaaaa agcccttttg ttcctttcag
    30181 caacagtggc gaaataaata ctgcgcttcc tgtggcgaac agaccgacac cgataaagaa
    30241 taatccactc agaatcgatt gtccaaacac actgattcct gagatgatca tgaataggcc
    30301 aacacctaag atactgatac ataaaacata gatactaaaa atgagcgcta gtaaagcaac
    30361 caatagtccc ccaataacca atataaaccc cagtgctagt ggaatgagga caggagaagc
    30421 aaataaaccg ataatgatta accaaaccag gcgaatttgt ctttttggat ttttcactaa
    30481 ctcgttttct gcttgttctt cttctgaaaa gtaactgatt tttaatttta aggcaaactg
    30541 ctttggtgta ccatattcat caatgatctg atctaatgtt tttccagaat ctaaaaaata
    30601 ttcctcataa tactggatca aatcaaaacg atcgtcttca ggaatttctt gaagatgatc
    30661 cacaacttca ttcaaatatt cattcatttg tgctctcctc caataaccgg tctaaagaat
    30721 ctcgaaaaac aatccattca tcaataattt cttctaaatg acgacttccc attggtgtca
    30781 acgagtaata tcgacggttt ctgccttcaa aaggttcatc ataggtctca agatatcctt
    30841 cttttttcaa tcttctcaaa accggatata gtgtagattc actgacagag agactttctt
    30901 gtacgatttt tgttaaggca tacccataca tatcttcctt gcttaatgct gctagcacag
    30961 taccatcaag aagaacagtt ggaatctgga tgtgcattac gtcaccttct attctcaagt
    31021 gtaatatttt atttcaatct atattataca acatataata tagtttcaaa atcagtttgt
    31081 caaatacaaa aaaactgact aaaagatttg atccttttta gtcagcaaaa aatggcttct
    31141 ctttgatttt tattataaca gtggcataat ccaatttgtc cacatccagc tcagtggcat
    31201 gataacgatc attgcaggta tcatgtcagc tactgggaac attttcacgt tgatcattct
    31261 aaaaccggta gcgaccatca agaatccgcc gcaagcttta aaatctaaga tcatgtctgg
    31321 tgtagtcaat ggataaataa attttgccaa taagaacaaa atataaaaaa taatgaattg
    31381 gggaattgct atcacagaaa cgacataccc taaattacac gcaaaaatag ctgctgtgaa
    31441 aaaatcaagg acagatttag aaatcaaaat cgtactgtct cctgtcatcc cagaatctaa
    31501 acttccatag atccccgtac cacttgcaca aaatagaaca atgaccgtca ctaatgtgct
    31561 gataaattca tcgtgggtca taccaagctt ttcactaggg aagattttag aaataggtac
    31621 ttgcatcagc gcagccccct tatttatccc attacctaaa tgaacaatca aaccaatcgc
    31681 tgttcctatc actaaagcaa aaataacagc cggcatattt ttcatcggcg caatggaata
    31741 gattcccatc cccatcgaac acacaccaaa aaccatcgtc agctgtgctt taaaatgttc
    31801 acttaattta tttcctacta atccgccaat tagtccgcca aacaaaacgg aacctacatt
    31861 gatcaaaact ccagttggca tcaaaaaact cctctctttt tctacgaagc gaaaaaaatc
    31921 agaaaaattt ctgtaattat tgctttcgct gcaagaaaga ttgtaacaga cagagaagat
    31981 tacaccaatt cattttttgt gttaaagtat tctttattgg aataagtagg aggaatatga
    32041 tggatatcca taaattcgaa gtttttcttg atttagccga aacaatgaat ttcaccaaaa
    32101 ctgctgatcg acaattcacc acccaaggaa atatttctaa acagattctt tcgttagaaa
    32161 cagaattaga cgtcaaatta ttcgaacgcg cccatagaaa aatcgaattg accgaagctg
    32221 gaagcttatt gctcccatat gtaaaaaatg tcgtagaaca ataccatgct atgcaagaag
    32281 tgcttgaaac ctatacgaaa gacaacaatc tttcattaaa tatcttaacg ataccgacaa
    32341 tgatcaatta taaaggtttt tccaaaatca ccgagttttt gaaacgccat cctgaattta
    32401 cagtccagtt gaaagaagtg gaaagtgtcg aattaactta caatgacgcc aatgaacata
    32461 gtgacacgat ccactttgct agaagtttcc aggccccacc aagttcgatt gagtggttgc
    32521 ctactgaaac agacgatttt gtagcagttc tacctaaaaa tcatccttta gctagtaaaa
    32581 agaaattgga tctaagcgaa ctgaaaaagg agaacttttt gctccttggc cccactacga
    32641 acttgtatca acctgtacta gatctgtgtc gcgaagctgg atttgaacca aaaatctctt
    32701 ataaaggagc aagaatcgac ttgatcatca atatgattgc aaatgacatg gggattgcta
    32761 ttgttatgga aaaaacagtc aagaatctat taaaagaaaa tactgtgatt ctccctattt
    32821 ctccaacaac agagagttat cttgcattta ctcgtaaagt cggtgagcat tctttggctt
    32881 cagatacatt ttgggagtat ttaaatcaag aagaaaacta agccctacat tttctgtcca
    32941 tctatccacc caaaaaaaga gagcgacgca tgactttcgc tctctttctt gcatcatgtc
    33001 cttgtttcaa tgacacctct aacatttctc gtagttcaat aagaaagcag cgatttttat
    33061 gccaaaagaa ccgtaagaac atttccctaa tcacaaaaaa acgactaaaa acccctaaaa
    33121 accttcaata gaatttttag gggttttatt ctttttccta ttgtttactt aaccgtatca
    33181 ttgctgtcgt tcctactcct tcgatactgc tataatcaat acgtccatca tgcagttcta
    33241 gaattttttt aaccagtgaa agtcctagcc cattcccaga aatttgacga cttgtatctc
    33301 cttgataaaa tttttcaaac attcgatctc tggtctcttc attcattcct acacctgaat
    33361 cggtcacttc cagtacaatc tctgtggctg tctcaaatag tttgatatga atctgtccat
    33421 tgatttgatt gtactttatg gcgttatcca tgatattcag ccacacttgg tacaaaaact
    33481 cttcattgcc tgtgtaattt actgcagcaa gttcgatatc caattctagc tgttctttct
    33541 cccatttagg ctgaaaaaaa acgattactt ctctgatttg ttcatctaat cgatactctt
    33601 tcttttccag ttggatacgc tggttttcca gtttgttcaa ttttaagatg ttctctgtta
    33661 attgtgaaag ctgcgtgata ctttcgataa tccgatataa aaatatttgg cgttcttcgt
    33721 cagagagatt tggggcttgt aacaattgaa catatccttg gattgttgct agtggtgttt
    33781 tgaattcatg ggatacactg gacacaaagt cattcctcag tgtctcgata ctattgagtt
    33841 cttggaccat aacgttgaaa tctttataca gttgctgtac ttctgctact ttctgctgtt
    33901 catccattcg aatcgaaaaa tctcccgttg ctaccagact catatttgtc cctagcgcac
    33961 tgattggatg caggatacgc cgcccaacaa aaatcgatat tcctgtaccc acgaccatac
    34021 tgaaccccgc aaagacgaat aacgggtata acgttggttt tccagccgat tcagcaggcg
    34081 agatagaatg tcgagacaat aagaaaataa aaccgagaaa acacagcagt gtcactaaga
    34141 tcgaaatgaa gacaatacta gcaaaataga tccataattg tgaaaatagc ttttttttca
    34201 tctttaaata accgccttat agcctagtcc ccgtactgtg atgatttgaa aatcttccgt
    34261 cttttcaaaa cgggtcctta aacgtttgat atgtacatcg atcgttcgtt cttctgtatt
    34321 cgtatctaat ccccaaatat catccatcaa ctgttggcga gtgaagatct tgttgggata
    34381 agacagtaat ttatacagaa gcagaaattc tttttgaggc agaatcaacg aatgctgatc
    34441 ttgtaacact gtataagcat ctagctctaa ttttgtttgt ccgattatca agctactgct
    34501 gtaattgatc tttgaacgtc gaagcaatgc ttccacacgt aggatcatct cattgacatc
    34561 gatgggtttt gtcatataat catctgctcc taatttgaac ccgcgtttct tatcctcaaa
    34621 atcggccttt gccgtaatca ttagaacagg tgtgtcaaat ctggtgctac gtaatgagtc
    34681 tagcaagtca tagccatcca ttttcggcat catgatatct gtaatgatta attctatacg
    34741 tgtcgcttct agttttttca gcgcatcctc tccattcaat gcggtgatga ctgaaaagcc
    34801 cgctcgttcc aacacagaac ggtaaagcaa ctgtaattgc ggatcatctt ctactactaa
    34861 aatacgattc attgatctcc ctctgttttt tgaaaaattt ctagattttt tcttgtagtt
    34921 catcttcagt taacatttat ttcgtatact acgatcattg ttctgatata cttattagta
    34981 taccgaatat ttcaatacgg gagaagggtg attatgcttc aattaaaaga tataaaaaaa
    35041 tattataaag tcggcgaaac caccaccaaa gcattggatg gtgtgtctgt cgctttcaga
    35101 caaaaagaat tcgtagcgat tttaggtcct agtggttctg gtaaaacgac tatgctgaat
    35161 gtgattggtg gactagataa ttatgattcc ggtgatatgg taatcaacgg aaagtctact
    35221 aaagacttta aggacagtga ctgggatgcc taccgaaaca actctatcgg tttcattttt
    35281 caaagttaca acttgattgg tcatctaggc attattgaaa acgttgaact tgggatgaca
    35341 ttaagtggtg tttctaaaga cgaaaaacga aaacgggcag aagatgcatt gcaccgagtc
    35401 ggcttgacaa atcatatgca taaaaagcca aatcagttat ctggtggaca aatgcagcga
    35461 gtggccatcg ctcgagcact agccaatgac ccagatatcc ttttgtgcga tgaaccaacc
    35521 ggagcgttag atacagaaac aagtatccag atcatggaat tgatccaaga gttatcgaaa
    35581 gaaaaattag tgatcatggt cacacataat cctgaactgg cccatcaata cgcagatcga
    35641 atcattgaat tttctgatgg taagatctta acagattccc atcctcacat cgaacgacca
    35701 aaagatgatc aatttaactt acgcagaaca aagatgagtt tttggacagc gttaaagctt
    35761 tccttcaaca atatccgaac aaaaaaagga cgtactttct tgacctcctt tgcttccagt
    35821 attgggatca tcggaatcgc gatcgttctg tccctttctt ctggattcca aaaacaaatc
    35881 gataacacgc aagctgaaac gatggcgaaa tttccgatta ctatttctaa ggtaacgact
    35941 aaccagactc gtgacgatgc cggattgggt gcaagcaaag cggattatcc agacagcaaa
    36001 acgattactg caaaagtcag cgaagaagat aaagcacagc acacgaataa gattgatcaa
    36061 acatacgtcg attacgtaac agacatcgat cctaacttaa gtaataatat cggatttacc
    36121 cgtactacag gaatcaatct tttacgtgat gtaaatggca aagtacagcc tgtcagcttc
    36181 tcgaatcaaa atcctgatac ggaatctctt tctttatcta gtacgatgtc agcaatgaca
    36241 ggtgtgggag tgtcaagttt ccctacacag cttgacacta gcaaagaaaa ctttttaaaa
    36301 gacaattatt ctttacttgc cggaagttat ccagcctcag caacagatgt cgtgctgatc
    36361 gtagacggca ataataatac taatatcaac gcattgaaga atcttggatt tgatgtgaaa
    36421 gaagacgaaa aacttgattt tgatgatatc gttggtacaa cattcaaatt agtaaacaac
    36481 aatacttact atacaaaact tccaactggg aattttatcc ctaatacaga ttatgatgcg
    36541 atgtatcaga atgcttctga tgaattgaag atctcgggga tcctacgcgt gaaatcttct
    36601 tctacaatga atctcttatc tccaggaatc gcttatagcg atcaattaac gactcaaatc
    36661 gtcaacgaaa acaaagaatc agaaatcgtc aaagctcaaa gagacagtgg tgtaaatgtc
    36721 ttaactactg aaaaagttga cgaaaatgcc aaacaaacat tgcttagtta tttaggtgga
    36781 gacagcttgc cttcaagtat catgatttat ccgaacaatt ttgaagacaa agaaaagatc
    36841 ttggattatc tagatgatta caataaaggc aaatcagatg aagataaaat catttatact
    36901 gatttagctg gaacgatgac tgaactgact ggcggattga tggatgctat cacttatgtc
    36961 cttattgctt ttgcagggat ctctttagta acaagtatga tcatgatcag tatcattact
    37021 tatacatctg tgattgaacg gacaaaagaa atcggtgtac taaaagcttt aggtgcacgt
    37081 aaaaaagaca tcactcgcgt atttgacgca gaaacgtgta ttttaggtat ttcttctggg
    37141 atattaggtg tgtttattgc atggctagct acgttcccta tcaatagcat cttatacaac
    37201 atgacggatc taaaaaatgt tgctcaacta aatcctgtcc atgcgattat tctagtgatc
    37261 gtctctacta tattgacgat gcttggcggt catcttcctg cacgaatggc agcgaaaaaa
    37321 gacgccgcta tcgctttgcg tgcagaataa ggttgtgaca accacattta gatttgagtc
    37381 ataagagcgc tgaaatagta gaaaacagca agaaacgagg aagtaatatg ttccttgttt
    37441 cgcgctgttt tttttaatta ttttttataa ttctggtata cttttctttc ctttgtacca
    37501 caactcttct ggcgtatacc tgtcttttcc atttatgaat actgttccat ctgcttcaat
    37561 cgttacttca gtcccaatcc acatattacg agaaagtgcg agaagatttt ttctttttcc
    37621 tcttcctttt tttccaaatt gcaaggcatc ggttatctct tctgtccaat gagatttcac
    37681 gtattttact tctccgtttt caaatgtaac tttcaaaata ttcgctatat tttccacttt
    37741 tactgcttta ggaaatgact cctccatttt tttcactttc cttcctccaa cactaagtat
    37801 gcgctttctt ttagtgataa gtatatcata gtttattcta ttatcaaaaa aacgcatcca
    37861 ttccattgta gaatacttta ccgaacaagt tgaattgttt tcctcttttt caccttttac
    37921 tatagatagg taaagacaac taaggaggaa tctctcatga cagatcaacc gtatgattta
    37981 agaaaagtca tcgaagaact aaaaacactt ccaggtcaat atcacgaaac agatattgaa
    38041 atcgatcctg aagcagaatt atctggtgtt taccgttata tcggtgctgg cggtacagta
    38101 aaacgtccga ctcaagaagg accaactatg actttcaaca atatcaaagg tttccccggc
    38161 acacgtgtga acatcggaac aatggctagt cgtaaacgtg taggtgaaat tttgcaccat
    38221 gattatcgaa cacttggtcg tttcttgaaa gacgctgtag aaaatccaat caaacctgtg
    38281 aaagtagaca aagcagacgc tccagcacaa gaagtcgttc atctagctac cgatgcagac
    38341 ttcgatattc gcaacatttt gcctgcacct acaaatactc ctgaagatgc tggtccatac
    38401 atcacaatgg gtgtagtctt aggatcagat ccagaaaaaa caatgagcga tgtaacgatc
    38461 catcgtatgg ttctagaaga taaagatacg atcggtatgt acatcatgcc tggcggacgc
    38521 cacattggtc acttccaaaa acaattcgaa gccttgaata aaccaatgcc tatcactatc
    38581 aatattggtt tagatccagc aattgccatt ggcacgactt tcgaaccacc taccactcca
    38641 cttggctaca atgaactatg gtgtgcagga gctttgcgta acgaacctat tcaaatcgtt
    38701 gatggtatag cagtcgatga agtgggtatt gctcgtgctg aattcatcat tgaagcagaa
    38761 atcatgccaa atgaaacaat gcaagaagat atcaacacga actcaggaaa agcaatgcca
    38821 gaatttcctg gctatactgg ggatgctaat ccagctgtac aagtagtgaa agtcaaagca
    38881 gttactcacc gaaaagacaa tccaatcatg caaacaacga ttggtcccag tgaagaacac
    38941 gtatctatgg ccggtattcc tacagaagca agtattcttg ccttggtcga taaagctatt
    39001 ccaggaaaag tagtaaatgt ctacaatcct cctgcaggtg gtggtaaatt gatgaccatc
    39061 atgcaaatcc gtaaagaaaa cgaagcggat gaaggtattc agcgtcaagc agctattctt
    39121 gctttatcag ccttcaaaga attgaagaca gtcattttag tcgatgaaga tgttgatatt
    39181 ttcgacatga acgatgtagt ttggactttc aacactcgtt tccaaggaga taaagatatc
    39241 atcgtgatcc caggaatgag gaaccatcca ttggacccat cagaattacc agaatataat
    39301 cctggctcta ttcgtttccg tgggataagt gcaaaaacta tctttgacgg tacagttcct
    39361 ttcgatatga aagaacattt tgtacgtgca aaattccaag aagtagaaga ttgggaaaaa
    39421 tatttgaaat aatgaatgat ctgcaactgt tttgacatca taaaaaggag tgcggaaaat
    39481 ggaacaattg gcacaacgga agaaaaaaat cgtgattggt gtaagtggcg cttcaggcac
    39541 catttatgcc atcaacctag tgaagaaact aaaagaatat ccgatgatag aaacacacgg
    39601 aatcatcagt ccatgggcaa aacagaactt aaagctggaa tccgacttat cactaactga
    39661 attcgaaagc ctgtttgatt accattattc aaacaaagat ttaggtgctg ctgtcgccag
    39721 cggatcattt ttgaccgatg gaatgatcat cgtaccggca agcatgaaaa cagtcgcagg
    39781 tatctctgtt ggtttaggcg acaacctgat tgcacgtgct gctgatgtaa caatgaagga
    39841 gcaacgaaag ttagtcattg ttcctcgtga aacgccatta aatgccattc acttggaaaa
    39901 cttgactaaa ttagcacgac taggtgtgca aatcatacca ccaattcctg ctttttataa
    39961 tcagcctcaa acgatccaag atcttgttaa tcaccataca atgaaacttt tggatcaatt
    40021 tgaaattgta aatgagcatt caaaacgttg gcagggtgat taatatggaa aaagaaacga
    40081 tagttgaagt aaccaaacta ggtttcacga tccaagctgc tgttaaagta atcggtaaag
    40141 acttattgat cactttaaca ggcggagata caccacatat cggaacagtc actacatttt
    40201 ctcgtgatac agagatgcaa actgtacgtt tccctagcca cgatggtcgg ttccataaag
    40261 acgatgtctt agccgaaaaa atagctgtga tcatcaaaac tgccgttccg ggaagttgta
    40321 cgatcacttc cggtatccat gttgaccata tctccaatgc acaaatcaac gcctcttttc
    40381 cgatgtcaga agagttaggt tacaagattt tagattggtt ggaaaaaata gatttccata
    40441 tcaaagaacc agtttattat aaagatggtg aaaaaccatt atgaaagaag aggttgtgaa
    40501 atcagcgtta tgacctgagt atgagagaaa aaaatggaaa aatagcttct catattttgc
    40561 cgtttttgag ctaataccga gggacagctg attgaacgcc gtttatcaag aggttgtgat
    40621 aaagccgctt cgtttcgagt agtaagaaaa attttgtaaa catgggtttc cacatttttg
    40681 cataaatttg acttactacc gagaaactgg cttttgaaca ccgtttatca agaggttgtg
    40741 acacgtcttt tgtcacaacc tcttttattt ttgcttcact gtaactgcta caatatcacc
    40801 gatttctttt ccgatgtttt tacgaatatc ttttcgtagt ccaaggatgt agcagactga
    40861 accatctgga tttttcacgc ccatattcac gatactgcct tcatatggca cattgtcaaa
    40921 agttgcttgg actttaactc ttccagcacc gaattctttt cttatatcat acggaaaaat
    40981 aatatatgcc ccgcccttgc cgacttcgga agcatagatt tttgattcaa aagaataaat
    41041 ttccctcatt ttatttctcc tattttttac atctagtata gcatagcaaa aaaaagaagg
    41101 aactgtgaca aaagtcttgt cgcagttcct tttttcgaat aaacggtgtt ctcctgccaa
    41161 ctcctcggtc acaagtcaca atagtcaaca aacatgagaa gctgtttttt gaaattgctc
    41221 cttgtgactc agagttaaac ggcaggctca caacctcttc cttaaacggt gttcaaaagc
    41281 ttgcatctac ggcactaagc ccaatcagat gaaaaatatg gagtgctatt tttatctgat
    41341 tgttcttact gcttgatgca gagcagcttt ttcacaacct cctacatggt gttcaaaata
    41401 ttattcgcct tttgcttctt tcactaattt tgcgaatgct tgttgttgga attcagcagc
    41461 taaggctttc gtacgttgtt cttgctctgc tttgctcatt tttggatttt cagtcattgc
    41521 ttttaccata ccgtcttgaa cataagcttt tgcttcactt tccatcgtac tttgctgttt
    41581 cttcactaat tcagctaact tggctgcttg gtccgtactt aattctaccc atgtctctgg
    41641 gattttgaat gtattcactc ggctgtcata ttcgtgattg ttatcagcaa tattgaacca
    41701 gaatttatac atgtcgtttt tgtattccca ataagtagtt ttcgtcactt tttcagcatc
    41761 gccgtctgtc ttttctactt tatttgtttc gttatccgta ccagttgctt ttggtttttc
    41821 ttgtttttca tttgttcggt aaagatacac tttttctttt ccattcccta aagctttata
    41881 caaaagcata tctgctcctt gggaatcagc actggaaaca attttttctg tttttgtttc
    41941 cgttactttt tccattccaa agtgattgga caaattagcg acgatcaaac ttagtgaagc
    42001 gacgaagatt agtccgaaaa tcaaagataa aaaggtttgc catgtttttt tagcgaacac
    42061 gttcgttaaa gcaaaagcaa gcaagctgac aatcaaaact ccaataatca tgcattatcc
    42121 cccgctttca ttagaacagg ttcttttttc ttcaataaaa aggcaataat aaagccaaga
    42181 atcccaaaga tgatcgctac gataaacgcg gcatggtaac ctgacagtgt ggcattgatt
    42241 gcctgatctt tataagctaa tggcgtatta tccaatacag atttgcttgg gatatttgct
    42301 tttgtcacat tcgtcaacac actgatcaaa atagctgtac caatcgagct tgcgatttga
    42361 cggaatgtat tatttaccgc tgtcccatga ctgatcaagt tatttggcaa tgcattcata
    42421 ccagaagtcg ttaccggcat catgaccatg gagatcccaa acatacggat cgcataaagg
    42481 atcacgatat ataaaactgg agtcgttttt gtcaagaaag caaatggtgc agttgccgct
    42541 gtcaaaatga acatccctgt gatggctaaa cgtttcgcgc cgtatttatc gaaaatcgca
    42601 cccgtcacag gcatcatagc ccccatgatc aatgcacccg gcaacaacat caagccagaa
    42661 tggaatgctg attctccacg aatattttga atatacaatg gtacgaccat ttctgcccct
    42721 accatcgcca tatttgttac accagctaaa acagctgcaa tcgtgaatgt agatgatttg
    42781 aatacacgaa gttccaagaa tggatgttcc aaatgtaatt gacgccatga gaacaaggcg
    42841 atgaaaatca caccaacaac taagaaacca attactttca agcttcccca gccgtcatct
    42901 cctacactag aaaatccgta aagtaaacta ccgaatccaa tcgttgataa aatagcggat
    42961 aaaatatcaa tgctaggatt tgacaatgga ataacacttt tcattaggaa gaatgctaaa
    43021 accaagacaa gaacaacgat tggcagtacc ataccaaaca agtcacgcca atggtagtta
    43081 tcaatgatat aaccagataa tgtagggcct aaagcaggag caagtccaat aacgatccct
    43141 gacatcccca ttgctgatcc tcttttttct ggcgggaaaa tagatagcat gatattttgc
    43201 aataatggca tcgatactcc cacaccgcaa gcttggatca aacgaccgat tagtaatgta
    43261 ctaaaattag gtgctgtaaa gcaagtaatc gttccgatca aaaagatcgt catggcagaa
    43321 agatataaat ttcttgaact gaatttattg atcagccatg ctgttattgg gatcatgatc
    43381 ccgttgacta gcaagaaacc agtcgttaac cattgcacat cagacgctga aatatcgaaa
    43441 gctttcatca atgtaggaaa agctgtcgtc aataaggtct gattcaaaac ggtacaaaat
    43501 gtaccaatta ataagacaac aactaaaagt gtccgattat atggttttcc gtaaatatct
    43561 acgggttggt tgttactcat atttctcctt ctttctgaat tttttctgaa actactagaa
    43621 tactttagtc ctaaattcgc tatttgtcaa cctacttgac acataagtta atacggatat
    43681 aattaaggga agcaagagga caaaaggagt gacaaaaatg gttggggctt cccttaaacg
    43741 tttgttggaa aatgatcaat tggttgttgg catcagtgaa ttaagtgata tcgtaggtgt
    43801 gtctccacgt caattaaggt attgggaaca aaagggattc attcgttcga tcgaagctga
    43861 tgcgaattgt cctagaaaat atcgtttacc tacagtcata aaagtagaac tgatcaaaaa
    43921 atatttagat gacggattta ctttgtcaaa agctgcagaa aaggcagaaa tccgccaaaa
    43981 gaaaatgcac catgtccgta aagttttttc aaaagcaatc aaagatatcg aattgatcga
    44041 cgaacgctat acgattttac aaatcggtaa cttcaaagaa gagaatgcta agatgtacat
    44101 cattcatgac aacgagacag acgaattaac ttacaagctc cttcctgtcg atgaaaaagc
    44161 tgactacgaa cagttgttaa aagaactcta aacgccacta atctcctaag taccaagctt
    44221 tttaataagt agatgctaca taaatacttg aaaaaaggcc tgtttgaaca taagaatatc
    44281 cgaacggtga tctgcgtttc tttcatatag aaaggaattc tccatcactg ttcggatatt
    44341 tttttatttt tttcggttac tttcttctaa tacatctgtt agccaatttg catattctgt
    44401 ttcccgattg atcgcatgcg ctaaaatcag gtagtgtccg taattttcat gtttttcttt
    44461 ttcagtagga aagatcgttt tttttcgctc taatagatga agtcgttttt cttcatgaag
    44521 gtcacgctgc tgctgaataa tttctactaa tgccggatca ttcacgtctt tgacgaaata
    44581 caatttcaga acaaattcgt ctcgattaac cggtagttca ttggtgggtg tatgaatcca
    44641 ctctgccaat tgttccttcc cttcttctgt taattcataa actttctttt ccaatttttc
    44701 acctgtgatc tctacttgat gctgaatgat tccttgctct tccatttttg caagttcagg
    44761 ataaatctga ctatgttttg cttgccaaaa ttctcctatt tcatttttga acgattgatt
    44821 caattcatat cctgtcatct tcttcttact taataaccct aacaaaatat atcgaagtgt
    44881 attttttctt gccatcagtt ttccctcctt gacaaatctt taaatccatt ctatcataaa
    44941 tgagtaatcg aaaacatgta gtttattaca tgttttaatt tataaggaga tgcttaaaca
    45001 tgaaagaatt taaagaattg gaagatttta ttggaactca ctttatctat acgtatgaca
    45061 atggttggga atatgaatgg tatgccaaaa atgaccatac tgttgattac cgtatccatg
    45121 gcggaatggt caaaggccgt tgggtaaaag accaagaagc tgatatcgtt aaattgacgg
    45181 acggcgtatt caaaatcact tggacagagc caacagggac agacgtggca ttggacttta
    45241 tgccaaatga aaagaaactt cacggaatga tctttttccc taaatgggtt gaagaacatc
    45301 cagaaatcac tgttactttc caaaacgaac atatcgcaga aatggaagca gctcgtgaaa
    45361 aatatgaaac ttatccaaaa ttacttgttc ctgaatttgc tacgatcact tatatgggtg
    45421 atgcaggtat agacaatgaa gatgttatta gcgaagaacc atacgatgga atgccagatg
    45481 atatccgtag cggaaaatac ttcgatgaaa attataaacg agtaaaataa ggttgtgaaa
    45541 gagaggttgt gaaatcagcg ttctgacctg agtattagag aaagaaacgg aaaaatagtt
    45601 cctcatgttt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat
    45661 caagaggttg tgaaatcagc gttatgcttt gagtaataag aacgctagtt agaaaatagc
    45721 ttttcatatt ttctaggcta gtgggcttat taccgagaag cagccgtttg aacaccgttt
    45781 atcaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa tggaaaaata
    45841 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    45901 tatcaaagat tgtaacaact gcatattcac acagttaaat tagtgacttc aaaatgaaat
    45961 aatgctccca gcaaaaaatt aggctgggag cattatttcg ttattctgtt catcaaaaat
    46021 cagcgttttg attccatttc atcacaagtt cctcctcatt tactgcttca tctttttgct
    46081 gtgtttgaat tgtaaaacct tgtgaaagat agaaatgaat agctgattga tttttttgat
    46141 agactgtcaa agtcagtgtt ttattggttt cttttatttt tttcattagt tgcgtaccga
    46201 ttctttgttg ccggtattcc cttttcacaa agattcctgc gataaactga tcaatcaccc
    46261 cagcaaaacc aataatttct tgatctattt ctgctaagta aatcactgca tctggtaaag
    46321 ctttcctaac agattctact tgattctccc aataagttga tggaatgaat tcatgagaat
    46381 ccaagttagc atgcagccaa atcgatagaa cagattccag ttctttggca gacaattttg
    46441 ttgttgtgcg gatcattttt tctctcctta cttattcatt gattttaaaa taaaaaccat
    46501 ttttttgaag ctcttttaca agttttcgcc cctcaaaaaa gtatatcttc tcttgtttta
    46561 cttttctaat accaaaagaa acagcaactt taggatcatc agctgaaacg attcgataag
    46621 ttccaccagg caattgatcc agcttgactt tcccatgatg gtcgctttgt actgaaaacg
    46681 gcacaccatt tctagtaagt gtttttttct tatgtttcaa aatgaatgaa ctaccattta
    46741 ccggtttccc tgacgaatca ctaataataa atgaaaaatc aagcactttt ttacttaatc
    46801 tatactgata gattagtcgt ccgataaaca ctaacagtat gactacagct attatcgttc
    46861 ctgcaagaat aaaccattgc cgcatggttc tttcctgatt tccttttcct tcttcttttt
    46921 ttaccgattc gttataagga atccgatgtc ctgtcactag caaacgatgc gtgttgatca
    46981 tatacggcgt acatgtaagc aaggtaacta agtcttctcc ttctgaaatc gttagaacag
    47041 acgtgtcatc tggcagtacc acttgtatat ttttaacttg ataagccaat ttctccccca
    47101 aaacagtcaa aataaacgtg tctccgtttt ctaaacgatc taattcggta aataaccgtc
    47161 gattaggtaa accgctgtga gcagagatca ctgaatgcgt cgaatttcca ccaagcggga
    47221 aagaggttcc ttgcaagacg cctgctccat tttcaaggat cctaccattg agccgatcat
    47281 aaagtgaaat atttaattgg atcttaggta tattgatact tccgatcagc cattcctcta
    47341 attggctgga tgttgtactt tcatttctgt tcttttcatt gaaaggatca gtaccaggat
    47401 tgaatccttg gtcagttaat cgttggtttt tctcctccat tttctttcgc tgcacttcaa
    47461 tttcagctgc ggtccgtttt tgtacttgtt cgatacgttt ttgatcaata aaactattta
    47521 aagaatccac ataaaatgga tataaagcga tcagtaaccc tacgacgata catattaata
    47581 aaaacaaacg aaacaaaagt cgacgagact gtttttgctt actctgcttc acatgtgtcc
    47641 tcccttgcaa gtttttttgt tctttctttt attatatggg ttcaggaaaa aaatagaaaa
    47701 tgaaagccga tgaggttgtg aaaaagctgt cctgcatcaa gtaataagta cagcccaaaa
    47761 aaatagctac tcatattttt tgttgggttg ggcttattac cgcagatgca agcttttgaa
    47821 caccgtttaa ggaagaggtt gtgaaaactg cgttatgctt tgagtaataa gagcgctagt
    47881 cagaaaatag cttctcatat tttctaggct agtgagctta ttaccgagaa gcagctgatt
    47941 gaacaccgtt taccgagagg tcatgaaaaa gctactttgt ttcgagtatt aagaaaaatt
    48001 ctgtttcaaa acaaggagat gcaacaaaac ttatgtcgca tcctctctcc ttagaacttt
    48061 cacttatgtt tttcttgtaa aattcataaa tattttcatg aattttctaa tttcttgcct
    48121 acctctcgca taggatatat gatgaattta tctaaagttt gcaaaggagt tcgacaattg
    48181 aaaaaatatg gcccattttt agcaattttt ttcattgctt tggcatttct catgcctcat
    48241 ttattcacac ataaactaat tttgggtgct gatgccccat tccattacaa tcggttctat
    48301 gatgccgcgc aacaaattaa atatggggat ttccattatt ttccttccct ttatggattc
    48361 cagcaaagtg gcaggatcgt gaatgccgtt tacggcccat ttttcgctta tttacaagga
    48421 ttgatcgtgt tgcttgcagg tagctggttt cattatcaga ttctttcaaa tttcattctt
    48481 tatcttattt caggattttc tatgctgatt ttactgagat atgtcaatgt tcgaaattgg
    48541 gtaagtgtac cagtagccgt tttattcatg agtacgtatt ctattcaata ctggtctatc
    48601 aaccaaggat tcactagctg ggggacttgt ttttatcctt tatgtatgat ccctattgct
    48661 gatttcgtcc ttcataaaaa gttccctgtt atcaaagtcg ctgtctcagt gggacttatg
    48721 acacagatcc atttactgtc ttcgatcatg ttagctttga tgtatatccc tttttatctt
    48781 tattactttt ttaaccagac tgaaaaagga aaagcactgc ttcaactgat taagtcggtc
    48841 ttgctttatt ttgttctgac agcaaatatt tgggcaggat tagctattgt ttacagtggg
    48901 aataagattc ttcctccctt tgttaacaga gaaatgagtc aaaaggcgat agatctggat
    48961 ggcagttatt ggctccatta ccctaaggcc tttcctatat tgatattatc aggttttttg
    49021 ctttttattt tctttcaaca aaagaacacc ttattccaaa aaatcgtgtt gtacacaagt
    49081 cttgttttct tttttctttc tactagcttg attccatgga cgacattgat cgaaaaaaat
    49141 gtgcctttta ttagtttgat ccagtttcca tttcgatttt tcgtgccatt tacgctattg
    49201 cttctacttt accttgccct ttcactgaac cagtggtctg aacaaaagtg gttccgtctt
    49261 ttaagtatat ttggcattct tattttttca gcacagacga tccagaactt ataccagcat
    49321 ctggaaaagt gggaaaatga aacatttgtc agtcgacata cttatctatt cgatacgccg
    49381 gaagaagcaa ggaaaacatt cttcagcaaa gatatgtcta ccagcttgtt cgttttccaa
    49441 aaaaccacac ctgactatct gccaatctat aaggagacaa aagacaataa atacgaccgg
    49501 tataacgaag aaatactaga aaacgaaaca acttttataa aaacacatcc aggcggtctt
    49561 ttgacaatca agtggaaaaa cactggggac aaaactgttc atatccctgt tatcgtctac
    49621 gatcggactg tgctacagca aaatggaaaa acattgactg attatgaagt aacagatatc
    49681 gggaccccaa tcgtgaagca gcaaaaagga atcaacgagc tcacgcttca ttatcaaacg
    49741 ccaatctatt tctatttcat tctttctctt actttgattg gctggtttac cttgctatgt
    49801 ttatttattt atcatagata caagctttta cgtgcttagg caaaagggat aaatacatcc
    49861 gatcaaaaaa ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt
    49921 tatgttatat gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga
    49981 tcactgatga ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt
    50041 ccttcaggga cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct
    50101 attttctttt ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga
    50161 agaagaaggc aactttctct tacagtataa taaccttgag agtctactgg attattgaaa
    50221 tcacctagga aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac
    50281 tcaaaataaa agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa
    50341 atgtcctgat catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct
    50401 ttcttttgaa attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt
    50461 gcatttcctt cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac
    50521 tcttttccgt gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata
    50581 ggtacttgat tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat
    50641 tccagtaatt ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta
    50701 tctgctaatt cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt
    50761 gtcatgaatt tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa
    50821 atttttgtca caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct
    50881 caatggacca ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt
    50941 ttcacgacct ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca
    51001 aacgaaaaat aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc
    51061 tttttcacaa cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta
    51121 cctaattgct tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt
    51181 ggatcttttt cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt
    51241 cctacttctt ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta
    51301 tcgattccca tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt
    51361 ttgctaggga aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt
    51421 ggaataacag caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca
    51481 gataaaggaa tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat
    51541 tgcttactac ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct
    51601 cctgattgca gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact
    51661 gcttggactc cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct
    51721 tcagtcccta ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg
    51781 acatctttga tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct
    51841 gctgttcctg ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg
    51901 ttacgtcccg gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta
    51961 acaacaccaa aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct
    52021 ttgatagcta acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat
    52081 ggcaagatat ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat
    52141 ggcacagcag caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca
    52201 gccaacgctg ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta
    52261 cgatacatcg caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact
    52321 ttgaaacgag ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca
    52381 cctttaagat tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact
    52441 tgtgtccccg cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg
    52501 ataggaatcg tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca
    52561 aataaaaacg gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa
    52621 ttattgattc cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt
    52681 gaccataaga taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc
    52741 ggcaattttc tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg
    52801 atccctacga atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct
    52861 ttaaccatga tcttcgtagc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg
    52921 tcacttgtga caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca
    52981 gctgcaaatg ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat
    53041 aaaatatgaa gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg
    53101 acaagcggcg tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac
    53161 ccagctgccg gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac
    53221 tcaaaactaa atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac
    53281 gtattatagc gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac
    53341 cattctataa aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt
    53401 tttttaagct tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc
    53461 gtttattatg aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga
    53521 ctatttgcta ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga
    53581 ctgcaagaat ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa
    53641 gaaacttact caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat
    53701 aagacacgtg tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac
    53761 gccttgaact ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct
    53821 ttggaaccag aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca
    53881 ttcacaatca ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt
    53941 gtcaaaaaag aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca
    54001 gtcaaactcg tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa
    54061 catttttgga atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa
    54121 actattccaa acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc
    54181 gttaatggaa aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc
    54241 ttgtctgcag aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt
    54301 actagtagag atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac
    54361 cagacaaaca attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg
    54421 aagcttgccg atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat
    54481 ttgttccaac tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa
    54541 ggattcacag gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc
    54601 cctctttatc ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat
    54661 aatcaactgc cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat
    54721 cctatggtca ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag
    54781 attcaccgaa atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat
    54841 acggaatatt tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca
    54901 gaccgtgtcc atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca
    54961 aacgaatatg agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc
    55021 ttgcgttaca cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc
    55081 gaagaagaac tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa
    55141 gaattaggaa tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc
    55201 gatattccca caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga
    55261 tcttgcttca tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt
    55321 acaaaagaag aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct
    55381 tccctttcgc caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct
    55441 gtagaaatgt accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa
    55501 gacggcttgc acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct
    55561 caaatgaaaa ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa
    55621 gcttattctt tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa
    55681 caagttactt tcaagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct
    55741 tattctttag cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg
    55801 ggtattgttt gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg
    55861 gcgaaagctt ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa
    55921 aggcgtcagc cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc
    55981 ttttacaaag gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat
    56041 gatccagacg atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt
    56101 aaaagaagaa aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact
    56161 tgaaaaaatg ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa
    56221 cggaaaacct gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga
    56281 agaatgtatc gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc
    56341 tcaaccaatt ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga
    56401 tacgactttt cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag
    56461 agatctccgt cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact
    56521 acttagaagt cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca
    56581 aagaaggaaa atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag
    56641 acaaaagctt ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt
    56701 ggggtgagca tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag
    56761 gctggtcttt ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg
    56821 tcttttactt aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt
    56881 accgtttaac aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca
    56941 tctgcaatcc gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct
    57001 atgaacatca gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc
    57061 ctactggaaa ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac
    57121 tgcaagagat ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc
    57181 ctaggctttc caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta
    57241 tagcaacttc taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct
    57301 ctgtcaatgg aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc
    57361 ctgatatcgt tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga
    57421 tcagtcagac gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg
    57481 ctattaccgt aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg
    57541 tcatcaacga tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg
    57601 atgaactagg ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg
    57661 ccatcggcgt aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc
    57721 ttgagatcat ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg
    57781 ctactgctaa aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac
    57841 aagtagatgg ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat
    57901 accaaaacca catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg
    57961 tttatgtaga taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga
    58021 atggacataa gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac
    58081 gatatttcgg ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag
    58141 tactagagca tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca
    58201 cagcattggt ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt
    58261 atggctatgt cattccagag acactctccg tcattagctt caacaactcg atcttctcaa
    58321 cattaacaca cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg
    58381 ctatgcaaaa gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca
    58441 tccctcatcg cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag
    58501 ctgactcact aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat
    58561 actacttcta acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact
    58621 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
    58681 agctgaaatt cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag
    58741 ttaaacgctt ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt
    58801 caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg
    58861 caagtaatgc aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca
    58921 cggtacgaga gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt
    58981 tgataagcca acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag
    59041 atactgttct ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa
    59101 acgagaaggt cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata
    59161 tttgatcagt aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat
    59221 actcatgatc ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac
    59281 ataaccattc atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa
    59341 agaaagaacg agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt
    59401 gattgccccc cccaaataaa ataactagtg gaatcaatgt ccaaggagag atccaaaagc
    59461 tcgctttcat gttttggatc atttcattga tactagctag atcggctgaa tgactgttat
    59521 gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc tgggaaatcc gtataacgca
    59581 taccagcgat atgttcgaat agattaactc ctccaatacc cgctgccaag ttggtcgtat
    59641 ctgataaagg cgagcagtta ttccctaaaa aggcaccgga aacgactgcg cctgtagtca
    59701 ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa agcaattcca atcgtcgaaa
    59761 tcgttgtaaa tgaactgccg acagtcactc cgacaattgc acaaacaaga aatgctgtcg
    59821 gtaaaaagaa acgcacagaa acaagtttaa atccataaac cataatcgta gggatggttc
    59881 cagataaaat ccatgaagca actaagacac caatcaaaag aaaaatcagg attggaatga
    59941 tccctggagt gattcctttg atgatcccgt catggatctc atcccatgtg aactttttaa
    60001 atctgccata aaacaacaaa cacatgaaag ctgcaaggat cgggatatgt ggcgagagct
    60061 ggaatccgat gatcagtact cctaggagtg ccaacaacac gatcaataca gcgatacttt
    60121 cttttaaaga aatattttta ttcatcgtcg atctcctatt tcaaatgaaa aggccctgcc
    60181 atgaaaaaac tattcacttg gcaggacctc ctactaaatc agcgacaaaa cgtcggctga
    60241 aagacttatt tttttatttt tcttctaaac ttttttctac ctcaatcccc caagaatctt
    60301 tcttgaagtg attgatgatc aatggaataa cgaacatgac cactgtgata gctgctacta
    60361 ataatgcatc actcaatgtc gctgtaccta atgtagcaaa tacgacaacg atgatagaga
    60421 agatcagcat gcagctgact acccaagcta atccgtttcc gtttttacca attcggtaag
    60481 gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa tgaaacagca atcaaaatgt
    60541 acacgataca ataaataaca gttgtagcat ttgtaagtgt caagaataca gcacttacgt
    60601 catccattac accataaagc aaagcaaata atgaaataac gattgattgt gttagaacga
    60661 catttctaga tacgccgtat ttgttttctt tatggaatcc gaattttggt ggaaggaatc
    60721 cttcacgagc tacttggatg attgtttttg atggtccagt aacccaagct gataattgta
    60781 aaagcacacc aataaatacc atgaagctga agatatttcc gatcacttct ggtaagccta
    60841 ggatttgaca gtcaatcaag ataggctgcg tgatatttgc taattccatt tttccatctg
    60901 gtacgacatt tgctacaagc atcgcattga tgacattcaa taaaactaat ccgatcaaag
    60961 cgataaatac gccttttgta taattttttg ttgcgtcttt caaacgaggc atgtaaacag
    61021 aactcatttc gatcccaaca aaaataaatg tgataccagc taagtatttc aatgaatcaa
    61081 tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc acctagatag ccgtttgaga
    61141 ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa catgatgact actgggatat
    61201 atacccctag ccatacaccg atgttcccgc cgacttttgc catatcgaat ttcaagttca
    61261 agatcgtgat gatccagtag ataactaaaa tacaacctaa aacgaaccag tgattattgc
    61321 caagttctac attaccaaat gtgtttccta acaatggtcc taaagtagaa gcgaccatta
    61381 ccatacctgg gaacatttgc acccataata gccatgcaac gacaaatccc catttgctgc
    61441 ccagtgctgt ttttacccat aactgaggac cgccttcttg aggaagcatc gtggaaagct
    61501 caccggaaac cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt gaataaaaaa
    61561 tcaatgtcca accgactgcg gctaacgtag gaatactccg aaccgttgca cacagtgcca
    61621 ttgtcagacc gataaaggtc ccaagaccca ttttacttac ttcagattta ctcaccgttg
    61681 acactctcct tgattttttg gataaaataa cggtgtcttt gaagtagttt cagcaataaa
    61741 cgaaacaatt cagcgaatat ttgaggggat tctttgagtt tattgcttga agggtttctt
    61801 caaagacaac ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc tcttcagatc
    61861 caaacatgcg tcagaaacag ttagtattca agctgttttc taacatgatt accttgctta
    61921 ttttgcttcg cttgccaata attggtctgc gtagattttt tccaattttt cttgaagagc
    61981 tgctttgatt ttttcagcat attcttcaaa gtgttcttct ttgttcatgt atggtgtcat
    62041 tacgctggca cgcaatacag ttacttttcc ggcacggttc cattcttcat ctgagaatcc
    62101 tagttggttc acaaattgta atgggctgtt gccatagtct gggatagcaa agtcagtatg
    62161 tgaagttaag aactcattac cgtaaatgct tcctttcacg tatgaagaat aatcatatac
    62221 gtcatggttc aatttattca ttgcaactaa gtcatcgttg cctttttctt tgaatacata
    62281 gtctaccata ttgaaatctg gataagttaa tggatgaact tcgatttctt tgtctcctac
    62341 tttaaatgac aagtcattca agaagttgta gaaacgatgt gcaccttcga ttgatgcacc
    62401 cattaattta ccatatcctg tgacattcaa aggtaagaca tggtgtgctg cccaaacgct
    62461 ggctgctgtt gcccctgctt ttgaaccttc caagatataa gccccaagta atgctgggat
    62521 atctgcgcct ttttcaaata catatgtcgc aaagtaagag ataacgtctc tcatgcggat
    62581 atctttgatg acgataccac cggcagaata tggaacatag cccattttat gtgggtcaat
    62641 cgttacagat tctgcttctt cgattgcttt atatgcactg tgtacttctt ctaagatata
    62701 atctttgttt tctgtaaaga cattgtattt gtaatgaaca tcttttaaat cttcaaatgg
    62761 aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat aagcagcatc
    62821 tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg caacgatttt
    62881 atcgataccg tcgattgctc cttcttctgt tgatcctaca acaccgacta cacctaggat
    62941 tggtgttttt tcagctgcta gaccacgaac gattttttca agttcgttga tgtccatgcg
    63001 atagttgtgg tcaactggca ctggaatcac ttggtctaaa ccgataccga tgatgtcggc
    63061 agcttttagc catgaatagt gtttagtttg tggaaccaac cattttccta atttttctaa
    63121 atgttttccg ctacgtgctg agtgagcttt gatttcgtcg attttttctg ggacgctgtc
    63181 taacagattc atgatttctt ctgttgacaa gttcattaat tcccaatcgc tttttccagc
    63241 aactagttca ggtgtcactt ctttcattgc aagcggcaat gatttgatgt tgcgggcata
    63301 ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg atgtggcccc agccatcttt
    63361 atagctcatt aattttgcaa attccattcc tacttcttct tccatttggc ttgttgcagg
    63421 agaagattca taggcaacgt tattaccatt ccatagcata gcaaaattgt acgctagtaa
    63481 agatggcatc aatgtttcag agttcatatg accccagtaa cgtccggcat tatgccaagg
    63541 aacggaatgg gtacgcatcc gcgctgaaat ttctgataga acatcctttg ttttattcac
    63601 agtgtgttca aaactagcac tgcttttttc ttctggcgta ataataggca tgtcttgagg
    63661 catgtagttt tgacgccagc ctaaatgttc atccaccaat tcattcaaca aagctttata
    63721 gatttggccg ttctcagctt tgtccccaat gaatagggca tttaggttta gatctttcga
    63781 caatgattca ctcatagtga aaccctccta aaaaaatatt taaattttgt cgagccctac
    63841 attacacctg ttcttctatt ctgtaaacta agttttggcc tgatttttaa tcatatttta
    63901 atatgtatca attatccgaa ttgttcacac ttttgaaata attaaaaaaa acgagggaat
    63961 ttacaaatgt aaactccctc gcaaagattg tattaacgac aaccagcgtt ctattttagg
    64021 atgggtttca aaattggaat gttgtaatag tatttccctt tttcaaggta aaaacttact
    64081 aaattctaat caattacttt ggccaaaaag taatttttct taccttttcg aataaccaca
    64141 aacttaccat cgaattcatc ggatggattt atgacaaaat ttaaatcggt aacgcggtca
    64201 ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt agatggttca
    64261 atttttgtgg aaacaagcag ttccacgata ttttcaggtg tgcttgaaat ttcaacgttc
    64321 ggcattttac cgaatccttg cgcgatttct tccgcattta gttctttaat atttccagag
    64381 aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt cttcactatg cacaaatctt
    64441 gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct caggttctgt ctctactttt
    64501 ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa agaatttcaa gtatttcact
    64561 acatcgcgat catcttgatt caaccagaat tggtagaact caaatggtga agttttcttc
    64621 ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc agccttcagc
    64681 atcaatggga tcgttaaacc gaacgcttct gcttctggtc cttctaattt tcggattagg
    64741 tctaatccag ctgtgatatt tccccattgg tctgctccac cgatttgcaa ttgcacatcg
    64801 tatgttttat gcagatgcaa aaagtcgata gattgcagga tttggtaagt aaactctgta
    64861 aatgagattc ctacttccaa acggctggca acgatatctt ttgctaacat cgtgttgatg
    64921 ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag agatttttga caaccagtcg
    64981 tagttgttca caaatgtgat atttgcatct ttaccaaaca attttctcat ttgattggaa
    65041 agtgcgtcca cgttatgttg cacttgttcc atcgtttgca acacacgttc tgttttgcgg
    65101 ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca gaatatatgg atgatggcct
    65161 gccaattgga atcttttcat catcataaat ggaatcaaat gtccgatatg catcgaatca
    65221 ccagttggat caacgccgca ataaagtgaa atgctctttt cttcaacgag ctcgcgaagt
    65281 ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag ttaattcatc aataatattc
    65341 attttactga cctccgtgta gtttttttat gaaagcagga acctgctagc atgtgtaatt
    65401 aattatttct tcaggatagg ataggaaata aaaaaagtcc cttggcatat aagccaaggg
    65461 acgaaatctc gcggtaccac ccaatttgta tgtctagata tccaacatac ctctcgaaca
    65521 tttaacgctg tcgcgtggca atcactgcca aagactcctg aaatgtaatt cgctttaaag
    65581 catgtactgg tttgcagcga ccaccagctt tctgaaacat tggctttaac aactacttcg
    65641 tttcgatctt cgtcttgtta ttattaatat gtgtctatca tagagcgcaa ctttttcttt
    65701 gtcaacagaa aaaggatatt ttttttaaat tcctcatttt ttcttttttg aatccgctct
    65761 aattccgaac attttatttt caattattgt taaaagaaat aactttttct gtattttttc
    65821 tgcaatttat cgagaagtca ctttactttg cctcttattc tttcttcgtt acaataagga
    65881 atgggacaag taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa aagcaaaaaa
    65941 ttattttagg agccacttct gctttaacta tcgttatttt aattctaagt ttgttttttg
    66001 tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata acagaaaaag
    66061 caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat ctggctcaaa
    66121 gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac ggatctactg
    66181 aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc gactggtctg
    66241 atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg caaaaagcct
    66301 tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa gaattcaacc
    66361 gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat gatttgatcc
    66421 aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa ggaaaaaaac
    66481 cattggttct ttcacaagat gatgtcagct attatgaata tatggataat tccggttttc
    66541 ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa gagaaagata
    66601 aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc aagaaacatc
    66661 ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac aacggagttt
    66721 taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa aaagagatta
    66781 aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt gcttcccata
    66841 catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa gacaatgaat
    66901 tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt tatccatttg
    66961 gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt gcttacttga
    67021 agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct tggggacaat
    67081 taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt tttcaatctg
    67141 atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat gtctatgaca
    67201 aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag ccgaactgac
    67261 attttatcgt cagtttcggc ttttttatgc tttatttctt cttcggtgcg attcccttga
    67321 tcaggacttc tgtccattct gctgtatatg gaagcagttt tgcgtatatc tccggcttag
    67381 aaattccttc taccattgct tgaaaataga ggatcaagaa ctcatccgaa tatttcaaat
    67441 ccacttttcc ttctttccgc cctcgatgga aaagatcaag catgatactg aaactttctt
    67501 gcgtatacgc atccatcgcg tcagaaaatt cttttttttc tttttttgta tagtatgtca
    67561 tcaaatcgat ataaaattgt ttgttcactc gattgagata acggattttg ttttcactca
    67621 tggcaatcag tgtttcttca aatggaagat tttccgcaat caacgctttc gcttcttgcc
    67681 ctaaatcatt cataaatgca aaaaatactt cataaatcag cccttccttg ctgccaaaat
    67741 atttaaaaat cgtcgcttta cttacctgag cgttctcagc taccttatcc attgtcactc
    67801 cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc tctttctttt
    67861 tctcttgtgc tctcttttca aaaccgttca tttgtagagc ctcccgtaat tttaaacttt
    67921 tataatatat atagttcaaa tttattctga ggtttattat aactatctat tgagaaagta
    67981 tcaagctagc aattccttgt ttatgaattc attgacagaa atttataaaa agaatattat
    68041 gtactaaaat aataaaaaaa gttttataaa aaggtataaa acctttttat taaactattt
    68101 aatcttgttt agtatatatt tttagaaaaa ggagtggggt ttttatggca gaaatcgtca
    68161 aagtcacaca tttgaaaaag agctttggtc gtttcgaagc tttaaaagat gtctctttca
    68221 ccgtacattc aggtgaagtc gttggattta tcggaccaaa tggttctgga aaatccacga
    68281 cgattcggac acttttaggg attataaaaa aagacggcgg aacggcaacg atttttgggc
    68341 aagatgtatg ggaagatagt ttatctatcc atgaacgtct ttcttatgtt ccaggcgata
    68401 tctctttgtg gggaaatttg actggaggag cgatcatcga tctcttcatg aagcttcatg
    68461 gaaaagggag tgctcaaaaa aaagatgaac tgattcaacg tttctctctt gatcctaaga
    68521 aaaaagcaaa aaactattca aaaggaaatc gccaaaaagt gggcttgatt gctgctttgg
    68581 ctgttgattc ggatctctat ttatttgatg agcctacttc tggtttagat cctttgatgg
    68641 aaacgatttt ccaagaagaa gttgaaaaat tgaaagaaca gggcaaatcc atcctacttt
    68701 cttctcatat tctcagtgaa gtcgagcgac tagcggataa agttgtgatc atacgcgaag
    68761 gaacactcga tgaagaagga acactcgatg aattgcgtca tttgactcgt tcgaccatca
    68821 ctatagaaac agaagcagag aatagtcact tagcacaatt gaacggtgtc catgactttg
    68881 tgcaaaaagg tcacctcgct caatttgctg ctgataacga agctttcgat gtgattttag
    68941 ctgaagcgtc caaattaggt gtgaaaaaat ttgaatctat tccaccgact cttgaggatt
    69001 tattcatgag acattatgaa ggatgaggag gaagcgaaat gactgataaa ttttcacgct
    69061 ggtccaccct tttttctcaa aaccttagcc gtgactggaa aaagatcctg atatggctag
    69121 tcggtgtcac tgctttttct ggtggttttg tccctgcgtt tgaagaattg acttccggac
    69181 aaggattagc tggattatac gaaaccttgc aaaacccggc aatgattgca atggtcggac
    69241 caactccagc ttcgaatgct gctgagtata ctttaggagc gatgtatgca aatgaaatgt
    69301 tgcttttttg cagtttgttt gctgctgtga tctctgtttt acacgtggtg agccaaacga
    69361 gaaaaaaaga agaacttggt ctgattgaac ttattcgttc ctttcgagtc ggacgtcaag
    69421 caaactcttt agctgtcatc gctgaaagta ttctgatcaa tttgctttta gcaatatcga
    69481 tcagttgtac gatgattttt ttccaagcgg attcgatcac tgcagaaggt gcttggcttt
    69541 ttgggctatc tgtaggtagt atggggattt tggggagtgc cgttgcactg tttgctgcac
    69601 aactgatgcc aagttcagcc agttcgaatg gtctgtcttt agcgttttta ggtggacttt
    69661 accttctacg agcaataaca gatgtagctg attcctccct ttcgatgtgg aatcctttag
    69721 gctggtctta tttgacctat ccatttacaa ctaacaactg gattcctttg ctttatttgt
    69781 ttttgttcag tctaatcttg ataatcagtt catttgtttt agaggaacat cgagatttga
    69841 acaccggcta tatccccgaa cgtgaaggaa aaccgtatgc taaacgttct cttttatcct
    69901 tgcgtggttt attattctat ctaaataaag ggatgacaat cagttggctt atcacttttg
    69961 gtttgatggg ggcggcatac ggctctatct acgggaatat gcaacagttt ttagaaagta
    70021 acgacttaat gaaacagatg tttatccaat ctggcgtctc aattgaaaaa aactttacaa
    70081 gcacgatcat ggtcgtcttg atttgtctag ctgctatttt accaattgct agcatcaatc
    70141 gtttatttac agaagaaacc cacggacgta tgaatcagct gttcgcaaca aaaatttcta
    70201 ggaaccaact ttattggacc aatatgttac tagctattct atttgctata ttggcaatcc
    70261 tgattagttc tggtgcacta gggctttctg ctttggcttc gatgaaggat gcgaaagaca
    70321 tggatctatt gatgtttctt aaagccgggt tcaatttcct gcctgccgtt ttagtgttta
    70381 tcggaattag tggctttcta ttcggatggc ttcctcgaat cggtaaactt gcttatatct
    70441 acttagctta ctcctttgca ctcaattact ttggcggaat tttggattta ccagaatggt
    70501 tttccaaaac agctgtccct agctgggttc cacggatgcc aaacgattct tttgatcttt
    70561 ccgtgttcct tacgttatca tgtatcagcc ttgcactgtt cgtccttggt tctattggtt
    70621 ataggcgaag agacttacta gaaggataat aaaaaagcat gtacaatttc atgctagcct
    70681 ccaaatctgg gtcaaaaatc caacatttgg aggtcatttt tttatttaca agtcttctaa
    70741 ctgctcttta tcaatgatcc agacttctcg attctttctt tttacataac cattctcctc
    70801 taataattta aattttctag ataaagtttc gggggtcgta ccgataaatg cagcaagttc
    70861 tttcattttc attggcagga aaaagtgatc cttccctgtc gctttagcta agtcaagcag
    70921 atagacagcg actcgttctt ctactttttc catcatcaaa aaagttgtct gctgctgtac
    70981 ttttgttaat ttctcaacgt tgattgctaa aagtttcaga ctaagggcgg gataatgcag
    71041 taagagttcc gagaaatctt tttgtctcaa tatgcaaatt tccgtttttt ctagtgcttc
    71101 gccaaataaa gactcatttg gaacgcctag caactggctc tctccctcat atccgcccgg
    71161 ctctattact cgtaaaagct gttcctttcc gctagcagat aattgataca ctttcatact
    71221 gccggaggcc acgatcacta attgaggttc tccattcggc gcaagaatct gttctccttt
    71281 ttccacatag tgatgtttga caagtgcgtg tactttttct tgatcttgct gtactaagtg
    71341 attgaacaaa ggaacaaatg aaacacataa atgatcatga ctcataatct ctcctccttc
    71401 taaacagcta tttctaataa agaaacataa cttcccttat tccaactgat aattttcttt
    71461 tatcatagca taaacgtcaa gcgaaccaga taccagcaaa tagagtggat ttctttttgt
    71521 cccttgcttt attttccttc ttttgagacc ttaagtaatt gattcctatc aattttttaa
    71581 atatttactt tttgaataat caaaaacaaa ccttcactcg caagccgaga gtaaggtttg
    71641 tttttcgcat tgcttatttc gtgttgtcta ttgaaatttt tccgcaccta gcttttgata
    71701 tagtggtgct agttcttgta aggtttcagc ggttatttgc tcaacgtgtt cgatttcttg
    71761 cggatagaag aatagtttac ccatttgaaa ggttcggtct ctcatcgctt ttttcaactc
    71821 ttcttgtttc tttgtatcgg acaaatagtc agacaaagct aagtggtcgt caagttcatg
    71881 ttctggttgc ggccatatgt aataatcctt gatatcgact gttttgctcc actctttcag
    71941 tatcgcaagc aactggttat acgctgcaat gctaccatat cgttcttcgc ttttataatg
    72001 ttggtacatc aaatagattt ggtattgttt ttgattcaac aaaactgcta gacaggcatt
    72061 ctcgtcttga cgagattcgc ttcggtacgc gctccaaaaa tgactgcgta aattccagcc
    72121 attcgtccag ctttcgattt taggtttgct catgccgtac atatcaggca aaagtgcagc
    72181 tgtctggagc tgaatctctt tccatttttg ccagtgttct ttatattctt tcttgatttg
    72241 ttcaatctta tctgttggat ggctctcttt gagctgtttg aatgcgtaga ttggtcgatc
    72301 aaacaactga taatctgatt ttccaagttc cattgtttcg cctctttccg ttatcttttg
    72361 tattctacct gaattttgcg atatggacaa atagaggttg tgaaaaagct gttctgcatc
    72421 aagtcataag aacagccaaa caaaaaactg cgatgagcat caccgcagtt ttattgaact
    72481 taaggttgat tgatcacatc taaaagcgtt gaaaataaaa tggcactaga agcagctcct
    72541 gggtcgatat gtccgattgc acgctctcct agataggaag ctcgtccttt tttagctacc
    72601 aagactttgg tgtgttcttt tgcctggtca atgatttctt ttgttaaatt ccctgccttc
    72661 aatgcctctg tgactggcag ccaaacatct aacatcgttt tatcgtcagc ttctgccttt
    72721 cctcggcttt gaatacccgc tgttccctgc tcgatcactt cgcctaattt ttcttgagaa
    72781 tcaatagaat cgacctcttt tgtcgctttc atcatgttca tgaatgcaga tccgtataac
    72841 ggaccagaag ccccaccgac tttcgatatc atcgccatag agaatacttt gaatgtatcg
    72901 ctgattgttt caggatgatc ggcttgaaaa gcttcttcat aagctgaaac ccctcttccc
    72961 atgttatttc catggtctcc atcgccaatc ggggtatcta gatcactgag atattgtttg
    73021 ttctgattga ttgcctctgc aaattgcgtg agccattctt ggatttcttg aacggttagt
    73081 ttcatattat caccctttta tcttgagatt ttttaccatg aaatcgtctg tacattgctt
    73141 tctaaagaat ctttccattg tgtctccgtc aagtcgatca gtgttagaga aagaccttgc
    73201 atatctagtg aggtcataaa attaccgact tttcggaaaa ctacattgac gcctctttct
    73261 tccaacagag ctaaaacatc attcatgaac acgaactgtt ccatcaaagg cgtcccgccc
    73321 attccgttga ctagtacacc tgcttctttt ggtaaattcg agtaatcatc taaaatctta
    73381 gtaacaagtt cctttgccag tactttagaa ggctgcatct tttctcgacg atagcctggc
    73441 tcaccgtgga taccaacacc atactcgatc tcatcttctc ctagttcgaa tcctggtttc
    73501 cctacttctg gtacagttgc tgctcgaagc gctagaccga tcgtcttagt tgcctgtacg
    73561 accttttctc ccagttcttt caactcagct agggaagcac cgtttcttgc tgcatcgccc
    73621 acgattttgt gtacaaggac agtacctgca actccgcgtt ttccagcagt atacgtacta
    73681 ttttcaacgg cgatatcatc atctacgatc acactttcta cttcaatatc gtccatagca
    73741 gctaattcct ttgccatatc gaagttcaat gcatcccctg tataattctt aacgatcagc
    73801 aaaactcctt ttccactatc ggcttcctta atagccgtct gaatctgatc tggagttgga
    73861 gaagtaaaca catcaccaag caccgctgca cttaacatcc cttcccctac aaacccggca
    73921 tgggatggtt catgtccgct tcctccacca gaaaccaaac caacttgttc cgctttctga
    73981 ttcttggcta caacacggga agattctaca cgatgaacaa gttctggata gctttttact
    74041 aatccttcca gcatttcttc gacaatattg cttggatcat tcatgatttt tttcaattta
    74101 ttcccatcct ttctttccgg cattttgtgc ttctgttaag atttgctgag tatcatctgt
    74161 tataccggca gtgatggctg ctgcaaaagc tccttccact aaaggcgcat ccaccagatg
    74221 ataacgttgc tgctgctctt cttccaacat gtcttttgcc agttccgagt tcaataccgc
    74281 gctaccgata tccgcaaaaa caaaataact agctgcatcg cttgattcat tgactgcttc
    74341 tacgattttc atcgggtctg atccgatctc gccatttatt ccgccgagag aaaaaatttg
    74401 cacctcttcg gatttttgca tctgctcaat catttcttta ataccatctg ttactttttg
    74461 actatgagaa ataagcaaaa tacttttttt cacaagaatc ttcctttctt ttacatagtt
    74521 gctaacgatt ggtttcgctt tcattaatga acaattcaag tatataataa atttctaatt
    74581 ttgggaaaat atgtgtagtg cttcttgaag tttgttatac tttaattaaa agagaggagt
    74641 gatcgaatga ttacaacaac aacgaacagt gtagagggaa aagccgtagt agcttataaa
    74701 gggatcgttt ttggtgaggt cattactggg atccatgcat ttaaagactt ggaggcgggg
    74761 ttgcgaaata tctttggcgg ccgctcaaaa agctatgaaa acgaactaat gggtgcaaga
    74821 gaagaagcac ttgccgaaat ggccgaacgt gcaaaagcat taggagctga tgcgatcatt
    74881 ggcgtaaaaa tggactatga agtattaggc agcgacaatg gcatgctgat ggtcacatgt
    74941 agtggaacag ctgtctccac gagataagta ggggacatct gttttaataa ctaaagagag
    75001 atgggacatg tagtggcact caagaattag atttttcagt ctaacttcga ggtactctac
    75061 cttcccatcc tctctttttt atttcttttt gcggatgatt ccgtaagctg ctgcgactaa
    75121 cacacccgcc cctgctaagg aataactgac agaagctgtt ttttcatttg tttttggcaa
    75181 agtctgactt gttgatttgg atggctctgt cgtttgctga actgtttctg tcgtgcttcc
    75241 agaggtcact gatttttctc ctgccgaatg caaaatatct tttattcgtt ggaaactagc
    75301 tgctaagtca gctttttttg cttcaaaatc attagcaact gtttgtgctt cgctgatctt
    75361 tccgtggatt gcttgactgg ccgctgtgat atccgcttga gtttgactta gctttccaga
    75421 agctgtttga gcaacctgat ttaattgctg attgaattct tttgcttgtt ggatcttccc
    75481 gtcaagatct gtttggacag cttccgcttt tgctttcgct tcggcaagct ttttctcaag
    75541 ttgtgcacgg tcatttccca ctttattttt aagatcagct aaatcttgat cgattgctgc
    75601 ttggtcttct ttcaatttgt tttttgcagc ttctaatgct tcttcgagtt cttttctttt
    75661 attttccaag tcttgcaaat gatcgatttg gtcagaagag gtagaactat ttattgatgt
    75721 atcttctgaa gatgaaatcg tttctcctga attatctgtc ggtacattgg aagtcatttc
    75781 tgtttccgag gcaccgtttt cagcaccaga agagcttatg gttgttgaag gttctgtttg
    75841 agcaggttca gaagtttccg caaaagctgt ctgtcccaat aagaaaatac tggaaacagc
    75901 aattggccag attaacggct tcattctttt tttcatcact tttcccatcc ttactacttt
    75961 ttaactaatt tcttattata agcaaaaatg tttggaagaa ccataaacac aacaggctgt
    76021 ttgtctaaaa aacggtgcat cttttatttt tttgtacgtt ccgggctttt ccatgtcccg
    76081 cttccataac ctaaaatcgt tttgactgct ggaatgaaag tcgtcacgat cgttatcgcg
    76141 ttcactatcc agaacaaaag catatatagt ggtgcgaaaa gaaggtattt caattttctt
    76201 cgtcgatcat ctacaatcag cgaagctagc aactggaaaa atcctgcgac catttcaaag
    76261 cagatgaaca caaaactgaa tgtaaaagta atataaatct gttcgtagtt tccttgatat
    76321 acataatgcc cgattaatcc agcaaaaaga actacggaaa tacaaaagaa tattgaccag
    76381 acaatactca atgtttgatc aatgaagatg atcgttcttc caatatgttt aaaaggatgg
    76441 agcatcactt ttttgaaatt cgtcagccag acttctgttc caccttttgc ccaacgtttt
    76501 ctgtgtcgat atagcatctt caacgtcaca ggtacttcca tgaaaaagat aatctcagga
    76561 gcaaaaacag ataaccagtc atttaactga tgatcccaag caatactgat gtcttctgtc
    76621 gctcgatctt gtctgaatcc gcccacatcg atcaaggctt cttttcgata catcgtattt
    76681 gctccactgt acgcataaag accaccgaaa acagctgact gtgttcgttt aatgatgccc
    76741 acgatacttg agaattcaac cgtttgagat tttgcaatca atttggtacg attttggaca
    76801 tccatatttg cagtgaccgc agcaatatgt cttgcaccag ggcgaatgaa ataattcaca
    76861 tacctaatca gtgcatctgg ctccggtacc gtgtctgcat cattgcttaa aatcaatttc
    76921 ccttttgcaa aggcaagtcc gatattaaac gcgtgggctt ttcctttatt tttttcgatc
    76981 cgaaccacac gaagattagc gtattttttc atcagtctag ctaatatttc tggtgtttgg
    77041 tccgttgaac catcgtctgt cactaatacc tcatagttgt gataattcaa tttcgtcatc
    77101 aaatactcga ttgtatattc gatcacaatt tcttcattat gggctggcac catgatcgtg
    77161 atgaacggtt cgacactcaa gggaacatct acccattctt tttgcttatg tttgtataaa
    77221 aacacataac accagacacc aataaaccat acaaatccgc ctaaaatcgg ataagaaacg
    77281 agaaaaaaat acataataga caaacaagta tctaagaata aattacctgt atgggtgatt
    77341 gagatcattt cagttccacc ccattttctt tatacaaatc tgcaatgaat gtcgtatcta
    77401 gatttttctc ttcggagaca gaataaaaac ggaccgtttc tctctcttct tttgttccaa
    77461 aacgttccgt atacacttct tcaagtaatt cttgtctttt tttcaatttt tcatcttcat
    77521 attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta gtcaatacaa
    77581 atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca
    77641 ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta
    77701 atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta
    77761 acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa
    77821 ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac
    77881 gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc
    77941 taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca
    78001 gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt
    78061 tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt
    78121 tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa
    78181 tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg
    78241 tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct
    78301 cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc
    78361 cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa
    78421 gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca
    78481 gatggtagtc gtcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg
    78541 cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac
    78601 ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta
    78661 catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag
    78721 tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca
    78781 actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt
    78841 tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt
    78901 taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag
    78961 gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg
    79021 cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg
    79081 tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat
    79141 ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt
    79201 ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta
    79261 tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt
    79321 cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa
    79381 tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct
    79441 gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt
    79501 tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt
    79561 cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg
    79621 tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa
    79681 ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa
    79741 aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga
    79801 aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga
    79861 cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt
    79921 ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag
    79981 tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca
    80041 acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat
    80101 caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga
    80161 ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga
    80221 gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg
    80281 acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc
    80341 aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt
    80401 tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc
    80461 ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat ttttcctttt
    80521 gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc
    80581 attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac
    80641 gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa
    80701 atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca
    80761 gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca
    80821 tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt
    80881 tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata
    80941 ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga
    81001 ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg
    81061 atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg
    81121 atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga
    81181 acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat
    81241 cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga
    81301 tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt
    81361 ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc
    81421 aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc
    81481 atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc
    81541 cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt
    81601 tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct
    81661 gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt
    81721 tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa
    81781 tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat
    81841 cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc
    81901 atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac
    81961 tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg
    82021 acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg
    82081 aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag
    82141 taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga
    82201 ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt
    82261 atattaaaca ggagctttat taatatgtca accagaatga ctcttcattt ttttcactac
    82321 tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga
    82381 atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca
    82441 aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca
    82501 attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct
    82561 gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca
    82621 agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata
    82681 ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt
    82741 ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact
    82801 ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta
    82861 tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag
    82921 gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag
    82981 tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct
    83041 acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga
    83101 acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg
    83161 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt
    83221 agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag
    83281 gtcagctgat tgaacaccgt ttaaggaaga gattgtgaaa aagtcgctta gtttcgagta
    83341 ttaagaaaaa ttttgtaaaa atggatttct acatttttgc ataaatttgg cttaataccg
    83401 agaaactggc ttttgaatac cgtttaagga agaggttgta aaatcagcgg tttgacctga
    83461 gtattagaga aacaaacgga aaaatagctg ctcatatttt gccgtttttt gagctaatac
    83521 cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca tttaactccg
    83581 aatcacaaga aataatttca aaaacaactt ctcctgtttt tggttcggtt ggcttattat
    83641 cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata ggtcacaccc
    83701 cttttttatt ttctttgaaa catctttcgg tactcgacag catacaagac aaaagattgt
    83761 aaaagaccgt aagtgatgac ggcaaaaatg actctcaccc acagcgttgg taattctggg
    83821 aaaagcagca aaaggatcat gccaacatag atgacagcag tcgctacttt gccataccac
    83881 ttcgctccac ttaatttgat atttttagat agcccgatta atccagctac aaacataaag
    83941 ccttctttta tcaagaatag accaaacaac agccaaagcc acggtcgata ccatgccata
    84001 gacaaaatca gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac cttccctaag
    84061 tctgttacct gattatatct ccgagcaatg accccgtcta gataatcaga caaacctgag
    84121 acaacaatga ccgctcctgc tatataaaat gactgtctca gcacaaaata aagataaatc
    84181 ggcagcaaaa aaatacggaa tatcgataaa agattcggaa tcgtttgtat ttctttacgc
    84241 cagtctgtgc ttaatttcat tttatcgccc tacctcctca gcactctttt ctttttattt
    84301 tagcttacac ggggaataaa accaatgatt tcactgtttt tatttttctt tacagacact
    84361 gatattatga gctaatttga gataatcact tgctgttttc atcgtttact cttctttctt
    84421 cctgtcgttt gcgcatttct tttactagta ctttatgagc tttttttgat cctcgctcta
    84481 ggtcacgctg ccgtttacgt gttttataat cgataaacag actgtctaaa tcgtaacctg
    84541 tcgggtacag atcttctgct gaacgcacca gacgaacacg ctgacgaggc acgtggatta
    84601 tttttttgtc taaatataca tcaatcgtat cttcacctat atcttcataa actaacgctt
    84661 ctttttggta ttcattgaca aatatccgat caccttttgc aaagagctga cgttcttttt
    84721 tattctcctt cctatttgta actgctgtct taggaagaaa ttcttttttc tgtgtccgat
    84781 aggctttttc cgccaagtaa cgctctgctt gatggatcaa tttcatcgac atatccattt
    84841 tatgagcaat ccacaaagct tggctttctc ctgtttcttc caacccgcaa ctgatattta
    84901 ggacgaagtg cttcccgatc gaaagccata gcagccggca caaaatcttc atgatctccg
    84961 gcgaatttct tgatttctcc ataatgggtc gtggcaatga tcaatgcccc ttgctcatac
    85021 atagattcca tgattgcgat cgctagtgca gccccttcat ttggctctgt tccactgcca
    85081 atctcatcta aaagtaccaa cgtattcctt tttatctttt tcaaaatagc agcgatgttt
    85141 tgcatatgac cagaaaatgt actcaacgca ttttctaaat tttggtggtc accgacatcg
    85201 acgaaaatct catcaaagac agcaagctct gtcccttctt ttgccggaac ttgcataccg
    85261 aacatcgcca tcaaagtcaa taaaccgact gtcttcaaca cgactgtttt gcctccggca
    85321 tttgctccgg tgataatcaa gcctcgatag tctttaccga tttcaacatc caatggtacc
    85381 gcatgctcag gtaaaaatgg atgtctgcct tgcttgataa tgatatgttc cgatttattg
    85441 attctaggtg tgcttccatt gatttctcgg ctaaattttg cccgagcaaa gatgatatct
    85501 aacaccgtga tcgtttcgat caataaatcg atcacttctt cgttttctgc gatagcacca
    85561 gtcaatgccg ccaggacttg atattcttca ctgatttctt cagcttttaa caactgatag
    85621 tgttcattca atttagaaat cacagttggt tcaataaaaa ccgtcgttct tttatttgat
    85681 tcatcgataa tcgttccatc tattttattt ttatagctgg ctttgatagg aatcgtatag
    85741 tattcgcctt tttgaacaat cattgcttct tggatcattt ctttgttttt cggatgacgc
    85801 aagaatttca gtaattttga ctggatttct ttttcgatta gttgtaattg tttgcgtact
    85861 ttacgcaaat tacgtgacgc gtcgtcactg actttattat tttttatttt ctggtcaatc
    85921 aactcttcta catttatcaa atcaggaaga tgtttgctat aagcaaacaa caagggtgtc
    85981 tggtattgat tcttatcaaa aaactttgtg atcatccgac tgcttctaag aaaatccgca
    86041 tattcgatca aatcggttgg ctgcagaatc aacccttttt tgacttgttc tgtcagtgca
    86101 tcgatcctcg gcaaccccat aaatggaaca tgctgactgc tttctaagat caagcgtgct
    86161 tctcttgttt cttcttgacg atccaagaca gtttgtaggt ttgtcgagac cgtcatatcg
    86221 ctgattcgtt ttttgctata gtttccaatt gctcttgctt ggatttcctt tttgatccga
    86281 tcaaattgta attgactaat catttcgttg tccatattct ctccttttct tgaagagata
    86341 cctgtcccaa aataaataag ggcctagaga taaataaaag gatagaacac agcttaaaaa
    86401 agtcatgtcc tatccttatt agaatacaaa gatattgttt tcctgacagg taccgtttca
    86461 taagcacacc aaataaactt tgtttattgg ctattcgact tactgattag ataccagcta
    86521 cactcacaaa acatacccca ttcttctagg tcttatttat tttccactta actatagcaa
    86581 gacacatttt ggttgtcaaa ctttagtatg ttttatagat ttacaccatt aatgaataga
    86641 cacctaccgc gataattgct ccgatcattg gacctgcgat tggtacccat gaatatgccc
    86701 aatctgaagt tcctttatta gctattggca ataccgcatg tgctaatcga ggaccaaagt
    86761 cccgagcagg gttgattgca tatcctgttg gccctcctaa tgaaagaccg atggctaaga
    86821 tcaatccacc tacagcgaat acgttcgttc caggtgcaaa ttcgttttgt ccaaaagcta
    86881 gcaacccaag aactaataca aatgtaccaa ttaattctgt gatcacattt gctggataat
    86941 tccgtatagc aggacctgtt gcaaacgttc ctaaaatcgc gctttcatct ttcgttgcat
    87001 tccaatgtgg caaataagaa agccaaacaa gaatggcccc tgcaaaagct cctaaaactt
    87061 gcgcaatgat gaaaggaacg acaagattcc aactaattgc ccctgtcatc gccatagcta
    87121 gagagactgc aggattcaaa tgagctgggc ccatgaatcc agaaatatag acagccattg
    87181 ttacagcgag tccccaacca aatgcgatga caatccagcc agaagcctgt gctttacttt
    87241 tgtttaaatt cacagccgca cacacaccat ctccaagcaa aactaggatc agtgtcccaa
    87301 taaactcccc catgatttga gtcatatcac tgttcattta tttctccccc tttagttccc
    87361 gcagatctga ttctgcaatc aatgcttcta aacgtttagt ctgttgcgcc ttctcctctt
    87421 ctgtccattc aaaatatcca gcaattgcat cgatcacagg ttgcttgatc tcatcaagct
    87481 gatctcgttc aaacaacaga tgatttgtac gacgaatgag ataatcgcct ggggccaaaa
    87541 ccatttcttc ttctaaacca taacgtaacc gagctgactc agcgagactc aaaccaggat
    87601 aaggtgccat ttcttttgct aattcaaaga tacgtcgggc attagtcccg taaaaatcag
    87661 cgatataagt agcatcttct tcctctaaac cggctgctac tccttctttt gctaattctg
    87721 tcactgtttc ttctaattta gttggatcga agtttccacc tgaaatctga tattttttag
    87781 agtcgatttc tttcgtctct attccgtatt cttcttttaa taattggcgt atcaatcgca
    87841 aagctccttc tgccatctta cggtaatcgg tgattttccc accagataaa gtcagcaaac
    87901 catctggttc tctttctaaa gaactacctc tggaaatcgt agaaggtgct ttttcatcac
    87961 ggctgttttc caaatggttc aacacgtctt ctacttcagc acgagaaact ttgttttcct
    88021 tatattcact tactgtatcg acaactttat tgaagctctt gtctgaaatc gatccattat
    88081 ctccaccatt gtaatcagaa ccagaattac caatcaacag tggacgaagt cctgcccagc
    88141 ttgcttcgat atcattcaat gtgatatttg cttctggata gcgatgattg atcacatcca
    88201 atagataatc tacatcttct tgtgtgactt tcggatccgt aaaatctcct tggtaatccg
    88261 tatctgtcgt accaaagtaa gtcttgtttt ctcttggaat agcaaaaacc atccgcccat
    88321 cttgctttcc tgtatcgaag tatgtgggtt gcggtacagg cagttttttc gcatctacaa
    88381 ctaaatggat ccctttagtt ggacgcattt taggagagac tggacgcgta aaattcaagt
    88441 ttcgtacttt atctacccaa ggaccactcg tattgatcac taattttgct ttgatttcga
    88501 tcacttcatc tgtcagcagg tcacgagctt tgacgccaac gatttgatcg ccatcatata
    88561 aaaagccaac cgctttcatt ttacttacta gataagcccc atcttcggca gcctttttga
    88621 tattatcaat cactaaacgg gcatcattgt tgcggaaatc tagatagaca cccgcgcctt
    88681 ttagcccttc ttttttcaaa aacggttctc tttccaatac ttcttctggt gtcagcgtat
    88741 aattctcata tttggttcct gtcacatttg ccagtttgtc ataaaggtcc attgctactt
    88801 tgaccgagaa catattgaag gttgttgctc cttcgccctc atagattggc aaaagcattg
    88861 gatctggttt tgggatgtgt ggggcaatac tttgtacgac tgcacgttca ccaactgtgt
    88921 cagctactac ttccacatca aatgtcttca gataacgaat accgccatga acaagtttgg
    88981 tcgagcgaga ggaagtccct tctgcaaaat cctgcatttc gatcaatcct gttttgattc
    89041 ctgatgctgc tgactggact gccacaccgg caccagtgat tccgccaccg atgatcaaca
    89101 gatccaattc ttcttgctgc attttttgaa tgctatcttg tcttgtcttg tttgaaaaca
    89161 tatggatttc ctccttattc aaatgcttgg gtagctttta ctgctttttt ccaaccgttg
    89221 taaagttttt ctcggcgctc ttcctccatt tgaacttcaa atagcttgcc agcttcataa
    89281 aattcgcgga tctcgtctgt atctttccaa taaccgacag ctaatcctgc taaaaatgct
    89341 gcccctagcg ccgttgtttc caagttttct gctcgttgga tcggtacatt caagatatct
    89401 gtttggaatt gcatcagata ttcattgttt gctgcaccgc catctacttt caatacagga
    89461 atcttgattc cagtgtcatc ttgcatcgta tcgatgatat ctctcacttg ataagcaatc
    89521 gactgtaaag tagctttaat gatatcttct tttgtcgtac cacgtgtcaa gccaaacata
    89581 gcccctcgtg ctgcctgatc ccaataagga gcacctagcc caacgaatgc aggaacaaca
    89641 taaacctcat cttcgcttgt agagcgtttc gctgcgtctt ctgaatcgct agctttttgc
    89701 aacatttgaa gtccatctct cagccattga atcgaagaac cagccacaaa aatacttcct
    89761 tctaaagcat agtaaacctt tccatttatg ccgtaaccaa ttgtcgtcaa taaattattt
    89821 ttggataatt gcggtttttc accggtattc atgacaataa atgaacctgt accataagta
    89881 ttcttgacca tgcctggttc gaatgccatt tgtccgaata atgcggcttg ctggtctcct
    89941 gccatccctg aaattggtgc ttcgcttcca taaaaatgat aaccttgcgt ataaccataa
    90001 acttctgagt tagaagtgac ttttggtaac atcactcgag gaatatttag taaatctaaa
    90061 atctcctgat cccaatctag ctgatggata ttgaacaaca tcgtccgact ggcattcgag
    90121 taatcagtaa catgtgcttg tccatcggtc agtttccata ctaaccaaga atcgatcgtt
    90181 ccaaacatca attcgccttt ttcagctcgt tcttgcgcgc cttctacatg atcaagaatc
    90241 cagcgtatct tcgttgctga gaaataggca tcgatcacta accctgtttt ttcgtggaaa
    90301 aattcttgat agccttcatt ttttaagtta tctgcaattc cagctgactg tctggattgc
    90361 caaacaactg catgatagat tggacgtcct gtatcttttt cccatattac tgttgtttca
    90421 cgctggttcg ttatgccaat cccagcaatc tgatttggtt tgatccctga ttcgatgaac
    90481 gctccggcaa taactgactg tacagaattc cagatttcat ttgcatcatg ttccacccaa
    90541 ctttcttgcg ggaaatattg cgtgaactct ttttgagaac tgccgatgtg tcttcctttc
    90601 ttgtcaaaga taattgctcg tgagctagtt gtaccttggt caatcgccat gatatacgtt
    90661 gattctgtca ttgtgattcc ctccattata tgcaaacgct ttctttaact actcttatca
    90721 tactactgtt ttaaaaaaag aaactgtcaa aattttattc tttttttctt tttttgacag
    90781 ttttttatta tgaagctatt ttttaatcaa ttcttttctt ttttcagcgt ctctattctt
    90841 cttttcaatc gaataatgtc gtaaggtgac gcaaattctg acagataata ttcacactgt
    90901 tttttgtttc tctggcaaaa tgtgatcacc aaatcatatt ctttaccttc ttcataactt
    90961 ccaatctcaa taccgccgat tcccctcagt tctctgatca gatattgttg aaaagcaata
    91021 cgataaatcg gcagactttc taaatcgatt ccaatcacta ctggttttgc aatataaaaa
    91081 tcgatcatca aaagaacatt ggcatactgg atcatcaggt aactaagctc actatcttca
    91141 gctcttttcc tcggcaattg ctgcaccgct gtttcctgta atttcgccag taactgtgtt
    91201 aaggaatgcc cgagcatttt ctgctgctgt tccaaaagcc gatcacgctc atagacttcg
    91261 atctttccaa caaagaaaaa ccattcattg ttgatctggg aaatttggta gcctactgcc
    91321 ttttcttctt tgattttcaa acgacgagga cgataatgcc ataacatcgt ttcccgaatg
    91381 taagtatcta aaacagcggt tggtaacttt ttgctccgtg tcaaatcata ctgataaaat
    91441 atttcttcgt ctactatcga aaaagaaaca aaaaagctgt aaaacatcat tgtttcgtat
    91501 acattcagtt ctgctgctgt tcgactaaga tgcatcacga tgattggatc gattgcttga
    91561 taaagccggt ccgactgata gagtaatttc ttttctttca aagttgcgta cgtacttttt
    91621 tcattcaata gtcttttttt cgtaatcccg agccagcaag caatctgtgc ttctgctgat
    91681 gccgtaaacg ttgtttctaa tacacggtcc aatcctagaa tgaacggtct tttttctggt
    91741 gtattttgca aaaaaagcgg tcgctgatct tccgggatat attggaatag ttcataataa
    91801 aaatatcgta tctgtagctc ttctccgtat aattgaccgt ttttgatttg taatccaaat
    91861 tcctctaaga gctggttcaa ttctttgatc tttcgaaaaa cagaagactc actgatatta
    91921 aacgctgtcg ctaatcgaac gatggatact tgctgatgtt ctaataaata gacgagcatc
    91981 ttaaacttca agctttcctg caataaaaac aatagaattt tttccaaact tacatcttcg
    92041 tccaacacaa gcagcgcctg atactcattt cgctgtactt ctactttttt ttccatcatt
    92101 cggccaatca attcaatatc ttccaaatat tgatcaaacg aggcttttga cagttggaca
    92161 tgctctctca aaccattgat agaggctgtt cccccttgca gaacaaggtt tcgcaaaatc
    92221 aggatctgta actgctcttt tctttctaat aatgcttcaa tcttcatagg cagtattcgc
    92281 cacctttgac cacaccatat ctggcacacg gtattctggc tggaaaaata gctcatgttc
    92341 ttctaactgt tcaaacgtcc atccttcttc tactgcaatt gtcagttggt caagaaggtg
    92401 gaacatctct tttttgcttt ggagctgtgc tccaagaatc tttttttcag aatcaaatag
    92461 aaatttgagt ttaactttgc tttgatttat tgctgtaagc ttgtagtcta cgtatcctgt
    92521 ttcaattttt tggggataaa aaatgctctc ttcttctgtc aaacctacgc ttcctaagaa
    92581 atagccaaac cactgattgc cgactggacg ataggttccc tgatcctttg tctgtgagcc
    92641 actaattgtc ttgctgacat ttcgtgctgt acggacagcg ttatttacta gagaaacata
    92701 cattttttca tttgttggac gaaaagttac ttggatcgca tcgccaatcg cataaacgtc
    92761 tttttgagac gtttgcaagt agtcatctac ccagatcgtt ccatcatcat tcaatgttaa
    92821 ttgttcttcc cacatctgat tgtcgggacg agaatgatta gccagcaaaa caagatcata
    92881 gttctccttt cttttttccg tctctacaat agcggtttca ttttcggtca aagacacaac
    92941 ttgttcttct aaaaaaagtg aaagcccttg atttttcatt tcctttacta acggttctgt
    93001 catttcagga tctagataac gaaaaagaat tgactttcgg ctttcgtaaa gatggatttg
    93061 tttttttctt gatgccaacc cttcagctat ttcgattcct gcttgtcctg caccaataat
    93121 tgcaattttc tttgcattca tgatcttttg ctgaagagcc gttacgtttt gtatcttttt
    93181 aactgggtga ataagcgttg attctgcaga aatatttcga aacttttgat ttgaaccagt
    93241 cgctagaatc aaacgatcaa aatccaattt ttttcctcct gctaaaacga cttttttttc
    93301 tttcaatcct attactgtct tttctgtgta aacatggatt cgatagcggt cagtcagttc
    93361 ttctttcgtg atccaagaaa gttgtttttc actggtaacc tttcctttta atatcaaatt
    93421 gaacccgctg ggaatccatc ctaacttcga ttgcttttca atcaaatata tttcacttgt
    93481 tggcatcaat tggcgaagat aaatagccgc atgaatccct gcaaacgacc cgccgacaat
    93541 tacaatgttc atctgcattc ctctttctta taaggtgttc tactgctaac ttctcaatca
    93601 gaaaacacag tattcataac tcgctttcaa ccttatgata catagcgatg cagcagaatc
    93661 aaccctctcc atcataccgt gttctaggtc tgcttctcaa taatgtacta gtggacctaa
    93721 ttgaagagta tcatttctga agagatgttt caaaaaatgt actcttataa aagaaaaaaa
    93781 ctcgcctttt acgtacctct gtccaaatag gaaacagaaa agaacgaaaa gacgagtttt
    93841 agcgtgtgta atgtaatagt tagaatagac acatcattta tttttaattc tttaatgctt
    93901 caacaactgc ttcgcctaat tgatgagcag attgaggatt ttgccctgta agaagccgcc
    93961 catcaatttg aaccacttct gaaaaagctg ctccagactg atagtttgct cctttttctt
    94021 tcaaagcatc ttctgctaag aatggtactt cattcgttgt accatttgct tcctcttctt
    94081 cattactgaa cccagtcact gtacgtccag ctaaaataga ttttccagac tcatctgtaa
    94141 ctggcaacaa tcctacaaca ccgtgacaaa cagctgagac cagcccttca tttcgataga
    94201 tttgtgaagc gactttccca agatctattg cttctggaaa atcccacatt gttccgtggc
    94261 caccagcata ataaaccgct gcataatcat ttgcatccac atcatcagga cgtaaggtat
    94321 ttcccaaggc ccgatttctg aaatcttcat cttcataata ggcccaatcg atttcatcca
    94381 tattcaaaac actttgcggt tctaaaggta catatccgcc tttaggactg acaaaatctg
    94441 catcgatccc attttttact aatacatcat aaaaatgagt cagttcactt aaccacaaac
    94501 ctgtaggacg ctcgatcttt tcgtactttt ctacatttgt taatacgatc aatactttca
    94561 ttttcccatc ctctttctat aaaaattgcg ttattgataa cgtgctttca agaaattata
    94621 taatgggctt ggcaaataaa gctctttata tgcttcttca aaaggtttcg ttacaaattc
    94681 atcctcacgg ttttccaata atttctctgc ccagtcagga tcgacgatca tttgctgtcc
    94741 aattgctgca agttctgcat atttcaatac ctgctctaca tcttcgcgat tgcggatccc
    94801 accgacacca actaaaggga tctttccttg aatcgtgtta tggaccactt caagaatcga
    94861 tttatcgttg tatttatcag aacgagctat tcgatcatac atatttaatg aaacatgcag
    94921 ataatctagt tttgactcac gtaatcgttc aagaagccag atcgtgtctt caaaacggat
    94981 acctggtgtt tcatattctt ctggtgagaa acgataccct acgatgaatg gttttttagc
    95041 atattgttca accgctgaga aaaccgttgt taataattct tcaataaaac gataacgatt
    95101 ttccaagcta ccgccccatt gatcctctcg tctattcgaa tgcggagaga aaaattgttg
    95161 gatcaaatac gtatttgctc catgcaactc gattccatca aatcctgatt caatcgctct
    95221 acgtgtggct ttgccaaatg cttggattgt ttcaacgatt tcttcttcac tcattgctcg
    95281 tggtgtttcc gcatcaggac gttttgctgc aactgcactt gcagatacag gctgttctcc
    95341 agataaagtt gctcgacttg tcattcgtcc accgtggaaa atctgaataa tcgcttttgc
    95401 gccttctcct tggattgctt tcgctagctc acttaatcga ggaatcattt catcatgagc
    95461 gatgctgagt tcacctggcc agccttttcc gatatcttgg acatttgcag caccagtaat
    95521 aacagcacca agacctttag aacgttgttt ataataagtg atctcatctt cggtgatcac
    95581 tccattgaaa aagctgagtt gtgtcgtcat aggcgacagc atcaatcggt tcttcaattg
    95641 aactcctgaa ggtaaagtaa ttgattgatc aaattgtgac attgtcattc ctcccataat
    95701 tcgctctgat taacctatcg agaatcatct atcattaaac cactcgatta gttgtacatg
    95761 caactaattt gcttaaaaaa ttttattttt tttgtaactt atctgttgca tcattcattg
    95821 tctgggctaa ttctttactt gcttcatttc ccaaaactgc tttgtatgac tgatgaagtt
    95881 cttcccagct accatcgact tccttagcaa attggcgtcc ttgttctgta agataaacta
    95941 atgacaagcg accttcgcta actgttttga cgtatccttt atattctaat ttttcaacaa
    96001 aacgagcaat cgttgatggg gcggaataga gcatttgtgc aatatctttt tgtggtattc
    96061 catctttttc ttttaatatc aataaaataa aaccataagt tggtgccaga cctgtccttc
    96121 taaacgcttc atcagctaat ttattcaaca tgcgatctag ctttttcacg ctaaagaaaa
    96181 gacattcatc taacgctttt tcaattggct tttccatttc atcccctcca ttggcgtaca
    96241 tatcatagta tgatttttat tttttgtcaa aaaacacgga ataatgtccg cctcctctgt
    96301 ctagatttga aaaaaagaag tagccatctt caaagaattt cggcttgttt tcgacaaaac
    96361 tccttctgga acaccgtgaa tcagaggttg tgaaaaagtc gtttagtttt gagtatgaag
    96421 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa taccgaaaaa
    96481 ctggcttttg aacaccgttt atatcagagg ttgtgaaaaa gccgtcctgc atcaagtaat
    96541 aagaacagcc aaacaaaaat agttcctcat attttgtcgt tttttgagct aatgccgatg
    96601 gtcagctgat tgtacaccgt ttattgagag gtcgtgagcc tgccgttgaa ctccgagtca
    96661 caaggagcaa tttcaaaaaa cagcttctca tgttttatga ctatggtgac ttgtgatcga
    96721 ggagttggca ggagaatact gtttattcgt aaagaagggg ctgtgacaag acttttgtca
    96781 cagtccctta tttcctttta ttataggatt tcgccatttg attggatgac ttgtctgtac
    96841 caatcaaatg attttttctt taaccgtttc tttgtgccat tcccttcgtc atcttgatca
    96901 acatagatat atccataacg ttttgacatt tgtgaagtag aagcgctgac gatatcaatg
    96961 cagccccacg acgtataacc aaataaatct actccatctt tgatagcttc cttcatctgc
    97021 tcaatatgct gcttcaaata gtcaatgcgg taatcgtctt gaacggttcc atcttcttct
    97081 actttgtcta tagctccgag tccgttttca gctatgaaca gtggtttttg atacctatca
    97141 tataattgat tcaaagcaat tctcaaaccc ttaggatcaa tctgccagcc ccattcactt
    97201 gatggaagat acggattttt aattcctcca gttattaagt ttccttggac cccttcccaa
    97261 ttttctggat gagcactgac tgcactagac atgtagtagc taaaagaaac aaaatcaaca
    97321 gggtaggctg ctaagatttc ttcatcacct acttctattt ttaagttgac acctttttct
    97381 tcaaacattc gttgcgtata cgctggataa tatcctcttg cttgaacatc actaaagaac
    97441 aaagaagttc tttgctgctc aattgttttt tgaatatcat ccggatcaca agaatacgga
    97501 acaagaagct gatagctgat catacaaccg acttgtgaac ctttgataat ttcatgacaa
    97561 gcttttgtag ctaaagcact tgctataaac tgatggtgtg caccttggta tttcgcttgt
    97621 tccaaatggg gatggttttc ttcaattact cctacgctaa caaaagggtg atgttttaca
    97681 cagttgatct cattgaaagt catccaatat tttactttat ttttatagcg tttgaatact
    97741 gtttcagcga aacgaacgta aaactgaata agctttcgat taggccatcc accatattct
    97801 gtaaccaaat ataacggcat ttcataatgg gaaagagaaa cgactggctc aatcccatat
    97861 tttagacatt cgtcaaacac ttgatcataa aaaaataatc ccgcttcatt gggtgtttct
    97921 tcatcaccat gaggaaaaat tcttgtccat gcgatagaca tccgatagca tttaaaaccc
    97981 atttcagcaa ataaacggat gtcttcttta aagtgatgat agaaatcatt tccgtgtctt
    98041 ttaggataat aatacttgct gttgggatcc tgcgccgctt ttacttgctg atgcgtcatc
    98101 atatgccatt gatctgtcca tttttcttta ggtaaatcgc tgtcgaatgt atacacatca
    98161 gacacagata atcctttttc atctacatcc catgcacctt cacattgatt ggccgctgtc
    98221 gctccgcccc ataaaaatcc ttctggaaat tttttcatat cgatccccct aaacttgcta
    98281 gttttcttgt ttgattattt ttcttatgac tagtaatgat atttttgaat ctactactat
    98341 ttgtaacaat aaccattgtg gtcggatcat atccctcttt gattaattct tgcaaatcta
    98401 cttccatcaa taaatcgcct ttacgtactt tttttccatc agatactgca ctttgaaagt
    98461 atttgccatt taaattcact gtatcaatac cgatatggat cagtatctct gctccttctt
    98521 cactgactac cccaattgca tgtttcgttg gaaaaactgt tgaaacttcc ccgtttacag
    98581 aactgtagat ttttccgtcc gttggaatga tcgcaaaccc ttctcctaaa gatccactag
    98641 caaatgttgg atcgtttact tcagataaat caattatttc acctgctgca ggagtactta
    98701 acactatatc ctttgaggat tcttcttcga tttcatcctt gtagccgaaa agaaaagtta
    98761 gcatacagga actgatcatc gctaagaaat aactagcaac tagccaaaca aatccttcac
    98821 caatataaat cgggaaagac aaaatacttc ttgtagcaac tgccaaagca ccggctcctg
    98881 cggcaccagc tactccacca aatatagctc cacatataca agcaatgtaa aatggtcgtt
    98941 tatatttaag agtcacacca taaacagcag gttcagtgat tccaaataat gctgtgataa
    99001 atgcagataa agataaactt tttactttct tatttttcgt tttcaaaaat accccaagta
    99061 ctgctcctgc ttgcgcagct acggcagtca tacatgctgg acctagcgta tcacgaccgt
    99121 agaaagaaag attgttggtc attactggta caagtcccca atgtaaccca aaaatcacta
    99181 acacttgcca aaatccgccg ataacagcac cagataaaac agggctaaag ttataaacag
    99241 ctaaatatcc agctgcaatc agctgacttg cataatctgc aattggacct acaactaata
    99301 aaataattgg gaccatcacg atgatacaaa tgaatggtgt caataaattt cgaatacttt
    99361 cgtgcaagct tttgcgaatc ttaggctcaa tataagaaag aacccataca gctaaaattg
    99421 ctggaataac cgtacttgta taccttgcta gaatcacggg catccctaga aatcgcaagg
    99481 ttatgctatc actatatgca ctgacaatcg taggataaac gagagctgct gcaataacca
    99541 tagcgatgaa acgatctgta ttgaactttt ttgcagcagt atatgccaaa aatataggca
    99601 agaaatagaa aaatgcatca gctgctgcat tcaaaatgat ataagttcct gattcagtag
    99661 ttaataaatt caaactcgta cataaggcga gtactccttt gatcaaacca cttcctgcca
    99721 agacgcctaa agtcggggca aaaacagcgg cgatcatgtc tataaaagag tttaaaatgc
    99781 cttttttctc agttcctctc tttccactag atgcatcatc ttctagtttc atttgtttgg
    99841 ataatacgtc atacacttca tttactgcat ttcctactac gacttgaaac attcctccag
    99901 cttcgactac agtgatcaca ccttctagtt gctctacctt ctccttatct gccttcttat
    99961 tatcttttag ggtaaagcgt aaccttgttg cacaatggta aacattgctg acattttctt
   100021 cgcctccgac aaatcggaca atatctttcg ctaattgtga atagtccatt tttacttccc
   100081 ccttgttaat taatgccgta gcaatccttc ttacaagata agtatagttc ttagaaaacg
   100141 gtttctccat tcaattagta ccccttctaa tagcgaacga aactgtacaa tagtgtgtat
   100201 ggtttactta gccgagcaaa tcactcactg cttgaaagaa aacaagtata tcgtttctct
   100261 tatagtcaaa ggagacaaaa aaaacacgct tgatcttcct ttgctgatgt ataagtggta
   100321 tctcaagctg atactctgtg atttttgttt ctacttggct tttgataaga cagcacaaca
   100381 aagtctcggc atttaacgga agcataacta ggtcagaact aggagagatt agttgctgaa
   100441 tggaatctat ctttttatgg atttttattt gattttcttc tttgtagtaa tgatcaagca
   100501 gttttgtttt ctttttttct tcaactatgg tcagcttttc acgcaaaatc tgataaatct
   100561 cccaagattc aaactcatct agaacactag gtagataaat caaatcatcc cactcaaaaa
   100621 taagctcttg ctcagtagtc aaaattgcat catattccgt tattttgttt gattgatttt
   100681 tgaaaagata cactggttct atccggcatt ctttgatcca catgttcata ctttgttgaa
   100741 tctgtttttt tagtgtattc aaaatactaa ttggctggtt actaacaatc agtatctccc
   100801 cttgttttcg aaaaggattc aacgcttcgg caagatacaa aacaaaataa gcgctttcag
   100861 ctaaattggg acgtaattga aaatgtattg ggaagaaacg ccgaattaaa taaatttcta
   100921 aaggataacg ggagatgatc ttttgtacaa aatgattcat ggtattatgt ccggcatcca
   100981 tcctcgtttt catttgtttg atatgggtta gcaaaaattc tgtttcatgg aacaataacg
   101041 aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa tgctcttaga ttcttctcta
   101101 tttctttgtt tttttcatca ttaatcatca aataatttaa ctctaatagt cgattttcaa
   101161 ttgcttgttt ttcttgattg gaaaaagaat agggtagttc tgatgataac ttttttgtga
   101221 gggtctctac catcggaata aataatgtat acctgtttgt tttttctaaa agttcttctc
   101281 ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat gaagtcctct cctgaaacaa
   101341 tataattata tgatataaga acctctttta atgtcttttg gacagtcggc atgatattgg
   101401 aaaaaattgt actgatttgt tgaggcaacc ttgcgtgttg gatcaccgtt tctgttccaa
   101461 caagagataa gaaaattctt tttgaatttt cttcagcaat gatttttagt ccttttgtcg
   101521 acgaaacttc cagttctaat ttaggattgc gctcgatcca acgcaaaatc gtttttattt
   101581 gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc tatcttttgc atactgacat
   101641 accctttttg aaacaataag tacgtcacaa tagttgaagc tcggaaatcc atgctggaat
   101701 agattttatg tttcgtcacc tcttgtatga tttgctgttt agtttcagat tcaattgatt
   101761 ctaaaaaata acctgaacgt ttatttgact ggataatgat tttcttctcg tagatagcaa
   101821 gcatattttt gatttcagtt tggatcgttt tcttgttggt acctaaagct tcagctaatt
   101881 cttcgcttgt tatccaatga ttcttatttc ttaatactac aagaatttgg agttgtcgtt
   101941 tatttaagtc catttgctta cacctcctct ttgttataaa catacaggaa aaacctctca
   102001 aatgacaata gtcacttgca gaggtttaaa aatcatttga agaaagatct atattcaagg
   102061 tttttaccat tagactggat aatttgttta taatattcaa aactatcttt tttcaatctc
   102121 ctaaagcttc cgctcccatc attttgtcta tccacataaa taaagccata tcgcttcctc
   102181 atttcccctg ttcctgccga tacgatatcg attggtcccc accataagta accaatgatt
   102241 tggcaaccat cttttattgc ctcagcaatt tcctgaagat gtatttttag atactcacaa
   102301 cgttttggat cgtggattcg attcaattca tctggtgctt catctagtcc aatgccattt
   102361 tcaactatga ataatggtaa ttcataacgg tctgtcaatt cattcatgac ataacgaaac
   102421 cctagaggat caattggcca actccaaggt tcgggagatt tttccttcaa aaagggatta
   102481 tcgaaaccat ttgctccgcc agtatctacg cgcatttctg cttctttttt atagacagcg
   102541 ctacgataat aactaaatgc aatataatct gccgtgttag cttgcaagca ctgttcgtct
   102601 ccatctaaca ttactggtgc acagtcttct tccttccatg tcctgtaaat ataagctgga
   102661 taatgccctc ggcacatgac atcggagaaa aaccatgaat tgcgtttaaa ttgcagtgaa
   102721 ccaaatacat tctctggatc acagtcatat gcataagtcg ctaatgcaga aaagctacac
   102781 ataataccca tcttagcatt tggatcgatt ttctttaata attgagtagc acgggcgttc
   102841 gctagaaaca tatgatgaca ggcttgataa atcacttttt ttgttagtgt agcaatgggt
   102901 tcaagtttat ctctagcagg gaatgtaacc aatccagcag ctgcataagg catacgaaaa
   102961 gcattattga tctcattaaa ggtcatccaa tatttcactt tcccttggta gcgttcaaag
   103021 acggtctgcg cataaaattc aaaaaagtcg attaattttc gattttgcca ccccccatac
   103081 tctcctacca atgctaaggg ggtttcataa tgactaagag taattactgg ctcgatatca
   103141 tatcgattta atgtattgat tagtctgtca taaaatacga gaccagcctc atttggcttc
   103201 tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc ttgttcgaaa agcttttaaa
   103261 cctaactcct tcagtaaacg aatatcctct tcaaatcgat gataaaaatc aatcgcttca
   103321 tgagaaagat aatggagtga ttcgtctaaa gcaatctccc aaatcttttt ttcttcattc
   103381 cactgaatac taggatgctg gttcatgatc ccgataagcg tatctgtcac attaggtaat
   103441 ttgccgtctt ccaaccatgc accttcacat tgattagcag ctactgctcc gccccataaa
   103501 aaattttctg gaaatatttc ctgatccata actatctctc ctcgctatca acaatttctt
   103561 acttgaagta tagctcgatt catcaaaaca agatattcta actatttcgg cagaagagaa
   103621 gaaaaaaatt gaatgctact cttcattttt aatatttata tataacaaat acataagacg
   103681 atccccgaaa gaaaacactc gttaaccatc taagcaaacg atttatttta cttccaacac
   103741 aaatgcttca taaggtcgta aatcaacttt gccgtctggt aactgtttca gaggataatt
   103801 tgacacaata acccttaaag cttcacgtgg aatagtgaac gtctgctttt cactggacat
   103861 attacaaata acgagccatt gctggtcttc atatatgcga gaataggcaa agacatgctc
   103921 aggcgtcttt tccactaatt catagtctcc ccagacgatg atgtcatgtt ttctacgcaa
   103981 atcgatcaat ttcttgtaca tataaaaaac agaatcttga tctgctaacg cttcttctgc
   104041 attgatttct ttataatttg gattgactcg aagccatgga attccagtag taaacccagc
   104101 atgttcacgg gcgttccatt gcataggcgt cctagcattg tctcttcctt tcgccttgat
   104161 tgattccaag atttcctctt ttgtaaatcc agaagaaatc ctttcgttat acatattgat
   104221 tgtctcaata tcttttgctt ccgcgatatc atcgattggg caattcgtca tgcctaactc
   104281 ttctccttga tagatataag gggtgccttt catcatatgg agcaagatag caaacatctt
   104341 ggcactttct tttcgataca catcgtcatt tccccaacga gaaacaattc gcggaagatc
   104401 gtgattattc caaaataaac tattccatcc ttcatcacct aaagatgttt gccatttgga
   104461 taatactttt ttcagttcag agatactcaa gggtttcaaa tcccatttgt ctttgccttc
   104521 ttgctgatct aatcctatgt gttcgaattg gaagaccatg gataattcat ttcttttagg
   104581 attggaataa agtttagcga tttcggtagt tgctccccat gtttcgccaa cagtcatcag
   104641 atttttgtca ccaaaagttg cttgattcat ttcctgaaga tactcatgca gtttaggtcc
   104701 attacttgtg atttctttat ccggttcttt gccaatcata tcgatcacgt ccatacgaaa
   104761 gcccccaata cctttatcga tccagaaatt catcatttga taaatctctt ggcgaacctg
   104821 tttatttttc caattcaaat caggttgacg cttgctatac aagtgcagaa aatattgccc
   104881 cgttttttca tccaactgcc aagcagaacc actgaatatt gagcgcaatc tattaggctc
   104941 ttttccattg accggatcac gccaaatata atagtcccga taaggatttt ctttattttt
   105001 acgggcctca atgaaccacg gatgttcatc agaagtatga ttcaccacca gatccatgat
   105061 gatacggata tttcttttct ttgcttcttc aatcagttct tccatttctt ccatcgtacc
   105121 gaattctgcc atgattgctt gataattact gatatcatac ccattgtcat cattcggaga
   105181 ctcgtatact ggagaaagcc aaattgcatc aatacctaaa tacgccagat aatccaacct
   105241 ggaaataatc ccccgcaaat ctccaatacc atcttgattt gaatcttgaa aacttcttgg
   105301 atagatttga taaacaactg aatggtgcca ccatttttgt tccatgattt tcctcctata
   105361 aatggatgtt tctatttttt gcttaatcat ttattttagt ctctctcact aaaaattgta
   105421 acgctttcaa atagctattg caccttgtta tcggtaacag aataaaacaa aaaaacagct
   105481 gaggaaattc cctcaactgt tttttgaaag gattgattta tgcagggaag aatgctgatt
   105541 tcacaacttc tttataaacg gtgttcactc agctgtccct tggtattagc tcaaaaaacg
   105601 gcaaaatata agaaactatt ttttcgtttt tttctctaat actcaggcca gaacgctgat
   105661 ttcacaacct ctttatttca cattcaatat ccacatgaat cccacaaata caaagaacag
   105721 accatacatg attggagata cttcttttcc tcttttagct gctaacatgg taattggata
   105781 gaagataaaa cctaatgcaa tcccatctga aatactataa gtcaaaggca tgccgatcaa
   105841 gatcaagaat gaagggatcg caatctctaa gtttccccat tcgatttgtc gcaaagattg
   105901 tgccatcaat actcctacca taatcaatgc tggtgcagtt acttgtgaag taacaactga
   105961 tagtaatgga gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat
   106021 acctgaacgt cctccaacgg cgataccagc tgatgattct acaaatgcac caacaggaga
   106081 agtaccaaat aaggaacccg caagcatagc tgttgaatca gaagctaaag ctttccctac
   106141 acgaggcatc ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc
   106201 tgctgtatca aagaaagtca caagcaagaa tgtcagcact acgacccaca tttgaagaga
   106261 attgatgtcc cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc
   106321 ggcaacaatt tcagaaggca ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc
   106381 tgccatcccg atgaagatgc cacctggtac acgccgaaca agcaagatag cagttacgac
   106441 taatccaaaa atcgtcaacc aagtagaacc aacgttgagt gatcctaatc ctactaatgt
   106501 agattcattc gaaacgatga tcccaccttc gctcagacca aggaaagcga taaatagacc
   106561 gataccgcca gaaatcgcat atttcaaatc tgctgggatc gcatcaatga tcatttcccg
   106621 cagcttgaag attgtgatca aaataaaaat caaagaagca acgaatacgc cggctaatgc
   106681 tgtttcccat ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag cattgatccc
   106741 aagagcaggt gccgttccaa ttggataacg ggctaatacc cccattaaaa cacagccgat
   106801 cgcactggct aatgcagtcg cagtaaacac tgctccttca tccattccag aagcacctaa
   106861 tacagttgga tttacaaata agatataagc cattgagata aatgttgtaa atcctgccag
   106921 tacctcacgt tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa gtattttttc
   106981 cttcatcttc catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt
   107041 tattaataaa acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt
   107101 cggattttaa gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat
   107161 gaacattcac tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta
   107221 gtctcaaaaa tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga
   107281 attcttttgt atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt
   107341 ttccccagcc ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac
   107401 tatgttttgc tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt
   107461 ttcctccttt ttattcaatc agacgactca gcttttccta taacaaaata attgccttca
   107521 gggtcagaaa aattgaaagt caagatgcca ttgttatctg tgatttctcc tggttcttcc
   107581 atgttctcgt agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct
   107641 aatacctctg gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct
   107701 tttgagatgc tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt
   107761 tctgcaccaa ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc
   107821 atcacacgtg ttttttgtat catcatttga cccttctttc tatttgattt tctttcctgt
   107881 ctataaactg attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa
   107941 gaaaaataaa gcaatagttt ctaggattgc tttcctccac aaactttttt tacaaaaaaa
   108001 aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc
   108061 atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt
   108121 caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac
   108181 cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag
   108241 acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc
   108301 atcggaatct gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag
   108361 atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat
   108421 actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg
   108481 tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc
   108541 ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct
   108601 tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac
   108661 gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca
   108721 gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc
   108781 ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat
   108841 ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg
   108901 agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat
   108961 gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa
   109021 tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc
   109081 tcttgatcga tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt
   109141 ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt
   109201 ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc
   109261 gtcatttttc gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg
   109321 ccttttttga caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat
   109381 tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg
   109441 ttttctcgaa tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg
   109501 atttttttat tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg
   109561 attgctcggg cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg
   109621 attccaggat tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact
   109681 tctttgaatt cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga
   109741 aggatttcag ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt
   109801 gttgcaaatg caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt
   109861 atattctttt cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta
   109921 aaataagtaa ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac
   109981 taaaaaatcc catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga
   110041 tcgcacaagc aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat
   110101 tcgtatcaaa aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga
   110161 cttctttacg cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg
   110221 accattcttt cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg
   110281 cacgaacatc tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt
   110341 cttcgaaagt ttcttttcct atttcactga ttagttccaa tgaaatcgtc cggatctcct
   110401 caaaacgctc tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga
   110461 gtagtctttt gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg
   110521 tatgactatg taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa
   110581 tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg
   110641 ggctgtgaat aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa
   110701 aaatatgacg agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc
   110761 acaacctccc cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc
   110821 atcaaacatg agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag
   110881 gctcacgacc tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct
   110941 ttgttactta ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac
   111001 ttgagtatag tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt
   111061 tcggtcggcg atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg
   111121 atcttccaat acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga
   111181 ttcacgatca cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat
   111241 cgtgatcacc tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc
   111301 actaaaagga tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc
   111361 tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt
   111421 aaacccgatg gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc
   111481 tggaaatcgt tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc
   111541 cagaagaatg aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt
   111601 tcataaacag tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca
   111661 cattatggcg aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg
   111721 tccagttgac taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt
   111781 caggataatc ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta
   111841 ctgcatcgat ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat
   111901 gattcgtacc gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg
   111961 cgtcttccgg tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac
   112021 cgccgattgt tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta
   112081 ctgcttttgt cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag
   112141 aagtatatcc gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt
   112201 gcaagactgc atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat
   112261 gcatgccgat atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt
   112321 cttttgctgc gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa
   112381 ctgtaaaatt caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt
   112441 attgccacct tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc
   112501 atctcatcca ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca
   112561 tctgcacgct tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt
   112621 ttattttcca aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa
   112681 aatttaagtc caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa
   112741 atctcttgtt tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg
   112801 gctttttctt cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca
   112861 aacatcattt tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca
   112921 tataagagtg ctgcggctac tgtatctaca tcggttgctg tagcattgat gaatcccatc
   112981 gaattaaaga tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata
   113041 ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct
   113101 ccggcaaaga aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa
   113161 gtaaacatgg caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt
   113221 gggctataag gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc
   113281 atagctatcc ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc
   113341 aatacataca cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt
   113401 tgatctcctg ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat
   113461 agcgggacca tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc
   113521 ggcaacttga tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca
   113581 acaccggctt caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat
   113641 ccagtgattt tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct
   113701 ccgataaaat caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag
   113761 actgtcgtca tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat
   113821 tttgtgaaac gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata
   113881 tctctaccta aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg
   113941 atcccttgca tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg
   114001 atgatgctcg aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag
   114061 atgacttgtc ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg
   114121 gtgatcgatg cctgagtcaa aatattttcg atgataaaat tgataacgcc catgttttta
   114181 ccctcttttc tttttgtatt tagataactc ccttatcttg cagaactggg gtgatttttt
   114241 cttcaatttc attttttgaa acaatatttt tcaaatagat catcgccgtt ttaggatcaa
   114301 ctgcgaactt ttcgaactgc gaacgaaaat tttctgctgt gatgatcaca tctggtttca
   114361 tggaaactgc ggaagaaata tcacaatggt ccagtttggc ttctactcct aatttgttca
   114421 aaacatcctc agcagacatc tgtgctgcaa aactacttcc taaacctgca ccgcatacga
   114481 ataaaatgtt caatttgttc atctttgttc tcccccgtat catttattat ttctcaacta
   114541 ctgaaaaaag cagttgtttg aaatcttcga tatttgtgca ttcaatcaat cgatccaact
   114601 tctcttcgtt gttgatcaat tcaattagtt ctttcatgat gttcagatgc gagtaggagt
   114661 ctactgctgc caaacaaaaa ataatcttta ctggatccat ttcaggatta ccgaaatcga
   114721 ttggttgctc aatcgtagct acgctgactc ctaatttatt gaccccatct tctggtcggg
   114781 catgggctag tgccatgtgt ttgccaatca cgatatacgg tccgtaatca ttcactgcac
   114841 gaatcatcgc ttcgacatag cgctcttcga caatgttttc tttaaccaat ggtctagcag
   114901 ctagttcaat cgattcctcc caagtagtgg ctttttcttt gatcatgata ttgctgtccg
   114961 tcaaaatatc ttttaacata ggctggatct cccttttgtt gatttctaaa ttgtttgttt
   115021 cgaataattt ttctaacttg ccatagatga ttcgatctac tcggccacca ctttctttca
   115081 tgacatctat tatttgagtg aataactccg tcgattcatg actgttaggc tctaaccgct
   115141 gtagttcttc atttcttttc aaaaagtctt ctataaccaa gatattcgtc tcagttaaaa
   115201 tcggatcgac aactaaaagc ggcttatttc gataattcaa ttgaaaggtg ctgaaaacca
   115261 gattgacatc caatttatct actaattcga tttcttttga acttaaaact gcagtaatct
   115321 cgatctgagg aaacttttct ttcagattct ctgctaacag atttccagta gccatcccat
   115381 ggttacagac aacgactgat ttataaacat aattcaagtc tcgcttgatt acactggcga
   115441 tcgtcgaaaa gtggatgaca agaaaagcta tctcgtcctc cagtatcttg ccaccaatta
   115501 cttcttcgat cgtaggaaca aaatgagtga ctgcgcggta gattgggaaa taatttgttc
   115561 ggatgttttc tttcaatgga ttcatgactt ggatatgatt cttgattctt acaatcagtg
   115621 cagccatgtg cttatagaga ttttcgaata aagcttcttc ctttagatgg aaaggtacgt
   115681 gtgtctcgtt ctctacaaat tggatcaatt ggatcgttag taattgagct tgtacccact
   115741 cgattgaatt ggagatatct ctagcattga acgtctcaat agtgaaacga agataatttc
   115801 tttctacttg cgggacattg tccaattgaa attcctcgat catgtggtca ataaatgcag
   115861 aaaagctact gtcttttatt tctgtatttt tccaattaat ggcagagata aattcatgtt
   115921 tcctgattcg ctggatccag atcaatgtaa aaagcaggat ttgtttttta taaatatcat
   115981 cttcttgttt caacaactta ttggaataga tctcctgtat ttttttgatt tccaccaaag
   116041 gaaaataatg gagaaagatt ttttgagccg gtgttaattg tgcatcttga ttcataaaga
   116101 agtcggtcat tcccagattc tgattgataa aatcatagat catcgtacgg atagatcgtt
   116161 ctgaaccttt ataaaccagt ccttgctttc cataactgac gatctctatc ccataactga
   116221 ccagtgttgc tcgtaaccgt ctcatatctt catctaatgt acttttagag atttgaaatt
   116281 cttcttcttt tttgtttaga tggatcggga ttcggctgta agcaacggct aaaactagat
   116341 caaaaatacg ttcgcgccga ttcaaaaagc cgtctttcac atcttttatt tcagtttcta
   116401 atcgagcttg cttttccttt tccagcttca acaaaaaacc tttgttgcga attgtacgga
   116461 tctccggaag ctttttactg ctcaaccaga tattcgcttc aagaacttcc tgacggatcg
   116521 tccgctgact gacattgaac tcacttgcta gatgttttgc ttttactgga tattgggctg
   116581 tcatgattct gtgaagtatt tcaattgttc ttgttttcat agaaatgatc tccttctgat
   116641 ttcaagtata tggttccgct tacataataa aaagtagata tttctgccac ttgaagtgga
   116701 aagaaagtaa gcaaagcaaa caaaaaataa gccatatgct ttttcatctc ctcaacgaaa
   116761 aagacatacg cctattatta caaaaatttt gcccatctat acaaaaaaga ggttgtgaaa
   116821 aagctgtcct gcatcaagta ataagaacaa ctaaactaaa atagtgcttc ctatttttcg
   116881 tttggttgtt cttattatcg cagatgcaag cttttaaaca ccatttagtg gagaggttgt
   116941 gacagagatg ttcagccttg agcgataaga agcgattttg aaaaatcgct tcccatattt
   117001 tacaaaatcg tgccttattg ccgataggct atctctggca caccgtttat tcagaaaaag
   117061 gagctgcgac aaggcttttg tcacaactcc gttcataaat atttcctgtc aatccagcat
   117121 aatcggctga ataaaatcgg cttgccatct tttttctttc tcagaaaaat caaaatacca
   117181 tatctgagaa aaatactgtc gttgtgtctt acttcctgag atcaatcgcc catcttttcg
   117241 atcgactgaa taatcacagc atctgaaatc aattcggatg gcaaaactat tttcgtggat
   117301 ctgtcgataa tttcctactt cctgaatagt tattttttta gtatgatttc ttacaccgtc
   117361 tgctttgttt ttttgcagaa ctttctgatg ttcggcaaac agcttgtctg tataacagct
   117421 tctggctttt tcaggagttt cttcttccca agcggattgg atcttcatga atatcttttg
   117481 gatttccaga aactgtgctt tcttttcttt agccgttcct gacatgcggg acaatatctc
   117541 cgctttcagc cttttctttt gtcttttatt cttgacgata gaaaaagcag aaacccctcc
   117601 tactgacaaa acagctaatg caagaaatcc cgtttgctca taactagaag gacctgtacc
   117661 tggaccataa tacccataag aatctctgcc gtaagaacga ctgccaaaat aactgccccc
   117721 atatgaacga ccaccaaatg aacctctgcc cgaacttcga ccgctagatg tacctccacc
   117781 atgaccaccc gcgacagcga atccttgggt cggcagagca aacaaacata ggcccactaa
   117841 caaaatgatt gctaaataat tgattttttt cattgtcttc ctcgttatct tttattacaa
   117901 aaaactattt ttatgatagc cttgctaaaa ggaagagtca ataaccagat gcataaactg
   117961 tttttttagc aattttgtct tatttagtca ttgagtaaca tcggacaaat gtatgtgtcg
   118021 gcagtccata attcctctat cttcagcatg aacaagcttg cagacaagag atctgcgctt
   118081 cctccaggag agatttggtt ttcctgacaa aagtgcaaca gtgctgtaaa tggacgattt
   118141 ggtaaagcac tttgactagc agcatgagca agtttttgca tttccactaa cgttttctga
   118201 tcactgcgat gcaatacgca tgtgtcatct agcgaactca tcaaaactaa caacatatgc
   118261 atttgtttct gtacatatga atcattatct agctccttag ctgcttgaat tgcaagcctg
   118321 atatgcggat accctaatat cgcttcttca tatgcaccat ttacgccata tttttgcttc
   118381 accctatgac catgagtggt ttttttatga cgatattttg tatcaggaaa agcagcaagc
   118441 ttgctgacat catcaagtat tttcgataaa gaaaaaggct gttccatact gattttttgt
   118501 gcaatgacac tgatcaatag ccccattgcc caaatcgctc ctttatgggt attgatgcca
   118561 tttgtagcaa tcagcattgc cttttccgct tttcgcccaa tctcagcaat tctttctctt
   118621 aactcctgat caatagcatg atgccatgct atttgagcca tttcttcaaa aaaaggagtt
   118681 agcgtcttag cagacttaag aaataaagca tagtccatat cggaatgtgc acccgcatcc
   118741 agtgcatcca ctaatccagg ttttggactt aatgttactt ctgcgatcaa tgcttcctct
   118801 accaacgcag cgagttgctg agtcactgct ttttttcgtt cgtcatttat catgacactc
   118861 actccttctt cctactatta cttgctgaaa ccttttttga tagctaccca aatgatatga
   118921 agtaaccagc ttaaagcaaa tgcacaactt gccgtcatta gataagctac aagaatcatg
   118981 actcctgaag gtaactgcag ccaactatct ttaaacaata ataacagtac ttggaaaaca
   119041 aatacattgg ctaaatacgt acgataagca taaacagaca accatttgaa caatggcaat
   119101 tttggcgaat cgtttacaat cattttggaa gcgcctatga agacagcaaa gatgatgact
   119161 aaactgtata aactttggat cgtattcaag taaggtgcat tcgcaaagct caagtctcct
   119221 ggatgtgcga gcaatgaacg gacagaaaag aacgcgataa ctaaggcaac aggtaagaaa
   119281 agataacgta ctctgtccaa atattgatag actttttcgt gataaacaaa tgcggctacc
   119341 ccataaatca tgaaactagc aacaaaccga tctaataagt accagctgtc atgataaggc
   119401 ccagtcaata cttgtgtttg atagaacaga taccatgcga catataaaat cgttgaacca
   119461 atcaatactg gccatacata ttttttatta gcaaatactt tgtaaccgag ccaaacaaaa
   119521 aacggcatga acaattggat ctgcagcatc ataacggtat accataaatg aggcgctcca
   119581 tcacctgtga catatttcag taaaaagctt gcagtatccg tatacggagt ttgttgttgg
   119641 actgctggaa atagccaaag ataagccgtc gtccatagaa gatacggaac gactagttca
   119701 aaaaactttt ctttcaaaaa ttcccaataa gacaaatgct gacttgtttt taccatatta
   119761 taaacaattg caaaaataaa cattggtgct gtgtatttaa cgatgacgta aaaggtacca
   119821 agaaaagctg tatttgttgc gctgtggctg tttgccaaag caaagctcaa tatcgtttgc
   119881 atcataacag ccgttacagc tgatactttc tggaagtcac ttacgttgat tttgattttt
   119941 ttgttttcaa acatctcttt ctctctcttt ttctttatta ccaaagttct ttggttaatt
   120001 taggtccttc gactgttttc aatagaattt ttttctctgg ctctctcaaa tactctttta
   120061 gtgaaaaacc gccttgccga gtcactactt gggtatcgaa ttgctcaaaa aaagcaggat
   120121 gcgattgaat tacctgaatt ggcaattcaa tcggtgaatt tgcgtacagc agcaaatcaa
   120181 aatcactggt ttcttttact gccggaatcc cagtcgataa ttcaaatcct aagctgccgc
   120241 cgattcccca tttacatcct gataatatgt ttctagcagc atgaaattgc tgataaacag
   120301 gataatccat gattttctct tgtctatacg attcttgttc gatgatgtcc cacggatgaa
   120361 tgacttgttt gacggcatgt tttgagattt ctgttgacca tctctgattg cgggcaggtc
   120421 cacgtaaacc aatcggtagt atttctgcgt gatccgtcga cgcacgacga actgttccga
   120481 acatcttttc agttagaacg acccattcag gcaaggtgaa cccttctatt tcatgtttat
   120541 caaaaaggac aatatcatgt gcttgaatca ttttttatgc atcccattct tcgtccattt
   120601 tggcacggac tttatttgtt tcgacacgtc cgaatgtttt ggctgtttcg tttgtgtatc
   120661 gatttgtcag catcgtgtca tttgtattgc ggacatcttg aatggcttca ttgattgctt
   120721 cgatgacttt gctgacattt tgttctgaag cagcttggtc ggtgatatct tctaggaaat
   120781 gatagatcgc tcctagtttt gtataattat tgatatcgta agcgatagct ggaacttttt
   120841 cagcagctgc ttcgatttca gcaaccgaac gttttgtcac acgtgcagca ctttctttag
   120901 acatcgcttg gacggtaatt tctttattgt ttaaagcaat caagcggctt gattgtaatc
   120961 catgtgctaa aaatgcacct gaaatagcat ttcctgcaat caatccaata actggatgtc
   121021 cagcttgacg gagttttgca taagcagcag cactattagc caaagccaca tggataccga
   121081 tcaattcttc gttatagccg tatgcttggc ttggtacatc gatcacaacg atgattggcc
   121141 gtttcacttt tttgtcttta tcttcttcgt aaacgtgatc caaaatttgt cccatgcgga
   121201 aaccttcaac taaaccaact tcaccgtttc gtacacggta catcgggttt ttaggatcag
   121261 gtacgattga tgtcacgatg gcttcttcta tgaatttact tgatttacca taatacacag
   121321 tactaataga agaagttgga tttttcatgc cagttagtgc cgcaaaccaa tcgtaaccaa
   121381 cacttgctgc agctggttca tttccttctg ctacttctgg taagtcaatt gattttgctt
   121441 gatggttagc aattgcttca ttgtaatcgt caatggccat tggttttgaa agatctaaag
   121501 aatcaagtaa tgataaataa aatgctgagc tttcactgcg atgcgtgtct tttcgttcgt
   121561 ccattgctgc gatgacagca gctttgattg cttctacact atcagtcact aattcatcaa
   121621 tgatttttga ttctacccgt tgacgagatc ctaatgtatt ccaaatcaag tctttgtcac
   121681 tagaatcaaa ttctcgaacg cctgcttctt gttcaataac ttctggtcca ttcaaaccga
   121741 ctctcgcttt ttttgttgcg attaaataac tcattaatgc tgaagtaatc gacataccgc
   121801 caaatgatcc tacacgtcct ggaatcaaac cgatgactgg tacatattgt tttagagcaa
   121861 tgattacatt tccgatttct gaaatagata acagtccgta gtttgcttct tgcaaacgaa
   121921 caccacctgt atctagtaca atgactggaa agatctcatt tcctttttta ttttcttcta
   121981 acacatgttc aagtgcagca acgatttttg caccggaaac ttcaccgata ccgccacctt
   122041 ggaattttcc ttccatagcg attactactg cttgtttgcc attgatcgtg ccacggctga
   122101 caacgatccc gtcatcactt tcagggacga ttccttgtgc tggtaaactt ggcgcgatga
   122161 tttgatcgaa tggatcaatc aattcacgtc cattgtcaag tagagcgttc actcgttctc
   122221 tggcgtttaa ttctacaaaa ctatttttca tctgaaagtt cctccattgc ttgcgtcaat
   122281 cgtaggtaca caactcctgg tgtagcaccg aagtcattga tcacgatatc tgctgaaatt
   122341 ggataacgat caaaaaatct cgttaataca ttgttccata cttcgccgaa gccatcgctt
   122401 cctgtacaaa tttttacagt agattctgtt ttttctgttg gaaacatcaa gatttccaaa
   122461 tctcctgaac cgaccactcc tacatggatt ttcttattga tcggctgttt cgttggatat
   122521 ttataagcta attgttccat ggatctctcc cctttttgta aaacagagca tacacgttct
   122581 gcttattcgt ctttcttctt ccatctgggt aaatggcgac aaaaggtttt tctcgccaat
   122641 tttatgacca agttctgaat ttcattggtg gttcatacag gccatctgac cattcgatca
   122701 aatcatcgat ggtttgtgca gctaataatg aacgtttcgc ttcactacgt ttgatgccta
   122761 gatcttctgg taatttcacg atgccggctg cacgcaagct atctagtgat tctttcgtgc
   122821 tagtcattcc aagaggtgta acaccgccaa ttgctgcaat tgctgcttgt cgttcttctt
   122881 ttgtttttgc tttgtacaaa taagcaattc cttcttctgt cacgatatgt gtagtatctt
   122941 cgctataaat catcactggt gcattggcta agcctgcttg tttttgaaca gctagtgcat
   123001 ctaattcttc gacaaagact ggttttttat ttgaaccata tgtttcaacc atttgaacaa
   123061 ctaatttttg gcctttccct aatggatcat cattgtcgcg aagtgattgc catgctggtg
   123121 tagaatgacg acgaccgcca ggattatgtc ccatgttagg cgcaccgcca aaaccagata
   123181 aacgtccttt tgttacggta gaagagtttc cttcgtaatc taattgcaag gttgaaccga
   123241 taaacatatc gatggcatat tgtcctgcag cttgtgataa tgttcggtta ctgcgcatcg
   123301 tgccgtcttt accgacaaaa aagacatctg ggcgagcagc aatatatttt tccataccaa
   123361 cttcgccacc aaaactatgg atgctttcaa cccaacctga ttcgatggct ggaatcatcg
   123421 ttggatgtgg gttcaatacc cagttcttcg cgattttccc ttttaaccct agtgattcac
   123481 cataagttgg cagtaacaat tcgatagctg ctgtattgaa tccaataccg tgattcaaag
   123541 attgtacgcc gtgttctgcg tagattcctt tgattgccat cattgccatt agaatttgca
   123601 attctgtgat gttttgagga tctcgtgtga ataaagcttc taattgataa ggtttatctg
   123661 ctttcacgac aacgtctacc cagtcaccag ggatatctac acgtggtaaa gaatcgacaa
   123721 cttcgttgac ttgaacgatc acgataccat ctttgaaagc tgcagcttcg atgattgttg
   123781 gtgtttcttc cgtattgaaa cctgtgtaca agttgccgtc tttatctgct ttatctgcag
   123841 ctactaaaac aacatttgga atcaaatcga tgaataagcg tccataaagt tccaaataag
   123901 tatggatatc acctaatttc attgtgccgt cttcaatcat ttgggcgaca cgcaagcttt
   123961 gtgcaccagc ataagagaaa tcgatcttgc ttgcgattcc tttttcaaaa atattcaaat
   124021 gttctggtcg tgagatacta gacatgatca aatgcagatc attcactttt tctgcatcta
   124081 ctttttcaag actttctgaa agaaaacttg cttgtttttg attgtcgcct tctaatacaa
   124141 ctttgtcgcc gggagcaatc agtttttcca ttacttctac gattttttca gtttctacat
   124201 acttgccatt gagcaagttg ctgatttttt ccaatcgctg cgcttttttt tcgcgtttag
   124261 aagcccagtt gcggtttggt ttttccataa ttagtcatct ccatttttga tatagaaacg
   124321 tggcgtcatc aataccatgt tggtctagat tgatcgtacg gatcccttta tctacaccaa
   124381 attttgtatg aatcagcttt gtcaaagtat tgccgggggg aaattcaatc gtcagatcca
   124441 ttcctaattc ttttgccaag tccatcattt ggttccactg cacagggaaa gctaggtttt
   124501 ccaacaaatc tttacgaatc tcttctgcgt cctgagtggc tcttccatcc gtatttttta
   124561 tatacaaaca tctagcatca gatagatgga cagactgcat atacggcttg aatttcttga
   124621 ccgtatcatt catcaagaga cagtgagatg gcacagggac tttcagcagc ttcgctgttt
   124681 gcgcttggtg ccgtttggct tctgccaacg taacggataa accttcgata ctcccagata
   124741 acgtatgttg catcggacaa ttttcatttg acagataaac tggatggtca ggatcaaatg
   124801 tttttgctac aatttcctca gcttcgcttc tcgtcacacc tactactacg cccattgcat
   124861 aaccgcttgg ataagcttct ttcattaaga ctgcgcggtg atagactagt tccgctgcat
   124921 tctcaaatct caaagatcga caagcaacag ctgcacaaaa agcaccaatc gaatgtcctg
   124981 ctgcaatatc aggctgaata ccgttttttt ccatttcatc taaataaaat actgcttttg
   125041 tcagtaatgc tagctggata aaaaccgtat cttgatagtt ttcttcttta tcaactaacc
   125101 gataccccgt cacattttct aaacgttcca tgtacatttc agggatatcc gctaacatcc
   125161 ctaacttctg actgccttgt ccaggaaata agaaacctgt actcatgagc ctcgcctcct
   125221 cagcacttac tttttccact gctatcttag tcaatttctg ctataataaa aaatgccaaa
   125281 aaaagaaaaa accataacaa aaaggtatgg atgggatgga taattatgaa attgcttcat
   125341 ctgcagtatt ttaaaaaagt attcgaaaca aaaaatgtta cccaagcagc caaagaatta
   125401 tttatttctc aacctgcctt gagccgtgcg atcaaacatt tggaaaacga attaggtgtc
   125461 cctttgtttt accatagcgg cagaaacatt gagacgaccg tttatgcaga agaattttat
   125521 ccatatgcag tcaagacatt agacacatta gaagaaggaa tcaaattatt gtcttcggtc
   125581 aatgagaaga tcgttacttc cgtcatattg tatttagagg tagcctctgt ttctattccg
   125641 aacttggtcc gaatttttat ggaaaagcat cctgatatcc aattaacgat catgcaacat
   125701 catttaccag atgatattac cgatcctgta ctgtacatca cttcggagat gaagcctggc
   125761 atgaccaata ttcctatcat gaaagaacct gtttatgtcg ccattcctaa gaaacatcct
   125821 cttgctaaaa aagaacatct gcaattagaa gatatccaac gcacccccat cttgatgctg
   125881 tcaaagagca acgccttccg tcatacactg gatgcagcac ttgaagcgaa aagtatcgaa
   125941 ttgacgatca gttcgacgac agatgatcct gcgacattac gttctattgt caagcaagga
   126001 ttagctgtta gctttttccc aaaagtttct tggtcttacg ataaaaatga tcctttcgtc
   126061 ttgcgggaaa tcaaagattt gcccttgaca cgaacaattt atttatcttc tagcttcaca
   126121 gaagatcatc ctttgatcaa aatgattgca aaaacgatgc gggaatttta tttaggaaaa
   126181 taaaaaaaaa agagctggga caaaattctt gtcgcagctc ttttttcgaa caaacggtgt
   126241 tccagaagta atttcttaga aaataagccg aaatttccca aaaatttgag aagcaatttt
   126301 cgtaaattcc ttcttatttc ttgaagctaa acatttctgt cacaacctct tctctattgt
   126361 cctagacatt cattgcgttg atttaaaact tccatcaatc aaatctgcta gttcatctag
   126421 cttttctatt ttacgtagat caatagagat ccaatgttct ttattcatgt gataggctgg
   126481 aaaatacccc tcttttcctc gcaaaattcc gccaagttca ggatcgacct tcaaatcaag
   126541 aatattgatg atcctatggt cacttgcatc atcatagatt ttttcttctg gaacagacat
   126601 caggacccca aaccattttt tgcttttttg atgtctgaat acgcaataat caggaaattt
   126661 tttaaaaata tattctggag acaaattgta tgtttcattg atgtgatcga ttagttcttt
   126721 tttcgttagc atctgcttcc atctctttct tattgattcg ctttattcaa agataccacg
   126781 taagaataga aaaatgaata caagtagttg ctaatcaaat acttcgatat ttttcttttt
   126841 tatcaactct taaatgttca atccgcctgt gttggtcttg ctaccatcta ctattaaaat
   126901 gtttattatt gtttgcccac ctattttttg attttagtgg tatcattttt caagttcatg
   126961 atgaaattct tatcatcagt aaatgctcca gaaaaataaa caatgattga aaaatcagtt
   127021 gaaacttgac caccgtttat tttctccgta ttcttagaat agaagggagt ctgattgatg
   127081 tattcactaa tgaaaaaaat cattaccgaa aaagatattc gacgtcatgt ttctttagta
   127141 gaacaattgc tgaatcatac aaagataacc gtaaatgaat tagctgaaat catagggaca
   127201 accgaacgaa cgattttttc tgaccttcaa tctattcgct ctcagctgcc cgaaggctgg
   127261 gacatcatca gcgatcaagc aggaatcagt ttgcaaaacc agcaaaattt attaacaaat
   127321 gacctttggg agattttttt caaacaatca gtcagtgtcg aactgctgaa aaatctgctt
   127381 tttacaaaaa aagttgctgt ccctgatttt ctagccgacc atgggctttc ttatgggaca
   127441 ttgaaaagac atgtgactaa aatcaatcag cgtttagcaa gttacgactt gcagatcgat
   127501 ttgacgaaat atacagcttg tattcttgga aaagagcgtg cgattcgtac tttttatcat
   127561 cgattgctga ttcctttcac tcataacaac tattttttcg aggattattc gatccatgaa
   127621 tcccattatt ttcaattttt aagaaacctc agtcaaactg aactagcggt tgaaacagaa
   127681 gagattttcg gaacatgttg gttctttatc aatacgatcc gtataaaagc gaattgccga
   127741 ttagattctt ctatccatac caacagcaca ctctcctccc tgtatgactc tgcattgaaa
   127801 aaattgtatt taacggaagg tatttatctt aaagacactg agctttcttt tgcttctttc
   127861 tgctttctgg aaagttggaa ttacaacaat aactatggac aagaaaccgc tcgctgcctg
   127921 catcattctc catttttaga ggtcctcgaa acgtttgtag aggaactagc ttcagagctg
   127981 tctcttgatc aactaaaaaa aacatctctg actgataact tggctctcct catattaaaa
   128041 tatcatgaat cgccgatatt gattgaagca ttggaccgtc aataccatcg ttttttagaa
   128101 acatatgatc agcaatatgc acacttgtat caacagaaag agaatttact gaacgatcta
   128161 aaaaaagaaa tagaaatcaa tgaaccaaat tactttctcc aactcttgtc tctactgatc
   128221 caacaaacga ttttttctat caaaccgaat ttgttcaatg tctattttat tttccaagga
   128281 gaaccttcat ggaaggcgtt cttgcagcag gaattaaaag attacttagg aaaacgtgtc
   128341 tgcttcttac ctatcgaact aaccgatctt tccactattt cttttgaaaa aacagatatc
   128401 ttgatttcca atttccctct agattctcta gatatcccta ttgtatacat ttcttctatt
   128461 ccaacgaaaa atgagctcaa tcgtttgact gaactcactt tcaaaacttt tttatagtaa
   128521 cggctagcga ctgtattctc tagctgtttt ttcttttttg cgaaacagtt tgttcatgat
   128581 ccctttttct atttctacaa ctaagaatag aagaatcaca tttgctccaa tcaataactg
   128641 ttgtgtcatc gttaaactta ctgtaccgat cagctgctgg ccgactggta gaaaaataac
   128701 catcccttgc aataaagcaa ggatacctaa tgaaagaaaa agcgctttgt tttgcaaaag
   128761 accttggttc aatgacggct tggacaactc tctgcaattg atcatgtaaa cagcttgagc
   128821 taagacgata ttttgcagaa gaatggtttg ctgtaagctt tgcccttcaa actgcatagc
   128881 caataaatag gcaggaacca tgatcagtag ggagacatag atgatccgaa acaaatgata
   128941 accagatagg attccttgct ttggatctct tggtgggcgc ttcatgattt ctggatctgg
   129001 cgactcgaac cctagtgcat aagataaagt gattgtcgtt accatattga cccataaaat
   129061 ctgtactggg gacaaaggta gcgggcgatt cagcataagt gcccaaatca agatcaatcc
   129121 ttgagctaat gcagttggca agaagaaatt gatggttttt tgcaaattat ccaaaatccg
   129181 ccggccctct tttacggctt ttgcaatcgt atggaaatta tcatctccta aaaccatatc
   129241 agctgcctgt ttgcttactt cactaccttt gattcccata gcaattccta catcggatct
   129301 tttcaaagca ggtgcatcat ttacgccgtc tccggtcatt ccgacgattt ctccattttt
   129361 ctgcaaagct ttaacaattc gcagtttatg ttctggtgtc gttcgagcaa agacatcgac
   129421 tattggcact tgctgaatca actcttcatc agacataaga tccaaatcga tcccttctaa
   129481 aactttggca gtatgtttta agccaatttg ttccgcaatc gcttttgctg tgtctttgtg
   129541 atcccctgta atcattttca ctgagatccc tgcttgttgg cattccttta cagctttgac
   129601 tgcactttct tttggcggat cgatcaatcc ggcgatccct gcaaaagtta attgggttaa
   129661 agtctcatgc gtgagttcct gtttcgaatc tactttttta taagcaaatc ctaaaacacg
   129721 ctgacctttt ctcgcaaatt ctgcagctgt ctgactccat gcttcttgtt ctgcagaaga
   129781 aagagttgat ttagcaaata atacttctgg tgcacctttg acgtaaatca ccgctccttt
   129841 ttcctccgca tgcatcgttg ccatatattt gtattcagaa ctgaacggaa tctttgcttc
   129901 tatttttttc aaaggtagat tggctgtttc tgtgtgtcga atcagcgcaa cttctgtcgg
   129961 attgccactt aaattttctg ttttctgttc ttcttttgtt tttaattcct gacagttttt
   130021 cataatggac aacacgcagg cttcttcttt tgcagctaca tccacaactg tcatttcatt
   130081 ttttgtcaat gttcctgtct tatctgaaca gatcactgtc atagaaccta atgtttctac
   130141 cgaaggaagt cctttgatga tcgccttttc ttttgccatt tcatgtactc caacggatag
   130201 aatcatggtc aaaacagctg gcaatccttc gggtaccatt gacaccacca aagcaatcgt
   130261 agccgaaaga aggatatgcc aatccatacc atatcgaaat gtcgtaaaaa taactaagaa
   130321 taggctcaaa caaagaatgc cttgaaaaat ttgtttgttt aatcggttga tttttttaat
   130381 cagaggcgtc gtttgttgtt cgaccgattg caatgcttga ttgattttac cgatttctgt
   130441 cgtgtttcct gtttctacaa cgacaccgac tgcagaacca gattgtacaa gcgttcctga
   130501 aaaacccata tttttctggt cgccgatcga ttcttcttct gtcaaaacgg catgactttt
   130561 ttcagatgct tctgattctc ccgtcaaaat cgattcttcc actaataaat catgtgcttc
   130621 gattaatcgg atatctgctg gaacgacatc tccggcacgt aagacaatga catctccttt
   130681 taccagttct ttcgcaggga ttttttcttg catcccgttt gatagtacta tcgcttcttg
   130741 tcccatcagc tgcttcaaac cattcaatga ttcttccgct tttctttctt gccagtaact
   130801 aacgagtcca ttgacgatca ctacgaataa aataatactt ccttcaatat attcactaga
   130861 agcaaatttc aaaaaagagg caatgatcaa cacgatcatc agcagatcag taaaatgctt
   130921 tgctattttt ttccatcgtt ttgtctgctg ccttgctttt atttcattat atccttgatc
   130981 agtcaggcgt ttctgtcgtt cttcttgcga caagccttct gttgatgttt gatagttttt
   131041 caatacatct tctaccgtca atctgtacca gttcatccta tcccctcctg tttaattgat
   131101 actgttatta tacgcaggga agttttttca ggcgtatcga ttatttcagg ggggtctgaa
   131161 ataattcgtt ttgcaaaatg tatctgggcc taactgatat ttctattgct tattctttta
   131221 tatacaaaat ttaagaatac aaaccagata accaataaaa aaggaataaa gcgtgtcgtg
   131281 ctttcgaata acaagctgaa agcaattgaa gtaaaaaaca ccaacaaagc aagatcttta
   131341 tatctttgtg tagtgttccg tttcttttct ttaataaatt gaacatgtgc acacaagatc
   131401 gaagaccaca caaatagaaa acaactcgtt gtcatgcctg ccaaaagctg aaaaaacaat
   131461 cgactatcag ctacaaaaaa atgtagacaa agaccggcaa aaaataacat tcctgtaatt
   131521 aaaataccgt gtaaaggtac gcctttctta gaaagcgcac aaaaccaatg gggcaaactt
   131581 ttttcttttg ctgtttgata taaaatcctg ctagtactgt agactgcact atttgctgag
   131641 gacaaagaag caaccaaaat cattagattg acaattgctg ctccgccttg gcaatggatc
   131701 acttgcagca tagtcactaa aggactttct tttaaatcta aacaatgcca tggaacaaca
   131761 aataaaatcg acaaaatcgt tcctatatag aaaaagagaa tacgccaagg cagacagttg
   131821 attgcttttt tcaaattctt ttgcggatca cttgtctcgc caacggttaa gccgatcatt
   131881 tcaattccag caaaactgaa taaaaccata ggaaatgaga ctaccattcc agcaaagcca
   131941 aacggtagta attcatcgat ctgtccaaaa ttttctttga ttccgataga ctgggtatga
   132001 aaatctgaga cactcaatcc tatacccaat aagataaaaa ggaaaatccc caagatcttg
   132061 atgattgaga aaaaagattc caattcacca aaccacttga ctgcaacaag gttgactgcc
   132121 attaagatca ttagtgcgct acctccgctt atcaaagctg gtacatgtac aaaccagaaa
   132181 tggatataca atcctattgc tgttaattcc gcaagtccag ctccaagcca gcaaagccaa
   132241 taagtccaat tgacaaaaaa tgcccacttt ttgcctaaat aatagtcaac gaaatcactg
   132301 atagagttaa atgtactatt tgctaaaaat aaatcaccca tggcccgcat caaaccataa
   132361 atagccaaac cgcatatcaa ataaacgaga ataacagctg gccctgctga tgcaattgcc
   132421 tttgctgaac ctaaaaataa tcctgttcca attgcgccac ctaatgcgat taactggata
   132481 tttcgattag ttagagtccg tttcattgcc ggtttttcca tcaagttcac ctttttcttt
   132541 ttcaagcagc atggcgactt cctgttccca agaaggtttc tttatttttt caatacagag
   132601 tggtactaca aacaaaatga cactgatggc tgtgactgca attagattga tccatgttcc
   132661 agctttcatc gtcagtccga cagaaaatcc gattgccgca aataaaacaa taacgacagt
   132721 ccaaactcca agattgctct ttttctcttt ttttccaatt ctaaatggtc gattcaactt
   132781 tgcatgtgta taacgcaatc tcaaaactgc gatccccatg atgacataga cgatacagta
   132841 gataacagta gttgaattta cgagcatcaa aaatgcttga ttgacgccag gaatcagcaa
   132901 ataaaaaagt gcaaagattg agataatgac agactgagtc aaaataacgg atttggatac
   132961 gtcaaattga ttcgtcttcc aaaaattgaa ttttggtgga tacgcacctt ttctagcact
   133021 ttctgtcact gtctttgctg gtcctgaggc ccaagcagat aattggactg ctacaccaat
   133081 gaagacaagt aaactaaaca gattcacaat aagatgtggc aaaccaagaa tttggcaata
   133141 aatcgaaata gattgcgcga tattattcaa ttccatttgt ccttttggta cgacatttgc
   133201 tacaactaaa gcattcaaaa tattgacgaa aaatataaaa attaacgcgg caaatactcc
   133261 gcgaatatac gttttaactg gattttccaa tcgagtgata tacacagagg acatttcgat
   133321 accagtaaaa ataaacatga tcggcgcaaa ataaacaaat gaggatgcag tcgtagtatc
   133381 tggtattaat ttgtcccatg aaaaactgcc taatgttccg gttggtacga tcccaacttt
   133441 gatccacgca gcaaacccta ataagagcat catcgtcaaa ggcacgtaca atcctaacca
   133501 aacaccgact tttccgccga tttttgccat atcaaatttc aaattcaaaa aagtgatgat
   133561 ccaataaacg actaaaatga caactaatgt aaatttgctg ttcaaaccaa gcggcacatt
   133621 atcgataata ttaccaaaca acggagcgag tgctgaagcg accatcacca tcccaggaaa
   133681 catttggacc cataacagcc atgagacaac gaacccccat ttttttccta gagcattcga
   133741 tacccaaagt tctggaccac ctttttgggg aaacattcca gcaaattcac cagagatcaa
   133801 tgagattgga aaagcgtaca ataatactgc aaccagcatg taaaaaatca tcgtccagct
   133861 cgttgctgct acatctggaa tatttcgtac acttgctaca agggcgcagg tcattccgat
   133921 aaaggtcatg gtcgtgattt gctttgatga accagtatct ttcatcttta ttccttcttt
   133981 caatagcgac atatgttttt gttttctttt atttttgttc aagaacagaa atgacaaggt
   134041 ttcatcgatt cagtacaaaa cccagtatca ccgaaacatc cattgaacga tcttagtgaa
   134101 ctgggtttga tacatgaaac gaacgatcca acttatagaa cgttttataa aaaagaatat
   134161 cttggcgagg aatatatgaa aaacaggggg aaatacaatt gttttgatga agcaaataat
   134221 tgtctgttct ccatatgtct cttgttattc tttcacacaa aaatcagttc tattggatga
   134281 tttcgtttag tttcttttcc attgctttgg cgattttttt tgtataaaaa ttgaaaattc
   134341 gatcatcatt caaataagga gtcatgatag ccgcacgtag cagagtgatt ttctgttctt
   134401 ttttccattc ttctttcgtt agacccattc tttcaacaaa acgaatcgga gaatcaccgt
   134461 aatcttcttg tgtaaatgta gtatgagaag taataaaccg ttcaccataa acatcgccat
   134521 ccatataaga agaccgatcg aacatttttt catttaattc attgatagcg ttcaaatctg
   134581 tacagccttg ttctttgaat acccaattga ccatattaaa atcaggatga ttcaatggat
   134641 agacttcgat tgtttttcct ttaacagtaa aagtcagatg atcgagaaat tctctgaatc
   134701 tttgagctgc ctcgattgaa gccccaatca attgaccata tccggtcaca ttcaatggca
   134761 atacacgatg tgctgtccag actgcggcgg cggttgctcc agcttttgat ccttccaaaa
   134821 tataagctcc taacatatct ggagccttta ctgatttttc aaacacatat ggcgcgaaat
   134881 aagaaatgat atttctcata tttttatgtt tgattacgat tcctccagca gcataaggga
   134941 catagcccat cttatgagga tctatcgtga cagaatccgc ttctgaaata gctctgaacc
   135001 cttcatatac ctctttgtca atcgttacgc agtggtggaa aacatgatgt tcttcaaaga
   135061 attcagctaa ggaagcatac ggcacaaatt ctcctgcttc gtttagaaac agtgaacgcg
   135121 catagccgcc atatgcggca tcaacatgta aatagaaata gatcccttca tcttttaacc
   135181 tttctcttaa ttggacgatt ttatctacac tgtctacttg accttcttct gtcgttccta
   135241 cgacagcaac cacacctaga attgggattt tttgtccagc taattcacga attgtttttt
   135301 ccaaagcatt aatatccatt cgataatcct cttgaactgg gatagcaacc atctgatcta
   135361 agccgactcc gcaaatatct aacgccttca tccaagaata gtgttttgtt tgagggacca
   135421 gccatttccc taatctttgg atattttttc cgctacggga agaagctgct ttgacctcat
   135481 ccatttcttc gtccgtaaag ctttcggtca tttccaaaat ttcttcaaca gataaattca
   135541 aaagttccca ctcagacatt tttttcactt tttcaggcag cacttccttg acagctaaag
   135601 gaatcgattt gatgcaacgg gcgtaccata atccttcaag attagcaata gaaccatctg
   135661 ctgcaatgtg tccccatccg tctgtcatat tgaataatga agcaaaatcc tgtcctactt
   135721 ctgcttccat ttgcgaagtt gccatagaag attccaatgc aacattattt gggttccaca
   135781 gcatcgcata gttgtaagct aataaagcag gcatcaatgt ttcagcattc atttgtcccc
   135841 agtaacggcc tgctgaatgc catgggatcg atcctgcgcg taatcgctga ctgacttcat
   135901 ccaagacctc tatcatatgg tcgcgggtgg ctagataatc tggagtgagc ttatctcctt
   135961 cgctaattgc aggcatatct gaaggaatat aattttctct ccatccaaga tgttcatcta
   136021 ccatcttgtt caacaacatt ttataaactg gtccattttc agctttgtcg cctataaaga
   136081 cggccttgat atccatatct ttcatttttc ttccctccat attttgttgg tcaatgtctt
   136141 gcagatacat gtgtttatct gtaacacctc ttcactatag cgccaaaaat gagaattttt
   136201 tttttataac atcaaatcca cgtagatttt tacaaacaaa aataaataaa gtcggatttt
   136261 tctgataatt caacgcaaaa aagcgaacaa tttttcaata aatcagtctt ctaaaataga
   136321 taacctaaaa aaagcgaaca aacaaataaa atgactgtcc ttcattcact aaacatattt
   136381 ttattttttt atattgttaa aaattcgcaa tttatttttt aatagaatta tctgttttcc
   136441 aaattttata acataaaaaa ccttttttaa cggcatttat atgctttttc agaaattaac
   136501 tgatgatttc aaaaagaaaa catgtcataa tatttcccat attgtgcaaa aaaatacaag
   136561 gcggaggctc tactatgatt gaagaatata ttgagaaaaa tattttaaga cagctgtttt
   136621 tatgtgggca attctatgta aataaagaag tcaatctaga aaagctctcc aatctcctgc
   136681 atgtttgcaa aaccacttta ctcaatgata tcaataacat aaaaaaagaa tttgaagaac
   136741 agatcgctta tacacatcgg gaaaaggacc gttacacttt atatttttca gaacatatac
   136801 ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt gtttttaaaa acttgtctgc
   136861 tttatttgga agaagatgaa cctgattatc ttcaattaac tgaatgtgaa ttcatctctg
   136921 tcagcaaagc ttattcatta aaaaaacaag tattagctta ttttaatgac tgcgggatcg
   136981 aaatcgaccg ctactcccct cgattcactg aaatggaaag acgtctgctg cttcttaatg
   137041 tgtcgtatcg tttaggcggt tttaattctt gggagctacc tgaatcgttt tttgaacgtg
   137101 ccgatcgttt cattgaatcc gtaacggaaa atagtggcag attttatgat aaagaaaata
   137161 aagaaatctt atcgattggt tttgctatca gtttcctacg ccagcaagtg tgtgctgtga
   137221 caatagattc aaaatttatc gaagaaatca aaaaacgtcc tatttacaat tatgtcgaat
   137281 cagcatggga aaacactgat ttccaaacct attataaaaa agaagaattc gcctttattt
   137341 tgacactatt caatctttgt aattatggtt tccattctta tcagttgatt gcggaagact
   137401 ttcaacagct acaccaagtg tttattgaca atactcccga aataaaagaa ctagttgcta
   137461 cctttgaatc tcacttcaat caagaattat tcggaaacca accgttcgaa cgtgcgctga
   137521 ttcatctgat gcgttcggct tgggataact atcaattatt catgccagaa aaattttatc
   137581 ttttaaatga ggaacagaca aatttatata aagaagtcca gacaattttt tccagctggt
   137641 ccagccagtt accttatgat ctacggttga atcctaattg catgcgggct tttgtgatcg
   137701 aattgagcgg aattctccgt ctgacaaaag aacatctgac tatctatatc gtcacaaatt
   137761 cggatgttca ttatctgatt tatcgggaag ccttggaagc tgtaactact ttcgattttc
   137821 aagttgcacc aacaatttat tcaagtatat ctgatataaa gaagtatgcg cagcagtcct
   137881 caaaccgggt attatgtgaa cgtacattat atacaccaga tgcggtccaa tatgaaaata
   137941 ttattcctat ttcgatcaat acgatcgatc gagctatcat ttcagctgtg caaaacaaat
   138001 aaggggctgt gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc ccatttctgt
   138061 aacaatctct tgatacacgt caggctcaca acctcactta ttttggacat ctacgggtcc
   138121 gatctcttta tttaaaaaat aagaaatgat ggttcggata actaccaacg ccgctagttg
   138181 gaaaatatcc tgcaaggtag gtttgataat cgattcgatg atcccagctg aaacaagaat
   138241 ttccaaactt agaagtatat agcctcccaa catcttttta atagcattgt tctcgctgac
   138301 tcggtcgctt tgcacttttg tatggacaaa taaatatttc aaaaaatcct tgattgatag
   138361 acaaacaccc caaaccaata aacagatagc aaagatatca agtcccagta caagtttttc
   138421 gaaatatgga agaagaaact gcatcaactc tgtcgtcatc ctacctctcc tcttctttgt
   138481 ttttctttgt cttctatttc ctgattcaag aaataagaaa tgactgttcg aatgataata
   138541 ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga taactgactc gatgatatca
   138601 gcaacgacta gtacttccaa gcttaacaaa atataacttg ctagcagttt tcttgtatct
   138661 gcattacgag tattacgttc tgcatagttt ttcttgacat gagaccaatc aaaaagtttg
   138721 aatagaacaa gaacgatccc gacaattaag ataccgatag aacaaatatt taaaatagca
   138781 atgatccatt ctagaaattc aattaaattt gagcgaaaca tatacgctac ctactttctt
   138841 ttaatttgtt cctattcttt tattgtaaaa gaaagttacc ttgttcgtct atttaaaagt
   138901 tgttctaatg ataacggttc gcttaaattt ttctttttca ctctcccatt tttctccctc
   138961 catccataaa tcgtggaagt atccaaaaca acgtagcaag ttaaaataag gcttagtccg
   139021 tcatcagctt ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa tttctaaaaa
   139081 tagaaccata cacctcctca aatcttttct tcttacctac cctattttta tttacaggaa
   139141 aatcagacac ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct atcatttggc
   139201 attctaataa aaacgaacta tccttggata tagaaagaga aaacaaggag gcgtttttca
   139261 tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt gcttgccctt ggcgctgcat
   139321 atctaggaaa aaagacagga ctattcgaag atgacagtca tttatatgat gaattcgaat
   139381 caatctagca tcctatttag ttgaatcact acggacatac ttggaagaca ttggaggaat
   139441 cacaatgaga aaaagtaaag caataatgat tggtgccggt attgccaata tggcagcggc
   139501 tgtttatttg attcaagaag gcaaatggaa aggggatcag atcacctttt attcattgga
   139561 tgatcacggt tcaaatgatg gtgcaccgac cgacactgta gtggattaat attggaataa
   139621 aaaccatcca ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca tgcttaatta
   139681 ccgtacctat gtagatttga tggatctgtt aagtcgaata ccttctgtta ctgagccagg
   139741 gatgactgct gaagaagata cgagagattt tgatgcaaaa caccgaacct ttgacaaagc
   139801 acgtttgatg gaaggtggca aaggaatcat tgatggcgga aaactgggat tcaataataa
   139861 agaccgtatt cttctgacta aactgattgc tatgcctgat tcagaagaag aaaaactaga
   139921 caatatcact atcgaagaat atttcaaaga tgatccacac ttttttgaaa caaacttctg
   139981 gtttatgtgg gaaacaacat ttgctttcag aacacgtagt tctgctcaag aattgcgtcg
   140041 ctatatgcat caaatgattt atgaattcac tcaaatcgaa catctagtgg gcgtcaatcg
   140101 aacacgttac aatcaatacg aaagtattat gcttcctttg atcgagtact tgaaagaaca
   140161 aggatgcaaa atcattttga accgccgtgt cactgactgg gaattcaaag aaacaccgat
   140221 gcaagatgaa atcactgtga ctggtttgaa aattaccaat acactgactc aagaagaaga
   140281 atacgttgtg gtcgatgaag atacagcagt tattttcact aatgggtcca ttacagattc
   140341 agctacttta ggagattacg aaacaccagc tgtagaaaat atggattatg gtgcagcttc
   140401 tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg acaaattctt
   140461 tgctgatcga gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa accatttgtt
   140521 gttgaatgaa atcacccgta tcacgactca agaaccagga aatgccttga attcatttat
   140581 ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta tcgtcgtgca
   140641 tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca gttatttggg gttatttcct
   140701 ttatccaaga cgacgtggcg aatttgtgga taaacagtat attaaaatga atggaaaaga
   140761 aatgctagaa gaactgattg gacaactttc aaaagtagat cctggaccag tgaatattcg
   140821 tgaaaaagaa acagaaattt tcgacagcgt aataaataat atccctgtct atatgcctta
   140881 tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca ttcctaaaca
   140941 ttcgaccaat ctggccttta caggagaatt tgtcgagcag ccttatcaga tgatctttac
   141001 tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc cattttgcgg gtattcctat
   141061 gtcccgtctt gtcaaaacac cgcgttacga caaagatatt cctactctag ctcgtgccgc
   141121 aaaaaaaatg tttgaataat caagaggttg tgaaaaagct gccctgcatc aagtaataag
   141181 aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg ttggacttat taccgcagat
   141241 gcaagctttt gaacaccgtt taccaagagg ttgtgaaaaa gctgccctgc atcaagtaat
   141301 aagaacaccc aagctaaaac tgcttcttat gtttttgact tggttggact tattaccgca
   141361 gatgcaagct tttgaacacc atttaccaag aggttatgaa aaagctgccc tgcatcaagt
   141421 aataagagca gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc
   141481 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg
   141541 agtatgaaga aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat
   141601 accgagaaac tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa
   141661 aacaactatt ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac
   141721 tatttcgatg attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac
   141781 ataagactat agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat
   141841 ctcctttaaa atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa
   141901 ggagcatctc caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac
   141961 gtagatgatc caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc
   142021 atttgcggct ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat
   142081 gttgtaggac acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg
   142141 aaaaaagggc aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc
   142201 ctgaatcaca tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt
   142261 ttcggatttg gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt
   142321 ccttatgcag actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt
   142381 ttatttctat cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa
   142441 aaaggcgata cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc
   142501 gctgttatgg ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat
   142561 aaagcggcta attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga
   142621 acagaattgt atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg
   142681 gacggattag agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca
   142741 atctctccat tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact
   142801 ggtgtttata tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc
   142861 gctgtcaagc atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt
   142921 gcagaaggac tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca
   142981 gcacaagcat atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa
   143041 ccttgaattc agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc
   143101 catgagatcg aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc
   143161 tatttccaaa atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc
   143221 tatatttttg gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt
   143281 tatttggctg gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt
   143341 tttatttacg acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa
   143401 tttttcaaat ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg
   143461 tcgtcccact ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg
   143521 tggtatgatc tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga
   143581 tttccgctaa tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg
   143641 cacaaagatt tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc
   143701 ctccgacgat ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat
   143761 tagcgattcc agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg
   143821 aaaaccaatt cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat
   143881 atgtaaccaa tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct
   143941 ttatgaaaat tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact
   144001 tgttctccgt tttttgattg atcaagcatc taaagcttct tttcttcttt cttggatcgt
   144061 agctaccttg acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag
   144121 tacaccacct gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca
   144181 tggaggcata acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact
   144241 aactaagacc attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg
   144301 taacccaaac atcgttggtt cattaatatt gaaggacacc tggcgcaatc gacaagtttg
   144361 ttacaaccat atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta
   144421 aagttgcacc tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa
   144481 acggtgcgct atgcccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga
   144541 ttgtttgcat caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg
   144601 gtgtgataaa tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt
   144661 cttggaccac tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg
   144721 cagcaactaa agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt
   144781 ttgcaactgc cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta
   144841 aacgtacaaa gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg
   144901 agctcatacc taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg
   144961 aaacaccgtc ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga
   145021 cagaaagaat ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac
   145081 caccaaaaat tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta
   145141 ctgctaatgc tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa
   145201 ctaaatgccg ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg
   145261 caacaaattt atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga
   145321 cgatacagat caatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga
   145381 tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc
   145441 agcatctctg tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt
   145501 tcatctgcta aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt
   145561 tttgcatttc cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc
   145621 atttattgta tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag
   145681 tactttttca ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc
   145741 tttaggtgct agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag
   145801 taaaacgtct acatttttag attctagttg attatccgca tctgaagcag atacagcaaa
   145861 gatgtctgct tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact
   145921 tgtactcatt ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt
   145981 cctttctata cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa
   146041 aaagttgata tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag
   146101 cgcacactaa acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa
   146161 agttgtgaca caaaacttgt cacaactttt ttcaaataaa cggtattcaa aagcttgcat
   146221 ctgcggtaat aagtccaacc aagttaaaaa cataagatgt tgtttttact ttgctattct
   146281 tattacttga tgcaggacag ctttttcaca acctctcaat aaacggtgtt caatcagctg
   146341 accctgggta ttagctcaaa acgctggatt cacaacctct tttatttcaa ataaccagta
   146401 aatacaatga acatctacaa aaaacttcta tttatttata gatttcgtca ggcaaatcca
   146461 attctctctt atcaatcaaa gtctgatacc acgaagcaga ttttttcaat gttctgcttc
   146521 gttcatcgtt caattcgatt cgaactaatc catatcgatt cttaaatgca ttcattggtg
   146581 acacacagtc agtgaatgcc cataacatat agccttcgca atttgcgcct tcttctgttg
   146641 ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat acggtaatca tcttgcacga
   146701 ttcctttctc atccatatac tgttcttcat attctcttcc cataccgttt tctgttatca
   146761 gccaagggat attatgataa ttttctttta gatacatggc tatatcatac atgattttcg
   146821 gatagatttc ccatcccctg gaattattca ttttcttccc aggtaaatca aagacttcat
   146881 aatatcgttc aggatggaaa ggtgtctctt cattccattg ataacgaggg gcgcgaacgc
   146941 gtttaggata atattgattg attccaagga aatctactgt atgatgctga atcgtttcta
   147001 aatcctctgt tgcaggatcg aaataaatcg attgtttttg acacaactcc aggaactctt
   147061 ctgtgtagta tccttttacc atcggatcaa aaaatactcg attataaaac aaatcataca
   147121 tataagcagc tttttggtct tctggagaat tggatcgagc atagaccatt tcaggattta
   147181 aaatacagcc aatccttcca ccataatttc cgctgcggta aatctctact gctttagccg
   147241 ttgctaaaac tttatgataa ttccacatca tccacttttt tgtgttttgt tcatgcggcc
   147301 agcgaacagc atccaaatag caacgcgttt ggggaacgat tggttcgttg aatgtaaacc
   147361 agttttttac cctatcaccg taacgatcaa aggcaatttg agcatactta gcataaaaat
   147421 caactacttt tcttgaagac cagccatcat atttttccaa aagaaaagct gggacttcat
   147481 aatgttccaa acaaatcatc ggttctacgt ctgcttcgat caaagagtcg atcatttcac
   147541 ttatatgctg agcatattct tcatctacta tacagttctc ataatcagta aaaaaacgag
   147601 cccaattgat agaagtccga taatgtgtca gatggatgga tttcatcaga tccacatcct
   147661 gctggtattg ttccataaaa ttagttgcta cggctgggcc atacccatta tgccaaacaa
   147721 aatgttcatt tttataccag ctatctataa acgaatcctg tccttctttt tttcctttcc
   147781 aaccttccgt ttgccaagca gaagatgcag caccaagaat aaaatttttt ggaatagtaa
   147841 ttttcatgat ttcctcctgt tttttagatc ccatcatttt ccacacgatt tgctactttt
   147901 acaaatggca gatagatcaa aatagaaaca ataatacaaa caagttgcgt aataactgca
   147961 ccgatatttc ctgctgttgc cagataagca ttgacgatcg gcggcattgg ccaagggacc
   148021 attacaactg ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat cgttccagtg
   148081 ataagaggcg ttaagataaa cggaatagct aaaattggat tcagcatgat cggcatacca
   148141 aaaataactg gttcattgat gttgaagatc ccaggagcaa gggataactt ggtgatttgt
   148201 ttcatatctt cccgttttcc aactaacaat actgcaatca gtaaaccgat cgtgactcca
   148261 gacccgccca tactcatata catatcccaa aatggcatag taataatgtt cggtacttct
   148321 tttccagctt caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt
   148381 atcggcttca ccatttgatt tccatggata ccaatcaccc aaaagacttg cgcaataaac
   148441 atcaatagca aaatacccgg taacccttgt acgacacctt ctaatgggcg ttgtacgaca
   148501 ttgtaaatga tatcataaag gtacattcct gtaagttctt tgatgataaa tcccgcagcc
   148561 gcgatacccg atacagtcag aatcgtcggg aataaagcag aaaaactagc agaaacattc
   148621 gttggcacgg tatcaggcat tctcagctgt agtttcttct gtttcctcaa ccagcaataa
   148681 agttcaatag aaaggatgga gaccaacatt cccaaaaata accctttcgt atctgtatat
   148741 tgtttagcca atacgttttc tactataaca gtctgatttt cattcactaa ttcataaaca
   148801 gttggattaa cagataaata agacatcact gcgatgatac cagggaatac accatccact
   148861 ttattcagct tggctaattc gatccctatc agaaaaacag catagatggt caagaaactt
   148921 agtgtgacat agctgatggc agatgcgata ggctttaatt cttctaaaaa acgcaagaaa
   148981 cttatctgtg ctaagccatt ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa
   149041 gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt tttttatcac catgatatac
   149101 cttaaattat tgattctcgt tgccacgttc cctaaaacat tggctaattt atccataaaa
   149161 ctcattccag atcccctcat tcctgcttct tctccttgac gtcatctctt tttgtctatt
   149221 agtcagcgct tacatttcga tgaacagaaa ctatttactc ctctttttta ccaaatataa
   149281 aacaaaaatt catctgatgt atttatcata aaatacatca gatgaactgt caattagttt
   149341 ctttttctcc tattattttc tgtaattctc tccgatttat tgccatatgg acagtcattg
   149401 catcaaacgc agcaagtgca ttccgccttt cgatggcctg aacgatctct gtatgaatat
   149461 cgatcgtctc gattttcaga tgctggatat cataattttt attaaacaaa gcaattgatt
   149521 cattaatgat tgggataata tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg
   149581 tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa
   149641 tcttttgcgt taattttttc atttggacga tttgattctt cgttgcatat tccgctgcca
   149701 aacctgccat cttaggctcc aataaatatc tcagttcaaa taagtctcgt atcatccgat
   149761 cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc aatccctcgc ttatcactga
   149821 caaatgtccc cgctccttgc cgaacttcaa ggatattcct ggaagctaga gcccgcacag
   149881 cttctcgcac cgtgcttcgc cccacctcta aataatcgga caattcatat tcattcggca
   149941 gtttatctcc tacttgcaac tgcatttcct ttaaatatct aaggatcttg tcagtagtta
   150001 cttctactag ggttttattt tcttccaaac cacatacctc cttttcatct gatgtatttt
   150061 ttcgtaatta ttataacacg tttgaatcat ctgatgattt tttgtataca aaattgatta
   150121 atccgttcat agacatacaa acgaagaaga tatttaccat aaaaaagact aggataaaat
   150181 tcactcctag ccttcgatca tttttatttt gaacgacaaa aaatatgatg ttcaataaca
   150241 gacgatcttc attcgattct tgatgtcatc ttttgttttt tttaatatat ggataaaaag
   150301 tttgattaca tcgtttctat tctagaattt ggtcgattct caattcttct tctggtgaga
   150361 aatctaaatt atccatcatt cgaacacttt cttgtaactg acttaaccga cttgctccga
   150421 ccagtacact tgtaacagat ttttgtcgca aattccatgc aagggccatt tgcgctaatg
   150481 attgtcctcg tgatacggcg attgtttgca gtgctttgat tttttccaga gttgatccaa
   150541 cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc tctagaatct tcaggaatac
   150601 catgtagata acgatttgtc agcaaccctt gagcaagcgg actgaacgtg attgccccta
   150661 gcccttcttc ttctaatact ggagatagat cgtcttctag tagtgcacga gaaaacatat
   150721 tgtagcgcat ctgatgaata atgaacggtg cttcctttct ttttaaaatt tcagtcattt
   150781 tttttgtatc ttctccatta tagttggata taccaatata taatgctttt ccttgtctta
   150841 caagttgcat caaagcttct gctgtttctt ccaaaggtgt atcagggtct gggcgatgat
   150901 gatagaagat gtcaacataa tctagcccca ttctttggag actttggtca caacttgaaa
   150961 tgatggattt acgagagccc cattccccat atggcccttc ccacatatga taacctgctt
   151021 tgctagaaat aatcagttca tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa
   151081 agtttttctc tgcagctcca gcagggcctc cataattatt cgcaaggtcg aaatgggtaa
   151141 ttcccatgtc gaatgcgcct cttaaaatct ctctttgatt atggatagga tcataatcac
   151201 caaaattttg ccataatccg agagataaaa tcggtaattt caaacctgaa tgaccggatt
   151261 tacgatattc cattttttca taacgtgcac tatctgcaat atacaatttt tctcctcctc
   151321 atcaaatctt tttaaaacgc tttctttttt ctatttttag tttactacat tttaaagaaa
   151381 aggagttaat caaaatgatt ttttcgtata tttttttgcc atttgttttt tactcgttga
   151441 tagatcagaa attgattacg tgcttgctgg atttttttat aacgtttcgg ttgttttttg
   151501 taaaattgct gatgatagtt ttctgctggc caaaacgtac cggctggttc gatttttgtg
   151561 acgattggtt ggtggaaagt tcctgaactg tctagtttct gttttgattg ttccgctaat
   151621 tctttttgtc gttcgccttg ataaaaaatc actggacgat attgcttccc tctatcttga
   151681 aattgtccaa atgtatctgt gggatcagta atttgccaat agattgtcag caactcgcta
   151741 taacttattt ctcttgtgtc ataaatgatc tccacagctt ccacatgacc tgtgtaacca
   151801 ccacttactt gatcataatt tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca
   151861 gataggatcc cttttttcga ttcaaacggc tcgaccatac accagaaaca tccccctgca
   151921 aatactgcac gttcttgata gtcagcatct tgcatggcat gcttggaaaa gtcatatttt
   151981 tgctccttat caaatccttc cgtaatcaat aaataaaagt ccattacatc aggcgtcaga
   152041 ttcattcgga tagctaatgg acgtagtgtt gcttctagtt ttgataactg ttctttaacg
   152101 gatatatttt ctcttatgtc attctttgtc tgaatcaata cttttcgttc ccaatctcgt
   152161 gtattcggat tcaaaatcag attataaagt gtttgaatga atatttgttc ctcatccata
   152221 gattctcctc ctttccgaag aagacaagct agtgacttta gctgtaattt atcataaagt
   152281 gaggaaataa gcgattactt catcccccta cagatttttc gtataaaaaa gagagaatcc
   152341 ttatcgacag attctccttt ttaatcaatt tattttataa atgaaaagtt aatttacctt
   152401 ttatggaaaa ctattttaaa aaataaacat aaatatgcag ttaatgtaac aagataagaa
   152461 ataatagtcg ttactgtata acttgtgctt actttaatat cagcaactcc tttgactccg
   152521 cttatttgta ccaatccatt agcattcacc tgctccttta caggttgatg atttatttct
   152581 acattcgttc ctaaataagc atatacagga agtgtgatta cttggctctt ctcattagtt
   152641 actctatata tgatagaact accagttgtt ttataaggaa ggtttaccca ttgactgcca
   152701 atctttgctt cgtgttttat tatatgctcc ttattctcca atgaagcgat aggagaataa
   152761 tcatataaaa aataatccat tcctttagtg gtatacgcct ctactgtttc tttgtttaca
   152821 acaatatgtc ccggctgaga aattattttt tgattgaaat tttttttact agaaacagtt
   152881 aaagtgatta ataacaaaac agtcataaat aaataaaata gttctcttgt cttattgtat
   152941 cttctatttt tgaaaaatat catcgttaat atcagagaag agaatattat ttgcaagctt
   153001 aagatccgcc aaggaaattg taacaatttg gctggcgtat tttgcagaag ataccatggc
   153061 accaaactag ttgtagctag ccaagtcaaa cacgtaccat aataaatctt tttttctatg
   153121 tttgttaatt ttttaaataa aatcagggga atacataaag ataacagacc aactagtccc
   153181 attgagtaag tagttaagtc gttgttcata gcttcagtca gactatcgaa cacattagat
   153241 gcatgagcat ataaatttgt cacgctaggt ttgttgattt tttggtagag catttgttca
   153301 aacatcggat accaaaaagc actcgtcaga accaatgtca ttcctgccgc actaaataat
   153361 tttttaaata tagcaataaa atctgcattc ttgaaacctt tagaaaccaa acgaataaaa
   153421 atgataatta atgctgtact cattaatgtt gatagtacat ggctgtacac tagcaaactc
   153481 atactcaatg ctagctttac agacggatac ttttcttctt ttccagagat gatttgataa
   153541 gcataaaata atacagggat aacaagtgtt attgcgatag cttcggcaat tgcggaacga
   153601 tagtagatgt ctactgtccg ataagcagag aaaatataga gacaactaaa taaaaatgcc
   153661 gctttttttt gttttaaaaa ccgctcgcca taatgataag ctacgagtgt tgtacacaag
   153721 ttcaataacc aaacgtaaat aatatagctt acaatcaaat tttttgtcat ccaatataac
   153781 attacagctg gaaaaaaagt cagaaaggga taaaaataat ttacaccata tccagtataa
   153841 ttgaatgtct cataattaat cggactggta aaaatagtcg ataacccctt gattcgagat
   153901 aagtgaaaag aaagatctcc agtggtataa atactctgtg tatttttgaa aaagatgaaa
   153961 gaatatatac tactaactat aattaatcca ctcaatatta ctactttttt atttattttc
   154021 attcttcttg tttatctcct ttttagtcac atatcgagaa tctttcaact tattaggtgc
   154081 aaatcgttta aaacttgaat acaccactaa acaataagtc agaatactga tccataaagc
   154141 catgagagca gttggagtat attttgttgt aatttctatc ttattttctc cagcatttac
   154201 atccacattc actaagccat gttttttata agagtcattt acgactttac cgtttacttt
   154261 tacctgtgta cctaaataag catatactgg tactgtcact ttttgctttt tgttgcttgc
   154321 cactgtgtaa gtaatgttat tatctgcagc tttatacttc cctttttccc acgattctcc
   154381 tacttttact tcatgttttt ctaagtgagg cctttctttt atcgcttctg ttggggcgta
   154441 atcgtataat cctcccatat ctgatgttgt ataacgtggg agtgtttctt cattgataac
   154501 aatatgaccg tgttttgatt ggataacttt tgcgtaattt tcttttgctg ctatagacgt
   154561 gacgagtaga aacaccacag tagcgccaat gattaaccat gaatattttt tattcatagt
   154621 ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga tacctaagat
   154681 acgccaagga aactggagca agcttatcgg tgttttttga aacaaaaacc atggaaataa
   154741 attagttacg catacccatg tagaaactac gccaacatag atgatttttt cttttttctc
   154801 catttctttc agtatgaata aaggaaaaat caacgcaacc aatcctaata accccatagt
   154861 gtatgttgta atatcgttat ttaacgcttg aatgatacta tctgaaatag aaagtgcttc
   154921 tagctgcaaa tctgttttga agggactgtg tatttcttga taattcatct gctgcaacat
   154981 aggataccaa aaatagctag ttaatgcaca agtcaaaaaa acagccttta ctaaattcac
   155041 gaccacaaat tttatatcgt tttttatttg cttgcttaaa aacaaacgaa ctataatcat
   155101 acaagcaatc aatgcactac acaatagtgt agttaagaca tgagtataca caagtagact
   155161 tatactgaca gataaagcga tgactttttt ttctctattg aatatcaatc cataagcata
   155221 atataaaaca gctgggataa tcgtaagagc gattgcttct gcaattgcgg atcgataata
   155281 gatatcaact gttcgataag ttgcaaaagt atataaacaa ctaaataaaa aagcagcatc
   155341 tatcttttta aaaaactttt ctccataata aaaac
//
LOCUS       DAFBQW010000003       106752 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFBQW010000003 DAFBQW010000000
VERSION     DAFBQW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 106752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.003"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(717..3866)
                     /locus_tag="LK889_000298"
     CDS             complement(717..3866)
                     /locus_tag="LK889_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBK5094563.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5124)
                     /locus_tag="LK889_000299"
     CDS             complement(3880..5124)
                     /locus_tag="LK889_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK5094564.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5117..6709)
                     /locus_tag="LK889_000300"
     CDS             complement(5117..6709)
                     /locus_tag="LK889_000300"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK5094565.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(7218..7943)
                     /locus_tag="LK889_000301"
     CDS             complement(7218..7943)
                     /locus_tag="LK889_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094566.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(8097..8423)
                     /locus_tag="LK889_000302"
     CDS             complement(8097..8423)
                     /locus_tag="LK889_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094567.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8460..8825)
                     /locus_tag="LK889_000303"
     CDS             complement(8460..8825)
                     /locus_tag="LK889_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK5094568.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8862..9077)
                     /locus_tag="LK889_000304"
     CDS             complement(8862..9077)
                     /locus_tag="LK889_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5094569.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9206..9541
                     /locus_tag="LK889_000305"
     CDS             9206..9541
                     /locus_tag="LK889_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5094570.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9617..11614)
                     /locus_tag="LK889_000306"
     CDS             complement(9617..11614)
                     /locus_tag="LK889_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094571.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11712..11987)
                     /locus_tag="LK889_000307"
     CDS             complement(11712..11987)
                     /locus_tag="LK889_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094572.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            12219..12953
                     /locus_tag="LK889_000308"
     CDS             12219..12953
                     /locus_tag="LK889_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5094573.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12992..14134
                     /locus_tag="LK889_000309"
     CDS             12992..14134
                     /locus_tag="LK889_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5094574.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14229..15620)
                     /gene="sufB"
                     /locus_tag="LK889_000310"
     CDS             complement(14229..15620)
                     /gene="sufB"
                     /locus_tag="LK889_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK5094575.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15625..16095)
                     /locus_tag="LK889_000311"
     CDS             complement(15625..16095)
                     /locus_tag="LK889_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBK5094576.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(16082..17317)
                     /locus_tag="LK889_000312"
     CDS             complement(16082..17317)
                     /locus_tag="LK889_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5094577.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(17314..18600)
                     /gene="sufD"
                     /locus_tag="LK889_000313"
     CDS             complement(17314..18600)
                     /gene="sufD"
                     /locus_tag="LK889_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK5094578.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18613..19386)
                     /gene="sufC"
                     /locus_tag="LK889_000314"
     CDS             complement(18613..19386)
                     /gene="sufC"
                     /locus_tag="LK889_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK5094579.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19583..20431)
                     /locus_tag="LK889_000315"
     CDS             complement(19583..20431)
                     /locus_tag="LK889_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK5094580.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(20455..21141)
                     /locus_tag="LK889_000316"
     CDS             complement(20455..21141)
                     /locus_tag="LK889_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094581.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(21134..22168)
                     /locus_tag="LK889_000317"
     CDS             complement(21134..22168)
                     /locus_tag="LK889_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5094582.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(22231..22487)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22563..22901)
                     /locus_tag="LK889_000318"
     CDS             complement(22563..22901)
                     /locus_tag="LK889_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBK5094583.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22903..23259)
                     /locus_tag="LK889_000319"
     CDS             complement(22903..23259)
                     /locus_tag="LK889_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBK5094584.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(23265..24452)
                     /locus_tag="LK889_000320"
     CDS             complement(23265..24452)
                     /locus_tag="LK889_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5094585.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24643..25488)
                     /locus_tag="LK889_000321"
     CDS             complement(24643..25488)
                     /locus_tag="LK889_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5094586.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25722..26687
                     /locus_tag="LK889_000322"
     CDS             25722..26687
                     /locus_tag="LK889_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBK5094587.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            27064..28107
                     /locus_tag="LK889_000323"
     CDS             27064..28107
                     /locus_tag="LK889_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBK5094588.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(28169..28633)
                     /locus_tag="LK889_000324"
     CDS             complement(28169..28633)
                     /locus_tag="LK889_000324"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK5094589.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            28879..29826
                     /locus_tag="LK889_000325"
     CDS             28879..29826
                     /locus_tag="LK889_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5094590.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(30037..32109)
                     /locus_tag="LK889_000326"
     CDS             complement(30037..32109)
                     /locus_tag="LK889_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5094591.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(32042..32419)
                     /locus_tag="LK889_000327"
     CDS             complement(32042..32419)
                     /locus_tag="LK889_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBK5094592.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(32548..33126)
                     /locus_tag="LK889_000328"
     CDS             complement(32548..33126)
                     /locus_tag="LK889_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5094593.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            33258..34550
                     /locus_tag="LK889_000329"
     CDS             33258..34550
                     /locus_tag="LK889_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5094594.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(34610..35728)
                     /locus_tag="LK889_000330"
     CDS             complement(34610..35728)
                     /locus_tag="LK889_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBK5094595.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(35742..37163)
                     /gene="arcD"
                     /locus_tag="LK889_000331"
     CDS             complement(35742..37163)
                     /gene="arcD"
                     /locus_tag="LK889_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBK5094596.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(37409..38530)
                     /gene="mnmA"
                     /locus_tag="LK889_000332"
     CDS             complement(37409..38530)
                     /gene="mnmA"
                     /locus_tag="LK889_000332"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK5094597.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            38934..39533
                     /locus_tag="LK889_000333"
     CDS             38934..39533
                     /locus_tag="LK889_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5094598.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(39671..40957)
                     /gene="efmA"
                     /locus_tag="LK889_000334"
     CDS             complement(39671..40957)
                     /gene="efmA"
                     /locus_tag="LK889_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBK5094599.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(41228..41572)
                     /locus_tag="LK889_000335"
     CDS             complement(41228..41572)
                     /locus_tag="LK889_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBK5094600.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(41709..42854)
                     /locus_tag="LK889_000336"
     CDS             complement(41709..42854)
                     /locus_tag="LK889_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5094601.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(43034..44008)
                     /locus_tag="LK889_000337"
     CDS             complement(43034..44008)
                     /locus_tag="LK889_000337"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK5094602.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(44088..44963)
                     /locus_tag="LK889_000338"
     CDS             complement(44088..44963)
                     /locus_tag="LK889_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBK5094603.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            45093..47000
                     /locus_tag="LK889_000339"
     CDS             45093..47000
                     /locus_tag="LK889_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBK5094604.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(47541..50819)
                     /locus_tag="LK889_000340"
     CDS             complement(47541..50819)
                     /locus_tag="LK889_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBK5094605.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(51090..51635)
                     /locus_tag="LK889_000341"
     CDS             complement(51090..51635)
                     /locus_tag="LK889_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5094606.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(51647..52339)
                     /locus_tag="LK889_000342"
     CDS             complement(51647..52339)
                     /locus_tag="LK889_000342"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK5094607.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(52358..52642)
                     /locus_tag="LK889_000343"
     CDS             complement(52358..52642)
                     /locus_tag="LK889_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094608.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(52655..53218)
                     /locus_tag="LK889_000344"
     CDS             complement(52655..53218)
                     /locus_tag="LK889_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5094609.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            53446..54108
                     /locus_tag="LK889_000345"
     CDS             53446..54108
                     /locus_tag="LK889_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBK5094610.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            54232..55428
                     /locus_tag="LK889_000346"
     CDS             54232..55428
                     /locus_tag="LK889_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBK5094611.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(55529..56905)
                     /locus_tag="LK889_000347"
     CDS             complement(55529..56905)
                     /locus_tag="LK889_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5094612.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(56942..57808)
                     /locus_tag="LK889_000348"
     CDS             complement(56942..57808)
                     /locus_tag="LK889_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBK5094613.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(57897..58922)
                     /locus_tag="LK889_000349"
     CDS             complement(57897..58922)
                     /locus_tag="LK889_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5094614.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(58940..59614)
                     /locus_tag="LK889_000350"
     CDS             complement(58940..59614)
                     /locus_tag="LK889_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5094615.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(59798..60691)
                     /locus_tag="LK889_000351"
     CDS             complement(59798..60691)
                     /locus_tag="LK889_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5094616.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(60765..62462)
                     /locus_tag="LK889_000352"
     CDS             complement(60765..62462)
                     /locus_tag="LK889_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5094617.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(62455..63768)
                     /locus_tag="LK889_000353"
     CDS             complement(62455..63768)
                     /locus_tag="LK889_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5094618.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(63768..67286)
                     /locus_tag="LK889_000354"
     CDS             complement(63768..67286)
                     /locus_tag="LK889_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK5094619.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(67579..68037)
                     /locus_tag="LK889_000355"
     CDS             complement(67579..68037)
                     /locus_tag="LK889_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5094620.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(68335..68853)
                     /locus_tag="LK889_000356"
     CDS             complement(68335..68853)
                     /locus_tag="LK889_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK5094621.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(68855..69472)
                     /locus_tag="LK889_000357"
     CDS             complement(68855..69472)
                     /locus_tag="LK889_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK5094622.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(69469..70323)
                     /locus_tag="LK889_000358"
     CDS             complement(69469..70323)
                     /locus_tag="LK889_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5094623.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(70320..71231)
                     /locus_tag="LK889_000359"
     CDS             complement(70320..71231)
                     /locus_tag="LK889_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5094624.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(71272..72606)
                     /locus_tag="LK889_000360"
     CDS             complement(71272..72606)
                     /locus_tag="LK889_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5094625.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            72814..73644
                     /locus_tag="LK889_000361"
     CDS             72814..73644
                     /locus_tag="LK889_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBK5094626.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(73888..74206)
                     /locus_tag="LK889_000362"
                     /pseudo
     CDS             complement(73888..74206)
                     /locus_tag="LK889_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(74328..74522)
                     /locus_tag="LK889_000363"
     CDS             complement(74328..74522)
                     /locus_tag="LK889_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094627.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(75027..75212)
                     /locus_tag="LK889_000364"
     CDS             complement(75027..75212)
                     /locus_tag="LK889_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094628.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(76062..76403)
                     /locus_tag="LK889_000365"
     CDS             complement(76062..76403)
                     /locus_tag="LK889_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5094629.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(76403..76633)
                     /locus_tag="LK889_000366"
     CDS             complement(76403..76633)
                     /locus_tag="LK889_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5094630.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            76920..77258
                     /locus_tag="LK889_000367"
     CDS             76920..77258
                     /locus_tag="LK889_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094631.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(77463..77702)
                     /locus_tag="LK889_000368"
     CDS             complement(77463..77702)
                     /locus_tag="LK889_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK5094632.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(77678..78025)
                     /locus_tag="LK889_000369"
     CDS             complement(77678..78025)
                     /locus_tag="LK889_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK5094633.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(78757..79338)
                     /locus_tag="LK889_000370"
     CDS             complement(78757..79338)
                     /locus_tag="LK889_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5094634.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
     gene            complement(79521..80147)
                     /locus_tag="LK889_000371"
     CDS             complement(79521..80147)
                     /locus_tag="LK889_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5094635.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(80169..81500)
                     /locus_tag="LK889_000372"
     CDS             complement(80169..81500)
                     /locus_tag="LK889_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBK5094636.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(81655..82056)
                     /locus_tag="LK889_000373"
     CDS             complement(81655..82056)
                     /locus_tag="LK889_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK5094637.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVFEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(82142..82348)
                     /locus_tag="LK889_000374"
     CDS             complement(82142..82348)
                     /locus_tag="LK889_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094638.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(82443..83945)
                     /locus_tag="LK889_000375"
     CDS             complement(82443..83945)
                     /locus_tag="LK889_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBK5094639.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(83963..84418)
                     /gene="argR"
                     /locus_tag="LK889_000376"
     CDS             complement(83963..84418)
                     /gene="argR"
                     /locus_tag="LK889_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK5094640.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(84516..85133)
                     /locus_tag="LK889_000377"
     CDS             complement(84516..85133)
                     /locus_tag="LK889_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBK5094641.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            85354..86727
                     /locus_tag="LK889_000378"
     CDS             85354..86727
                     /locus_tag="LK889_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5094642.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            86802..87914
                     /locus_tag="LK889_000379"
     CDS             86802..87914
                     /locus_tag="LK889_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK5094643.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(87986..89623)
                     /locus_tag="LK889_000380"
     CDS             complement(87986..89623)
                     /locus_tag="LK889_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK5094644.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(89791..90399)
                     /locus_tag="LK889_000381"
     CDS             complement(89791..90399)
                     /locus_tag="LK889_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5094645.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(90784..91935)
                     /gene="queG"
                     /locus_tag="LK889_000382"
     CDS             complement(90784..91935)
                     /gene="queG"
                     /locus_tag="LK889_000382"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK5094646.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(91954..92706)
                     /locus_tag="LK889_000383"
     CDS             complement(91954..92706)
                     /locus_tag="LK889_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094647.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(92847..93797)
                     /locus_tag="LK889_000384"
     CDS             complement(92847..93797)
                     /locus_tag="LK889_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK5094648.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(93798..94637)
                     /locus_tag="LK889_000385"
     CDS             complement(93798..94637)
                     /locus_tag="LK889_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5094649.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(94634..95380)
                     /locus_tag="LK889_000386"
     CDS             complement(94634..95380)
                     /locus_tag="LK889_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5094650.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            95618..96988
                     /locus_tag="LK889_000387"
     CDS             95618..96988
                     /locus_tag="LK889_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5094651.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(97043..97939)
                     /locus_tag="LK889_000388"
     CDS             complement(97043..97939)
                     /locus_tag="LK889_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5094652.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(98053..98223)
                     /locus_tag="LK889_000389"
     CDS             complement(98053..98223)
                     /locus_tag="LK889_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094653.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(98220..98897)
                     /locus_tag="LK889_000390"
     CDS             complement(98220..98897)
                     /locus_tag="LK889_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK5094654.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(98988..99893)
                     /locus_tag="LK889_000391"
     CDS             complement(98988..99893)
                     /locus_tag="LK889_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK5094655.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            100068..101522
                     /locus_tag="LK889_000392"
     CDS             100068..101522
                     /locus_tag="LK889_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5094656.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            101538..102569
                     /locus_tag="LK889_000393"
     CDS             101538..102569
                     /locus_tag="LK889_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK5094657.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            102576..103763
                     /locus_tag="LK889_000394"
     CDS             102576..103763
                     /locus_tag="LK889_000394"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK5094658.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            103919..105301
                     /locus_tag="LK889_000395"
     CDS             103919..105301
                     /locus_tag="LK889_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK5094659.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            105273..105659
                     /locus_tag="LK889_000396"
     CDS             105273..105659
                     /locus_tag="LK889_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094660.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(105859..106527)
                     /locus_tag="LK889_000397"
     CDS             complement(105859..106527)
                     /locus_tag="LK889_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK5094661.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        30579 a        22269 c        18800 g        35104 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtattata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccctccc taaacaaaca tcttttgtaa
     3901 gaagcctttt ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatcgtgtc
     3961 gtcgagttgt ttgaagaaag cgccgatttt ttgttgttcc tctttattcc tagtaaaagg
     4021 aaacttgata ttagcgaaat tctcatagaa taagtattct cgtacgcttc cttcagtgtt
     4081 gcgcttgacc tctttgataa atgttggtga cttgataaac tgaagtatga actcattatc
     4141 taagtcctcg ctcattttca caactacata aagagagcta acaatgaccg tcataccaag
     4201 attgttaaat gcaattgatc ctacgtttac tcttgctgga ttataggcga attcatttgg
     4261 ttcaacgaat ttataatttg ttttttctaa atcatccaac ctactaccat caaactgttc
     4321 tgtttgagaa attaaacctg ctttattact taccgaatag gcagggaagt ctaaatcttt
     4381 cgtgttcttt tttccagtct tcttactaaa ctctttgaac ttacgctgtt cccaatcttc
     4441 cgtaaatcca ggaaaccgaa tttctggcac ttttgctccg tttttaggga acattttttg
     4501 taagaatcct tttttcgttt cctttaataa atctagctta cgttgttgaa gagcgatagt
     4561 gtcgtccagt tgtttgaaga agttcccaat tttcttttgt tcttctaata ttggaaattg
     4621 tactatccat tttttcactt ctgacattgg tacgagattt gtaattgctg aacctggatt
     4681 agctcctgtg agttttcttt gcataaaatc tgcacgaata aattgaataa aatattctga
     4741 atctgtgatt tctttattta ttctaagtct aatcaaagcc tgattgaata cacccggttt
     4801 tatttgcttt ggtactcttg aaattctccc aattgttcca gcgccactca tgataaaatc
     4861 gcctggttct aatttaaatt tgattaattc attatatttt ttttcagtaa ttttatatct
     4921 tgtttcgtaa ttatcataaa tagcattttg ttgttcatag acaacaaaat tgctattaga
     4981 tacaaagaat tctttcttta aggcactccc aaatggtccc ctccttactc catctttttt
     5041 atcaagtaag cattcaaact tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat
     5101 ttctggttgt atatcattac tcatgtttga acacctccag cgcaccttgt aaccacgctt
     5161 cattttcagg tgttgtttgc agggaagaga tagcttctaa aagttctttt tctagcactt
     5221 gctgttcttg tcgtatcttc ttgatgtcat ttccgacatg aaccatatca attggttctt
     5281 cttcttcaaa cgtatctaca tagcgtggaa tatttaagtt atagtcattt tctttgattt
     5341 catcaaagtt tgccaaatga gcgtatttct cgacatcttt tctttcagca taggtttcga
     5401 ggattttttg aatgttttct tccgataatt tattctgatt ttttcctttg acaaattctc
     5461 gactagcgtc aatgaataat acgtctcgag tttggcggtt ctttttcaat acaatcaccg
     5521 tcgttggaat cgatgtcccg aagaacaagt tcgcaggcat accgatcaca gcataaatac
     5581 tgccgtcttc tagtaatttt tgacgaatga ccccttctgc agcgccacgg aataaaactc
     5641 cgtgtggcaa gacgatggcc atagtccctg tttccttcaa atgatagaaa ccatgaagaa
     5701 gaaacgcaaa atctgctttg gattttggcg ctaattttcc ataacgatta aaccgagaat
     5761 cgtctaagaa ggtcgtatct gctgaccaat tagccgaata aggtgggttc attactactg
     5821 catcaaatgt gtaaggttca tctgttggcc aatccttatt caacgtatcc ccattgcgga
     5881 gattcatttc ttcggcatct accccatgta aaatcaagtt catttttgct agattgtatg
     5941 tcgttgtatt taattcttgc ccatgatatt taacgttatc tgggtgagtt aagtagttac
     6001 gcacatttaa catcaatgaa cctgaaccca tggttggatc aaaaacacta aagaatcgtc
     6061 gttctttttg atccaacgtc acgatttgtg ccatcatatc agaaaccata tgaggcgtgt
     6121 aaaattctcc ggctttcttc cctgcttctg aagcaaattg actgatcaga aattcataag
     6181 catccccgat cacgtcccca tcgtgaccta aaacttccac gtcattcaat ttcttaatga
     6241 cttcggtaat cgtcacattt cgttgttgct catctgttcc tagttttttt gattggagat
     6301 ccacatcatc aaataaccca ttaaattgat tgtaggtaga tgccaattgc acaaaggctt
     6361 tgtttagatc attcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa
     6421 gatactctgg cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc agatcttctt
     6481 ttgattcttc atctgaaagt aattcgcgat acaacatcgt ttgtttgctt accgtatcat
     6541 attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat
     6601 atttatagaa aatcagtccc aataaataat ttttatattc tgacgcatcc attttgctac
     6661 ggagattatc tgccgcacta aataattttt gatttaattc tgcacccaaa aagatacact
     6721 cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactcc cttttagaaa
     6781 aagcaaaaaa aaaagaaaga aacttattct tgctaataaa ctacgcgaaa ggagtctaac
     6841 gttgtttgat ataacttaga ctgatcgtta acttattttt tatatttcta catagttatc
     6901 tttagaacca tttcagatac atatgaaaca attacatatg attttttcat aaaaaaacag
     6961 tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta aaatcactac
     7021 ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc gtttcatttc
     7081 agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca gtagcttatc
     7141 ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt aaataaatga
     7201 aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta atcagttacc
     7261 tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa ccgaagtgga
     7321 atatctttac caactttctc tgtcactgca ttactgatac ctgttagatt agctaataaa
     7381 tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt gattccttta
     7441 tcttcattta caagtttcca agtaggtggt atagctgtgt atgtttctac aaatgaatta
     7501 agtttttcat taagtgactt ctcttttata tttccatcta aaaactctgt atatatggct
     7561 tgagctatac taaaacaaag ctggccttgt tctaataatt ttacatcatt agttagtaaa
     7621 gctgtgttca cgttatcaat cgcttcaaga agagtatctt tggtgtccct aacagcttta
     7681 ggtgtgcctt ctggaatttc cctacttgat acctcgtcca ttttatcttc cataaacccc
     7741 ttaaaatcat atctttctcc caaatccata gaagtttttg agaaagcttt catttctgag
     7801 tctatttctt caataatatt taacatgtct gatacatatt gtttaggctg attcgttttt
     7861 aatctatatg tggtaaatat ctcaattcct ccaattatca ataaaattgc caaacaaatc
     7921 cataataatt ttttcttttt catttttcct cctatacact gctgttataa cagtgactaa
     7981 tgtaaagatt ttcccagcaa tcatcagact attatttaca gggaatcaaa aaaactcctt
     8041 agtaagctta cacctttagg agttcctgtt tgaacggtat gcataataca tttaaatcaa
     8101 tttaacatta acacctcagt atatggtatc tctacatata cacggtcttt ttttggatag
     8161 tactcgacat ttatattgat agcacttcca ttgtctaacg tagctttagc ccggtacgtg
     8221 ttatcaccat tatcatgagt gatcgtcaca tcatcacatg tccgatttaa gttatactgt
     8281 tcttgtaaaa tctgagttac caatggtaca gatgacttct ctagtccaga tgaacccgtc
     8341 attcttgaaa aaattatacc tataatagcc aatacaacga tacctataat gacaaacttc
     8401 ttattcatat ttactttatt caacttttta tctcctatct ccaaaatgat tttctctcat
     8461 cagtaactca ttgacatccc caatagtaca ccaaagatgg ctccagctac ccctgctatc
     8521 atcaaaatcg tacccatttg ttcatctttc tctcgatata agtaacccat aataacagca
     8581 acagcaccaa ataccacagg aataaatgct aatgatacta ccatagaaat ccaaccaata
     8641 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
     8701 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtaat
     8761 gctccgcaaa actggcaata cttactttca tttgttattt ctttaccaca ttttctacaa
     8821 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gctaactgtt tactttatta
     8881 aatacatcag aaaacagctc atttattggt acacttaata tctcagaaag cattttcatt
     8941 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     9001 ttcatttttt tggctaactc agtttgggaa agtccttttt cttttctgta tcggctgaga
     9061 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
     9121 ttcaactcat aaaatatata aatacttcaa atgtcattac aaatacaaca acttgttgta
     9181 ctctactgag aaggggggtg tattaatgaa acagaagcaa tatgcaaagg tcattagaga
     9241 aaagcgaaaa gcactcgggt ggacacaaaa agaattagct aaaaaaatct tctctacaca
     9301 acaggcagta gcaagatggg aaaattctgt aacagaacct aacctcgata gcttaacagc
     9361 tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg acatagatta
     9421 tgaggaagag tttttggctc tctatcgctc tcttagtact gaagatgcaa ttcgaacaat
     9481 tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg
     9541 aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga
     9601 gctatggata acctgtctag tttaatataa ttgggtctct tctattcctt actctccgtc
     9661 catcatctct tgttacactt gtaaattgat aaataggaac taattcaact cctgataata
     9721 tcaattcttt tattacttca tctatccctg cccctttcgc tagatatact aaaaaactac
     9781 tgcttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca agagtgaatc
     9841 cttgtttaac aaagtagcct ctgttttcta ttaaagcatt tttcacgctg ttatccctga
     9901 catttcgtag gcgttcacaa acagtgttaa taagttcttt ttctgtcatt agatatgtcc
     9961 cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct
    10021 tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac
    10081 atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg
    10141 gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat
    10201 agacattgct tctcttatgt ttaaagtttc ctaacgcttt ctcttccttt aacctattta
    10261 ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa
    10321 aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaag
    10381 aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt tccttactat
    10441 catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat
    10501 aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt
    10561 ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc
    10621 aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat
    10681 tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg
    10741 taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg
    10801 acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca
    10861 tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat
    10921 tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac
    10981 tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca
    11041 tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat
    11101 tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag
    11161 caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt
    11221 ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat
    11281 tgatagataa aaccttttct atgcttaagt atctactatc atctttactc aaatctacta
    11341 ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca
    11401 caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg
    11461 aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat
    11521 ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt tcttgatcta
    11581 cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg
    11641 ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat gttttagtct
    11701 tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat
    11761 ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc
    11821 gttcctactg atacacctag atattcactt gcctgctgat agtccatata ctctagagtg
    11881 actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt
    11941 tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct
    12001 ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa
    12061 agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca
    12121 cataatacct cgataaaaaa ggggtgtcaa cagctttgta aaatttattt acaacacccc
    12181 ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt ccaccaataa
    12241 attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa
    12301 aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaaa
    12361 ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg
    12421 ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt
    12481 tgggaattat tctttataga gatagcattg tactagatgg tataacattt ccagataaaa
    12541 acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc
    12601 tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta
    12661 ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca
    12721 tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag
    12781 aactactaga aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc
    12841 ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat
    12901 caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag
    12961 tactccttgt aaactaagag gagatgattt aatggcaaaa atatcaaagt acaaaaagaa
    13021 aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga
    13081 aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga
    13141 cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga
    13201 agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc
    13261 aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc
    13321 gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag
    13381 ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata
    13441 tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa
    13501 tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg
    13561 taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga
    13621 agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa
    13681 aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc
    13741 tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca
    13801 gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt
    13861 tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat
    13921 tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag
    13981 tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa
    14041 tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg
    14101 tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt
    14161 ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta
    14221 taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt gagttcgact
    14281 gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac gatcatttca
    14341 gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc ttctgagatt
    14401 tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc attgaatggg
    14461 atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat atgagagatc
    14521 gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc tccgccgtct
    14581 tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg gatcattttt
    14641 gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat ggtgcctcgt
    14701 gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc acctaggttt
    14761 ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt tactaagttg
    14821 tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc ttttcttgta
    14881 aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc cgtacaacct
    14941 tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc gaattgcccc
    15001 gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt gacgcctttt
    15061 ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc taatttgttg
    15121 tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta ttctttcaaa
    15181 gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt catattatgg
    15241 tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg ttcagcttct
    15301 ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc ccagtcacga
    15361 gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat gtctgaaagg
    15421 tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt cagacggaat
    15481 tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac ttcttctgtc
    15541 aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa tttatattct
    15601 tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat ggaaagctgc
    15661 ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg cacatttgat
    15721 ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt ggtcgggatc
    15781 tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt ctgcttcttc
    15841 tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac tgatggagca
    15901 gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga cttgcaattc
    15961 gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt cagtcaagct
    16021 tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata aattgtctaa
    16081 tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca actaaacggt
    16141 cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct gatacttcaa
    16201 ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc ccttccatgt
    16261 ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca acgactcctg
    16321 tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct tgtaattttg
    16381 gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata ccgagttttt
    16441 gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt gtccctgctt
    16501 cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa tcgatcattt
    16561 cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca tataaaacac
    16621 caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca cagtctagtg
    16681 cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg atgaccccgc
    16741 cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct aagacattgg
    16801 atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct gcatcctcca
    16861 tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct gttcgttttg
    16921 ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc actatctcgt
    16981 ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag cttgtcgtcg
    17041 taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc cgtacttttt
    17101 ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca ccgcgatgaa
    17161 cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt tttggctttt
    17221 gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct tgaaataaga
    17281 taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc cttcgattac
    17341 ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag aaccgaggaa
    17401 tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca tcagatagta
    17461 catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta cttcgttttc
    17521 atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca tcaagacacg
    17581 actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga taccattaaa
    17641 ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga tcgtatgagg
    17701 ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc tgattgccac
    17761 gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt cagcgatcac
    17821 atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta aaatgtgccc
    17881 acggcgattc atataagaag taatattttc tcctaattga tctacggctg aatatttgat
    17941 ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt ttgctacttg
    18001 ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat ctgccacgat
    18061 caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata aggactcgag
    18121 tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt tcataaaagc
    18181 tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca tataatattc
    18241 ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa tgactccttt
    18301 ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt ggacaagcac
    18361 aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga tggtttgtgt
    18421 ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat gcggcaatgg
    18481 cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat caggttcgcc
    18541 tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt tttctttcat
    18601 tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt tactgatacc
    18661 agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca tgactactcg
    18721 gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc gttgatagtg
    18781 ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt tagcaacgac
    18841 tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa atgtaggttc
    18901 taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa aaccttcgtt
    18961 cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt catctaactt
    19021 tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat tgatcgctgc
    19081 acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca tagctaaaaa
    19141 taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc cgtcaaacaa
    19201 aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag ataaggtaga
    19261 ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca ttgttaggtt
    19321 tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta tttctaagac
    19381 agacatgcat tataccccca aattctcttt cgttctcact cattttagac taattgagaa
    19441 tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat taaacaattt
    19501 tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac agacttattt
    19561 ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta acagagccgc
    19621 cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca atcagttttt
    19681 tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg taaggagagt
    19741 tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg taagcgaagt
    19801 ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct aaaagtgatg
    19861 ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca tcgaatgttg
    19921 ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt agaattgtga
    19981 tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca tcttttaatt
    20041 cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg acagcaccgg
    20101 cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga atatgctgga
    20161 agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca tagtcatcga
    20221 atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca tgttccaaaa
    20281 tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct gctttatttg
    20341 ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc agcaaaagcg
    20401 ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat cctattatct
    20461 tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata cgattagtaa
    20521 aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc cttcctgcca
    20581 agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa atccgatcat
    20641 cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag gtatcaagac
    20701 cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca gtacaccagg
    20761 gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg cggataaaac
    20821 aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt tagtaaatgg
    20881 aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac tgatgatcga
    20941 tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag agtaaaggat
    21001 caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg tcatatatag
    21061 tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat ctagatatgt
    21121 ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt tctttgatga
    21181 acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc aatgaaccaa
    21241 aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg atgttatagc
    21301 gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag gtcaaagttg
    21361 ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc gaatcagctt
    21421 ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt tgtctgaaga
    21481 cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg actttgttgc
    21541 agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa ttcaatttct
    21601 tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc aatgcgctag
    21661 tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg ataccgacgc
    21721 gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct tctaacccga
    21781 ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg ccagctaatt
    21841 ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg aaatgctgga
    21901 agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat tttgtaatat
    21961 cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc aggagacgga
    22021 ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc cctttttcaa
    22081 cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg aatgtctttt
    22141 tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat gatttttcaa
    22201 acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa cttccgtgtt
    22261 accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg gacttagctt
    22321 ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct tacgctcgct
    22381 tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag caaacagggc
    22441 tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt tcatatagga
    22501 ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt gattttctga
    22561 ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat ccacgcattc
    22621 atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt aacagaagaa
    22681 actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc aacttcgatc
    22741 agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa cgtgatgtct
    22801 ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc attaaataag
    22861 atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca gttctccttc
    22921 ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga tcagtggtcg
    22981 cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg taaactcgcc
    23041 tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga acaggcgacg
    23101 gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg gtacttcttt
    23161 gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt tggctttttt
    23221 gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct ccttgaagat
    23281 cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc gatcatgttc
    23341 cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt taaaggaagt
    23401 aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat gatcaaaccg
    23461 cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat catacgatag
    23521 attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa gttttcaccg
    23581 ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct tacgttgaat
    23641 cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg ctgataactc
    23701 attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa ctgataagat
    23761 ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa gaagatcgtt
    23821 cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca agagattcca
    23881 gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc aaagtcatct
    23941 tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc aatcagccaa
    24001 aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac gatcagtgcc
    24061 atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa cgcaccgaat
    24121 cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa tttgagcaag
    24181 agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca gatccatttg
    24241 gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca gacaacttgc
    24301 atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc tacatataat
    24361 gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata atcaatacga
    24421 ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta aaaaccatac
    24481 tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga catttgggaa
    24541 aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc ctactgctta
    24601 tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc tgagattctc
    24661 ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg caatctctag
    24721 cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat ggaaattcaa
    24781 cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc gaccgattcc
    24841 ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct tatcttggat
    24901 attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg cttctcttac
    24961 aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca tcaaagagta
    25021 tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg attcatgttt
    25081 cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat gcactttagg
    25141 aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa aggtgtggga
    25201 agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt gctgagtttc
    25261 ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt gcttagttgg
    25321 aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc gctccaactg
    25381 tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg ctctggaaaa
    25441 atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg caccacttcc
    25501 ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa aaaaccatct
    25561 ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga tgaagacaat
    25621 cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta ttcgttcatc
    25681 ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac tttttgaaaa
    25741 ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga aatctgttta
    25801 aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg attcttatga
    25861 tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg ttgcatgatc
    25921 caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt tgtacttatt
    25981 ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc gctgttatct
    26041 tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct gatctttcaa
    26101 ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc atcactgtaa
    26161 ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca ttgatctgct
    26221 atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat atggctcatc
    26281 cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta acaggttatc
    26341 ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct ttatctcaag
    26401 tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt ttttatccaa
    26461 gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt actactgttc
    26521 attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa gttgcggaat
    26581 gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt ttcttaatta
    26641 aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa tcgattcaat
    26701 aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa aacagctgtt
    26761 catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac accgtttacc
    26821 aagaggtcgt aaaagaagtg gtcagcttca agaaataaga aggaatttac gaaaattgct
    26881 tctcaaattt ttgtgaaatt tcggcttatt tccgtagaag ctacttctgg aacaccgttt
    26941 attcgaaaca agaggtcgta caagactttt gtcacgacct cttgttcttt tgtcaaaata
    27001 ctggtttagt gctactatgt tcgagtatgc ttttagtatt tattgacgaa aggaaccgat
    27061 caaatgaaat cattgtcaca tccaataaaa tctgtaaagg attcagcggt ccgcgaaatc
    27121 ttacccggtt tactgctttc ttttgccatc gctgccttca gcaggcttct tgctatttat
    27181 ctgccacagt taggtggagc gactattgct attttattgg gtattttatt aggaaatact
    27241 ttttttaaac agcctgttct agcaatcggc acaaaatttg ctgaaagcaa actattagaa
    27301 ttctctgttt ttttactagg tgcaactgtc acagctcaaa cgattgctca aattggtgta
    27361 aaaggaattg tttttgtgtt actgcaaatg tcagtcacta tcatcggtgc ttacttgatt
    27421 ggtaaaaaac tgcaattcag cgatacttct tctttgatga tggctggagg aaacgctgtt
    27481 tgtggttctt ctgctattgc ttcaatcgcc ccttccatcc atgcgaaaga ggaagaaaaa
    27541 ggacagatca tcacactagt caatctactg ggtactgtga tgatgttgac tctccctttt
    27601 cttggattac agttgtttgg tggaaatctc attgaaaaaa gtgcactttt gggtggtaca
    27661 ctccaatctg ttggtcaagt agtcgctggg gcaagcttac ttgacaattc tgttgtcgaa
    27721 ttcgccatgc tatttaagat tctacgtatt atgatgctcg ttgttgttgt ctatctgttt
    27781 gaaaaattca tgcacaagaa acaaccagct ctgtttgaaa cgccaactga tacgcagaaa
    27841 aaacgaaaat taccgcttcc ttggtatgta ttagggtttg tgatcttttg tttggtcaac
    27901 agcttgtttt ctttacctgt agttattgac caatcggcac attttatcag cagctggttt
    27961 gagatcactg ctttagcggc aattggtctg cgtttagact ttgcaaaatt catgaaagaa
    28021 ggtacacgct tcttatctta tggattagct gtcgggcttc tccaaacagt gatggcttta
    28081 ctatttattt ggctgatcaa agtttagaat gaacatgaaa aaacagttgt gattctcgca
    28141 tggaacaatg tccgaatcac aactgttttt aattttgcag atatccttct aacaacaaca
    28201 attggcgttt gatttcaacc ccatctattg cgtttcccat atatcctgtc agttttccat
    28261 tttgcccgat cactcgatga caaggaataa cgattggcaa tggattggtt cgatttgcct
    28321 gcccaatcgc tcgcacagct ttcggactat caacagcaac ggctacttct ttatacgttc
    28381 ggatttctcc ataaggaatg gtcgacaaag ctttccagac attttgctga aaaggcgtac
    28441 ctctctttat agataaagga acatcaaatt cttttctttt tcccgcaaaa tattcttcaa
    28501 actgttcttg tgtgcgcgtt aaaattcgct gattcgctga ttcgctttct gaaattggct
    28561 gaaaagatac agcagtcaat ccggattcat ctgcatctaa ccataaaaag ccgataggtg
    28621 tgtgtatttt cattttgttt gctcctagtc aatagactgg aacttcctcc tttttctcta
    28681 tccaaaacta cttgccaact aataaaaatt gttcttaaaa gtttgataaa ccgaggaaac
    28741 atggtaaact aaatacgtta gtttacaaaa agaatacctg ttcttttacg taaaatcaaa
    28801 ttttaaaggt gttatttaag gtttattaaa ttttgaatgt aaaccatata taaacatacg
    28861 attgaaagga tagaaacgat gaaagtatca attgttgtcc catgttttaa tgaagaaaaa
    28921 actgttcctc ttttttttga tgaggttgaa aaattcaaag gtgcccatga gttcgaatat
    28981 ttgtttattg acgacggatc aaaagacggt acgttagcag caatcaagaa gctagcctca
    29041 gcttatcctt atgtccgctt tgtctcattt tccagaaatt ttgggaaaga agcggctctg
    29101 tacgctggat tgcaagcttc tacaggagat ttagtgaccg tgatggatgt tgatctgcaa
    29161 gatccgcccg aactgcttcc tgagatgatc aaccggatca ctacttctga tatcgattgt
    29221 gtcggcaccc ggcgttcgac tcgtgaaggc gagcctgcga tacgcagttt ctttgcgaaa
    29281 aaattctacc agttgatcaa taaaatcagc gatacagaaa tggtcgatgg cgctcgtgat
    29341 ttccgcttga tgactcgtca aatggtggat gccgtcctag aattaacaga atacaaccga
    29401 ttttcaaaag gattatttag ctgggtcgga tttaaaacag aatatatttc ttatgaaaac
    29461 cgcgaacgtg tggctggcga aacatcttgg tccttctgga aattattcaa ctactcgatt
    29521 gatggtatcg tgaacttctc ggatgcaccg ttaacgattg cttcatttat cggcgctttg
    29581 tcttgtgtcg gttctggtat tgcaattttg ttcattattt tgcgggcatt gttgtttggt
    29641 gatcctacat caggatggcc ttccatggtc tcgatcttcc tgttcattgg tggaatccaa
    29701 ttgttatgtc ttggaatcat cgggaaatat attgggaaaa tcttcttaga aacgaaacag
    29761 cgtcctgttt atatcataag agaaacagaa aaagtgaagc caaatgaatt gaagcctcac
    29821 caataatata cgtcaaatga agtaaacatt tttttaaatg aaaaaggact gtgacaaaag
    29881 tcttgtcaca gtcctttttt ccgaataaat ggtgttccag aagtagcttc ttcggaaata
    29941 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    30001 gctgaccact tctatcacaa cctctttcat atcttattac ttcttcattg ctgcttttag
    30061 tgctgctgct aatgggcttt cttctggttc ttctgcttgt gcatattttt tcagcaagcg
    30121 tcgttcttca tgtttggtca ttttcttctt ctgctctttt ttatcaagca atttttctgt
    30181 cgttccatcg tatttacaac ggaaataagc accgttttta ccttcgatga tttccatttt
    30241 ccggtgacat tgcgggcatc gatgattaga gacttttgga tcttttcttc tgcggtagtt
    30301 gcaatctgaa ttggtgcaga catagatttt gccgtcacgt gtgtttttct cgcgcagatt
    30361 ggccccacat tctgggcatt tcttttgggt aatcgagaaa tcttgatatt cttgtttgct
    30421 tgatttgatc tcattcacca agcgttttgt ttctttttcg attccattca agaaggttcc
    30481 tgcttgtttc tttcctgcag caatagcttc aaggtctttt tcccagcttt ctgtcaaatc
    30541 tggtgttact aaagagggat ttaccaatac taataattgc ttacctttag gagatacttg
    30601 cagtccttga ggggtacgtt ccatcagttc agatttgatt agtttctcga taatctctgc
    30661 acgcgtagct ggggttccca gactgtattt ttccatttga ccaagcaagc ttgcttctga
    30721 caatggttta ggcggtgcag atagttcttt tttgatggcg aaatcgggat tgattctcat
    30781 tcctttttcc catttgactg tttccgtctc agtttcagtt tcgcctttcc atcctgcttg
    30841 gacgacacga ttttgccgaa aaatgaatcg ttctttacca aattcaaccg taacttttgt
    30901 ttggctgact ttatggggtt gagcaaacag tcctaaaaaa cgagagacaa tcatttgata
    30961 gatttttcgc tcttcattac ttaatttttc ataacgtgct ctgttttcag ttggcaaaag
    31021 tgcatggtga tcggtcactt tactgtcttg gaatactttt gtctgttgga cgatggctcc
    31081 gtttttcaaa tactgcttga cctcaggagc aaagtcgctg actgcttgca atcgttcttt
    31141 catcgtactt ttcatgtcat ttgtcagata tttgctgtct gtacgcggat aagtaacgat
    31201 cttatgggtc tcatacaaac tttgtacaag acttaatgtt ttctttgcag aatagccata
    31261 gcgttgattg gcttctcttt ggatctctgt taaatcataa ggcaatggcg cagattccgt
    31321 ttttaatttt tcttggactt ctgtgacagt tcctttgctt gtactgatca attgaacaag
    31381 tttttgtgct tcttcttgtg tttttaaagc aaattgattt ttttgaagca ttttggcttg
    31441 ttcttttcca gcttgcaacg tgatcgtgaa atacgtttgt ggacggaact gctcgatttt
    31501 cttttcttgg tctctcacta atgctagtgt cggtgtctgc acacggcctg ctgataaatt
    31561 atccgcgtac ttcacagtta aggctctggt cacattcaat cctaccagcc agtccgcttt
    31621 tgcccgggca agagcagatt catacaatgc atcatattct ttcgcaggtc ttagttgctt
    31681 gaatccagtt ttgattgctt tatctgtttg agaagagatc cataaccgct tcactggttt
    31741 attgaaatgc acatactcta agatccagcg agcaacaagt tccccttctc ttccggcatc
    31801 tgtcgcaatg actgcttctg agacatcttt tcgctgagcc agctgcttga ttgcttttaa
    31861 ttgatgtcct gttttaggaa gtggtttgat tcctagtttt ttagggatca ttggcaacgt
    31921 gtccatttgc cagctctgcc attccttatt cagatcttcc ggcattttca atcctaataa
    31981 atgaccaagg gcccaagtaa cgatcacctt gggtccttca taataatttt tgtgcttttg
    32041 attagcacca agcaccttgc ttaggtcttt cgcaacgctt ggtttttccg ctagaattag
    32101 ttgtttcata aaattccttt ctgctttcta ctgtgatggg ctgatgattt ttcaacaacg
    32161 caagtccctt gtattgctgt aaatcatcgt cacactcttc acaccagcgc tcatctccgt
    32221 cattccaaaa ggcgctgaga aagttacttt ttgcattgat atattcataa ttttgatgga
    32281 tttcgttcca ttgtttttcg tacgcttgtc gtgcttcttc gaaagactcg aatgtttctt
    32341 cttcttcaat atcttcttcc cagtcttcaa aaaaccacca aggctcattg tccccgaaca
    32401 tggtaattac ttgatacata ctcgtgctcc ttctttcagc ttttctagac tcatcttagt
    32461 cacttttcaa gaaagttgca agaattttct cttataaaag ggtcatttaa tgaacatctt
    32521 aaaattgtta caaatgaatc tgattgttta aggcaaagta gcgataaagt tattgatcat
    32581 ccaaaaatag ctgtcttctg gatcgattgt tggttctttt gtaaagtatc ctaaaaaacg
    32641 taaggaaaga taaccgtgga tcatactacg aagttccctg cttcgatgaa ctttttcttc
    32701 tttcgttaat tgaaaagcct caaatatctg caaaatgatc tgattcgttt catggattcc
    32761 atctaataaa acagcttcat tggtatgcgg tacactgatc agtaactcat acactgcttg
    32821 atttgaaaaa gcaaaggatc gataagtttc tgcatagatc tgcagtgctt cctgaccact
    32881 ttttcccaca agagcccgtc gcagctggtc attaagttcc tgcagaagtt tcgctgttag
    32941 cgctgttttg acatcacgaa tatttttgaa atggttatac agcgagggag ccttgatatc
    33001 caagcgtttg gctaaatgct gaaaggttat ttgctgaaca tccatctctt ctgctagttc
    33061 tcgaaagcag tcgatgattt tctcttgaga cagatttctt tttttcattt ccatgtttcc
    33121 tctcataaaa taaatccttc actctttcag tttaccacga aaaagaaaga gaaaaaagag
    33181 actttcattg ataaactaca tattgtagtt tataataaag agctgtagat gaaaattttc
    33241 agaaagaagg agtctaattg gctgcaagaa taaaggaaaa attatttagg aaaagagaaa
    33301 cttgggaaca aaacttgttt gtcttatggt ttggaacatt tatggctggt atcggattta
    33361 gtttagtcat gccttttatg tcactttaca tagatacgtt aggtaattat agtacgtcac
    33421 aattgaactt ttggagcgga ttgacttttt catctacttt cttggtgact acgcttatct
    33481 ctccttggtg gggaaaatta gccgaccaaa aaggaagaaa attgatgctg ttgcgtgctt
    33541 cattaggaat ggcaatcgtc atttctttga tgggttgcgt gactagtgtg tatcaattag
    33601 tcgcattgcg tctgcttcaa gggatttttt ctggttatat cagtaatgcc actgcccttg
    33661 tcgcaactgg gacgccaaaa gaaaaaagtg gtcaagttct tggaacctta gcaactggtt
    33721 ctgttacagg aacattactt ggaccgcttc ttggcgggat ctctgcttca gcgtttggct
    33781 atcgccctac ctttttcatt acagggatca tcttattttt cgtcttcttg ttgagtctgt
    33841 ttttcgtcca tgaaaaattt gtcccagtga aaaaagaaaa catggtttct gccaaacaga
    33901 ttttcaaaga cttgaaatat ccacacgtcg tcatcggtat gttcgtcaca acgatgatca
    33961 tccaagcatc caacaattcc attagtccaa tcatcagtct gtacattcgc caattgctgc
    34021 atggacatgg gaatgttacg ttgatcagcg gaatcattgc ttcgatacct gggattgcca
    34081 ctttgatagc cgctccaaga tttggtcgct taggtgacaa gattgggagt gaaagaatac
    34141 ttgcgattgg tttgggtttt gcgattctgg tttatatccc aatggcattc gtcacgaatg
    34201 tttggcaatt agctgcctta cgttttttga ttggtatctc tgatgcttgt ctgctccctg
    34261 ctgtccaagc attgatcaca aaatattcgc ctcatcaagc tgctggacgg atcttcagtt
    34321 ataaccaatc tttccaagca actggtaacg tagtagggcc tatgatcggc tccagtgttt
    34381 ccagtgcatt tggttatcgt ggcgtctttc tttcgacttc tcttcttgtt ttggtcaact
    34441 tcttgcttgt acgacacaat acacaagaga tccatcagca agaaactgca gaagaaaaag
    34501 cttcaggagt ctccatagct tcagggcact ctccttctca tagacaataa gcgtaaatga
    34561 aaaacactgc ttgaacagtt gtcacagact gtccttgcag tgtttttgtt taggcctgtt
    34621 ttttcaagaa ctcagataag aggaataatc ccttttccaa ggtttctttt ttacaacagt
    34681 aacctagtcg ggcatgcttc ggtaaatcaa aggcacttcc tggtactaat agtactcctg
    34741 tttcttctaa taaattgata caaaatgtct ggtcatcttc ttcgatggct aatttaatga
    34801 aagaagtaga gacgtaattg ggagcgacaa gttcaacttt cggttcattg gctacccatt
    34861 gttttaaaat cacaaggttc tctgtaatga tctttttgtt tcgcgccaat attttttctt
    34921 tattcttcaa tgcatgtaca gcaagatcat ctgacaggac tccgccgcaa atcatcgtgt
    34981 agtctcgata ctttcgaaaa acttctgcca actcaggtcc agtagctgtc cagccaatac
    35041 gaatacctgg aatggagtag gttttggata gggaattggt tgctatacct ttttcataga
    35101 catctacgat cgacaagaat tcacctttat ccgttaaagg cgcatatact tcatctatca
    35161 atacatatgc atctactgta cgagcaattt ctgcgatttt ttctaaagtt ttccgatcta
    35221 gcaatgtgcc tgtaggattg tttgcactat tcaaacagat gatttttgtt tcaggtttga
    35281 ctaaggcttc tagctgttct aaatcaaact gccaatcttc ttcttccttc aaatgaacaa
    35341 agtcaacagc ggcacctaaa gatttgggga tgtcatataa ttgctgataa gtaggaagca
    35401 tcgaaacaac atggtctcct gcttccacta acgcgtatag agctaaaaga ttagcaccag
    35461 tcgctccatt cgtttgtaag atgttttccg gatcgacggt ttgataaaga gaagcgactt
    35521 cttgtttgaa aacatctgat ccttcgatcc agccatagtc caatggttcg tttttgcgtt
    35581 tttcgaaaaa tttacttacg ctagtcccat ctaagccaac gagttcgtct aatgtcaatg
    35641 cttcgatcga gctttgtgca atatcatata ccgctttctt ttcccattta tttagccatt
    35701 cttctacacc gaaatttgcg attttcatgt ttctttcccc cttatatctt gatccagcct
    35761 acagcaagca acccgattga aagcagtgcg gccacacaaa tcagcccaat cattactttt
    35821 tcttttcccg taatttgatg tttggtttca cgacaagcga tccagtagaa gcaaaaacca
    35881 ggaagatagg caatactaca taaaaggaca taagaaaatc ctgctaaaat gatgcccatc
    35941 aattcaaaaa ctacagcaat cccaccgatc actacttgtt ttttgattcc ttcttttaaa
    36001 gaaaactgga cttgatagat cccgacaagt agataacaaa tcaaaattgc cgctgtacat
    36061 aaagaatagg caaattgata ggcataatca gtaactaaga aagtcaataa gaatagatta
    36121 gtcaagattg cagtaacaac taacgaataa gttggggcat tttttttatt cgtttttccc
    36181 caacctttcg gcaacagacc gtctcgagcc atcaacatcg ttgtttctgc tgggagcatc
    36241 gtccaagaca accaagaacc aatgatcgaa ataatcaagc cgatattgat cagcgctgct
    36301 ccccaatttc ccacgattcc ttttaaaata tttgccatag caggttgtga aatagttgct
    36361 aactcttctt gagataagac gccgtatggc aagatcgaag caaggatata gataaacaat
    36421 aaacaaagca gaccaatgat cgatgcttgc tgggcttctg aacgtttctt cgcgcggttg
    36481 gctaaaacac ttgcgccttc gatccctaca aagacccaca tcataaccat caaacaacct
    36541 ttaatctgca accagatcgt tccttgctgc cctgttccat tcactagatt atcagatacg
    36601 ttaccccaaa aatcagcagt gaatatattc actttaaatg cgataaatac aaatattaaa
    36661 aataggaata atggcactaa tttacagatc gtcacgattg tattgatgac gctcgctcct
    36721 tcaactccat tgtttactaa taaagtcaga ccccaaataa aaatacttgc aaataaaata
    36781 gaaggaacat tttgtccacc tttgaatatc gggaaaaatt ctcccatggc gctcatcagc
    36841 atcgttgcaa aagcaacatt tcccagccac gcagacaacc agtaacccca accactgatg
    36901 aagccaccta atttaccaaa tgaagcttcc gcgtaaccga aaataccacc atctagatct
    36961 ggacgctttt ctcctaaatg attcagcgat aataccaaag ctaaaatacc aatcccaacg
    37021 attatccatg aaaggatcgc tggccctgga gctgcagaac tagccagatc acttgtgatc
    37081 ccaaacacac cgcttccgat tgacgaactg acaaccagag caaccagtcc ccatttgcta
    37141 atacccgtcg atttcttttc catttccatc accctcactt ataaaattga acaaatcact
    37201 tgcaaaagat tcccttctat tttgcaactg ctcttgctta acaaaaagca actttattca
    37261 tattattgga tattatgaac tttttacaca agtatttcaa gtataaaatg aataaaaatt
    37321 aattttattg caaaaaaaca gctatgctat ccaaaagaaa aggtgtttct tttgttagcc
    37381 atagctgttt tgattcattt aatatcgctt aaacgtattg taaaactttt gtttcttgat
    37441 atgcgtggtc gatcaaacca ccacccagac attccattcc atcataaaat acaactgctt
    37501 gtccaggagt gattgcacgt acaggttcgt cgaatacgac ttctgcacga ttatcatcca
    37561 ataagcgaac tgtcacagga acgtcttgct gacgataacg gaatttcgct gtacacttga
    37621 attcttttgg cattggttca ttcgtcgtaa agtggatttc acttgcatcc aagcttgtgg
    37681 catacaatgc aggatgatgg aacccttgcc ccacatataa tgtgtttgtc gctagatctt
    37741 ttcctacaac aaaccagggt tcttgcgaat ctcctccgcc accgatccct agtccttgac
    37801 gttgaccaat cgtataatac atcaagccag catgttgtcc tttgacttca ccatcaagag
    37861 tgaccatatt tccttttttt gctggcagat agttgctcaa aaattgtttg aagttctttt
    37921 caccaataaa gcagattccc gttgaatctt ttttcttagc agtcgcaagg cctgcgcgtt
    37981 cagcaattgc ccgtacttct gatttttcca tgccgcctag agggaacatc gtttttgaaa
    38041 gctgttcttg tgataattgg ctaaggaagt aagtttgatc tttattattg tctactccac
    38101 gaagcatgtg gaccacgccg ttttcatcgc gagtgacttg cgcataatgt ccagtagcta
    38161 cataatcggc gccaagatcc attgcataat ccaagaatgc tttgaatttg atttctttgt
    38221 tgcacatcac gtctgggtta ggtgtacgtc ctgcgcggta ttcggctagg aaatattcaa
    38281 atacacgatc ccaatattct ttttcaaaat tcacagagta ataaggaatc ccgatttgag
    38341 cagctacttt cgctacatcc ttataatctt ccgtggccgt gcatacaccg ttttcgtccg
    38401 tgtcatccca atttttcatg aatattccga tcacgtcgta tccttgttcc tttaacaaaa
    38461 gtgctgttac agatgagtcg acaccgccgc tcatccctac gacaacgcgt gttttgctgt
    38521 tgtctgtcat gatatcacca tcatttcaat tagattctga aacatctacg agttgaaggt
    38581 cgcaactttt cagtatttta cattatacgt catatttctg aaaatttcat cccttaatta
    38641 tcaagtcgta aaaatactgt tctactggac ctattcagta cacaaacatg atagtcaacc
    38701 aaaaaatcac tcatcttttg agcattcgat ataaaaaatt ggcatactca taaaaagggc
    38761 cagcctttcg gcaataagga ataattttat aaattgtgaa aaactatttt tcaaaattgc
    38821 ttcttatcgc ttaaagctgc acgtctcatt tacaaccttt tctttttaga tgttatgagt
    38881 tgctcagata agtgattttg tttactctaa gaaaaaaggg aggcaaaaga tacatgaatt
    38941 actttatcag tgatatgcac tttttccatg aacacttatt aggaaaaaac gactttgctc
    39001 cgcggctctt tgcaaatgtg gatgaaatga accaacagtt gatcactagc tggaatgcag
    39061 taataaaaga aaccgaccat gtttatcatc taggcgacct tgctatgcat ccgcaatatg
    39121 aaaaagcaaa tgcagaaatt ttggaactgg tcactcaact gcaaggaaca atccacttga
    39181 tcaaagggaa ccatgacagt cgcgcttttt tccgttattt agaaaaaaat gatcctaggc
    39241 ttactgacgg aagtcccaaa ttttattttt atgatgtagg tgccatcgtt aaattcgacc
    39301 atcaacaata ttacttgact cattatccga tgttgctcgg gcctaacgac actatccgca
    39361 atcttcatgg acatatccat cattacagcg tacctatcga gaacgatgtc aatgtaggcg
    39421 tagacgcacc ggaaagagaa ctcttaaagg aaaaactacc atttggcact ccgttatctg
    39481 agcaacaaat caaagaaatc tatgaagcaa agaggaagta cgataggtcg tgaaaaaact
    39541 gccctatatc aagtattaaa caaataaaga taattactca tatttttcat ttgagtaggc
    39601 ttattgccac aggaacatta ggatcagaaa tggagttgta acaagacttc tgtcacaact
    39661 ccattccata ctattcgtct ttttgctcat ggtgttcttt taccagtttt ttctgtaatt
    39721 ctttatcata atttcgggca gctgacgtta aaaataaaac gattccgcat aataaaatcc
    39781 cgattccgga aaacaagaac attttttcta cccctaaacg atcagctaat ggtcctgcaa
    39841 ataataatcc aattggacct gccaggttca tcaaagagtt gaagttccct aagacacgac
    39901 ctaaaacatt cgattcataa ctctgctgga tcattgccat cagcagtgta ttgaaatatg
    39961 gcgttgcaca acctgctcct gcattcaaga taagaaagta caagaaccct tgactatcgt
    40021 ttggaaccaa tccgcttgca ccgatcgtca ggcccataat gatgtaggct ataataatag
    40081 gtttcattcg gtctttccat gttcctataa aactgatcag tgcaccgcct aagagcattc
    40141 cgactgcgta gactacttcc actgctcccg cctgtccaac ggaaccacca aagtaatcta
    40201 gcgtcatcaa tggatacaga ctgattgctg gcatgaacaa cagcatcaca aatgcaccat
    40261 tgatcgttat gtaccataac cctttattat ccatcaattg ctgcagccca aattttgcat
    40321 ctttaaaaat agtaagcgga acttccaata tttcaggaga aacttttggg atcttcacaa
    40381 aaatcaataa gccgacacca aaaactgccc ccaatacatc taataaaata aggtaattga
    40441 caggaacgac tgaaaacaaa gccgcaccta gagctggcgc aatgatgaaa ttcgctgatt
    40501 ggaccattcc taactgtcca ttcgtccttg ttatgtgaga agatggaacg attgtcggca
    40561 taatcgactg gatcgttggc atttggaatg tttgtgcgac cgaacgcata aacaaagaca
    40621 caaaaactag ccaaatcggg aaggattctg aaatcgttcc gactactgct aaaataagcg
    40681 cgaaaatcgc tacaataata tccgtgacaa tcaaaagagc ttttttatcc cagcgatcca
    40741 ctagcggtcc tacaaatggg cttagtaaaa ccatgggaat cattcctagt aaagtagcaa
    40801 aactcaaaat tgtcgccgaa ccggtttctc ttgtcaaata ccagatgatc gcatattgga
    40861 cgaccatact agtgattcct gacaaaaact gtcccgataa aaagaacaaa taatttctct
    40921 tccaattcgt taaaacgacc gactgttcat tttccataaa catccctctt ttctttttca
    40981 ttttgtatag cgtttgcaca acaatcatta tacgggattc ttttttcaaa aaataccttt
    41041 ttccctaaat aacaatcgtt tcaatttaaa tttctttact tagaaacaat aaatcagcaa
    41101 aatattaaaa ataacaacat gtgttaaatt taaaaataaa aaagacaact aatttgcgtc
    41161 gtccttacaa agttagtcat ctaacaatag gccgtttcaa aactagttgt ctttttatat
    41221 ccacagatca ttcgactttt tcgaataacc cacggctaat catcccatcc atcagaaaat
    41281 aaaatttatc ttggctcatt ttctgttttg gatctttgaa aatattgact gctttcaatc
    41341 catttgctgt agtgatcgac attttcaatg tttgaatatc ttttgataca gtgattttta
    41401 atttgaatcc ttctttgctt tgcggcgtgg catctaatgg tcggatcaat tgataattgc
    41461 ctccgtttgt ttctgtaaat ccattatcgc gcaatgtata tctttttgca tcaggatgca
    41521 aacgatagct aattggtgcg cccagtaccg ttgctgttgt ttcaaatgcc atgattttca
    41581 cctcgataaa attagtttct ctttattata aaagtttatc ctgattttta ctaggttttt
    41641 cagttaagtt ccctaaaaaa tgaaggcgtt ctctcagaca tctgttcata taagaatgac
    41701 ttcactagtt attttttgac agcacgaact aattgatcaa ggaaatactg gatgtcgtct
    41761 tcggtatttc ccaaaccaaa gctgatacgg atggattctt ttagtatcgg tgtatcttct
    41821 ccatacattg cttctaatac gtgtgaaggc tcgatatttc ctgctgtaca agctgaacca
    41881 gtagaaatag cgaccccatt aagatcaagc ttcatcagta gcaaatcatt tgcttcgcca
    41941 attaagcgaa gattcaacac atgagggagt ttattcgtta aatcaccatt tacttgataa
    42001 gggatttcgt ttgcttctaa tgtatcaaga atcaattgct ggaatccttt atatttgttg
    42061 ttacgtgctt cacgttcttc aggtgacagc agttcaactg cttttgccat accccagatc
    42121 cctgctaaat tttctgtacc tgcacgccgc ttttcttcct gctccccgcc taaaagcaat
    42181 gcagggatat ttactccgtc tcgtttatat aagaaaccga cgccttttgg cccattgatt
    42241 ttatgagcag agatacttaa taaatcgatt cctaattctt ctggatggat cgtttggttt
    42301 ccgtacgctt gtaccgcatc tgtatggaaa taagctggat gatccgctaa aagttcaccg
    42361 atttctttga ttggcatcaa atttccgatt tcattgttcc cgtacatcac agagaccaag
    42421 atcgtatctt ctcgtaacgc ttttttgacc tcttcgatcg acagattccc tttttcatct
    42481 acaggcagat atgtaacgtc aaaccccttt gtctctaaat atttcatggt attaagaaca
    42541 gcaggatgtt cgatagctgt agtgattaga tgtctgcctt ctttttgacg cgagaacgct
    42601 gtttctaaga tagctgtatt atccccttct gttcccccac tgttaaagat gatctcgtga
    42661 ggttttgctt gcagactgtc tgcaatgatt tgtcttgctg tttccaattt ttcatgcgcg
    42721 tggcgaccaa atccatgtat gcttgaagga tttccaaacg tgtcttgcat gattgccgtc
    42781 atcgcttcaa ttacttgtgg atgcatgggg gtagttgccg catgatccag ataaatagtt
    42841 ttcacttcat tcatcccttc caaattattg ctaggcacat tctagcataa aatagcgaac
    42901 aaaccaaaga agagctcatt tttgtctaga ggacgtttca tgggcataga aaaaaggagc
    42961 tgtgacaaaa gtcttgtcgc agctccttga agctgctcac ttctgtcaca acttctttcc
    43021 ctcttttttt tgtttattcg aattcttttt cttccagacg gaataacgga ctcatcggtg
    43081 tattttcatg gatgcgtttg accgcttcac ccattaattc tgaacatgta acgattgtta
    43141 agttttctgg atgacgattt tcttctgtga aaacagaatc agtgatacaa atatctttga
    43201 ttggtgcatt gtctaaaata cttttcgcat gatcggacaa taatccatgt gacgcacagg
    43261 catatacttc ttttgcgccg ttttctttta atacatttgc tgctcgtgca agcgttgctc
    43321 ctgtgttcaa gatatcatct accatgatac atgtctttcc ttgaacatca ccgatcacat
    43381 aaccaccctc agttgaagca tcatctgcat gatctacgat ggctaatgtg ctgttcaaat
    43441 attctgaaag actacgtgca cgctgaacac cgctgttttt tggcgaaaca actacgatgt
    43501 catctcccgt cagacctaat tgacggtaat aatgagcaaa caacggcatc gtaaataaat
    43561 tatctacagg aatatcaaag aatccttgta cttgaaccgt gtgcagatct aaagtcagta
    43621 ctcgagttgc ccctgcttct tcgatcaagt tagcgatcaa tttcgcagtg atcggttcat
    43681 gaggtttcgc cgtgcggtct tgcctagcat acccatagta aggcaatacg acattgatcg
    43741 ttttcgcact tgctcgcttc attgcatcga tcatgatcag tagttccata tagtaatcat
    43801 tgacaggtgc attcgttgat tgaacgatat acacatgatc ccctcgaata ctttcttcga
    43861 tattgatgga aatctcgcca tcactaaatt gcttcacgac gcttttacct aattctgttc
    43921 cgaacacttt cgcgattttt tcagctaatg gacggttgcc attcaaacta aaaatcttca
    43981 acgtgtcatc ctgatactta ttactcatgt agtcctcccc aaaacttctc tattcttcga
    44041 tagtttaaca tatttttcca agattttctg ttaatttcag caaattctta aggaagtgaa
    44101 actgttccgt tactccaact ggcaggaatg actttatgat aatcgcccca gttgtcgtgt
    44161 actttgtaat aacgttctcc tgtttcagtt tcaaaataag cgtacgctgt cacccaatga
    44221 ttgccatatg tactgccaag tactttcaaa ataccaatca tcacaggttt gccttcacgt
    44281 atacgttttg tagctcgctg ccatgaacct agaagagttg ctcgtgcacg ctcatgattg
    44341 ccattcttct ttaaaaaact agaaatccct gtactaacct gaaaaggaat cgtaggaagt
    44401 cctagtggtt ggatctggct acgcaatgcc ttcgtcaaaa ccaacgaatc agccgtattt
    44461 ttcttgcgaa tctcatttgg aagcatccac cccttacgat agtcttgata ataggcaagg
    44521 agaactgctg ccgcgtaagt accacaaaga tagcctgagg gggtagccca tttcttatac
    44581 ctcaaaaaac gttcttctgg caatcccacc cagcaatttg cgagttcctt tcctgacctt
    44641 ttttcttcga tcaaaacggg aatatcttct tttctgttga tccatctaga taactcgatt
    44701 ggctcttcat tgatcagctt ccatttttta caagaaagac cctcttgata agtatatttt
    44761 gctggagaat attctaccgg atgtcctgat tgatcgataa tcacataact ttgatcttca
    44821 aaaaccagag ctaaaaaaag caatttactt tttcttgact gcagctgctg agtgctggct
    44881 aattttttaa tttttttatc tactttgaaa tataaaggtt tatccactac ttgattcagt
    44941 tgggatcgcc tccttttttt cattataagt gaaggattga aaaaaacaaa tcagtttgat
    45001 tgttcgaatt ttcaattttt tatgttctat ttgttaattc cttcattttg taagataatg
    45061 ggcacacgaa acaaaaaagg agcgatcgat gaatgaacaa agaacgattg aaacaagatt
    45121 tctatgaagc agtcaatgaa gaatggcaaa aaacagcaaa aatccctgca gataaaccag
    45181 ctacaggcgg tttccaagat ttagttgacg ggatcgacca acttttgatg ggagaaacag
    45241 acgatctgct tgcccatcca gaaaaagtgt cttcagaaaa aatgaaacat ttccttgcat
    45301 attatcaact ggcaaacgat tatgaccgaa gaaatgagtt gcatgctcaa ccgcttcaac
    45361 ctataatcga aaaagttgaa tcattgcagt ctttcgacga attgaataag caatttccag
    45421 aatggctgtt ggatacatta ccattgccat ttaatctgga tgtagatgca gatatgaaga
    45481 atgcgcaaac gaacgccttc tttgcttatc cgccttcttt attccttcca gataagacgt
    45541 attatgcatc agaccatccc aacggtgcac aactaatagg cgttttcttt gatatggctg
    45601 ttcagttatt agagcttgcc ggcaaagaaa aaagtcaggc agaagcaatc acggaacaag
    45661 cagtgcaatt tgataaattg attgctcctc atgtaaaaag tgcagaagaa aacgcagatt
    45721 acagcaaaat gtataatccg caatcttttg atacatttat taaatacaca gataaactgg
    45781 acttgaaaaa acttgtcacg ggtttgattg ggacaactcc agataaagtg atcgtaactg
    45841 atccagtgta cttcgatcat ttgaatgaca tcctgacaga agctcatttt ccgttattca
    45901 aaagctggat gatcattaaa acagtacgtt ctcttagtgg ttatctatca gaagaattcc
    45961 gccaagtcag cggaatctat tctcgcgtat tgtctggaac agacgaagca atgcctcaga
    46021 aaaaagcagc gtattacctt gcctctggac aattcgatca agtagttggc gattactacg
    46081 gaaaaaaata ttttggcgaa gctgctaaaa aagacgtaga acaaatggtg aaaaaaatga
    46141 tcgccgttta taaaaaacgt ctagaagaaa atgactggct tggggatgca acaaaagaaa
    46201 aagccgtcat aaaattaaat aacttaggta tccaagtagg ctaccccgat aagatcccag
    46261 cattattcga ccagttcaaa acagtacctg cagatcaggg tggtactttg ctttcaaata
    46321 ctcttgtttt cagcaaactc acattaaaag atcgtttcag caaatggaac aaaccagtgg
    46381 atcgttcaga atgggaaatg agtgctgaca cggtcaatgc ttattatcat ccatttcgga
    46441 atgtgatcgt ctttccggca gcgatccttc aagcaccatt ttacagtcta gaacaatcaa
    46501 gctctgctaa ttatggtgga atcggagctg ttatcgctca tgagatttcc catgcatttg
    46561 ataataacgg cgctttattt gacgaatatg gcaacttgaa caactggtgg acagaagaag
    46621 atcttacaca atttcaaaac aaagcacaag caatgatcga agaattcgat ggtctgcctt
    46681 ttgctgacgg aaaagtaaac ggaaaattaa ccgtttcgga aaatattgct gatgcaggcg
    46741 gtctaagctg tgcattggaa gcagcaaaaa cggaatcaga tctttctatc gaggaattct
    46801 ttgtcaattg ggcaacgatt tggcgtacaa aagcaaaaaa agaataccaa caacttttac
    46861 tgcaaatcga tgtccatgcc cctgctaaac tccgagcaaa cgtgcaacca aaaaaccttg
    46921 acgaattcta tgaaacattt ggtatcacgg aacaagacgc tatgtatcta gcgccagaaa
    46981 aaagagtgcg aatctggtaa gaggttgtgc cagaagtggt cagcttcaag aaataagaag
    47041 gaatttggga aaatcgcttc tcaaattttt gtgacatttc ggcttatttt tcgaagaagt
    47101 tacttctgga ataccatgta ttagaggttg tgaaaaagct gcttagtttc aagtagtaag
    47161 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttac taccgagaaa
    47221 ctggcttttg aacaccgttt atcgagaggt tgtgaaatca gcgtcctgac ctgagtatta
    47281 gagaaaaaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta ataccgaggg
    47341 tcagctgatt gaacaccgtt tagtggagag attgtgaaaa agctgtcctg catcaagtaa
    47401 taagaacagc caatcaaaag cagcaaggag aacctcatct ataaaaagat agaaggaagt
    47461 gagacatcta ttggtctcat ttccttctat ttttactttc ctcagtcaat tgttttacat
    47521 cataaaagga gaaattccac ttattttttt cgctgtttca tgacataaac aactacaccg
    47581 attaacaaaa ttcctccagc taatgctagc aacgggttat taaattctcc cgtttttggg
    47641 agtccttgag tgccattttt ttcactttct tttgctgtcc tatccggatg agaaggtttt
    47701 ccatttggtt ttccatttgg tgattcattt cctggatttt ctctttctgg tttgtcattt
    47761 cccgggggca catcggcagt ttctcgcaca gtgatcgctt tttgctgggt aacagattct
    47821 gtatgagcag accatttttc cccatcaaat atttccgaac gataagtgac ttctagcgta
    47881 tattctcctt ttgaaggtgc tgaaaaaata gcggtatgat tagaaaattg agcacttgac
    47941 tgtgtttcaa cgatttggat cttttctggg aaataacgca tatcttcaaa gacaggcgtt
    48001 ccttgatttg gtgtgaaagt tgacacaaaa actgtaaaag gcttcagttc aatgatattt
    48061 ccttctgcat tctccgccac accatttgct tcttgtataa taatcggatt attttgttca
    48121 tttggttttg tctgatttgg ttcttgtacg attgcatact ctggatattc cgaagacggt
    48181 gaggtttctc cgtcaaaaat agcacctaca gataatggaa gaccatccac tctttccttt
    48241 tctgtttgac gattccccgg tttaatctgc atcagtccat gaacatggat acttccattt
    48301 tcatagcgcg taacaggctg ctgaccttct ttcgaacatg tatttagaga ctcgatccaa
    48361 tcttttgata tggtatgtcc aagagtattt tctgattgtt tctctcgttc attccaatag
    48421 tagttcaacg agtggtagat ttgatcttcg ttttgattca atgggcggat ctgctctctc
    48481 atctcaagat tctctgttcg gaaagataag ataccagaag catggtagtc tgtgtttcct
    48541 gctgaagagg tataaagagc cacattatat gaaccgttat taaaacttgt actgctattg
    48601 acgataatat ctgttccgct attggaatcg attcctttcg caagattttc gaaagacaaa
    48661 ctattgatca attcatgagg gcccgttaaa gaagaaccgc ccattttgaa tccattaccg
    48721 ttaccttctc cctcaattcc tggtacccag ccgtttcgat aagcaactga gtttttgatg
    48781 attacttttc cgatcgaacc tgtttcattt ttagcaaaaa ggtcccaacc gtcatctgca
    48841 ttatgatacg cgatacaacc gtcaaataca tttccttctc ctattgtcag tttggccgca
    48901 aatccatcgg catcttcaaa tcctggatcg gcattttcaa aagatgtaca gttcttgact
    48961 aagttatgcg cgggccatgc ttcgaaagga tcatttgagc taccgctgat ttggattcct
    49021 gtatttccat tccgataagc atgaatctct tcgatcaaat tatgatttcc agaaatctgt
    49081 aatccttttt gcatagctgc actatttgta atgtcaaacc ctcgtaaacc ccagtagtga
    49141 ctgaacagtg tcatcccatt tccagttcct ttaaaatcaa gtacaggacg cttgttttct
    49201 ggatttttct ctgcaatcaa ctgaatcggg ttttctcttg tcccgttaac gccttttgga
    49261 atcgtcaatc ctctttcgaa ggaataagtt ccaggcgcca tgacgattgt ctgcccagca
    49321 tatgcgtatc tcaatgcttg tgtcagagat aatggatttt gtctgcttcc gtttcctgca
    49381 tctgaccctt ctggtgttac ataaactgtc gaatatttag gtttacggta gtccactgta
    49441 tgaagaatct cttttgtttc ataactagtc atctctacgt attcttctgg cggatagttt
    49501 ttatctggcg taaaactgaa agtaaattcg ttcgttcctt ccatcagttt tgtctgataa
    49561 tcaaattccc gattcgcagt gatagccaag ttttttgcgt tcttaagcac ctctcctgtt
    49621 gctttatttt taattgtcaa taatccgtca gcatttgctc taaaggacat agtatgatct
    49681 tggtttcctg aatgattact agaggtcaca gtgtaaatcg gatcaatcaa gcgagtaggt
    49741 ctttcttgcc caggaggatc tgtcgcagga tctgagtaag caacttcgat tccttctacc
    49801 ttgatcgtca tattccttgc tgcagcaaat cctgcataaa taaccgattc atctgaagcg
    49861 tataattttt cccagtcata catgatgtca gaagccattt cttctcctgt ttcaggatcg
    49921 taataatatg cctctaaacc agtattcgtt tttttcatct ctacgtccag tttagtaatc
    49981 gagttcgtgg ccggaaacag attcccatga ccgtttccga atatattata agaacctgtt
    50041 tctttttcca agaaccctgc tgaagtctct aaaggtgttg tttccactga gacactccca
    50101 ggagctggcg cagcttgcgg atcttcagat gatattcctg tgattgcccg tgtccccaat
    50161 ccaagtcgca tcgtatattt gctgccgctc gtgttgacca cttcttgtct atccgagtcc
    50221 cagacatatt cgatccgact gcctagtatg gcaaatgaat ttgaataaaa tgcctgccca
    50281 aacaaatgat tttttggtac tgcgtctctt accattaaag caaatccttc ttgggcattc
    50341 gtataggtcc aagattctat ttcgatcgtc gcccgcatcc taaagttgat gtctttgggg
    50401 attgcttgat aataataggt cattccatct gatccgcttg actggaattt tccgccgtta
    50461 ttcgtggaag agagccgcac gccgtcttct aatggttcaa tggcatacct actagcactt
    50521 gtgctttctc cgaaaacaac ccctttccac tcttcatttg cttcttcaga ttgctttttt
    50581 gtcttttcat tttctgattc tttcatcata ggatcatttc cttcttccgt atccatatct
    50641 tcactagttc ttggtatttc ctccattgtt gacatccctt caatgttcac gtgtgcattt
    50701 tcttcgttca cagactgttg ttcgttagca gcaacttcaa cgacagaaat taaaaccggg
    50761 gtactgagca taaggagatt catcattagc gcactgtatt ttatacgttt ttttaacaag
    50821 tgtattcctc ccgtaacaaa ttagaaagcg tttacttaaa gtattaccta atttattttt
    50881 actgtcaata aaaaacaaaa ttataataag tgtattttat aaaaagaaaa aatataactt
    50941 aagttgttga taataaagag atttattaac gtaaaaagaa taagtttttt taaaataacg
    51001 tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat tattttgtgc
    51061 tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag tgtggactca
    51121 aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc atctttgaaa
    51181 atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac acagatatct
    51241 gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga
    51301 tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt ttcctcttgc
    51361 tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc cgttcctatc
    51421 acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg atcatgcgca
    51481 tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg agcagtcagc
    51541 tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa
    51601 tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat acgagatgtt
    51661 tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa
    51721 agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc aaaaatggga
    51781 tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa gctaatgctt
    51841 cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat gtatcacctg
    51901 taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta accatttcac
    51961 tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt cctgggttgt
    52021 agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata acgatgtcac
    52081 ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc atgttcacgc
    52141 cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct ataccggaac
    52201 gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt
    52261 cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct tccattgcgc
    52321 cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg attgctaaaa
    52381 aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa gagttccatt
    52441 tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc tttttgcttt
    52501 ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt tctgcttttc
    52561 gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg
    52621 taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc aatagttccc
    52681 agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt
    52741 gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct tgtgctaaag
    52801 gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca tttgcaaaac
    52861 ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag ccagtttctt
    52921 cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga tcgatttttc
    52981 cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg acaagaagca
    53041 gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat accagttctc
    53101 tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca ataatttttc
    53161 cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg ttcagcatat
    53221 cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat ttttctcaaa
    53281 aactccttat tagtttacca tagataaacc ttagattttc tctaaatata gaaaatcctt
    53341 ctttagtccg atactcttta tagtagtccg tatgctaaaa taagtatgta atcgaataac
    53401 gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat aaaacgaaat
    53461 tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa tagtttttct
    53521 tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact ctttggttcg
    53581 cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact tgaaactcac
    53641 tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa
    53701 acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa acttaaagca
    53761 atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga attggtgaaa
    53821 gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc gaatctagtt
    53881 gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa acagacctat
    53941 gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat cgcacaagat
    54001 tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc tgttgcgaaa
    54061 cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact cttttgttca
    54121 taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt tttctaaagt
    54181 agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt tatggatcat
    54241 aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga tttattgaac
    54301 aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga gattgatcag
    54361 ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga acgccaagaa
    54421 caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt catcagctac
    54481 ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa
    54541 gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg tctccaagaa
    54601 gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt cttcgggaag
    54661 gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc acaaattgac
    54721 aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa tctgatgtta
    54781 gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta tatcgctgct
    54841 ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat gaaaagagca
    54901 gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca attcctagat
    54961 cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat ccagcaagca
    55021 ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat ccaagcatct
    55081 atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt
    55141 caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga cttattgaag
    55201 aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga
    55261 atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac aatccaagag
    55321 acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga
    55381 cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat cacctttcgc
    55441 ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg cgtttttctt
    55501 atctttcttc taacttctcc attggagtct atttctcatt catcttgctg tgtccatagc
    55561 catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg atccatgtca
    55621 cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt ggcaatagcg
    55681 gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt ctcaacttca
    55741 gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt agttccgcta
    55801 atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg gcataagtcg
    55861 cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga agtaagccat
    55921 ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc gttaccgtca
    55981 gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt cctacatacc
    56041 tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg attcctgtca
    56101 agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca atgattcctc
    56161 ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg gcatcaaatc
    56221 ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa ccaaaaggca
    56281 tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata aacaacgcga
    56341 tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc ttggcttcaa
    56401 gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca atgtatgtac
    56461 cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct aaacttttca
    56521 aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt
    56581 ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca acatacagat
    56641 aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa cttagtccag
    56701 acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga ccagcatttt
    56761 gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca ccaattccaa
    56821 gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga ttgagattga
    56881 tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg gaaaacagcg
    56941 attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa aggcaaccaa
    57001 caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta
    57061 aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt
    57121 tgactgttag aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt
    57181 tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca
    57241 ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga
    57301 tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat
    57361 caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg
    57421 caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc
    57481 attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca
    57541 atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa
    57601 cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac
    57661 taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt
    57721 gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca
    57781 tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa
    57841 acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa
    57901 cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct
    57961 tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa
    58021 agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta
    58081 tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg
    58141 ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat
    58201 ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa
    58261 acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc
    58321 tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc
    58381 cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt
    58441 gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga
    58501 actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa
    58561 tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc
    58621 agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa
    58681 ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc
    58741 aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca
    58801 attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca
    58861 ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc
    58921 atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat
    58981 atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg
    59041 tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta
    59101 agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat
    59161 caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt
    59221 ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga
    59281 tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt
    59341 tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt
    59401 tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat
    59461 ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg
    59521 tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga
    59581 tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg
    59641 ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt
    59701 tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca
    59761 gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt
    59821 gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata
    59881 tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg
    59941 aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca
    60001 cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg
    60061 tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac
    60121 catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt
    60181 ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg
    60241 gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat
    60301 caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt
    60361 cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga
    60421 cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca
    60481 ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct
    60541 cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa
    60601 ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg
    60661 tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg
    60721 cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc
    60781 attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag
    60841 aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact
    60901 ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata gcttccataa
    60961 gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc
    61021 atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt
    61081 ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct
    61141 gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct
    61201 tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt
    61261 tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca
    61321 aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct
    61381 agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct
    61441 aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca
    61501 aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg
    61561 gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt
    61621 ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata
    61681 ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt
    61741 tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc
    61801 atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat
    61861 gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc
    61921 atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag
    61981 aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat
    62041 accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa
    62101 aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg
    62161 tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg
    62221 tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat
    62281 ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc
    62341 acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc
    62401 cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc
    62461 atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc
    62521 tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga
    62581 ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt
    62641 ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc
    62701 taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac
    62761 ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc
    62821 cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt
    62881 aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt
    62941 gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata
    63001 tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt
    63061 ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac
    63121 atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat
    63181 tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc
    63241 tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg
    63301 tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac
    63361 cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt
    63421 cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc
    63481 atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg
    63541 tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg
    63601 gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc
    63661 cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag
    63721 cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc
    63781 tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat
    63841 gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca
    63901 ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct
    63961 aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact
    64021 tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct
    64081 cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc
    64141 acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg
    64201 tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc
    64261 tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt
    64321 tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt
    64381 tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac
    64441 ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt
    64501 gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt
    64561 aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa
    64621 cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa
    64681 gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct
    64741 aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat
    64801 aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc
    64861 ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca
    64921 tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg
    64981 gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact
    65041 ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc
    65101 cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt
    65161 tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta
    65221 ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc
    65281 agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa
    65341 agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact
    65401 ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg
    65461 gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt
    65521 ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc
    65581 ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt
    65641 tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc
    65701 tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg
    65761 gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc
    65821 tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt
    65881 tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta
    65941 taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct
    66001 tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct
    66061 ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta
    66121 aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata
    66181 tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg
    66241 ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt
    66301 tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc
    66361 tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt
    66421 tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga
    66481 gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct
    66541 tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta
    66601 ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct
    66661 tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc
    66721 ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt
    66781 ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta
    66841 agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga
    66901 ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct
    66961 gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc
    67021 agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg
    67081 gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt
    67141 ggactgattt cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt
    67201 gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt
    67261 ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa
    67321 agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta
    67381 gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc
    67441 aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc
    67501 caatttccta acacttttgt atagtcattc gttgtaaact tcatctattc tttcatcaac
    67561 caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat
    67621 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
    67681 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
    67741 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
    67801 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
    67861 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
    67921 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
    67981 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
    68041 cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa caatgcccat
    68101 ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt tagttaaaaa
    68161 acaccaatta tacaacattt attattaaaa ttaaattata aaattttata acgttctcat
    68221 ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc tcgaaacaaa
    68281 aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat taattcagat
    68341 aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta aaatgtcagt
    68401 aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc
    68461 taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa aggctagcgc
    68521 tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga caagccgacg
    68581 atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa
    68641 aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat caggtatcaa
    68701 gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat tactgataaa
    68761 tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact tgttgataat
    68821 actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta agaacctttt
    68881 cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg aaacctaata
    68941 aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca
    69001 aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta atccaagaag
    69061 cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg cttaatagaa
    69121 gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa tagcggaaaa
    69181 tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct tgctttccat
    69241 aaaaagagaa gttctcacga aactttttcc agaatagtga accgagcttt ttctcttcat
    69301 tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct aatgtgaaaa
    69361 caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga atccattcaa
    69421 tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc atccttttga
    69481 acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat
    69541 cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg tcgttgcttg
    69601 gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt ggatatataa
    69661 cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa
    69721 ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat tcgctccgtc
    69781 gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac gcatcagaaa
    69841 tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag tacctaccca
    69901 gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg ggatcatcat
    69961 cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct tcgcaaatcc
    70021 ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag tgatgaataa
    70081 tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat agttttccca
    70141 tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca ttggtttcaa
    70201 tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta ctgtcatgag
    70261 gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga aataatccat
    70321 catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac
    70381 gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata acccatatca
    70441 aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga atttgcaggt
    70501 ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa agaaccgatc
    70561 gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt aatacttttg
    70621 aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga tctaggaaca
    70681 ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat tttcatcaaa
    70741 atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg gatatggaac
    70801 cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat ccataagatg
    70861 tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa tacactgatt
    70921 cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt
    70981 acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa atcatcgtga
    71041 acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt caaattataa
    71101 tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac caagaacccg
    71161 ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt tcggatagat
    71221 aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg attattgatt
    71281 ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt ttggtgattt
    71341 tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat ccggagcatc
    71401 taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt gatagatcat
    71461 ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg tcttgctcgc
    71521 catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag aaagatattt
    71581 gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca agacgaatcc
    71641 gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca tcacgccaaa
    71701 atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat tttgggcacg
    71761 catggctact aatccggaaa taaatggatc agatacacca gacccaaatt cggcttctaa
    71821 tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact ccaaggcttc
    71881 cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt
    71941 atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa aaccgatgcg
    72001 tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta attcatccca
    72061 agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt tattataaaa
    72121 aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat attctactgt
    72181 atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt tggagagatt
    72241 agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta caataacatc
    72301 gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac cccatggctg
    72361 atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa tgatttcttc
    72421 gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat tttttcttcc
    72481 atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg cattacttga
    72541 tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa acaataattt
    72601 tttcatattt tacctccaaa ttttaatgta accgatgccg gttttaatat aacgatatca
    72661 ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca
    72721 atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt agcagaatga
    72781 gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag
    72841 tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc
    72901 ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc
    72961 cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac
    73021 gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa
    73081 gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag
    73141 cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc
    73201 ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc
    73261 gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta
    73321 aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt
    73381 gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt
    73441 gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt
    73501 agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg
    73561 aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat
    73621 gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac
    73681 acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt
    73741 tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat
    73801 tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac
    73861 tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact
    73921 atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt
    73981 tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc
    74041 acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc
    74101 tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat
    74161 ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct
    74221 caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg
    74281 ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat
    74341 tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaacaagaa
    74401 cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact agccaacaat
    74461 atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc
    74521 atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa
    74581 ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt
    74641 gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt
    74701 gacctccaat ttttaactct tctatctccc atccagtata cataaccttg atacttcgac
    74761 cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg
    74821 ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac
    74881 tattaatttt taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac
    74941 tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg
    75001 ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa
    75061 aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc
    75121 aactcattac tgtatgattt tagcagccca accaacatat accctataaa agctaataag
    75181 atggttagga atcctttaga atttttactc attcatagac acttccttaa ataactttta
    75241 ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaaact
    75301 tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat
    75361 cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac
    75421 taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac
    75481 tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa
    75541 ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg
    75601 atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc
    75661 agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag
    75721 catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta
    75781 aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac
    75841 gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa
    75901 atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa
    75961 tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta
    76021 tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata
    76081 atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt
    76141 tccttaaaat caagagattt taattgtgta ataattactt gtccttttat atctagctct
    76201 tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc
    76261 gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt
    76321 tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga
    76381 atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc
    76441 attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg
    76501 gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag
    76561 gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt
    76621 ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg
    76681 caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga
    76741 tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag
    76801 aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct
    76861 tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca
    76921 tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa
    76981 agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc
    77041 aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg
    77101 agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg
    77161 ataaatggca acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa
    77221 ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa
    77281 ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga
    77341 ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt
    77401 ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta
    77461 tgttatacct tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa
    77521 gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca
    77581 tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt
    77641 tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac
    77701 ataaatttgt ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg
    77761 gttgatgaaa ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc
    77821 cttttttaaa tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct
    77881 cttttcttaa cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg
    77941 cctgcttcgg ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta
    78001 gatcaaaaaa ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac
    78061 tgcaacaaga agttcgcacc aacaaaacac tttcaatttt tgtagaacta aaaaagttgt
    78121 gacaagtgac aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca
    78181 caatattcaa caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa
    78241 acggcaggct tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc
    78301 caaatttatg caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact
    78361 aagcgacttt ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    78421 aagcccaacc aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga
    78481 tgcagggcag ctttttcaca acctctccac taaacgatgt tcaatcagct aaccttcggt
    78541 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    78601 aggtcaaagc gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga
    78661 aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    78721 cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa
    78781 caatctttct ttgaccaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt
    78841 caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc
    78901 ttcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca
    78961 ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat
    79021 aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat
    79081 ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc
    79141 gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt
    79201 ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc
    79261 tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat
    79321 cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca
    79381 gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca
    79441 cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga
    79501 ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact
    79561 tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca
    79621 ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg
    79681 gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt
    79741 ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg
    79801 atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct
    79861 tccactgatt catattgact ttttctagca aacatctgct gctcaagcat catatgttct
    79921 cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag
    79981 agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg
    80041 tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg
    80101 acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca
    80161 ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt
    80221 tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg
    80281 acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg
    80341 actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt
    80401 gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa
    80461 cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc
    80521 gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag
    80581 atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct
    80641 gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt
    80701 tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct
    80761 tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct
    80821 gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct
    80881 aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga
    80941 acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca
    81001 gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca
    81061 atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta
    81121 ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt
    81181 tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg
    81241 atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc
    81301 tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg
    81361 atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca
    81421 ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata
    81481 ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa
    81541 atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc
    81601 agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac
    81661 aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg
    81721 cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttcgaa
    81781 tactacaccg atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac
    81841 tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca
    81901 agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg
    81961 aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc
    82021 atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca
    82081 tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg
    82141 cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac
    82201 tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt
    82261 ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg
    82321 ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc
    82381 actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg
    82441 gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt
    82501 actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc
    82561 tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat
    82621 cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc
    82681 ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat
    82741 cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata
    82801 ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca
    82861 tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta
    82921 tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata
    82981 taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg
    83041 gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg
    83101 atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca
    83161 tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt
    83221 tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc
    83281 aaaaattggg aacctgttga atccgcacct aagctattca atactttttc agcaaagaat
    83341 ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg tttgctttct
    83401 agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg
    83461 tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata
    83521 aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa
    83581 taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat
    83641 tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct
    83701 tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca
    83761 tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc
    83821 cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat
    83881 ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct
    83941 ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct
    84001 gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga
    84061 caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt
    84121 cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg
    84181 ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta
    84241 ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg
    84301 ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc
    84361 caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat
    84421 tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta
    84481 ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc
    84541 aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg
    84601 tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac
    84661 gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc
    84721 atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg
    84781 gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa
    84841 aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct
    84901 tccagtggat agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac
    84961 atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga
    85021 ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc
    85081 gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc catttttctt
    85141 cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg acttggctaa
    85201 tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga
    85261 taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct
    85321 ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta
    85381 gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc
    85441 atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa
    85501 tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg
    85561 aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt
    85621 ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa
    85681 ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc
    85741 ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca
    85801 attggcccca cttatggagg cattatgact tcacatttta catggaatca tatcttcctg
    85861 tttcttgtgc ccgttttgat tctttctttg attatcggct ggtttgctat tcccgagatg
    85921 gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt
    85981 agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc
    86041 atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg
    86101 atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt
    86161 tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa
    86221 ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg
    86281 ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct
    86341 gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca
    86401 gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc
    86461 aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca
    86521 atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt
    86581 atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac attaggatca
    86641 aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat
    86701 tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt
    86761 tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta
    86821 tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg
    86881 ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc
    86941 cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt
    87001 ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga
    87061 cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa
    87121 aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag
    87181 cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg
    87241 gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc
    87301 aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag
    87361 aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag
    87421 aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat
    87481 cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc
    87541 ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt
    87601 tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg
    87661 atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt
    87721 cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg
    87781 gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct
    87841 taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc tatattacga
    87901 aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg
    87961 attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt
    88021 cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa
    88081 tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat
    88141 ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt
    88201 gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga
    88261 aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag
    88321 cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta
    88381 gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg
    88441 ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat
    88501 catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg
    88561 gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta
    88621 ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag
    88681 aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg
    88741 cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag
    88801 agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa
    88861 atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg
    88921 caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    88981 ctccaaagta acgtaatcct agcacaaccg ctgtattatg gagagcatca aacaaaatga
    89041 gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct
    89101 catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat
    89161 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    89221 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac
    89281 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    89341 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    89401 tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    89461 tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa
    89521 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    89581 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    89641 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    89701 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    89761 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    89821 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    89881 attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta
    89941 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    90001 agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc
    90061 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    90121 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    90181 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    90241 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    90301 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    90361 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    90421 gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga
    90481 aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata
    90541 tttttttgat acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg
    90601 ttttttttat ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa
    90661 aaaaaagcaa aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca
    90721 aaaaaaccag taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt
    90781 tttttatttt aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac
    90841 ttcttctgtt tctttagcta atatttcatc taatacatca agccattttt ccggttcttt
    90901 tgttcctaga cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt
    90961 taaacattct gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa
    91021 tgcgtttctc tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg
    91081 tttgaagtct ttattagaca tcgaaagcat tggctttaat tttggatata tttcgtctac
    91141 ttttggttcc atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa
    91201 ctggcagata tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat
    91261 catccctttt gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc
    91321 aagcaatgct tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc
    91381 tcctggttta tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga
    91441 accgaactct tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg
    91501 tgctacagcg acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc
    91561 ttctttttgc catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc
    91621 tctgagtata tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc
    91681 atccctaggc acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga
    91741 tttcggctga tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa
    91801 gccagaagta tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg
    91861 ttccgccgtg gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc
    91921 ttcttttaat gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag
    91981 tttttctttt ttcagctgga caaactggat ctgctgatcg gcattcttct tttcttccat
    92041 ccattttttt cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt
    92101 atctaatgca ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct
    92161 tacctcttcc catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag
    92221 atttttttgc tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac
    92281 tagacgatta taaaacatat tcattaattt gctgttggca ataaattgac ttttataata
    92341 ggccgactgg atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca
    92401 tgctttgacg gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg
    92461 agaaaggaac acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa
    92521 caaccatttg cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat
    92581 gaataattta gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc
    92641 ctgctggctg atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa
    92701 attcatagcc acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta
    92761 tctgtacgac gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct
    92821 gctcctcaag ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg
    92881 atcgtttcaa tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt
    92941 gctatggaat cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt
    93001 tccatgcttc ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt
    93061 atctgatcaa tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc
    93121 catatataag ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt
    93181 gttactaata attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct
    93241 aattctttta gtttttccac atatgcttct gcattttttt tatagagtgt cgcattttct
    93301 gcatcttttt cagagaatat acgtgcgatt tcctccacat attttattcc gttagatagg
    93361 tctagccaag catggggatc tgcttttgta tgttcttcac cgctagttaa ataaagaggt
    93421 tctacatttt tgctaactgc aaaataatct tttccttctt cttttttagc cgtttccatc
    93481 aagttatcga accagctgtt acctgtttca agattcaaac cgttgtataa aataacatct
    93541 gcatcgcttg cctttttgat gtcttctggt agtacttcat attcatgcgg atctgttccg
    93601 actggtacga tactgtggat atctatttga tctgttccga cctcttttgc catgtccgcc
    93661 aagatcgaat tggtagccac gactgacaac ttttctttac ttccttcatc cgcttggtca
    93721 gaagcctgct ttccgcaacc ggctaatacc aagaaactaa ctgccaagat taaaaagagt
    93781 gatttttttg ttttcatcta tctgcttcct ttctgaaata aaattccttt tgttggagaa
    93841 aataaaaaag caaggataaa aaatgcggta catgctaaca cgatcgtagc tcctgatggc
    93901 caattgtaac tataactgaa aaacacaccg acaactgcac ttagcatacc gattcctgca
    93961 gctgtaatga tcattttcag taaatggttc gttagcaagt aagccgtggc ggctggtgtg
    94021 atcaacatgg caatcaccaa gattgttcct actgtctgta aactgactac agccactaat
    94081 gtcaaaaaga acatcaaaag ataatggatc agcgatgtgt tcagcccata agcttttgcc
    94141 attgtcggat cgaatgatgt gatcttcaat tctttataaa agaaaaagac aaatatcaag
    94201 acgattccac taacgagtgc tgtcagcaac aaatcgcttt ccctaacagc taagacattc
    94261 ccaaataaaa tatgatacaa atctgtcgca ctctgggcat atgaaatcaa gataatccct
    94321 aatgcaaaaa aggagctaaa gacaattcca atcgctgtat catttttcaa tcggctccgc
    94381 tgagtcacga aaccaatcac tgctgctgtt agcatcccaa aagcagtggc tccaaagatg
    94441 taactaaaac caaacatata agaaatggct acacctggaa gaactgcgtg agagatagcg
    94501 tctcccatca aagacatccc tcgtaaaata ataaaagaac cgatgactcc agaagcaagt
    94561 cctacaagca tggaagtgac caaagcgtat tgcaggaatt gataattcat taatccatca
    94621 ataaaggatt ggatcatttt ttctctcctc cttttactaa atgtctgatg atctcaccat
    94681 aggccaattg aaggttcttc gatgtaaaga cttcttgtac tggaccggaa gcgatcagct
    94741 gtttatttaa aagaataacc tcatcgaaat attcttctac tttatgaaga tcatgatgga
    94801 cgatcaaaat cgtttttcct gaattcttca attcctgcaa gatgtcaaag atctttcgtt
    94861 cacttaacgc atcaatccct acgaatggtt catctaaaaa gatccattct gctccttggg
    94921 ctagagctct tgcaataaaa actctctgaa gctgtccacc cgatagttcg ctgatctgtc
    94981 ttttggcata ttcttccatc cctacttttt tcaaagcttg tcttgcacgt tctttttctt
    95041 ttttcccagg tctttgtcca attcgtaaag atggatatgt tcctaaaagt acaacgccta
    95101 atacatcaat tggaaaggaa agatccaatt cacttcgctg ttccacgtag gctatttttt
    95161 tacggactga gtttaccgac tgtccagaaa aggtgacttt accagtagta gctggtatca
    95221 actctagcaa agatttcata aatgttgatt ttcctgcacc gtttggtccg ataataccgg
    95281 taattttttg tggatttatc gtcaagttga tagttttcaa aacagttttc ccttgataag
    95341 cggctgaaag gtcttgaatt acaatggccg tacgttccaa atcttacact cctttttagg
    95401 tttacctaac tttttattta aatatagttt aactaaaatg gaaagcattt tcaagtagaa
    95461 tgatgaaaaa gtttttttcg aattttcctc gcattttatg aaaattttct gtagaatcac
    95521 atcaagagtt caataaggct aacctaataa tgaaaaacat gtgttgaaac gtttgattat
    95581 tttttcttgt tgactcaaca acgaaaggag tttttgtatg gaagaagaaa agttaactcg
    95641 aagtcttagt gcccgtcata tccaaatgat cgcactaggt ggaacgatcg gtgtcggtct
    95701 gtttatgggt gcctcctcaa ccatacgttg gacagggccc tcagtgatgg gagcctatgc
    95761 gattgcagga ttctttttgt atttgattat gcgtgcttta ggcgaaatgc tttacgttga
    95821 tccttcgact ggttcttttg caaattacgc gagcgagtac atccatcctg tggctggcta
    95881 cttgaccgct tggagtaata tttttcaata tattgtcgta ggaataagtg aagtgattgc
    95941 tgtaggatct tatatgaatt attggtggcc tgatttgccg gcaattattc ccggaatcat
    96001 tgtcgtcttg tttcttatgc tggcaaatct tatttctgtc aaggcgtttg gcgaattaga
    96061 attttggttt tcgatcatca aagtcataac gatcattttg atgatcattg caggtcttgg
    96121 ggtaatctta tttggatttg gtaatcatgg tcaggcagta gggatttcaa atctttggaa
    96181 aaatggtgga ttcttcactg gaggcgtcaa aggatttttc tttgcattat cgatcgttgt
    96241 tgcttcctat caaggaatcg aactgatcgg aatgactgct ggtgaagcag agaatcctaa
    96301 aaagacgatc attgaagctg tccagtccac tatcggtcga atcttgattt tttatattgg
    96361 tgctattttt atcattgtga gtatctatcc gtggaatcaa ttgagtgaga ttggttcacc
    96421 ttttgtacaa actttttcta aagtcggcat tacatttgct gcaggactga ttaattttgt
    96481 cgtgattaca gcggcattat caggttgcaa ttcagggatt ttcagcgcaa gtcgtatggt
    96541 ctatacactt gccataaatg gaaagatgtc taaaaaattt ctgaaactat ctcgtcacgg
    96601 tgcgcctttt tatcctgtcg tagcaatctc aggtggaatc ttaattggtc tgatcttaaa
    96661 ttatcttcta ccaatgttca tcaaccagtc agaaaatatt ttcgttttcg tatacagctc
    96721 aagtattttg cctggtatgg ttccttgggt agtgattttg atcagcgaga ttcgcttcaa
    96781 aaagaagcac gcagataaaa tgcatgatca tccttttaaa atgccactat ctccttatac
    96841 gaattatgtg acacttgctt ttttagctat cgtattgatt ttcatgttta tcaatcctga
    96901 aacgagaatc tcattgatta ttggactcgt tttcttgctt tacatgacga tccattactt
    96961 tgtacgcgaa aaaagaaccg gaaaataaaa aaagaacgaa gcttcacctt agtggaagct
    97021 tcgttctttt ttattgcttt tatcaattca attttagcgt ctcaccggga gtcaggaaat
    97081 aattctccat gctcatccca ttcagcgaca agatcgtttc tactggtacg ccagttcgtt
    97141 cggctatttg atttggacct tctccttcca aaactacggt tgtcgcagcc tgtgaagtcg
    97201 cgctgttggt attagtttca gaggaatctg ttgtgtcttg tgttgttcga gcttgcattt
    97261 cagaagcttc taccgactct gctgtctcag aatcagaaga tgttgtgtct tgtgtgcttg
    97321 ttgactctgc ttgaagagag gcgctagtct gcaaataaat atcggaaatc ttagcgacat
    97381 gattcgtata attagccgat aattgattga tcaaagtatc taagtctaca ccgtctttat
    97441 tttctgctgc ctgatacgct gcacgtaatt ttctgatccc ttcgtcggag attgcttgaa
    97501 gctcacgtgc tttttggtta gcaactgctg ataaaccagc tattccctct tgatttccct
    97561 gaatttctgt ctggtattct tcaatcaatg caggcgtctt ttcttcgatc tttttcgaat
    97621 aatcatttac gattgcttga agttgctctg gcaaaccatt tgctgttttt tcttcagaag
    97681 tttgtgtctc ttttgtttta gttgatgact cacttgtttg tttcgtctct gttttctttg
    97741 tcgttgtttc cgttatggtc gtacttttcg cttctgacga attttttcca tgaaaatata
    97801 gacccgcaac tcctcctgct atgatcaaaa acacggcaac tgctgcccca atcatgatca
    97861 tccgcttacg agctgcctgt ttttgctgtc ttcttcgggt catccgaaca ttttctcccc
    97921 actcattttc atcatacatt tttttcacca ccaattttta tgtataatag cgttatcaaa
    97981 cgcttcatat tcttattgta gcaaataatt gtcgctgtgc acatttttat ctttgttatt
    98041 tataagaaaa tttcaccagt tgagatgatt ccaagattgt ccgtcgattt ctccttgcag
    98101 ctgcattgtt cgttcgtcta tctcagccat cagtttcttt atttcatata agagcgcacg
    98161 gtcttgatat tctgattttt cgcttaattc atctaattgt tggatcagcc aatcatttgt
    98221 cataatgaag ttcctttcat ttcttgcagt gtaaaggtca agtcttcgat ctgctctttt
    98281 gcctcagtca gtgcttctac gatatatgct tgtttttcag attcttcttc ctgcattttc
    98341 tgattttcct gtactctttg aataaaccag tcaatccgct gagtcagcga tggtttgcct
    98401 tccaatgaga gatactgctg ataagcaatt agcttctccc aacgtttttc ctgggataaa
    98461 tacataaatt gtgctaaaac gaatggcgga agataggtca tatggaattt atttgcgatt
    98521 cgctgatacg gcctcaaaaa ttcggaaatc gtatattctt cttttcctcc agcttggtat
    98581 tctttttccg ccacacctgt tgtcaatacg attcctagtt ctttcccttt taatacatgt
    98641 gctgctcgtt ccaagacatt atcttgccaa atttttaaat gagcaggaga actataccag
    98701 taaaacggaa actggaaaat gatccgatca tgctgggcca agagctgtag ttcagtctct
    98761 atatctattt ttccgtctgg ataacttgat tccaaatgat gccaagtagc tggtaaacca
    98821 gcagaagctt ctttgaaaaa ttgctgattt aaagattctt cgatcgttgg atggctaatg
    98881 atgataagtg tacgcatccc ttttcctcct ttttagtctt cgctttacag tgtagcataa
    98941 ccgccacaag tttcttttgc cgatgcttgt cattttaacc agactaatca ggttgtccaa
    99001 gaaactgtgg tttgtttttc caactgtttt ttcaaggtat ccaaagcaat attcttaaaa
    99061 tttttgtttt tcatacgttt acgatagttc gtagccgttt ctccggtact ctttttgaac
    99121 attcggctaa atacgactag actattgaac cctacttgat aggcaatact ttccacggat
    99181 tcatccgtca gagaaagaag aaacgcccct cgattgatcc gtaccccgat caaatattcc
    99241 ttgatactgg tacccatatg atttttgaat actcgcgaca aatggctccg attgaccgct
    99301 aaatgctggg cgatttcctc aacagacaat ttttcagcat aatgtgcctc gatgtatttg
    99361 actgtttcaa cagctaaaga ggaatgaacc ttttgtacac caccgctgac ctttccgcca
    99421 tcttttaaaa ggactgataa aaaacggtag gttaatgctg ttaactgtaa ttcattttcc
    99481 aaggtttctt ccgtatcttt catgcattct aaaataatgt cgatatattt ttgtatgtca
    99541 gaagagacca tcgtgtattg gtcttctctg aaaagcgact gccgaatgat ttcttctgca
    99601 gcactcccac caaatgaaag ccagcaatac atccacggat cttctccatc tgataagtaa
    99661 aacgtcgagt ctcctggacg aatcaagaac agatcacctt ttttcaattg gaattgttga
    99721 tttttcacat gataagatcc tttgccttcc atcacgacat gcaggatgta atcattgcga
    99781 atagcaggac caaaagaatg aaacggcatc gttttagaga aaccacaaaa atcaaaatac
    99841 aattcttttt ctacttgttg atgttccttt tttaaaaaaa tcgattgctc cacgtctatc
    99901 cctccttact cctttagtat aatgcaacaa aaagttaaat aaaagtcaca gaaatatatt
    99961 aaaaaggaag cgatttcttg ttaagtttaa gtcattaaaa gatctttgtt attcgttgta
   100021 accggtttac ttagtattat atattgaaca ggagaggaat acagattatg aaaagcaaaa
   100081 tgatccaacg tgtgtcctac gctttaggcg cttttggaca cgacacattc tatgttacat
   100141 taagcactta ttttatggta ttcgtgacaa gccagatgtt cgcaggaaca gataaggcaa
   100201 cgaatgcaaa aatgattgct acagttacaa gcttagttgt aggtatccgc ttagttgaaa
   100261 ttatttttga tcctttgatt ggtggcatca tcgataatac tcgtacaaaa tacggtaaat
   100321 tcaaaccatg gttagttatc ggcggcgttg ttagttccgt actcttggta gcaatcttta
   100381 ccaatttctt tggcttagct actcataata cgactttatt tatcatatta tttgcaatcg
   100441 ttttcatctt attggatagt ttttattcat ttaaggatat cgctttttgg tcaatgattc
   100501 cagctttgag tacggaaaca aaggaacgtg aaaaatttgc tacgtttgct cgtttcggct
   100561 cttctctagg tgcaaatggt actactctag tcgttgtccc tatcgttact ttctttacct
   100621 atcttgttac tcataaacat attgaaggcg cttcaggctg gttctggttc ggtgtgattg
   100681 ctgctgttat ttcaggcggt actgcgatta tcactgcctt gggcacaaaa gaagtcgata
   100741 ctgcgatccg tcagcaaaat gaaaaagctg ggatcaaaga tgtgttcaaa gcagttgctc
   100801 aaaatgatca gttgatgtgg cttgcccttg cttatatcgc ttatgcgatt gccaatgcag
   100861 caacaactgc cgtattattc tacttcttca aatatgtact tggccaacca actgaatttt
   100921 ggatcgttgg tctagttgct gccttacttg gtttagtagc tgttccacta ttcccaatgc
   100981 ttacacgcgt catctctcgt cgctacgttt accttggagg gatcgcaatg atgatttctt
   101041 cttacttttt ctttacgatc gcaggttcaa gtttgccaat cgttatcatc gggctagtat
   101101 tgttctactt ccctcaacaa ttgattttct tatccgcttt gatgacgatc acagactctg
   101161 ttgaatatgg tcaatggaaa aatggtatcc gtaacgaagc tgttactcta tcaattcgcc
   101221 ctttactaga taaaatcgct ggtgctttgt ctaacgggat cgtagggttt gtcgctatcg
   101281 cttctgggat gactggtaat gctactgctg ctgatatcac tgctcatggc gtaacgactt
   101341 tcaaagctta tgcattctat gctcctgctg ctttgatgat tttctccgct attatttttg
   101401 cttggaagat tacattgaca gagaaaaaac atgcacaaat cgttgagaaa ttggaaaaca
   101461 aattggctcc agctgataca atggaagatg aacttcacga tgcagtagat ccttcccatt
   101521 aagaaaggat cttgaaaatg aagattacag aaaaagattt tggccaaggt tatcacttga
   101581 taaccttgga gaacaaaaac aagctagcac tttctatctc tgatttaggt gctagaatcg
   101641 tcagtttaaa atcaaatgat cgagaattag ttttgggatt tgacaccgct gaagaatata
   101701 tcgaaaaaga tccgtatatc ggtgcttcta tcggtcgcac cgcagggaga atcgaaaatg
   101761 gccacttttc tctcaatgga aaaacttacc aattagccac agatccaaaa actggacata
   101821 atctgcatgg tggtgctcct ggatttgaat taaaaaaatg gtcttatgtc attttgaatg
   101881 gcgaaaatga agcttctgtc atttttacta ctacaagtcc cgacggagaa catggttttc
   101941 caggtacaat ggatgtggaa atccgctata ctttgacaaa agataatatc tggcgagtga
   102001 cctctcgcgg caccagtgac caagatactt tattcaatcc gacaaatcat gtttacttca
   102061 acttgacggg agatgcttct cagtcaatcg atcaacatga gctttggctc aatagcgaag
   102121 cttatgctcc tttgcgaaca gatagtattc ctataggggt aaaagaaaat gctgcaggaa
   102181 gtgctttcga ttttcaaata cctaaaaaat tagcgtccgt ttttgctagt gatttggatc
   102241 aaaaaaatct tgttgacgga atcgatcatc catttttctt aaaagaaacc ggcttgggta
   102301 aagaagccgc tcgcttgact agccccgata aaagaatcca agtagatatt gctacagatg
   102361 cttctagtgt ggttattttc actgcaaatt tcggcacaga aacaccagaa atgcgcaacc
   102421 gaaaactggc acatcacggt ggcatcacat ttgagactca aacagctcca ggtgcagagc
   102481 gcttctcagc atttggttcg attcatttaa aagccggatc agtatttgaa acagtgacag
   102541 aattcaaaat caaaacaaga aaggaataac tcatcatgga aatcaaaaga agcttaaaca
   102601 caaaatttgc cgaaacattc ggacctaaaa aaactacaca atacttctca ccaggacgca
   102661 tcaatttgat tggtgaacac acagattaca atggaggcca tgtcttcccc gcttccatca
   102721 cttatggaac gcaaggtgta gcagcacctc gagacgataa taaaatatta gtttattcca
   102781 caaactttga cgatatcggt gtcattagtt ttactttaga tgagctagaa tatgataaaa
   102841 aagcaaactg ggcaaattac gtaaaaggaa tggtccttaa attaaaagaa gctggacact
   102901 caatcgatca tgggtttgag ttattagtgg aaggaacaat ccctaatggt gcaggacttt
   102961 ccagtagcgc ttcattagaa ttattagtag gtgttgtatt agaagatctg tttgatttaa
   103021 aaacagatcg cttggaacta gtccaaacag gtaaaaaagt agaaaatgaa ttcatcggtg
   103081 tcaattcagg tatcatggat cagtttgcca tcggcttcgg tgagctagat catgcaatct
   103141 tattagatac aaatacgctg aaatacgaaa tggttccagt gaaactagat ggttatgcaa
   103201 tcgtcatcat gaatacgaat aagcggcggg aattggcaga ttcaaaatac aatgaacgcc
   103261 gtagcgaatg cgaagaagcc ttggcacgtt tgcaaaaaac tttgcctatc tcttctttag
   103321 gagacttaga tgaagaagaa tttgaatcaa ctattgacca aatcggcgat gatacattga
   103381 tacgcagagc tcgccatgcc gtttctgaaa atcagcggac tttgaaggca aaagctgaat
   103441 tagaagctgg gaacttagaa gcatttggtc aactgttaaa tgattctcac cactctcttc
   103501 gctatgatta tgaagtaact gggatcgaat tagatacact agtagatgct gctcaaaaac
   103561 aagaaggtgt cttaggtgca cgaatgacag gagctggatt tggtggctgt gcgattgctc
   103621 ttgtcaaaga agaaaatata cctgccttca aaaacaatgt ctatgatgaa tacatgaaag
   103681 ttgtcggcta tgctccggaa ttttatgttg cacacattgg aaatgggaca acaaagatcg
   103741 atccagaagc tgttcatgtt tgataaaaaa atcaacgatc tcagctaaaa acaaatagat
   103801 aaaaataacc tctcagcaat ttccaaatgt gagccttttg ctcgtatttg aaaattgctg
   103861 ttttttattg acacggaaag agaacgtgtg ttctaattgt gttgaagagg tgattgttat
   103921 gtgggcgaat caaaatccgg tatttgttta tgaaaaagaa ccttcaagag atattttatg
   103981 tattgactgc aaatcttttt acgcctccgt agagtgcgtt gaaaaggatc ttgatcccct
   104041 tacaactaaa ttagtagtca tgtcctatcc gtccgattcg acagaaacac ggggtagcgg
   104101 attaatcttg gcttccagtc ctgcagcaaa aaaagcttac ggcatcacca atatcagccg
   104161 tgccagagat cttcccttcc cctatccaag tgatttatat attgttgccc caaggatggc
   104221 ctattatatg gaaaagaaca gagaaatcaa ttctatctat aaaaaatatg cagacgaagc
   104281 gaaccatcat gtctattccg tcgatgaaag cttccttgat gtaacaaata gcatggcact
   104341 ctttggtaaa acaaatgcgt acgacatggc taaactgatc cataaagatg tgtttgagca
   104401 gaccggaatc tacacgacta taggaattgg cgataacccg ctacttgcta aactagcctt
   104461 agacaatggc tcaaaagacg gaaagcaaat gtttgctgaa tggcgttatg aagatgtacc
   104521 agaaaagtta tggaaaatct cagatatcac tgacttttgg ggaatcggca aacgaacagc
   104581 aaaacgtctg aaacaaatgg gtatacattc tgtttatgat ttagctcacg cggattatta
   104641 tcacttgaaa agcaagctag gggtcatcgg tactcagctt tatgcccatg cttggggcat
   104701 cgatcgaagt tttttgggac aagaatatac accaaaatca aaaagtatcg gaaatagtca
   104761 aatcctagat agagattata caagacgaga agaaatcgag atcgttatca aagaaatggc
   104821 cgaccaagtc ggcactcgct tgagacgatc tgcagcaaaa acacaagtag tcagcttatg
   104881 ggtaggatac tctttaggct atcttgatcc aaatggcaag accgggtttc accagcaaat
   104941 gaaaatcgaa gcgacaaatg ctagcagaga tctagcagat gctttattga ccattttcca
   105001 ccagcattac cactaccagg atattcggcg tgtaggcgtg aactgttccg acctcgtata
   105061 cgcgacaggt ttacagctta acctttttga agatcctgaa aagcagataa atgaagcgaa
   105121 agttgatttt gttgtcgata cgattagaaa aaaatacggc tttcaatcaa tcatccatgc
   105181 gcatagtctc ttgcaaggag gacgcgccat cgcacgtagt tctctcgttg gcgggcatgc
   105241 aggtggtatg agtggattag aaggtggaaa agatgagacg gacagcaaaa aaattcattg
   105301 actataacga gtatcgagat cggccatttg gattaaaatg ggggactgct tttgcgatgg
   105361 aagaactagt caacggtatc aaaaaaaatg aagaagaagc acgcaaacag cctcttccta
   105421 aacaacaaat gacattgcaa gaaatcgata gtatactgac agaagccttt ttatctcgta
   105481 aagcagtaat cattcagctc aatttaaaag atgaatttgg aagactcttg gatgaggtcg
   105541 aagggcactt cattggtgaa gcctatgaag attactttat gattgaccat cagccgatat
   105601 tgtgggaaga cgtacggtat attgagttaa aggaaacaaa aaaatggttt gtgacatgag
   105661 aggttgtgac agaagtgatc aacttcaaaa aataagaaag gatttttcga aaattgcttc
   105721 ttaaattttt gtggaatttt ggcttatttt tcgaagaagc aacttctgga acaacgtata
   105781 ttagatatca gaggttgtga aaaagtcgct tagttttgag taacaagcca aggcgctgat
   105841 ttcacaacct ctgtctatct atccatctat tcggtaagat tcatgcatga cgtcttcttt
   105901 atttagtatg tagagccctt tttcttctga ccattcaaca taaatcagct gttttacatc
   105961 tgcatagcct tctttcttca agttctccat aagccatgct tcagtcaatt gattggactg
   106021 aagcacttta tgctgaattt gcccttcatc tacaagcagg atcgataaag ctttgtcttc
   106081 atctgatttt ttcaatacac tcatttgacc ggcattctct aggataacat gtttggcatt
   106141 ttcaagaaaa aagcattctt gctgtcttaa gattgctcgc aactgctcca tctcgatatg
   106201 gttatttgtc aactctgtca tgttgatgac gccgtctttg attagaacag atggctcgcc
   106261 tttgacccaa cgattgacct tttcattttt ttggacgata cgttcgatac cataaatcat
   106321 tactgcccaa agcgcaatgg caaataggac atgccctaca tggacattgt catcataggt
   106381 tgattcttct aagatcccac ctaaaacaag cgtatagact aaatcaaacg gtgtgatttc
   106441 tgacatggat ttcttcccaa gcaaacgtac cactaataat aaaccgatca acccgaacac
   106501 cagtttcatt gtaattgaaa aaatcatttt ttctccccct aactatctgt taattaaatt
   106561 atatatgctt tctcacgaac acgcatggaa tatacaagga agaaatgtat ccttttacta
   106621 aaaaaaggac tgtgacactg tagtgacccc caaaagttag acttttttgg agtggagaaa
   106681 tccactcctt ttttttatgc tctaaaatga tatttttaca caaccgttac ggttgtttta
   106741 cgaaattgga cg
//
LOCUS       DAFBQW010000004        93455 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFBQW010000004 DAFBQW010000000
VERSION     DAFBQW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.004"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            85..972
                     /locus_tag="LK889_000398"
     CDS             85..972
                     /locus_tag="LK889_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK5094662.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="LK889_000399"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="LK889_000399"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK5094663.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="LK889_000400"
     CDS             2274..2720
                     /locus_tag="LK889_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5094664.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="LK889_000401"
     CDS             2993..4345
                     /locus_tag="LK889_000401"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBK5094665.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="LK889_000402"
     CDS             4516..4803
                     /locus_tag="LK889_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094666.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="LK889_000403"
     CDS             5029..5472
                     /locus_tag="LK889_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5094667.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="LK889_000404"
     CDS             complement(5478..6662)
                     /locus_tag="LK889_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBK5094668.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="LK889_000405"
     CDS             6930..7628
                     /locus_tag="LK889_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK5094669.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="LK889_000406"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="LK889_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK5094670.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="LK889_000407"
     CDS             complement(8438..8971)
                     /locus_tag="LK889_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094671.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="LK889_000408"
     CDS             complement(9268..11649)
                     /locus_tag="LK889_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK5094672.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="LK889_000409"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="LK889_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBK5094673.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="LK889_000410"
     CDS             12350..12889
                     /locus_tag="LK889_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBK5094674.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="LK889_000411"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="LK889_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBK5094675.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="LK889_000412"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LK889_000412"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="LK889_000413"
     CDS             13996..15162
                     /locus_tag="LK889_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBK5094676.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="LK889_000414"
     CDS             15208..16704
                     /locus_tag="LK889_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBK5094677.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="LK889_000415"
     CDS             16785..17468
                     /locus_tag="LK889_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5094678.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="LK889_000416"
     CDS             18113..19303
                     /locus_tag="LK889_000416"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBK5094679.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="LK889_000417"
     CDS             19405..20358
                     /locus_tag="LK889_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK5094680.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="LK889_000418"
     CDS             20427..21776
                     /locus_tag="LK889_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5094681.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="LK889_000419"
     CDS             complement(21959..22282)
                     /locus_tag="LK889_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094682.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="LK889_000420"
     CDS             22411..23574
                     /locus_tag="LK889_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK5094683.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="LK889_000421"
     CDS             23644..24993
                     /locus_tag="LK889_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5094684.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="LK889_000422"
     CDS             complement(25038..25469)
                     /locus_tag="LK889_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094685.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="LK889_000423"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="LK889_000423"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK5094686.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="LK889_000424"
     CDS             28699..29295
                     /locus_tag="LK889_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5094687.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="LK889_000425"
     CDS             29417..30565
                     /locus_tag="LK889_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK5094688.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="LK889_000426"
     CDS             complement(30603..31016)
                     /locus_tag="LK889_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBK5094689.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="LK889_000427"
     CDS             31095..31526
                     /locus_tag="LK889_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBK5094690.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="LK889_000428"
     CDS             complement(31625..31858)
                     /locus_tag="LK889_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBK5094691.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHQ"
     gene            32041..33711
                     /locus_tag="LK889_000429"
     CDS             32041..33711
                     /locus_tag="LK889_000429"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBK5094692.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="LK889_000430"
     CDS             33858..34244
                     /locus_tag="LK889_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094693.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="LK889_000431"
     CDS             34231..34929
                     /locus_tag="LK889_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5094694.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="LK889_000432"
     CDS             34934..35398
                     /locus_tag="LK889_000432"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK5094695.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="LK889_000433"
     CDS             35395..36306
                     /locus_tag="LK889_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5094696.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="LK889_000434"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="LK889_000434"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBK5094697.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="LK889_000435"
     CDS             37094..38377
                     /locus_tag="LK889_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK5094698.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="LK889_000436"
     CDS             38390..39316
                     /locus_tag="LK889_000436"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBK5094699.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="LK889_000437"
     CDS             39335..40615
                     /locus_tag="LK889_000437"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK5094700.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="LK889_000438"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="LK889_000438"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK5094701.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="LK889_000439"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="LK889_000439"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK5094702.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="LK889_000440"
     CDS             44940..45716
                     /locus_tag="LK889_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBK5094703.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="LK889_000441"
     CDS             45713..46645
                     /locus_tag="LK889_000441"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBK5094704.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="LK889_000442"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="LK889_000442"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBK5094705.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="LK889_000443"
     CDS             47365..47994
                     /locus_tag="LK889_000443"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBK5094706.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="LK889_000444"
     CDS             47982..48296
                     /locus_tag="LK889_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094707.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="LK889_000445"
     CDS             48334..48975
                     /locus_tag="LK889_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5094708.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49018..49710
                     /locus_tag="LK889_000446"
     CDS             49018..49710
                     /locus_tag="LK889_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBK5094709.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="LK889_000447"
     CDS             49707..50609
                     /locus_tag="LK889_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5094710.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="LK889_000448"
     CDS             complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="LK889_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBK5094711.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52663..53286
                     /locus_tag="LK889_000449"
     CDS             52663..53286
                     /locus_tag="LK889_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094712.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53736..54785
                     /locus_tag="LK889_000450"
     CDS             53736..54785
                     /locus_tag="LK889_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5094713.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54782..55663
                     /locus_tag="LK889_000451"
     CDS             54782..55663
                     /locus_tag="LK889_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094714.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55666..56436
                     /locus_tag="LK889_000452"
     CDS             55666..56436
                     /locus_tag="LK889_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5094715.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56715..57569
                     /gene="aroE"
                     /locus_tag="LK889_000453"
     CDS             56715..57569
                     /gene="aroE"
                     /locus_tag="LK889_000453"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK5094716.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57747..58910
                     /locus_tag="LK889_000454"
     CDS             57747..58910
                     /locus_tag="LK889_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5094717.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59027..59437
                     /locus_tag="LK889_000455"
     CDS             59027..59437
                     /locus_tag="LK889_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5094718.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59611..61839
                     /gene="topB"
                     /locus_tag="LK889_000456"
     CDS             59611..61839
                     /gene="topB"
                     /locus_tag="LK889_000456"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5094719.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62010..62294
                     /locus_tag="LK889_000457"
     CDS             62010..62294
                     /locus_tag="LK889_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094720.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            62454..62690
                     /locus_tag="LK889_000458"
     CDS             62454..62690
                     /locus_tag="LK889_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5094721.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62687..63085
                     /locus_tag="LK889_000459"
     CDS             62687..63085
                     /locus_tag="LK889_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK5094722.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63752..64696)
                     /locus_tag="LK889_000460"
     CDS             complement(63752..64696)
                     /locus_tag="LK889_000460"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK5094723.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64992..65936)
                     /locus_tag="LK889_000461"
     CDS             complement(64992..65936)
                     /locus_tag="LK889_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094724.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66157..67371
                     /locus_tag="LK889_000462"
     CDS             66157..67371
                     /locus_tag="LK889_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5094725.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67393..69408
                     /locus_tag="LK889_000463"
     CDS             67393..69408
                     /locus_tag="LK889_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBK5094726.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69491..70864)
                     /locus_tag="LK889_000464"
     CDS             complement(69491..70864)
                     /locus_tag="LK889_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK5094727.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70890..71174)
                     /locus_tag="LK889_000465"
     CDS             complement(70890..71174)
                     /locus_tag="LK889_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5094728.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71176..73191)
                     /locus_tag="LK889_000466"
     CDS             complement(71176..73191)
                     /locus_tag="LK889_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5094729.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73370..73675)
                     /locus_tag="LK889_000467"
     CDS             complement(73370..73675)
                     /locus_tag="LK889_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBK5094730.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73764..74795)
                     /locus_tag="LK889_000468"
     CDS             complement(73764..74795)
                     /locus_tag="LK889_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5094731.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75002..75877
                     /locus_tag="LK889_000469"
     CDS             75002..75877
                     /locus_tag="LK889_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5094732.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76029..76871)
                     /locus_tag="LK889_000470"
     CDS             complement(76029..76871)
                     /locus_tag="LK889_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5094733.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76963..78840)
                     /locus_tag="LK889_000471"
     CDS             complement(76963..78840)
                     /locus_tag="LK889_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5094734.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78837..80258)
                     /locus_tag="LK889_000472"
     CDS             complement(78837..80258)
                     /locus_tag="LK889_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5094735.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80261..83650)
                     /locus_tag="LK889_000473"
     CDS             complement(80261..83650)
                     /locus_tag="LK889_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK5094736.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83907..85298
                     /locus_tag="LK889_000474"
     CDS             83907..85298
                     /locus_tag="LK889_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094737.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85371..86018)
                     /locus_tag="LK889_000475"
     CDS             complement(85371..86018)
                     /locus_tag="LK889_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK5094738.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86026..86712)
                     /locus_tag="LK889_000476"
     CDS             complement(86026..86712)
                     /locus_tag="LK889_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094739.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86718..87791)
                     /locus_tag="LK889_000477"
     CDS             complement(86718..87791)
                     /locus_tag="LK889_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094740.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88013..88687
                     /locus_tag="LK889_000478"
     CDS             88013..88687
                     /locus_tag="LK889_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5094741.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88692..90032
                     /locus_tag="LK889_000479"
     CDS             88692..90032
                     /locus_tag="LK889_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5094742.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90181..91167)
                     /locus_tag="LK889_000480"
     CDS             complement(90181..91167)
                     /locus_tag="LK889_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBK5094743.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91891..92922
                     /locus_tag="LK889_000481"
     CDS             91891..92922
                     /locus_tag="LK889_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5094744.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93031..>93455
                     /locus_tag="LK889_000482"
     CDS             93031..>93455
                     /locus_tag="LK889_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK5094745.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        29896 a        16573 c        18964 g        28022 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct atttcagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattg atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50761 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50821 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50881 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50941 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    51001 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    51061 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    51121 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51181 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51241 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51301 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51361 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51421 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51481 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51541 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51601 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51661 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51721 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51781 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51841 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51901 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51961 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    52021 gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt ctcgattaac
    52081 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    52141 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52201 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52261 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52321 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52381 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52441 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc ttttcataag
    52501 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52561 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52621 ttttaggtat aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa
    52681 gtagttaaat cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat
    52741 ccaagtaagt attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt
    52801 gcaatgtttt tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg
    52861 tccataccaa ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa
    52921 caaagccggg cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg
    52981 cttgatgttt ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat
    53041 ccgatcgttt atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg
    53101 acatttattt tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga
    53161 ataactgatt ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc
    53221 aaacaattag aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct
    53281 aattgagaca ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga
    53341 acaagtgatg tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa
    53401 ttcaaaaatt cgatttgact taaatcaatt cttaaggaaa gggtgacctg taatgaactt
    53461 atcaaaacgt atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt
    53521 attaatggtg agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat
    53581 ctatgctaga catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta
    53641 aagacaatga ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata
    53701 ataaataaaa agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag
    53761 ttgtagtaat tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg
    53821 aaaaagaaag tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat
    53881 acgtgagcca tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag
    53941 aaggattaaa agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga
    54001 gcaagctagc cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa
    54061 tcgcaacacc tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag
    54121 gtgctgtaac ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta
    54181 atgtaacagg tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt
    54241 tgttgccaaa tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat
    54301 accaagtagc acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg
    54361 cggtgccttc cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt
    54421 ttatctatgt tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag
    54481 caaacaaagc aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac
    54541 tagcaacaat cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag
    54601 aacttgcgag tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg
    54661 atacagtgat caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga
    54721 aggaaaaagc caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca
    54781 aatgatcgta tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt
    54841 catgacttat cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg
    54901 tgctgctgtc tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt
    54961 aggcgtaggt gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa
    55021 gataccggtt attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt
    55081 tgtgatgcga acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca
    55141 tcgctttggg ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat
    55201 cttgattgct gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac
    55261 aggagacaac gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt
    55321 aggtttaact ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga
    55381 cggatatgca gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat
    55441 catcggtgaa gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt
    55501 cggcagtatc atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac
    55561 ttatctgaag ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa
    55621 gaaagcactg aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg
    55681 acaatcaaaa atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg
    55741 gatgaaatta accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt
    55801 gctggaaaaa gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg
    55861 atctctttta aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt
    55921 tctcgagttt tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat
    55981 ttgctacttg cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat
    56041 caaaaagaag attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg
    56101 gatactccag cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg
    56161 acgatcacag ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa
    56221 acagctaaag cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt
    56281 ctcatgatca cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc
    56341 caaaaaggga aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc
    56401 gaccttttgc agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag
    56461 tagaaggact tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata
    56521 aacaattaaa catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa
    56581 atagaatatt ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc
    56641 tagacgtttc gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac
    56701 aaaggaggaa taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg
    56761 cttctcacag tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg
    56821 cggtgtattt agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc
    56881 gaacttttga tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt
    56941 atctggatga ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg
    57001 aaaagggtcg tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag
    57061 aagctgggat ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa
    57121 aggcagtcac tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa
    57181 acaaccaaac attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt
    57241 gtaagataaa agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa
    57301 gctgtcttct agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc
    57361 tcttagatat aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta
    57421 gagaaacacg tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg
    57481 gcatgttgat ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga
    57541 tccatgtgat tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct
    57601 cgcataaacg gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt
    57661 tttattaaat tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta
    57721 tcagtaagaa acgaggagga accacaatga gtcggatgga tcgctataag gacatccata
    57781 agaaggcgaa acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac
    57841 gaacgcgtat ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat
    57901 ttgaacattt gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac
    57961 aacaaaaaaa taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa
    58021 gaaagcgatc gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag
    58081 gtatctcatt ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga
    58141 cttcagaaac ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg
    58201 gaagtgatac acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg
    58261 atggtccttc ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc
    58321 caggggtagg acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc
    58381 gtcaaacttt agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca
    58441 aatcgtttga aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa
    58501 aagatctgaa tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg
    58561 ttcttttgca gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac
    58621 gccaacagca agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc
    58681 gtgcacctta tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt
    58741 tcttgacgaa aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg
    58801 tgccagtaga cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa
    58861 ttgataatca aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa
    58921 gatcaagaag aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa
    58981 aaacgctttc ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct
    59041 gaacttcaaa aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga
    59101 agcatcaggt atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga
    59161 agaagcaaaa aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt
    59221 tgcagatacg ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt
    59281 catccagaca agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg
    59341 taagattgag atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga
    59401 tcgatttggc atcaactggc agattaacca agagtgacag ctaaagatga atattttctg
    59461 gaaacatcaa gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg
    59521 actatacggt atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc
    59581 cgcaaaaaat taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct
    59641 tcacaagcaa aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat
    59701 gaaatcagcg atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg
    59761 gtagatatgg ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat
    59821 ttgcctattt tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt
    59881 tcgatcgtaa aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat
    59941 cgagaaggtg aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa
    60001 aagaactatt tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt
    60061 aagaatctcc aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag
    60121 aacgctgatt ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga
    60181 aaagggattg atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt
    60241 tatcagctgc aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt
    60301 ttaattacga caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa
    60361 acccaagaag agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac
    60421 ggacggattc ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg
    60481 ctatctagtt tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt
    60541 gatgctgctc aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt
    60601 tacattacag aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt
    60661 ttaggaatag aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg
    60721 aaaaaagttc aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc
    60781 tttgcgaagc tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg
    60841 gctatgtttg ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa
    60901 ttaaagctaa aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc
    60961 atcaagtcaa aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca
    61021 gaagtagaac tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga
    61081 acgttgatca cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa
    61141 attctcaaag aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat
    61201 cttaagcaga agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga
    61261 atcactcttt gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg
    61321 cgctgggaag ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta
    61381 gagaatatca aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag
    61441 gtagactttt cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac
    61501 gatcaattag gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc
    61561 gctacatgct tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag
    61621 ctgactaaaa cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa
    61681 ggttttactg gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca
    61741 atcggttttt cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa
    61801 gaaacatcaa gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg
    61861 aatatttatt ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat
    61921 caagcaaaaa cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga
    61981 aaaaagtgca ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc
    62041 aagcatttct aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa
    62101 cagcatattc accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt
    62161 atttttttca taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg
    62221 tggtggtagc ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc
    62281 aatattttgg ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt
    62341 tgtcattttt tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac
    62401 ttggatataa tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa
    62461 aaatgaaaaa gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag
    62521 aagtaaaaga attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg
    62581 atggttctgt acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt
    62641 cgatcatgaa tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc
    62701 gactaaagaa attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat
    62761 gatcggtgta aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt
    62821 attcaatcaa gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt
    62881 agctaaaaaa catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt
    62941 gtttcttcaa ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat
    63001 cataatgatt accactagtt cattggattt cgattcacta aagtccaaga taaccagtta
    63061 tttacgacaa accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc
    63121 atgtcattaa ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata
    63181 aaaatatgga aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt
    63241 gaatagtagg aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac
    63301 ataatatata ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt
    63361 atcaaaaatc gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc
    63421 agtttttctt actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt
    63481 taatcaaata tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt
    63541 taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt
    63601 catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt
    63661 tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt
    63721 tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta
    63781 aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa
    63841 cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt
    63901 cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag
    63961 cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa
    64021 tgatctcata agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa
    64081 tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc
    64141 atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt
    64201 caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag
    64261 taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat
    64321 ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta
    64381 cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt
    64441 tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac
    64501 ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt
    64561 ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt
    64621 tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac
    64681 gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa
    64741 agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt
    64801 tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat
    64861 atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc
    64921 aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa
    64981 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
    65041 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
    65101 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
    65161 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
    65221 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
    65281 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
    65341 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta
    65401 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
    65461 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt
    65521 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
    65581 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
    65641 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
    65701 ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
    65761 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
    65821 aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
    65881 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
    65941 aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct
    66001 tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt
    66061 ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact
    66121 atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg
    66181 aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca
    66241 attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg
    66301 actgctccct cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa
    66361 atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc
    66421 cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc
    66481 tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa
    66541 cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca
    66601 tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc
    66661 agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt
    66721 caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt
    66781 tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc
    66841 acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc
    66901 atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat
    66961 cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact
    67021 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
    67081 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
    67141 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
    67201 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
    67261 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
    67321 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
    67381 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
    67441 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
    67501 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
    67561 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
    67621 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
    67681 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
    67741 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
    67801 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
    67861 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
    67921 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
    67981 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
    68041 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
    68101 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
    68161 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta
    68221 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
    68281 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
    68341 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
    68401 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
    68461 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
    68521 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
    68581 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
    68641 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
    68701 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
    68761 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
    68821 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
    68881 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
    68941 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
    69001 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
    69061 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
    69121 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
    69181 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
    69241 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
    69301 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
    69361 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
    69421 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
    69481 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
    69541 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
    69601 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
    69661 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
    69721 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
    69781 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
    69841 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
    69901 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
    69961 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
    70021 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
    70081 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
    70141 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
    70201 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
    70261 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
    70321 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
    70381 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
    70441 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
    70501 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
    70561 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
    70621 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
    70681 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
    70741 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
    70801 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
    70861 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
    70921 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
    70981 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
    71041 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
    71101 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
    71161 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
    71221 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
    71281 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
    71341 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
    71401 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
    71461 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
    71521 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
    71581 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
    71641 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
    71701 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
    71761 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
    71821 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
    71881 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
    71941 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
    72001 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
    72061 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
    72121 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
    72181 gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga
    72241 ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg
    72301 aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca
    72361 ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca
    72421 aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac
    72481 ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc
    72541 catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat
    72601 tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac
    72661 tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc
    72721 aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga
    72781 ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa
    72841 ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc
    72901 ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc
    72961 tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc
    73021 ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa
    73081 aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc
    73141 tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt
    73201 cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac
    73261 ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa
    73321 aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat
    73381 catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt
    73441 cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg
    73501 aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt
    73561 cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt
    73621 taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg
    73681 ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa
    73741 caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt
    73801 ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg
    73861 ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa
    73921 attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt
    73981 tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct
    74041 tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt
    74101 ggacgttctt tgatgcggaa ataactagat tttatcgaaa ttttcatatt tccatagaaa
    74161 aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag
    74221 ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt
    74281 ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg
    74341 aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact
    74401 ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga
    74461 cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt
    74521 ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat
    74581 tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc
    74641 tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt
    74701 tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta
    74761 ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt
    74821 tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt
    74881 tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa
    74941 atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat
    75001 catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat
    75061 tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc
    75121 aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag
    75181 cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt
    75241 caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc
    75301 aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca
    75361 tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc
    75421 aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa
    75481 gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag
    75541 cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat
    75601 gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga
    75661 aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa
    75721 agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt
    75781 gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat
    75841 gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata
    75901 agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc
    75961 tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg
    76021 aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg
    76081 aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat
    76141 actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca
    76201 agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc
    76261 cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact
    76321 tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc
    76381 ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt
    76441 gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc
    76501 agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa
    76561 ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta
    76621 gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc
    76681 atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa
    76741 tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct
    76801 agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc
    76861 tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat
    76921 ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt
    76981 gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac
    77041 atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc
    77101 aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg
    77161 gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata
    77221 ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc
    77281 tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt
    77341 gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac
    77401 gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt
    77461 ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc
    77521 tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga
    77581 aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata
    77641 atttactggg tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga
    77701 atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt
    77761 gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg
    77821 aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt
    77881 agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa
    77941 aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa
    78001 attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc
    78061 ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt
    78121 ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct
    78181 tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc
    78241 agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag
    78301 atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat
    78361 ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc
    78421 ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac
    78481 atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac
    78541 tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt
    78601 gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat
    78661 ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag
    78721 gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag
    78781 taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat
    78841 tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata
    78901 ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta
    78961 ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa
    79021 gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc
    79081 tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg
    79141 gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc
    79201 tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct
    79261 ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt
    79321 gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt
    79381 gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc
    79441 aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct
    79501 tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct
    79561 tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc
    79621 gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat
    79681 accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata
    79741 gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc
    79801 ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt
    79861 tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta
    79921 gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt
    79981 gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa
    80041 acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg
    80101 tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc
    80161 tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac
    80221 aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt
    80281 ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact agatagattc
    80341 ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta
    80401 aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca
    80461 cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc
    80521 cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa
    80581 aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc
    80641 tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa
    80701 acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt
    80761 ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag
    80821 tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat
    80881 caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat
    80941 ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc
    81001 cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca
    81061 actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag
    81121 aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg
    81181 tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg
    81241 ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag
    81301 cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg
    81361 tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta
    81421 caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta
    81481 gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac
    81541 gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc
    81601 aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat
    81661 cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag
    81721 ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag
    81781 ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga
    81841 ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct
    81901 tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac
    81961 tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt
    82021 gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat
    82081 ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt
    82141 gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt
    82201 ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg
    82261 ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga
    82321 ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt
    82381 aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac
    82441 catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg
    82501 catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt
    82561 ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat
    82621 atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg
    82681 ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt
    82741 tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta
    82801 catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat
    82861 actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg
    82921 tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt
    82981 tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat
    83041 aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca
    83101 cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg
    83161 atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt
    83221 ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt
    83281 ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga
    83341 tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt
    83401 ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg
    83461 tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt
    83521 cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg
    83581 gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg
    83641 tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt
    83701 attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa
    83761 gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt
    83821 ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca
    83881 ggttgttgga atgaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg
    83941 acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg
    84001 taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac
    84061 aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg
    84121 aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta
    84181 aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt
    84241 tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt
    84301 ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt
    84361 taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa
    84421 ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat
    84481 ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg
    84541 aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa
    84601 tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt
    84661 ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga
    84721 tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag
    84781 aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata
    84841 cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac
    84901 gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc
    84961 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
    85021 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
    85081 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
    85141 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
    85201 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
    85261 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
    85321 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
    85381 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
    85441 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
    85501 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
    85561 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
    85621 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
    85681 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
    85741 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
    85801 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
    85861 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
    85921 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
    85981 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
    86041 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
    86101 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
    86161 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
    86221 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
    86281 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
    86341 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
    86401 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
    86461 gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat
    86521 ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga
    86581 accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt
    86641 caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa
    86701 tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta
    86761 cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac
    86821 cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa
    86881 gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa
    86941 gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc
    87001 ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa
    87061 aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg
    87121 aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct
    87181 gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt
    87241 ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa
    87301 taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc
    87361 gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt
    87421 gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg
    87481 aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt
    87541 gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat
    87601 acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt
    87661 ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc
    87721 aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata
    87781 gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt
    87841 gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa
    87901 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
    87961 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
    88021 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
    88081 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
    88141 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
    88201 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
    88261 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
    88321 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
    88381 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
    88441 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
    88501 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
    88561 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
    88621 aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag
    88681 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
    88741 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
    88801 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
    88861 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
    88921 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
    88981 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
    89041 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
    89101 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
    89161 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
    89221 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
    89281 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
    89341 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
    89401 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
    89461 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
    89521 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
    89581 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg
    89641 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa
    89701 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
    89761 agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg
    89821 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
    89881 aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
    89941 tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta
    90001 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
    90061 aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc
    90121 ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc
    90181 ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc
    90241 ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc
    90301 aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat
    90361 agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg
    90421 tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt
    90481 gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa
    90541 tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc
    90601 agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg
    90661 tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg
    90721 atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc
    90781 ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac
    90841 aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc
    90901 cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg
    90961 gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc
    91021 aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata
    91081 cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc
    91141 gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg
    91201 gtatattgtt acttataaat ttattctata ggagacgatt gttttctaca aatatttaga
    91261 ttggaatttg attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca
    91321 tattctaata aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt
    91381 ccactcgttg tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa
    91441 aaggattaat tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg
    91501 aagtaccaac tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac
    91561 cattattgaa taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa
    91621 ataaaagaaa gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt
    91681 aaccacatag ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat
    91741 aaaataacta aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat
    91801 ttgcttccta agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata
    91861 gaaaatgaac actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa
    91921 gaaaagttaa agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa
    91981 aatcgttctt ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt
    92041 actgattatt tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta
    92101 gaaaaacaag attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa
    92161 actacagcga accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg
    92221 aagcaatatt cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa
    92281 gagatttctc ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc
    92341 gatgcagaac ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca
    92401 agcagagaag ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt
    92461 cttctgtatt gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct
    92521 ttgaccaagc agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata
    92581 ccaaaagaag cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac
    92641 gaaaattctt ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc
    92701 caaattttga gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca
    92761 tatcgttatt atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt
    92821 aatcgatacc tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct
    92881 tatcacgaat tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt
    92941 ttttcacaat aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta
    93001 tattacttaa caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg
    93061 aacttattta cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt
    93121 gatgagccac tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc
    93181 gaaagtgtgt tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa
    93241 gttattattt ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac
    93301 cttggtgcag tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt
    93361 atcgatcgtt tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga
    93421 gaaatttcaa atggaacaaa atcaaaacag attcc
//
LOCUS       DAFBQW010000005        92611 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFBQW010000005 DAFBQW010000000
VERSION     DAFBQW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.005"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            160..840
                     /locus_tag="LK889_000483"
     CDS             160..840
                     /locus_tag="LK889_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK5094746.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="LK889_000484"
     CDS             930..1964
                     /locus_tag="LK889_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK5094747.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="LK889_000485"
     CDS             1978..2313
                     /locus_tag="LK889_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094748.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="LK889_000486"
     CDS             2297..4777
                     /locus_tag="LK889_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094749.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="LK889_000487"
     CDS             4833..6392
                     /locus_tag="LK889_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094750.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="LK889_000488"
     CDS             6389..7753
                     /locus_tag="LK889_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5094751.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="LK889_000489"
     CDS             7924..8994
                     /locus_tag="LK889_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094752.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="LK889_000490"
     CDS             9009..9677
                     /locus_tag="LK889_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094753.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="LK889_000491"
     CDS             9697..10275
                     /locus_tag="LK889_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5094754.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="LK889_000492"
     CDS             10676..11587
                     /locus_tag="LK889_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5094755.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="LK889_000493"
     CDS             complement(11965..12882)
                     /locus_tag="LK889_000493"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBK5094756.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="LK889_000494"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="LK889_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK5094757.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="LK889_000495"
     CDS             13579..14127
                     /locus_tag="LK889_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBK5094758.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="LK889_000496"
     CDS             14242..16602
                     /locus_tag="LK889_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK5094759.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="LK889_000497"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="LK889_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBK5094760.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="LK889_000498"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="LK889_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK5094761.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="LK889_000499"
     CDS             19108..20007
                     /locus_tag="LK889_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094762.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="LK889_000500"
     CDS             20000..21262
                     /locus_tag="LK889_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094763.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="LK889_000501"
     CDS             21423..23321
                     /locus_tag="LK889_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBK5094764.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="LK889_000502"
     CDS             complement(23651..24370)
                     /locus_tag="LK889_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBK5094765.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24706..25038
                     /locus_tag="LK889_000503"
     CDS             24706..25038
                     /locus_tag="LK889_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094766.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25025..27028
                     /locus_tag="LK889_000504"
     CDS             25025..27028
                     /locus_tag="LK889_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBK5094767.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27047..27517
                     /locus_tag="LK889_000505"
     CDS             27047..27517
                     /locus_tag="LK889_000505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBK5094768.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27538..28122
                     /locus_tag="LK889_000506"
     CDS             27538..28122
                     /locus_tag="LK889_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBK5094769.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28137..29123
                     /locus_tag="LK889_000507"
     CDS             28137..29123
                     /locus_tag="LK889_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBK5094770.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29113..29424
                     /locus_tag="LK889_000508"
     CDS             29113..29424
                     /locus_tag="LK889_000508"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBK5094771.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29446..31227
                     /locus_tag="LK889_000509"
     CDS             29446..31227
                     /locus_tag="LK889_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBK5094772.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31220..32596
                     /locus_tag="LK889_000510"
     CDS             31220..32596
                     /locus_tag="LK889_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBK5094773.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32603..33238
                     /locus_tag="LK889_000511"
     CDS             32603..33238
                     /locus_tag="LK889_000511"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBK5094774.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33268..34623
                     /locus_tag="LK889_000512"
     CDS             33268..34623
                     /locus_tag="LK889_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBK5094775.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34815..36209)
                     /locus_tag="LK889_000513"
     CDS             complement(34815..36209)
                     /locus_tag="LK889_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK5094776.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36301..37272
                     /locus_tag="LK889_000514"
     CDS             36301..37272
                     /locus_tag="LK889_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBK5094777.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37269..38051
                     /locus_tag="LK889_000515"
     CDS             37269..38051
                     /locus_tag="LK889_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBK5094778.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38220..38591)
                     /locus_tag="LK889_000516"
     CDS             complement(38220..38591)
                     /locus_tag="LK889_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5094779.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38725..39168
                     /locus_tag="LK889_000517"
     CDS             38725..39168
                     /locus_tag="LK889_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBK5094780.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39211..40020)
                     /gene="yidA"
                     /locus_tag="LK889_000518"
     CDS             complement(39211..40020)
                     /gene="yidA"
                     /locus_tag="LK889_000518"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK5094781.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40039..41406)
                     /locus_tag="LK889_000519"
     CDS             complement(40039..41406)
                     /locus_tag="LK889_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5094782.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEYIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41390..42220)
                     /locus_tag="LK889_000520"
     CDS             complement(41390..42220)
                     /locus_tag="LK889_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBK5094783.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42383..42820
                     /locus_tag="LK889_000521"
     CDS             42383..42820
                     /locus_tag="LK889_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBK5094784.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42896..43516
                     /locus_tag="LK889_000522"
     CDS             42896..43516
                     /locus_tag="LK889_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBK5094785.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43705..44211
                     /locus_tag="LK889_000523"
     CDS             43705..44211
                     /locus_tag="LK889_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBK5094786.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44228..45127
                     /locus_tag="LK889_000524"
     CDS             44228..45127
                     /locus_tag="LK889_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBK5094787.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45214..46098
                     /locus_tag="LK889_000525"
     CDS             45214..46098
                     /locus_tag="LK889_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK5094788.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46290..46361)
                     /locus_tag="LK889_000526"
     tRNA            complement(46290..46361)
                     /locus_tag="LK889_000526"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46326..46328),aa:Arg,seq:ccg)
     gene            46421..47755
                     /gene="rpoN"
                     /locus_tag="LK889_000527"
     CDS             46421..47755
                     /gene="rpoN"
                     /locus_tag="LK889_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK5094789.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47904..48995
                     /locus_tag="LK889_000528"
     CDS             47904..48995
                     /locus_tag="LK889_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBK5094790.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49036..50037
                     /gene="gap"
                     /locus_tag="LK889_000529"
     CDS             49036..50037
                     /gene="gap"
                     /locus_tag="LK889_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK5094791.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50147..51337
                     /locus_tag="LK889_000530"
     CDS             50147..51337
                     /locus_tag="LK889_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK5094792.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51441..52196
                     /locus_tag="LK889_000531"
     CDS             51441..52196
                     /locus_tag="LK889_000531"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK5094793.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52312..53610
                     /gene="eno"
                     /locus_tag="LK889_000532"
     CDS             52312..53610
                     /gene="eno"
                     /locus_tag="LK889_000532"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK5094794.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            54010..55149
                     /locus_tag="LK889_000533"
     CDS             54010..55149
                     /locus_tag="LK889_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBK5094795.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEELTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(55216..55791)
                     /locus_tag="LK889_000534"
     CDS             complement(55216..55791)
                     /locus_tag="LK889_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5094796.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(55796..56770)
                     /locus_tag="LK889_000535"
     CDS             complement(55796..56770)
                     /locus_tag="LK889_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBK5094797.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(56794..56838)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            57053..58021
                     /locus_tag="LK889_000536"
     CDS             57053..58021
                     /locus_tag="LK889_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5094798.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            58134..58412
                     /locus_tag="LK889_000537"
     CDS             58134..58412
                     /locus_tag="LK889_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK5094799.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCTYAKKEFEKKPEYQDVLRQS"
     gene            58530..58766
                     /gene="secG"
                     /locus_tag="LK889_000538"
     CDS             58530..58766
                     /gene="secG"
                     /locus_tag="LK889_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK5094800.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            58869..59624
                     /locus_tag="LK889_000539"
     CDS             58869..59624
                     /locus_tag="LK889_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5094801.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            59627..61984
                     /gene="rnr"
                     /locus_tag="LK889_000540"
     CDS             59627..61984
                     /gene="rnr"
                     /locus_tag="LK889_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBK5094802.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            62006..62470
                     /gene="smpB"
                     /locus_tag="LK889_000541"
     CDS             62006..62470
                     /gene="smpB"
                     /locus_tag="LK889_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBK5094803.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            62772..63491
                     /gene="atpB"
                     /locus_tag="LK889_000542"
     CDS             62772..63491
                     /gene="atpB"
                     /locus_tag="LK889_000542"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK5094804.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            63544..63759
                     /gene="atpE"
                     /locus_tag="LK889_000543"
     CDS             63544..63759
                     /gene="atpE"
                     /locus_tag="LK889_000543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBK5094805.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            63850..64374
                     /gene="atpF"
                     /locus_tag="LK889_000544"
     CDS             63850..64374
                     /gene="atpF"
                     /locus_tag="LK889_000544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK5094806.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            64361..64903
                     /locus_tag="LK889_000545"
     CDS             64361..64903
                     /locus_tag="LK889_000545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK5094807.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            64926..66482
                     /locus_tag="LK889_000546"
     CDS             64926..66482
                     /locus_tag="LK889_000546"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK5094808.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            66496..67398
                     /locus_tag="LK889_000547"
     CDS             66496..67398
                     /locus_tag="LK889_000547"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK5094809.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            67421..68827
                     /gene="atpD"
                     /locus_tag="LK889_000548"
     CDS             67421..68827
                     /gene="atpD"
                     /locus_tag="LK889_000548"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK5094810.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            68841..69263
                     /locus_tag="LK889_000549"
     CDS             68841..69263
                     /locus_tag="LK889_000549"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK5094811.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            69732..69962
                     /locus_tag="LK889_000550"
     CDS             69732..69962
                     /locus_tag="LK889_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBK5094812.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            70059..71360
                     /gene="murA"
                     /locus_tag="LK889_000551"
     CDS             70059..71360
                     /gene="murA"
                     /locus_tag="LK889_000551"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK5094813.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            71373..71549
                     /locus_tag="LK889_000552"
     CDS             71373..71549
                     /locus_tag="LK889_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK5094814.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            71684..71980
                     /gene="yidD"
                     /locus_tag="LK889_000553"
     CDS             71684..71980
                     /gene="yidD"
                     /locus_tag="LK889_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK5094815.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            72164..72631
                     /locus_tag="LK889_000554"
     CDS             72164..72631
                     /locus_tag="LK889_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094816.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            72716..73600
                     /locus_tag="LK889_000555"
     CDS             72716..73600
                     /locus_tag="LK889_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5094817.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            73745..73951
                     /locus_tag="LK889_000556"
     CDS             73745..73951
                     /locus_tag="LK889_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBK5094818.1"
                     /translation="MRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGPKEYEIFFGE
                     EDFGKEKSLENAIETVIRHWNLHE"
     gene            74039..74119
                     /locus_tag="LK889_000557"
     tRNA            74039..74119
                     /locus_tag="LK889_000557"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74073..74075,aa:Tyr,seq:gta)
     gene            74126..74197
                     /locus_tag="LK889_000558"
     tRNA            74126..74197
                     /locus_tag="LK889_000558"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74158..74160,aa:Gln,seq:ttg)
     gene            74322..74492
                     /locus_tag="LK889_000559"
     CDS             74322..74492
                     /locus_tag="LK889_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094819.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            74712..75419
                     /locus_tag="LK889_000560"
     CDS             74712..75419
                     /locus_tag="LK889_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5094820.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(75479..76123)
                     /locus_tag="LK889_000561"
     CDS             complement(75479..76123)
                     /locus_tag="LK889_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBK5094821.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            76250..76537
                     /locus_tag="LK889_000562"
     CDS             76250..76537
                     /locus_tag="LK889_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094822.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            76645..76965
                     /locus_tag="LK889_000563"
     CDS             76645..76965
                     /locus_tag="LK889_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5094823.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            77046..78359
                     /locus_tag="LK889_000564"
     CDS             77046..78359
                     /locus_tag="LK889_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK5094824.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            78356..79075
                     /locus_tag="LK889_000565"
     CDS             78356..79075
                     /locus_tag="LK889_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBK5094825.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            79203..79754
                     /gene="raiA"
                     /locus_tag="LK889_000566"
     CDS             79203..79754
                     /gene="raiA"
                     /locus_tag="LK889_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK5094826.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            79976..82510
                     /gene="secA"
                     /locus_tag="LK889_000567"
     CDS             79976..82510
                     /gene="secA"
                     /locus_tag="LK889_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK5094827.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            82636..83737
                     /gene="prfB"
                     /locus_tag="LK889_000568"
     CDS             join(82636..82707,82709..83737)
                     /gene="prfB"
                     /locus_tag="LK889_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK5094828.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            83842..84528
                     /gene="ftsE"
                     /locus_tag="LK889_000569"
     CDS             83842..84528
                     /gene="ftsE"
                     /locus_tag="LK889_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK5094829.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            84512..85405
                     /locus_tag="LK889_000570"
     CDS             84512..85405
                     /locus_tag="LK889_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094830.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            85557..87005
                     /gene="cls"
                     /locus_tag="LK889_000571"
     CDS             85557..87005
                     /gene="cls"
                     /locus_tag="LK889_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK5094831.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            87342..88199
                     /locus_tag="LK889_000572"
     CDS             87342..88199
                     /locus_tag="LK889_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK5094832.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            88314..89234
                     /gene="pstC"
                     /locus_tag="LK889_000573"
     CDS             88314..89234
                     /gene="pstC"
                     /locus_tag="LK889_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBK5094833.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            89234..90118
                     /gene="pstA"
                     /locus_tag="LK889_000574"
     CDS             89234..90118
                     /gene="pstA"
                     /locus_tag="LK889_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK5094834.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            90133..90939
                     /gene="pstB"
                     /locus_tag="LK889_000575"
     CDS             90133..90939
                     /gene="pstB"
                     /locus_tag="LK889_000575"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK5094835.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            90955..91713
                     /locus_tag="LK889_000576"
     CDS             90955..91713
                     /locus_tag="LK889_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK5094836.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            91726..92403
                     /gene="phoU"
                     /locus_tag="LK889_000577"
     CDS             91726..92403
                     /gene="phoU"
                     /locus_tag="LK889_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK5094837.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT        30685 a        16129 c        19804 g        25993 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaatta
    24421 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    24481 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    24541 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    24601 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    24661 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    24721 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    24781 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    24841 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    24901 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    24961 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    25021 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    25081 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    25141 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    25201 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    25261 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    25321 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    25381 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    25441 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    25501 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    25561 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    25621 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    25681 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    25741 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    25801 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    25861 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    25921 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    25981 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    26041 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    26101 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    26161 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    26221 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    26281 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    26341 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    26401 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    26461 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    26521 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    26581 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    26641 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    26701 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    26761 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    26821 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    26881 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    26941 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    27001 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    27061 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    27121 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    27181 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca
    27241 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    27301 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    27361 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    27421 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    27481 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    27541 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    27601 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    27661 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    27721 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    27781 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    27841 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    27901 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    27961 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    28021 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    28081 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    28141 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    28201 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    28261 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    28321 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    28381 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    28441 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    28501 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    28561 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    28621 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    28681 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    28741 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    28801 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    28861 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    28921 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    28981 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    29041 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    29101 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    29161 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    29221 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    29281 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    29341 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    29401 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    29461 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    29521 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    29581 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    29641 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    29701 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    29761 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    29821 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    29881 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    29941 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    30001 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    30061 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    30121 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    30181 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    30241 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    30301 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    30361 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    30421 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    30481 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    30541 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    30601 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    30661 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    30721 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    30781 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    30841 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    30901 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    30961 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    31021 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    31081 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    31141 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    31201 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    31261 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    31321 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    31381 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    31441 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    31501 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    31561 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    31621 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    31681 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    31741 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    31801 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    31861 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    31921 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    31981 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    32041 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    32101 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    32161 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    32221 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    32281 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    32341 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    32401 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    32461 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    32521 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    32581 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    32641 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    32701 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    32761 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    32821 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    32881 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    32941 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    33001 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    33061 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    33121 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    33181 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    33241 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    33301 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    33361 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    33421 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    33481 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    33541 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    33601 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    33661 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    33721 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    33781 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    33841 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    33901 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    33961 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    34021 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    34081 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    34141 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    34201 ggattgaaaa caactacgtt aggtgtgctg ctgatccaga tgcgttcgat gttgaaagga
    34261 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    34321 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    34381 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    34441 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    34501 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    34561 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    34621 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    34681 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    34741 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    34801 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    34861 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    34921 gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    34981 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    35041 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    35101 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    35161 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    35221 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    35281 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    35341 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    35401 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    35461 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    35521 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    35581 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    35641 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    35701 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    35761 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    35821 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    35881 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    35941 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    36001 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    36061 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    36121 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    36181 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    36241 aattttacca ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct
    36301 atgaaaatta ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa
    36361 gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg
    36421 gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg
    36481 ggggagaaca gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat
    36541 catttacctt ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa
    36601 gcattgataa acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca
    36661 acagattact tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa
    36721 gcattgtcgc atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac
    36781 gatattttag aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa
    36841 ataacgttta taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt
    36901 acgttgacct ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat
    36961 atcaatgctg tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa
    37021 acttctcaaa tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt
    37081 atagccatgg cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta
    37141 gtttctgaga aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt
    37201 ggtctagcta ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg
    37261 gctttaaaat gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag
    37321 gaaatccagc agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa
    37381 ttgccattga aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg
    37441 agcttagatg gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa
    37501 cagcatttgt tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc
    37561 aaagcggtaa tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta
    37621 ttatgccgaa aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta
    37681 aagaagccta cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta
    37741 aattgcagcc agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga
    37801 cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca
    37861 atgaagatcc agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa
    37921 tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg
    37981 aattaaaaga agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct
    38041 cgtttatttg atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat
    38101 agctacaatg gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc
    38161 tcattttaat tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac
    38221 tatcctcgga tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc
    38281 gcagtgccag gtaatccttt taagtcagta atctcaagac cagcagcttt atgttgctcg
    38341 tgtaattttt ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca
    38401 tacgcaccat gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc
    38461 aaataaacaa gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca
    38521 aatgcctttt gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga
    38581 gccattttca tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa
    38641 ggaaatagtt gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata
    38701 aaactgttaa ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt
    38761 gctgattatc aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca
    38821 gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag
    38881 acgaaagaag aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt
    38941 tcttcttatc tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat
    39001 tataatccag gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa
    39061 aagaccaata aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt
    39121 agccaccgat gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt
    39181 agatcagctt tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc
    39241 tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac
    39301 ggcatttccc attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt
    39361 atctccgata gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac
    39421 tgccgcacct ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac
    39481 cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc
    39541 tggttcgtcg atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt
    39601 tcgatatttt agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc
    39661 tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg
    39721 tgccattact tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt
    39781 acctgatttc gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg
    39841 aaaaaggttc aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa
    39901 aacgatcgaa acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac
    39961 ttctcttttc tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat
    40021 aatttcctcc aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc
    40081 aaattcttga taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat
    40141 ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag
    40201 ctgactaacc tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt
    40261 gcgatgtctg ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac
    40321 agcagtgtaa taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac
    40381 taattttgaa tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa
    40441 acgtttagct aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt
    40501 gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa
    40561 tggcagaagt aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg
    40621 tgcgcgatga agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta
    40681 cacttgcaca tacatttgat atcggctaac gatataatct tccactgcat gcatgccact
    40741 catggaaaaa gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc
    40801 aaatgttcca tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc
    40861 tgcatcgatc tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat
    40921 cacactagct accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc
    40981 tgtttctgga gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatata
    41041 ttcaaatgta tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt
    41101 caccagccgt tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg
    41161 agaaaagata tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg
    41221 gctatgttct gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag
    41281 acgttggact tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata
    41341 gttatggact ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt
    41401 tgtcataatg tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc
    41461 attgctccag ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag
    41521 aatacagctt ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt
    41581 ggtcaagcag ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg
    41641 taccaaagtc ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccacgttttt
    41701 cctgattacc gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct
    41761 gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag
    41821 aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga
    41881 gcatttgctc gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca
    41941 aagaaatatt caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca
    42001 ctggagcgta acctgcatct tccagtgttt ggatcccgtc acttagaaaa ggagttcgaa
    42061 catccttcat acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct
    42121 ggtgttcttt agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt
    42181 tttgataaat gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata
    42241 aattcatatt ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt
    42301 ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat
    42361 gagaagtaaa ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt
    42421 gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca
    42481 aatggtgaca attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg
    42541 cgatgagatt cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc
    42601 aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg
    42661 aacaccatac ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa
    42721 agaccgtccc acagcaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa
    42781 aatcggcgaa tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat
    42841 tttttgtgtc tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga
    42901 aattagtgta tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc
    42961 gatcttagaa caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa
    43021 ctatcttggg aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt
    43081 gaatattgac ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta
    43141 tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac
    43201 tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat
    43261 cgattacaat gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct
    43321 gtttgatgac gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga
    43381 aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga
    43441 tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga
    43501 ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta
    43561 tcagaataag gaggtgtgac acgatttttg tcacaccttc ttttttgtat ataaaaatcc
    43621 caagtaaata cataaaaaat aaaaaaattg aaacttttag cgcaccttgt acgtcatatt
    43681 aagtgaggca gaaaggagtg gagaatgatt tttactggat ataagaaaaa acggcagatc
    43741 gaaaaatacc gtcacctgct gaatggactc atcgaaactg attatcaaaa aatgttccgt
    43801 atcacgatga attacgtaca tgataaagaa gaggccttag atgtgatgca agatagtttt
    43861 cataaagccc tgaactattt tgaaaaagaa aaagagatcg agcattttag tgcatggttt
    43921 tatcggatat tgatccgcac ggcattagat agttggcgca gacaaaaaag gacgcctgtg
    43981 gacttatttg aaattcaaga tatcccccaa cagttacctt tagaaacttc cgttgcagag
    44041 ctccatatga ttttagataa aatagacagt ccggaaaaag agattctgat cctctacttt
    44101 tttgaaggat ttcgtttgaa agaaattgcg actatcttgg atatgaatga aaacacagtt
    44161 aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg aaggagagaa
    44221 gagtgccatg tattcccaaa aagaaaattt aaaaaaacta aagaaaactt ataaagaaca
    44281 agcaattccc aaagggctta aagaggagat ccgccagcgt tttattatcg aagagcaatc
    44341 atttataaaa gcaaagcgga gaaagagacg tctccaaggg ttcggagtat ctttggctat
    44401 cgtaatgatt acaacaagta gccttctttt taatagtcaa gttcgaagct ttgcagaaga
    44461 tttacccatc ttaggatcag tcattgaatt gatcttaggc gaacgcttta ctgatcgatc
    44521 tgaaaaaatc gatattcagg taccgaagat caatacacaa aatgagaaag aaaataaaac
    44581 aattcatgga ttaaatcaaa aatactttcg cgaaggacaa gcagattttg aaaaagctga
    44641 aaaagaatat ggaaattttg aaacggatca ttaccaagtt ttgggagatt atcagaaaat
    44701 cgtagacgat aaccggtttc tcgtgattga aaggcagatc acccaaacag cagcagatag
    44761 ccatgttgaa aaaagatacg acacaatcga caagaaaaac agtgtccagc tctcacttcc
    44821 acttttattc aaagatgaca cctatctatc tgttttgaca aaagaagtca aacggcagat
    44881 ggccgaacaa gtcaaggaag atccatctaa gtactattgg actgagcaag atattcaaga
    44941 agggacgata gaaaaaccaa cacttgttac gcctacacga agtttttatt tgaacaaaga
    45001 gcatcaatta gttttgactt tttctcaata cgaaattgct cctggttata tgggtacacc
    45061 agaatttgtc attcctaagt ccgtgacaaa aacgattttg gcatcggaag attatttgga
    45121 tcattagacg agaagtagtt gcgactagca gtttcaattt tcaagtaagt acaagtaaat
    45181 aataagaaga gaaaaagaga aagaagagat atagtgaaaa gaaattattt agttatcagt
    45241 attattgctt tatccgtgct tttgtttttc gaaattactt cggtggcctc cttgatcacc
    45301 gaagtaaaag gacaaacaca ctcttcggag cattcgtcac aatctttcaa acaatcagaa
    45361 caatcaacca ttaaaagcca atcaactgaa acagctaacg tcaaagaagc cattcaagaa
    45421 tcagcggaaa aggtacaaag tcagtcatca caatcaacac aagacagtac aaaaacgagc
    45481 cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg agcgaccaca
    45541 acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta
    45601 ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc agggcacgag
    45661 attggcaatc acacatacga tcatcagaat ctggccactc tttctgccca gtcgatgacg
    45721 gaggaagtga cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac ccccactttt
    45781 gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc
    45841 atcgaatggt ctgttgattc agaagattgg aagacaagaa atcctgattt gattttgcag
    45901 aagatccaag caacagtata cgatggggca attattttat tccatgatat ctatcctgaa
    45961 acgattcgtg ccgttcctca agtgatcgat tatctgaaag agcaagggta tcgcatcacg
    46021 acagttgggg atttattagg gcatccaacg gctgtcgaga actattatgg cagaaacgat
    46081 catcggccag ttcagtaagt gagacaagta tctttaataa ataacacatt ttaataagta
    46141 gttgaacaca taactacctt acttgcaaag gcttaacaat atctatacag gtacaacaaa
    46201 aaactgtcca ccaattttct ttggcggaca gttttcactt aaaaataaag aagcaactat
    46261 tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca
    46321 agaaccggaa tcttacgtga tatccactac actaaggacg caagcactgg gataattgta
    46381 actaaacaga taaaaaatta caaggggatt ggacaaaaaa atgaaatttg agcaaaattt
    46441 ttcacaaaaa cagcagcaga cacagaaact agccatgacg caacagttgc aacagtccat
    46501 ccaagtttta caatataaca gtgaggagct gttggcattc gttgagaatc aagcaatgga
    46561 aaatccattg gtggaagttg ttgagccgga atggcagcca gattacataa aagcttcttc
    46621 atcttcttat gaaggagaag agacaaatta cctaaatcag attcctgata caaaagggtc
    46681 tttatttgat tcattgatcg aacaagtcca tttgaattac cgagatactt ttttgaggaa
    46741 aatcgttctt tatcttgtag aatacatcga tttgaacgga tttttgacga ttagtttaga
    46801 agaagccgga aaagagattg gagctacgcc tatccaaatg ctcgatgcgt tgactctgat
    46861 tcagcaatta gaaccagctg gagtaggtgc acgtagtttg caagaatgtc tcatgcttca
    46921 aacagaacga gatgattatg cgccggaatt agcttatatc gtgttggaag aatgttttga
    46981 ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg atctacatgc
    47041 tgttcagcag atattcgact atttacaaac gttgagtcca agccctggcc ggattttcga
    47101 ccgtagcagc gaattgtaca ttattcctga tgtccgggta ttagtggacg acgataaaaa
    47161 tgtacaagtt atctcaaacc gaaaaaatca gccaaatata cgctttcaag agagttactt
    47221 caaacaaatg tcacaacaag cagataaaga aactgaaaat tatttaaaag aacggaaaca
    47281 ggaattcgaa tggttgaaga aaacgatcct acaacgtggg gatacaattt tgcgggttgc
    47341 tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc
    47401 tcttacgtta aaggaagttg caactgagat agatgtgcat gaatccacgg tcagtcgtgc
    47461 agtcaacggg aaatatttgg aaacagactt cggtgtattt gaattaaaaa aatttttcac
    47521 tacacgaatt cctacgaata gtacagaaca gacagaagac ctttcagcag atacagcgaa
    47581 gaaaaagctg caggaattag tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa
    47641 attggttgaa ttgctgaaaa aagatgagat agcgatctca agacgaacag tagcaaaata
    47701 tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata actaatcagt
    47761 caaaagacta gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa acgagagcaa
    47821 aactgttaga cttacagtcg tgaggttgag atttcccttt attgatgtga aattcgaata
    47881 gttaatttct ttttttatcg tatatgggtc gttttaagtc tacattggac gtaaagtgtc
    47941 caactaagga gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt gcaccagata
    48001 ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa tatctattgg atgcagccga
    48061 ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga acgagtattg cgaacagaaa
    48121 cagatctttt gaaaaatctg aatttgatcg acacctcaaa aagcggtatg acgctgacaa
    48181 ctaagggcga agaagtttat caaagccttg aaaacttcat ggatcagctt ttaggtacgc
    48241 atcaaaccga acaacagttg gcagaatatt ttggtaacca acgctgtatc gttgtttcag
    48301 gaaacagcga agagcagacc aaagtggctg atgcattcgg cgaagcgctg agcgaagccc
    48361 tagaccgact acttccagaa ggcgagaata tcatcgctgt gatgggagga actactatgg
    48421 ctgtggtagc cgaacaattg tctaatctgg aaaataagaa acggcataac ctgtttgtcc
    48481 ctgcaagagg cgggattggt gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa
    48541 tggctgctaa agcaaacggt aaccaccgtg cactttatgt tccggaacaa ttaagtctgg
    48601 caacttacaa ttctttactc aatgagccat ctatccaaga agtcttaaac ttgattagtg
    48661 aagcgaattg tgttattcat agtattgggc gtgcattaca catggcagca cgtcgtaaga
    48721 tgactgaaaa agaattagtc atgctgaaac aagcaaatgc tgttgcggaa tcttttggct
    48781 actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc atagaattgc
    48841 aagatttgga aaaagtcccg atcatcatgg caatcgcggg cggaaaatca aaagccaaag
    48901 caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact gatgaagctg
    48961 ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa atattttttt gattttcata
    49021 aggaggaaat cttaaatgac agttaaagta ggtattaatg gttttggacg tattggacgt
    49081 ctagcattcc gtcgtatcca agatgtagat ggaatcgaag tagtagcaat caacgacttg
    49141 acagatgcta aaatgttggc acacttgtta aaatacgaca caactcaagg acgtttcaac
    49201 ggaacagttg aagttcatga aggttcattc aacgtaaacg gaaaagaagt taaagtatta
    49261 gctaaccgca acccagaaga attaccatgg ggcgaactag gcgtagatat cgttctagaa
    49321 tgtactggtt tcttcacttc taaatcagca gctgaaaaac atttaacagc tggtgctaaa
    49381 cgcgttgtta tctctgctcc tggcggaaac gatgttccaa caatcgttta taacacaaac
    49441 cacgaaacat taactggtaa agaaacagtt atctcaggtg cttcatgtac tactaactgt
    49501 ttagctccta tggctaaaac attgaacgac aaatttggtg ttgttgaagg attaatgaca
    49561 actatccacg cttacacagg tgaccaaatg actctagacg gacctcatcc taaaggtgac
    49621 ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta actcaacagg tgctgctaaa
    49681 gctatcggtt tagtaattcc tgaattgaac ggtaaattag acggagctgc tcaacgtgtt
    49741 cctgtaccaa caggttcatt aacagaatta gtaacagttc ttgaaaaaga agtaactgtt
    49801 gacgaaatca atgcagcaat gaaagaagct tcaaacgaat cttacggata caacacagac
    49861 gaaatcgttt cttcagatat cgttggtatg acttacggtt cattatttga tgctacacaa
    49921 actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac
    49981 gaaatgtcat acactgcaca attagtacgt actttagaat acttcgctaa cttataagat
    50041 agacaaacgt acaagtgtaa attatagaca tgtacattta aaagcgggga agcaatcgcg
    50101 cttcttccgc tttttttgat aataagttta ttcaggagga aataaaatgg ctaagaaaac
    50161 agtgaaagat atcgatttaa aagacaaaaa agttcttgtc cgtgttgact tcaacgttcc
    50221 tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa gcagcattgc caacaatcaa
    50281 ctacgttcta gaacaaggcg gaaaagcaat ccttttctct caccttggac gtgtaaaaac
    50341 agaagaagat aaagaaggaa aatctttagc tccagttgca aaacgtctag gcgaattatt
    50401 aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa caattagaag aagcaatccg
    50461 caacatgaat gacggcgacg ttgtcgtatt cgaaaataca cgatttgaag atattgacgg
    50521 taaaaaagaa agcaaaaatg atacagaact aggtaaatac tgggcttcat taggcgatgt
    50581 atttgttaac gatgctttcg gtacagctca ccgtgcacat gcttctaacg taggtatcgc
    50641 ttcaactggt atcccaacag ttgctggatt cttgatggaa aaagaaatca agttcatcgg
    50701 tgaagcagta gaagaaccaa aacgaccaat gattgcaatc cttggtggcg caaaagtttc
    50761 tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca gataaaatct tgatcggtgg
    50821 cggaatgact tacacattct acgaagcaaa aggaatcaag atcggtaact ctctagtaga
    50881 agctgataaa gtggaactag caaaagaatt aatcgaaaaa gcaggcgaca aacttgtctt
    50941 gccaatcgac aatgtctgtg caccagaatt ttcaaatgat gttgaaacac aagtaatcga
    51001 aggagacatt cctgatggtt tgatggcttt agacatcggc ccagcatcag taaaattatt
    51061 tgctgataca ttacaaggtg caaaaacagt tgtttggaac ggacctatgg gtgtatttga
    51121 aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa gcaatcgcta acttagaagg
    51181 cgcaacaaca atcatcggtg gcggagactc agccgcagca gcagaacaat taggatttgc
    51241 tgacaaattc acacacatct caacaggtgg cggcgcaagc ttggaattgc ttgaaggtaa
    51301 agaattacca ggactagcag caatcaacga taaataaacg accaagtttg ctcaattctg
    51361 acgagtaatc gatcaaaagg tgaagggagt tttcatgact ccctagccat ttttcaataa
    51421 attaaggagt gtgttcctca atgcgtaaac ctattatcgc aggtaactgg aaaatgaaca
    51481 aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa aaacaaaatc ccttcaaatg
    51541 acaaagttga ttcagtcatc ggatctcctg cattattctt acaagaatta atgacatcag
    51601 ctgaaggtac agaactaaaa atctcagcac aaaactgcta ctgggaaaac tcaggtgcgt
    51661 tcactggtga aacttcacca gcagctcttg ctgatctagg tgttcattat gtgatcatcg
    51721 gacattcaga acgtcgtgaa tatttccatg aaacagacga agacatcaat aaaaaagcga
    51781 aagctatctt tgcaaatggt atgacaccaa tcctatgctg tggcgaatct ttagaaacct
    51841 atgaagcagg caaaactgct gaatggatcg aaggacaaat tacagctggt ttagctgatc
    51901 tttcagctga acaagtgtca aacatggtta ttgcttatga accaatctgg gcaatcggta
    51961 ctggtaaatc agctgacgca caaatcgctg acgaaatttg tggagttgta cgtcaaacag
    52021 ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg tatccaatac ggcggttctg
    52081 taaaaccaga aaatattgct gaatacatgg caaaagaaaa tgttgacggt gctttagtcg
    52141 gtggcgcaag cttgcaagca gattcattct tgtcattgtt agacgctgta aaataattgt
    52201 tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg agcaaaatta actaaaatcg
    52261 tatatgaggg aaatccctta ggaaaaaaca aactcaaagg agagacaaaa catgtcaatt
    52321 attactgatg tttacgctcg cgagatctta gactcacgtg gtaacccaac aatcgaagta
    52381 gaagtataca cagaaagcgg agcttttggc cgcggaatgg ttccttcagg tgcttcaact
    52441 ggtgaatacg aagcagttga attacgtgac ggagacaaag ctcgttatgg cggtaaaggt
    52501 gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg aagcaatcat tggctacgat
    52561 gttcgtgacc aaatggctat cgacaaagct atgatcgctt tagacggaac tcctaacaaa
    52621 ggtaaattag gtgcgaacgc tattttaggt gtttctatcg ctgttgcacg tgcagctgct
    52681 gactacctag aagtaccttt ataccactac ctaggcggat tcaacacaaa agtattgcca
    52741 acaccaatga tgaacatcat caacggcgga tcacatgctg ataactcaat cgacttccaa
    52801 gaattcatga tcatgcctgt tggcgctcca acattcaaag aagctctacg tatgggtgct
    52861 gaagtattcc acgcattagc ttcaatcttg aaagctcgcg gattagcaac ttctgttggt
    52921 gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag aaggttttga agtaatcatc
    52981 gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg ttgttcttgc tatggatgct
    53041 gcttcttcag aattctacga caaagaaaaa ggtgtttacg tactagctga ttcaggcgaa
    53101 ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag aattagtttc taaatatcca
    53161 atcatctcaa tcgaagacgg attagacgaa aacgactggg acggattcaa gaaattaact
    53221 gacgtattag gcgacaaagt tcaattagtt ggtgacgact tgttcgtaac aaacactcaa
    53281 aaattatctg aaggtatcga aaaaggaatt gctaactcaa tccttatcaa agtgaaccaa
    53341 atcggtacat taacagaaac tttcgaagct atcgaaatgg ctaaagaagc tggctacaca
    53401 gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa caatctctga tatcgctgtt
    53461 gcaacaaacg ctggccaaat caaaactggt tctctatcac gtactgaccg tattgctaaa
    53521 tacaaccaat tactacgtat cgaagaccaa cttggtgaag ttgcagaata caaaggtttg
    53581 aaatctttct acaacttaaa aaacaaataa ttcttttcta atgaagatac atgatcgact
    53641 attgaaagat agtcgagtat gatcacttag ttaagtaatc gaactctcta cttgctgaaa
    53701 tatcagcagg tagagagttt tttttgcaac tagcttctgt atatgatgtt tacaaagaat
    53761 ggtacaagaa aagatcatcc ttgaaaaagg aaagctaaca ataactagca tcatataaga
    53821 gcttttaagg atagaacagt agaataggaa tctatgagca agtcctctcg tataaataaa
    53881 ataggaggga agtagcagca tatttttcat aaagtggctc tctttcttca tttttacttt
    53941 ggttgctcct atttgaaaat tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga
    54001 gaggattaga tgaaaaaaga agaaaaaatc gcgattttac aagagatcat ccgaattaaa
    54061 tcagtgaatg gaaacgaagg tgaagtagca gcctatttga ataagctttt ggcaaagcat
    54121 gacataacag gagagattgt ttcttacaga gatggtagag ataatttgat tgctcgttac
    54181 caaaagggcc aatcaggaaa agtgttaggc ctatctgggc atatggatgt cgtggcggct
    54241 ggtgatgaat cgtcttggac ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg
    54301 tacggacgtg gtgctacaga tatgaagagt ggcttggcag caatggtgat tgccatgatc
    54361 gaactgaaag agtcaggaaa accatttaac ggaacagtca aattattagc cactgtcgga
    54421 gaagaagtgg gtgaactagg tggggaagag ctcacgaaag caggctatgt agatgactta
    54481 gatgccttga tcatcggaga gccaactaat tacagtttga tgtatacgca catgggatca
    54541 atcaactata cagtcacctc gcacggaaaa gaagcgcata gctctatgcc agaccaaggt
    54601 tataatgcaa tcaatcacct aaatgaattc attacgaaag caaatgctga aatgaatcac
    54661 ctcgctgaaa caatcgagaa tcctgtccta ggaaaaacaa tccataatgt tacattgatc
    54721 agtggcggga atcaagtcaa cagcatccca agccatgcgc agctccaagg gaacattcga
    54781 tcgattccag aatacccaaa tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa
    54841 ttgaatcaag agacagacta tcatttggaa ttgatgatcg attacaacaa aatacctgtc
    54901 aaagctgatc cagattctcc tttgattcat tgcattcagc aacaattcag ccagccatta
    54961 cctttagttg gagcggcagc aacaacggat gcagccgaat ttacgaaagc taaccattcc
    55021 ttcgactttg tcgtctttgg gcctggtgtc gttactttgc ctcatcaagt agatgagtat
    55081 gttgaaatcg ataattattt ggatatgatt gaaaaatatc aaggaattat tttatcctac
    55141 ttagcgtaag atttttttaa taaatgaaaa aacagagcca gattagcatt attttgctaa
    55201 tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt gtggcaacat
    55261 agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt ctgtacctgc
    55321 ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata gctgaacgat
    55381 catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga tcgaagaaac
    55441 gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc tgtttttatc
    55501 tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa tttggattgg
    55561 cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc tggacaatcc
    55621 tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc cgatagaatc
    55681 gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat tgatcccaat
    55741 acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca ttttgttagc
    55801 ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt aaaagaacgg
    55861 ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta ctgttagcag
    55921 gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt ccttgagttt
    55981 ccacagcagt gaagcttttg aaaccttcac agatggcccc ctcgataaaa taagcaacag
    56041 ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg aagtcaaaat
    56101 aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga cagcagtatt
    56161 cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc atttctatcg
    56221 tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag taattgtatt
    56281 cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag acaaaacgat
    56341 ccatatgttt cccaatattt gggttgactt tcaaagcaaa agcgatattg gtcaaaggac
    56401 ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa aaatcaacgg
    56461 catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc cctaaaccat
    56521 ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt tccattcctt
    56581 gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca ttctgcgtac
    56641 ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg atatcagggg
    56701 atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa tcaataatta
    56761 cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta ttctgggaag
    56821 tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag tatactggct
    56881 tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac tttaagtaaa
    56941 tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc ctatggatcg
    57001 gtacattgga taatagttag attatagttt ggcagaaagc ggcgggtgta caatggttat
    57061 tttttatttc attcttattc tgataattat agggatcagt taccattttc tttcttattt
    57121 ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt atgaagacac
    57181 tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac gacaaaaatt
    57241 agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca aaagttatga
    57301 cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata aatgggtcat
    57361 ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag gtaaaagata
    57421 tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag aaagtgaagg
    57481 agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga tccaactcat
    57541 cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg gagcttctac
    57601 gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta ttttagacag
    57661 cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca ctgtctttcc
    57721 taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg gttactcctt
    57781 aaagcaagca tcagctacga gacagcttgg cagcaaccat ctgcctttat tggtcattca
    57841 tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa atgctacggc
    57901 aggggataaa gcactgctgc ttgttccaga agcagaacac ttggaagcat cgatgaaaga
    57961 tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa cgatgaatta
    58021 attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag atcaggagac
    58081 tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg catatggaaa
    58141 tcaaagaaga aaaaaatcgt cttgctttat tcaatgatga acaacaagag atcggagaaa
    58201 tgacatggtc agatgcgggc ccagacatca tgatcattga ccatacattt gtagatccag
    58261 catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa ttagcaagac
    58321 gagacgggaa aaagatcatt cctttatgca cttatgcaaa aaaagagttt gaaaagaaac
    58381 cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt ttgaactttc
    58441 agctgttatt ccatttatat agagttgtca cggactttta tctatgataa agttaaagag
    58501 acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga atcgtgatcg
    58561 ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa aatagtgcag
    58621 cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct cgcggctttg
    58681 aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc ttattatttg
    58741 tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg cgggcggctt
    58801 tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg taaaatgagg
    58861 cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa catggaaagc
    58921 gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga atgctcgcac
    58981 gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga cacggaacaa
    59041 tggagccata tgatattttg caagaaaaag cagaaagctg gtgggcagac actaaaaaag
    59101 ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga ttatcaatgg
    59161 gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc ggttttttct
    59221 gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa tacgtacgtc
    59281 aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca gttgcttacc
    59341 gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc gtagaaagta
    59401 ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat gaaatgatcg
    59461 accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg acacttcgat
    59521 ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa ttagaaaaag
    59581 acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga caaaagaaac
    59641 gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt tttctatgga
    59701 agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac tagtacaaac
    59761 gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa aagtgaaact
    59821 tcctttaaaa ccaatcttga tcgaaggaac atttcgagca aatgaacgag gatttggatt
    59881 tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga ctggttttgc
    59941 aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag atatggatcg
    60001 cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt tagcagggga
    60061 attcgttgct tattcagaag aagaaataca agaaacggat ctttacggcg ttgttatccc
    60121 gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag ttcgtccagt
    60181 tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt atgcgactag
    60241 tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca tggatatcct
    60301 ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat tgtctgaagc
    60361 agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag atttaagaaa
    60421 tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg cagttacggt
    60481 taaaaaatta gataacggta attattttct tggagtacac attgcagatg tttcttacta
    60541 tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa gtgtatattt
    60601 gactgatcga gtgattccga tgatcccgca acgtttatcg aatggtatct gttcactgaa
    60661 tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa atggacaagt
    60721 agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga cctacacagc
    60781 tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg ctcagctcgt
    60841 acctatgttc caagaaatgg gtgaattgca tcgaatcctt gaaacgatgc gaatcaaacg
    60901 tggcgcgatt tcttttgagg atcgcgaagc aaaaatcctt gttgatgctg aaggacaccc
    60961 aaatgatatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat ctttcatgct
    61021 tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt ttatttaccg
    61081 aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg cttcagcttt
    61141 gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac agcatgtgat
    61201 cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc taagaagcat
    61261 gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg cagattacta
    61321 cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc gtctgatccg
    61381 ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctacgg aacttcctga
    61441 aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac gtgaagtaga
    61501 ttccatgaag aaagcagaat tcatgatgga caaagtgggg gaagaattcg atgggatcat
    61561 cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag agggattgat
    61621 ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt tagctcttgt
    61681 aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa aagtagtcaa
    61741 agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag aggttacagc
    61801 gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa aacaagcagg
    61861 aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta agcaaacgtt
    61921 cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga aaaagaaaaa
    61981 ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa ttgcacaaaa
    62041 caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga tggtcttgca
    62101 aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg gatttattcg
    62161 cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg aacaaggaaa
    62221 tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa aacaaatcat
    62281 ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa aagtctatat
    62341 tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat cttatgataa
    62401 acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat taaaaaattt
    62461 ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt acttaatgat
    62521 aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt cttagatcag
    62581 gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa agtgtctaat
    62641 tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg tggttagaaa
    62701 ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc ggggaaaaga
    62761 ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt tggtttgatg
    62821 gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta gcttactttt
    62881 ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa tgggtcattg
    62941 attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc aatttccatt
    63001 tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt ttagtaacaa
    63061 aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac ccgtttgtta
    63121 ctttaacttt ggcatttata atgatcttac ttactcactt atttggggtg aaaaagttag
    63181 gcttcaaagg atatttcgta aattcgttct tgaaaccata tagttttatg tttccgatga
    63241 aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat gggaatattt
    63301 atgcggggga agttttgcta accttgattg caaaaatggt aataagctta ggctggttct
    63361 cattgccgtt agcaattcca ttagagatgg tctggatcgc attctccttg tttatcggaa
    63421 gcatccaagc atttgtcttc gttactttat ctatggttta tatgagccat aagattgaag
    63481 tggaagaata atattcgttt taaaaacaaa ctactattta tctaatttag gaggaaaaca
    63541 attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg agccggttat
    63601 ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc tgaaatgtct
    63661 ggtcaattcc gtacaacaat gtttatcggg gtagctctag tcgaagctgt tcctatctta
    63721 ggggtagttg tcgccttgat tctagttttt ggtgtttaat tgaatcagta ttaaggcaga
    63781 aacaatttgg tttgttactg cttttttaca tgaaaactag aagtacttca aagaaaggaa
    63841 ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat gttaggaaat
    63901 gtcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca ttttgcttgg
    63961 ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga tttggattct
    64021 gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca acaattgtta
    64081 gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg agaattaagc
    64141 cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag taaagcccaa
    64201 gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga tgttgcagaa
    64261 ctatctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga aatgcacgaa
    64321 tcattaatca atcaatatat cgaaggtcta ggttcttcaa atgaaactag ataaatatac
    64381 agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa ataaagcaga
    64441 agaaatttat caagatctcg taagcttacg tcaaatctat gaacaagtac ctggattagg
    64501 taatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca tggacaagct
    64561 tgttagtggc tatgatggaa tcgttaaaaa ctttttagaa gttgtttatg cgtataaccg
    64621 aatggatgat cttttgttca tgatcgaaga atatgaacgt cgatatgatg aacatcaagg
    64681 tttgctgttg ggaagtgtca caacagcagt tccactttca gacgaacaac gaatacaatt
    64741 agaaaagaat gttgcaaaaa caatgaacta tcaatcagtc gaattaaaac aaatcgttga
    64801 ttcttctatt attggtggcg cgattgttga agctaaccat cgagtaatcg acggaagcat
    64861 tcgcacgcaa ttagaaaata tgcgtaatca attaaataga tagtacaaag aggtaggtga
    64921 atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa attgaaaatt
    64981 atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt gacgggatcg
    65041 ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt tcaaatggct
    65101 catacggaat ggcgcaaaac ttagaatcaa atgatgtagg tatcattatc cttggcgatt
    65161 tcgagtccat tcgtgaagga gataaagtaa aacgaacagg aaagatcatg gaagttccag
    65221 ttggagaggc cttgattgga cgggtagtta atccgctagg tcagccaatt gacggactag
    65281 gtgaaatcgt tactgacaaa gctcgtccag tagaagcaat ggcaccaggc gttatgcaac
    65341 gtaaatctgt taacgaacca atgcaaacag gtctaaaagc aatcgacgct cttgtaccaa
    65401 tcggacgcgg ccaacgtgaa ttagttatcg gtgaccgtaa aacagggaaa acttccattg
    65461 cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat gtagcaatcg
    65521 gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat ggcgcaatgg
    65581 attacacaat cgttgtgaat gccggtgcgt ctcaaccagc gccgttgctt tatatcgcac
    65641 catatgctgg tactgcaatg ggtgaagagt tcatgtacaa cgggaaacat gtcttgatca
    65701 tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg ctgcttcgcc
    65761 gtccaccagg tcgtgaagct tatccagggg atgttttcta cttacactca cgcttattgg
    65821 aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc ttaccatttg
    65881 ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt tcgatcactg
    65941 atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca gcggttgatg
    66001 ccggactatc tgtatctcgt gtagggggtt ctgcgcaaat caaagcaatg aaaaaagttg
    66061 ctgggacttt acgtctagac ttagcaagtt accgtgaatt agaagcattt acacaattcg
    66121 gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt acagtagaaa
    66181 tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg attttgtatg
    66241 ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac ttcgaacatg
    66301 aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc cgtacaacaa
    66361 aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa gacatgttct
    66421 tagctacaaa aggaaataat tcctctacag aagataaatt gaaatccatt caaaatgcat
    66481 agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat cgcttcaacg
    66541 aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa gttgacaaaa
    66601 tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga aatcgttacg
    66661 catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg ggatatcaat
    66721 tacaacagca tgttgctcag ccgtcctgtt aagaaaacgg gttatatcgt catcactgca
    66781 gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat gtctatttta
    66841 gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg aactggtgca
    66901 gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct atctgatcag
    66961 ccaagctttg atgaagtccg caaaatcgta aatatggcaa caactatgta ccaaaacgaa
    67021 gtattcgatg aattatacgt atgttataac catcatatca attcgctaac tagtcagttt
    67081 cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac tactttcgaa
    67141 caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt gcctcagtat
    67201 gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca tgctgctggt
    67261 atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga gctgacggtt
    67321 tcttacaacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat tgttgccgga
    67381 gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag gcaagattgt
    67441 tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct taccagatat
    67501 caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg ttcttgaagc
    67561 agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa cagatggttt
    67621 acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg taggtaaaga
    67681 aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa cacctttccc
    67741 agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg aattaagtac
    67801 aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc catatttaaa
    67861 aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac tgatccaaga
    67921 actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg gtgtaggtga
    67981 acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag ttatcgaaaa
    68041 aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc gtgttgcttt
    68101 gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg tactgttgtt
    68161 tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct tgttaggacg
    68221 gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc aattgcaaga
    68281 acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct atgtgccagc
    68341 cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg cgacaaccaa
    68401 cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat tggcttcttc
    68461 ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag caacagaagt
    68521 ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct taggtatgga
    68581 tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc aattcttctt
    68641 gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat atgtccctgt
    68701 agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg atcttcctga
    68761 agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga aaacactagg
    68821 ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta actcctgccg
    68881 gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct ggtgagcttg
    68941 ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa gttcgtgtaa
    69001 aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga atcatggaag
    69061 ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt gatatcgatg
    69121 tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa gcaaaagcaa
    69181 aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca atcaatcgta
    69241 tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt taagaacagt
    69301 aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt tacgaaaatt
    69361 gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt ctggaacacc
    69421 gtatatcgga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac aaatggaaaa
    69481 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga ttgaacaccg
    69541 tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt ttgcatattt
    69601 ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta tacttgtttt
    69661 tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca atcgttagaa
    69721 aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg agtcatttgg
    69781 cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact tttttcagac
    69841 caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt ttagggtaca
    69901 ctgcaagtaa ttttttctta gaagttatcg ctttatgccg aaatcttttc gttagcctgt
    69961 aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa agattttgag
    70021 taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc atcgtaagag
    70081 gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct gttttgccta
    70141 ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac gttccgattc
    70201 tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat gtagattttg
    70261 atgaacaaaa gaatcaagta acaatcgatg cttcaagaca attagaaatc gaagcgcctt
    70321 atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta cttgccagaa
    70381 atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga ccaatcgatt
    70441 tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac ggctatatcg
    70501 aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct agtgtaggtg
    70561 ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc attgaaaacg
    70621 tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg ggcgctcaag
    70681 tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta catgcagtca
    70741 accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct gctgcaatga
    70801 cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca ttgatttcta
    70861 aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt gtcatcggac
    70921 caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc cctacagata
    70981 tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgta gtgactgaaa
    71041 cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac gctcatgtca
    71101 aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt gcagaagtat
    71161 atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa gcaaatggaa
    71221 tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc catatcaaac
    71281 tacagcaatt aggtgcagac gttgaacgtg tagacatgga tcaaacttct gctgaaaaaa
    71341 cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat atcttattaa
    71401 ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg gccggaacga
    71461 tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag ccaagtttgt
    71521 ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt ctagtcgata
    71581 tgaagtgagc catgaagcag aacatgacaa aagaaaacag aagggaacgt tgacgttagt
    71641 cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga agatattgat
    71701 ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta gctgccgtta
    71761 ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg cactaaaagg
    71821 gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag gcggtatcga
    71881 ttatgtacca ttgacattcc aattaacgcg aaataagaaa gataaaaaag aaccagatta
    71941 taaaaaggtt gtgaaaaata cgagtagcac tgagcgataa agaaacaatt tggaaaatag
    72001 cttcttatat tttgcaaaat tgtgagttat tgccgaagtg ttggattttg aacaccgtca
    72061 aaaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag gaaaattatt
    72121 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
    72181 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
    72241 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
    72301 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag tagccccaac
    72361 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
    72421 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
    72481 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
    72541 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag taattttagc
    72601 aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa aatgctttca
    72661 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
    72721 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
    72781 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
    72841 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
    72901 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
    72961 tcatggattt aatattttcc gagatttgga aactctgttt caactcccag taacgattga
    73021 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gtgattatca
    73081 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
    73141 aatcaataaa ggggcacatc tttatggtgg agaatttggg ttgatgttct taaatggcga
    73201 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
    73261 aggtctcgat aaaaattctt ataccggaaa agatgtcttt gaattagcag aaactggaga
    73321 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
    73381 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggtgttt ctgcaaaaga
    73441 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
    73501 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
    73561 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
    73621 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
    73681 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa ataaagatgt
    73741 tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt aagaattatg aagtcactca
    73801 gttatttata gggaaaaaaa tgattggtga gatcttagcc tatgggccaa aagaatatga
    73861 aattttcttt ggtgaagaag attttggaaa agaaaaaagt cttgaaaatg cgatagaaac
    73921 agtgattcga cactggaatc tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg
    73981 gttgcaaaaa tctggaaaat taggtattat aactaagtca ggttgataca aacctttcgg
    74041 aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc gttccttagg gttcagtggt
    74101 tcgaatccac tctcctccat ttcattgggg tatagccaag cggtaaggca agggactttg
    74161 actccctcat gcgttggttc gaatccagct accccagtat accacaatcg taagctctcg
    74221 ttagttcgag agctcttttt tttgctttgc taccttgttc cttgaagatt ctgctgattt
    74281 aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg aaagaaaaag
    74341 tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac aatattgaag
    74401 tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc ccaacgaatc
    74461 atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat aagtcggcat
    74521 tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa aaaattgttt
    74581 ctgacaatag gagtaggaag aacgatgtgt gtctaaatcc tctgttgatg gatagaaacc
    74641 tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa acgaaaggac
    74701 gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac caattaaaaa
    74761 atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa agagaattag
    74821 cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac ttggcagatg
    74881 aaggaatctt agaaagaaag atcggttcgg gtacatatgt tgcgcgagaa aaagtacaag
    74941 aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcgcaaaat cgagtaccgt
    75001 ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg gaaaaactaa
    75061 acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca gatgatgtcc
    75121 ctatttgttt tgaagttgcc agtgtgccac aaaagctgat cgatggctac agcaaagcag
    75181 agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag atcggtccag
    75241 cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat ttagaggtaa
    75301 aacgaggaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac ggtacgccgt
    75361 ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg gaaaaataaa
    75421 aaacaggtga tccctgattc cttacttaga aataaaagag aatcagaatc acctgttttt
    75481 atatatgatc agttaccagt tgctcgtgat aagaaacagg accctcttca aattggactt
    75541 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
    75601 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
    75661 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
    75721 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagacagca tgagtgtatg
    75781 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
    75841 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgcca gccgttccac
    75901 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
    75961 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
    76021 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
    76081 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
    76141 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
    76201 caatcgctgt agtatgcgat aataaagaca aaggtaggtg gatctgggga tgcaggaacg
    76261 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
    76321 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
    76381 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
    76441 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
    76501 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
    76561 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcgtagt
    76621 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
    76681 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
    76741 gactcggata ttttcgctgt gtccgcttct gacgcagata acaatcttgc aaataaagat
    76801 gtagatgtac tgttattagg acctcaagtt cgctttatga aagctgattt tgaaaaacgt
    76861 ctggaaccaa aaggcattcc tttagacgtc atcaatatgg ctgattatgg tatgatgaat
    76921 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
    76981 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
    77041 ataatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattaaa gcaatagact
    77101 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
    77161 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
    77221 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
    77281 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
    77341 ttactgaaaa acaaacggaa atcgcaaaga gaatactttg ccattcagaa aaaagacatc
    77401 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
    77461 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
    77521 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
    77581 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
    77641 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
    77701 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
    77761 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
    77821 acaaacttgc tttgcgcttt catcggcgtt tacttcctga acccgtcttg gttttcatga
    77881 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
    77941 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
    78001 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
    78061 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gacatactgt
    78121 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
    78181 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
    78241 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
    78301 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
    78361 gtgcagttgc tgcagtaaag atatcgtcca aaatttaaca ttcagcgaga ttattggtgt
    78421 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
    78481 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
    78541 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
    78601 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
    78661 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
    78721 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
    78781 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
    78841 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
    78901 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
    78961 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
    79021 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttagcaa gatgaattag
    79081 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
    79141 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
    79201 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
    79261 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
    79321 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
    79381 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
    79441 tggttgttga caaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
    79501 cccgtgaaac gggcatgaat actgcagatg ctactgcttt attcaatgaa gaggacgaaa
    79561 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
    79621 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
    79681 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
    79741 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
    79801 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
    79861 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
    79921 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
    79981 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
    80041 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
    80101 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
    80161 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccaca
    80221 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
    80281 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
    80341 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
    80401 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
    80461 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
    80521 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
    80581 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
    80641 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
    80701 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
    80761 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
    80821 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
    80881 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
    80941 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
    81001 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
    81061 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
    81121 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
    81181 tcgtccagat ttgctttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
    81241 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
    81301 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
    81361 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
    81421 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
    81481 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
    81541 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca cagttctact tgtcattgga
    81601 agatgatttg atgaaacgat tcggttcaga acgaatcaaa tctttcttag atcggatgaa
    81661 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
    81721 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
    81781 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
    81841 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
    81901 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
    81961 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacagctca
    82021 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
    82081 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
    82141 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
    82201 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
    82261 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
    82321 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
    82381 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
    82441 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
    82501 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
    82561 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
    82621 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
    82681 aaggtcacaa gcttcaggag gtctctttga cttagaccag ctggaagaag acattgcgga
    82741 agcagaagca aaaatggctg aacctggttt ttgggatgat agcgaagctg ctcaaaaagt
    82801 gatcagtgaa aacaatgcaa ataaagaaac ttatgatcaa ttccattctc ttgctgaaga
    82861 actggaagaa atcgaattga tggtggagat gttgcaggaa gaacctgatg cagccatgca
    82921 agaagaagca gaaacacgta tcaatgcgct agatgaaaaa atgaagacat atgaattatc
    82981 tatgttgtta gatggtcctt atgatcgaaa caacgccatc gttgaattgc atccaggggc
    83041 tggcggaacg gagtcccaag attggggcag tatgctttta cgtatgtata cacgctgggc
    83101 agagcagcat gggtatcaag tagagacact agattatcaa gcaggtgatg aagcaggaat
    83161 caagagtgtg accctactga tcaaaggaca caacgcattt ggttatctga aatcagaaaa
    83221 aggtgttcat cgccttgtac gtatctcgcc ttttgactca gcgaaacgac gtcatacttc
    83281 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
    83341 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
    83401 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
    83461 acaacgttca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
    83521 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
    83581 aatcggctgg ggttcacaga tccgttctta tgttttccat ccatattcta tggtaaaaga
    83641 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
    83701 atttattgac ggctatttaa aaatgcatct acaataagaa cccattgtta aattgaaata
    83761 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
    83821 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
    83881 ggtacgaccg ctatccgcaa tctctcaatt gtcatcgaac aaggagagtt tgtctatgtg
    83941 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
    84001 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
    84061 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
    84121 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgggat
    84181 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    84241 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatagtg
    84301 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    84361 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    84421 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    84481 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    84541 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    84601 cagtgcggta acgatcacat tgactttagt cggtgtattc atggcagtta tcatgaacgc
    84661 aactaaatta gctcaagata tagaaggaaa cgttgatgtc tctgtattcg tagatatcgg
    84721 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    84781 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    84841 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    84901 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    84961 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    85021 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    85081 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    85141 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    85201 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    85261 caatccatca ttattacgtt cccattattc attgattcat ccacaaaata tcgtttggaa
    85321 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    85381 tatgagacgt ttcttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    85441 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    85501 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    85561 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    85621 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatgggtg ctcgttttga
    85681 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    85741 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    85801 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    85861 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    85921 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    85981 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    86041 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    86101 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    86161 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    86221 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    86281 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    86341 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    86401 atacaaaaga agtaggctat tctcccaaat tttttccgtc cattcactct actggtgata
    86461 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    86521 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    86581 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    86641 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    86701 cagagctaat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    86761 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    86821 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    86881 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    86941 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    87001 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    87061 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    87121 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    87181 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    87241 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    87301 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    87361 tcttaccaat aataggatta gctattcttt tatcaggctg ttcaaagtgg attgatcgag
    87421 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    87481 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    87541 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    87601 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    87661 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    87721 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    87781 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    87841 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    87901 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    87961 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    88021 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    88081 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    88141 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    88201 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    88261 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    88321 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    88381 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    88441 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    88501 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    88561 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    88621 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    88681 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    88741 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gactgggttt ggtatcttag
    88801 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    88861 cattaaaggc agtgccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    88921 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    88981 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    89041 cagcattgat gccaacaggt cttactactc ctgcttctac attgacgtcc atcttaacaa
    89101 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    89161 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    89221 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    89281 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    89341 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg
    89401 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    89461 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    89521 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    89581 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    89641 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    89701 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    89761 aacagtgata aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    89821 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    89881 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    89941 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    90001 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg attattgctg ttttactgtt
    90061 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    90121 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    90181 gagaaagaag tagcactata tacggatgat ctacatgtct ggtatggcga caatgaagca
    90241 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    90301 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    90361 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    90421 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    90481 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    90541 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    90601 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgtattgct
    90661 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    90721 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    90781 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    90841 tatttaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    90901 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    90961 aaagagatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    91021 gggatcgatc taagcttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    91081 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    91141 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    91201 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    91261 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    91321 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    91381 cataaaagtg ctttatcttt atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt
    91441 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    91501 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    91561 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    91621 ggtcacttga tcgaatacaa tgatacgaag aagatattca tgaatccaga aaaacaagag
    91681 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    91741 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    91801 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    91861 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    91921 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    91981 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    92041 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    92101 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    92161 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    92221 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    92281 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    92341 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    92401 taaactgaag ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg
    92461 tgaaaaagct gttctgcatc aagtaataag aacagtcaaa caaaaaatag ctcctcatat
    92521 ttttcgtttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggga
    92581 aagggtttgt gacaaaacat gtcacaaacc c
//
LOCUS       DAFBQW010000006        78259 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFBQW010000006 DAFBQW010000000
VERSION     DAFBQW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.006"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1087
                     /locus_tag="LK889_000578"
     CDS             <1..1087
                     /locus_tag="LK889_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5094838.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="LK889_000579"
     CDS             complement(1305..2447)
                     /locus_tag="LK889_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5094839.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="LK889_000580"
     CDS             complement(2452..2637)
                     /locus_tag="LK889_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK5094840.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="LK889_000581"
     CDS             complement(2991..3236)
                     /locus_tag="LK889_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094841.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="LK889_000582"
     CDS             complement(3230..3631)
                     /locus_tag="LK889_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5094842.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="LK889_000583"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="LK889_000583"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBK5094843.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="LK889_000584"
     CDS             complement(5649..7415)
                     /locus_tag="LK889_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBK5094844.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="LK889_000585"
     CDS             complement(7428..8222)
                     /locus_tag="LK889_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK5094845.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="LK889_000586"
     CDS             complement(8232..9608)
                     /locus_tag="LK889_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5094846.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="LK889_000587"
     CDS             complement(9728..10375)
                     /locus_tag="LK889_000587"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBK5094847.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="LK889_000588"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="LK889_000588"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK5094848.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="LK889_000589"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="LK889_000589"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK5094849.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="LK889_000590"
     CDS             complement(13042..14673)
                     /locus_tag="LK889_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK5094850.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="LK889_000591"
     CDS             14977..15648
                     /locus_tag="LK889_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5094851.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16373..16708)
                     /locus_tag="LK889_000592"
                     /pseudo
     CDS             complement(16373..16708)
                     /locus_tag="LK889_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16805..17257)
                     /locus_tag="LK889_000593"
     CDS             complement(16805..17257)
                     /locus_tag="LK889_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094852.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17264..17812)
                     /locus_tag="LK889_000594"
     CDS             complement(17264..17812)
                     /locus_tag="LK889_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5094853.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17857..18189)
                     /locus_tag="LK889_000595"
     CDS             complement(17857..18189)
                     /locus_tag="LK889_000595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094854.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18203..18460)
                     /locus_tag="LK889_000596"
     CDS             complement(18203..18460)
                     /locus_tag="LK889_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094855.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRANWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18655..19197)
                     /locus_tag="LK889_000597"
     CDS             complement(18655..19197)
                     /locus_tag="LK889_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094856.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19218..20309)
                     /locus_tag="LK889_000598"
     CDS             complement(19218..20309)
                     /locus_tag="LK889_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK5094857.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20317..22527)
                     /locus_tag="LK889_000599"
     CDS             complement(20317..22527)
                     /locus_tag="LK889_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094858.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22543..25071)
                     /locus_tag="LK889_000600"
     CDS             complement(22543..25071)
                     /locus_tag="LK889_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5094859.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25123..25533)
                     /locus_tag="LK889_000601"
     CDS             complement(25123..25533)
                     /locus_tag="LK889_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094860.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25546..25764)
                     /locus_tag="LK889_000602"
     CDS             complement(25546..25764)
                     /locus_tag="LK889_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094861.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25761..26753)
                     /locus_tag="LK889_000603"
     CDS             complement(25761..26753)
                     /locus_tag="LK889_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5094862.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26832..27743)
                     /locus_tag="LK889_000604"
     CDS             complement(26832..27743)
                     /locus_tag="LK889_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5094863.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27762..28106)
                     /locus_tag="LK889_000605"
     CDS             complement(27762..28106)
                     /locus_tag="LK889_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094864.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28126..28682)
                     /locus_tag="LK889_000606"
                     /pseudo
     CDS             complement(28126..28682)
                     /locus_tag="LK889_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28763..29263)
                     /locus_tag="LK889_000607"
     CDS             complement(28763..29263)
                     /locus_tag="LK889_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094865.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29264..29809)
                     /locus_tag="LK889_000608"
     CDS             complement(29264..29809)
                     /locus_tag="LK889_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094866.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29806..31062)
                     /locus_tag="LK889_000609"
     CDS             complement(29806..31062)
                     /locus_tag="LK889_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5094867.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31423..31734)
                     /locus_tag="LK889_000610"
     CDS             complement(31423..31734)
                     /locus_tag="LK889_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094868.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31757..33067)
                     /locus_tag="LK889_000611"
     CDS             complement(31757..33067)
                     /locus_tag="LK889_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBK5094869.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33681..34133)
                     /locus_tag="LK889_000612"
     CDS             complement(33681..34133)
                     /locus_tag="LK889_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094870.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34135..34500)
                     /locus_tag="LK889_000613"
     CDS             complement(34135..34500)
                     /locus_tag="LK889_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094871.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34599..38882)
                     /locus_tag="LK889_000614"
     CDS             complement(34599..38882)
                     /locus_tag="LK889_000614"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBK5094872.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39029..39268)
                     /locus_tag="LK889_000615"
     CDS             complement(39029..39268)
                     /locus_tag="LK889_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094873.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39358..39630)
                     /locus_tag="LK889_000616"
     CDS             complement(39358..39630)
                     /locus_tag="LK889_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094874.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(39713..39964)
                     /locus_tag="LK889_000617"
     CDS             complement(39713..39964)
                     /locus_tag="LK889_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094875.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40960..41871)
                     /locus_tag="LK889_000618"
     CDS             complement(40960..41871)
                     /locus_tag="LK889_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094876.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(42067..43902)
                     /gene="lepA"
                     /locus_tag="LK889_000619"
     CDS             complement(42067..43902)
                     /gene="lepA"
                     /locus_tag="LK889_000619"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK5094877.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            44049..45269
                     /locus_tag="LK889_000620"
     CDS             44049..45269
                     /locus_tag="LK889_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094878.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            45289..45783
                     /locus_tag="LK889_000621"
     CDS             45289..45783
                     /locus_tag="LK889_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK5094879.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            45761..46447
                     /locus_tag="LK889_000622"
     CDS             45761..46447
                     /locus_tag="LK889_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094880.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(46598..49207)
                     /gene="clpB"
                     /locus_tag="LK889_000623"
     CDS             complement(46598..49207)
                     /gene="clpB"
                     /locus_tag="LK889_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBK5094881.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(49458..50504)
                     /locus_tag="LK889_000624"
     CDS             complement(49458..50504)
                     /locus_tag="LK889_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBK5094882.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(50515..50787)
                     /locus_tag="LK889_000625"
     CDS             complement(50515..50787)
                     /locus_tag="LK889_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBK5094883.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(50822..51730)
                     /locus_tag="LK889_000626"
     CDS             complement(50822..51730)
                     /locus_tag="LK889_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBK5094884.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(51862..53094)
                     /gene="pepT"
                     /locus_tag="LK889_000627"
     CDS             complement(51862..53094)
                     /gene="pepT"
                     /locus_tag="LK889_000627"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK5094885.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(53109..54230)
                     /locus_tag="LK889_000628"
     CDS             complement(53109..54230)
                     /locus_tag="LK889_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBK5094886.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(54227..54928)
                     /locus_tag="LK889_000629"
     CDS             complement(54227..54928)
                     /locus_tag="LK889_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBK5094887.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(55072..55512)
                     /locus_tag="LK889_000630"
     CDS             complement(55072..55512)
                     /locus_tag="LK889_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK5094888.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(55641..56567)
                     /gene="dnaI"
                     /locus_tag="LK889_000631"
     CDS             complement(55641..56567)
                     /gene="dnaI"
                     /locus_tag="LK889_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBK5094889.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(56567..57940)
                     /locus_tag="LK889_000632"
     CDS             complement(56567..57940)
                     /locus_tag="LK889_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK5094890.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(57963..58454)
                     /gene="nrdR"
                     /locus_tag="LK889_000633"
     CDS             complement(57963..58454)
                     /gene="nrdR"
                     /locus_tag="LK889_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBK5094891.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(58766..59098)
                     /locus_tag="LK889_000634"
     CDS             complement(58766..59098)
                     /locus_tag="LK889_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK5094892.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(59150..59779)
                     /locus_tag="LK889_000635"
     CDS             complement(59150..59779)
                     /locus_tag="LK889_000635"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK5094893.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            complement(59743..60579)
                     /gene="mutM"
                     /locus_tag="LK889_000636"
     CDS             complement(59743..60579)
                     /gene="mutM"
                     /locus_tag="LK889_000636"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBK5094894.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(60624..63269)
                     /gene="polA"
                     /locus_tag="LK889_000637"
     CDS             complement(60624..63269)
                     /gene="polA"
                     /locus_tag="LK889_000637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK5094895.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(63395..64078)
                     /locus_tag="LK889_000638"
     CDS             complement(63395..64078)
                     /locus_tag="LK889_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK5094896.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(64071..64556)
                     /locus_tag="LK889_000639"
     CDS             complement(64071..64556)
                     /locus_tag="LK889_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBK5094897.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(64863..66197)
                     /locus_tag="LK889_000640"
     CDS             complement(64863..66197)
                     /locus_tag="LK889_000640"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK5094898.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(66328..67089)
                     /locus_tag="LK889_000641"
     CDS             complement(66328..67089)
                     /locus_tag="LK889_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK5094899.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(67105..67587)
                     /locus_tag="LK889_000642"
     CDS             complement(67105..67587)
                     /locus_tag="LK889_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBK5094900.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(67607..69787)
                     /locus_tag="LK889_000643"
     CDS             complement(67607..69787)
                     /locus_tag="LK889_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK5094901.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(69931..70662)
                     /locus_tag="LK889_000644"
     CDS             complement(69931..70662)
                     /locus_tag="LK889_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBK5094902.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(70731..71672)
                     /locus_tag="LK889_000645"
     CDS             complement(70731..71672)
                     /locus_tag="LK889_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK5094903.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(71852..72220)
                     /locus_tag="LK889_000646"
     CDS             complement(71852..72220)
                     /locus_tag="LK889_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK5094904.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(72831..74222)
                     /locus_tag="LK889_000647"
     CDS             complement(72831..74222)
                     /locus_tag="LK889_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBK5094905.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(74435..74821)
                     /locus_tag="LK889_000648"
     CDS             complement(74435..74821)
                     /locus_tag="LK889_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBK5094906.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(74877..75500)
                     /locus_tag="LK889_000649"
     CDS             complement(74877..75500)
                     /locus_tag="LK889_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBK5094907.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(75564..76232)
                     /locus_tag="LK889_000650"
     CDS             complement(75564..76232)
                     /locus_tag="LK889_000650"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK5094908.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(76244..76684)
                     /locus_tag="LK889_000651"
     CDS             complement(76244..76684)
                     /locus_tag="LK889_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094909.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(76743..77414)
                     /locus_tag="LK889_000652"
     CDS             complement(76743..77414)
                     /locus_tag="LK889_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5094910.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(77600..>78259)
                     /locus_tag="LK889_000653"
     CDS             complement(77600..>78259)
                     /locus_tag="LK889_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5094911.1"
                     /translation="YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFT
                     SKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNK
                     LSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKV
                     DKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT        22884 a        16419 c        12237 g        26719 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
    16561 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
    16621 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
    16681 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
    16741 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
    16801 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
    16861 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
    16921 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
    16981 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
    17041 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
    17101 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
    17161 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
    17221 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
    17281 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
    17341 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
    17401 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
    17461 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
    17521 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
    17581 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
    17641 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
    17701 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
    17761 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
    17821 ctccataaaa aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc
    17881 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa
    17941 atgatcccat atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18001 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18061 atcttggatt tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata
    18121 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18181 ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18241 aaccagtaat tacactatct acaacgccat tccaatttgc tctacatttt ccatttttca
    18301 gatttgtcgg tccatacgga tagccttttt gacattcaat gttcggatta taccagatag
    18361 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18421 atccaataca catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa
    18481 aatagaatag ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt
    18541 tctgtcctat aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt
    18601 ctgttttttt ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata
    18661 cgcactccgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt
    18721 caattgaagt aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata
    18781 ttgatccgga ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg
    18841 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct
    18901 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    18961 taagaaattt tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga
    19021 accaatttga ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc
    19081 gattgtttct tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca
    19141 accagtaaga cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt
    19201 tctagtcact gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg
    19261 tacgtgcgtt gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag
    19321 aaatgttgtc cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga
    19381 gacaatgcac cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt
    19441 gggttcatga ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc
    19501 gcaaacatgt gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc
    19561 gtaccaccat aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc
    19621 gcattgcctt tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa
    19681 tgcttgatcg caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct
    19741 cctcgataat acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga
    19801 atcaataaac aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc
    19861 gccaccataa agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa
    19921 ccaccttcag aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac
    19981 caggtaatcc caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat
    20041 tcatagccta attgagaaag aatcacactc ggtaataact gatactttac agctgcacca
    20101 actgctgctt gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc
    20161 ggtaatcctt cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca
    20221 tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa
    20281 agaagacaac ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt
    20341 ttggactgtt cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg
    20401 cagtgtttta ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga
    20461 aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg
    20521 ccccggttgt tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga
    20581 gtattggttc aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac
    20641 ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc
    20701 agaaggcaac ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct
    20761 ttgattattc ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct
    20821 tactgaattc atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt
    20881 tttcgatttg gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact
    20941 ataaggcaag gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc
    21001 ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc
    21061 agaagcgagt ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc
    21121 caaattttgt ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg
    21181 acttgctatg tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt
    21241 taataaatct ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt
    21301 tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa
    21361 aaatgccacc aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga
    21421 ctttaaggta ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa
    21481 aggaatcaca agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat
    21541 cactacaagt ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc
    21601 tttataaaca gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc
    21661 ttctcctgca gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc
    21721 cttggctaga aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt
    21781 cccataattt aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac
    21841 tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc
    21901 caattcatct agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc
    21961 atttaaccct ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca
    22021 aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt
    22081 ccctctaaat atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc
    22141 aataggatcg tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa
    22201 ctcgttcatt gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat
    22261 caatttactt aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc
    22321 cccaaataac attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc
    22381 aatatgaaat ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac
    22441 caatccacca acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt
    22501 cttttcctta tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt
    22561 atcctgttct tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat
    22621 ttcttcaaac aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca
    22681 acactgtcct ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg
    22741 taaatgattc aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa
    22801 attccctttt tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg
    22861 attcatctgg gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga
    22921 attattaaat acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt
    22981 tccaaacatt tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc
    23041 atctgtatat agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa
    23101 aatcgttgtt ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg
    23161 taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg
    23221 ttccatttgt tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt
    23281 ttgaactaaa acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc
    23341 taacggactt aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa
    23401 acaaacaatt ggatctaata caccataatt acggggattt gtagcatcta acgtgacata
    23461 actcattttt tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg
    23521 aatgagtttc gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac
    23581 attcataaac gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt
    23641 ttttccagtt actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat
    23701 ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt
    23761 tttttctcct cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga
    23821 ttccatacct aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt
    23881 tcggtgcttc caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa
    23941 ttctcgttga tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac
    24001 cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat
    24061 ccaatcgacg acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg
    24121 aacagttaat aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt
    24181 tttgaaccgt tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg
    24241 tggatagtga attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa
    24301 tagatcacaa tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact
    24361 actccctaac tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata
    24421 cgttccttga ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat
    24481 tcctcgaata tattgcagac gattgagata caacagctct ttttttacta agggacgaat
    24541 agaaaagcta taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa
    24601 agtttccaaa tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa
    24661 ccagattaga tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat
    24721 tcctaaaata aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc
    24781 ttttgcataa tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc
    24841 aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc
    24901 tgctaaaagc aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt
    24961 gttttttgaa attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt
    25021 taacaaacaa tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca
    25081 cctcccaata aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac
    25141 atacttattc acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata
    25201 atacacacaa tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa
    25261 atgcactttt tttccatcag gttttagttt tgccagtgct aacacacata aataggggag
    25321 tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga
    25381 acgtaaaaat aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc
    25441 aactggaaat ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata
    25501 aacaatcgaa taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa
    25561 tttttcaatg attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata
    25621 aactgcggca cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt
    25681 tcgcaatccc agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa
    25741 atctacaatt cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt
    25801 gttaattttt caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct
    25861 ttttgtaaaa gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt
    25921 tttttcgttt ctttataacg caatactttc atctctactt gatcaacttt agctgtttga
    25981 ttgagtcctt caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac
    26041 ttatctaaga aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt
    26101 tcgtataatg tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc
    26161 gtaaaataag gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg
    26221 agtacttgtg tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata
    26281 tcataagtta ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac
    26341 gaagcagcag ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt
    26401 gcataaaact ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat
    26461 gtattcatat acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt
    26521 aattctgacc gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac
    26581 gacacttgaa ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga
    26641 aataaaacca aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc
    26701 ggtactttct ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac
    26761 tccttctagt atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa
    26821 aaatagtcca ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca
    26881 aatccagcaa tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca
    26941 ccagtctttg gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt
    27001 ggaagtttct ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt
    27061 aatggaatac catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg
    27121 gttggtgttg gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt
    27181 ccttgagaag tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt
    27241 gctggaacaa taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc
    27301 ggttcttctt tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa
    27361 tcagtattct tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt
    27421 gaatccgttg ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt
    27481 gattcaggaa actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat
    27541 tgttcaggtt ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt
    27601 aatccttccc ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct
    27661 gcataaacca atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg
    27721 actgctaatg ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg
    27781 ttcatagacg ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct
    27841 aacccaacat atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt
    27901 ggatagtttg caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat
    27961 tcataacgat accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc
    28021 catcgttcca ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat
    28081 accaccaaaa ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat
    28141 catcgaacat tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa
    28201 acgttgttgc aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg
    28261 atcttctttt tcatagcatc ttgtagctct atgacgctca gcttgcaaga cataaccaaa
    28321 ctctgcacca tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca
    28381 atcacaaaaa tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt
    28441 agaaagcaca ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt
    28501 ttcatctgtg aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc
    28561 ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa
    28621 tccatataat gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac
    28681 ataccatctt ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt
    28741 tctttccttt tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat
    28801 caatcggcac aagttcacga aaacttggag caattttgga tagaatcact tccccatctt
    28861 gtaaaataca atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga
    28921 taaaatagta gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat
    28981 catcatagaa ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt
    29041 ctggtaacaa tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat
    29101 ctaaacgtct gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca
    29161 gatagaataa ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat
    29221 cacgtgacca atacgcacca acataaatca taattttttt catttagaca atcacaccag
    29281 caatactaat gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta
    29341 attctatctc aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca
    29401 tttcccaagt gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg
    29461 ctgccacaca gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg
    29521 caggtattgg caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc
    29581 taaattccaa ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa
    29641 tttcttgcca gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag
    29701 gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag
    29761 cctttaaatc caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc
    29821 tcctgacgtt cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc
    29881 aatcccttct tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa
    29941 tgtcggagct acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg
    30001 ctttactgta aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac
    30061 aatccgtgca tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac
    30121 gccaatctct cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt
    30181 tgctttctca ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc
    30241 ttctaattct gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa
    30301 aaagagagga ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc
    30361 atcatctaaa aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc
    30421 tgctttttgt ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca
    30481 aagttcatct aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa
    30541 ctttccttga tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg
    30601 catgacttcc tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt
    30661 tacagttcgt tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa
    30721 aagatacaag cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg
    30781 aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg
    30841 aagatctatt ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc
    30901 ctctgggatt tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat
    30961 caattgcgca aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc
    31021 acttaacttc tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata
    31081 aaataacaaa atcgattttt tttaaccctt tagaaacacc gatatatcaa ctagtccccc
    31141 tcgttttttg ggaaaagtag tacaactgct gaccttagat aatcacttga tacaaaaggg
    31201 tttataaaac ttttttctat gtgttatgtt atttctgccc cccatattat atatcggggg
    31261 gctttacaca aagaggccgt gcgcaagcgc acgacctctt tgtgagctgc acacgctgct
    31321 agcgagtgcg cattataggc ccatgcttcg cagccttaat gcgcaccgcc aggcagagtg
    31381 cggctaccag actacgtcta gagccctccc gatagataat aactacaaaa ttaaatgatc
    31441 taattttgtt atttcactat atttttcagg agaaaaaacg cgataaattg acaaagttaa
    31501 atcctcttct aaatctactc gataatgata caatcgagtc aatcgttcta tatctttttt
    31561 cgttagcaat acttgattaa taacttttcc tgtaaaaaca cgttctttgc gcaaaaagcg
    31621 gtaatcagtt acttgttcaa tacgattcaa ccaatcataa aacgttctgg aagaaagaga
    31681 aggattaatc attcgtattg ctgtagctac tctataataa caactctctg ccatttaact
    31741 tcacctcctt agttgattac aaaatatgcg catcagaaaa acttgttcct tttggatctt
    31801 ttgctaaata catttggcgc tctagttgtt cttgtaacaa ctgattatca tctcccatcg
    31861 tttgatcttg actatcctct tttactgtaa aatcatagac aatcatttca gtaaatttct
    31921 caaattcttt gataaagtga taatctttgt aaacaaatgg cgcaaaaaac tctacaggta
    31981 catcaaatcc tggaaaagca tatccacgac caggttgatc tttgttgtaa aatggcttat
    32041 ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg agacaatcga ccaacagaaa
    32101 tacggaatgt taaattatcc cgaacaccac ttggaaaata ttctgcatct ggtcgttgca
    32161 ttgctaaaat aatgtacatc cctgcttgac gaccctttaa tactaatggc tgaatcaatt
    32221 tatcgactct atttctcgct tccatatcca acgaaccata aaaagctgcc cattcatcaa
    32281 aaaagataaa atgcggtggt aacccataat aagcataatt tttccctggt tcatatttag
    32341 gatctttcat ggttttcata taggcatacc gttgattcat ttcttttaat gcatttcgta
    32401 aacaaataat catttcttca tttattttgt aaaacacatg tcctttgaat acatcgacgt
    32461 cacctaatgc tgctaaatct gccgcttttc catcgcaaat atctactgtc ccaacactta
    32521 aacaagcttg aattaaagaa tacaataaat aggttttccc accaccgatt gcaccagcaa
    32581 tcaacatatt tggtgattga tcatataccc aatccaaagt tttcattaaa tgaatacgtc
    32641 cattttctgc tatacattga tcaataggaa tccgaccaga atctgaaagg aatacataat
    32701 tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg caaaaatact tcagataaca
    32761 aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc atctaaagga aaagaaacca
    32821 ctaattcttg tttcttttga taaaaataaa cttttggtaa tctcattttt tcccttgttt
    32881 gtttctcact aacttgtttc tttgtataaa caaaccgatt gtcaattaac attcgaacaa
    32941 gtaactgacg ccaataaaca gaggcaaaat atccttcttt tagtcgcgac caatgcatca
    33001 accctaaaat taacccacta accagcaaca aatacaacac aatacttatt ccaggaataa
    33061 gatacatttg ccaattcgaa aacgtactta aacgtggata aaatactttc caactaaacc
    33121 aacctaacag aatcaaaaat ggaaacaaaa agaaaccata atagcaaaac cataaataac
    33181 gattgaaata gcgaattcgc cgaccacgat acttgaaaaa atgagctttc aattgtcaca
    33241 ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt ttgacaaaaa gcaactaata
    33301 caggaatatc acatttagta aaaaaacata atcgaaaaag atttaatcga tttttataag
    33361 ggtgtgcaca catattacat attcgtacta tacgaatcca aatcacttcc tgatgatctt
    33421 gtttccactg gtacggtaat gaaaaacgaa caagatcacg taaaaacata cactgcccaa
    33481 taaccgcaca aatatatgca tttgtttgta caaaaatcgt tggattgtct ccaaaatact
    33541 caatcaattg ttggatattt tgataacaag cacaatctct caaagtattt gattggtttt
    33601 atctgaattc aaaatctccc cttccttcta tcttagataa aaagaatagg aagactcctt
    33661 cctattcttt caaaaaaacc ttattttttc tctttttcct tagaatcaac acttggttgt
    33721 ttttgatcta aacctttttc ttgtgattgt ttcatattag gattatttgt tggagcttct
    33781 actttctcta atcctttcgc ataaatcact aaatctgaat attcacgatt gccatcttgt
    33841 gctggaatat ataccacatg atattctgga tcaattaatt tcacaggatc acgtcgttta
    33901 aaaccagtca aatctacatc tgctggaaat ttaactcgtc gtgcaatctt tggttcatct
    33961 ggatttccaa aaactaattg tgctcgctgt gatcgaattt ctcccgtata atcgcctgtt
    34021 tcattatcaa atacacgatc agactcttct aatgcgccaa agcgtacggc tcctaaacaa
    34081 gtttcatcta atgcttctgt aatgtcataa ttatttggaa attttaatcc catcttacaa
    34141 aacctcccca atttttacac cagttaaaaa gacaccaaca tcttgatagc cagttgttcg
    34201 acgctctgtt tgtcccatat catttgtttc acgactattc actcgttctt tacgaaaaac
    34261 aaaataagct tcttcaaatg aaatacgcac ctctttttct ggtaatcgta atcttttatc
    34321 ctcttcagct gtgagataaa caaaaaaagc aggctgttct tctcgcgaac actctgcttg
    34381 taattgaaca tatcgcccag acacaccatt aatttccttt ggtggattag gttcccgtga
    34441 acccaataaa aatacttgac ctaaaaactc tttcttcata cgcaatgctt tttctgccat
    34501 aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa tatttctaca ttacaatcaa
    34561 acaagcggtt aaaaatccta accgcttgtt cctcatgact aatctttggc tttacgtact
    34621 ttataaagtg ttatacctaa agcaatgact aatactacta atccaagaac cataagtata
    34681 ctacttgttt gttcacctgt ttttggcaac attggtacaa cagtagctgg tttaacccgt
    34741 tgttctggtt tcactggttg cggtggtttt ggcgcttcca caatttgaac cgtttgatct
    34801 ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa acgtttcttt ctcatcttcc
    34861 gttttcgctt ggagccattc cgcaaaactc aagccttttc ctgcaagatt gcgaccgtcc
    34921 actaagaatt caaataagaa ttccatcatt tctttatcgg ccgcaaagac tttttccgca
    34981 tgagtgcctt caaatggtgt gccgtctggt gttaagtagc cttgaacaat taagcgatag
    35041 atttgaccaa tcgcaagacc gtctactttg acccattcaa gcacagtttg atttggcaag
    35101 gccaagatct cttttccgcc atttttattc gtcgctttcg tcgtaatggt tggattactg
    35161 attttgatgg tttggtcatt gtcggtaata tctgcgtgag aagccaataa tacgccttct
    35221 ctaaacagat cttcaaagac cacgacttct ttgcctcgca acgcacgtgc gtctagaata
    35281 aagtctagat tgactgtacc atccgttgtt tctgccacaa acgttaaggt acttgtgact
    35341 tcttttccat ctactagcac tggtttttcc gttactttgt ccattagaac accgttgact
    35401 gtgtatgttt ttccaacgac caaatcgtgg tatttcacag cgtctgtaat ggtgactttc
    35461 gttaatggat taacctcttt caagccagtg ggtttatgtg tagccgttgt acgtacactt
    35521 gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt ctttatggtt tgctagttct
    35581 tcatcatttc taaaaagact ttcaaacacg acgatttcac gaccatttat acctgtcaaa
    35641 tcaaaggtaa atggtacgat caccgtttca ttttctgctt tggcaataaa gccaacactc
    35701 gtttctaccg tatttccatg cacaagcaag ggttcaccgg ttgctttatc cattaagatc
    35761 cctttgacca agtatgcttt tcctggaaga atctcggtaa tttcaacggt atcgtttaag
    35821 gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt caaagacagc cgtggtatga
    35881 acggttggat tattcacttt cacactttgg gcttcatcgt tgatatccgc atgtgctgca
    35941 acttcaatgc catcacggaa aagtttttca aaaacaacga tcgtttgacc tttcagaaca
    36001 ctagcattaa ccacaaattc aaggtctttt gtgccattga ctttgtctgc aataaaggcc
    36061 aaggtacttg tgatttcttt gccatcttgt aaaattggcg cattcgttgc cttgttcatc
    36121 aatactcctg aaatggtgta ttcttttccg acaaacaagt tggtataaga aacactgtct
    36181 ttaatgacga tcgtttctaa tggatcaacc actttttcac catcggcttt atttgacgct
    36241 tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag aaaccactaa cgtttcccca
    36301 tcatctggcg cattttgttt gaatgcaacc gtgaatgctt gtttatctgt atttggttgg
    36361 aaactttcta atggggcatt ttccaagaag taatagtcat cggcaactag acctttcact
    36421 aaaatttccc cgttttgatc agtggtatat tcaccaattg cttgattttt accgttatac
    36481 aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt tccattcttc atacttgaat
    36541 aattttacac ttttccgtgc ttgacggttc gcaaaatgga tcactttgat cggttctgta
    36601 tcattggtaa tcgagaacac taatgggttt tgattcaaaa cgtaatttac gggggctttg
    36661 atttcttcca aacgatagcg atcttttcca taagtaagtg taccgttagt taggaaaaag
    36721 cctttttcat tggttttaaa gatatccgtt gtttctgtat catttggaat ctccattgtg
    36781 acataacgat tggctaaagt atcaaaaatc ttgaattctg tattagcaag tggaatcact
    36841 tttcctgttt cttgatccac tttcaccact ttaagacgtt gttcaattac tttatcttct
    36901 aaaacataat ggaaagtttg cccattttca gaaattgtga cttcaagtgg ttcaaatagt
    36961 ttataccctt cttttccttt ggtttcagaa agaatgtacg tatcaaagac aacggtattt
    37021 ttaccaaaag tagcataacc atcattgtct gtcacaacgg ttttcaccac tttgccagtt
    37081 gtttttgacg tcaaagtgat ttcaatgcca gctaattttg gtttgttatc tggattcgtt
    37141 gaatcgacta aatctttatt agcaactttc actaaatcaa tggttccttt gatcacttga
    37201 tcttttgcgg tcgcattgac agtttttcca ggttcaacaa caatttcaat tgcttcatca
    37261 gaaagcacat aaccttttgg cgctttgatt tcttttaaaa catatttgcc aggatcaaga
    37321 tctttaattt ctgcctgtac tttgccgtta atttttttca aggtaatttc tttaatcttt
    37381 ttcccatctg cttgatacaa accatagacc gcattttcta aagaagctgc accttgtgct
    37441 ttcacaccag tttccgcatc ttctttaaac acacgaatgt ttccttgttt tagaatttgt
    37501 aaattaataa cagacgcatt aggttctgtt ggatgtaaca caccaattgt ttgagctcct
    37561 ggacgacgat aggctaatgg cgcaccttga actttaattt tattcaattg aatttttgct
    37621 ttatccggtg cctcttttgt gactgtaatc catacttcat tgccgtgaat agatacattg
    37681 acaccttttg gtttttgaat gtctttcaca tatttcaaaa aatcattttt attctttact
    37741 ttcaacgttt cgccaatttt taccgtatgt gtttgattat taaaagttgc tttttcacca
    37801 aagttattaa tcaaacgttg aatttctgct tttcgacgat cataatcaac acggatattt
    37861 ttgattggga tttttgtgcc caaagcttcc caaatcataa attgtgccgc aatgtattgt
    37921 tcatcggatt tgtctttatt tgtgacatat cctaaataag agatcaagct tgtttttttc
    37981 cgtacatttg aatttttcaa atagtttttc aaagcagtag ctgtataaac ttgaccttct
    38041 gcggctgaga caccaggctc aatacaaaac gccacttgtc cattaatctc aaaacgaacc
    38101 atataattta aagtgaagtt tagattgttc ggactagaag taacaccaat attttcgccc
    38161 caatcaattt tccgaccacc taaaacagct gcattggttg cacggcgaac acgaaccgaa
    38221 cgattggttg gtactgttac taacagtgta ggtgtacctg tttttggaat gttctgctct
    38281 tttttctttt gattttcaat actagccgta gattctttgt ttggtttcgt tggttgattt
    38341 tgactagctg cggttgaaga gattgttttt tcaacgtttt cctcattttt tccttctttg
    38401 tcagcagtag ccgctggttg ttccactgtt tcttctattc gtttcttttc ttccgcttgt
    38461 ttctcagaag actctggctg cacagaaggc gttgcttcgg cagctttttc tccttctaga
    38521 gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt cttcaactgg ttgtgaagta
    38581 ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct gatcgagagt cgttttggaa
    38641 ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag tggcttcacc agctgggacc
    38701 ccggcttctg ctggttggac ttctgtcact tcagaagcga cttgggcaat ggcttccatc
    38761 gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag cagttcccac caagaaacag
    38821 aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat tcattttcgt ttttgcatac
    38881 acaagaattc ctccttttcc tgagaatttt atataataaa acaaaacgcc gttaccaaaa
    38941 gtgataacga cgtgaactgt ctcttctatt tttatctggc gctcttatct gaacacattt
    39001 catcaatgat tttaccctcc cactataatt acagaacaaa aaaagaacct gcttcgtgga
    39061 aatcaactgt ccatttccca attggaatac cagctttttt ggcaacttca ccatcaccaa
    39121 atctcatgcc ataccaatta ctaccttcat ccttaatttc tttccaacca tatgacatag
    39181 cttcatctaa tgaatcaaaa atcatgacac tattgccaat tgttacagga tctggcttaa
    39241 gatcttctgg aagagaaata tcaatcatac cataatgatc cgattcctca gtattattgt
    39301 cattactagc tactggtttg gttgttctac tggcttattt tgatacaaca agtactctta
    39361 ttcttctttt acttctccgt tttcaatttc ccattcaaac cactccgcaa agtcttgctt
    39421 aaaaccatct actcgatacc tattttgtaa ataggcatct tctgacgtcg taggatctaa
    39481 ataggtaggt ttattctctg gacgagcata gacacctacc catgtttcgt aatctccttc
    39541 aaaataacta tctaccaaat agcctttttt tctaagaatc tcccgaactt gatttttaat
    39601 tattgatact tgttcaaaac catcactcat tctatcacct caaataactc tagattctca
    39661 taaatattcc cgacaatttc aatcctatca atgatctcta acaaatcgct acttatgctt
    39721 ccccattcgt ataagaattt cccttccgca aaataaattt tccataaact tcttcatgat
    39781 cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt cttatctttt agctcagtaa
    39841 atgccataag ttcaacatgt tttccaacct cgaaacaatc acaccaccca tcaaccgtta
    39901 cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt attaaacact aaaactttcc
    39961 gcattttttg ctcattaatt aatcacgcgc gaaatctagg aatcacaact tattcgcttc
    40021 ttctttataa ataaaaaaac tccaataatc ggaaattact atcttttcct cattcctttt
    40081 ttaaatcttc atacttcttc ttatctttgt cctaacctaa taacacaaat tttgaagaaa
    40141 aactcagtaa aacctgattc tttttttatc ttcacaacaa ccaacttttt cttcagatcc
    40201 ttttactaga acttgtatac aaaaattgtt gaatgctgat ataatttgcg gctacttgaa
    40261 aaaacgatag atgtaaaaag ataaatagaa tcatttttta ttctatatca gtctatgatt
    40321 cattgcttat caatcaccca acccattcga tgccgttttt tttagagaaa tttaaaaaag
    40381 accgtacgaa tctcgcatta tccgattact tttccagaaa agatccccaa ttctgtataa
    40441 ataaccctat cttcttgttg gtttcatttt ctcatataag aattcagtca atctacccac
    40501 tatgaccatt actacgtcca acacaactga catattaacc acgtaagtat tccctcacag
    40561 atctccgaat ctccatggta tggattccca tcctgacgct gcagaggaca acatatttca
    40621 tgtggctctg tacccttttg atttggaaac actacaaatc aatactcatc tataacttat
    40681 caagcgaact ttcgctaggc ttctagtgat tactaaattg ttggataaca tacatagccc
    40741 attttatcta gctgttattc ataacagtta gttcttgtat tacttcttcc gcttgtgttg
    40801 ccattgagct tgtcaaaaag ttacttagaa aaaagctgcc agtaaagcaa ctttcaacaa
    40861 accctttttg tttcttatca tatcaagaaa cctcctttta taaatttttt ctataataaa
    40921 aaacgtcgat aacttaataa gtatcgacgt aaactatttt tatatcccgt aaatcaaaaa
    40981 ttctaaagaa agatttccta tataagcata ttggataatt cgattagtat tgggtatatc
    41041 tctaccattc tcatacctat ctatggacca ttttgaaatt gaaggagaaa aaagctttcc
    41101 aaactcctcc ctttctagat aataatcttt cctaattttc ctcatcctta atcccatact
    41161 aaaagaattt atctttttaa aatcgctaac tctttcatct tttggaagta tattcttata
    41221 atcttctccg tacataaggt aaggaacagt gaccttacca agataggcaa tattcattag
    41281 tttttcaata tctggaatat taataccttt ttcccatcta cttataacat ttttgcctgt
    41341 atagatggta aagtttttac caaatttttc aagcgtctct tcccggttct ttcggacttc
    41401 tcgtattctt tttcccataa caattttatc gtcttttgat aaactaaata tctcactatc
    41461 acgattaatc tccttcaaaa tttctttttc attacttaat agaaaaatat cgattttttt
    41521 ttgcatttct tttgacggat ttctctttct atttaacaca taactgagta atacttcact
    41581 aattcctata ttatcagcta actctttgtt aagtaactta ttcatatctc tgtatgcaat
    41641 tagtttaccg atgagaatct caatcttatc ttcctcatct tttattagag tttctatatt
    41701 cagaatcttg cttagagtat tatctacgtc tttagggatt tttcttgacc ctttttcata
    41761 tttaactaaa gtactttgtg aaatacctgc taaatcagct aacttttttt gagatagttt
    41821 tttctctttt cttagagatt taagaaccct tccacttgaa ttatcaacca tgaaaaaagc
    41881 acctcctatg agatgctttc atcatatact gacagctaaa aatcttcaag aaaaatattc
    41941 caattttgtt aattttagta tcgccgtgat aaccaaatga cagtttaaaa tcttttatac
    42001 agataatcct cagtgttgca tctactcaaa aaggtttatt taataggttt ttcgattgat
    42061 tataagttat ttcacactat cttgctcatc cattttcaga acggccatga aggcttcttg
    42121 cggaacttcg acggagccga tttgtttcat ccgcttcttc ccttcttttt gtttctctaa
    42181 gagtttacgt ttacgagaaa catctccacc ataacattta gccaagacgt ttttacgcaa
    42241 ggctttgatg tctgaacggg caacgatttt ttgtccaata gctgcttgga ttggcacctc
    42301 aaattgttgg cgtgggatta gttttttcag tttttcaacg attgcttttc cacgttcata
    42361 ggcaaagtct cggtgaacga taaagcttaa tgcatcgact ttttctccat tcagcaagat
    42421 gtccatcttc actaacttgc ttttttgata accagacatt tcataatcca atgaggcata
    42481 gcctttcgtg cttgatttca atttgtcgaa aaagtcaaag acaatttcag aaagcggaat
    42541 attatagacg acatttactc ggtaatcatc taaataatcc atcgtaatga attctccccg
    42601 ttttcgttga gaaagttcca ttactgcacc gacaaaatca tttgggacca tgatctgtgc
    42661 tttgacgaat ggttcttcta catcctgaat cgttacaggt tcaggaaaat ctgccgggtt
    42721 atctactgta gcagtcgtac catccgtttt attaacatgg taaataacgg atggtgcagt
    42781 cgtgatcaat tctagattga attcccgttc caagcgttct tggacgacat ccatatgaag
    42841 aagtccgagg aatccacagc ggaacccaaa acctaatgct tgagaggttt ctggttcgaa
    42901 ttgtaaagca gcatcattca attgcagttt ttctaaagct tcccgcaaat cgttgtaacg
    42961 agaagtatca attggataaa gaccacaata aaccatcgga ttcatttttc ggtaacctgg
    43021 taaagcttca gcggcaggat tgtctgctaa cgttactgta tcccctactc gtgtatcttg
    43081 gacagttttg atacttgctg tgatataacc gacatcaccg accatcaaaa agtctctagc
    43141 aactgctttt ggcgagaaga ccccgacctc tgtaacatca aatgttttgc cattactcat
    43201 cagtttgatt ttgtctccgg gtttgaccat accgtccgtg atccggacat ttagaacaac
    43261 accgcggtaa ctatcataaa tagagtcaaa gatcaaagct tttagcggtg catctaagtc
    43321 tccacttggt gcaggaacat attccacgat ctgctcgaga atatcttcaa tcccaatccc
    43381 tgatttggca ctggctaata cagcttcact agcatcgatt ccgattacgt cttcgatttc
    43441 ttgacggaca cgttcgggat ctgctgcggg taggtcgatt ttattgatga ctggcaggat
    43501 ctccaaatca ttgtctaacg ctaaataaac attggccaat gtttgtgctt cgattccttg
    43561 tgctgcatcg accactaaaa cggcaccctc acaggcagca aggcttcgag agacttcata
    43621 ggtgaagtcg acgtgccctg gggtgtcaat caagtgaaag atatatgttt ctccattttt
    43681 cgctgtgtaa ttgagttcta cagcatttaa tttaatcgtg attccacgtt cacgttccaa
    43741 atccatcgag tctaacagct gatcttgcat ttcgcgagac gtcacagtgt gcgtcatttc
    43801 taaaatacga tcggccaatg tagatttccc atggtcgata tgagcgatga tcgaaaaatt
    43861 acgaatctgc tcttggcgtt ctttcatttt ctttatatcc atagcgtctc ctcttttcat
    43921 ataatcagca ctttccatta taccaacgaa aagcagtaat ttaaagagtt tttaattagt
    43981 cagctataga agtttttttt gtagaatttt gctatactgt tatcaagcta ataaatggag
    44041 atgaaaaaat ggatcaaaaa gattttagag aaaatcttga attaaaaccg gtcactcaag
    44101 aacatatcga tcaattcaat gaattgcttt cctatgtgtt ccaagtgact gaagcagaca
    44161 tcgaggaaag cgggtttgag aataaacgag cttttatccg ttccaaaaaa ccgattttgg
    44221 aagtatcgaa agtgttcgga tggttccacg aaaataaact gatctctcaa atcgctatct
    44281 atccttgtga agtcaatatt cacggtgcac gttataaaat gggtggtgtg actggagttg
    44341 gaacttatcc agaatatgct aaccatggat tgatgcaaga tctaattatc gttgcattgg
    44401 ataatatgcg caagaataag caatggatct cttatttgta tccttacagt atcccctact
    44461 atcggcgtaa aggatgggaa atcatgtctg ataaactttc ctttaaaatc agagatacac
    44521 agctacctaa acaaatccca gtaagtggta tggttgagcg attaccagtt gatcatccag
    44581 acgtattttc cgtttatgac aaatttgccc aacaaaacca tggtgcttta ttccgcagtg
    44641 attttcattg ggaagagtat tggcgtttcg aaaatgaaga tgagcgcaca gccgctgtct
    44701 actacgatag caaccatgaa ccaagaggtg ttctgttcta ctgggtagct gaagaaatat
    44761 ttcacgtaaa agaaatgttc tatcttgatc aagaagctcg aaatggctta tggaacttta
    44821 tttccgcaca tttttccatg atctactggg tacatggaga tatttataaa aatgagcctt
    44881 tagcattttt gattgaagat agtcagatca aagagcagat cgaaccatat tttatggctc
    44941 ggatcgttga tgtaaaagaa ttccttcaac ggtttccgtt tgttggaaca gctgatgctt
    45001 ttcatttcat cattgaagat cctgtcgctc cttggaataa tggtgtcttc gctttaacat
    45061 gggatgaaca gggacaagtc cgtgtcttaa atgaacctat aggaaaatcc gttcgactgg
    45121 atatccaaac attgacttgc ttgatgatga attatcgccg tgcttcttat ttggcaagaa
    45181 tcgaacggtt agaaacagat gaagaaacac tgaagtcact agagcgtatc attccaaaca
    45241 tggaagctta ctttagcgat tatttttgat aaaaaggatg atgtttagat gaaaatttat
    45301 tttgccgcac caatgtttgc taaaagtgat ttgttatata accaacagtt agttaaagaa
    45361 atcagagagc tctcttctga gctttcgatc tatcttcctc aagaaaatga agcaatcaat
    45421 gacaaaactg cttatgcaga cagtcgaatg attgctcttg ctgacacaga aaaagtgttg
    45481 gctagtgatt taatgattgc tttactagat gggctgacga tcgatgccgg ggtagcatcg
    45541 gaaatcggtg tggcttatgc gaaaggcatc ccagtgatcg gcttatatac tgatactcga
    45601 cagcaaggcg gaacccaccc gaaaaaaatc gctgcactac aagaaacagc tgaaaatcaa
    45661 tttcactacc tgaacttata tacaataggt ttgatcaaat tgaacggaaa agtggtttct
    45721 tcagaaattg agttgctttc agaagtaaaa agatttttag atggagggac tttcagtgat
    45781 taaagaaatc aaaaaggtgc aaatagcttt acttgctttt ggtgtattcg ttgtctgcta
    45841 taatttttat gaatttatca cacaaaaata ttcgacttct caaggaatca catttatcgt
    45901 ggaatcttta ttagggattg cattgatttt catgccacaa gtcattttga aagttttcaa
    45961 acttaaaata ccagcagcaa tcgttttgtt ttactggttt ttcttgttca tctctgtctt
    46021 tttaggcaca gggatgcatt tgatcagtat catctctttt tgggacaaaa tcttacatgc
    46081 ggtgagtcca atggtcctga cagctcttgg ctatggattg attggttact tgatgaaaga
    46141 tgctgagata tcaaaaacca gtccttggtt gttcctctta ttcggctttg cgtttgctgg
    46201 actttgcggc gtgttttggg agttctggga attcttatgc gaccagttct taggcatgaa
    46261 tcttcaacgt tttgctgctt ctgatggcac actgtttgtc ggacgagcag cattgatgga
    46321 tacgatgggc gacttgctga caaatacgat aggtgctgcc ttaatgggat tgtttgcatg
    46381 gtctcaaagt aaaaaagatg agcgttactt tgaaagttat aaattagaaa aagtgaaaaa
    46441 tacctaataa aagaggttgt gacagaagcg ggcagcttca agaaatttcg gcttattttt
    46501 cgaagaaact acttctggaa cactgtttat ttgaaaaaag gaggtctgac actagttgtg
    46561 tcagacccct tttttctttt atagtctcaa tggaagatta gacgatttct tctagctctt
    46621 ctgttttgaa atgaagttgt ccatcaagta aagtaatcgt tacttttgat ttaggcataa
    46681 catgtcctgc aacgatttct ttagctagtg gtgtttctac ttccttagtg ataaatcgtt
    46741 tcaatggtct tgcaccatat gctggttcat aagcattttc agcaatccac gttttggctt
    46801 catcactgat tgtcagcagg atttcttgat gttccagacg ttgtgccaat tgcgccacca
    46861 ttttgtcaac gattcctttc acgttgtcta ggctcaatgg tgtaaataaa atcgtgtcgt
    46921 cgattcggtt caagaattct ggtttgaagt ttccacgtaa tagcgtgttg acttgttcag
    46981 ccactgcttc tggaatcgtt ccatcagcag tcacgccttc cagcagtaac tgtgaaccga
    47041 tattgctggt catgatcaga accgtatttt tgaaatctac tactcgccct tttgaatcag
    47101 tcaagcgtcc gtcatctagg acttgtaaca agatattgaa gacatcggga tgcgcttttt
    47161 cgatttcatc caacaagacg attgtataag gatttcgtct gacagcttcc gttaattgac
    47221 caccttcttc gtaacctaca tagcctggag gagctccgac tagacgagac acagcatgtt
    47281 tttccatata ttcactcata tcgatccgta ccatatgatc ttctgagtca aacaagttct
    47341 cagctaacgc tttcgctaat tcggtttttc cgacaccagt tggtcctagg aaaaggaaag
    47401 aacctagagg acgatttggg tcttgcaatc cagcccgtga acgaattacc gcgtcactga
    47461 ccgcatcaac tgcttcatct tgaccaatca ctcgtttatg aagtgtttca ttcaatttaa
    47521 ttagtttttc tcgttcacct tctacaagtt tcgttacggg gatacccgtt aatctgccaa
    47581 cgacttgagc aatctcattt tcggtaacgg actcttgaac catcttaatc tcgctgtctt
    47641 ttgctttagc ttctaattcc ttcaattctt tttctaattg aggtatcgta ccatgacgca
    47701 atacagcagc gcgttctaaa tcatagttat tttctgcatc ttctaattcg tgttttgctt
    47761 tatcgatttc agcacgtttt gctgaaacag aattgacttc ttctttttct gtttcccact
    47821 gcatcttcat cgcatttgct tcttcacgaa gttcagctaa ttcttcttgc aaatttttca
    47881 agcgtttttt acttgcatca tctgattctt ttttcagtgc agcttcttcg atttccaact
    47941 gcatcaaacg tctagtaact tgatctaatt ccgttggcat agagttcatt tctacccgaa
    48001 tcgttgcact ggcttcatcg atcaaatcga ttgctttgtc cggtaagaac cgatcagtaa
    48061 tataacggtc agataaagta gctgctgcta ctaatgcgtt gtcatggatg ttaacaccat
    48121 ggtggatctc aaaacgttct tttaatccac gcagaatact gatcgtatct tccactgttg
    48181 gttctttgac taataccttt tgaaagcgac gttccaacgc tttgtctttt tccatatatt
    48241 gacggtactc atctaaagtt gtggcaccga tcaaatgtaa ttcaccgcga gcaagcattg
    48301 gtttcaacag atttccggca tccatacttc cttctgtttt tcctgcccct acgatattgt
    48361 ggatttcatc gatgaacaag aggatacgtc catcactttt tttgacttct ttcaagactg
    48421 cttttagtct ttcttcaaat tcaccacgga attttgctcc agcgatcaat gcacccatat
    48481 ctaatgaaaa aatcgtttta tcttttaaat tttcaggcac atctttgcgt acgatacgtt
    48541 gagcaagccc ttcaacgatt gccgttttcc ctacaccggg ttctcctatc agaacaggat
    48601 tattttttgt ttttcgtgaa agaatacgga tgacatcacg gatttcttca tcacgaccga
    48661 tgattggatc catttttccg cttttgactt gctgtactaa atcaacgccg tatttttcta
    48721 atgctttgta ttgttcttct tggttttgtg aggtcactct ttctcctcct ctcatctctt
    48781 cgatgttttt acgcaattct ttttcagata atccgttttt attcaaataa acagttaacg
    48841 gataattttt taatttcatc aacgctaaaa taacgacttc cgttgaaaga aactcgtcgc
    48901 cgaagctttc acgtatttgg tctgcttcat tcaataatcg aaagaaattc tggctgaggt
    48961 tttgtccata ttggacgttt ccaccagaaa caactggata ctcatccagt agacgatcaa
    49021 tttcatgttc aaaagattcg acatccaatc cggcatccgt atagaaattt cgaccaaaat
    49081 gattagattg taaaaagatt ttccatacat gggcaatgtc gatatcttga tgttttcgag
    49141 tgaccgcaat ctgttgagct tcagcaatcg cttcttgaag cgtcgtagtc attttctcaa
    49201 tattcataca ggtataccct cctaattaaa ggtaagatca cttatttctt tgatctttac
    49261 aaaaactatt atatcgcttt ggtcaatttt ggtcaaaata tacacatagt tttttgactt
    49321 tttttctcga cttatataaa tcttaaaaaa acaaaattga ttgtatgtat ataaaaaaag
    49381 agccaaacaa acgtaaaatt atcttatcca taacgttttt ctttgatttg ataaaaaggg
    49441 tagatcttat aaaagaccta cccttgattt tctttattct ctttttttac ttctattatt
    49501 tcttctttga tcactcttcg ccgttcagca atcacataca caaatccata gagaaatggc
    49561 aagataaagg aactgaaacg atacagtaaa agcgaagaaa ctgcgtcaac tgttccaaca
    49621 actggtctga ataataacaa atagacaaat tcaaaggaac caataccagc aggcgtagga
    49681 ataacccctg aaagcactac tgcaaagctg ataaacgaaa tggtcagtag aaaatctatt
    49741 ttaggatggt tttcaatcag aatcaaataa ggaagtacat accaaaatac aagttttaac
    49801 atattccata gataaatccg caatagcgca gtcctgtctt ggataatggt ttggaccgtt
    49861 tcccttaaag agtagatctg ctgattgcat gtatccaccc atttccgcaa acgctcactt
    49921 ttaaataact tattcgataa gctgacaaaa aatatttgaa cattcaagct gacggacatc
    49981 gccaataaaa aggcaacaat cacgatcgtc aaaaggatac cagcgatgat aaaccagatc
    50041 attgatggac cattagaata aaaaagagag aattggatga ccaagccagc taatgcataa
    50101 gtgatcactg ccagtttgta cataatcata tggagtgctg tcacccctgc tccctgggat
    50161 agcttcattc cttttttgtt ataaaaataa atctccgaga tcagcgttcc tgtaccaaac
    50221 gaaacgattc ggtaaaacgc aatatagcta gaggtcaaga acccatcttt cgttgtaaaa
    50281 ttcggcgcga aaggttcagc aatttctttg attgagcgcc cttcagcgat ttggtaaact
    50341 gtccctaata taacagccgc tgccaaaaca acaacactgg tagacagcaa ttcggcaaag
    50401 atatctgcaa atgattgatt gattaaataa taaataatgg caaaaatcac aatcagcaaa
    50461 agcagattca gaatgatttt agttttcgtt cctttagacg acatctcctg ctccttaatg
    50521 gaaaaaataa atgagaatca aatcaacaag tacacctgca gcgaatccta tagtaaactt
    50581 ctttttccga gttttatgat ggaaaacttt tcgagagatc aatcctccca aaccaccgcc
    50641 taacaggctg acaaatatca aattattctc aggaacacgc cagccgctag ttctagcctg
    50701 atgtttatca taccccatca gacaaaataa gtaaaaattc acaatgaaaa ggtaaaacca
    50761 caaaggattg tgtaacaata catccattac tcttcttcca actttctgtt aactactatt
    50821 tctaagcttt ttcttctttc tttggtgaat aaactgttaa tccttttgat acaacagata
    50881 attcaactgg aagatcatct gtcgtgtctc catccgttcg agtgcccaca gatttttcct
    50941 gcgcttggat tttcatgcgt ttggctgtga aataagtaat gccggggatc gtatagaatt
    51001 tgcctctcat gattctaggc aaataaaaaa taaatcggaa aatatctaag gaaggtacga
    51061 tcgttacatg aaattttcca tcatctaggg tcgctgagtc gtcaaaattt gtaaaaccgc
    51121 ccacggagtt agacatcgtg actgtcaaca gttgggattt tcctttccaa tgctcctcgt
    51181 ctgtttctaa atccaaatga tattttttct tcttcagcaa taacttaaaa aacttcttga
    51241 taaagatcca cgagccgtaa cgctgttttt cttcttgact gatccaaact gctgtatctg
    51301 ctaagattcc gattgtcagt gtagagataa tgacttcatc attgacttga ccatagtcta
    51361 ttttccgtac attgccttcc aggatcacgt cagcagcttc ttcttttttg aggggaatct
    51421 caagaacacg tgcaaggttg ttgacagttc cacctggaag taatccgaca tggtattgag
    51481 ataatgtttt ttggaacgct tggacgatat gatgaatcgt gccgtctcca cctataatta
    51541 ctaaagtatc agcttctatg gcttctgctt tcttgactaa ttccgcatca tcaatcgatg
    51601 ggccagtttc agctagttct acatataatt ctggctgctt ttcttttaca taatcttgaa
    51661 accagcgtgc taatttctca ccctcatctt tccctgaact tttgttataa aaaactagca
    51721 gcttttccat tctttacctc ctatttactc ttcaaaaaag agggcgggac aaccaattcc
    51781 attgtcctgc cctttagcct tagacagatg acattccatc attgggacga acgccttgtc
    51841 ataagctgtt ctttttcgat tttaactctt tgtgttcaat tctgcaattt ttacaatcac
    51901 gtctgttgct ttttccatta cttgtaaaga aacaaattcg aatcgtccgt gcatattttc
    51961 tcctccggca aatagatttg gcgtcggaat acccatgtaa gagatttttg aaccatctgt
    52021 tcctccacga actggttcga tcactggaga gataccaagc tcgatcattg cttgtttcgc
    52081 taattccacg atgctcatat ctttttcaat gatttctttc atgttgtaat attgatcata
    52141 catatctact ttgattcgtt cttgatcaaa tcgctgattc aactcttctt ggatatgtgt
    52201 gatttgtgct ttgcgtgctt cgaatttttc acgatcatga tcgcgaatga tataactcat
    52261 agaagcttct tcaggattcc catccaatgc catcaaatgg aagaaacctt cgtatccttc
    52321 cgttttttct ggaacttcat cggctggaag ctgattatga aaatcgattg ctaactgtag
    52381 agcattgatc attgtatctt ttgccgtccc tggatgtaca tttttccctt ggatcgtaat
    52441 atttgcttga gcagcactaa acgtttcata ctgcaactca cctaaaggac caccatcgac
    52501 tgtataagca aaatctacat cgaattgctc aacatcgaat ttatccgctc cgacaccgat
    52561 ttcttcatca ggaccaaaag ccactttgat ggtaccgtga gggatagaag gattattgat
    52621 caagatctcc attgcagtca tgatttccgc aatccctgat ttgtcatctg ctcccagtaa
    52681 tgtactgcca tctgttgtga tcaatgtgtg gccttcataa tttttcaaat ttggaaaatc
    52741 ttttgtattc aatgtatagc gttcttccgc atctagtacg atcgtagact cgccatcata
    52801 gttttcaatg atttgtggat tgactcctac tgcattaaaa tctgctgtat ccatgtggga
    52861 aataaatcca atggaacgca cttcatgatc aacattgctt ggtaatgttc ccataacgaa
    52921 tccgttatct tcattataga aaacatcagc taagcctaac tcttctaatt cttttttcaa
    52981 ttcttgcgca aaagcaactt gtgtctgagt cgatggagtc gttttgcttg ttgcatcgga
    53041 gcgtgtttct gttttgacat atcgtaaaaa acgtggcaaa agattttcgt acatagtatt
    53101 ctctcctttt aattaaattg aaatgggttt gtatttgttt cagaaacgaa aaagttaata
    53161 tcccaatttt cttcttgttt ccaagattca aatttctcga taaatcgttg cttgcataac
    53221 gattcaatat aatgaccggg atcaattgca atcaacccag cgctttgcat atcatgagcc
    53281 gtatgataat agatatctcc cgtgatatag acatctgctc tttgggcgat tgcttgcgga
    53341 taaaattttt caccgcttcc accgcaaatc gcaatgcgct tcacagagga ttttgcattt
    53401 tttggctgga ctattctcag tccatctagt tggaacgctt cttttacttg ttctacaaat
    53461 gcctctatag aaatttcttg cggcaattca cctactcgtc ctaacccaaa catttcgact
    53521 ggttcatcta tagcgaaaag atcataagca ggttcctcat aaggatgagc tgaccgcatg
    53581 gcttgaatca cttgtttttc gatagtttcc ggcaaaataa cctcaacttt tgcctcttga
    53641 acttgttctg tcttccctac tttcccaatc gctggttgtg caccagcttc cggagtaaat
    53701 cgaccgtgtc caatcgaggt aaaacttgta ccggtataat caccttgtgt accagcacca
    53761 gctgcagcta atacttcacg cattttctgt gcatgatcca caggtacata aacggccagt
    53821 tttttataat gaatctcatg agtctttact aaataactct ccacttcaat accgagaagt
    53881 tcacaaaacc agtcattcag tccatcccaa ataatatcca tgttcgtgtg tgctgcatag
    53941 acggcaatat catgtttcaa caaatctgca tacatttttt cttgtggctg atcagtaacc
    54001 agccgtttta caggacgaaa gattggagga tgtttagcaa tcagcaaatc gatttttttc
    54061 ttgattgctt cttcgaccac ttctggacga acgtctaatg tcatcattac tcgttggatt
    54121 ggcttatcca atgttccgat atgcagtccg actgggtctc cttcttcagc tagccactga
    54181 ggacaatagc tttcaaactt ttggatgaaa gtgcgtccgc tgattgtcat ggctttaaaa
    54241 cctcctcaat catttggatt tcctgctcga tttgtttttg acgtgcctct tgattagctg
    54301 cttgtttgag ctgttcacgg atagcttttc tttgttgaag ctctcttgtc cattttgatt
    54361 tgaagatctc acttttctct tcaagcagaa atggcccgaa catcagctcc atttcattgt
    54421 aagtaactgg tttttgttct ttttcggcta caatgatttc atatattttt ttattttctt
    54481 caagtatttt ttctgattgg attttatact gattttcttg cagccaagat cgtaatgtac
    54541 gctctccgat gtttggctga aggatcaaac gttcttgtcc gcttaagcgt tgattcaact
    54601 tgccagcttc taggatatct cggatcagtg aacctcccat acccgcaatc gtgacagctg
    54661 taataccatc ttgtggctgg atagcatcta gaccatttgc aaggcgtacg gtgatacggt
    54721 cttctaatcc atcttttttg acttgcttac gtgctgattc ataaggacct tgaacgactt
    54781 caccagcaac tgcatattcg atttttcctc ttaatatcaa tgcaacgggt aagtaagcgt
    54841 gatctgatcc aatgtctgct aaacgagcgg attcagggac aaagtcaccc actgcggcta
    54901 agcgctgaga taaatctaaa tggttcatag cttcactcct tctagataat tatagcattt
    54961 tccaaataaa ataaggagac taggaataaa aaaattccca tatctcctta aaaaatatac
    55021 cttttcgttc tataaaacaa cattttctta tttttgaaaa aatgttattg gttatttgtt
    55081 caatgaaaca tcaataatcg aatctccagg aacttgcggt gggatagaca tcgtcatgat
    55141 cggcatctca actaatgtaa attggacttt gtcaccgatt ttcaattcat ttatgtccac
    55201 gcttattttt tcagcagtag catttaagat cactccgtca ttgctgaatg acgttcccat
    55261 attcgctgga tcttctttgg ccatgacatc gacgagtttc aactgaagcg tttcttcttc
    55321 tgcttctact tgagtgatac tttcaatcgt tccgacaaat atgctttgtc tctccgtttt
    55381 cgtactgctt gtttttactg tttgacccgc atcaatcgaa tcaacagaac ctgtgttgct
    55441 atctgcgcta ccgcagcctg ctaaaagaaa aaatagacca actacagata tactagaaaa
    55501 aatagccctc ataaaaaatc ctccgtttaa gtttctgcat tacttgttgc ggtgattgtc
    55561 tccggtcatt tctgtccata taatgattcg ctgacctgct tttactacta ggtcaaatgt
    55621 cagttggact tgtcaactgt tcaaccattg cgtcggttgc gaccagtcat ttcgatttct
    55681 tttgtcaaat aacgtatgcg ttccattatc cgtttcgctt ttagcggctc ttcatctcca
    55741 cgttgagtga cggacaaatg ctgttccaat tcatccattg taaaattcga tgagaagaag
    55801 gttggtaact gttcctgcat ccgatattga aggataacac cgaatacttc gtctcgtatc
    55861 caactactca tcgcatctgc accaatgtca tcgatcatca aaataggtga acgtttcact
    55921 gcatctaact tttctgccac ttgatctttt ccgattgcct gtttcatctc aacggcaaat
    55981 gtcggaaaat ggactagcgt cgtggtgaat ccagcaatcg ctaattctct cgctgttgct
    56041 cctaataaat atgtttttcc aataccaaaa cttcctacta gatacagtcc tttatgatac
    56101 ccttttggat cagcagtata tttctctaca aaatctaggg cttctaagct cgcatatcct
    56161 cgtccatctg tacgttcaaa gttttcaaaa gaagccgatt gaatatcttt tggcatatcc
    56221 attgctttta ctcggcttct gatttcttct tgtctttgcc gtgctaacaa tgcttccgtt
    56281 ggtacataag tgacatccac ataatgaaaa ttcaacgtca gcttcggctc atagccggga
    56341 gcgatcatcg ttggatcgtt tttttgatat ttacgctttt cttgcacgaa ttcatacaat
    56401 ttagcgtaac ttttttcaat atccgcttgt gtcaattctt cttggtgtgc gcttagaaaa
    56461 gcttgaacat ccgcatcatt taagacttca gcgatcattt cagcatactt ttcttgataa
    56521 ttgcgtttgc tgatgatcct cttcaattct ttgcctacgt cttccactag atatcaccct
    56581 ccttttttgc caggtattct ttaaaacggc ggtcaatctc tgcttttttc tcgctgctga
    56641 tctgtttttc atcttttggc tggttgaccc aatcaggaag cttttcccgt cgaacatttc
    56701 gcttgccagt ttgataattt gtttgcttct tttgttttga ttgttttgct tgattactta
    56761 tttgccgaat gtgctggatc gcttcttctg ctgtggcgat tttcttttgt gcccattcat
    56821 ttgccactgt atttacataa ctggcattta agctagcatt attcttgatc actagtacat
    56881 aattcagcaa aacattgatc acgctactgg aaagacctga ttgagaaaca agatcttgca
    56941 gtaaccagtt ttcttgttta gaaacatatc cgcctttttc tgacttgatt gcttccagat
    57001 atttcatcgg tggaatgctc tgtgcttctt ttatcagctg aagcgctgcg tttgtcaaat
    57061 gttcgtttga cgagggttca ttactgtttt cagaaaccgc aactgtttct tttgtttgct
    57121 tcaccggacg tctctgcatt gccaaaatgg ctttttgcag ttctttttca gatacttttt
    57181 gttcactaaa gtcataagat tgactgatca gttcgccaag tgtcaattca tcgaaaccgt
    57241 aaagctgatg atacatgtcg acttgtttga tgattggatc atcaagactt gcaatatgtt
    57301 tttttgccag ccaatcattc aaaaactgcc aatcgaatgt cgaatctgct tcaagtaatg
    57361 ctactgattc agattcttca aaagatttat tcagcgattc gatgttttct tgattagcag
    57421 cgtacactgc ttcattgaag cgataaacat ctaaaaattt ttttgtgatt ttttgatatc
    57481 cctctgctgt atgcctttta ggttcaaaga attgggtaag cttctggaat cgtcgttcac
    57541 caactttttc cagaagaaga aaagccagaa cagagtcttt gaaaaatgct ttaggaatga
    57601 gtggttctaa cagctcataa ataaaacgag gtcccagatc actatcttct ttaaaataaa
    57661 catccagtaa gccgattcct tctagctttt ttcttgcatc ataaaattgc gggataccgc
    57721 agcctagact actaagcaga tctgcatgca tcccttcagg acctttgcca gattcttgag
    57781 agatttctga taacagggtg aaatagatac tcaaggcttc tccgccgatt agtggctgat
    57841 aaagatacag caaggcgttc ttgctgtctt ctctcagcgg agccgctttt cttccttggt
    57901 aaatattttt tggttgaagc tctttccaag cattttccaa ggaaatgcct cctttttgtt
    57961 tcttaattgt tttctttatt attctcttta ttattttctt ttgctttatc cacgatatct
    58021 tgcaactctt ttaagaaaac agacatatct ttgaactggc gataaacact tgcaaaacga
    58081 atataagcga tttcgtctaa atcaactaaa tcttccatca catattcacc aattagattc
    58141 gtcgatactt cgttttctcc gagactgcgt acgcggtttt cgacatggtt gacgatttgt
    58201 tccatttgtt ccatagctac aggtcttttt tctgctgaac ggatgagtcc tcttaaaatt
    58261 ttatcgcgat tgaactcttc gcgttccccg tttttcttga ttactaataa cggagcagct
    58321 tcaattcgtt caaatgtagt gaatcggaac gaacaattct cacattcgcg acggcgacgg
    58381 atcgcacgtc catcatctgc ttgacggctg tcgattactc gtgaattatt atgttgacat
    58441 ctgggacatc tcattttgtc tcacctcttg catcgcattt tatataaagg atgtgtcttt
    58501 ctttacaact agttgtatta taacacgaaa caaatcacaa acgcgactgt cctacccaaa
    58561 aaacacggat ataagaacaa ttttttctac tttcatgcgt aattgtgtcc cctgtcctta
    58621 ctaaatttca attttccaat actgatcacc ttgattactg agaaataact tacacaaaag
    58681 ctgatttagt catctagcac aaaaaacttg aagcagatga acaccttctc atctgcttca
    58741 agtcacatct cgatttttta ctattttata acttctcttc gattcctgta tagtgtttca
    58801 ttcgctgttc ctcgattttt tgctcatcgg gaatgtggtc actttctggg agcggtgttt
    58861 tactacgatc aggtgtcgga ttttttcgtt cttgagtcaa atcttgccgc ttcgcttctt
    58921 cttctgcctg ctggtcttct atttcatcga ttcttgcttg gtactgcttt tctttttctt
    58981 ctgctgtttc tgattgcgta cgaccttcat cgattagatt ttctttattg agcatctcgc
    59041 cgattttttc ttttgcgtta cctacgactt tatccttttg attttttaat gatcccatcg
    59101 ctatccctca ctttctatat actcaggata gcaaaattga cacagatact caaagaaagc
    59161 gattagcaaa aagccaattt tcgatttgtc ccacaagtat ttcttttgtt ccattgttgt
    59221 cgaacaaaat atccgcacgc tgcttttttt cttcgattgg aagttgacta ctaattctct
    59281 gctgcgcttg ttctagcgtc agttgattac gtttcatcaa acgctgaagc tgagtttctg
    59341 gcgtagtata aacgacagct actgcatcca tatagtggtc ataatgacct tcgtagagca
    59401 atgggatatc tacaattact aaaggatgat tggaacttag agaggcgatt tgatctttta
    59461 tcgcttttcg gataaacgga tctaatagct gatcaagttt ttgccgtttt tcctttgatg
    59521 gaaaaacaat catcccaagt ttccctcgat ctagttgccc atcaggctgc aatacctctt
    59581 ggccaaacac tgatacaatt ttcctcagag caagcgttcc aggctcaaca gtttctctgg
    59641 caatcacatc gccatcgatc aaaggaaatc catgagactg aaagactttg gctgccgtac
    59701 ttttaccggt agctattcca cccgtcaaac ctaaaacaaa tcctactttg cgatttttag
    59761 cctttgacaa ttcggacaat agtgcgtccc cctttgtgcc acttttgttt tcacgatcgg
    59821 cgttccacaa cgtatacatg gtttgccagt ttgctgatag acatgcagtg ctacttgaaa
    59881 tccgcctgat tctcctaatg cattcaaata actgcgaatc gtggtacctc cggcttctat
    59941 tgcacgatcc aaaacatcaa tgatcgatag acgtaattgt tctacctctt tggggcgcag
    60001 tgtgttcgcc ggttgttctg gatggatctt cgcttcccat aatgcttcat ctacataaat
    60061 attacctaga ccagtcacta gacgttggtc tagcagcaat ggcttgattg ctttgtggga
    60121 ctttttcagt ccatttgcga aatctgctaa taagaagcta tctggcaaag gctcaggtcc
    60181 caatttcatg attcctcggt aagttgcact tgctcctttt tcgacgattg tcatacgacc
    60241 aaattttcgt acatcgttgt agcgaagttg gcttccatcc tcaaaaaaga aaagtacatg
    60301 cgtatgttta tcgattggtg tattttcttt cgtaaattga tattttcctt ccattcgcaa
    60361 gtgtgagatc agttcacaat gatcgagatg aaaaattaaa aacttccctc tacgtccaat
    60421 ggattggatg gtttcgccaa caagagaagc ctcaaagatg ggtgtctctg gttgctcgat
    60481 gatcttgggc cacaagacct gaactttttg aatcgttttt cctactacta accgttccaa
    60541 tcctttacga acggtttcta cttctggtaa ttctggcatt ttttcttttc accttcatta
    60601 tattgctctg tcttaaacaa tgtttatttt gcttcgtacc acgtttttcc ccaactgctg
    60661 tcagtgatca gtggtacatg aagagaaaca gcttgttcca taacttcctt caccaattta
    60721 tctaatgatt caagttcttt ttccggtacc tcgaagacaa gttcatcatg aacttgtaac
    60781 agcatcgtag cttgaagacc tgtttctttt aatcgtttat ccaattcgat catcgcgatt
    60841 ttcaaaatat cagcagcact tccttggatt ggtgtattga tagctgttcg ttccgcaaaa
    60901 gaacggatat tgtaatttct tgaattgatg tctggcagat atcgacggcg gtgatacagc
    60961 gtttcgacgt agccttggtc ttttgcttcg cgtacgattc tttccatata ttctttgaca
    61021 cctgggtatt tttcaaagta cgtatcgatg tattgctgtg ctgcttttcg gctgattcct
    61081 agattttgtg aaagtccgta atcagagatg ccatacacga taccaaaatt cactgctttt
    61141 gcctgcctac gcatatttgg tgtgacatcc tctgcctttt cgattccaaa tacacgcatc
    61201 gctgtgctgg catggatatc ttgaccttct aggaaggcag ctttcaaatg ttcatcattt
    61261 gagatatgcg caagtacgcg taactcgatt tgtgaatagt cagaagaata aatcagccag
    61321 tctttttctc taggaacaaa tgcctgacgt atctttctcc cttcatctaa tcgaatcggg
    61381 atgttctgca aattaggatc aacagagctt aatcgtcctg tttgggtcaa tgtctggaca
    61441 taacgggtat gcacttttcc atctgattgg atcactttca gcagtccctc gacataggta
    61501 gattgaattt ttgcaatttg acgataggtg aggatatcct ctacgattgg tgcttgttca
    61561 cgaagctgtt ccagtacatc tacggctgtg gaatatccag tttttgtttt tttgataacg
    61621 ggtaatccca ttttttcaaa taagatcact cctaattgct ttggtgaatt caaattgaat
    61681 tcttcgccgg cttcttcgta gattttttgt tcgatctctc ttaagcgagc agagaactcg
    61741 cccttcattt cttgtaagcg agtcgcatct actttgatcc cgcttatttc catctctgcc
    61801 aaaatgatag acaatggcag ttccattttc cggaaaagat cttcttggtt tttttctgcc
    61861 agttcttggc tcaattttgg tgtcaacgca ttgattgcag ctacttttcg cgctaaatga
    61921 gcaaaaaaga gttcctcttc ttctggtaaa ccttttttcg cccctttgcc gtatacagct
    61981 tcatctgatt ggatatcgtt gtatccgtaa tgagcagcta ttccttcgat atctgtgctt
    62041 ttttcattcg tatcgagaag gtatgcacct agcaagacat caaaatcgat tcctttcgtt
    62101 tttccagtat agcggtttaa agcaacataa gttcgtttcg catcatatac ttttttcagc
    62161 cgagtcggat cagtgatcca agaatggaac gctcggtttt cgaatacaga tagatcgttc
    62221 gtgacataaa ttttcttttc tgtcccccac gctactccga cgatttcctc tgtatgataa
    62281 ttgtcgccca tcatttccac gtacaacgcc atatccgtag taaacatttc ctctttgaat
    62341 tcatgaacga cttcaaacaa aatatcatcc atttttactt cttcttctac gatattcaat
    62401 ttagaaagga actgattgaa atccatttct ttataaaacg gaacgagttt atcgagattt
    62461 ttcccttcat acttcaatga gtcgatagag acatccacag gcgctgaagt attgatggtc
    62521 gccaattttt tcgataagaa agcctgctct ttgtcattta tcagattttc tttcattttg
    62581 ctttgcttca tttcatcgat atgttcataa attccttcga ctgagccata ttgtttcaat
    62641 agcttgatag ctgttttttc accgatttta gtcacaccgg ggatattgtc agaggcatct
    62701 ccggctagtc ccttcatgtc aataatctgt tttggcgtca acccatcata tttttccgct
    62761 acatgttctg gtgtatagct ttcgatatcg ctgacacctt ttacagtgat atccactttt
    62821 acttctttac tagctaattg agtcaaatcc cgatcacctg aaagcacgac gacatcaaat
    62881 tgatctttgt ctactttttc tgccaaagta ccgataatat catctgcttc ataattgggc
    62941 aattcgtaat actgtacccc tagtcctgtc aacaaatcac gtatatatgg catttgttct
    63001 ttgaattcgc caggtgtttt ggatcttcca cctttgtact ccgcatacat ttctgtccga
    63061 aaagtcgttt ttcctgcatc gaacgcaacc agaacatgag tcggttgctc tttttgcata
    63121 acattttcaa acatcgtatt gaatgcatag attgcatttg tatgaagacc gtttctattt
    63181 ttgaaacgtt caagagaatt gtgaagcgca aagaatgcgc gaaaagccac actgttccca
    63241 tcaacgagta ataatttatt ttttgtcatc ttcttaactc cttactgtaa agttcttatt
    63301 cattctaaca aagaatcctg cggaatgcga aaagaaaaag cgggaccata ccaaataatt
    63361 cggtatagcc ccaactgatg aatttttttc ttttttagtt gttccgtcca aagattctca
    63421 gaaacgccaa gaacaagttg ataaagtcca aatacagctt caatgccatg aatacagcaa
    63481 ttccactatg tgctgttccg tttgtctgga catagatatt acggatgctt tggttatcat
    63541 aagctgtaat ccctgcaaag atgacgacta acagtaggga gatgaacagc tcaactacgc
    63601 tacttctcaa aatgaagatg ttcaagacta gtgcaatgat cactccgatt aaagctgcct
    63661 gtcctgctct tccaaccccg ctaagatctc tttttgttgt tactccgaca acagacatcc
    63721 ccatgaacat caaagtagct gtcaagaaag cttgcgttac tgttccaata tcgtagtatg
    63781 caagagtaac cgctaatatt atgccattca aaagagagta ggcaataaat ccaccgattg
    63841 ctaagctcgg attcttttgt gcttttacgc ctagaaccat tactaaaatc agttcaacga
    63901 tccacagacc cacaaagcct agtggaaaat tattgataaa agttgctaat tgcaaaggaa
    63961 atacccaaag agccaagtaa gaagtgactg cactaagggc aagtcccata cctaaaaatc
    64021 cataaacttt tgcataaaaa cgattgatac ctgacattga tgtacttgga ttattcattc
    64081 aattcacctt cactttcgat tgctatttcc aattcaggaa cttgtggcgg ttggaccata
    64141 acgacagcat ccaacacacg atcttcgttc gtattctccg cttctacaga aatcgtcacc
    64201 acatccttca tcttatctac ttttatgacc tcaaactgaa aagtcagtgt ttcataatgg
    64261 aaaaccggtt caataaaatt gacagaaaaa ttgacaacat gtgatcctgg tccaggaaga
    64321 tgctttgaaa ttgcacttgt gatgatcccc atcagcatca ctgatggaac gattggctgt
    64381 tgatattctg ttttttgtgc aaagtcatgt tggatataca aaggatttgc atcattcgtc
    64441 aatcccaaat acagcaaaag ctggttatct tcaatagatt ctgttaagga taacgagtcg
    64501 ccttcctcga tttcatttat tgtttttcct agttttctcg gcttacctat gttcaagata
    64561 gaaaccctcc agtttatttt taatcataca ttattgtacc aaaagtagcg taaaaggcaa
    64621 gttctgctcc cttgatttaa cagattccta tagtttacag aggtcgtgaa aaagctgtcc
    64681 tgcatcaaat aataagaaca gccaagaaaa aacagcttct catgtttttg atttgattgg
    64741 gcttattacc gcagatgcaa gcttttgaac accgtttatc ataagttgca aaaaaggggg
    64801 cttggcaaga tttttgccac tccccctctt tctattttta taaggacact gcagtaattg
    64861 tattacagta catttttcgt cgtgctgctt aaaagttttt catacgcttg ttcaaatttt
    64921 tgaacatctc cagcacccat gaagattact actgcatcat gataatctaa caatggagat
    64981 acattatttt ctttgatgac ttctccacct tttttgattt tcgctccaag gtcttcaatc
    65041 ttaacgtttc cttgttcttc ccgagccgaa ccaaaaatat cgcataagta aactttatca
    65101 gctaaatcaa gtgcttctgc aaattcatcc atcaatgcaa tcgtacgcgt aaacgtatgg
    65161 ggttggaaaa ctgcaattat ttctttatct ggatattttt ggcgtgcgcc atcaatcgtt
    65221 gctttgatct ctgcagggtg atgcgcataa tcatcgacta cggtcatgtc tgctacgatc
    65281 ttttcactga atcgacgttt tactcctggg aaagtcagca tttcttctgc aacttctttc
    65341 agatccagtt tttcgaaata agctactgca ataacaccta aagcatttaa gatattgtgt
    65401 ttaccgaatg ctggtacagt gaagtgccca acaaattcat ccccatgata gacatcaaaa
    65461 gcagaaccag aagttgtgcg ttcgatgttt cgtgcttgga tatcatcgtt ttctgtgaca
    65521 ccataataat aaatcggcac atttgctttt aatttgcgta aataagcatc gtcaccatac
    65581 gcaaaaatag cttttttcac ttgtccagcc attgtttgga aagctgtaaa tacatcgtcg
    65641 atacttgtat aataatccgg atgatcaaaa tcgatattgg tcatgattgc atagtctggt
    65701 gaataggcaa ggaaatgacg gcgatattca caggcctcaa atgaaaagaa ttccgcttgc
    65761 ggatcaccat gtcctgttcc atctccaatt agataacttg ttggacggac accactcaat
    65821 acatgagaga gcagtccagt cgtacttgtt tttccgtgag agcctgttac agcgatactc
    65881 gtataatttt ggataaaatg accaataaag tcgtgatagc gaatcacttc caaacctaat
    65941 tcttttgctc gctggatttc ttcatgtgaa tcaggaaatg catttcctgc aatgatcgtc
    66001 atccctggtt ttacattatc tgcgttaaat ggcagaatcg tgatatttgc tttttctaaa
    66061 tctctttgtg taaagaaata tttttcaatg tctgatcctt ggacatttag accttgttca
    66121 tgtaaaacaa gagccaagga gctcattcct gaacctttga taccaacaaa atgatacaat
    66181 ttattttgat tttccatttg aatcctcttc tcttatctag cgtccattct tttagctatt
    66241 acattcaaca cgctcctcat tatcttataa acgattgaaa atttcaacca aatttatttg
    66301 gaaaggagga ttcgcttgct aaaatggtca agtacgagaa aaatccgttt ttattgactc
    66361 tttaattctg gtgtaagaag accaaacttc ctctgatata tcaatgtgag gaataaaatc
    66421 ttttcctttc ggaccatagc cgttctcatc gtcatggaga cgttccattt ctcctaatac
    66481 tagcgcatgg aaatattcct taggccattg atgattcagt tcttttgctg taaagatgat
    66541 ctcttcagaa aagtgaagga tttccggcac aaatggcaca gcattcgcga aaatagtttg
    66601 ttcattttgc catactttct caaaagttgc tcgagtcctg cgttgcagtg tcggatcatt
    66661 catcaataag attttttctg gtacagctcc taaggaatga agaatctcca gtgaaaaagt
    66721 ggcgttctct ccagaatttg tagacttcga ttctatcaga aaagccttat cgggcaaatc
    66781 atagacttct tttagatact gacgacagat ttcactttca ctcatgccat cttcaaaatg
    66841 aaatccttgc ttttcaaaat tctcataaag gattctagtc gcatgcccca cccctccgac
    66901 caaaaacaat tgatctactt ggccatttaa atacatttgt gctgctttat cagctaagat
    66961 aggaagacag tttccagcta aaataagtgt aggtgccgtc agttcctcaa aatttccctc
    67021 ttcaggtgcg gctaaaaaat ttaatagctg attccagtct tttagattcc tctgctcact
    67081 tttttccaaa ggccttctat cctttcatct gattttttgt gcttcattaa agtcttctct
    67141 ttttgtctca atccatttga tttctccatg acatcttcca caacgatatt tgcgtacatc
    67201 gattcttctc ttgcggcaga tcgtggcaga acaatcttgg catcgataat actccatctt
    67261 tatctgtttc ttcgtcaagg ctggtgcata gcgcaaccct cctgtttttt tcaaaagata
    67321 cttgaaatcc atatctttat gctgataccc tcttccagct agatgaagat gataatggca
    67381 aagttcatgt ttgatgattc ctgcaatcgt ctcttcgtct gactcttcaa atagcttcgc
    67441 attaaaatct aaatgatgat cgtttaaatg atagcgcccg ccagttgtac gtaagcgtgg
    67501 attgaacatt gcttggtgct tgaaaggaag ctggaagctt tttaatgata tctcttctac
    67561 taactgctgc agttcttctt gtgtcaattt ttgttactcc tcattgttac tcttcataag
    67621 gggaaagcat cgtcaaactg attctgcctt ttttcgtatc gacttgttct atccaaactg
    67681 ttacgatatc tcctactgac acgacatctg taggatgctt gacgaatttt ttgctgagtt
    67741 tcgatatatg gacaagtccg tcttgcttca caccgatatc aacaaatgcg ccgaagtcaa
    67801 tgacattacg gaccgttcca gtcaattcca tccctggttt caagtcttcc atactcaata
    67861 catctgtacg taataagggc gctggcatct catcacgcat atctcgtcca ggttgtgtca
    67921 gcccagctaa aatatcggct aacgtttctt ctccgactga taggctttca gccagtttct
    67981 ctacggataa tcgtgtcaat ttttctactg cttcttctgt accaagctct ttttctgaaa
    68041 gttggactga agttaatatt tcttttgcaa tagaataact ttcgggatgg atacctgtgt
    68101 tgtccaaaat gtttttccca ccagggactc gcaagaatcc gattgcttgt tcatatgctt
    68161 ttggtcctaa gcgtggcact tttttcaatt gagttcgtgc ggtgaattct ccgttttctt
    68221 cacgataaat gacaatgttt tgggcagttg ttttattcaa tcctgatata tgctgcaaga
    68281 gctgcgggct tgctgtattc acatccacac ctacttggtt aaccgctgtt tccacaacaa
    68341 aatccaattg ttcagccaaa cgtttttgag aaacgtcatg ttggtactgg cctactccga
    68401 cagctttagg atcgattttg actaattcag ctaaaggatc ttgcaggcga cgggcaatag
    68461 aaacagcact tctttcttca acttgtagat gcggaaattc ttttcgtgca atttcgctag
    68521 cggagtaaac agaagcgcct gcttcgttga cgatagcgta ataggtttta tggtcggcgg
    68581 atttcaactg ctcagccaca aaaagttcag attcccgact ggctgtacca tttccgatag
    68641 ctaccatatc tacttgatat ttattaatta attggataaa agctggtcca gcagcttctc
    68701 tttttgcttg cgctgcaggt ttatgaggat aaataacttc gattgctaaa actttccctg
    68761 tcgcatctac gactgctagt ttacatccag tacgataagc cggatcaaat cccaggacaa
    68821 ctttaccttt tagtggcggt tgcaacaaaa gattacggag gttttcgccg aaaatagcga
    68881 ttgcttgctc atctgccttt tcagtcaatt cattgcgcag ttctcgctcg attgctggct
    68941 gaataaagcg tttataacta tcctgatagg cttctctgac gaatgaagaa gaaggagagg
    69001 caggattttt gactatttgg cgatccaagt aagctaaaat tgctgcttca tctacttgaa
    69061 agaatacttt cagaatatct tctttttctc cacgattagt agctaatatt cgatgagaaa
    69121 ccattttatg gacgggttct gcaaagtcat aatacatttc atatacgcct ttttcatctg
    69181 cttgctcatc cttgacattg ctgacataca tacctttgtt atacgtataa gaccgaatcc
    69241 aagtccgaaa ttttgcatta tcgctgactt gttcagcaat aatctcatga gcaccgtgca
    69301 acgcttcttc ggcagaagag acttcttttt ctttgtcaat atatttttct gcttcagatt
    69361 gcacctctcc atctgtaagc tgcatcagcc acttcgcgag tggttcaagt cctttttctt
    69421 tagcaatcgt tgcttttgtc cgtcgttttt gtttgtatgg acgatacaaa tcttccactt
    69481 gttgcatttt cacggcttgg gtgatttctg cttctaactc gggagtcagt ttcccttgtt
    69541 catcgattag ccgaatcact tcagttttac gtttttctaa gttttcaaga taggcatacc
    69601 gctcttcgat ttcacgaatc tggacttcat caagacttcc agtcatttct tttcggtaac
    69661 gcgcgataaa aggaacagtg tttccttcat ttaacaaatt aagtacagta gtcaactgct
    69721 taggacgata gtctgaaagt tctttttgta caagctgaat gatcgtctgg ttcaaatttt
    69781 ccgtcattaa aatgcctctt ttctgctaaa aattgcttct ttattctatc atacttttta
    69841 tcctgggtta aactactgaa agaaacaata ttctctataa aaataaaaag agccgcaaca
    69901 aagtcacagc tccttcattc tttccattag tcagattcca tgccaaggtc cttcgttgtt
    69961 ctgatattca atatcttgaa gaataccttc cttgtcaaaa acaaccccat gcaatgaccc
    70021 tccgaataca gccccaccgt ccattccgat tttatgatcc tgaatccaga ggtctgttgt
    70081 ctgcatatct ccatggagca ttggtgtaat cgtatgtcca aatacaattg tcttacctgt
    70141 attgtttttc ccttcatgaa atggttccct tatccaaata aagtctctta gacttgtctt
    70201 acgccagtct ttctttgtca aatccacacc ggcatggaca aaaagataat tttcccattc
    70261 ataatacaag gggcgctgga ctaaaaattt aatcagttct gaatatctcg attggatcat
    70321 catcgagatt tcagttgggg agtattcctc cgatgcccct gggtgaatca ggctttcaaa
    70381 cgtttctttt ccaccatttc tcatataagg cgcataccag tcctccggtg attgcagaaa
    70441 ttgcaaaaaa tattcttcat ggtttccccg gaggtaaatg gctcctgttt cttcgactaa
    70501 ttgctttcct aataaaaaac attcttttgt acgaggacct cgatcattga gatcccctaa
    70561 taaaacaagc tgatggatct ttggatcata atagtgaata agtttttcaa aaagctcatg
    70621 tttgccatgg acatccccaa tagcataaat caatgatttc atgtggatca ccatcctcaa
    70681 cgctagtata gcgcatattg gaaatggtcg acaatcaaaa ctattgtttt ctatttaatt
    70741 aaagctgtaa accgcagttc tgcttcttct ggagagattc catggtcttg aacatagcga
    70801 ttgcgcggat gtttcgcaca ctctatgcta catgctccta aatgtttttc ttcattttca
    70861 atggaagcaa gaatctgacg attgcactca ggattgctac aattgatata tcgttcacat
    70921 ggtgttccgt caaaccagtc gcgaccgacg atttgtttat ctacttggtt gatatcgaca
    70981 gcgatccgtt catcaaacac atacatcttg ccgtcccata attctccttg gacttcagga
    71041 tccttaccat aagtagcaat accgccatgc agctgtccga catctttgaa tccttcacgg
    71101 acaagccaac cagaaaactt ctcgcaacga atgccaccag tacagtaggt cactacccgt
    71161 ttttccataa attcttcttt gtgctcgcgg atccagtctg gaagctctcg aaaactgcgt
    71221 atatctgggc gtacagcccc tcgaaaatgt cccaagtcat attcataatc attccgtgca
    71281 tcaatcacca ctgtctgttc atctaaaata gcttctttaa attcttgcgg tgaaagatag
    71341 gcacctgtca cttggtttgg atcaatgtcc tcttctagct ttaaacttac aagctctgga
    71401 cggtatctta caaacatctt cttaaaggca tgtccttcgc ttggatcgat cttgaacatc
    71461 gtatcagcaa atcgaggatc agctttcatt gctttcatat aggcagtcgt gttttcaacg
    71521 gttccagaca cagtaccatt tattccttct tcagctacta gaatacgacc tttaagatcc
    71581 aaagatttgc aaaaggctaa atgatttttg gcaaattcct ctggttcttc gatttttgta
    71641 taattgtaat aaagcagtac ttgataatcc atgtatttct tccttccttc ttttcgttcc
    71701 taaacagtat aaagaaggcg ttcgcatttg acgatttatt tgcttgtgta taattacgca
    71761 tggaaagttt tttgtttaca taatattatt atttgaatta aaaagacttc gctttcctgt
    71821 ttcgcacaga aaaacaaagt ctttatttct attatttacc tgtaaccgca aagtagttgc
    71881 catcgggatc agcaaagtta aagacatatt cttctccaag ctgtaccata tcacctaatg
    71941 tgactgattg cgtctgcatt tttttatata gtccaaagat atctttactg taaaacatca
    72001 aagatggggc actagtagct gtgccaggtg aatgggactc aacaaatgct cgatcatata
    72061 acagaaatga ggtttgactg tcctcaactc ctacttcaat tataagcgtc ccatccattt
    72121 cttgacgatc tttttccgca aagccaactg ctctccaaaa cgttgcattt ttttctacat
    72181 cgtccacata aagcatgatt tttatttcat tcgtaaacat ctgtttcctc ctaaacgatc
    72241 tgttctaaca ttctcaactt tgtcagttta ccataggaaa atgggaaaat ctacgaaagc
    72301 tctcaacaga atcgtcctac ttaaatacgc ttttctttaa taataaataa gttggttgtt
    72361 tttgttattt gcgctttcac ttttcggaat aaataatgtg tatttatatg gaataaataa
    72421 aaaaagaggt cgtgactaaa aacaagtcac aacctctggt aaacggtgtt ctcctgacaa
    72481 ctcctcggtc acaagacaca atattcaaca aacatgagaa gctatttttt gaaattgctc
    72541 tttgtgactc agagtgaaac ggcaggctca cgacctcttt cttaaacggt gttcaaaagc
    72601 ttgcatctgc ggtaataagc tcaaccaaac gtaaaatatg aggaactatt tttgtttggc
    72661 tgttcttatt acttgatgca gggcagcttt ttcacaacct cttattcacg gtgtttcaga
    72721 agtagcttct cggaaataag ccgaaatttc acaaaaattt gagaaactat ttttaggaaa
    72781 cttcttctta tttcttaaag ctgaccactt ctatcacaac ctctttttga ctatgcattc
    72841 acatcgatca ctttagaatc atcattgatg acatcgcgat ctaaatcttt gatgcgtgca
    72901 cttgtcacga taccagctac catgctaccg ctgacgttta ctgctgtacg tgccatatcg
    72961 attaatggtt caacagagat aactaaccca acgattgcta ctggcaagtt cattgcccct
    73021 aaaacgatca gtgaagcaaa tgttgcgccc ccaccgactc cagctacacc aaaagaactg
    73081 attgttacaa ttgccacaag catcaaaata aattccaaac taaagacatt cactccagct
    73141 gctggtgcga cgatcgttgc caacatagct ggataaacgc cagcacagcc gttttgtcca
    73201 atagacaaac caaaacttgc tgagaaatta gctgttgcat catctactcc taaagctttt
    73261 gtctgagtct cgatattcaa tggcaaagct ccagcacttg aacgtgaagt aaacgcaaag
    73321 ctcaataccg gaaaggcttt tttgaaatag ttgattggat tgacttttgc accaatcaaa
    73381 atcagtgagt ggacaatcaa aacagcaagc aatgctacgt aagaagcaac gacaaattta
    73441 cctaaattga cgatagctgc aaaatcactt gttgccaaaa cgtttgtcat caaagcaaat
    73501 actccataag gtgtcaaacg caaaacaagc gtcacgatcc gcataatgat tttatacaga
    73561 ctgtcgacta aatctgaaaa gaatgctgct tcttttggcg cttttctttt cacgcctaga
    73621 taagcgattc cgacaaatgc cgcgaaaata acgactccaa tcgtacttgt tgcacgacta
    73681 cctgctaaat cagcaaaaac gtttttaggg ataaaggata aaatctgctg tggaatcgat
    73741 aagttttcaa ctgtttcctg acgtgttgcc agttcagaga ttcgtgctgt ttctgcagct
    73801 ccttgtgtga atttagctcc ttggagtccg aacagcattg ttgaaccaat tccgatcaat
    73861 gctgcaatag ctgttgttcc aagtaaagaa gtcaatacag tcgcactaat tttccctagc
    73921 ttttcagaaa ctttcatttt tgtgaatgca ccaatgatcg aaataaacac taaaggcata
    73981 actagcattt gcaagaatga tacatatcct tgacctacaa tcccgatcca ttccattgat
    74041 tgagtaacga ctttgtcgtc tacaccaaag atcaattgaa gcagaccgcc aaagacgata
    74101 ccaactccta atgctgtaaa gacgcgtgtt gaaaattttg tatggcgctt ttgcatacga
    74161 tagaacacat ataataatgc cgcaaaaact agaaccacta aaactgtgat gagtgttgtc
    74221 atttttctcc tcctcatatt attaatgaca agtccatcga aggaagtaaa ggttcccgag
    74281 aatgattctt caaccatcct cctgaaaaaa tttttttcag tgacttttat attttagacg
    74341 atttttatcg aaatgacatt ctttttgctt acaattagta ataaaaaaac atacctgtga
    74401 tagctttaat ctatcgcagg tatgttttta ctgtctattc ttgtttttct gattgaaaat
    74461 tcaattttac cccaggctga tctaaggcga tctctgtcac ctcataagac attcggcgta
    74521 cgatatcgaa taacggtgct actagctcgt ccacttcttc ttgtgtgaca tcattttgac
    74581 cttcaatttt ccgattgagg atatgattga tctgactcac acttccagat aaaacatatg
    74641 tatcaaacac taaacggaac tctaagcgtg ctcctacgat gctatgttct ttcggatagt
    74701 tttcgatcgt ttcttttaaa ggcgagaagc ctactttcaa atctgtttct acttcttggt
    74761 tcggcaatct catatcataa tgaaatgctt ctacaacttc tttttgtctt ctgatttcca
    74821 tataatttta tcactccttt atgaaaatta taacatatct cctttgaaag gggatactac
    74881 tttttaatga tgctctgcaa ggtaaggatt tcattcagat ttcctaccac ataatctgcc
    74941 gggatctcac ccagaaaatg atcgatgtcg taaagacaag tagcgactcc tgcatttttc
    75001 cctgcttcga tatctaaacg acgatcgccg atcatcatcg tgtctgttct ttctaaatga
    75061 aaagtatcga tcaaataatt taaagatgct ggatcaggtt tacgaggaaa gtcctgatcg
    75121 atccctacta cttcctcaat cagatgtgcc aatcgatgtt cttttaacag gccccatgtt
    75181 gattctgtca aacggtgggt caggatgaaa tgacgaccgc cgttgtcttt tagcatttcc
    75241 agtacttgtt tcgtttctgg aaatggacgg gaaacttccg tactttcttt ttcatatcgg
    75301 tgaaataatt cattaaactc tctggcattc aagtttgatt cattgatcaa ataactagta
    75361 gaatactttt tcattttgaa gtagatttct tttttgtcca tactgatacc aaaatctttt
    75421 agtgcttgcc atgcaccatc taccattgcg ggatacgtat caaacaatgt accatcaaag
    75481 tcccatatat aatttttcat gttccttttg ttccattcct ttgcttcttt tttctatctc
    75541 tcacttttag ataaaggtgt tctttaaagt atttttttgt ccagaattgt caaatcatct
    75601 gctaatgtat actggattgg ttgtgcattt gggatatcga tcgtatccat ttgatcttca
    75661 ggaacatctt ctaaatattt tgtcagtgct cggatgctat taccatgacc cacaactagt
    75721 aaatcttgcc gatcttttag ttttggtgct agatgatctt gccataaggg aatgatccga
    75781 ttcactgtca tttccagact ttctccgtat gggatcaaat gcggatccaa gtgatcatat
    75841 ctgcggtcaa atctattttc tgcggttaac ggcggaactt ccgtatatcc tctgcgccat
    75901 gtttttactt gatcttttcc gtatatttct ttcatcttat ctttgttttt acccacaaga
    75961 tccccataat gccgttcatt tagacgccat gtttttatta taggaagcca taactgaccc
    76021 atttcttcta gtgcgacttg tccggtaaga atcgcacgtt tcaaaacaga ggtataaacg
    76081 gcatcgaatt gaagtccggc ttcaagcatt ttttgaccgg caactgctgc ttgttctttt
    76141 cctttttctg ttaaggcgac atctagccat cctgtccagt aattttcaaa atttgcttcg
    76201 ctttcgccat gacgtaataa tactaatttc attcttttct cccttaaaca ttcgtttcga
    76261 attttatacc acatgttttg catgtcacac gtattcgtcc ttttccttta ggtgcacgct
    76321 gcttttgatg acaattcggg cactcaaaaa aagtataact ttttcgatcc attttgactt
    76381 gctttttgtt ttttatctga tgatattttt ctttgactct atcaagatat ttcaaataac
    76441 gttgattctc atttagtctg gggtagattt tttttgatag gaaacgaaat atcatccatc
    76501 cgaaaaagac gatgaacaac aattgtccga ttctataggg caaccaacga cttagcaaac
    76561 taaagacaag cgcagcgatc aatagaccgc gattcaactg atcaaatttc gcatatcttc
    76621 cctgcataag tttgtgatag ttctgctgaa atcgtattag cctttctaac catgtttgca
    76681 tcatattatc tcctccatca tctaatattt attatgacaa aaaaatccaa aaagaaaaag
    76741 ccttaattgg ccttttccat tgcaacttgg tgaaggacat tccgatttct ggtaaagtca
    76801 atcacaatca ctttcttttt atcttctctg ttgactactc gaaatgtgtc cgtcaatgga
    76861 agaaacagct tcttagatgg tagctgttgc atacgtataa aacgatggat catcgtaatc
    76921 aattgaggaa attgaataga cgtgtgaagc agacctggat attttttcaa cagctgtgtc
    76981 gttttgatca gattccgttt tttactgatc acttttaaaa agctttttag aatcttttgc
    77041 tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat ttgtttttgc
    77101 agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa accttctgga
    77161 aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata atcttttact
    77221 ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc tgcttgatag
    77281 atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag caatgtttta
    77341 ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc ttttaaaaca
    77401 actaaaaaat tcatttttct cccccttttt tcatcatttg tgaactactc accacttagc
    77461 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
    77521 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
    77581 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
    77641 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
    77701 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
    77761 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
    77821 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
    77881 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
    77941 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
    78001 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
    78061 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
    78121 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
    78181 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
    78241 tacacaacaa agaaatata
//
LOCUS       DAFBQW010000007        67762 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFBQW010000007 DAFBQW010000000
VERSION     DAFBQW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.007"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(98..1228)
                     /locus_tag="LK889_000654"
     CDS             complement(98..1228)
                     /locus_tag="LK889_000654"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK5094912.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="LK889_000655"
     CDS             complement(1877..3079)
                     /locus_tag="LK889_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5094913.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="LK889_000656"
     CDS             complement(3139..3342)
                     /locus_tag="LK889_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBK5094914.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="LK889_000657"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="LK889_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="LK889_000658"
     CDS             4879..5691
                     /locus_tag="LK889_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094915.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="LK889_000659"
     CDS             complement(6715..6867)
                     /locus_tag="LK889_000659"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK5094916.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="LK889_000660"
     CDS             complement(7058..7936)
                     /locus_tag="LK889_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5094917.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="LK889_000661"
     CDS             complement(7975..9093)
                     /locus_tag="LK889_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBK5094918.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="LK889_000662"
     CDS             complement(9090..11189)
                     /locus_tag="LK889_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBK5094919.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="LK889_000663"
     CDS             complement(11240..12061)
                     /locus_tag="LK889_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5094920.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="LK889_000664"
     CDS             complement(12058..12867)
                     /locus_tag="LK889_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5094921.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="LK889_000665"
     CDS             complement(12888..13382)
                     /locus_tag="LK889_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5094922.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="LK889_000666"
     CDS             complement(13394..13882)
                     /locus_tag="LK889_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5094923.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="LK889_000667"
     CDS             complement(13959..16655)
                     /locus_tag="LK889_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5094924.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="LK889_000668"
     CDS             complement(17083..18156)
                     /locus_tag="LK889_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5094925.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="LK889_000669"
     CDS             complement(18153..18980)
                     /locus_tag="LK889_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094926.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="LK889_000670"
     CDS             complement(18981..19787)
                     /locus_tag="LK889_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5094927.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="LK889_000671"
     CDS             complement(19799..20884)
                     /locus_tag="LK889_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5094928.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="LK889_000672"
     CDS             complement(20901..21443)
                     /locus_tag="LK889_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5094929.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="LK889_000673"
     CDS             21701..22690
                     /locus_tag="LK889_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK5094930.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="LK889_000674"
     CDS             22687..23424
                     /locus_tag="LK889_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBK5094931.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="LK889_000675"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="LK889_000675"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBK5094932.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="LK889_000676"
     CDS             23988..24620
                     /locus_tag="LK889_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5094933.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="LK889_000677"
     CDS             24705..25352
                     /locus_tag="LK889_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5094934.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="LK889_000678"
                     /pseudo
     CDS             25357..26340
                     /locus_tag="LK889_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            complement(26743..28257)
                     /locus_tag="LK889_000679"
     CDS             complement(26743..28257)
                     /locus_tag="LK889_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBK5094935.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28395..29201
                     /locus_tag="LK889_000680"
     CDS             28395..29201
                     /locus_tag="LK889_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094936.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29376..29945)
                     /locus_tag="LK889_000681"
     CDS             complement(29376..29945)
                     /locus_tag="LK889_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094937.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30028..32088)
                     /locus_tag="LK889_000682"
     CDS             complement(30028..32088)
                     /locus_tag="LK889_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBK5094938.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32365..33843)
                     /locus_tag="LK889_000683"
     CDS             complement(32365..33843)
                     /locus_tag="LK889_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5094939.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34141..35076)
                     /gene="murB"
                     /locus_tag="LK889_000684"
     CDS             complement(34141..35076)
                     /gene="murB"
                     /locus_tag="LK889_000684"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK5094940.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35149..36069)
                     /locus_tag="LK889_000685"
     CDS             complement(35149..36069)
                     /locus_tag="LK889_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5094941.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36182..36934
                     /gene="xth"
                     /locus_tag="LK889_000686"
     CDS             36182..36934
                     /gene="xth"
                     /locus_tag="LK889_000686"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK5094942.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37798..38331
                     /locus_tag="LK889_000687"
     CDS             37798..38331
                     /locus_tag="LK889_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBK5094943.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(38821..39345)
                     /locus_tag="LK889_000688"
     CDS             complement(38821..39345)
                     /locus_tag="LK889_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094944.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(39338..39811)
                     /gene="tsaE"
                     /locus_tag="LK889_000689"
     CDS             complement(39338..39811)
                     /gene="tsaE"
                     /locus_tag="LK889_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK5094945.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(39934..40914)
                     /gene="pta"
                     /locus_tag="LK889_000690"
     CDS             complement(39934..40914)
                     /gene="pta"
                     /locus_tag="LK889_000690"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK5094946.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(41067..41747)
                     /locus_tag="LK889_000691"
     CDS             complement(41067..41747)
                     /locus_tag="LK889_000691"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK5094947.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            41873..42742
                     /locus_tag="LK889_000692"
     CDS             41873..42742
                     /locus_tag="LK889_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5094948.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            42768..43283
                     /locus_tag="LK889_000693"
     CDS             42768..43283
                     /locus_tag="LK889_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094949.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(43330..43923)
                     /locus_tag="LK889_000694"
     CDS             complement(43330..43923)
                     /locus_tag="LK889_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5094950.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(43948..44127)
                     /locus_tag="LK889_000695"
     CDS             complement(43948..44127)
                     /locus_tag="LK889_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094951.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(44204..44713)
                     /locus_tag="LK889_000696"
     CDS             complement(44204..44713)
                     /locus_tag="LK889_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094952.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(44809..45189)
                     /locus_tag="LK889_000697"
     CDS             complement(44809..45189)
                     /locus_tag="LK889_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5094953.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            45519..46268
                     /locus_tag="LK889_000698"
     CDS             45519..46268
                     /locus_tag="LK889_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBK5094954.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(46452..47729)
                     /locus_tag="LK889_000699"
     CDS             complement(46452..47729)
                     /locus_tag="LK889_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5094955.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(47808..48452)
                     /gene="pcp"
                     /locus_tag="LK889_000700"
     CDS             complement(47808..48452)
                     /gene="pcp"
                     /locus_tag="LK889_000700"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK5094956.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(48651..49595)
                     /locus_tag="LK889_000701"
     CDS             complement(48651..49595)
                     /locus_tag="LK889_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK5094957.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(49597..50283)
                     /locus_tag="LK889_000702"
     CDS             complement(49597..50283)
                     /locus_tag="LK889_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK5094958.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            50757..51077
                     /locus_tag="LK889_000703"
     CDS             50757..51077
                     /locus_tag="LK889_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK5094959.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            51231..52136
                     /locus_tag="LK889_000704"
     CDS             51231..52136
                     /locus_tag="LK889_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5094960.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            52133..52906
                     /locus_tag="LK889_000705"
     CDS             52133..52906
                     /locus_tag="LK889_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5094961.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(53087..54511)
                     /locus_tag="LK889_000706"
     CDS             complement(53087..54511)
                     /locus_tag="LK889_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5094962.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            54722..55168
                     /gene="nrdI"
                     /locus_tag="LK889_000707"
     CDS             54722..55168
                     /gene="nrdI"
                     /locus_tag="LK889_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK5094963.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(55233..57215)
                     /locus_tag="LK889_000708"
     CDS             complement(55233..57215)
                     /locus_tag="LK889_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5094964.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(57229..58125)
                     /gene="murQ"
                     /locus_tag="LK889_000709"
     CDS             complement(57229..58125)
                     /gene="murQ"
                     /locus_tag="LK889_000709"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK5094965.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(58128..59189)
                     /locus_tag="LK889_000710"
     CDS             complement(58128..59189)
                     /locus_tag="LK889_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5094966.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(59400..59966)
                     /locus_tag="LK889_000711"
     CDS             complement(59400..59966)
                     /locus_tag="LK889_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5094967.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(59994..61496)
                     /gene="eat(A)"
                     /locus_tag="LK889_000712"
     CDS             complement(59994..61496)
                     /gene="eat(A)"
                     /locus_tag="LK889_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK5094968.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(61754..62875)
                     /locus_tag="LK889_000713"
     CDS             complement(61754..62875)
                     /locus_tag="LK889_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK5094969.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            63001..63942
                     /locus_tag="LK889_000714"
     CDS             63001..63942
                     /locus_tag="LK889_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK5094970.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(64092..64988)
                     /locus_tag="LK889_000715"
     CDS             complement(64092..64988)
                     /locus_tag="LK889_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK5094971.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            65257..66597
                     /locus_tag="LK889_000716"
     CDS             65257..66597
                     /locus_tag="LK889_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5094972.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            66815..67258
                     /locus_tag="LK889_000717"
     CDS             66815..67258
                     /locus_tag="LK889_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK5094973.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT        19976 a        13995 c        11788 g        22003 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagctgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactagc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
    26521 cccacgctct ttttccgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca
    26581 accaagttaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
    26641 cagctttttc acaacctctt cactaaacag tgttcaatca gctgaccctc ggtattagct
    26701 caaaacgctg atttcacaac ctctttttat ttacgttcta tttcattcat tttccgaagc
    26761 aaatcgagct gcttgtatgc ccgcttgtcg cccaaaaata atgatctccg caactgaatt
    26821 tccaccgatc cggttgtttc catgtaagcc acctgtgact tcaccagcag catataaccc
    26881 cctaattggc tggttttctt tatctaatac ttcagtgttc gtattgattt tcacaccgcc
    26941 catcgtgtaa tggattcctg gtgcgatttt gatcgcataa taattcggag acgataagtc
    27001 atgttccatg gctgtggcac ggttgaattg agcatctttt ttctccgcaa catccgtatt
    27061 ccatccatca actgtttttg ctagtgtttc ttttggtaaa tcgatttttt ctgctaattc
    27121 ttccattgtc tttccagttg tgacatatcc tttttggtca taaaattcaa tagctgtggc
    27181 acgatcacgc acgccttggt caaaaatcaa ataagctgat ttttcaggca atgctgtaat
    27241 tgctgcagat actttatccc ttgtatccat ttcattgaca aatcttttcc cctcttgatc
    27301 gaccaagatt gctccttctc cgcgaacaac ctcaccaatc aagaccccct catcctgctg
    27361 cacagttgga tgaatttgta ttttatccat atcaacggtt tgtccaccta atttttcaat
    27421 catggtgata ccatctccag ttgatccttc ttgatttgtc gtaatataat cagctaaatc
    27481 gggacgatat ttttcaataa attctttgct ggcaccaaaa ccgcctgtgg taactacgat
    27541 tgcttttccg gtgacctctt ttggttcttc tccttgaaca tgtacagtca cttcattgac
    27601 cgtttcccca tttttcttaa tgtctgtcac atctgcgttg acaaaaatag ggatttcccg
    27661 ctcatggacg ttattcaata acccctccac taagtatcca ccaatagccg agccatcaga
    27721 cggacgatgt gtcctctttt cactcatccc tccagtgatc gttaagttgt ccagtttgat
    27781 accgttttga tctagccaat cgaccgcatc agcagagtga tcgacaaagt atcgaagcat
    27841 ttctttatca ttcgttcctc caccaccttt tagcgtttct tcatagaagg catcattcga
    27901 gtctttgatt ccttcttctt tttgaaattt tgtttctgaa gcattcatac cgcttgatga
    27961 tttagatgtg tttcctccgg caacaggcat cttttccaaa attactggat tcaacccagc
    28021 atcttttgct tcgatagctg ctgccatccc tgcaccacca gctccaataa tcacgacatc
    28081 ataagattct ttcatttctt ttggatctgt atatacttgt tcagaagcgc ctgatgatac
    28141 ttcggcagat tccttcgtac tagaacttgt ctgcgctgta tttttctgat ctgcgccgca
    28201 tgcgctcata gcaagaagtg caacggctgt taataaacca gcaactaatt ttttcatttg
    28261 tatccccctt ttctccttat actatattaa gtgatttttt tcacaatatc aactctgttt
    28321 ttctttaagt catatagctt tggtaaacac acgcttctta gtataattaa ggatatacat
    28381 gaaggagtta ttttatgaat catttgaaaa ccttttttac gaacagtaca aaaactgcag
    28441 gtagaatcac aaacggaatc tatattttat tacgtatcat cgtcgttatt atgctagtgg
    28501 atcaggtctg gaaccaacac tggggaaata caatgctttt attactgaca ttaattttat
    28561 ttgaaatccc ttattttctg gaaaagatat taaagataca gattccaaat atgctagaaa
    28621 tcattttgtt atcttttatt tttagttcaa cagttttagg agagttaagt gatttttatg
    28681 gctatttttc aatgtgggat acttttctac atgcattcag cggattttta gctgggggtg
    28741 tcggcttttc ccttgtttat ttgctcaata aaaatacaaa aaccatgaac ttgactcctt
    28801 tattactagc agttgtttcc ttctgttttt cgatgacagt tggtgttatg tgggagtttg
    28861 tggaattttc agcagataaa ttactgcaat tcgatatgca aaaagatacg atcatccagc
    28921 aaatcaatag tgtggagtta agtaaagacg gaaacactgt ctatcatgtt ccagatatca
    28981 ctcggacaat cattgaaagt caaaatgcag atggtaaaac agataaaaca atcatccaaa
    29041 atggttactt agacgtggga atcatggata ctatgaagga tttatttgtt aattttattg
    29101 gagcagttgt tttcagtgta tttggttacc tctatagtcg aaacgattac caaaaattta
    29161 cgttcatcaa gaattttatt cctaaaaaaa tcaaacgata aaacatgcta aaaaacaaga
    29221 gaaggggcag tgacaatact tttgtcactg ccccttcttt tttgaatgta tggcatgcca
    29281 ttagttatcc gatagtatta actcaaaaaa gtaagatatg aggaatgact atttcgtttt
    29341 ttatcccaat actgaggact tatttcacca tttcgttatt tatctaattt acttaaaacc
    29401 tgcataactt tttcataatc tggattaggt gtgccgtcta gattttcagc aataaaagta
    29461 aaacccattt ttttataaag agcaatcgcc tgatggctcc atgtctgtgt gtgaagatag
    29521 attggtccgt ccgtatggag ggtaggaaat tcttgcaaga ctctggcagt caatgctttt
    29581 gcgagccctt ttccttggta atctggtttc actgctaacc aatgaaaaac cggatacact
    29641 tgctctcttg atttttttcg ccaagcggta caggttgcta ctttttctcc tttttttgtc
    29701 tctacaaaaa acatccttct ttcaagttct tcaggataag gagcaaagtt ttgttgaaaa
    29761 tactcaagtg cttcatctgt cgtttgaaat tccccaacgg aagtctcaat ctccgcccaa
    29821 gcagattcat cttctttttg ataaaatcga aattcaaaac cttccgctaa aggcgtgatt
    29881 ggaacatttt gttgaagtgg tcttgtcatc cagatttctg cgtaaggtaa cgttttatct
    29941 aacatttttc tatccctcct cgtctccatt atgccaaata acatatgact cagctactta
    30001 tttgactata acagatagag atataaatca gaaccagtca tctgcgtcaa attcatttcg
    30061 attagcgtat acccatgttc ctgccatacc tcgcatgaaa tctcctaaag cagaagaagg
    30121 aatacctcca ccaggtgcgg gtgttcccgg actaggaagt ggtacttgcg gctgcagatg
    30181 ctcatcggtg gaatcaatag ttgacaagcg ttttttcttc gtttccttct ggtcatctga
    30241 ttccttctga tcttccgact gtttagcaac aaattctttt tctgattgtt gttttatttt
    30301 aggctctaaa atttcatttt ccaatccaga aacttgtggg gccaaatgag cttcttgcct
    30361 tatcgtttta ttagattctg agatttttct agatattctt gctaactcta ggcttcttgt
    30421 ttgttttact ttccctgttg gagaaactgc atgagccatt tgttgttttt tttctgacaa
    30481 gctttcttct tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg
    30541 taatagtgac tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat
    30601 cgtaaaatca cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt
    30661 aaccgataca tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa
    30721 ctcttctttg tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg
    30781 attgtctggt atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt
    30841 catcagttct gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc
    30901 gtttgcttgc atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa
    30961 atctatattt ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt
    31021 tacactactc atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg
    31081 agtgatgaca gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc
    31141 cacatttgat gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt
    31201 agtatctact gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt
    31261 tgttacatat tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc
    31321 ttttcctttg atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt
    31381 ttttaaagca attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt
    31441 atccacgata ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc
    31501 gttcttcagc ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat
    31561 tttctttctt tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc
    31621 accgtccttt atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc
    31681 accttttgta ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc
    31741 cgatgtccat aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact
    31801 gactttatta taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt
    31861 atgagtaata gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat
    31921 ggtttgtgtc gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag
    31981 catcgtgcgc atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa
    32041 acgacaagcg tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc
    32101 ctccctactc ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa
    32161 taaaatcaaa agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa
    32221 gaaaatcgga ataataactt atgtactttt taattttgtt aagtaatata tctagttata
    32281 tctagttata tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg
    32341 agacaaagct gccatccata aatactagct gattttttca gttaaatgtc tatttttttc
    32401 tctgatgatg gtatttccaa aatattccca gtcatctgga gtcggtggat ttttccaaat
    32461 tatctgttct ttattaaatt tttcaagtaa acaatcagat aaataaatat tcgtacgaga
    32521 agtgattcga ctctcaatca caatattgta atttttaaat cctttcactt gagaaataat
    32581 gtaatcggta taagcagatc cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa
    32641 atatgatacc ggacaaatac cgaccaataa aaagagataa tcataaaata agcgaatcat
    32701 gaaacgttca tcttttccca agaaatagcg tttattctct tgtatgacct tttggattgc
    32761 tgtactgaat attggatacg tctcgaagaa aaactgtagc tgctttttag acgtaaagct
    32821 agtcatctca aagtcgaata tccaattttt tcgattgaca tgcatcatac ccatacgaag
    32881 ttcttttcgt atatatagtc tttcttcttc agagatttca atcgaaaatg gtaattgatc
    32941 aatcaattcc actgacattt tttgactaat catgtggata tcatgaaaat gagcttccgc
    33001 aagtccaaat gcgccatcta attcaaattc ttcttccgtg atcaaaaaca acatgatata
    33061 agaaatctca tcaatgatct gctctttagg gatatgaaga aaagaaatat gtgataaatc
    33121 cttgatgatc ttcattcctt ctgagctctt aaagtattca tctgaaaaga aacgttttcc
    33181 gattggttga ttcccatagg ccctcaacaa actaatagtg gttagcaaat tgaatttgat
    33241 tcgttttgtt gcagacagtt ttaaattgaa cagatattca aaatattgca cgatactctt
    33301 tgcttcattc atgacgcttt ctctaaatgg catagaaaaa ccattaaaga ccccgaaata
    33361 taccccaaat aatacgttcc gtatgtttat ttcttcccca actaatatgt ttttctttaa
    33421 ctttatttga tgctgttcaa gaaaagagac taattctctt ctttttacat aggctcttga
    33481 tatgcttaaa aaatgattct ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc
    33541 cgttaacagt cgagcaattt ttgatcgttg aaagtagatt cttcttaaat ttttgatatc
    33601 cgtcaaattc agacgttcga catggatctc tgcttcttct gaaatttgga tctctggctt
    33661 tgttttgaat tcagtaaatt ctttatttaa ttctatgata taatttcttg ttttaaattt
    33721 agacaaacct aatgtttcat ataacttagg cagcactaca taattctttt tctcaaattc
    33781 taaatatcta gctagcagca gtttttctct atcttcctct tctataaggt aatccaactt
    33841 catatcctac acctcaaaaa atgcctcata ccaattactt gatatgcaga tgaaagttca
    33901 tcttttcacc tatactgatt ttatgatgga ttatgcttgt tttttcatca gttattctca
    33961 ttttttatct tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct
    34021 atattttttg ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca
    34081 caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta
    34141 ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac
    34201 ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa
    34261 ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc
    34321 agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc
    34381 tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg
    34441 ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc
    34501 tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg
    34561 acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa
    34621 aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata
    34681 aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc
    34741 tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt
    34801 tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc
    34861 atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg
    34921 gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc
    34981 tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat
    35041 cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca
    35101 aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg
    35161 acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg
    35221 tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat
    35281 tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt
    35341 tccatcagca tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt
    35401 acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc
    35461 cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct
    35521 tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag
    35581 atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt
    35641 ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa
    35701 gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc
    35761 acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc
    35821 tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc
    35881 gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt
    35941 ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg
    36001 ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc
    36061 aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac cattttttac
    36121 taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt
    36181 attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt
    36241 agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc
    36301 aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa
    36361 aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat
    36421 gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt
    36481 tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt
    36541 agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat
    36601 cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa
    36661 tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa
    36721 cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg
    36781 gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac
    36841 gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag
    36901 tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt
    36961 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata
    37021 ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc
    37081 atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct
    37141 tattaccgca gatacaagct tttgaacacc gtttattaag aggttgtgaa aaagctgcac
    37201 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg
    37261 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg
    37321 cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt
    37381 tggacttatt accgcagatg caagcttttg aacaccgttt attgagaagt tgtgaaatca
    37441 gcgtcctgac ctgagtatta gagaaacaaa tggcaaaata gttcctcata ttttgctatt
    37501 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa
    37561 agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac aactttgcat
    37621 aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca caaggaatga
    37681 gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt ttttgttaaa
    37741 tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt tacagaaatg
    37801 aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc ttgttctctt
    37861 gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt gatacagcct
    37921 gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga agacgtacgc
    37981 aatgcgccga aattcccaga agtctggcaa actatccaac catattttca aaaaaatcgt
    38041 ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg cttagattat
    38101 tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg
    38161 tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa catccaatta
    38221 gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa
    38281 gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta aaagaatact
    38341 tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt
    38401 tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg agctggcagg
    38461 agaacaccgt ttattggaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    38521 ccaaacaaaa atagcttctc atatttttca tttggttgga cttattaccg cagatgcaag
    38581 cttttggaca ccgtttacca agaggttgtg aaatcagcgt tttgacctga gtattagaga
    38641 aacaaacggc aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
    38701 ctgattgaac accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc
    38761 ttcgtgtgtt tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt
    38821 ctattcaatc aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc
    38881 ccctctaggc atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct
    38941 ttcattacga acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca
    39001 actgatgatt tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat
    39061 actgataccg acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc
    39121 tgttccaata atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta
    39181 tatataatcc aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa
    39241 aactaaaaat tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc
    39301 tcctggcact gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt
    39361 tttctagaat tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat
    39421 accgcttttc taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca
    39481 gtgcttcttc taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt
    39541 catctaaccc taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg
    39601 gaatccgacc ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg
    39661 ttatccctaa tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac
    39721 cagtcaatat cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt
    39781 ctgtcattgt taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt
    39841 ttgccagtat atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc
    39901 agctgctgtt tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt
    39961 gataatcgat aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg
    40021 tttgttcaag ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat
    40081 tttatagccg atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc
    40141 tacttctgaa tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg
    40201 caattcgccg tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac
    40261 tactttgtct acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac
    40321 ttttggttcg atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa
    40381 ttcttgcgca gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc
    40441 tcttccacgt accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat
    40501 gatctgaagt gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc
    40561 gtctgcaatc cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt
    40621 ttctgcttgt tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc
    40681 atcccactta tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct
    40741 ggcatgcgct gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc
    40801 ttctgctttc aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac
    40861 gattttgatc ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg
    40921 acctccagac gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat
    40981 tttatctgta tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg
    41041 cactataaca ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt
    41101 tcttctccta acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag
    41161 ccacgacttg cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta
    41221 tcgatcattt tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt
    41281 tcccgttcat ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga
    41341 tgagaatacg cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt
    41401 ttagcccatg acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc
    41461 tctttataga tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat
    41521 aagccatgag cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact
    41581 tcttcataag gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt
    41641 tgggacgcat attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat
    41701 tctgaggaca aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc
    41761 taataaaaag aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac
    41821 ttgattaata tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa
    41881 attaattgca tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga
    41941 aaatgccgag gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg
    42001 tcgaaactat caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat
    42061 cactttgaac ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat
    42121 tccaaataat caagcaatct ctgttttaga tattctagat gctaacggta tttactatga
    42181 agtagcaacc aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc
    42241 ttccatggtg gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc
    42301 agctaagctg gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga
    42361 aaagaatctg gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg
    42421 tccagttgga aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa
    42481 caatatcgaa gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca
    42541 cgagcgtggg atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag
    42601 catgctgcaa gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga
    42661 ttatgccaaa tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg
    42721 cgcgattgca gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt
    42781 tcattcagga aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac
    42841 attccctgtt tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg
    42901 ccatgaatgg cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt
    42961 ttgagctgta taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg
    43021 gtgactttta ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc
    43081 aatatcaaat ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt
    43141 caggagatta ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta
    43201 ttgctgcggt tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt
    43261 cttaccgact taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg
    43321 tacatctttt tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg
    43381 accgacaact ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc
    43441 cacacttaga ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa
    43501 taatcgcgaa ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata
    43561 ccattcgttg ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata
    43621 tttctctaag ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc
    43681 gtaagcaact cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta
    43741 acgataattt ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt
    43801 ctgcttcaaa aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc
    43861 tggagcatct tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa
    43921 catgcagaaa aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat
    43981 gaaataacca tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg
    44041 catttcttta tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc
    44101 gcaattaggg catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt
    44161 agcttactct tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata
    44221 cctgacggtc ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga
    44281 ctagaagtgt ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca
    44341 tcgctgcttg cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg
    44401 ccttttccgt gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt
    44461 gtccttcaca aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt
    44521 caactgcaaa atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg
    44581 ctgctttttt caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg
    44641 cattgagcag tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca
    44701 tcattttttt cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac
    44761 gaatgaaaaa tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag
    44821 ttctaatggc aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc
    44881 ttctctaatc ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat
    44941 atcgtctact ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg
    45001 tggcgcatta gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt
    45061 atagtcatct cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac
    45121 ataaaaatca cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt
    45181 aaggttcata tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt
    45241 ccattgtaaa tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg
    45301 caaaaaaaac agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga
    45361 aagtaagaaa ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta
    45421 taattgaaag actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca
    45481 gaacacacta catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt
    45541 tagctcatca gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg
    45601 gctgccaggt catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta
    45661 tcggtaccac gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg
    45721 gtattttagg tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact
    45781 atttaagtat cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa
    45841 gtaaagaatg gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt
    45901 ttatcgcaat catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt
    45961 atacagatga tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg
    46021 gtatcatcag tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg
    46081 ctcaaatggt cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg
    46141 tgaacagctc tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata
    46201 accaaaaaga acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac
    46261 acgcataaaa aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    46321 cacgtataaa cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    46381 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    46441 gcctctattt tttatagtaa ttccttcaga tactttcttg cctcatcatt gaaacgcagt
    46501 gattcttcta acttctgata atattccaac gcttgtttct gagcttcagt aaaacgataa
    46561 tgttttcctt tccatttgtc gattgtctct tgcacatttc cagattggat atctttcact
    46621 actagttgcc atttttcttt tgagactgta cggtctttct tatcagcaga ttgtatagct
    46681 tctcgttctt tacgcaatac ttttaaaaaa ccatttctga ctatccgagg aatgctgtcg
    46741 aagaaccctt ctgtaaaacc atagtgacaa tattgcttcc aaataaatag atagattttt
    46801 ggttggatga atcgttggtt tttcgcaaaa tattccatct gagcagtgac ttctggtgtt
    46861 cgttgacgtt ttgtctttct caacacggaa ataaatgttt caaaataagc attaggatgt
    46921 actggcactt cttcatatac gatggattgc caatctttac gtaaaaatgc gcgatagttg
    46981 atcaagaatt cttctacatc ctttactaat tcttctgcaa agtcgtcaaa tagttctttt
    47041 tttatctgtc gcttttttga ctgcccattg atgtgttcac ttattttctt gatttcattt
    47101 tccaatgctt gataatccgc tatgtaaatc ggcatttcta cttggcgaaa ttctgcgagc
    47161 cgctgagcta attttatatc cggtaaatat ggctgtttca tccgacgatt ttggtacaag
    47221 acaaaacagt atttttgcca tagtccttca ggaaaatcga tattcttctg cttaagccat
    47281 aataattcgt ctaaggccag atcaactttt gtcattccct gatggatcga agctgccact
    47341 gtcggagaga catgcaggtg atcggcaaag tggttgatgc ctagttttag gatctttccg
    47401 gtttttggcg atttaacgat gtattgcact tttaattctc taccacactc acaatacaat
    47461 ttttgctggc ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga aaacacccaa
    47521 tctgttgcag ctaaataatg ttcttccata aaacgagagc tgattagata tcggcggtgc
    47581 ttatcgacta gttcccgctg ttttttcgtt aattttgcat agatctccca acgttgactg
    47641 cttgtgtatt gtttagaaat tttgatggga tagcttttgc tgatttcttt tggaaaccat
    47701 tcttcaaaga cggattgcca ttcttccacg gctgtttctt tcctttctaa ttacaaaaag
    47761 ctggctacta acataacgtt agcaaccagc cagtgtttct tgctaaatta gtgagttgtt
    47821 ccggcatttc cggaaatatc ttctttattt tctactgctg cttcaactgc ataagtcaaa
    47881 gaagaagcaa tgtcttctaa agacatagaa ggcgtaccca ctggacgatc gatcacttga
    47941 tcgggtgaga atggcacgtg gatgaaccca cctcgaattg ccgggaattg tgtttcgatc
    48001 aaatgaagca aacgatacat gatatcattg cagacaaatg ttcctgctgt ataagagatg
    48061 tacgctggta gaccatgatc ttgtacattt tttgtcattg ctttgattgg caatgaggta
    48121 aagtaagcgg ctggaccgtt ttcttctaaa gccgtatcga atggctgatt tccttcatta
    48181 tcagggattc ttgcttctgc tagattgatt gcgacacgtt cgaacgagac tgctgaccgc
    48241 ccacctgctt gtccgacaca gatcacgaca tctggttggt gctgttggat ggcttctttg
    48301 actacttgac tgcttttttc gaatacggtc ggtacttcca ctttgatgat ttcagcaccg
    48361 ctgatttcat ctggcatttt ttttactgct tcataggctg gattcacttt gtccccacca
    48421 aatggatcaa aacctgttac tagtactttc atctgttgtt cctccgtccg attgatttaa
    48481 catatggtta tcttttatca taccattttc cttgaacagc gtaaataaag gaaacaatag
    48541 tttaaataac ataacagatc aaacttaata tattcttata aaaaaatagt gttccactac
    48601 aatgttagaa gaggttgcga caattattct gtcacaaccc ctctcttcta ttatttaaat
    48661 agaatcatat aagcaatttg gaaaactagc ataaataacg caataaacaa ctggtttttg
    48721 atcactccgt atttgtcttt catctccagc atcgctacag gcacgatatt gaagttagca
    48781 gccatcggtg tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat tcctattaac
    48841 gctggattcg ctccatgact aaatacgaat ggtgcaccga tcccaaccgt catcacggtg
    48901 atggccgcaa atgcattccc catgattacc gtaaatatca ccatgccaag agcgtaaatg
    48961 atgatgccga tgttcacatt tccttctgga acgatcgtac ctactgcatt ggaaatcact
    49021 gtcccaactc cagcgcttgt aaataccgca cctagagaag ctaaaagcat tggcaacatg
    49081 cttaacggcc ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt acttggtcga
    49141 ttatctcgag aaaagaacat caagattagc atggctgcaa aaacaccgac gcccacgccg
    49201 actaaagcac ctaaattggt aaatagagca aacaagatcg caaagacacc gatactcaaa
    49261 gctggaataa aaattttcat tcctagtttg tctgacattt tttccatata ttcttttgat
    49321 ggaagccggc tttctccttt gctgactttc ttagcaattg ctggtatggt catcgcaaag
    49381 atcaagccac cgttcacgat tgccggaagc catcttcctc ctgcaataac tacccctaag
    49441 accgtccaga aaaaggctga cccataacga tgaggattgg ttttatctgt taaatttctt
    49501 actccagtgt aaatcaatac gagtcccatc acgatataaa ggatctccag taatttatcg
    49561 cctaataaaa tatcaggatt tgtaaaaaag tccatcttat tgcccctttc ctggataata
    49621 tttcttcgct aatttctttt ctttaagata gaagtagaca cttgccacaa ctaaagccgt
    49681 gatagccact ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat atcctagatc
    49741 tttcagtgtt gattggacta gcaacgcccc tgctcctccg atgaatagta cttggccaaa
    49801 gaaccagcag acattttcca tagctgaagc catacctttt agttcttctt catgcttagg
    49861 atggacttct aacccttttg attcgatggt ccccaatgcc atcggtaaaa tgattggacg
    49921 aacaaatcca gcaactccac caaaactcac gttaaaagcc gcaaagatca ttcgaaagat
    49981 cccataaata tctacaatca taccagcaga tactttcttg aatcgcttga tcaaggaagc
    50041 cgctgattct ttcagaccat ttctttcaag cgttcctgtt gccaacataa tgatgataaa
    50101 aatcgccatc ccgcgattgt ttacaaaact tgtacctaaa agttctaaca ttccttgaat
    50161 gccaagaccg ccagtcaaag ctgtaacaac taaagcaacc attacgatca atatcgagtc
    50221 taatttaaat gcaaacccga gaataacaat caagacgcct aaaaggctaa gataattttc
    50281 catatatttt cctccataaa atgacaatat ttttttaatt ataaagcaaa acacataaaa
    50341 aatatagttg ttttctcacg aaatagacac tatttttaaa tatttttcgt tattttttta
    50401 tcacaaaaat tatcttactt attgtaaggt aaagcttttt ttaatttatt gcccgataat
    50461 atatagaaat aaggtctagt tactgttttt tttaaatgat taataaataa caacttcttt
    50521 ctatgaaatt gcaaagcatt catcttcttt tccgctaacg atatatccgt cagtgcttgg
    50581 tacactttac taaaaagtat gagattgccg atatctggat aggtgtgcta tactacagaa
    50641 aaagatatac aatctgccac cgggtaggat aaagaggatg atcattccat taggtctttg
    50701 aagggatgat tttcctcttt ttgtgtattt ttttatagtt aaggaggaat gtaagtatgg
    50761 catggatgga attaatcata gcaggaattt tggaagtatt ctggtcaacg atgatgaaat
    50821 ggagtgatgg tttttctaaa atcaattatt ctttttacac agtgattggc atgattgcta
    50881 gtttttactt tttgtcaaag gcaatcaaaa gtttgccgat gagtctctct tatcctatct
    50941 ggacagggat tggcgcagtt ggttcggtca taattggtgt ggtcttgttt catgataagc
    51001 tgaatctttc cacttggttc tttgtgggga tgctactcat cagtatcatt ggcatcaaga
    51061 ttactagcgg ccactagaca cttggaataa gtactgatca gaaaaaataa gcctttttat
    51121 ttaatattca atttttttat gaaaaaataa gaaataagtc caatgaccta gcgacttctc
    51181 cactcatcag gtgtatgctt gtactatcta tagaaaagga gtgaatattt ttgaaaagtc
    51241 tttcggtcca agaattatcc aagaactttg gtcacaaaca agccctcagc gatgtcagtt
    51301 ttagcttatc ccctggcaaa atcgttggat tagtaggtcc taatggcgct ggaaaaacaa
    51361 cgatcatgaa agctatttta ggactgatcc attactcaga tggaacaatc tgtattggtg
    51421 aaaatgagat ttctccctct agtcataaag ggttggaaca agtaggagca ttgatcgaat
    51481 acccgggtat ctatccattc ttaacaggct atgatcatct gaagttattc tctgaaacca
    51541 acgatgtatc tgctatcgac actatcgtga atcagctcaa gatggaaaag tacattcaca
    51601 aaaaagcgaa atcctactcg ttgggaatga aacaaaaatt gggaattgct cttgccttac
    51661 tgaaccatcc gtctatcgtt attttagatg agccgatgaa cggtttggat ccacaagcaa
    51721 caagagatgt gcgaaaaatc attaccgact tggctactca gggcacctct ttcttgatct
    51781 ccagtcactt actcagcgag cttgaaaaaa tggtcgatga gctgatcctg atcaatcaag
    51841 gaaagatcat caaacaatgt acaatggctg aattgaacca aacagcgcaa gatttcatcg
    51901 ttatctctac aacaaatgat ctagctgctc gaacgatctt gacagaagct ggatatactt
    51961 tggaagatcc tgataaaatc aagcttttaa aagaaaacga agaattatta ggaaccgtga
    52021 tccagctatt gtctgcacaa aatatccatg tgacagacgt ccagcatctg acgaatgatt
    52081 tggaatcttc tctattggaa attctggatt cagtaaaggg ggaagaagaa aaatgacatt
    52141 gcttgtaaaa caagaattat ttaaattgat caagaaaaag tcaactgctg ttttatctgt
    52201 tctattagtt gttttgctca taggaacagc acttctagcc aaaaaataca cgacgatcat
    52261 tgatccagtt gaaatgactg ctcagctttt tagtgccaca tcttggattg tctttatcat
    52321 gattgcagct gcaagcacga tcatttcgat ggaagcacaa tatggtacat tgaaaaatct
    52381 cctttatcga aagtattctc gaggagaaat attagtaagc aaatggatca cattagttat
    52441 ttattctgtt tatttatacc ttttagctat tattgtcact gtgttgatga aactgattct
    52501 tttcccttcg atttccttca cggagaaggt ttctactggt caaacactga tccaaagctt
    52561 gttcacttat actttgggta gttatatcgg tttatggctg attttaagtt tagtgttgat
    52621 gattgcttgc tttatcaata gttccggcgc atccatctca gcaggaatcg tcttttattt
    52681 tgcttcgtca atcatatctg gtatccttat tgcactcatc caaaaatggg agtggatcaa
    52741 atggaatccg atcagtatgt tgaatctgca aaaccaaatt ggaaacgaag agatcatgaa
    52801 gcaattgact catctttcta ctaatcaaat gctattcggt aatttagctt acatcgtttt
    52861 gttcttagca ttaggatatc tcgtatttaa gcgaaaaaat gtatgatgac tcatcaaaaa
    52921 agaggctgtg aaagagacgt tcagccttat tgccgattgg ctgtctctta aacaccgttt
    52981 attctgaaaa aggagctgtg acaacatttg tgtcacagct cctttcctag tatgtttttg
    53041 aataaacgac aggctcacaa cctctcttta tacttctttt atacttctat acgtgaagca
    53101 tcccagtggc aaataaaacc attgcaatca ctccgaccat cgtcacacct ataagtaaaa
    53161 tcttatctaa gtggatatgt tgtacaggct cgttttgctc cttacgtgct ttgaggtaaa
    53221 aataaatgcc tggcaagaaa agaacaagaa caagcattac ttcattaaag gctacgacta
    53281 gcatagccca tacttgaaag gcacttgcta aaagccctac taaaaattgg acccattctt
    53341 tccgctgata agaaagtttc atttggtaca atcctaagaa aagccacgtg aagaaaatag
    53401 ctgctgtacc aagtgtgtaa gcaaagttat aagcactgtc agtgaataat aatgaaagta
    53461 aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc ccatttttgt
    53521 tgagttttcc ccatgcttta ggcaaaagtc cgtcttcagc catgtttccc attgtttcaa
    53581 tcggtaagat cgtccatgag agccaaacac caactaaaga aatgatcaaa ccgccgttga
    53641 tcacgactgc tccccattta ccaaccatca tttcaaacac atagcccata gcaggctgcg
    53701 ccttgatcgt ttgcagctga ttcatcgtca ttgtcccata tggaagcatg gaaaccaaga
    53761 cgtaaataat aatcaagccg aatatcccta gaatcgttgc tttcccaaca tctgattgtt
    53821 tttttgctcg tgaacctacg acagaggctc cttcaatacc aacaaatacc caaagcatca
    53881 ccatgatgca actcaatact tgactggaaa acgggatcgg ctgtccattc gcattcaata
    53941 taatgtcgct agaaaagtta tcaatgaatt gttggatcga aaacgtgctg atagctacta
    54001 caataaataa aaataatggc actaatttac agaatgtgac gatagcattg acgaaactag
    54061 cattttcgac tccccgattc actagccatg catatcccca taaaaagact gtactcatca
    54121 cgatcgatgc cagattttgc ccaccttcaa atactgggaa gaaatatcct aatgcgctca
    54181 tcaacaatgt tgcaaaagct acgttgccta accagcatgt gagccagtaa ccccaaccgc
    54241 tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc ccgcctttta
    54301 aatctggacg ttttttattg atattattga tcgacaagga gagcatcaac attgctgttc
    54361 ctacgagtac ccaactgaaa atagctgggc cgccagccga tccggagatg tccgtcataa
    54421 taccaaatac gccacctcca atcgctgaac caattactaa ggcagcaagt ggaattaaac
    54481 caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaaata aaaccgcttt
    54541 tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac tttaatagaa
    54601 atataattct accggctttt ttctcattat acaagcaaag cgcctagtcc ctttccaaat
    54661 tacaccacca aaaaacttaa aaaagatgct atactaaaag gaaagaattg gaggatgatc
    54721 catgaacatt ttatatatat cgatttcagg aaatacgcgt gcctttgcca aacatctagc
    54781 agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta ctttaaaaga
    54841 aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt tcgttcctac
    54901 ttatttagac ggcggaaatg gtttagataa cggtgataca gaaatcttga ccgaaacgat
    54961 gcgtgaatat ctggaacatg aagacaatca taaactttgt cttggtgtag tcggtagcgg
    55021 aaataaaaac ttcaataatc agtattgtct aacagccaaa caatatgcac aaacatttgg
    55081 ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga cacgaattta
    55141 tcagatttta gcagaaaaca ataaataata aaaaagaaga agctgaaaaa tagatttcag
    55201 tttcttcttt cgtttctatt gtttctatca tattaagctt tgacatccat cacaggtgct
    55261 tttgcttttg ctttacctgt tttttccaag acaaagtttt ttacacgatc catattggtt
    55321 accaagacga tcatggatgt ttctttgcct gctgctttga tcgcttctaa gtcagcttct
    55381 gctacaagcg tatcaggtgt cacttgctgc ccttctgtta ctttcacatc aaatggcgta
    55441 ccgcctaagt caactgtgtt gattcccata tgaagcaaga tttctaaacc atttggcgtt
    55501 gtgatcccaa tagcgtgttt cgttgggaag acagagctga ctaccccagt aactggagaa
    55561 tagatatgtc cgttttctgg ttctacaaag tagccttcac ccatcatctt ttgtgcaaaa
    55621 actggatcac ttgctttatc taaagaaaca actgttccgt cagcaactga tgagaggata
    55681 gtcacacttt cagctgaagt ttgaggtact actttttcag gcacttcatt tactgtggct
    55741 gtttcagtcg ctaattcttc tttttctaaa cggtctttag gaataccgaa gaagaatgta
    55801 gcaacgaagc caccagcata agctgccaat aatccaagga tgtagcctaa ccatttaccg
    55861 tcagagatca atgggatcaa agcggctcct gaaggtccaa tagcgatagc cccgacattt
    55921 ccgatcattc cgatcactgc cccgccgata cctccaccga tacaagcagt aataaatggg
    55981 cgtcctagag gtaatgttac tccgtaaatc aatggttcgc cgattcctag gatcccgact
    56041 ggtaaagctc ctttgatcat ttctgttaat tctttatctt ttttgcagcg aatccataat
    56101 gccaatgctg cacccacttg tcctgcacca gccatcgcaa gaattggaag caatagcgta
    56161 cgaccagttt catcaatcat ttgcacatgg attggagtca aaatttgatg cagtccgaac
    56221 ataaccatcg gcaagaataa tgtgcctaga acgaagccgg caaatgctcc accaacgcca
    56281 agtacccagt tgatcccacc gaccatgcca tcagaaatga aacctgctaa aggcatgatg
    56341 aagaaaattt caatcagacc gatggcaatc aacgaaagag ttggtgtaac gatgatatct
    56401 accgcatcag gtactacttt atgcagtttt ttctcaacga ttgacaatag ccaaacagcg
    56461 aagatgacac ctaggatccc accttgtcca gctgcaagag cttcaccagt aaataagttt
    56521 ttcagtggtg cttccggatt catccctgtc agcatcacaa cggccccgat aactccacca
    56581 agagttggtg ttgcaccaaa aacttgtgca gcattgatac ctgtatagat aactaggtag
    56641 ctaaacatcc cgtttttcaa gatgttcatc acatcgatat attgttgcca tgttgctgca
    56701 tcaagtgttc cggctgtgac taagtttgct aaaactgcgg caatccctgc cacgatccca
    56761 gtaccgacaa atgcaggaat cattggcaca aagatattag aaatgtcttt taataccgat
    56821 ttgattttag atggtttttg tttcgatttt tgtgccgctt tcatttcttg tgcccgttcg
    56881 tttacacggt cttttccaga tgctgatgaa tttgctgtag ctgtacttac agaagaaagt
    56941 ttttctccta atttcacgcc tgccaaatca accatttgct gtgctacttt gttcactttc
    57001 ccaggtccga tgatgacttg aagctgttca tcttctacta caccaagcac tcctggaatc
    57061 gcttttaatt cagagacatt tacaagatca tcattgatga tcttcatacg cacacgagtc
    57121 atgcagtgga caatgctttc tacattgccc atgccgcctg cccctttata aatttgtttt
    57181 gccagttctt ccaaagtcag ttcgtttttt gccatggtct tttccccctc ataaggtccc
    57241 ttttacaaat ccgtttgctt cagctaattt tgcttgagct tcctctttat cactatttgt
    57301 caaaatcatg acgatagcca gcttaacatt ttgatctgct tcttcaaagt acttttcagc
    57361 tgtttcgtaa tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt cgattagttt
    57421 ttcatttgtt ggtttgacat cgaccatcaa gttattgaat actttaccta taccaatcat
    57481 actgatagta gaaatcatat tcaagataag tttttgtgct gttcctgact tcaatcgagt
    57541 agagcctgtc aaaaattctg gacctgcatc gacttcgatt gggaaatccg cgtgtttact
    57601 gatttcggct tgtttattgc aagaaatcgt tcctgtgaca gcaccgatcg attttgcgta
    57661 atctaatcca ccgatcacat atggtgtacg accgctagct gcgataccaa taactatatc
    57721 attttttgtc aaattcagct tgattagatc ttcttgccct agctcataag aatcttcggc
    57781 cccttcgaca gctacagtca ttgcttcttc tccaccagca attagaccta tgaccatttc
    57841 cggttctaca ccgaatgtcg gtacgcattc agcagcatct aacacaccta gtcttccact
    57901 tgttccggct cccatataaa tcagacgtcc acctttttta aaggactcaa tcgtgttagc
    57961 aatcactggt tcgatacttg tcagttcttt ttcaaccgca gcggccactt tctgatcttc
    58021 tttgttcatt agttgaacgg cttgagaaat actcatctca tctaatccaa aagtttcttg
    58081 gtttctgcgt tccgttgtta ggttctctaa attgattttg ctcattttta tcttccttct
    58141 tttcttataa attgatagtt cataccagct ttgatttggt tgatcaatgg tagctcttct
    58201 ttgataactt tagctactac attcactttt tcatccgctg gcaaatcata tttggttaat
    58261 tggatctcac ccatatagcg caaatacttt tcgttatcta aagtaataga ccctttcata
    58321 cgcgtagctg tatttcttgc tgggataggc ggaatctctc gaaaacgtgc atctacgctt
    58381 cgtatgacat ctcttgcatc gtcttgccga ttcgtatgct tccctaatat atattcgtaa
    58441 aattcttcat ctacaggttc cgtatggagc aagactgtct gttctttttg gaaagaagca
    58501 aattgctcct gaacgttttc tgacagacct gcatctccga tgtaaacaat atccgtgtta
    58561 cttgctaata gatctagagc gcctgcaaga ggatggatcc ctcgatgttc ttccaaagtt
    58621 ggcaatcctt gatatagtgg gcctctcaac tctgtatctc ctggtacaaa accttggaca
    58681 aggagacctt gaattttgag ccattgattt tttttctgat accattcttt gtccagccct
    58741 gtttcgggac gtggataata attatgccaa gcttcaatct gtgagaaatc agcttctgct
    58801 tctttcaatt catcgatatc tttaggcgtg atcgtgctag cattcaaact gattttcaat
    58861 tgatgtgacc agtcggcaat ctgctgattg gaaatatgat aatccattct taaacctgtt
    58921 acaccaattg ctttcaaagg cgttagatct tctaatgaaa aacccgctcg ttccaatgcc
    58981 tctcctgaaa tatcgaccat caattctagt ttattctttt tcgcttcgct tcctagttcg
    59041 atcaatcgct tttgatatgc cgaaacatca tcttctggta tatgcatgga agtgaaaata
    59101 ccgacaaagc cattggatgc catttggcgg atatactgtt tcttctcttc tgacaaagaa
    59161 tcgttcataa atattgaaaa gccaaacata ttatccctct ttttctgtat ccgtcttcat
    59221 ttctatgact agagtatata tctaaagaaa caaaatttca atacttttat taaaataatt
    59281 aaatttcatt tctaaaggtt gagtttatat tatcaaaaga attgtcataa cctaaaaaag
    59341 gcactgctaa gcaaaagcag tgccttgaaa tattttattt aaataacaca aaaacataat
    59401 tactcataag aaaccaattc tgtattatct accatgaatg taaagaaccc ctcattaggc
    59461 atcggatgaa aataagcatt gatcactctt atcaaggcat tatggcaaac taacagatag
    59521 acattttctt cgtcttcgat gcattctttc aataaaggaa cgatccgtgc atacacatct
    59581 aagactgact ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt ttgaaagttc
    59641 tcatctttgc ttggcagtcc atcataatcc ccaaaatcca tttctaccaa tcgttcatca
    59701 acggtcagcg gcaagctgag acgatcagca actgcttgtg ctgtatctct tgctcgttga
    59761 agaggggaat ggatgatttt cgtgattgga acaggcaaat ctactacctt ttctgctagt
    59821 ttctgtgcct gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc gcaaactaat
    59881 ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg attctctcct
    59941 tcgttggcaa agttcccctt aaccatacct tgttgttctt gagattacaa gggttagatt
    60001 ctctcaagag aaataatctc agtagatact ttatccagga aggtttgatc atgttccact
    60061 agtaacatgg caggtttcac gttcaagatc agttgttcta attgttcttg attgaagaca
    60121 tccaaataat tcaatggttc atcccatgta tatagttcag ctggctgtga taaagattta
    60181 gccaattcca cttttttccg ttggcccata ctcatctgct cgatcttgtt atgaaaaaca
    60241 tctctttcca tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc tacttggttt
    60301 ttctctgcaa attccgccaa cgttcctcga ttgtcttcat aattttggct tgcataacta
    60361 atgctcagat gttttggctg gtatttttct cctataacct tgccgttgaa tgcccccaga
    60421 agataatgga taatggatga ctttcctgca ccgttaggac ctgaaatcgc cacccgctga
    60481 tgaggctcga ttgtaaaatg aattggctcg aataacagat tttcataccc taattgaaga
    60541 tcttctacgc ttaaaagtcg cttatggtga gacgcttggc tattcatcgt caaagaatcg
    60601 atatattcga tatcttttaa tagtttttct ttctcgctga tctgggtctc catccgattc
    60661 acgattgctt tggatcgttt catcgtccgt gcagcatcag cacccactgc tcctttattc
    60721 actcgtctag attcagtatc gatgaatccg acttgtttct ttgttttatc tccttctcgg
    60781 gaacgagacc attcggcttt ttcagctgct gttttcttta gcctactgac ttcttttttc
    60841 aatttttcat tttgagccat ttcaaattca tcacgaagtt ttttctgttc ttcatagata
    60901 gagaaattcc cttgataaag ttccagttga cttttttcga ttgctaaaac atggtccact
    60961 acttcatcga taaatccccg gtcatggctg accacgatga agccttgttt tttctttttc
    61021 aaataagccg ctacttgttt tctaccagag atatccaaat gattcgttgg ttcatcgatt
    61081 aacgggaaat gagtgtcatc cacaaataaa agtgccagta ggacttttgt cttctctccc
    61141 cccgatagtg tgctgaattc tctccataag atttcaggat ctgtttgcat caattggagc
    61201 tctctttcga tttcccatat ctcaaaatcc gtaatatcat ttaacacgta ataggttaaa
    61261 cgttctttat cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg gattacttgc
    61321 ccttgataag gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc tcgaccgttt
    61381 cgtccgataa gtcctagttt ccattgtgtg tcaaaattta gatttgcttg ttcaaataat
    61441 aatgtgcctt ggctgtcgta gccgaatgtc agatttttta tttcgatttt agacattttc
    61501 catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag gatgcaaggg
    61561 ttggatacac acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg aaaatctgca
    61621 cactgcttcc ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa tcgacttaat
    61681 ttttcaatgc acggatccct cactttgtcc tatatttgaa attacgatag cataaagaaa
    61741 atgattttgt caactacttc gggaggatgg tgaaaaatct gctaagtgtc tgtcctaaga
    61801 tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct attggaccca
    61861 aatgaatact taagacaatt gggagaaaat cagaaagtcc ttctagctct tggtctctac
    61921 ttccgcaaat agcgattatt ggttttcgat atttttttgc aagcactgct acaccgtatg
    61981 gaacttttcc gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg atgacatctg
    62041 cttgttcgat ctttttttcc aatccagtga tttggctgac taaatcgaaa ccagaaacca
    62101 tcttcgctcc caacaaaaaa agggctccac caataccgcc tgctgctcca cttcctggaa
    62161 tatcatctaa atccacctcc cagaaatctt ttatttgctg agcaatcttt cctgcttgtt
    62221 tatctaagaa agctagcgtc gtatctattc ctcctttttg tcttccaaat tgccaagcag
    62281 ctccttgttg acctgtatat ggattggtaa catctgcagc gataatcaat tttactttag
    62341 aaacaagaac gtttcttcga tcaaaatgaa gttttttagt agataataaa ggatttcctt
    62401 ctgtgtaccc ttctaaagaa gcacccaagg attggagtaa tcctagacca ccatcgctac
    62461 acccgcttcc tcctaaagtg acaataattt gttctacttc ttgaagcagt gcatctttaa
    62521 taagttctcc taaggcataa ctggaagcaa gtcggatagt ttgatccgtt ggtttatcaa
    62581 gtagatctaa ccccaatgct tgtgcagatt cgatgactgc ggtgtttttt ccttgccaag
    62641 ttgtcaataa atagctgatt tttcgctgac ggaataagag atccatccca tcaatggtac
    62701 gccatgttcc cctgagcgcg taatagattg cttctgcagt attttctccg ccatctgcaa
    62761 tcggcacatt gatttttctt cctttacgat cttcccagcc atttagaact gcctgattta
    62821 gttcaaatga agaagcactt cctttaaatg aatcaattgc cgccaaaata tccatctgta
    62881 tccctcactt tcttcttaag taagacactt tttttcattt ttttcaaaca aaacaatgct
    62941 attttcttcc atttttctat atactaaaag ggaactcatt gaataaagga gagaaaaaac
    63001 atgaaaatcg caatagctgg ggccggagca atgggcagcc gaatcggatt gatgctacat
    63061 caaagcggaa atgaggtcct cttgatcgat cgttggcctg ctcacattga agccattcgt
    63121 acaaatgggt tgattgctga ctttaacggt aaagaagtgg tcgcaaaact gccaatctat
    63181 tcaccagaag aaatcatcga aagcaacgaa cacgtggact tgatcgtagc attaacaaaa
    63241 gccaatcaat tagatgatat gttttgttcg atccaatcca ttattactga caatacttat
    63301 gttttgtgtt tactaaatgg attaggtcat gaagatgtac tggaaaaata tgtgccaaag
    63361 aaaaatatct tatttgggat cacgatgtgg acagctggtc tagctggtcc cggaaaagtc
    63421 acattgttag gtgatggaga aatcgaacta gagaacttgg aaccagaagg cgaagcattt
    63481 acaaaaaaag ttgtagaagt ctttcaagaa gcaaatttaa atccgatcta tagccataac
    63541 gttcgttatt ccatttggcg aaaagcttgt gtaaatggaa cattgaatgg tttatgcacg
    63601 attttggatt gtaatattgc tgaactaggc gcgcaaaaag cggcagagag catggttcga
    63661 acaatcgtat ctgaatttgc ttctatcgca gcaaaagaag gaatcatact agatcaagaa
    63721 gaagtgtacc aacatattgc ttctacttat gatcctgaca acatcgggtt gcattaccca
    63781 tctatgtacc aagacctgat caaaaaccat cgtttgactg aaatcgacta catcaatggt
    63841 gccatttggc gaaaaggaca aaaatatgat attgctacac catattgtgc ctttttgacc
    63901 caattagtcc atgcaaaaga aggaatcctt ggcgcagaat aaaaggctct ctgatgagaa
    63961 tcagctgaat cagcagctgg ttctcttttt ttaagcccaa taaaagatca aaaagaaaac
    64021 acaactctca tgatgccata agaattgtgc ttgtttgttt cagtacataa tctcttcaaa
    64081 atgtaggttt tttatttgtt ttcaacagag gagaggtcat attcgtattg ctcataaata
    64141 gtggaatcgt tagtgataaa tcgttttgcc caattttctg catcataaaa ttccaatagc
    64201 tctgatagga ttggcttaaa tggtgtatgc agaaattccg gtaattcatc taaatcacgt
    64261 tctcgaagca aaaaaatatt ttcaggacga taaaaatgat aatgagcaat cgcttgatta
    64321 tccgtcacta gtttcttttg aaaatacatg gattcaaatg tccgcaaagt cactccttgc
    64381 tgaccctccc gcaacagttc cagaatcccg cgcgattccc ctgttttttt caggtactct
    64441 tcataagtaa gatatccagg ttgttcattc cctcgatgtg gaagaatgaa atagttggtc
    64501 gtaacatctc tttttataaa ttcctgctta tagctttctg ctcgttcttt tctcccatta
    64561 ttcgttgcac caaaaaatag atccgtcttt ggttcgctag aagaaaaatt catatgtttc
    64621 gaataaaatg aactattatg tttcagccca agttcttttg cttccaaaaa atcaaaatga
    64681 taaatctcat cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct
    64741 ttgaagtgat tccaaaaaaa taacacaatt tttgcattgg taaatgggcg cacgcgtcga
    64801 gccatctcca tatcaaatcc ataatctaaa aaaacgatca gctgataatt tgccaactgt
    64861 tgtctagctg atttaatgaa ttccgactgt agtaaatctt ttccggacca aacatctaaa
    64921 gagcggatgg aaggcaaaaa ataaagtgat ttattttttg caactagcag tacgtcattt
    64981 ttgttcatct tttccgcctc actttctctg tcactttttc ctatgatagc ctgtttaaat
    65041 agtaaaaagc cagtaaaacg tttcctatat ttgtatgaaa atttaacatt tgtatgaaaa
    65101 tatgatgaaa taaatcattc ttctattaaa attcgcctaa aagttttctg aatagttgta
    65161 attgtctgaa aatttgttat cattagtcat tgttaaatat tgctatttat tcttaatata
    65221 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
    65281 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
    65341 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
    65401 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
    65461 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
    65521 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
    65581 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
    65641 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
    65701 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
    65761 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
    65821 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
    65881 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
    65941 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
    66001 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
    66061 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
    66121 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
    66181 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
    66241 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
    66301 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
    66361 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
    66421 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
    66481 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
    66541 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
    66601 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
    66661 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
    66721 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
    66781 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
    66841 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
    66901 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
    66961 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
    67021 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
    67081 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
    67141 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
    67201 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
    67261 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
    67321 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
    67381 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
    67441 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
    67501 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
    67561 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
    67621 gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat
    67681 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
    67741 tcacaatccc tttttatgtt tt
//
LOCUS       DAFBQW010000008        63477 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFBQW010000008 DAFBQW010000000
VERSION     DAFBQW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.008"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(154..552)
                     /locus_tag="LK889_000718"
     CDS             complement(154..552)
                     /locus_tag="LK889_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK5094974.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="LK889_000719"
     CDS             complement(549..1322)
                     /locus_tag="LK889_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK5094975.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="LK889_000720"
     CDS             complement(1319..2002)
                     /locus_tag="LK889_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK5094976.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="LK889_000721"
     CDS             complement(2169..3056)
                     /locus_tag="LK889_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5094977.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="LK889_000722"
     CDS             complement(3053..3922)
                     /locus_tag="LK889_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5094978.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="LK889_000723"
     CDS             complement(4059..5345)
                     /locus_tag="LK889_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5094979.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="LK889_000724"
     CDS             5618..6196
                     /locus_tag="LK889_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5094980.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="LK889_000725"
     CDS             6196..7125
                     /locus_tag="LK889_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5094981.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="LK889_000726"
     CDS             7122..8858
                     /locus_tag="LK889_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK5094982.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="LK889_000727"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="LK889_000727"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBK5094983.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="LK889_000728"
     CDS             complement(9754..10263)
                     /locus_tag="LK889_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBK5094984.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="LK889_000729"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="LK889_000729"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK5094985.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="LK889_000730"
     CDS             complement(11564..12655)
                     /locus_tag="LK889_000730"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBK5094986.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="LK889_000731"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="LK889_000731"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK5094987.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="LK889_000732"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="LK889_000732"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK5094988.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="LK889_000733"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="LK889_000733"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBK5094989.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="LK889_000734"
     CDS             complement(16618..17592)
                     /locus_tag="LK889_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBK5094990.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="LK889_000735"
     CDS             complement(17573..19456)
                     /locus_tag="LK889_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBK5094991.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="LK889_000736"
     CDS             complement(19474..21699)
                     /locus_tag="LK889_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK5094992.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="LK889_000737"
     CDS             21814..22848
                     /locus_tag="LK889_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5094993.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="LK889_000738"
     CDS             complement(22915..23928)
                     /locus_tag="LK889_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5094994.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="LK889_000739"
     CDS             complement(24006..24194)
                     /locus_tag="LK889_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK5094995.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24634..24762)
                     /locus_tag="LK889_000740"
     CDS             complement(24634..24762)
                     /locus_tag="LK889_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK5094996.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24765..26138)
                     /gene="rlmD"
                     /locus_tag="LK889_000741"
     CDS             complement(24765..26138)
                     /gene="rlmD"
                     /locus_tag="LK889_000741"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK5094997.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26249..27304)
                     /locus_tag="LK889_000742"
     CDS             complement(26249..27304)
                     /locus_tag="LK889_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK5094998.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27314..28744)
                     /gene="gatB"
                     /locus_tag="LK889_000743"
     CDS             complement(27314..28744)
                     /gene="gatB"
                     /locus_tag="LK889_000743"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBK5094999.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28741..30213)
                     /gene="gatA"
                     /locus_tag="LK889_000744"
     CDS             complement(28741..30213)
                     /gene="gatA"
                     /locus_tag="LK889_000744"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBK5095000.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30213..30518)
                     /gene="gatC"
                     /locus_tag="LK889_000745"
     CDS             complement(30213..30518)
                     /gene="gatC"
                     /locus_tag="LK889_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBK5095001.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30624..32660)
                     /gene="ligA"
                     /locus_tag="LK889_000746"
     CDS             complement(30624..32660)
                     /gene="ligA"
                     /locus_tag="LK889_000746"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK5095002.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32677..34911)
                     /gene="pcrA"
                     /locus_tag="LK889_000747"
     CDS             complement(32677..34911)
                     /gene="pcrA"
                     /locus_tag="LK889_000747"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBK5095003.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35066..36625
                     /locus_tag="LK889_000748"
     CDS             35066..36625
                     /locus_tag="LK889_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBK5095004.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36736..38667)
                     /locus_tag="LK889_000749"
     CDS             complement(36736..38667)
                     /locus_tag="LK889_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5095005.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFISIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38685..39599)
                     /gene="pfkB"
                     /locus_tag="LK889_000750"
     CDS             complement(38685..39599)
                     /gene="pfkB"
                     /locus_tag="LK889_000750"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK5095006.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39596..40348)
                     /locus_tag="LK889_000751"
     CDS             complement(39596..40348)
                     /locus_tag="LK889_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5095007.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40507..40716)
                     /locus_tag="LK889_000752"
     CDS             complement(40507..40716)
                     /locus_tag="LK889_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095008.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40727..42055)
                     /locus_tag="LK889_000753"
     CDS             complement(40727..42055)
                     /locus_tag="LK889_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095009.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42191..42346
                     /locus_tag="LK889_000754"
     CDS             42191..42346
                     /locus_tag="LK889_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095010.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42420..43007)
                     /locus_tag="LK889_000755"
     CDS             complement(42420..43007)
                     /locus_tag="LK889_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK5095011.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43090..44340)
                     /gene="clpX"
                     /locus_tag="LK889_000756"
     CDS             complement(43090..44340)
                     /gene="clpX"
                     /locus_tag="LK889_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBK5095012.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44587..45876)
                     /locus_tag="LK889_000757"
     CDS             complement(44587..45876)
                     /locus_tag="LK889_000757"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK5095013.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46030..46962)
                     /locus_tag="LK889_000758"
     CDS             complement(46030..46962)
                     /locus_tag="LK889_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095014.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47119..47778)
                     /locus_tag="LK889_000759"
     CDS             complement(47119..47778)
                     /locus_tag="LK889_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK5095015.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47781..48806)
                     /gene="sppA"
                     /locus_tag="LK889_000760"
     CDS             complement(47781..48806)
                     /gene="sppA"
                     /locus_tag="LK889_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK5095016.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48819..49109)
                     /locus_tag="LK889_000761"
     CDS             complement(48819..49109)
                     /locus_tag="LK889_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095017.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49518..51323
                     /gene="glmS"
                     /locus_tag="LK889_000762"
     CDS             49518..51323
                     /gene="glmS"
                     /locus_tag="LK889_000762"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK5095018.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51395..52750)
                     /locus_tag="LK889_000763"
     CDS             complement(51395..52750)
                     /locus_tag="LK889_000763"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK5095019.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52774..53943)
                     /locus_tag="LK889_000764"
     CDS             complement(52774..53943)
                     /locus_tag="LK889_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095020.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53940..54827)
                     /locus_tag="LK889_000765"
     CDS             complement(53940..54827)
                     /locus_tag="LK889_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBK5095021.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55016..58708)
                     /gene="nifJ"
                     /locus_tag="LK889_000766"
     CDS             complement(55016..58708)
                     /gene="nifJ"
                     /locus_tag="LK889_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK5095022.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58907..59428
                     /locus_tag="LK889_000767"
     CDS             58907..59428
                     /locus_tag="LK889_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK5095023.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60127..60684)
                     /locus_tag="LK889_000768"
     CDS             complement(60127..60684)
                     /locus_tag="LK889_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095024.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(60863..61474)
                     /locus_tag="LK889_000769"
     CDS             complement(60863..61474)
                     /locus_tag="LK889_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095025.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSARYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(61587..61844)
                     /locus_tag="LK889_000770"
     CDS             complement(61587..61844)
                     /locus_tag="LK889_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095026.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(62441..62719)
                     /locus_tag="LK889_000771"
     CDS             complement(62441..62719)
                     /locus_tag="LK889_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095027.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(62753..63004)
                     /locus_tag="LK889_000772"
     CDS             complement(62753..63004)
                     /locus_tag="LK889_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095028.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        17687 a        13739 c        11222 g        20829 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatgcaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttctt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagatgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa acaataacaa
    24601 aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt tagggccttt
    24661 tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc ctccaccttg
    24721 tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc tcctttaacg
    24781 aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg ctgaactttc
    24841 ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt tgccggatta
    24901 catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat aaaatgatga
    24961 tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc tttttcttgc
    25021 cattttttca taactttttc tgcttttccg acttcataat gaacattttc gattttatta
    25081 tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat cccatacacc
    25141 ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata agcgtcgatc
    25201 acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag atactctgct
    25261 tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt tcctaatagc
    25321 tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa aatcacattt
    25381 gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc ttcatgaata
    25441 gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa aacgaccatc
    25501 atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc ttcatttttt
    25561 tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac tttgattgct
    25621 tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc atggctattc
    25681 tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat ctgtgcttta
    25741 ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac ttctggcatc
    25801 ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag ctgttgttca
    25861 tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt ttctacaggc
    25921 tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa cttattgcct
    25981 gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat aaataatgga
    26041 taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat ttcgactgta
    26101 tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt taagcctatt
    26161 tttcaatcct actgaactct actataaatc agctaattac acaactaaaa ccggcactga
    26221 ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt tcgtctgcta
    26281 tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt acgaattctt
    26341 tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca ggaatctgat
    26401 cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc ggtgcttccc
    26461 cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct tctgtttcaa
    26521 cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt cgaccgccat
    26581 tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc acaatgatta
    26641 aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat cctccaacag
    26701 agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct tcatcatact
    26761 tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc gcaagataag
    26821 caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat tctgtcaaat
    26881 agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg tccattttta
    26941 cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta ggaattttta
    27001 atgctcgagc atagtcgttc gttgtaccag ctgggatgat ggccatttta ggacggtgtt
    27061 caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca gctgccacta
    27121 ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa tctggttctg
    27181 gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt aggatatccg
    27241 ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg cgtgcttttt
    27301 tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc acgacaccag
    27361 gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca accgcacgat
    27421 cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt tcattaatga
    27481 ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca actacttctt
    27541 gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt tttgagctga
    27601 tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc aattttgtct
    27661 cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc aaccagttag
    27721 atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca gacatttctt
    27781 ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg atatagcgtt
    27841 cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc cattcgtcat
    27901 cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta gacccttctt
    27961 tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt tcttgttgga
    28021 tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct aaaccttttt
    28081 tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac tcttcttgtc
    28141 cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc attttcacat
    28201 cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg tatgcttctt
    28261 ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct tgtcggttca
    28321 aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct tcttctaaat
    28381 ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg atccatccgt
    28441 catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta tctgggtaaa
    28501 agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat gccaaagctg
    28561 ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct ggataacccc
    28621 agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc gctggagaga
    28681 aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact gtttcaaaat
    28741 tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg ttgcttgttc
    28801 aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga tgatctgcaa
    28861 tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa gtccagccaa
    28921 gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt taatgttctc
    28981 accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat aatctgcaaa
    29041 gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt tgaagtaagc
    29101 gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt ttacttcttc
    29161 accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca cattttcaga
    29221 gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt ctgatgaggc
    29281 aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt cttccacaac
    29341 agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta cgccaggggc
    29401 cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga tgtcgcctgt
    29461 cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt tttcgtcata
    29521 accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca ttggtccgat
    29581 ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc cataagtagg
    29641 tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc cagtatcact
    29701 acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac cagaagagcc
    29761 accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag aagtttctgt
    29821 acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca tatctgcttg
    29881 atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa ggatcttaga
    29941 tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa ttggaatacc
    30001 agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct gttccaatgc
    30061 tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt tgattcgttc
    30121 aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca tatgatgcag
    30181 ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc attatcgatg
    30241 attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa taattcatcg
    30301 cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc aaccacattc
    30361 gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat atcgatgatt
    30421 ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag ttttgctaat
    30481 ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct ttctaaacga
    30541 gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt gtttattgaa
    30601 atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc caatgcatct
    30661 gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt cagcttgctg
    30721 ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc agtcactttc
    30781 ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt tagtttacca
    30841 gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg ggcatttcga
    30901 agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa ttcatggact
    30961 tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat cgtatcaagc
    31021 gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa atgttctgct
    31081 aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat caaccgctcg
    31141 accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga tttttctttt
    31201 attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc aacatctgct
    31261 actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc gatattcatc
    31321 gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca tttaggattg
    31381 atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca aactggacaa
    31441 tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac gacttgcgaa
    31501 acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa gcggatatcc
    31561 ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt tcctgctact
    31621 cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac tgtccattcg
    31681 atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc tgcccatctc
    31741 ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac tttgatgacg
    31801 attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc aatgtatgac
    31861 cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa tccaattttt
    31921 tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa atcagctact
    31981 gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa ctgacgcaaa
    32041 gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc ttctctgtct
    32101 tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac ttcgatgttc
    32161 attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc agtgatgttt
    32221 tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc ttcataacgc
    32281 aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac acgtcccact
    32341 gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac gtcgttcaaa
    32401 ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt tccgcctact
    32461 cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat tgctactaat
    32521 tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc tacgtagtat
    32581 tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc agcttcgtca
    32641 atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa ttggcgcaaa
    32701 ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt ctagatcctt
    32761 cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt tgactttatc
    32821 tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa caaccgctgt
    32881 ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt ttttaggcgt
    32941 aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa aacgagaagg
    33001 tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta aatacaacgc
    33061 ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca gttcgctttc
    33121 ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca ggaatacaac
    33181 tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg aagcatcttc
    33241 ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg ttagtttttc
    33301 ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga attcttgtgt
    33361 gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg tattttggat
    33421 ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt ctgtcactgt
    33481 caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac ctaattgctg
    33541 tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa gcagtggcca
    33601 ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga tgcctcgttt
    33661 gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca gcacattcaa
    33721 atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc caaccatttt
    33781 ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg cgttcgtccg
    33841 gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt cttgcatccg
    33901 gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag tgatcttgtt
    33961 tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt tttcaatgac
    34021 ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta gcaaaattac
    34081 tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg caccgcgcca
    34141 gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc tacctgccaa
    34201 taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta catgaatata
    34261 atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata atcgaatcgt
    34321 attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt tttggtaagc
    34381 agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg caaaatcctg
    34441 aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc gaggatcata
    34501 ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca gctgttcaga
    34561 agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc ttcttaaaat
    34621 acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag atgcgagaat
    34681 cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa tcgctaggat
    34741 gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg tcagcactct
    34801 cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt gaagaacagc
    34861 ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca ttaacaacat
    34921 cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta aactagctag
    34981 ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga tttcgcgcta
    35041 aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga aattagataa
    35101 gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag gaatcgtcgt
    35161 tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta tctatgaaat
    35221 gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc ttgcagcatt
    35281 tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga ttccacaagt
    35341 agtgggacag cttcgcgggg aattatcaat gaactggttc tctgttttat ggaaaaagtt
    35401 cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag gaccttctat
    35461 acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc catcttcaga
    35521 agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat tcaatgctcc
    35581 tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc cacttgtttg
    35641 gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact ttttcggctt
    35701 gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt atggttcctt
    35761 agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg tcttgctggc
    35821 tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt tcgttccttt
    35881 cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag gcaatcaaat
    35941 cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt tgtttgttct
    36001 tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga ttttccttcc
    36061 gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg ttgatttcgg
    36121 gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt atttcacagc
    36181 gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg gctctcttaa
    36241 tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg accttctcgg
    36301 cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc agaccgcctc
    36361 actaaaaggt gcaaggacaa cttttgattt tccagtaacg gcagagagtt ctttagacgg
    36421 attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta tacgcagagg
    36481 aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata ttttggttat
    36541 cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta aatcttctat
    36601 cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa caactattca
    36661 tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga aaacccagcc
    36721 tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata aaccataaat
    36781 cacaccagat acgactgctc cgatagaaat aaacaccaaa tacatgaccg ggtgactcag
    36841 taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga aagctccgac
    36901 taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa tcgggtcagc
    36961 tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga tgttcgtcaa
    37021 accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag caacgaaaat
    37081 tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac ttccgccagt
    37141 tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt tgattggacc
    37201 gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag cattcgaacc
    37261 actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag ggacattgac
    37321 cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga ggatcgtttt
    37381 aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata cgatgacata
    37441 accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg tattagctaa
    37501 tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg aagcaataaa
    37561 tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt tgaaccagct
    37621 agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc caataaattg
    37681 gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag gtaacatgtg
    37741 tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat ccgcatttcc
    37801 ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac cactaagggc
    37861 tagattgatc aactcttctg gtttacggat accatcactg acaggacgat tgatcagttc
    37921 ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta caccttctgc
    37981 acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt tcgtttcgac
    38041 tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg ccatgtaagt
    38101 gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt ctggatgagc
    38161 agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt tttgctcttc
    38221 agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta attttcctac
    38281 gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt
    38341 tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc
    38401 ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt
    38461 tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg cttcgcgggc
    38521 tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa acattttttg
    38581 gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga tcatcgcttc
    38641 tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc aatttttgtg
    38701 atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa
    38761 gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc tttcgtacgg
    38821 ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga atttttcaat
    38881 gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa agcaccgtct
    38941 ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt cccatacgga
    39001 atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc gtgattgttt
    39061 ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc tgttgtatca
    39121 atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata aaatcctttt
    39181 cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc tactgttcct
    39241 aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa gccattgatc
    39301 tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt tacggttgtg
    39361 aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc agtgaaccct
    39421 cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg ggaaacattg
    39481 atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc gcctagtttt
    39541 aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt gtaaatcatt
    39601 gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct aatggacaac
    39661 gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac aactggttga
    39721 atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct aaacgtttaa
    39781 cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca ccgttcattc
    39841 ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa
    39901 cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc tccaaatcaa
    39961 ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt ccttgcagaa
    40021 aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct tcgtcattaa
    40081 tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg agtgattttt
    40141 cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca tgaactcttt
    40201 ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat tcagaagcat
    40261 tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc aagtctagga
    40321 ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa ataatagcat
    40381 attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt ataaatcagt
    40441 caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt ctatcatcaa
    40501 gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag aagcaggttt
    40561 tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga ccaaaaaacc
    40621 gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata ataatgatgc
    40681 cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat attttttgta
    40741 aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga tgctgctcac
    40801 ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg aaggtaacat
    40861 cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct gaaggaacat
    40921 agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct acttctagat
    40981 attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca gtcggatcga
    41041 taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa tacgcctgag
    41101 catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata tcataacttt
    41161 ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca ttctgatact
    41221 ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac
    41281 cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa taggattgaa
    41341 acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg atacatatga
    41401 cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa atcggtttag
    41461 ggatgatttg atgataatag cgactataat acatttccca acacgaagtg aaacataaac
    41521 cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact tccccgtttt
    41581 gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat agccgttgag
    41641 agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag gcgaacaccc
    41701 catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt tctttgaaaa
    41761 cagagataga caatacattt tttatcaata attgattatc ttccaagcga aaactacatt
    41821 cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct agcattattt
    41881 tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag cggataaatg
    41941 ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt tttttttctt
    42001 cttcatttaa gtctgtgagc tggatagtca tcaataattt atcttcgcca atcattttct
    42061 cacctctttc aaatcttttt ataatttaat tataaccaat gataaagaaa aactaaaact
    42121 ttatgtctta gaaaccaatt tggacgatta catgatagac taagtaaaaa gaaacaaaga
    42181 ggtgtttaca atggggttga aatttgaacg tacagataat ttagataaac ttttcgaatc
    42241 atttgctgtc gatccggaaa aaacagttgc accaaaagaa gagatcgatc gatttctaaa
    42301 accacagcca caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta agctcaaaaa
    42361 aagacttacc agccaaactt ttatgaaaga gtttgactgg taagtctttt ttattctttt
    42421 tatgattcaa ttgcttgttc gattgctgcc cacgcttctt cttttccctg ttttgtaacg
    42481 gaagaaaaga gaataaacgt gtcattgcga tcaaaatcca acgctttttt gatttcgctc
    42541 tcatgcttgt tccatttacc acgagggatc ttatctgcct tagtggcaac aacaattaca
    42601 gggatatcat aatacttcaa aaattgatac atctggatat cttcttgaga aggcttatga
    42661 cgcaaatcta ctaaagaaac gaccgcacgt agctggtctc tagtcgttaa atatgtctcg
    42721 atcatctttc cccattttgc tcgctcagtt ttggatactt ttgcataccc atatcccgga
    42781 acatcgacaa aatgtaatgc gttctcgatc aaataaaaat tcaatgtctg ggtctttcct
    42841 ggcttaccgg aagtacgggc taaattcttt cgatcaatca atgtgttgat aaaagaagat
    42901 ttccctacat ttgatcgtcc tgccagagct atttctggca attgtgtatc tggatattgt
    42961 tttggtgcca ctgcacttat gataatttca gcttgatgga ctttcatggt ctctctcctt
    43021 aacaaggtga tgaataaagc aagcggctgt cgcaaaagcc aaaatgactt ttacggcagc
    43081 ctcttttcat caacccgctt ttttctctga atctttatag attactgttg gctcaccaga
    43141 caattcagct gcttcttttg tgataatcac tttttcgatg ttttcatttg aagggatatc
    43201 aaacatcaca tccatcatga tttcttcaat gatggaacga agtcctcgag caccggtatt
    43261 gcgttcgatt gcttttttcg caatggctct caacgcttca ggttcgaatt caagttcggt
    43321 attatctaga gaaagaagtt tttgatattg tttgactagc gcatttttcg gctcagtcaa
    43381 gatccgtacc agatcatctg ttgtcaattt ctctaaagct gccataacag gtaatcgtcc
    43441 gataaactca gggatcaatc cgaattttag taaatcttca ggaatgatat gctgcatgac
    43501 actttcgtct tcagaaagtt tttggttttg tgtaccaaat ccaatcgtct tttcgcccat
    43561 acgattcttc acaatcgttt cgatgccatc aaatgcacca cctacgatga acaatatgtt
    43621 cgtcgtatca atctgaataa attcttgatg tggatgttta cgtccgccct gaggtggcac
    43681 acttgcaaca gttccttcta gaattttcag caaggcttgt tggacacctt cgccggaaac
    43741 gtcacgagta atagatacat tttcgctttt acgcgcaatt ttatcgatct catcaatata
    43801 gatgatccct ttttctgcaa gttccacatt gtaatcagca gattgaagca atttaagcaa
    43861 aatgttttct acatcttccc ccacataacc agcttctgtc aaacttgtcg catctgcgat
    43921 tgcaaaaggt acattcaatg tttttgctaa tgtttgtgct aaaaatgttt taccagaccc
    43981 tgtaggtccg atcaagcaga tattgctttt ttgcagttca acatcatctg tgttttcttg
    44041 tgcttgctga ttgacacgtt tatagtgatt gtatacggca acagctaacg agcgttttgc
    44101 gcgatcttgt ccaatcacgt agttattcaa tacttcaaga atttctagtg gtttagggac
    44161 ttcagtgaat tcgcgtacag cttcttcata aaattcttca tcaatgattt ctttacatag
    44221 gtcgatacat tcattacaga tgtagacccc tggaccagct acgatctttt tcacttcttc
    44281 ttgggttttc ccgcaaaaag aacagcgtac ggtctcattg ctacttggat tgtcatacat
    44341 ggtcttcacc ccttaacttg caaaaagtat gcaagaaact atcaaagact ttacagtctt
    44401 ttactatcat aacatagttt agtaaaaaga aaaagtgttt aacaaaggat caactgacct
    44461 atacctttgc attttttact cttaacaaaa aataaggaac tgtgacaaaa tcgtgtcaca
    44521 gtcccttatt tcgaataaac aattttctag aagtagcttc ttcagaaatg atttattact
    44581 tgatttctat tctttcgctt ctgtcgctgt acctgtgatc aattcgattg cttttttcat
    44641 tgtcacatca tgttccagca tatcttctgt caacacgcgt ttgatttgtt cgattggcat
    44701 gttgtatgtt tctgataatt cttggatttc tgcattgatt tcttcttctg ttgcttgaag
    44761 attttcagca gctgcaattg cttcgatcac aagatttgtt cttgtgcgca tttctgcttc
    44821 accttcgaat tgtttatgca agtcttcttc tgtagaacca gttaattgat agtacatttc
    44881 aggagagata ccttgacgtt gcatgttgtt caagaattcg tccattgaac gatgtacttc
    44941 atcatgaacc atcacgtgtg gcaattcaac gatttctgcg ttttcaacag ccatacggat
    45001 cgctgcttca tcttttgcat cgtccgctgc tttttctttt gtttctacta attctttgcg
    45061 atatttttct ttcaattcgt ctaatgttga tacttcgtca tcaacatctt ttgcgaattc
    45121 gtcatctaat tcaggcactt cttttgcttt tacttcatgg attgtcactt tgaagactgc
    45181 ttcttttcca gcaagatctt ctgcttgata atcttctggg aaagttacat ttacatctag
    45241 actttcacct gatttatgac caactaattg atcttcaaaa cctggaatga atgagcctga
    45301 acctaattca agtgagtaat tttcgccttt tccgccatca aacgcttcgc catctacaaa
    45361 gccttcgaag tcgattacta ctgtatcgcc attttcagct gcagcatctt ctttgataac
    45421 taattcagct tgtgcttctt gttcacgttt gatacgtgca tccacgtcag catctgttac
    45481 ttcacggtct tgtttttcaa caactagatc tttatattgt cctaatttta cttctggttt
    45541 cactgttact tcagcagtga ttacccagtc ttgacctttt tccatgcttt caacatcgat
    45601 ttttggttga gaaacaggat cgatgccagc ttctttgaca gcagcttcat aagcttctgg
    45661 taatacagca ttcaatgcat cttcatataa agcttcttca ccgtacatac ggttgaatac
    45721 ttgacgagaa actttccctt tacggaatcc aggtacgttc aagctttttt tcactttatt
    45781 aaacgcagtt gttaatcctt tttggatttc gtcttgcgca atcgtaaatt ttaaaacacc
    45841 atcattcgtg cctttttttt caaatgttgc agtcatttaa ttccctccga gtttagaaat
    45901 tcattttata catcaaaaac gtttcatgca tacctttaaa tagtacacta accctttcaa
    45961 attgtaaaca tattgaatgg atagaatcct tgtttttcat aacattttta tctttttcac
    46021 tgataaaatc taatggaaga agatcatttg ctgcaaaatt ttttctcttt tttttaaaaa
    46081 atcttgtttt tcatcttctg taccttcata catcgttttt cctgtacacg cctctacagt
    46141 cagttgaaac cagcgcttcg ggtcttcgat gtatgtatca tagacaggat aaacaattgc
    46201 agattgcaac gtgaattctt cttttaacac ggaactaagc agcgcatctt cattcccgta
    46261 acgatctgaa aatacacata attgctggta acttgcctgc tgctgcggct ttggcagatc
    46321 ttttggtatg actttgacta acttttcttc aatcgtcaaa tagaaaacag gatggcattc
    46381 gttcaattgc ctaagtgtct ccagtaattt tgcccttgct aaaatatgga cctctggcga
    46441 tacaataagt ttactcgctg tttggataaa ctcatgataa ggaagctgtt cgatctcatc
    46501 aatagacgca agctgacgtg caggttccaa cgaaggaagt tcctccgatt tattgtggag
    46561 tgccttgatc ttctgcctct ccatttgtcc ggataattct tctgcaaatt ggattttttc
    46621 aatgacccgc tgctcgaaag cttcataaaa atctgtactt gctatcaatt ttctagcata
    46681 taaaaaatct tgtgtactga ttaacaagtc aaaataaaat tcagcaaact cctcattttg
    46741 aagatactgt ttttcatgca agagtgcttg cttcaatgct tcttttttct cgtccaactc
    46801 gtaaagacaa aaaacaagca ggctattagc ctcaaagctt tctttcaact cgtaagctcg
    46861 ttgaaataat tcttttgcct gctgatattg ctggtttgct agtgcttgtt gtgccactcg
    46921 cattaaattc tcatattttt ctggaaaatc aatcggcttc acgcagtgtc ctcctcttgc
    46981 tcatattcag ctcatgttca atagtagcaa aacaaaaaaa gcatgacaat taaaaataac
    47041 tgaagcaccc tctaaaagct agattttaga tctgactttt agaaggtgct ttcaactgtt
    47101 actgcttttt attgctcatc agctgttgac ttcctggttg aacgctttga tcagattgat
    47161 cgtaacaaca cttgtattag tgaaaaaatc gcccagatgt tgctttttct gattaaagac
    47221 tgctggaaga taccctaaca tcagcaaagg aaattttaga atgaaccggc aagcaccttc
    47281 tctaactaaa acggttgacc agctaagttc ttcttcatca aagctgatga ctcgaatacc
    47341 aaagatcatt ttcccaatcg tttggccatg attgaatttc gtcaataaaa taaaatacgc
    47401 tagataaata agtaaggctg tgaatccgta gagactcaga taagaatctg aaaaattaag
    47461 ccaacctaga tttttggcaa taccaagtgt taaacgcgta atagctccga tacacaaaag
    47521 atcgaccaag aaggcaaaca ttcggataaa aaaacctgca tagaaatact taggaaaatc
    47581 atttgctttt ggcggttcat ttcttgtgtc cgtatacttt ttccatttac gttgtttctt
    47641 tttgatttct tcgggcgtca aaggctctct ttcaaaagag ctcagtacct tttgggaaaa
    47701 gttggagtca ggtcctacag caggctgctg ttgcttatct ttttcttgtt ccttattttc
    47761 atttgaaagc tctgacatgc ttactcacct ccgtaataat acattgcttt tggtgattcc
    47821 gctgtcccta gattctcaag aacggataag atctgactag tctcagaagg cttcaatccc
    47881 tggaattctg ctagtttact tcctaaccat gtactagcaa acccagatgg cgtggaagaa
    47941 tattctacta gttccgcatc ttcaagctgt tgttcctttc gtatagcttt taaagcatct
    48001 tcaggaaatc ccaattcatc gactaaacct acttcttttg cttggacacc atcataaatc
    48061 cttccatcag caattttttt gacttcttct tcagatttat tccgtccctc gctgacgatt
    48121 gctacaaaac gactatatgc gctgtcgata tatgcttgaa gcaccgcttc ttcttctttt
    48181 gtctctggtc gcgttcctga acccatatct ttcaacgctc cgcttttaac tgttgtatct
    48241 tccacaccca gtttttctag aagtcctgaa taattcaatc ctgacatgat cacaccaatt
    48301 gagccggtca ctgtttcttc tgttgcaaag attttatctg cttgagcaga aatatagtag
    48361 ccaccgctag ctgccatgtt tttcatgctg acatacattg ggatatcatg ttctttacga
    48421 atcgtatcca atttcttagc gatttccgca ctttcgtaga caccgccacc aggagaattt
    48481 acttctaaaa agataccttt gactgttgga tcttcttcaa tcgccttcag ttgttccata
    48541 aaatttttat gattgtaacc ttcagaagca aataaacctg aagaccctcc cgcagcaatc
    48601 gttccatcta ctgataattt tacgattttt tgcgtactgt ctccctcttc caacacattt
    48661 ggagctagtt cttcatcccc atataaccaa ctgttcaatc cctcaatttg atcgttttcc
    48721 tgaggcttag tcaaactagc tgaaactaac gaaaccacaa ataatcctgc agcaatcaga
    48781 actgctgccc aacgtctttt attcattctt tctcctttct aatacaagtt ttttatgacg
    48841 atcacagttt ctgactgctc agaagtgtcg aaccgctctg ctttttcttc ccgcgcatac
    48901 ccggaaaacg actcttttgt taattcatct tctaccgtaa actcacgagc ttgagcaact
    48961 tccaaggcat caggtgcgat ttcttggatg ctatcccagc cagtatctaa aaatattttc
    49021 gtatcatctg atacaccagc tgatgcttcg aatttggaca attgtttttt taacattccc
    49081 agtgtcatga tctcaatttt tgaatccatt tttactcctc ctctatccga ttactttcta
    49141 ttgtaccgaa ctcctgaaaa agatgataga taaaaaccca ttattttttg acataaaaag
    49201 gacatttgac aaagaatttg atatatacca atttattttt tattcttttt ttgttgacaa
    49261 gaacattcgc aaaaggttat gattttaaat gtttccagat aaaaaacaaa aaggaggata
    49321 gttatacctt ttaggatagc gccaggtctg tgtaaacaga tgacgaggag agggtttatc
    49381 gaaagattcg gcggatggcc ctagggactg cactctacag aaaatccaca aaaaatgtct
    49441 gcaaagataa aacaaaagga ttttcctgca gctggaagca ttcatacgaa ttcattatag
    49501 aaagtaggat gttttttatg tgcggaattg ttggaatgat tggattaaaa aatgtaacac
    49561 caggattgat tgacggtcta gaaaaattag aataccgtgg ttatgattca gctggtatct
    49621 ttgtctcaga tggcacaact gattatctgg tcaaagcaca aggcagaatt caaaatttga
    49681 aaaacaaaat aaatattgat actacaggaa atataggtat tggtcataca cgatgggcaa
    49741 ctcacgggca accttctgaa gaaaacgccc atccacatac ttcccaaagt ggacgcttcg
    49801 tgctcgttca caacggcgta atcgaaaact ttgaagaact taaaattgct tatttagcaa
    49861 atgaccactt tattggtgag accgatactg agattatcgc tcatctaatt gaaactttcg
    49921 cgaaggatac tacaacgcaa gaggcgtttt taaaagcttt gcgggtcatc aaaggttctt
    49981 acgctttcgc tttgattgat cggacagcac ctgatgttat ttacgtcgct aaaaataaaa
    50041 gtccactatt aatcggactc ggcgatggat tcaatgtgat tgccagcgat gcaatggcta
    50101 tgcttgctca tacgaaggaa ttcgttgaga ttgaagatga agagatggtt accgtcacat
    50161 cagaaaaagt tattattcaa aattttgcag gtgacatcgt agaacgttct tcattcgaag
    50221 cacaagtaga tgcttcagat atcgagaaag gtacctatcc tttttatatg ctgaaagaaa
    50281 tcgacgaaca gcctattatc atgcgtcgta tcgctcaaaa atacgtaaca gaagacaatc
    50341 gtgtctctct tgatcaaaag ctggtcgata cattgtctga tagcgatcga atctatatcg
    50401 ttgcttgcgg aactagttac catgcggggt tagcaggtaa acagactttg gaaaaattga
    50461 cacagattcc tgttgaggtt catttagcca gtgaatttgg ctataacaca cctcttcttt
    50521 caaaaaaacc attctttatt ttcttgagtc aaagtggcga aacagcagac agccgccaag
    50581 tgctggtaaa aatcaaccgt ctcggctacc cttcacttac gattacaaat gttgctggtt
    50641 ctactctttc gcgggaagca tcctttacac tgctattgca tgctggaccc gaaatcgcag
    50701 tagcatcaac gaaagcttat actgcacaaa tagctgtact aactttatta gccaaagcta
    50761 tcggtgataa aaaagaattt gaaacttctc ttgtattcga tatcactcat gaattgagtt
    50821 tagttgctaa tgcgatggaa tcggtcatcg cccaaaaaga ttatttagaa gagttggcag
    50881 aagtatatct cagcgataca agaaatgcct tttacattgg acgtggtgct gactacaacg
    50941 tttctcttga agctgctttg aaattgaaag aaatcagtta tatccaggca gaaggttttg
    51001 ccgctggcga attgaaacat ggaacgattg ctttgatcga agaaggaaca ccagtgatcg
    51061 gaatcatcag tgaagaagtg actggtgcgc atactcgagg aaacctgaaa gaagtcgaaa
    51121 gccgtggcgc taaaacacta gttattgtct cagagggatt agaaaaggaa agtgaccagc
    51181 tggtgctacc aactgtccac ccttatctaa ctactctaac cacagtcgta ccgactcaat
    51241 tacttgcata ttacgcaacg ctattacggg gattcgacgt tgacaaacca cgtaatttag
    51301 cgaaatcagt gacagtagaa taataaaaga acaaggacca gtgtcagaca tctgacactg
    51361 gtccttgttc ttttattttt tatgaaaata tctcttaatc cagtccgatt tcttctttga
    51421 ctactgccgc gattttatcg acatagtaat taactttctc atcagtaggc gcttctgcca
    51481 tcactcgaag taatggttct gtaccggaag gacgaactag gattcttcct tctgatccca
    51541 tttcagcttc tgcctcttca atcacttttt tgattgccgg aacatccatc gcaccgtttt
    51601 tatcacttac gcggatattc actaattttt gcggatagat tgttacctct gctgcaagct
    51661 ctgataattt cttacctgtc tgtttcatga tattcagtaa ctgaattcct gaaagcatcc
    51721 catctccagt tgtgttaaaa tctaagaaaa tcatgtgccc agactgctcg ccgccaaagt
    51781 tataatcgtt tttgcgcatt tcttctacaa cgtaacgatc accaacttgt gtaacgacat
    51841 ctttcaaacc gattccttcc accgctttgt ggaatcctaa gttactcatg acagtcgtaa
    51901 cgatcgtatc ttttttcaat cgattttttt ctgccaagta tttagcacag ataaacatga
    51961 ttttatcacc gtcgacaatg tttccaagtt catccaccgc aataatacga tcgccatcgc
    52021 catcgaaagc tagtccagca tctgctcctt tttcaacgac catctcagct aaacgttctg
    52081 gatgggtaga gcctacgccg tcattgatat ttaacccgtc tggatttgtg cccattgtat
    52141 aaaaatctgt ttcaagatcg gcaaacaaac gattaacact tgttgaagtt gctccgtttg
    52201 ctgcatcgac acaaatggtc aaaccggaaa ggtcaccgtt gatcgtttgt acaaggaatt
    52261 gagaatattt caataagcct tctggaaatt cttctacagt tcccaatcct tcagctgatg
    52321 gacgaggcaa ttcatccact tcagcatcta ataatgcttc aatttctagc tcttggtcat
    52381 ctactagttt aaaaccatct gcaccgaaaa atttgattcc attatcttgt gctggattat
    52441 gagaagcaga aatcatgacc cctgcgcttg ctttttgcaa acgtgtcaga taagcaacac
    52501 ctggtgtaga gatcacacct agctggaaca cttcgatacc caccgataaa agtcctgaaa
    52561 tcagcgcttc ttctaacatt tgacctgaga ttcttgtatc ccgccctact aacacgcgtg
    52621 ggcgttttcc ttcttcttca tgctggctta ggacataacc tccgcaccgt cccagcttaa
    52681 atgcaagttc tggcgtaagt tccttgttgg cttcaccgcg tacgccgtct gttccaaaat
    52741 atttacccat agttgtaaaa ttactccttt aacttaattt tctgaggaat ttgttctgct
    52801 catttgtgtt gattcgcttg tttctgatgt ttcttctgta cttgtggtcg agctgcttgt
    52861 cccagattcc gaactggaag aaatggttgt tgtcgatggt tccgttcctg aatcagcaga
    52921 tgaaatactt ctactcgtac ttgtattttc ttgtgtccga ctgctcgtcg aagcagagct
    52981 actgcttgac gtttgcaaca cgggtgtaat ctccacacta actgattcgg gcttgatact
    53041 taacccctct tctactggta tttcaatttt acgagttgtt cgatgattga tacctgagac
    53101 attgatcggt acagcaatac tgtcaccgat cgatgcaagc aaatcgctag agccgcttac
    53161 ttctgcttcc agttttgaaa aactgaaatc atagctttct accccggcag ctggtgttcc
    53221 ttgctgtatc ccataaagtc gtaccgactt gcttggcttg atcgaatcag ctctcactgt
    53281 tacttgcact ggatcagaga cgatacttaa tacttcccct gctgcattca gcgcctgtat
    53341 tgggacctca ctattaatcc catcatctgt gaccatcgaa gcgtccacat cagctactac
    53401 tcgtttgatt tctgccaatg ttttgtcacc tgtagtgata gagacttttt taggactaac
    53461 ggaagtatca tcaagttcat atccagatgg tgtgaacgtt gaaggaatca acgtttccac
    53521 atcgaaatct tttattacct ttttctcaac cgttactgta atagtcgatg gttcgatctt
    53581 tgctgtgaca gcactgctca aattttgaat ttttagcgga acttcatgtg ttccttcgcc
    53641 cagcttagtg atatctgctg tgacgcggaa catccgtgta tcttcgtttg cctctgcatt
    53701 caactgaact cggtttgcac tggaaagttt gacagtcact gcattttcat atccatggat
    53761 atagtagcgc tttgaatcat aagaaatctt gatcggcacc cccgtaactg tttgattata
    53821 cgcttcattt ccagaaagcg tatttggaac ggactggcca tttgcattga agtacagcgc
    53881 aatactgaac agcaatgcaa taaaactgta aagaatgctt gtccgtttct cttttgagat
    53941 catttgcgat cccctccctt agtcataccg tctaggaaag attgaatcac gtttttcttg
    54001 ttttttgttt tctcttcagc aattagttct ctacgcaaga ttgccaaata ttcttgctgg
    54061 gataagccag ccatcagttc attatctaac gtgatgctga cgtccccggt ttcttcagag
    54121 acaacgatgg taatcgcgtc actgacttcg cttataccga cagctgcccg gtgtcgagtc
    54181 ccgaattctt tagggatcaa catactttct gatagaggaa gataggcgct tgctactgcg
    54241 atctttcctt cttttacaat aactgcaccg tcatgcagcg gtgtattagg aataaagata
    54301 ttgatcagca gctccccagt gatatctgca tctaaagcaa tccctgtctc aatatactca
    54361 tctaatccag tatgacgttc gatagtgatc aacgcaccga tttttcgctt ggacatatat
    54421 tggatggctt tgtcaaaaga caagatcatc ttctcatctt cctgctgttc gctttttgat
    54481 tgtttgaaaa gcgaactgcg tccaagatgt tccagtcctc tccggatttc cggctgaaaa
    54541 atcacgatag cagctatcac cccgtacgta atgacttggt tcatcagcca agacaaggtg
    54601 tgcagaccaa tgatttccgc tacgattcgg ataacaataa aaatagctac acctttcaga
    54661 agctgaatcg cttttgtgcc tcgcagcagt tggatcagtt tgtatacaag ataccagaca
    54721 accagaatat caagtatgtt caccaaaaaa ttcagagaga acaagtcacc ggaaaacatt
    54781 tgctgccaat agtccaaatt aaaaagttcc ccgatttgaa atgacataaa aagcctctct
    54841 tctcttttga gatttcctct ctagtatatc atatgtttta attttagagg ctgatatgct
    54901 cgtatttttc ttgagaaaaa cgcataattt tcaagaaaat tgcaagtatg cgttttattt
    54961 ttctctgcaa taaatatttt taaacttcag agaattattc attatatttt ttctgttatt
    55021 tttccttttt ttcttttgta gcttgttcca tttttttacg tcgttctgct ctggcagctc
    55081 ttctagcagc tcttctttct tctgcttcaa ggtccgtcgt tttttctttt ctagtcttaa
    55141 cgggtacatc gatagattcc tctggttcaa gtcgtttctt gattttttct aattctcccg
    55201 tcagtgcagc atagttgtaa aaacgtgttt tagcgtcgtg gacagtcttt tcaaataaag
    55261 cttttgcaag atttggattt gcgttatcta gcgaggaaaa gcgaacttgt ttcctcatga
    55321 aatcaggcat agcagagaaa tcaggttttt tgtagtccaa agtcattggg ttcttgtttt
    55381 tttcgaccat tgtagggtta tatcgatata atgaccaata accagaagaa acagcttctt
    55441 ttgcttcttt aagcgattct ttcattccgc caagcaagcc atgtgtgata caaggcgtat
    55501 aggcaataat cagagaagga ccaggatact tttctgcttc ttcaaatgcc ttgatcgttt
    55561 gcatttgatt ggcacctgaa gcaatttgcg ccacataaac atttccatat gtcatcgcca
    55621 tcattcctag atctttcttt gaagcatact ttccactagc cgaaaattta gcgattgccg
    55681 aagctggtgt tgcttttgag gtttgaccgc cagtattgga atagacttcg ttatccaaaa
    55741 caaggatatt cacgtctgct ccgctggcaa gtacatgatc gatcccgcca aaaccaatgt
    55801 cgtaagccca tccgtctcca ccaatcatcc attggctgtt ttttacgaat agatcctttc
    55861 tcttgtatat atcctccagt tcagggaaaa ttgatcgttc gctttcgatc gcagcttgca
    55921 gttttgctgc tctttgctgc gttccttcac tctcaaacat gtgtactaac cagtcttcca
    55981 tcaattctcg tagttcttct gatacttttg gcaatacttc tttgattgaa cttgccaaag
    56041 cctcgcgacg agtctgtgcc gctagaagca taccataacc aaattcagca ttatcttcca
    56101 ataaagaatt tgaccatgca ggcccttgac cacattcatt ggtcgtataa ggagaagcag
    56161 gagaagcacc gccccaaata gaagaacagc ctgtcgcatt cgcgatcatc attcgatcac
    56221 cgaacatttg tgtcaataat tttacataag gtgtttcgcc acagcctgag caagcgcccg
    56281 agaattcaag aagcggttga ttgaattgtg tagacaagac agtattcttt cctttgactg
    56341 gatttgcttt ttgtcttaat gtcattgcaa aagcccaatt cacagcttgt tctttctgtt
    56401 cttcataagg cttcatcact aaagcttttt cttttgcggg acaagcctct acacacagac
    56461 cacaacctgt acagtcttcc actgaaactt ggatacggta ttttacgccg tctgcgcctc
    56521 gcatttctct cacgataaat ccttctggcg cttcttccat ttcctcttca tctgctaaaa
    56581 atggacggat ggcagcatga ggacaaacaa aggcacattc attacacatc gtacagcgat
    56641 cactaatcca ctcaggtact tctaaagcga cgccccgctt ttccatagaa gccgttccta
    56701 aaggcatttc gccagttgtc attccatttg ctacaagtgt accaacagag agttgatttc
    56761 cttcctgtgc attcactggt tcaagtattt cttgtatata accagtgttc cttttattga
    56821 ttttttcagg catctccaac gttctccaat ggtctgggat cgcgacttga tgaagcagtt
    56881 cggtcgtccg atcgattgct tgtatgtttt tttctacgat agccattgat ttatgtccat
    56941 agcttttcag ggcttcttct tttagcagcg gaagcacctc atcaaaagga atgatgtcag
    57001 ctaatttaaa aaaagccgtt tccattgccg tattgatccg gcgtcccaaa ccgacttctt
    57061 gggccaatct catggcattg atcgtataga atctaatgtt attttcagcc aagtaacgtt
    57121 ttaattttaa gggcagatgc gtttctaatt gttcgtcgct ccaaagagta ttcaataaaa
    57181 aaatgccacc tggcttcaat cctttgacca agtcatagct atgcaggtaa gctggtgtgt
    57241 gacaggcaac aaggtccgca tgctcgacaa gatacgctga acgtatcggc gtatctccaa
    57301 aacgaagatg agagacagtc agaccgccag attttttcga atcataataa aaatagcctt
    57361 gcgcatattt atcggtgtga tctccaataa tcttgatagc tgatttattt gctccaactg
    57421 ttccgtcaga tccaaatccc caaaacttgg cctggaatgt ttgcggttct gtcaaatcca
    57481 gactttcctt tggttccaaa gattgataag tcacatcatc cacgatccct atggtaaacc
    57541 gttttttgcg tatgcttggg tcttttttca attcatcaaa aactgctgag atttggtcgg
    57601 gagtcacatc tttagacccc aatccgtagc gaccaccaat aacagccggg cgaagttcag
    57661 aatcatataa cgcactttga acgtctaata agagaggttc tccacctgcc cctggttctt
    57721 tacttcgatc aagaacagca attgttttta ctgtatgagg caacttttgc acgaatgttt
    57781 ccactggaaa aggacgataa agatggatat tcaaaaaacc tgttttccgt ccgtttgcat
    57841 tcaagtaatc aatcgtttgt tcaatcgtct gtgcaactga tcccatagaa acaatgactt
    57901 cttccgcatc gtctgctccg taataagtca ccaagtcata agaagttcct cgtaatcggt
    57961 tgatctcttt catatatttt tgaacgattt caggtaattt atcataatag gggttgatcg
    58021 tttcccgttg ttgaaaatgg atgtctggat tctggttcgt accactaaca gtcggatgat
    58081 tgggattcat ccctcgttta cgaaatgaag ctaatttctc ctgattgatc agaggagcta
    58141 attcttcata ttccaaaact tcgatttttt gaatctcgtg actcgtacgg aatccatcaa
    58201 agaaattcaa aaaagggact tctccttcaa tagaagcaag atgggcaaca ggtgctaaat
    58261 ccatcacttc ttgtacactg ctttcagcta acatagcaaa tcctgtttgt cttgcagcca
    58321 tcacgtctcc gtgatctccg aaaatattca acgcatttgt cgtcactgcc cgactagcaa
    58381 cgtggaaaac acttggcagt agttccccag caatcttgta catattcggt atcatcaaaa
    58441 gcagcccttg tgaagcggta tatgtagttg tcagtgcacc agttttcaat gatccatgga
    58501 ccacaccagc tgctcctgct tcggattgca tttctactac ttttactggt tgaccgaata
    58561 cgttttcttt tcctgctgct gaccattgat ctactaattc agccatggtc gaactgggtg
    58621 ttattggata tattgcagca atctcggtaa atgcataaga aatatatgcg gctgccgtat
    58681 tgccatccat tgttttcatt tttcgcatga ttcatcatcc ttgttcatat tctctcttat
    58741 tatctgttcc attataacgt aaaacatgcc atttttttct tttatttata aaaatatttt
    58801 acatgtatct atttgaattg gtcatctaat ttttagacaa acagtctaaa tcgttgcctt
    58861 tttttgtcag acatgtataa actataagcg aaagaggtgt tttaacatgg atatatcagt
    58921 gattgatgca acgaaaacaa atacacaaaa aggaatcctt tatggttcat cgaatgctcc
    58981 taaaaaaatg gtcgaattca tcaacttagc ttgtccgtat tgccgacaat ggtttgaaga
    59041 atcttatgag ctattagaag aagcagtcca aagcgggcaa ttgcagcgag tgatcaaatt
    59101 gttcgataaa gaaaaagaaa gcttacagcg agggaacgtg atgcaccggt atctcacaat
    59161 cagcgatggc caaaaagcga tcaaagagat caaacaaata ttcgatactc aagacgagtg
    59221 gaaacatctt tctctccaag aagtagcgga ttttgcagta gataaactaa agctgacaga
    59281 acaaaaagac gaacagctga gccaagctgt catcaacgaa gctgagcaag cacatattcg
    59341 atttgttcca actgtcatct tgggtaaaga aatctttgat gaatctattt caatagagga
    59401 attaaaagaa ttgatccaaa caaaataaaa aaataaggag cgtgggacaa aagtaaagaa
    59461 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttctta
    59521 tttcttggag ttaaacgctt ctgtcccagc ctccttattc cgaataaatg gtgtccaaaa
    59581 gcttgcatct gccagattat ctcatttctt atgacaagct aagcaaatta tctccaccaa
    59641 ataatttatt catatttttg acttgcgcat actaactaat tcttaaattt tcatacaaaa
    59701 tttctcaatt tcccttaacg agtaatattt atgtacggtg attctcacta gtatcccaat
    59761 cactttttaa gttatcccga tttcaattct acttcaaaaa tagttgcttt tatgtatgct
    59821 gttagttgat tagatggtca tttgttgagc taggtcgttt attttaaaca agttgtttct
    59881 ttagcttata cctatctcgt ctaccaaacg caagatggca aacacaaatg catacgcatc
    59941 tgtatttgcc actttgattc gtataaagct aacaactatt aactcctctc agctaatagt
    60001 tgctctcttt tttattcaga ttcatttttg ctgatcacag cctagtattt caatggtttt
    60061 gaacactaaa ccaaattgtt ccattacaac attcactggc tgatgaaatg gattagtaga
    60121 tttcgattat tcaaagcgat tttctattgg tggatcgtgt tcccaactaa taagacggat
    60181 tccaaaccta tcgtacagat cttctaaacc acttataaat tttgcaccta tttcaggtgt
    60241 tttattgcgt ggatattcta ttgttacgcc attaaatgga taccaattag gattaggctc
    60301 tctttttttc tgtacatctc ccatagaata gttagcactt tctgggacat aatatattct
    60361 tcgaccatcc tctaattcat ctaataagat atttcgtgat cgtctatcac ttgctaaatg
    60421 tagacgccac gtgcgatacc atgccatgct acctgcatct gcagaaaatc catcaaataa
    60481 tgcaacaatt gtatcttcta tcacaatcct ttccccatct acttcatatt ctaatctaaa
    60541 atcaaactcc cctcgggtaa tttctggtct tggtggctta ggacccaata ttattctact
    60601 taaaaccgca ttcgttccca gatgaaacac agctataaca acaataatga ttaatatttt
    60661 gtttctattt gataaatttc tcattattcg cacatcacct ctacattctc acatattagt
    60721 tcgtaatact cctttaatat caaaatatct actggtcgta ataatattcg ctatgaattc
    60781 ctactactcg tataagaaag gccaaatcat cagcttttac ttgctgatga tttggcttgt
    60841 ttctatttat cgtaattgtt ttctaatcac caaaattgtt aacaatcggt tcagatattt
    60901 cccagttgat taaggtaatt ccgtattgtg tcaaaacttc ttgcgcttct ccgaaattaa
    60961 gtacagttct tatgtatcca ctttctccta atataaagtc tacctctctt ttaaaacctt
    61021 cctcgagatt tctaagggct atatgtggtt ttaatgacat tgattgtaca ttttccccca
    61081 tataatatcg cgcacttcct cgactaaaat agattcctag atcgtcccca aaaggtagat
    61141 cttctctacc actagctaat ctacttctcc atcttcgttc tcttttcata gaacctgcgc
    61201 tgaactcaca cccgttaaac tcagctatga cagtatcttc aattatgtta atctgtccat
    61261 ctagttcgta ttcaatccta aatggaaatt ctccataggt gatttccggt tcgggtattt
    61321 taggtgagat taatgcaaag aaaggaaata ttaagcttgg caacatcaaa aaaactcctc
    61381 ctatgatcac aattcccgat gtaatccaaa atataatttg tacaattctc attgatccat
    61441 tgtattccat tttattcccc ctattactat tcatatccgc ctatctatat cgatcttcca
    61501 atgcagagat aattgcttcc ataccttgat tttttactga gttacctatg cgagatatca
    61561 ttctccacta aggtgcatca tattgtctat ctaacatgat taattctcca ataggtgtat
    61621 actcctccaa aaacaacatt taatgcatct ccctcgacaa cataagctac catgtttgct
    61681 gtatatgtgt ttgagtctaa ttcataagca gataaattta ctgtcgctgg attaaatata
    61741 atcgcattcg tatttatagc tactgaattt gctgcttcag caccaccttt tgaatgtccg
    61801 atcattgtga tttcaatagt aggatttacg tggacagccc tcaccgttta ccctgaccat
    61861 tatagaaatc gcttactttt ttgaactacg caacaaagga tacaatgctc ctaacttcac
    61921 tgtctccatt tttgtccact aaggaatata aaaaataggc attaattcat ttaattcggt
    61981 cacaacacgt taccttttaa atcattatac tgtatctcat acattattat caataaacta
    62041 ttataaaaat gcaaaaaatg ctttatcaat ctattttgta attggtcata atatcattga
    62101 aatcataccc tgtatcgatt tttcctatga atctcgtcta tttgtgattg acgaaaaaga
    62161 ggcaagacag acactggaat ggtaatttta cccactattt tgctccaact atttgtaatc
    62221 gtacccactt ttgaaatacc gagatgatca ttttaattga tatgctgatt cccaagcaaa
    62281 accccaagaa attaaatgct ctatcgcatc tagcttcttg ggttttgctt atataaattc
    62341 caacatttaa attttttatg ctgttcggtc tcagaaatgc cgtttttgct tatatcacaa
    62401 tatcaagatg tcaaattatt cgtgttgccg atgactttga ttaatttttt tcaaattgtt
    62461 tattaagact aatgctgtct ttttgcagat accaatgttc gaacttttta acattatcat
    62521 atgatcctgc aagttttaac ttcttaccat tagatgttat caaagttata attaaccttg
    62581 cctttttccc attaggatct tttattttat gaattttctg caggtaatct ctcctaatgg
    62641 cagctatttt tacctcatca gtgctgataa accattgatc atccacatac atactgcctg
    62701 ttatgttgtg tttttccatt tcttccgaga acgaaaagtt taaggctcgt tcttacttcc
    62761 aaagttgaat gagtccatgg attggactta gtgtgagaat tgagaacaac aataagaatc
    62821 catatcctcc cgcaaacatc cctaaggcta tactgtctct ccatccataa gtaggattag
    62881 aagagaaaag agtcaccgtt tgtaataata tgaaaaggaa taatccagca caaacaccaa
    62941 agataacaac tccaaacatt gaatttttat acaatgacca tttatttata ggcttttctt
    63001 tcattttact tccccctact gttctgttca cacggaatga attcaaagtt cataagccct
    63061 cctctttgaa catatgaata cgacttgcta actatacttt aaaaaagctt gttttaagcg
    63121 gttataggcg agttgtaatc tctattcatc tcattagcaa ataacttgcc gaaaagtcta
    63181 ctgaaattaa agcagatagt ggaagaatta tttagccagg atagacacta tttgtgaaac
    63241 acatgtcttt acttttgaaa ttctatgatg aaaagccttg gaatacagta gctcttaatt
    63301 ggtaatactt tttccattat aaataacctc ttttcatttt atatttatcc cctctaaaaa
    63361 taatatctaa tctaatattt taaacttata tttccctcgc tgtgctaata gagcattact
    63421 aaataaatag aaactagaaa aaagcattac gcctgccaga ataaaccaat tatttgt
//
LOCUS       DAFBQW010000009        63202 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFBQW010000009 DAFBQW010000000
VERSION     DAFBQW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.009"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(978..1151)
                     /locus_tag="LK889_000773"
     CDS             complement(978..1151)
                     /locus_tag="LK889_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095029.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1154..1552)
                     /locus_tag="LK889_000774"
     CDS             complement(1154..1552)
                     /locus_tag="LK889_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095030.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1768..2823
                     /locus_tag="LK889_000775"
     CDS             1768..2823
                     /locus_tag="LK889_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBK5095031.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2843..3196
                     /locus_tag="LK889_000776"
     CDS             2843..3196
                     /locus_tag="LK889_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095032.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3378..3674)
                     /locus_tag="LK889_000777"
     CDS             complement(3378..3674)
                     /locus_tag="LK889_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095033.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3678..4496)
                     /locus_tag="LK889_000778"
     CDS             complement(3678..4496)
                     /locus_tag="LK889_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5095034.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4722..4943
                     /locus_tag="LK889_000779"
     CDS             4722..4943
                     /locus_tag="LK889_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBK5095035.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4945..5211
                     /locus_tag="LK889_000780"
     CDS             4945..5211
                     /locus_tag="LK889_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5095036.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5324..5863
                     /locus_tag="LK889_000781"
     CDS             5324..5863
                     /locus_tag="LK889_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095037.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
     gene            6072..6254
                     /locus_tag="LK889_000782"
     CDS             6072..6254
                     /locus_tag="LK889_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK5095038.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            6357..6641
                     /locus_tag="LK889_000783"
     CDS             6357..6641
                     /locus_tag="LK889_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095039.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6657..6893
                     /locus_tag="LK889_000784"
     CDS             6657..6893
                     /locus_tag="LK889_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095040.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6915..7169
                     /locus_tag="LK889_000785"
     CDS             6915..7169
                     /locus_tag="LK889_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095041.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7251..7436
                     /locus_tag="LK889_000786"
     CDS             7251..7436
                     /locus_tag="LK889_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBK5095042.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7503..7898
                     /locus_tag="LK889_000787"
     CDS             7503..7898
                     /locus_tag="LK889_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK5095043.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7888..8265
                     /locus_tag="LK889_000788"
     CDS             7888..8265
                     /locus_tag="LK889_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095044.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8287..8661
                     /locus_tag="LK889_000789"
     CDS             8287..8661
                     /locus_tag="LK889_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095045.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8968..9366)
                     /locus_tag="LK889_000790"
     CDS             complement(8968..9366)
                     /locus_tag="LK889_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK5095046.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9558..10325)
                     /locus_tag="LK889_000791"
     CDS             complement(9558..10325)
                     /locus_tag="LK889_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK5095047.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(10360..11583)
                     /locus_tag="LK889_000792"
     CDS             complement(10360..11583)
                     /locus_tag="LK889_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBK5095048.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12542..13345
                     /locus_tag="LK889_000793"
     CDS             12542..13345
                     /locus_tag="LK889_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK5095049.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13930..14385
                     /locus_tag="LK889_000794"
     CDS             13930..14385
                     /locus_tag="LK889_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095050.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            14504..15562
                     /locus_tag="LK889_000795"
     CDS             14504..15562
                     /locus_tag="LK889_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBK5095051.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15604..17553
                     /locus_tag="LK889_000796"
     CDS             15604..17553
                     /locus_tag="LK889_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK5095052.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            17556..21197
                     /locus_tag="LK889_000797"
     CDS             17556..21197
                     /locus_tag="LK889_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095053.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            21597..21935
                     /locus_tag="LK889_000798"
     CDS             21597..21935
                     /locus_tag="LK889_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095054.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            22045..22266
                     /locus_tag="LK889_000799"
     CDS             22045..22266
                     /locus_tag="LK889_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095055.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            22286..24442
                     /locus_tag="LK889_000800"
     CDS             22286..24442
                     /locus_tag="LK889_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095056.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            24454..24687
                     /locus_tag="LK889_000801"
     CDS             24454..24687
                     /locus_tag="LK889_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5095057.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            24677..25543
                     /locus_tag="LK889_000802"
     CDS             24677..25543
                     /locus_tag="LK889_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095058.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            25558..27528
                     /locus_tag="LK889_000803"
     CDS             25558..27528
                     /locus_tag="LK889_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095059.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            27695..27922
                     /locus_tag="LK889_000804"
     CDS             27695..27922
                     /locus_tag="LK889_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095060.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            27919..28413
                     /locus_tag="LK889_000805"
     CDS             27919..28413
                     /locus_tag="LK889_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095061.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            28588..28923
                     /locus_tag="LK889_000806"
     CDS             28588..28923
                     /locus_tag="LK889_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095062.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(29180..30763)
                     /locus_tag="LK889_000807"
     CDS             complement(29180..30763)
                     /locus_tag="LK889_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095063.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            30885..31469
                     /locus_tag="LK889_000808"
     CDS             30885..31469
                     /locus_tag="LK889_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095064.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            31480..31725
                     /locus_tag="LK889_000809"
     CDS             31480..31725
                     /locus_tag="LK889_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095065.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            31731..32453
                     /locus_tag="LK889_000810"
     CDS             31731..32453
                     /locus_tag="LK889_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095066.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            32584..33327
                     /locus_tag="LK889_000811"
     CDS             32584..33327
                     /locus_tag="LK889_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095067.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            33333..33698
                     /locus_tag="LK889_000812"
     CDS             33333..33698
                     /locus_tag="LK889_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095068.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            33714..34244
                     /locus_tag="LK889_000813"
     CDS             33714..34244
                     /locus_tag="LK889_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095069.1"
                     /translation="MSQNISAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            34406..34774
                     /locus_tag="LK889_000814"
     CDS             34406..34774
                     /locus_tag="LK889_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095070.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            34965..35225
                     /locus_tag="LK889_000815"
     CDS             34965..35225
                     /locus_tag="LK889_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095071.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            35843..36643
                     /locus_tag="LK889_000816"
     CDS             35843..36643
                     /locus_tag="LK889_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095072.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            36680..36862
                     /locus_tag="LK889_000817"
     CDS             36680..36862
                     /locus_tag="LK889_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095073.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            37052..37339
                     /locus_tag="LK889_000818"
     CDS             37052..37339
                     /locus_tag="LK889_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095074.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            37430..37669
                     /locus_tag="LK889_000819"
     CDS             37430..37669
                     /locus_tag="LK889_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095075.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            37797..38126
                     /locus_tag="LK889_000820"
     CDS             37797..38126
                     /locus_tag="LK889_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095076.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            38179..39010
                     /locus_tag="LK889_000821"
                     /pseudo
     CDS             38179..39010
                     /locus_tag="LK889_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39025..39342
                     /locus_tag="LK889_000822"
     CDS             39025..39342
                     /locus_tag="LK889_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095077.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            39345..39577
                     /locus_tag="LK889_000823"
                     /pseudo
     CDS             39345..39577
                     /locus_tag="LK889_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39692..40168)
                     /locus_tag="LK889_000824"
     CDS             complement(39692..40168)
                     /locus_tag="LK889_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095078.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            40294..41625
                     /locus_tag="LK889_000825"
     CDS             40294..41625
                     /locus_tag="LK889_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBK5095079.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            41650..42177
                     /locus_tag="LK889_000826"
     CDS             41650..42177
                     /locus_tag="LK889_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095080.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            42164..42727
                     /locus_tag="LK889_000827"
     CDS             42164..42727
                     /locus_tag="LK889_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095081.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            43145..43882
                     /locus_tag="LK889_000828"
     CDS             43145..43882
                     /locus_tag="LK889_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095082.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(43899..44315)
                     /locus_tag="LK889_000829"
     CDS             complement(43899..44315)
                     /locus_tag="LK889_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095083.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            44708..45955
                     /locus_tag="LK889_000830"
     CDS             44708..45955
                     /locus_tag="LK889_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK5095084.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSNPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            46092..46235
                     /locus_tag="LK889_000831"
     CDS             46092..46235
                     /locus_tag="LK889_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095085.1"
                     /translation="MLSTKKVINRCGDFNLGMGAKVITTPTYRGSFSSPNWYKEGVTR
                     DEA"
     gene            46225..48615
                     /locus_tag="LK889_000832"
     CDS             46225..48615
                     /locus_tag="LK889_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBK5095086.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            48664..50499
                     /locus_tag="LK889_000833"
     CDS             48664..50499
                     /locus_tag="LK889_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5095087.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            50568..51431
                     /locus_tag="LK889_000834"
     CDS             50568..51431
                     /locus_tag="LK889_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5095088.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNVNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            51421..52530
                     /locus_tag="LK889_000835"
     CDS             51421..52530
                     /locus_tag="LK889_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5095089.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            52869..53669
                     /locus_tag="LK889_000836"
     CDS             52869..53669
                     /locus_tag="LK889_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095090.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            53757..54563
                     /locus_tag="LK889_000837"
     CDS             53757..54563
                     /locus_tag="LK889_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095091.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            54795..55664
                     /locus_tag="LK889_000838"
     CDS             54795..55664
                     /locus_tag="LK889_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK5095092.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            55684..56031
                     /locus_tag="LK889_000839"
     CDS             55684..56031
                     /locus_tag="LK889_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095093.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            56044..57306
                     /locus_tag="LK889_000840"
     CDS             56044..57306
                     /locus_tag="LK889_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095094.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            57386..58762
                     /locus_tag="LK889_000841"
     CDS             57386..58762
                     /locus_tag="LK889_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBK5095095.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            58759..59388
                     /locus_tag="LK889_000842"
     CDS             58759..59388
                     /locus_tag="LK889_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095096.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            59407..59886
                     /locus_tag="LK889_000843"
     CDS             59407..59886
                     /locus_tag="LK889_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095097.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            60388..60441
                     /locus_tag="LK889_000844"
                     /pseudo
     CDS             60388..60441
                     /locus_tag="LK889_000844"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            60445..61044
                     /locus_tag="LK889_000845"
     CDS             60445..61044
                     /locus_tag="LK889_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK5095098.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            61060..61536
                     /locus_tag="LK889_000846"
     CDS             61060..61536
                     /locus_tag="LK889_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095099.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            61553..62455
                     /locus_tag="LK889_000847"
     CDS             61553..62455
                     /locus_tag="LK889_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095100.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            62528..62722
                     /locus_tag="LK889_000848"
     CDS             62528..62722
                     /locus_tag="LK889_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095101.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            62827..>63202
                     /locus_tag="LK889_000849"
     CDS             62827..>63202
                     /locus_tag="LK889_000849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095102.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLE"
BASE COUNT        23327 a         9025 c        11887 g        18963 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac cttgcgtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgaacaagt ttttacctat tcccaaaatt aatttattag ttattttttc attgattaag
      181 aataattatt tgatgcttaa attagctaaa aaaacgattt tttactgata taatcatgat
      241 attaaagctg aattaggcgg aaagataaaa tgttcgcaaa atcaaagtat caattatctt
      301 gtttttttat aaatttgtaa gtaaaaatct aaaaagtttt cttttaattg gtatatgtat
      361 taaaatcaca ttcaatcact cataaaagta tgcgctttca tttttttgga tgagattaat
      421 atcaataaat aacgatgtaa aaaccattaa ttatgctctt ttataaggat attaattaat
      481 ctaatttgaa aaaataattc aaaatttaaa tgttttaata ttacaaataa aatcatgtta
      541 tcgtaaaatg gtataaaaaa gctaaatatt ctatttttgt tattttatta gcagggaaat
      601 ttaaatagtt tacaagaatc cgtagaaata tattggtttt gccttcagat taatactgga
      661 tcatattaat ctgaatataa tatatgaact aataaatagc ttaacctcgc tattttgttt
      721 tttctgatca ctatctatac agcattagta aatagaattt taaaaataaa aaaattaaga
      781 caaaaaaagt atcaacataa acatatacat atacacgttt atgttgatac acttaatttg
      841 tcctttaaaa agcacttatt ttaatattat gttattttat aatcttatta caagctgatt
      901 gttagacgtt gtcctttcat tacatttatt aacggttatt ttatcgttta atttattaat
      961 tatttttgat ctaaaactta gcttaacaaa taaataatta accaaccgcc agataaaaat
     1021 gcgatcattg ctaatacata agcaaagata aaaatatgtt cgatcagctt aaaccctgat
     1081 gcgattaagc agaccaagta aaatagaaat tctcttacag ataatggtaa ttttctaact
     1141 agcatactca taattaacac cccaaagtaa tattgtattt tgaacatttt acatccaata
     1201 cagcatcaat caaccattgc caatcaaaaa tacaaggctt cattgaagta tgtttttcag
     1261 atgaaaagat ataatataat ttcttcggaa taatattttc agttgttggg atcaagctaa
     1321 atttaatacc atctaactca tagctaccag ctttctctcc attaaaaatt tccttagcac
     1381 tttcttctaa agaatcaaaa tatttttcga cagcaacctt tgaagctttt actattgaag
     1441 catttccctt ttcgataaaa tctgcttttg ttttaccgac aataagtaat tcttgatcag
     1501 taaaatcttc taaaataata gtagtgtaat tcttatttct tctcaatcgc atcaggatca
     1561 ctccttaata taatttatgc ttttataata tcataataag ataaatttgc aagtgtttat
     1621 ttacaccttt ttatttacac tttattgtaa ttgattatac ttttttgatt gcattttttt
     1681 cttataaaca gttgcaacta ttagtaaata ggtgtaaact attttcgtac cgaaatacca
     1741 taaacacaaa cagaaagagg tcataccatg aaagaaccat tattgttttc cgttgactta
     1801 gggaataaat cagtaaaaga aatgagtaca cacgtttctc cactctcgct tccctctcgt
     1861 attttgaaca aaaatatcat taattcagga cgtttttcta atataggttc ctcattaaaa
     1921 cgatcactaa acgtttctga atatagccta gtaaacacta ctgaaagcta ttattttggg
     1981 aaggatatac acgagctagg aaaagatgac tacgtgatag aatcactagg ttttgggatc
     2041 agcagataca agcaggaaac ctaccaagat atcctatctt tctctttagc taacctagca
     2101 cgtttagaag atcaatctga tgacgtttta accgttaatc tagttttagg tctgccttca
     2161 tccgactata ccgaaacaat cgtcaaatac gtgatgcaac aagccacacg acaacattca
     2221 gttgaagttg acggtaaagc ttataatata aacgtagata ccgtttgggt actgccacag
     2281 ccgattggaa cactatataa cctcctcctt gctgatgatg gtagtgtaca agatgaatca
     2341 ttgatcagtc agaagatcgg ttttgttgat attggtggtg gcacgaaact ttttgatgtt
     2401 gtccaaaatt ttgaaattga cgaaaacaac cgtgaacaaa aggataccgg tatttttact
     2461 ttatataatc gtatcgtaaa tatgcttgac cttccgatca gtcctaatgc tttccaactt
     2521 gaaaagatta tccgagaagg caaagaaaaa ggcgtatatc tatttaaacc aagcggaaat
     2581 gaaagttttg atatttcaga agtagttgaa agagaaataa aaacatacac caatatggtt
     2641 gttgcagaag ttttagctac attcaaaaac ttcacaacct tagataaagt tgtctttact
     2701 ggtggtgggg cttctattat cgacaaacaa cttgtaaaag ataaaattcc gcataccttg
     2761 tttgttgaag acagtgaatt ttctaatgta aaaggctttt acaagtttgg tttaactcaa
     2821 taaaacagcg aaggagatca acatgaataa gggaaaatca gagcgttatg tagttataat
     2881 caatagagaa aaggttagcg agttaccttt agcagagttt cttgaatctg aatatgaaac
     2941 actaggcgtt aaatactctc agacgatcaa aaaagctttg aatctatatc ggcgtgtaaa
     3001 gaacaatgaa gttacaatta ttgaagcagg caacgataga aacgatagca aagtagctga
     3061 agtaaagaat aaaccaactg atcataaccc tattcaacaa gcgcatcctt caacggttac
     3121 atccgatcat tcagcaaaga aagaaaagcc aatgctaaaa gggttcaatg caccaagtaa
     3181 caaagactta ctttaaaaat aaaaaggcga ggttacaata cctagccttt ttattatgta
     3241 caaatacaaa cataaacata tacgtataca tgtatataaa catgtatacg tatatatcca
     3301 taaacacgta tacgaaattt agttaagcta tacctaaccg cttattccaa taaaaaaagt
     3361 gatcagcttt aaactgatca ctcaccaaaa acttcatcat acaagttatc tatcatttca
     3421 ccaagtaaag ctgaatctga tcgaattcct aacttttcgc tgattttaga tagtttcttt
     3481 ctttgtgatg gttgaagcgt gaattgataa tttttcttct tttcagaact atcacttttt
     3541 agtttagcta ccaaagaact tgttgtttct ttaggtgttt gtttaggttc ttcaactgct
     3601 tcaaccactt caggaacttc ttctacttca gcttcagcga attttttaaa tctattttct
     3661 ttcacttcaa tcactcctta ttcttgtgcc gtatcaatta ttttgttgta tagcgtaaac
     3721 gtgctttcaa agaaatcctt attcttacga tagatattct tatccttagc gaactcagca
     3781 atattcttac cttctaagct agttgcatta agcaattcct tctcttgtac atatccaaga
     3841 taattagata acgttggtac caattctagg aactcacgac ttgaattagt attatgtttg
     3901 atcctgctac cgatcgtagc gtatttagtt tccatcagtg tttcaccagt cttaatatca
     3961 atcaactgtt ttgctagtgc atcaatatct ttaccaagct taagcaaagc ttcaaaacca
     4021 tctttagatg gatcagaaat gcctacgaca agatcagata cagcccaagc attttgagct
     4081 agtagacctg catcattatg agtatcaatc agaattagat catacttctc tttaagctgt
     4141 tcatagttaa caacgatcca tctaaacagt agggttctgt tgttaggcat atcacgcatt
     4201 tgcatctcaa cttgttttaa atcttcacct gctactaaaa tatccatgtt ctcatttata
     4261 gctaaaggtt cagctgatcc atgaagatat aaatgattga ttgtattctc tcttttcgct
     4321 ttactgaaag catcaggaaa tcgttttgat aggttttgcg acttatcttc atcaatatac
     4381 agcaccttca tacctaaatt ttcgacagca tatccaccta gattgtagat tgttgttgtt
     4441 ttacctgttc cacctttttc aatgtttcca gtaatgattg acaacttctt ttccatgatt
     4501 aaacctccct tattaatctt ttgtaactcc attatacact ataaacacgt ttatgtatat
     4561 atttatatac ataaacgtgt ttatatttat atttgctttt atattctaca acataaacat
     4621 atatatgtat atgtttatgt ttatgtatat attcaaatat ataactattg catttgtcat
     4681 acaatcgtag tacaatgtaa ttgaaaagga gttgataaac tatgccagta ataagtattc
     4741 gtgtatctaa agaagaaaaa gattttcttg aaaaagttgc tgaattcaac ggagataaca
     4801 tttctgaatt tatgcgtagt aattccctgc aaacagcaga aacgttagta gactttgcca
     4861 cctacaagca actaatgaaa gagcatgagg aaaacgatca aagtatttcc cacgaagata
     4921 tgctaaaaga gttaggctta taagatgact taccaagtaa gatatgaacg tgaagctcaa
     4981 aaagcgctaa aaaaaatgga taagtttcag gcaaaggtta tcctaaactg gatcgagaaa
     5041 aatcttgtag gcactgatga tccaagaaag catggtaaag gtctaacagc taataaatca
     5101 ggctactggc gatatagggt aggtgcatac cgtataatag ctgatattag cgagcatgaa
     5161 gtgacaattc ttatattgtc tattggtcac agaagtgaag tttataagta acagacaagc
     5221 tgataaggct tgtctgtttt tttgttaacc caacaaaatc actgtttacg atcccacttt
     5281 acctaaaaaa gaacaatatc ctcttgatat tatacacacc agtgtgtata atataagtgt
     5341 aaacaagcta attaactttc aaaaggagcg attttttatg ggatggtatg gtgattattc
     5401 atcagcaaat gaagtgatcg aagaaaagaa agggtacgcc aaaaatggtt tggaaatcgt
     5461 aggtaccaaa gtaacgaaga cctatggaat tatccttttc aaagatgcag aaggaaccta
     5521 cgatattgac agcttcatct ttcgagatga aatgtacaaa ccaatctact tcttaaatga
     5581 cagtcctgct tactttaaga aaattccgaa aaaatggttg aaattctttg tcggtgatca
     5641 agaaaaaatg gttaagctac aactagatag aattaaagaa gaaagcaata aaccaaaact
     5701 tgatgactta ttagttgcag gtaaaactta ctcaatctgg gatggatcgt atgtcgctgt
     5761 ctatcagtat aaactcaaac gtagctttat ttttcgacta caagatggat cactcactcg
     5821 gttcactcga ctatcagcag gagatgttaa ggaagtaacc taaaacttaa atacataaac
     5881 ataaacgtgt atacgtttat gtttatgtat atgtatatga atataaacgt attaggtacg
     5941 aacactttta aattcttttc tgggctttgt ttttagggta gggctaaaaa aaacatcacc
     6001 atctgatcca aaacagctca gaaacgcatt taaacgtatc tgataccaat attacagaag
     6061 gagtgtatcg tatgattaac ctatttcttt tagtgacagc tagtacatta ttctttactg
     6121 gaatatcact gataatttcc tatttgatct ataagaaagc cgaaaatcac acaagcaaag
     6181 caatcggact agccgtttca tcctttttaa ctttgctgac tttatttgtt actttatcgt
     6241 tttcttttgt ttagaacagc tgatccatta gcaccttttt ctctttacat tatacacact
     6301 gttgtgtata atgtatttgt aaacaagcta attaactttt aggaaggagt gacaagatgc
     6361 taacagctac tttacagcaa cctactcacg ttgctattgc agaaagttat acaattaatg
     6421 cagggatcgt taaacagctg atcaaggatc gagaattcaa aaagtttaac agtcggcaag
     6481 cgtataacag acagctaaag aaagctattc taaagcacaa agattccttc atcgggaact
     6541 tggaaagtgt agatgttaaa actgtttcaa cggcattttc agttgttgaa gtgactattg
     6601 acggtcgctt gatttctcta tttgcttttg gaattaattg atagggagtg tgaactttgg
     6661 aaggtaagac ttttgcagaa atttggactg aatttattga ggtaccaact cgcattgatc
     6721 cgataacttc tgaaaaggta ttggatgcag acttctacaa tctcaaagca ggtactagcc
     6781 ttgaatgcgt ttggatgtat ttagaagagc atttcaaagg cgtaacgctc ggtcacgtct
     6841 tataccacaa tggtgccgag tgggatgcaa ttggaaatat ccatttttct taatataaga
     6901 aaggagaaca caatatgaat tataccgatg aagaacaaat cgctttggaa tacgttttca
     6961 caaatgaaga aggggaggaa attgaaaaag tcttgatcat ttcatcaaaa gacttggaga
     7021 aggctttggc ttatgaacca tacgaatcaa ccgtaactgt cattgaattc ttagatacct
     7081 atacttctaa tgatacttgg gaaattatga atatttgcaa cgctaataac attcaatttg
     7141 ctgaaaaaac caataaatca ttttattagt tttgcaataa aacatcctct ttttctattg
     7201 attccataca caacaatgtg tataatagat tttgtaaagg gagggtagct atgcctaaga
     7261 cagcaagaga aattgtaaaa attctcaaat ctaacggctt caaaaagact ggacaaactg
     7321 gcagtcatgc aaaattttat aattccaaga ctaaaagaac ggtcgttgtt ccgatccaca
     7381 atggtgatgt accactagga acagaagcta atatctggaa gcaagcaggg ctttaacagc
     7441 cttgccttcc tatttggtat taggcatact atcctctctt acactagaaa aggagcgagg
     7501 aaatgaagac aaaaaatatt ttttatccgg caatcttcga gaaagaagga gaagcttata
     7561 acatcgtttt ccctgactta ccggaaatat ctacctttgg aaatagtctt gaagaagcat
     7621 atttaaacgc taaggatgct ttaggattag cgctatatag tgtaccagat gcagaatttc
     7681 ctaaaccatc agcagtggaa gaattaactt tgaaagccaa ccaagtggta gtaatcatcc
     7741 aactaaacat taaacttttc cgaagaagct taaatacaaa aacaattaga aaaaatgtta
     7801 gtgttccgga atggttagtt cttttaggga aggagaaaaa cataaacttc tctcaactac
     7861 ttcaaaaagc actagaagaa gagttgcttg aaaagtaaaa aggagaccga acctatgaaa
     7921 gctacacact taaactattt ctctcgaacg ttaatcaata atctattaaa agatacaact
     7981 ggcattagcg ttagtaccaa acactttgcc aatgatctaa ttatttttga ggataataac
     8041 ttgcgaattg agatgtttat tgaacaggca acccctaact caatacctga attctgctat
     8101 acgcacactg aaaaaatgaa acatgcaaaa tcagttaaag atgttgaagc attaagtgaa
     8161 tcactaacga acgctttaaa cctcgttaag aaggcaaaat tgttatttga atttcaaacc
     8221 gactaccaca atgagttgct tgctcaatct gaaattttag attaattgaa acaggagaag
     8281 agaaagatga aacaaacaca aacagaaacc gttaacgata cgcttgctga tcaattagca
     8341 ggttattata aattatttga gatgggtaaa gcacagcgct tgatccacta tctttggaaa
     8401 tacaaaattt tgtttctttt ttgggaatca acactaacat tctcagttct tttttataag
     8461 tcagattttt ataatcaaca cttagtttta gggagttttt tgcttatcta caccattttg
     8521 atgcttaatt tatataccta catcacactt gaagaacact tagtaattag cgatgcaggg
     8581 atcgagagaa acatcaaaag ggtatccttc aagatcacgt taaacaagcc taccgataaa
     8641 aagctctcag aagcgttctg agagcttttg acattttctt gtataatttt atcctaacac
     8701 attacaaagc acctaattcg agcttatatc aagcaaattt gctatttatt actcaataca
     8761 tatctaaaaa ttacttgatg gaaaattaga tacatggtaa gcatgaaaag aaaatcaatt
     8821 tgagcagatg cagtacaata catagcaatt taaaagctct cagaaggctt ctgagagctt
     8881 ttaatatatt ctttgataat ttgtctttct taaatccata cgtcctaaaa caagctgttt
     8941 ttgcttaata tgatcatctg agagacatca aatcaacttt tctctcaaca aatcattcaa
     9001 agctttcgac agattaatat tctcttcagc cgctaaaaca tttaaatagt gttccacagt
     9061 cactgatttt cgatcataat tttgcttgtt tcgagcttta gcaactttcg tattagcttt
     9121 tacgacaaat aaattaacat tctcagatcg cttgattctt gcatttaact cttgaacagg
     9181 cgtaggcgat ggaatttctt tttcttgcaa ctctaaaagc accaagtatc cttccaatac
     9241 ttcttgcgcc atctcaaaag catcttctaa agtttcacca tgtgtatata agtctggaaa
     9301 atctgggaat ttaactgcat attcatttcc tttccattcg aatactgcac aaaaaaagta
     9361 tttcatcgca ttgcacctct ctaattaaaa aataattttt gcttttttgt ttcaaaaaaa
     9421 agaatcgcct accaaaaaaa ttgataggcg atcacaagct aattaacctc taaaggagat
     9481 taataccttg ctcccaaggc actatatatc ttagcatacg tctgaagcat cttcaaacga
     9541 gtaagatata ttttgcatta acctaatttt ttttctctat aagttttttc atcaaactca
     9601 ctagaaatat taaactggac agcaaaattg taatttctgc cttttctaac tttagcaaaa
     9661 tcaatattca gcattgattt ttcattaatt tcctcaactg attcccttaa aacacgtcta
     9721 tcaaaattcg atattgtttt atactcattt tctgttttcg taatctcacg aattttaact
     9781 atttcaatca ggggattctt atatatttca gctttctcaa aatctccttt atttttggcg
     9841 ataagatatt ttttataata catcgccaaa tatttgtata acacgatgct atattttttt
     9901 tgaaaagatg ctaagtcttc aaaaacaaag aacggatact tctttttgat tgagacaatt
     9961 aagggaatta catcttctga aaaactgaaa gatacactat gctcttctaa atcccaatga
    10021 atccgatcaa tagcagatac agtagtatac gtcttatatt ttttgtctag attcgaaatt
    10081 tcataaactg cttgtctttg caaagtgtta actatggttt ttagttccgt ttcaagattt
    10141 tgatcaacat ccatgtaatt aataatcgct ttaacaggta aatctactac gcctgttttc
    10201 tttagctttg tgggtgagat ataactgaac agcaattgat aaagttcata cgactttaaa
    10261 tccattttca tgtcaattgt tgctaagaac tcctcattgt actttgtcag ttcatctttt
    10321 ttcaaaagtt tttccttcct tcacgcattt tttcagttgt taatcataaa ggttcacata
    10381 aggcaaataa ctcgtaattg atttttcgag atatttaaca gtatttttta ttgtgtaatc
    10441 ctccttatga gtagcaacgt aagataggtg attgacaact gattcaaatc ctctatcttt
    10501 ggataaacga tcataaagcg gataaacttt attctgaagt cggctcataa gtttaagatc
    10561 gcataagtcg gcagaactga tcaaaaagtt tttgattagt aatttagtgt atttgctatt
    10621 catagctttc aagtacgatt gattcatttt ctctgtttta tcttctgcgc cttttaaata
    10681 agcagggtct ctttgttctt gcttataagg taccggaact gacatggtac gtgtaagcaa
    10741 gaactttatg tttgtataag tatgaccctt cttgataagc tcatagtcaa ttttaaaatc
    10801 agtgtattta ttcagttggg caatcggttc tttaattaca cgtctttcaa agtctcgaaa
    10861 agctttatat tttttctcaa tactaaattt ctttcgtaaa tctgcaatgg agaaaacagg
    10921 attttttaat ctatcaagtg attttgtact ccatgttaag ttatctctat ttgcttcata
    10981 cagattaaaa tctttagtga ataattgaaa aaaactgatc gcatacttgc ttgtcaagtt
    11041 aactacttga tcaaatgtat attgcgtaaa atgattttct aagttcgtta ataaaggcat
    11101 aattgaacta ctaaatttaa tagatactgc atcagaaaaa tcattgaatt cagtaatttc
    11161 tagcggtgag atgactttgt attcagtctt accgttaggt ttagttcttt gaattttaat
    11221 gacagattgc tgatgcaatt tttcaataac ttgttttaat cttgaatgtt ttgaagaaga
    11281 tgaactagaa gacatcaacg caaataaagc ctttttagat actttgacgg tattattaaa
    11341 aggcggattt ttagggtcaa gatgtcctat agcgtaaaga taaatttttt gtggcatagc
    11401 atccatttca acaatgctat ctacaagttg attcgataaa gtaacagtta aatcaaactt
    11461 ttttagattg ctttcggtag tttcaaattt cgggtttaca ttcttttctg ttattttcat
    11521 acgattgcct cttctttcta agcttggtac gagcttaaaa acttggaact ggtacgattc
    11581 cattgaataa gtatagacct gttttcgata cgttacaata agaaatgtat cgtaaattta
    11641 taacataact caatgtcagt aaaacacatt ttattttttc caatcaaatt tcattgaaaa
    11701 tacagcagaa atcgctattt tagaacattt tcaaaataaa aatccttttc aataaaatac
    11761 gaaattataa aaattaccaa aattaaaaaa caaatatttt tctagttttt agaaataaat
    11821 tcacatactt tttctttttt tggatatatt cggaatacca catcgctttt tgtccacgtt
    11881 tgcatcgctt tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc tatttaagat
    11941 tgatataaag cctttttgga ggttgctaaa gttttaaaga ttaaaatata tattataaat
    12001 ataaataaaa ggggaagctg aagcttcctc cttttccttt ttttcaatta ttcaaagtta
    12061 cacaagcaaa ataaactcct agatagtgtt agtttttttt aagatttttt atttttaaga
    12121 ttttgaaata agagaggagt aatcctcctt tattaaatat ctgttaatat atctgttaaa
    12181 tatctgttaa tatatctgtt aggggaggct taatctcttg ggagagtaag tattaaaggg
    12241 gtataaaggt aacacttttg gacagtaaag gtaacaaaag tggacagtaa gggtaacagt
    12301 tttggacagc aaaggtaaca aaagtggaca tttgaggtat cacactagga taagacgtta
    12361 ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa ttagattagt aacaaatctt
    12421 tgttgactgt tacctttagt tgtgatttaa tttaggtaac aagcagaaaa aattgtttcc
    12481 tttaattagt accagattaa catgattaaa ggagcgcaaa aagaagattg gagagtaatc
    12541 aatgtctcaa gaagttaccg aaaagattat tgatagtgat gtagatttag tatcagaaca
    12601 aacaaatgca attgtgcgtt ataacaatgg tctgaataca acagctttgc gaggatttaa
    12661 gcctgttgaa atggatgtgt tttgggctat tttatcaaaa atgaaacgtc aaggaacagc
    12721 agaagtaact tttgatttca acctctttcg ttatttggtc aaatacgaca gaagagaaaa
    12781 ggacagtttt tattctgtgt taaaagattt gagtgacaaa ctaggtaatt taacctaccg
    12841 ttttgaagat tcagacgtct atgaacagct gtggcttttt caaaaattcc ggatagataa
    12901 acagaaggaa acagttacaa ttaaggcaaa tgaggaattt gaatttatct taaactcaat
    12961 tggcaaaaac tttactcgtt ttgaacttga taattttaca aaactaagaa gtagctacac
    13021 aaaagaattg tatcgtcatc tcatgcaata cagaaataaa gagtggcgaa atggttattg
    13081 ggtagtcaaa gttgaagatt ttagaagagc attaagtata ccttcaagtt atagaatgac
    13141 aaatattgat aaaagaatac tagagcaggc gaaagaagaa tttttagctc ctgatgaaaa
    13201 cggaatacag gtatttgaaa cttttgatta tgaaaaaatc tatgcaaaga aaggaagaag
    13261 agttgatcgg ctcagattca cttttagtga gccagaatca aacttgccaa ccatttcaat
    13321 gcataattgg ttagaagaag attgagaaga agcaggttgt aattttaata caacctgctt
    13381 ctttaaaaaa gtatgggtaa cacttttgga caataaatgc aagaagtttt tttgtaagca
    13441 agtttcagtc taaataatat ttgaaagaat ggctaatcta tacaaatttt aacaactatg
    13501 ggtaacactt ttggacaagt caagaaaaaa aacaattttt cttaatttgt tgatcgacta
    13561 aaggtaacac ttttggacaa cacagaataa aaagtccggt gatatcagtt ttggtaggaa
    13621 acatcttaat atctttgtga gattgaaggt aacatttttg gacaagtcaa ctatttgtta
    13681 aaagttaata tagagtaatt agataagttt tttcttttcg tctgataaat ggataatatt
    13741 agttatttta ttgctaatta gtctttcatg atgactgaaa tagctaaaat aaatatctcg
    13801 ttggataatt gctgatactt taagggatat ctaaatttgc tgattgattt atttatgtta
    13861 ttatgatatt ataataacat aaatgtgtta cgcccgaacc tcattttaaa aaaatgaagg
    13921 ggtgagacaa tgaatgctat taattggcat gaatttgtta actatatgga gagcttacta
    13981 gatgatctaa aaagttttag tattgctgat gcagtaaagc atgggttacc agttagtaca
    14041 acgatgaaat tacgaggatt gaaaattcca gatgaaatta ttaagctgag aactaaaaca
    14101 attattgaaa ccgcaaaagt cgttgacaat atgaaccatg aactaaaagg caagaaaaca
    14161 acttacgaga aagattttga acgtttttta aaatctgtta ttggaaaata tctaggtaaa
    14221 aaatctttca gtgaattgag agatgaagag attgtaccta ctccaatggt tagcaaaatc
    14281 cgaaaaaaca aagccgaggg gaattactat cttggtacaa tagcaaaaat cattgattat
    14341 ttggaaaata aagagcaaaa gaaaggaacg aagaaaggaa gataattttt gtgttaaata
    14401 ttaatgggga agaatatacc gaaatgattc aaaatcccga acctagaatt accgaaaaaa
    14461 cgtctgattg tttgctcttt tatgaaaggg ggcagacaaa ctaatggaaa aaaacgtcaa
    14521 ttcaacatct accaaagatc aatacagtat tattggaggg ttgcttggtg ataaggataa
    14581 gagaacacgt cacggattac gcattatgct tgcaactttt ctaggagtgc tttttttatt
    14641 aacaggcttg ggtagctaca tttatcaatt aggaaactat aattattatc tcgtgaacaa
    14701 tacaacacct ataggctctt cattggagtt tgctcgatca ggagcagaga tcactatttc
    14761 agatgtgtgg acagatcaaa acagagatgt aacagtagta aaactcggtt actcgcaaaa
    14821 cgctagaaag ttgttatcta cgcaaggaaa aaactacaac ttatatatgg caacgaaaga
    14881 aaaagatcgt cctaaaaagc ttgaaatgag ctatgggatg ttaagtactg atggggatgc
    14941 tttcttattc ctaaaaggta aattagatga aagagcttat caagttttta ttgcaaacca
    15001 agtatcacta atttctggtg atgatacagc tgacgatgat accaaagtca atgtgaatga
    15061 agcagattca gttacaaaag cactttctca atattctatg aatgatgtta ataatgaagg
    15121 agtaatttct tttgggaaaa aagcaaaacc tgttaatact cctgataata tcaatttcag
    15181 aataaatcca tattcagatt caactaatgt ctatcaagga agctttctta ccaaagatgg
    15241 cgatattgat tattcaaaag ttgtagcagt tacaagtaag aataatgttg taagtaagtt
    15301 agaagatcag ttaaatgctt caaatactaa catcaagaaa attgaagcta caaaagctga
    15361 gtatctggaa cgtttacaac aaaatcctga cgataaagat tcgcaagaaa gtattcgtca
    15421 actagacagt cgaatggaga ccgaaattaa agcaatcaat gatacacaaa aattgattga
    15481 tcaatacaaa aatgctgact ttaacaaaga ttcttttgga gacatgaaga cgacatttaa
    15541 gtacctaact ctgactgact agaagtaaat tattcttatt ttagaggaag tgaacacaaa
    15601 cagatggaaa atagtaccga gttttttaag aatattcaaa ctgaatttaa tcatgctctc
    15661 acaccaatga aaccaccgaa acataagtta gtgggacgtg atgacgaact ccatgagttg
    15721 tcactgatct tgaatagacc ggaaacaccg caagctatct tgttaggtga agccggaaca
    15781 ggtaaaacag cacttgcaag tgaatgggct gaaagacagc gacaagatgg aaagaaagta
    15841 caagtataca aattatcaat cggacaattg cgtgttgggg gagatgatgt cctccaaaca
    15901 cgtttagaaa cattaattcc aagattgaaa gaatatgagc gaattttaaa atctgaggac
    15961 aaagaaaatg aatttgtcct cttcattgac gagtttcata aagttgttag ctcttttggt
    16021 agtggcaata aaattggggg tgattcgcta aaagaaccgt tagcaaatga atcacttcaa
    16081 ttgattaccg ctactacaag aaaagaatat gaaaactata tcaaaagaga tgatgcctta
    16141 gggagacggt tacaaccgtt agaattgaat gaactaaaaa gcgatgttgt cgtcgatatt
    16201 ttgagacaat ggctaaatac atcctctgtt aaagatgatc ctcaggcttc ttttaaatta
    16261 tcgcaaagga ttcctgatga attacttcaa gaaatcgtac aagcaaacaa gctttatcgt
    16321 gaggatttgt ccgaaccggc taaatcaatt gccgtaatgg aggatttgat tgcaaaagat
    16381 cgagtagacg gaaatgctta taatcgagaa agtctattgc aaatatttgg taagtataat
    16441 attcagctag attctgattt tgatatggaa aaacttcaag aggaattaaa gtccataaaa
    16501 gggcaaccgt tagcagttta tactataaca atgttgttta aacgtatgaa gcttggattt
    16561 gatcgtttta aaggtcgaaa gcaaccaaaa gcaactgctc tgtttacagg atcaacaggg
    16621 gtcggtaaaa ctgcaatggc taaagccgtg gcaagaggac tttatggcga tgaaaataaa
    16681 gttctcttag ttaatatgcc ggactacaaa acaaaagatg atgcaatgcg ttttagacga
    16741 ctcataggta ctcatgtaag gcatgatcct tctgcgatta ttgtgtgtga tgaatatgag
    16801 aaagctggtg tagaaacact cgatgccttt ttatcaattt tagatgaagg taggataagt
    16861 tttactgaaa gaggatggga tgatcgagaa gttcacgaca ctgtttcatt gtcaaataca
    16921 attttatttg cgacaacaaa tgcaggtgcg gagatgttcg attcgttaaa cagatatagc
    16981 tcactatctc gtgttacaaa caaggaaaat tctaatgaat atagcactga actaaaaaga
    17041 gaataccgaa aagtcgcatc agggatcaaa caagccttga ttggtgaagg tctaaagcct
    17101 gaagttatta accgatttgg acgaatagtt ccattttggg ctttatccga acaaactctt
    17161 ctggaaattg cttataatga aatgttgaag agtgctgaat tagtacgaaa acatttaaat
    17221 atagatttac gtcttccacc taggaaagaa agagtatatg gtcaaaattt ttatgcagac
    17281 gatctctcca tgcacttagt ttttgacatg gcttcacaag aggattctaa atcagggggt
    17341 gcaaggaata tcttgcattt agttgaatct gagtttgaag aagcgattac tgaagcaata
    17401 attacttatc caaacgcaaa aatattcgat gttaagttga gtgaagccgg agcgtttgaa
    17461 ggaaaaggtg attctaatgc tgaaattatt gcaaagcctt ccgttgctga aagtaaagaa
    17521 aacaagattg tattttcgat tggaggggcg tagctatgaa attcaagaaa tatttactac
    17581 aaaaccttag aataaaacga tgggtcgtaa gaggtctagt tattggagga gtattagcag
    17641 ttactttagg cgctcctttt ggaatgaatg atccaactcc tgacaatcaa gatagcaaac
    17701 aagaaacagt tataaataag aacgttgcat atgcggaaag taaaccggca gaacaaggaa
    17761 tgtggacact atatgctcgt atcctaatgg gagataaaga agttaatgat agtgccgcta
    17821 aaactggaca aatgaatttg ttaggacaag gtggtatcag tgccgatttc ccttattctg
    17881 acatttctaa acaaggtaat tcgattgcag ggggtaatgc aggtactcaa ctagcatcta
    17941 ctttagcaac ttatagttac tacggatata ttgaaacagt ctcaggaaat gctattgcca
    18001 gtcaagcatc atccattcta ggaagtattg gtaggacgat tggtagtttt atcggatcaa
    18061 ttagtttaat cctttctaat attgctgaat ccataaatga actccttgga aaatttttca
    18121 tgagtatgaa tatattttct cttatggggt ggggagaagg tggatcggat gtatctaatc
    18181 cggtatctaa agcattaact agcttgctta acaatttggg attaaataat gaattattta
    18241 aagcgatagc aagcttatct atgctctttt tcacagcaat actaggttat caaggaatga
    18301 aattattgtc gaaagatggt gttaactcga agggattatc accagtactt gaaaaatgga
    18361 ttgttcgtat ttttgtattg tttatattct taccagtggc aggatcgcta ttgggagttt
    18421 atatcaacgg catccagtca gcaaaaaaag atggagatat accaacaaat gttgtttctg
    18481 actacatcat taacacaaga ctttgggctt ctactcaaaa tcttgctcca tcatctgata
    18541 ctggtggtga tgtacctgac ggaaagccta catccgaaat caagttcgtt gatacagatt
    18601 atgaaccatc taaaaaatct gcaatgataa gagcgatcaa tactagcagt tatgccagac
    18661 aaaataatga tctagattct aattcagtcg gtatggctct attaaaagaa tggtcatcga
    18721 atgatacttt caatattaat acttatgccg gtgatgttaa tgtcgccgaa aatgatgctt
    18781 atatgggaag agcgcctgct gtttggacag gctataattt aggggatgga aaagaaggaa
    18841 gtgcaattaa gaaaccaacc aatttaaatg gatatatttg gtcagcaaat caaaatgtga
    18901 cagacgacaa tcgcaagcct gatggaaaag gaacagcgca taactttgat gctactcaac
    18961 cttggggcgt tgctaacaat gcaactttta gcacacagtc ggtagcttta ttgctccaat
    19021 cggcacttga tacatcggga gctaagtttt atgcgtataa tatcgctcct tcaggaattc
    19081 aaggcagtgc taagaatatg tcaacagtaa aaacagaatg gcgtacagca tccttagttg
    19141 gtcatggagc tatgggtaag tttggctcgt ggctagcgat gaacgctgaa tcaatcgttt
    19201 tagcagttct ttacttagca tgtgcttggg cgatgttcaa tgtgaatatc gtgaaagcat
    19261 ttaaacgtga atttattcgg atttgggaag tagttctaac aggatcactt gcatcaggag
    19321 caggtgtgat ggcaatttca ttaggtgttg ttacaacatc tctgatcgga ctacaattac
    19381 ctatctttct gattactttt attcgtgcat ttggtgatac tgctgtggga ttcattccta
    19441 atacattagt tggcgatggc gtagcaaata ttgttcgatc tgctatgatt ttaggtgtag
    19501 gatatctttt agtctttaaa aaaggcgaaa aagtaaataa tttgattaat atgcttattg
    19561 gcttaccact tgaagtagcg tttgggtttg atgaacgtgc taagttatta tttggtaata
    19621 gaaatgatgc tgtgcgagtt gtggacggcg tttctaaagc aatcaagaaa agccaaggag
    19681 ctaaaaatcc ctattatgat ggtggcaaca gctcagtaaa agatatggtt gcaggaggtc
    19741 ttggagcagt tggagcgcaa aaaatggctg aacgtttgaa caatatggga gatagcaatg
    19801 ctgatgctaa gtccattgct gataaagtaa ttcctgaaac accagaagga atgtcattca
    19861 acggtggcgg agttggttcc acaatcaata atacctcttt caaagattta gttcgtcaag
    19921 atcaactacg taataactcc caacaatata ttgatcattt aggacgtatg gttgatgaaa
    19981 acggaaactt tgtcgatgaa aatggtgaaa tattaaaaga tagtaaatac ggtaaagatg
    20041 ctaaaggcaa ctttattgac gaaaaagggc gtttggtcga tgaagatgga aaccttatag
    20101 atactaatgg taaaccattg gaagatagtg aagatggtaa tattggtatg gatagcgaag
    20161 gacgtttaat tgatgaaaaa ggtcgcttag tcgatgaaga tggaaatctt gttgacgaaa
    20221 atggacgcag gttgctaaat ggtgattttg gtcatgatag taacggacgt ttagttgacg
    20281 aaaaaggtcg tttagttgat gaagatggaa atcttgttga tgagtttggt aataaacttg
    20341 gtaaacacag cgatggatat aatgtgaatg gagatttagt cgatgaggat gggaatatca
    20401 ttgagacgag agatgcgctt gtggcagatc gaaatagaga actcgaacaa caacggcata
    20461 cgccaattgc tgttgcaatt cctgagaatg aagaagacga gattacttta gatcaaactc
    20521 atcaagaact gaggaatgaa atgagtggtg aggacgtgtc agacagagtg catcgggaat
    20581 taggagatgg agcatctggt gaaagtcagt caagagagct taacagcaac tttgataata
    20641 gtgctattcg gaatgacaca gtacttaaag acagagaatc gctaacaaaa gaaacggata
    20701 aagaacaatc agatgtgaag aaaaagttca ttcctactcc taatgttaat caaacagata
    20761 aatatgaaac aaatattgga tcaggaatgt cgaaagacaa tcaatcaatt agagaacctg
    20821 aactgacaaa taaaaataat agaacaaata attctaaatc tacttcagat aattcaggga
    20881 aatctaatca atcaaccaag aaaacatcaa cagctaaatc agccgttgaa tatctatcaa
    20941 aaactggtaa aaatgtttac acctcagcag ataaagcttt agcaaaagct ccaaaaggta
    21001 gtctagcagg gataacctat gaagctaaaa acgaagctat taaaataggt aaaggaacgg
    21061 ctaaggttgc caaggctact gctacaggag catccaaaac tggtaaagta gtgaagaaat
    21121 ctaccatgtc agtcaaggac tatattgtaa aatacggtaa tcaaaaatca actgaaaata
    21181 agtctgaaga cgagtaaaaa gaaggagcat tgatttgctc cttcttttta ctttgatttc
    21241 taactgaaga tgtcaaggaa aatttctatt tatgcaaaaa aattatttta aattgtctat
    21301 aaatgaatta aatatttact gttcaaagtt gtataattaa aaagggctta tgctggtaaa
    21361 gaacagaatt attatctaaa caaaaacttg ataaaccagc atttttagcc aataaaaaaa
    21421 gtgaaataat ttaatggaaa atctctttta atcatcgaaa atttatgtta ttatgataac
    21481 ataaagatgt aaatatcgag acgtcttttt cttagttaga tcattgacaa gtgaacaaat
    21541 aagatttgtc gatgatataa ttaacttatt aaaaatatta aaaaggaggt ttttatatgt
    21601 tcgattctat taaaaatttg gttagcggaa tgacgcctga tatgaaaact ttagtacaag
    21661 ttgttttgta tttcttggca ttcgtttttt cgttgaaacc aggattcact gcaatgaaat
    21721 tttttggtga tgcaaaatgg ggagatggat taaaatatgt tggggcgatt attgcaatct
    21781 ttattattcc tgtaattaca accgttatgc tttacacaat gggtactaaa acaggtaatg
    21841 atttgaacca aaaagctaat atcatatcag cacttattcc agttgttggt atctatctag
    21901 tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg aattgaacaa atttggataa
    21961 tatgccgatt tgttcaattt actttttacc aaaaaactac gctccaagcg tagtttagaa
    22021 gtgagggaag aaagtaaatg taagatggtt tttgatagag aaaaaatgtt agctcacgcc
    22081 aacgaagtat taatgagcag tctgaaaggt accgaactgg caaagataat gaatatgaat
    22141 gttaaccagt tctatgatta tcgaaatggg agtaaaaaga ttgaaaaagc cagacttgaa
    22201 acgcttatta aattcgaaaa agcatatgta tatatgctag ataaacagaa aagaactatt
    22261 gactgaaaaa aggagtttta caataatggc aaaattcaaa gatattaccg aatctattaa
    22321 ttatgtggca gaaacagttc ctgtagttgc actctctttg aagccacaat tactcgaagt
    22381 gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct aaagcaaagg tatggaaaga
    22441 attcgatgcg ttgctgacca aagatttacc aaagaaaaat tatatggtaa ttgaagatgt
    22501 acaaaagctc aacagtgagg gagttataga tgaggatgat gctattctcg attctgagaa
    22561 gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct gatctaccag aaaaatttaa
    22621 agagaagcta ttagcaggtg tccgttttat ggatagtagt ggtcaattat tagttattcc
    22681 ggatgatcct aatgtgtttg aatcagaagt tgaagaagct acagacttct atagcgcagt
    22741 agagcaatta gaagtagcta ctaatggatt agaacgaatt gctgagacct tcaactcttc
    22801 tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca gatgaaaaag aagctctttt
    22861 tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa gtaagcatag aagaaacaca
    22921 aatacaaaca gaaacctacc aacaaccaaa aattgatgaa aaacccgaat cccaaagtga
    22981 caaagaaaat tatgtttatg aagctgaaaa aatcacatct gaaagtgatg taccgaaaaa
    23041 cttagatgct gattttttag aaaaaaattt tgaacagatc attgctgaca aatttcaacc
    23101 tgtagtttta gatgagatta atgaccctgt cgatttaaac gaggagatgc tatccatacc
    23161 tgagttgaaa ccattgctgt ttttagctaa agagagtatg aacgctcgca tctctattgc
    23221 taataataaa atcaacagtg aattaaataa gacgaaggag caattgatcc tcaaaggaaa
    23281 gcaaatgtta gctgatgagc ttttgcggat agaggaagca acctcattaa atggtcaaaa
    23341 tcagtttaca aatacactat cggttgctaa agagagctac gaagcagaag catctaaaat
    23401 tgatgattta gtgatggaaa aatccaatat cattcgtaaa gattatgata tggcgaaaca
    23461 ggcttatttg gatgctgaac taaagaaact agagcaacgg tatgatgctg agcatttacc
    23521 agaagttaaa caacaagaag atgaatatag aaaagcaact gaagaaagtc ttgatcaaca
    23581 gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg aaacaaagcc aagaagcaaa
    23641 agaagttgtg gtggaacgaa ttatcaattc attgaaacca gaaattgaaa aatcattaac
    23701 agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca aagtcagaaa atgaagctga
    23761 gttccatgat acaaaagaga aaacaacaga tgtggttgtc aaatattttg cgaacaaaga
    23821 agaagttgaa aagcaggtag ctaacaacac gaaagaatta gaaacaaaac ttgtagagca
    23881 acaaaaaact atcgaagaat ataaagatcg aatgaaagag gtcaaagatt tagcagaggg
    23941 ttataaatta aatagcgaaa agttggcaga acaaagtgaa gctttaagag aagacttatc
    24001 tcgttcccga agtgatctag ctcaaatggc agaaaaaacg aaatcattag cagttgctac
    24061 agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa aagatggcta ctatcatttc
    24121 tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc tactttatta gtcataataa
    24181 tcaacaagca caggctcaaa ctcaaacatc tatttcgaga gtggaaaatt cgattgggac
    24241 aaatacgaac tctgcttcaa caaatagtag ctatgaagca acaccaacta cagagttcaa
    24301 agttgggaaa gatgttgtca tcagtattga tgggaaaaca actaaggctg aagtgatttc
    24361 tattgatgga gaaaaagcag ttgtgaaaac tccagacgga aatcagtaca aaattcaagc
    24421 tcacttatct gataataact aaggagggct attatgcgag aagaaaaaat agtttatcca
    24481 gtaattcaaa acaatcaaga aaacatgacc tcttctcagc acttacagga agcacagctc
    24541 gtgtatcagg taaatgaact accacaaacg aacgagacaa tgagcaagtc aatatcttta
    24601 attggcttgc ttattgttgc aatagtgata actgtaggtg tttaccgtaa gttgaacgtg
    24661 aaaggaagta tttctgatgg cagataactc aaactatgga tttaaacata acttacatca
    24721 aaaaccttct gaaacaattg tcagagttga atggcttgaa aagcatgtca atagcaccta
    24781 ttctgttaca gcaaaaagat taattgtatc agctatagtt gttgcagtcg ttgttcttgg
    24841 atcaattgtt tacttagttt ctgatacgat taaactgaaa tttatttttg ttccaacgtt
    24901 attaatgata attggatttc ttattctaat tagccagtat atgtttattg atccatctag
    24961 tggttcattg atttctggtt ctattagact gttcaaatca gtagttaatt cagcaagagt
    25021 atctatgggg aaaaagaaac catttcgagg cacagagatt ttaaaagtga aacaagatgg
    25081 aacaatcgta tatcgagatg ggagtgtagc attaatgttc aatctagatg gggctacatc
    25141 actaaccgct tttccagaag aagttatggc tcaggaatcg aaatcaatac aatatcaaaa
    25201 taccagagat cgttccttag ctgaaatcaa aatcacttca tctcaacctc agaatacgga
    25261 aagacaaact cgaaatatga aaatacaaaa acgaaatgca aggagtaaca aagcagtctt
    25321 tgacataata gatcagcaag aacgatttgt atcagaatat attgatggtg tgaaaacaac
    25381 agtaatccaa tatcttatct tgaaatctca atctaagatg gtattggatg aaggtgtgga
    25441 acgtttagaa aactatacga gtagaggact ttattatagt gtcactcagt tgtcagctaa
    25501 acaaacaaaa gaaatattat ctgatattta tgcactgaaa tagaggtgag agattaaatg
    25561 gaaattatgg gtagatcaca actattaaat ttcaaaaaaa agagaaaaac taaaaaagaa
    25621 caaaaaacta gaaattcaaa ggtagtgaat aatacctttg ttagtgattt aacatcttta
    25681 gttggttatc aatttacacc atcttacatt aggacaggta agcggtgggg aacaattgtg
    25741 aaattagtga atacttatgg aatgaataga aattatggat ttggttggat ggttaaagct
    25801 attcctgaag tgagagtaga aggggtaaaa gcctatctaa ttgaagcaga taagccagtg
    25861 tcacaaaaag atcaacaaga aataatgcac gagaatgttg ggggatcaac gaatagttat
    25921 gaaaataatt tagaaggaaa aaattcagat gctgaaatta gcaaaactgt tatgcggatt
    25981 atggatttga atacagcatc tttactagac agcatggcaa ataaaattat tgattggaag
    26041 ctagaaatcc tattagtatc cgattctcct aataaaatca gcgaacagct aattaaactc
    26101 caacggttgt atgatgaaaa aatgtatgga ttaaagctta tgtcatatgg aggagatcaa
    26161 cgtgatctgt ttgatagact tttaagtgaa ccaatcggga atgtctatga gtatacgtct
    26221 atgagttcgg attttgcagg aaatgatcat gcaattcgca aaggtttaga tgatgcgaat
    26281 ggatgggcta tcggcactat gtctactagc tacgcaagtg gtgtagcttt tatggcactg
    26341 gatgattctt ttacaagtaa agttttagtg gcatcacaca aaaacgctac aattaaaggc
    26401 tatgatgaaa aaatctcagc atcatcactt tgggggcaat tgattgctaa taatgcaatg
    26461 gcaaatcata aacgaacttt tcatattgtc ttaaataaat ttgattactt tggtgatcca
    26521 actggtagaa catttgttgc caaaccttcg ttaaacagtg taatagaaaa ggttaatttg
    26581 gctgaagaag gtcttaactc tttggagctt tttggtaatc aaaaggacat tggtgattta
    26641 tatagacgga atctcgataa actaactcag attttttatt tgttagcagg cagaacatta
    26701 gattctgcaa ctagagtttt attgaaaaaa gaattaggta acttttatat aagtcagaaa
    26761 ctttgggata ttaattatga agaacgttca aaacttttgg tgaacagtag tgatacaatg
    26821 ccagttcttg gggattttat tagctatttg actaatgctg aaacaaaagc caaaaaatct
    26881 ggtactgaaa aagaagtcga tcgtattatt gtattaaaag acacattgag aacagcatta
    26941 gacagcttct ctaggatgtt taatcaaaga acgacactga aagaaatcgg taaaagtgac
    27001 aaattacagt tttactataa cttaggtgat ctaagagcag aaaccaatgt tatggaagct
    27061 caatttttaa atacctttga tttcatcaca tcacaagcaa aaaaagatga tattgttatg
    27121 attcacggct tcgataagtt atctattgaa accgttgggc taattaaaaa aagattaaca
    27181 atgcttgaag aagatggaat tcgtcttgcc tttctatttg atacaatcgg ttcaggagaa
    27241 gacaatgaat acaaagaaga tattgaaaca gctgatatat tcaatacaga tggaaaaatt
    27301 taccaaagtt ttgaaagtga ttttaattac actatcattg gaaagatgac cagtggagag
    27361 ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag gagaagatga tcaaaaactt
    27421 tctgttgatt tgagacagta tttgacaaca ggaagaagta ataaatttca gatcaggaga
    27481 ccagtggatc tgactaataa ttttgttatt ggagagttta ttctttaagt tttatctttg
    27541 attttgagtg tataaggaaa ggataagaaa aaacatgtat atttgagact aaaaacagtc
    27601 ttagagaaca tggtacgttt tgtgaaaaga gaactataga aatttcataa agtgaaacct
    27661 tattgagtga aataattata gtggaggtta aataatgaaa ccaaacataa aaattttaga
    27721 tcgaatattt ctaggtagag acacagaagt aatcctaatt caacatgaag aaggatttga
    27781 agttagtata ggtattcaga aacttcaaaa acctcattat tgtaatcagt tgtataaaaa
    27841 ctttactgat gaagaaaaag cgagagtttt ttttaatgaa gtcaaaggta tgagagaaca
    27901 gtatgaggtg gttgaagcat gaataagtta ccagaaaaga accgtgtatt tagattgaaa
    27961 ggtgatctca gtcaatttaa aatagatgag aatggacaat tttttgtttg gatgcgacat
    28021 gagcattgtt ggtgtaatcc ttttgtgtta aaaaaagaaa atgaaatcag aactaaaatt
    28081 gcaaatggtg agtgggatta cctcttcaga tgggaagatg aatcttttat agcaaatgat
    28141 atggatcatg catttgtctc atttctggat aatacatggg gtacttggta cgattgttac
    28201 ctagaaaaat tgggctttca aaatattgat attgatgcta ttgaatgtct taaagaagtc
    28261 gatcaaaagt atgaagaaga accctattat ctcttgcaac aaaaattagc caataattca
    28321 gtgtttcagg tagaaatttg ggaaaatgaa gatggaactt atgaggggct agcgcaagaa
    28381 atacccttct cagataggaa gagttcgatt tgaaaagagt atatcagcaa tttttatgaa
    28441 gtgaaagcaa gcttacaata tagtaatgga aaattgtggt tagctggaag gtatattcca
    28501 gctaaccaaa tcttacgttt tctgggagag tggcgtttta aaacaatttc ctaggaaaaa
    28561 tagttaacat aaaaggaatg aattgttatg aaacaatatt taaatgaatg gaaagcgata
    28621 gaggggtcat tagtaacgca aagaataaag gaattacctg attgcttaga gaaggatcat
    28681 ttgtttcaaa tacgagaaat gttgaaaaaa gaacagtttg atccagacca atttttagta
    28741 gtcgagtatc caactattgg agtgtactgt tgtaaccata tcaatgatgg aaaatatttt
    28801 attattcagg agtatggagg acagttaact cctttctata ctacatggga aatgagtgaa
    28861 gaaggaatca ataattttcc atgtgaaagc atcgaagatt caatttcaca caccgaatgt
    28921 taattgatta taactataaa aaaggattga acattatgtt caatcctttt ttcaatgaaa
    28981 tgttttccgc catttcattg tttggtgcga tttctatttt attatatcaa aataaagaga
    29041 gattctaaag aaagaagagg gaaatatata aatcaagcga cagtatattt attattaaat
    29101 gctattgtga gaatataact aattagttta atttgaaagt aaatatacgc tataaaagtg
    29161 catcttgtgt tataaaaaat tattttattt tgtgaacata atttaacaat tctttctcgg
    29221 aattgatttc ttttcttaaa tagtcttcag acttattaat aatattatct attatgtcta
    29281 gatacatatc acttttcgat tcagctaatt catacagttt ttttaattct ttatccttta
    29341 attgaaaacc ctgatccata gtcactgcat ttggatatag aagcccacta taatgtttac
    29401 gtaaattcac ttggaatttc tgattataaa agtctaacag attagaaata aaaggcggaa
    29461 aattactgtt tttgtaaaat tgattatttc catttaggtt gtatagtaat ttgttaaatt
    29521 gctcctgatt taatttattg taaatccatg gtaatacttc catatcctta tctgctaacc
    29581 tgaatgaact agcatcggta agatgttcga tcataaaatc tatgaacatc tctttttcac
    29641 caaaatataa atattgatta tatattgttt ctaatgttga agatggtagc gaagaaactc
    29701 tcattttttg ctggagtata ggatcaacta gactatttaa gtgattttgt aattcttcat
    29761 tgttgtttcc tagaactgca agggaacgta aacggtagta atattctatt tctgttagta
    29821 cttgttgttt cggtttaaaa tcatttattg atatttgtaa attttgaatt gtctcgtctg
    29881 aaaaatcatg ttgtaattct ggatgggtag ctataaaagt aagtaaaaag aacccttttg
    29941 tatacgtgat ctgttgattt tcaccaagtg atttattaat ttcctctctt gattcagctg
    30001 tgaacctata ctttgcaatt tcttttttta gaatatttaa atagaattct ttattatcaa
    30061 aaattagggc attaagtagt ctgaaagtac tttccctatt tctatctgct tcctcatttc
    30121 cataagataa ttgaaaaaca aacttaaagg ctgtcttaca taggttttct ttggcttttt
    30181 cgcttagttt atttagatag ctatttttta gtgcagagtg aaattcttca ggtgtaacag
    30241 acatagcatg tatgcgactt gcatattgta taatttcgat cgttactttt tttcctaata
    30301 ttggattttt taggaatata atttcatagg acttccgtat aacatcctga caagtttctt
    30361 ttgttatatc tcttaattcc caagtattac cgttgtaagt gactacagga tgagctgaat
    30421 aatttctttg atccttaata tattttaaat cctcatattc atagctatca attagctcag
    30481 ttgaactata agctttttct agtagttcca tttcccattt tggagagttt ggatcacttt
    30541 ttcttagtct agctatatct tctaaaattt tcttagaagt attatcctcg taaatatttg
    30601 ctaaatcaga taatttatca attaaatcta aaataagcgc actgtaattg acagctattg
    30661 cagaacgata atttccctga taaaaactac ttctaacttc ttctaataat ggaataattc
    30721 taggattttt aacttttttt atttcatcat ctaatataaa catataaatc tccttttttt
    30781 atttgtatga caagtttacc ataaaaaaaa attaaaaagc gagacatatt tatgttgttg
    30841 tgttatcata ataacataaa tatgtagagt gagatgatga aaaaatggat ttgaaacaat
    30901 cgttttacat ctggttatat cctgataatc ctatgtcaat aatggctgat atttttgtag
    30961 ttagcatctt attgtattta ttttatctaa ttttctttca caatttaatc gggtacatga
    31021 ttcttgattg gaaaggaatg ttcgatatag aaagaaaacc atttgctaac aatcaaacct
    31081 ataccgtctt cgaaagtgga gattgggaag aaaatgaagt tgatgtgatc catgaaattg
    31141 gattagcttt atcaggaata caagatgctg atgttttggt atcaatgaca acggatactg
    31201 gatacaacgc ttatgaggta ttttatctta tgtattttat atggcgtgat ggtcaagaaa
    31261 ttatcaatgg tcgtgctgaa aaaacaatca attctaatac tgaaatttct ttttgtatgg
    31321 gtgaagggga aatgattgta tataaagatg ggtcagcaaa cattaaaaca gcctttggtg
    31381 atattgaaac aagtattaag gaacttaggg aatatgtgca gaacccatat aattttcaat
    31441 atttagcagg aagaataaat ttagtttagg agtaaaaata tgaaaaaaat tataaatcaa
    31501 ccaacaaatc acttgataat tgaaggtgtt attttcgtaa caatgtattt tactgtagga
    31561 aatggcatct caaatacaat cattcaaaaa aatccacaat acctattatt tagtctttat
    31621 gcttggattg tacaaatggt ttgggtttcc atattgatgc tgttgtttta ttggatagta
    31681 cgggttattc ttttattgtt aaacaaaaga aaaaaggata ggtgaataaa atgaaatcaa
    31741 ctaaattgaa ggttgtattg ttatcgacag cagcattaat aactcttaca gcatgtggta
    31801 caactaatag tgctgataat aacaaagaca caaagtctag tagtacggtg gttactaaaa
    31861 acagctctac atcggcatca acaacaattt cttcaatgac tgaaaataat gctgaaaagt
    31921 atcaaaaata cgaagacatt aataaaggtc aaaagccgga agaagtttat accgacttta
    31981 ttaatacagt aaaaaatgat gaagattatg ggaatgatcc attgcaaaat aaaaatctga
    32041 ctatcgttct ttacaaacct gattgtccag attgtcaaaa agcagaatca aaaatcatgc
    32101 aagaaataaa agaaggattt agttatgctc caacattaat gcatgatatt accataaata
    32161 aaccagctta cgagtatcac gcagttgctg tagatattac tgatggtgta cctgagtgga
    32221 tacaaaacat tcctggtctg gctgatgaga acaatcaata tcatacgcca acagttgcag
    32281 tagtaacacc aaagaaagtt aatggcacac tatactggga tgtaatggga atgacaacaa
    32341 atacaactga tgaaatgatt gaaagtacaa ctaaatatta tatggatttg ggagcatata
    32401 aattatatca acagtcagaa cctgttatgg acgatcaatc caaagttaaa tagttcttta
    32461 gggcgtaagc cctatttttt taaaattaag tgataacaaa taaatgattt ctgaaacaaa
    32521 cgttcttact caactactcg aaacacacaa gctaattaac tagattataa aggagaatgc
    32581 gtaatgaaag aaatcagtgc aaatgaagct catattccgc aaaagaatca ggttggtata
    32641 tataaggtgc ttcatggtga aggaatttat aaaatacagt tacttaccgg ttacaataac
    32701 ggtgcaaatg ggaagtggtt aaaggcaaaa agtattcgtg aacttaaaac tgaagtagaa
    32761 aaaataagac ttgggttaac aagggaagaa ataagtagac tgagtgaaaa ttcttctttt
    32821 gtaatccaaa aagaaaagga gaaacatcta cttcatcggg aactaataat tgaaaaatac
    32881 tacgatgttg atttacctga tcagttaaga attattgacg aattttcaga gaaaaagtgg
    32941 aaatctgaaa tatacccaag cttaattcaa tacaaggcat caggaaagcc catgtataac
    33001 tacacaggct ttttaaacta cacacaatat aaatatgcct ttcgcaaatt aaaaggcatt
    33061 aaacaagctg acattgccct gtatgagatg aaaaaaatgg atagattgaa attgattcaa
    33121 gagttagtgc aaaatacatg gaatcaagcg attgtcgaac tagaaaaaga aaatgggaaa
    33181 attttaacta atgaatatct gatcgtaaat gaaattgaag aattaattgg tttattttca
    33241 tgtgaaaatg gtgaagtctt cttgaagtca tcaccaatct attcaatttt ggacagtccg
    33301 tatcagttta tacaggtaag gagatgagtt acatgtctag cttgaaagat gactatatcc
    33361 aagcaaaaca agagtatgaa aaaatacaga tgttaaaatc tgagaaagtc gaattatttt
    33421 taacacaaaa attagcagag gatcatattg ctaacttaga tgctaaaact ttagaggaat
    33481 atgatcagca gtttaatcag acacatcaca atatcatttt gcttgttgat caggcaaaga
    33541 gaagacttga aaaagcggaa tatgaactat tagatcaagg aattatatgc ttggataaag
    33601 ggattcgaag atattatagt gcagagttaa aaaagcaaaa tgtagacaga catgcgttaa
    33661 aacatgaaat cttagaattg ataatgcaac gaatttgaag ggatagaaag taaatgtcac
    33721 aaaacattag tgctgtaaaa caattaatag aaaaagattt agagacgaca gttcagaggt
    33781 tagccgaacg aatccatttt gcaaattatc aaaccatctc taattggtca agcaggaaca
    33841 gaaatccgga agaaattcca atgaaatttt tttatcgaat cggaaaagaa tatggtgttg
    33901 atccgataaa aattttcgaa aagaccatga tttattacta tcatggaaca cctaacgaag
    33961 tgttggaaca tcttttggaa gaagcagaag aaaaatcatc aatattagag atatcacaaa
    34021 atgacaaagc tattcaagtg aaatgtgttg atccaacagg aaagattatt gttggatttg
    34081 atattgaaag aaaatctaaa atagatttta ttactcaatt aacaaagata aataatttaa
    34141 atattttaac acttagacca gatcatgaac agttgtttat gagttttaaa aaacaattga
    34201 aagaaataca aaaaagatat aacaaggtgt tttctaatgc gtgaaaagct aaaagaaatc
    34261 agagtgtttt ggataattaa caaagaattt attatcttgt atacacttgg atttatcagc
    34321 atgatcttgt tttggtggtt atatttaaaa aagtaattta aaagtgtcaa ggagattttt
    34381 atccagagaa ggatgtggaa tttgcatgtg gaataataaa ttaaaaacag gcttgttatt
    34441 aatagttatt tcctgcgcta tgatggttgg catgataata caaagggaac aatcttattt
    34501 tgaagtttct gctaacaatg taatagaaag atgccattat ggtcaacatt tttggactgg
    34561 ggaagtcaga gaaaatatag atcgtgagta tgttcaaaga atcgtttggg atgcatattc
    34621 tatcaaagat tatcctaaaa gtttaacgag tagattattt tacagcgaaa aagataatca
    34681 aaaactctcg aatttgatga tgaaaaaagt aaggaaactt gcacaaaact atttggaaga
    34741 aaaagaaggt aatagtccaa ttgacaaaaa ttgatgacac tgcaaaaaaa tatagagttc
    34801 gaattagctt ttttcggagc attgtttttt aggtgaacta tatttcatca ctaaaatcag
    34861 agataactaa cttgaaacat ggtcagatgt gttacttgaa gtctctgaat aatcaatgtt
    34921 atctagtaga attaagcaac taaaaaagaa agaagtaaga aaatatggca tacgaaaatt
    34981 tacaattagt agtcgcagaa gttagtgggg atatatacct tacaaaagta aaagatggaa
    35041 ttatggacac gaattatagg agagttgcta cagatgatgt gctaagctca tcgacagaat
    35101 ggtttcgaaa aaatgaagaa tctgctgttc actttgaagg aattgatgga aacattcata
    35161 gtttgttcta tacaaaagac aaagaaaaag caaaaaaaat tcttgatatt ttgaaaggaa
    35221 ggtagttaat taaccaatga tataagagaa tgtgagacca agtaaatact tggtctctat
    35281 aattttagcc actagtcaat taaatagcta aacaatttta ataataataa aatatgactg
    35341 cttcataatt aagaagtgca gaatttttct aagaacgata tttccttgtt gaaatgagta
    35401 ttgctaacaa tttgatcacg aacagcatcg ttacaaatgt tgtatcaata tttgatagat
    35461 tacatattct attaaaaaag aaaagaaaat tattgatatt atacacatta gtgtgtataa
    35521 taaaagagag ggagtgatta gaactttgag caattcacta attgtttaat aatagaacga
    35581 ttcctaagaa gttcattgtt cattggcaaa ttgctttatc agattgaaag ttggcgaata
    35641 aggtttgtag aataacacct tttcaagcaa agcgattttt gaaagctatg aaatttgttt
    35701 aataaaaaaa ttcaccagat aatctgatag agactaaccc aattactaac agttgtaatt
    35761 ggtgttagct tacaaattaa ttattgtgtt tagtcaatta caaattgtga aacggcacta
    35821 ataattggaa aggaaaaatg aaatgtctaa agatttacga gagcttaaat tgcgaaaaaa
    35881 gcaatataat caatattttt ctagtttgtt gaataaacat attgaagaag gacttgatca
    35941 tgattttgga aaagatgaat attgtttata ttgcgaacgt tacaatgaaa aaatgtctga
    36001 atatctacag ctgaaaaaag agatacagaa attagagaaa aaagtacatg cttggaagta
    36061 taaagattcc aggtctggtt atgttgaatc tcaaaataag ccaagaaaaa agaagcaagc
    36121 gtatgttcta aagatggtaa atggggaaga gttaattgta ttagcgtaca gtgaagaaga
    36181 ggttcgagaa ctcgttgaaa cacctgtcag gaaaaaggtt gaaacaataa atgagataga
    36241 cgacagccaa tacaatagca tctatattga aacaagtcat ggtggatata agaaattagc
    36301 aactttcctt agaagttcta aagttgggat aatttctaaa aaagacataa gtgctgttac
    36361 cgatagtttt tgtactttat ctgttcctaa agcttatttc caatacgata gtaaaagatt
    36421 tgaacgctta gagaaattct gtagaccgta tactgtattg aaaactaaaa aatttaataa
    36481 aactaaaaga gtttcgatac agttaccgac tggtcaaatc tcattaggtg aaaaagacta
    36541 tctgataaaa gtaggaaaag agttaattat aaaagtaccg gaacacttat ttaaagaaaa
    36601 ttttgagata cgtgaaacct tggtatggaa agaagaaaaa tagtttggaa gacatagaat
    36661 ggtataaggg gaaattgaaa tggcagtaaa taaaatattg caggtattaa tcatagacga
    36721 atttggcgga ggagaatggt ttgatgtgca gtccatagac tttgaagaag aagtcgtagt
    36781 tatagaaaat atttacggcg aagtatactc atattcgctt gaaggtgaga atgttgagga
    36841 tctacggttg attggtgagt agaaaaaagc agaaatatca aaatggagat aaaaattaat
    36901 agcatgtaaa gatagtcatg cttacatgct ttatccaaaa tggtatgcag acaaaaatat
    36961 agctgaaata aacgattatc taactagtat aaaggaacgc aatgcaatta attaacaata
    37021 gacgaaaaat gaaattaaag gagataggaa aatggtagaa gttgaatttg gatcaaaaga
    37081 atggtttgaa gagttaaact caatgattaa gttcccagaa aagtttactt tttactacaa
    37141 taaaagagac aatatcattc aagtagcttt acttgaaact aagggaagat tagaaaatac
    37201 tcgatggagt atgataaaac catcggcact tagatttcat cctaaagact tgacagcggt
    37261 tgtagcggta aaagaggttc aagactcaca agaatattat gaagtagtaa aaaatctgaa
    37321 ggatatttgg tgtaaataat tctaaattga ccagttattt ggtcaaaaat agtatgtgat
    37381 tatttcatcc aatgtggcat cataattggt atggaagggg ataaattatt tgaaagaaat
    37441 agaaggtatt aagaaaagaa atcaggaaag attggaactg ttaaatgaaa atagaaatag
    37501 aatgtttagt tattatgaat ctatgaggtg tttaaataaa tctagaagtg aaattataga
    37561 atcatcaaag tataaacaat tgatggaaga gagagtttct ctcatccaag aaatagaaac
    37621 agagtctatt tttccagatc attctcctaa agtccgattt tataattaaa cattaacaaa
    37681 tgaaacgatg ctaaataggc atcgtttttt atctatttat aaaattaatt tatttacaaa
    37741 atgtgtatcg gtatgtatgg tgaatttgta gataagttga gaaagggtga tcttaaatga
    37801 aattagaagg tagcttacaa aatagaatga tggaaggcag tacaacaaac aaggagattg
    37861 tagttggaat gggagcaacc gagcttctat ataccgacag aaatccttat acagtaattg
    37921 aagtaaaaag caaaaacaga attttggttc aagcggatgg agcaatcaat aaagctactt
    37981 ttccagatca agagtgggaa tatagcagaa atcctgaagg tcaaatattg gagttagtta
    38041 agacaaaaaa tggatggaaa gtgcttaaag aagatattca tttttatata ggtgatagag
    38101 aaaaatacta cgatcctagt ttttaaacaa cctgaagatg agcgaggttt tatcctcgct
    38161 cagaaaggag tatggaggat gttcagtcaa gaagaaatca tcagcaaatc gcaaccggat
    38221 aaaaatggaa ctataatcat gaagaaaaaa atcccttgct atcgttgtag cggatcaggt
    38281 cagtacaagt ttatgtctat ggggaaaatt acctatggca catgttttaa gtgcgatggt
    38341 gatggatttc ttgttgtaac tgaaaaatcc tttacgccag aatatagaac taagctaaat
    38401 gttttaaata aaaaaagagc tgatcgaaaa ataaggatcg caagagagaa agaatctcag
    38461 aaaaattttg atcgttatgg ctttgaaaag ggttatattt acgtggttat agagaaaaat
    38521 agctatacga tcaaagatga actgcatcat caaggagcta cttatgatcc tttattaggt
    38581 tggttttttt aaggaaaaac aagaacaata caaaactcaa aaagtttttg tagaagaatt
    38641 ttctgtaatc aatgatgaag gaattattga gttgaagcta gaagaataca gcaaaagaca
    38701 acaggagatt ttaagtgcga agcgtagaga agagggtgag tttattggta caatcgctca
    38761 acgattggaa ttggatttga aacttaccaa taccgtcacc atagatacac aatggggcaa
    38821 tgtttacctg catttatttg aagatcaaaa ccataattca ttgatttgga agacaaataa
    38881 atttctttat tatgatgaat gggatgttgg agaaataaga aaagttaagg cgacaataaa
    38941 agcgcatgac tactacagag ataccaagca gacttggatt aaaaatgtta aaattcaaaa
    39001 aaatgagtaa gaagggataa aagtatgaaa agagaagaat acaaacaaag attaaacgaa
    39061 ctgcttgaag aggacgaaac attaactcat ggttcgccgg atgaaattct atacatgatt
    39121 gataatatgg taatttttgg tggatatgaa cttggtaata ggtcagttga tcataatatt
    39181 ttagaatttg atgatgtatc atgggaagaa attcttgatt ggggaatttt ggcagtacct
    39241 gaaactaaaa cctatattag tgacactatg gttccttttt ttgaggaatt ggattataaa
    39301 agacttccca aaaatgaaaa tcatatactg ggaggaaact aacaatgtta agtgacttac
    39361 ttattttact gatctatcta ggtggaactg aaattattgc gtattttttg cttgtaattg
    39421 gtgaatatgg tatttccagt gcaatcactc aaaggtttag attaccagta agtgaactaa
    39481 aaaaatgatg agattatcag catatctttt aatgtttttg aatgtggtca tgttaatctt
    39541 tgtagtaatc ggtgtgactt tcaatttaac ggaatagagg gaaatctata atggaaaaaa
    39601 gagaaaagaa tgaacatatt ttttgagtca atattgttga tggtttttgc gatcagcgct
    39661 taccagaaat ctaaaaaaga gtaatgactg tttagtcatc actctttttt tgtagggaaa
    39721 ttgaaatatg tggagcttgt attggtttag ttaggattgc ttggctataa ggagctagtt
    39781 ctttatcaaa accatcaaag taggttttca gtagcgattt aactgatcgc tcatcttgtt
    39841 tgatttggat aatttcagca tccaaatcgg ttgtgaggtc aggagctttt gaagcgatta
    39901 cctgattttt tttatcttcc aactttctta ggctgtttaa caagttgtaa aaataatcat
    39961 tttgactgtt cattccaacg tgagatgcaa ttttaacaga agtttcatcc gtattggcat
    40021 caagtatttc atcaaaagaa ctcattggaa tatcaagtag taacgaggat gtttccttta
    40081 cagcattttt agtttttcca tcttttttgc tcttaacttg agatattttt ggatctagtt
    40141 taggtaaatc acttgtttta tcagtcattt attagctttt cctttctatt aatttatgtt
    40201 attatattat cataataaca taaaaaagta cactttttgg gggcgtagtt attgaaaaaa
    40261 tctaagagaa agattgccac gttgcttacg ttaatggtgc agtttacagg gattgcaatg
    40321 agtgtaatca caataaatcc gcaagtgtta catgctgatg ggatagctac ttatgatgca
    40381 caatttaact gtactactga aggtagtggg tcaggaacag atagcgatgg aaacgatact
    40441 ggaagtggcg ctgtaacagg tgatgtagcc aaaaatattg aagatattta caaatacatg
    40501 aatggagaat atggcttatc aggtgctagt attgcaggta ttttaggtaa ctggaaacaa
    40561 gaatcaggta ttgatcctcg aacagtagaa gggtattacg gcgatgggca gagagaagtt
    40621 gacttcatca aacaatttac tgatggtcta atgggtattg gctatggtca atggacagcc
    40681 gggcgacata cagcattagt tgattggtct aagaaaaata atggcggttc ttggtggact
    40741 acagaagccc aattggactt tatgttcaaa ggtgatgggg gattcgtagg aatattaaaa
    40801 gattatgctt taaatgcttc tgatgatgta gtagaaaatg cagtaacttt tcataaaaac
    40861 tgggaaattt cagctgattc cgaatcagct gtaatagctg tacgtggtga aaatgcaaaa
    40921 gaaatctgga agtacatgaa aagcaagggt atggacggtg caaaagatga aagtaagatc
    40981 aataaaatat ccggaggttc aagtaccgga gatggatcga ataattcatc aagttcagca
    41041 tcaggaacag tagaagatgc ctgtgcaact acggatgatg ctaattctgg ttctggatca
    41101 attgggaaag aaggaaaaat taacggtaaa aaaggtcaag taatagcaaa tatgacttat
    41161 gaagaagcat taaaaaaata tgggaaagca atcacgctac caaaatttaa agaaccagac
    41221 tggtcaacat caccttttca aggtgaatta caaggacaat gtactgaatt aacatgggct
    41281 tatatgagtc aattatatga tggaaatcaa ccaactttgg gaaatggcgg ttacgtttgg
    41341 gaaagctaca aatcggcagg agcaaaggtg acgaacaaac caattgtagg ttatgcgttt
    41401 agtgctttag atggatggtt atgggctgtt ggatcagcag gacatacagg tgtagttata
    41461 gcggtgtttg acgatggatc atttttgtgc gctaatttca atgttccacc tacccaagca
    41521 ccaacaagag gtgtttcagt aactttgatt gatggtgtgg atgggtcaga taatgtgcat
    41581 ttctttagcg gtgtaggcaa agctaaattt gaaattgaaa aataaaaatt tgaatggagg
    41641 gcgacagaga tgaatagtaa aacgaaagat gcaattatca tttctttgct ttctggtatg
    41701 ctattagcaa tgcttgttgg aattctatat ttcaataagc agaatcaaga catagaagct
    41761 gaaaagcaca gtgtgcaaat tgaaaatgaa ttgaccttct ttaaagaggt taaaccaaat
    41821 gaagtggatg gattgcaaag agatttagaa aatgatattc aagagatgtt taaaggatca
    41881 gaccgaagca aatttgcgaa tcagtcaggt gaagaatttt tgcgaaagtt atttattgtg
    41941 acaggtaagc caccagttac taacgagagt agcgagcaag aaaaaataga atactataaa
    42001 ccttttactt ttaagctaac aaactttgga attaagacat ctagtagtgg gttgtatgaa
    42061 gcattgactg atttcgaagt tcaatacaac ggaaaaaaag tgtatgatac cacattaacc
    42121 atcacactaa ataatgataa gaaagtgact gggggtgctt tttatgcaaa atcatgaaat
    42181 tactactgct aaaaaggcga tcggaatact ttccataatt ttgatccttt gttgtgtggt
    42241 actatttcaa aagaaaaatg cttatgataa taatgtcaaa gaattggaag accttaaaag
    42301 taaacaaatc gaagtagtca agaataacca gaaagtgaaa gctatgcaag aagcccaata
    42361 caaagaattg ggaaatgctg aaatcaagga acaaagtagg aaattcttta atttatttta
    42421 caactggaat tcatgggaaa aatatacgaa gaatatgaag gaaatacaaa aagaatttcc
    42481 tcagatagta aagtctagtc ctgttgatat ctctggcaca atggtaggta ctggtaatag
    42541 tccaatatct agctatactg ctgattacta tacaacaaat caaaaaggta aaatggttga
    42601 attgattaca cagacaagaa atactccaac aacagcaaca acaacaattt ggcgtggagt
    42661 aacaagcata ggagaaaatc aattgtttta cttggaggat ttagatagct atgtaagagc
    42721 agagtaaaat aaaaaaagag ctaacggtac caacgttagc tctttgtgct gacgatgata
    42781 acaaaaccta ccgaaaaagg attgtttcat cacaaacaca aacagaaaag aattgataaa
    42841 aaacccgaac ctcagaattt ttattaatca attcattttg attataataa attgagctga
    42901 acattacaat acgtatatgt tgtaaaacgt aaaaaaagtc aaaagtaaat actttcttaa
    42961 aaagtcaaat aaaactatta aagacaatat atatggttta tttgacataa gaaaatggtc
    43021 tagatgataa ttagaatata gaattgtaga cttaaataaa tcataaaaaa ccttctatga
    43081 taagttatag ctcttaattt taagcagttt tagatataag aaagtaaagt gggggagaat
    43141 aataatggca gatcgtaaat tagaaaagtt attagaagaa acgtgggatc caaaagaatt
    43201 tagtgaattt tttatggaga attttgaaac tgacttagcg gtgattgtta aagatgctct
    43261 tagagagcaa gggtatcctg aaactgcaaa ttatataaac attaatttta ctttgtatac
    43321 tgaaaataga ggaacttggg atttttgggc taacttagca aataaggagt taagtgataa
    43381 atcagataca ggaattcgta acttttttga atcaaacaga gatgattata tgtacgctaa
    43441 caatcaaaat aaattgaact ttagagtaga gtttgatgaa acacctgaag aatttataga
    43501 aagacaaccg cctaaagaaa atgtagcaaa ggtattggaa gatcgttgga attcagatga
    43561 aatagtttca acaattagcg aacaagacgg acaatatgaa ccgctagtag aagcggttag
    43621 agaagagcta agactgaata agtttcctga tgttcaaaat attgatgttt ctcaaattga
    43681 aataaacgtc aatatcacca atagacttga ttatgatagt tgggcagata tagcattaga
    43741 aaaatatatt tactcaacgt taaaagagtt tatcgaaaat agaatgaata tcatgtatct
    43801 acagcatcca cagtatttaa actttggtgt tgaaatagct acacctcttg aagaatggaa
    43861 aatggagcaa gggcttgact agcctttgct ccattttttt attttacaaa tgaatacagt
    43921 tcttcgtctt taccaagaat gattgtttgc ccaactttga gattatcagg tgatccttca
    43981 gcggtttgta aggagaacgt tccattgaac ttatcggata ccgttatagg tagctttaat
    44041 tctgatcctt cctcatcttt gagaattgct gttttccgtt catcaaaata aacagtcttt
    44101 attagataat ttttagtagt ggtttcatca gatttgtctt tgtttttagt agtactaatt
    44161 tctttagaat tagatgtatt ttctatgact atttcgtttt tggtatttgc gcttgtattt
    44221 atttgattga aaattttaat cgacaagatc ccactaagaa tagccaacat agaaacaagt
    44281 gtaattaata ctgataattt cctttttttg ttcatttctt cttaacttcc ttttttgttt
    44341 tgattagtta gatattcctt atctaataag cctttttagg ctattaaatg aagataaacc
    44401 ccaaaaaatt attgcaataa tgatagtgca atggtactat tattataaaa taaattttgc
    44461 gaaaggggta ggtctaaatc gcaaataaaa agaaattgaa cctagacaaa tacaacttgt
    44521 ataccgcaag ggaagcaagt ctaattttgg gtaaaggtag aaattatgtt acaaacataa
    44581 tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt gacaaaacct
    44641 atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat aaaaacaaaa
    44701 agaaggaatg aatatgaaga aaaattcgct tgctatggtt ttatttgcat cagctgtact
    44761 tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa acacaccgga
    44821 tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag atactatttg
    44881 gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg agtttgccga
    44941 attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg gacgaacttt
    45001 caacatcaat aaagatacat taacagttaa ggaagcagat ggcgaagtag ttagtcaaac
    45061 tgttcaaaaa gataaacctg atcctgttga aaatacggag caagagaaca aaaaatccga
    45121 gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa caagtgcttc
    45181 aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga aagaagcatc
    45241 acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa caaataactc
    45301 acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg atcaatcaca
    45361 aacaggttca agtgatgaaa ctgacagttc tacttcacca agtaaccccg aacaaccaac
    45421 taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt gcgaaggaac
    45481 atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt taatgacttt
    45541 tgttaaatca atgccactat ctcctgataa taatccaaat aatggttttt ctggtgaatg
    45601 gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag tttcatgggg
    45661 tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag gattaaaaga
    45721 tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg aacctgctca
    45781 acataaatca atgttttggg gcgatgctat ttggaatcga catgtagtag ccgttgatgg
    45841 atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt ggattaatgc
    45901 aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac gttaataaaa
    45961 gagatcgttt atatactaag tagttaagta tagtctttat ttgtccttgc ttaactactt
    46021 agaaaacttg ttgctcatgg ttggtagaac taaaaggggt gatgctatga gtactagagc
    46081 gttgaaacag cttgctttca acgaaaaaag tgattaatag gtgtggtgat tttaacttag
    46141 gaatgggcgc gaaggtcatt accacaccga catatcgtgg ctcattttct tcgcctaact
    46201 ggtataaaga aggagtaacg agagatgaag catgaaaaaa cgataatttt aaaatcaaaa
    46261 ataatttatt gttttgcgac tttagtaatg gtgttatcta cgcttataat gcctttttca
    46321 gcaataagag ctgaagcaga tgtcacaaaa ccgacaagtg caaaatttac agaaactggc
    46381 gtaaaaacag acggttatat tagggttaat atgccgaatc atccaaatga atggatgatt
    46441 tcaagtcaat ttaaagattc tcatggaaat attggatatt gtatggattc agaattgcca
    46501 tccccaacag gaagtggagc cggaagcttg aagtacaagg gagctggcag tgatgagttc
    46561 tatcgtatgt ttaaaggtgg ttttccatca aaaacagcaa aggaactggg tgcaggaaat
    46621 gatacagaag catggtatgc aacacaatta gtttcatggg ttttagccgg aaactttaaa
    46681 gtttcacaga ttgtatggtc acatccaaac cataccgccg cagaaacagc tcgtgtaaaa
    46741 aaagcatttg aaaaaattta tgactatgct aagaatggaa aagatacgcc caacactgag
    46801 ttttctatca cagcttctaa gacagctgac gaaggaaaat atcacacttt cacatataaa
    46861 acagcatcaa ataagactgg taatgcgaag ctcactttta catcagcaaa accagctggt
    46921 atgaagattt atgatgcaga tggtaaagaa attactaaca acactgtgaa attaaattca
    46981 agttttacta ttaaagtacc tgtgactaca ccaagtggta ctttgtcatt taaaggtact
    47041 gctaatgtta gcacaaccaa tccttttact tttgatggac gaggtgtgta tcaagatgcc
    47101 gtagttatga tcactacttc agaaacaaag gactctaaat ctttgagtgc taaatggaca
    47161 agagctgttg gtcaggtaac agttaagaaa gttgatcaag atggaaatgc tttaaaagga
    47221 gcggagttta ctctaactga caataacaat gctaaatctg ttaagacaac cggagatgat
    47281 gggttagcta cgtttgattt gcaacaaggc ttaacataca gcttagaaga aacaaaagtt
    47341 cctgaaggtt atcaaggttc atttaaaaag gatggtatta ctgttaatac cgatggacaa
    47401 gaatttagtt acactgctac gaacacaatt aaaaaaggta aagtaaaagt taaaaaggtt
    47461 gaccaagatg gaaaaccatt agctaatgct gaatttacct tgacggatga ttcatcccaa
    47521 acacaagcag aaaaaacaga tgaaaacgga cttgcaagct tcgatattgt agcaaacaag
    47581 acctacagcc ttgaagagac cggagtacca actggttata caggcgtatt caagcaagaa
    47641 ggaattaccg ttaaagatga tggacaaact tttgaatata cagctacaaa cactttgaaa
    47701 aaaggtttag taaaagttca taaagtcgat caagatggaa aacctttggc aaatgcagag
    47761 ttcactttaa ccgatgaaaa aggtgcaaca caagtagaaa aaacagatga aaatggactt
    47821 gcaagcttca atattgtagc aaacaagacg tatagcctag aagaaactgg agtaccagta
    47881 ggttacacag gtgcatttaa gcaagagggt atcaccgtta aagacgatgg gcaaactttt
    47941 gaatatacag ctacaaataa tttgaaaaaa ggtgtagtaa aagttcataa agtcgatcaa
    48001 gatggaaaac ctttagcaga tgcagaattc accttaacgg atgaaaaagg tgtaaaagaa
    48061 gttaaaaaga cggatgcaac tggtctggct acgttcaaca ttattgcgaa caaaacttac
    48121 aacttagaag aaacaaaagt accaacaggt tacacaggcg cattcaaaca agaaggaatt
    48181 accgtcaaag atgatggaca agtctttgaa tatactgcta aaaacacttt gaaaaaaggt
    48241 gtgatcaaag ttcataaagt ggatgatcaa gggaaagcat tagcaaacgc agaattcact
    48301 ttgacagacg acaaaggtgt aaaagttgta caaaaaacgg acagcaatgg cttggcaaca
    48361 tttaatattg ttgccaacaa aacgtacaaa atcgaagaaa cacaaaaccc taatggttat
    48421 acagggaaat ggtctaaaga aggcatcacc gttgctgacg atgaacaaac ctttgaatat
    48481 acagtaatta atcaccttgt acctgaaaca gtaagaacag gcggaatcgg tacatcagcc
    48541 ttccttcttg tatttctaat tgcaggattg agtatggtgc tactgtatta tcgagaacag
    48601 cacaaagcaa aataaaaaag tgttaatgga aaagaagtaa atatgaaaaa agaaggctga
    48661 cgtatgaaat caaaaaaaat tttttcaaat ttaatgctat ttggtacttt gctaggagtg
    48721 ggattgggcg caagctcaat ctcttctagc cttggactag gggggcaagt agcatatgca
    48781 caacaaaact ataattatca aggaactgga acgattcata tccataaagt aacaaatact
    48841 aatggggatg ggaaccctgt ctcagatatt gataacaaag ggaaagaaat agctttacca
    48901 aatggtgtaa aacctcttga aggcgttgag ttctcttatg tgaaacttac taaagatgaa
    48961 gctaaatcct tgggatggac ggatagtgat actacaaaag gtcaacatat tgatgatgct
    49021 aaagcacgat tattactaaa caaaaaatac agtgaagtaa gtagaatgaa gactgattca
    49081 caagggaata tagacatacc tatacaagta aatacagacg atttaacaga taacttgtat
    49141 ttgattgttg aaacaggaac agtcaaaggt gtagttgaag aagcgtatcc tatgattgtt
    49201 aacgtacctg aactaaacgg atcagatgat caatacttta attatgatgt acatgtctat
    49261 cctaaaaact atttccaatt aaactcagca gagcttgata aaaaagacga agatggaaag
    49321 gctcttgccg gaactgtttt tgaattgcga aaagatgatg gatctgatca ggttgttgga
    49381 acttataaaa cagacagcac aggtaaatta actgtagatg gtttggaatt aggtgactat
    49441 tatttcctag aaaccgactt gggaacagat gctaataaaa caacctattt actagatggt
    49501 aaaaaattca aatttagcgt taaacgtggg gagactgcta aagtagttct tgatgttacc
    49561 aactacaaac gacctgtaat tactaaggta attgataaag aaaccgttga tactggcgaa
    49621 gatgttcaat atacattgaa accatcaatt cctggtaata ttggtaatta cgaaaaatat
    49681 gttgtcaaag atgttctagg tgcagagtta acttatgatt catcagaagt gatcgaagga
    49741 atgactttag atactgatta cagtatcgaa cgtgacggac aaacgacaaa ctatgtcttt
    49801 actgaatcag gtatggataa gcttcagaaa caatttgatg caggggatcg cttctttaca
    49861 attttgatga acgctcatac taatagtaaa gctaaaatgg atacaccact taagaatgat
    49921 gtttcaacga tctacaacaa tggatatgaa cccgaagacg aaacaccagt tgatcacgct
    49981 tatacaatca ttggtgatca ccaattcatt aaaacagatg gagatacaag caaagcattg
    50041 agtggagcaa aatttgtagt gaagaacaaa attgatccga ataaagttac ttacctaaaa
    50101 caagatccaa caacgaaagc tgttacttgg attgcagata aatctaaagc tactgaattc
    50161 gtttccggat cagacggtaa gttccaaatt gaaggcttgc aatataccta tgatgtagat
    50221 tttgataaac aaggtaatcc aacaatcaca gataaaaaaa tcaatgatta tgctcttgaa
    50281 gaagttgttg ctccttcagg atatacacca actagaaatg atgttccatt tgtggtatcg
    50341 aatgatgatg cttctagcga agtaacagac atcaagaacc ttaaaacacc tgaaacagtt
    50401 cgtacaggtg gaattggaac agtagtattt ctagtggttg caggtgtggc aggaacaggg
    50461 ctatatgttg tctacaaacg agaacaacac aaagcttaat gaaataaaat tccctagagt
    50521 gtttttggct ctagggtttc tttttaaaaa gtgtgtgaaa agtaggtatg agcatgaaaa
    50581 agaagaatat caagttgatc atcttaaaaa ttgggatgtt gctatgttta ggagtaggtc
    50641 tatatcctgt aattactcag gcttatacct actaccaaac tcaacaaaca ctaaactcct
    50701 ataaggaaaa atatgatgaa gcttctgaaa aggagaatga agaagtcttc aagagatatt
    50761 cagaagttaa taggaaacga aaagacacag aacaaacctc agtcacgatt cctgaagata
    50821 ataacaatat tccgtcaaat gtaaatcttc agaaggataa attaggtgaa ttagtgggtg
    50881 aaattcttat accaaagatt aatgtggatt tgccaattta tgatggtgta aacgattttc
    50941 aactatcagc aggagcagga atattaaccg gaactgatta tccaactggt gggattggaa
    51001 accattctgt tatatcagct cattcaggca ttcctaataa aaaatatttt acagatttag
    51061 agttgctcaa acttggtgat aaatttttta ttgatatttc agggaaaaaa cttgcttatc
    51121 aagtagatcg gataaaaaca attctaccaa ccgaatttga ggatttgctt cctgtggcag
    51181 gaaaagatta tgtaacactt atgacatgta cacctgttgg agataattct cataggttac
    51241 tcgttagagg ggttagcata ccttaccaaa aaagtattgt tgagaaagaa aagaagaaag
    51301 aagatttaaa ggtaaaggcg cagatgaact ggtatcctat cgtagttgta ggggttttat
    51361 taataatagc tgtgatagtc attattaaaa aaatagtagc aaagagacga ggaatgaaaa
    51421 atgaagaata aaatactact ttttttgact cttatttgca ctttggtttt aacaatttat
    51481 cctgcaaatc agctctatca tcaattcaaa acaaaagaga atgtggcgga aatccaagag
    51541 aaaattgaga aaaaacaaac tccttcaaaa gaggaagtgg caaaaaaagt cgaacaaata
    51601 aaatcgaaca gcggtaacca aggattagac aatactcaga aaacagataa aggagtagac
    51661 aatactcagg aaacagacgg tggatcattt gatattcgtg aagctgaagg aaaggtcaat
    51721 ccatacacag gagatgtttt ttcaaaaaat caaacgaaag attttgaacc atctacctat
    51781 tcaggagcgg ttggttatgt ctatattcca aaaataaatg tgctagagct tttgtttcta
    51841 ggcgcttcac aggagcattt atatagaggt gtggcacaaa tgagtgctac tagtctacct
    51901 ttatcaggta aaggtcatca aacagtaata gcaggtcatc gtggcggtta tacgtcacca
    51961 caatttcttt atgttgatca attagtagcc ggagaccaca tttatataga ttatcttggt
    52021 gaaaaagcag tttatacggt tttccgaaca gacgttatta gaaattacgg tcagaattca
    52081 acactgactg atattcctga tttagatatg ttaacacttt atacttgtca tccatatcct
    52141 cagaactacg atcgtttatt agttagaagt ctaagagatg aaaaagcaaa atacatcacg
    52201 ccacaagtaa cagttggcaa taccgcatac gctgatgcca ttgatagtga atccgtttca
    52261 gataagccag aaactgttac atcttctaat gatttatttg aggatgttga tcgtgccaac
    52321 aaggaattag atggaaataa tgatcttaat attccggaac aaggtagcgt agagatgcaa
    52381 gacagaacgt ttgctgaaaa ggcaagttca atatttacag gaattaccaa taaacatcag
    52441 aaaatgcttt tagtcatttg gactgtatta ggagcattag caagtctaaa ggttcttatt
    52501 cttttaatca aagagtttag aaaagcataa tgaagattat aaaaattgat agagtacagt
    52561 aaaaagggat tgatactatt attgatctct tttttatttt gaatagagct agattcactt
    52621 ttaaataaaa taaaaactga tcttaattcc aaagaaaaag tcaagaaaaa aatacaacaa
    52681 atttttttat cagaatattt atcagaatat tattagaaaa gataaactca aaagcttaat
    52741 ttaatagggt tattggttgt atttagaaat aatgtatgga aaagctataa agcaatagat
    52801 aagatgtaga aaatcttatt tgtgaatgaa aaacaagtca tcgtaaaata ttaataggag
    52861 gtgtagtaat ggtggaaata tcaaaaacag ctttagactc attagctaaa ctatttagta
    52921 gcaggtttga tggtgcagaa tttgttgaat atctaagcca aaacgaaagt ggaaaaactc
    52981 tgatcgaaac actgaaaaaa gactatttga atgaaaatga ctttccgaca tggacgaata
    53041 ttgaagatgc tacatgggat gtagtgtgta cgatatattc tccttgggta acgattgctg
    53101 attttgtttt agataaaatt ggaggggata cataccctga tgccaaatct cttgctacaa
    53161 gtgactttaa aacgttctat ggttatctaa tgggtctaat aggcaatgga atagatttga
    53221 aagttacgcc aaaacaaaca cctgaagaaa ttgaaaaaga aaaaggagag gttcaaatgg
    53281 cttatgcaga tgacagagaa aaagtaaaat tattggaagg taaattatct gaagaagaac
    53341 ttggaaaaat ggtgcaagaa atcaatgaag acagcaacta ttttcctgaa ttgaaggtat
    53401 atcaaaacga cgaagatttc tttaatgaaa tgtttagtaa tagtccatgg gaagcagtga
    53461 gtaaaacaaa tttaggagaa tggaactttc aagatgatct agttgcattt agcaatgatg
    53521 ggtatttacg atcatattca gaaagtgagt atagagctga attaaaaacg tatgaaacgg
    53581 atattctgat gagctattta aatctattag agagtggtca gggagagtta agagataaag
    53641 ttgaagaact attaaaagaa gagctatagg gattatttca acataaacac aagcagaaaa
    53701 ctaccaaaca aaacgtacca tgcaaaaagc cggttaaacc ggctttttgc tctattatga
    53761 gaggagatca aaccatgagt gcaacaaccg atatggcttt agaagcagta ataaaagata
    53821 atacaggaaa tgtcgaaaat gtagaagact ttagcgatat tgcaatcaat ctttcagcat
    53881 ggatttcagc tgtaggagaa gaaaatatcg aatttgagat caagaatatg gatgacttaa
    53941 aagcaaaatt tccttttatc gcatcgaaac taagtcaatt tgccgaggag aaatcgtgga
    54001 atgaattagt tagtttttcc gaaaaaattt atgatattac gcaagaagat gagtgtgttt
    54061 catgggaaat gaataatcaa tacaagaaaa ttgatgcttt cgtcaaatca cgtatagaag
    54121 gaacagcttt tgagtttgaa ggtatagata ccgcagataa ttggttaaat aaagaatcct
    54181 ttattaaaag tatagcggat gatcttgaaa aacaatggtc taaggaagac tatgcagagt
    54241 acctaaaaac agaaagagat acagaggtga aagaaaaact cactgaggta tgtttggatg
    54301 aacatattga tcccaatatc gtaaaacaag aagactatga agtggttatt gataaagttc
    54361 ctactgattt tgaggaagtt gtaaagtata tagttgaaga agcattatcg caatactctt
    54421 cagatgagga agtggacgaa gaaactgttc gtaccagagt ttggcaactg gtttttgatg
    54481 acaaagatta taaatatttg agtgatcaaa actcggttaa ttggagtgta cgattattaa
    54541 ttgatcaaaa tgatattgaa taaaatagag ctgaaaagct ctattttttt taataaaaaa
    54601 actagctatt tttttaaaaa taatttggtt caaaatttat ttttttaact tgataatttt
    54661 ttgccatcct tttttaaatt ggctatatat caaggtttat agtggggata aataaaaata
    54721 catttgacaa aatttacaat atagtttatt atatatttat cttattatga taacacaata
    54781 aaaggaatga tggtatgaaa aagaagaagt ttagtcgatc gttgttagga attttagttt
    54841 tagttgcggt tggtcttagt ggcaagggtt actttcagaa taacagcact caggaacatg
    54901 attttcagat caaaagtagt gttcaggtag gtaataaagc agacagtgaa tcaaaagaat
    54961 ttttagggat acgttatgat ccaaacaaat atccaaataa tatcgtaaac attaatgatg
    55021 ggaaatatac actgacaaaa gaagaccatg aagcattatc agaaaaaggc agtcaaacct
    55081 tttgggtaaa ttatgcagat ttagatcaac ttggaagagc aggagatgtt gaagcgttga
    55141 tcacctatga tgctataaat tctcactcat ctaaaaatgt ggaacgtcca tctttcaaaa
    55201 gttctgtcca tattgcagga gaatacaagg atggagtatt tgatcaagag agagatactt
    55261 gggtcggcaa agattcaaac aatgaaatca ctcagctaga aggatataaa ggttatctct
    55321 ataacaaaag tcattcatta gcatggtcgc taggaggaga tatggaggta cacaatttaa
    55381 cacttggaac gagagcgcaa aatgttggga aaaatgatca gaatggaggg atggcttact
    55441 cagaaacgat tgtacgtgat gcaatctata aaaataagaa tgaaaaagta ttgtataaag
    55501 ttgatccaat ttataaaggg aatgaattgg ttcctcgtgg ttctcatgtc gaagctttct
    55561 cagttaacga taatggaaaa acggtaaacc ttaatgttta tgtatttaac aatcaaaaag
    55621 gaatcaacat caactatcaa aatggtactt gggtaagaga ttagaaattg agaaggtgaa
    55681 gaattggaaa caataagcat caatggatta gataaatcag ttgcaaatcg aataaaaaaa
    55741 ggaaatggct actatcttct gaagaagggt aaatattcag ttgctagaga gttagaaaca
    55801 atgagtatcg tgtatgtagc taagaatcat tttgacgtag aacaggctat ttttgatcca
    55861 gattctgaaa tagggaaatg tgttgaatta tcagagaaaa ctgttagtac aaaaggggcg
    55921 atgatcattg agagagaaat gttagacgaa atcagagtaa tcattcctaa agatacgcag
    55981 atgtgtgagg acagcataag tgacgatgaa ccaaaatcag aaagaaaata aggagatatt
    56041 cagatgagcg aaaaatccat tgaattaaag gaatataagc gaagcgatgg agaaaaatat
    56101 tatcctgtag ccagtttttc taaatcagta tatctggttg gacgtgcatt ggtagttggt
    56161 gcaattggca tatatatttt gcattgggta acaaaggttg tacaactgct tgtagatacc
    56221 ttagtaagta ttcttgtctc gttaaatgat gttaacggaa cagatgattt aggattaaaa
    56281 gaaatgttta aagagttact caatgcgttt tttcccaatt atttaagtta ttcatataat
    56341 gtacaaagct tttttgcaaa gctacacttg gtagacatct ctattttagt attagaagca
    56401 ggaacggtat tctttgttgg atatttggtt actttgatgt tcaggcatca ctatggagaa
    56461 caaagaccat tttttgatga tatggaaagt agaaggttga aaagagaagc tctagaagct
    56521 atggatgtga agtatgatcc aaacaagatt ataggagatg aaacgaacaa caaaaaaaga
    56581 aaacgaaaca aaacgcaatt agaatccgat gtgctatcaa cgcttaaaaa tatcaaaatt
    56641 tctgttcata caagacgtga tctaagtgtc aatgaagata ttcgagaata tatcgtaaaa
    56701 atcccgcaac caaaagggaa gaaagttaga gaaacagttt tacgacaaat tcaagattta
    56761 gggtcgatcc tgacaagagc aacaagcgga cgagccaaat ttggtgaaaa agaagatgaa
    56821 gtttcaggcg attatttcat tttccgagca tcagtaaata ttgaaagaga acgtaaggaa
    56881 agagtaagta ccaaaaacaa agaaacggaa agagcaacat cttcaatcgc agtttcaaat
    56941 tcacaatttt cttttccaat caatctcttt gccgataatt ccaaaaatat ccaaaaccaa
    57001 accgaaaaag ctagaaaata tgccgaaact caaaagcaat ctttagaact ttatctatct
    57061 tctgctgacg tacctgcaag tctaacagat atgcacgttg ggaatacatc tattgaatat
    57121 ttgtttactt tatctcgtac cagaaaaatt caactaagct caatgaaaga agaaattgaa
    57181 acttacttgc atatatcagg tgtaatcgtg cggatcaacg cagggcgtat tcaaattact
    57241 ctaccattag gagaaaatga tcgtattcca attgatgtac catcaatgat caatagcgtg
    57301 ttttaaaaga catcacaaat agaaacagaa aatacaaact accagttcac aaacagaaaa
    57361 gtaccgagaa aagtgaggaa tagaaatggc tatgaaatta ttagagaaag tcaaaaagag
    57421 agccgaaaaa gctgaatcta ttttgctaga taaagaacct aatataaagc ttaatggaaa
    57481 attcagtgtc aatatgttga acacaaaggc taaagacccg acagatgcta tttttggaat
    57541 taagcctgat ggcacaattg ctcatttttg catcagcgaa atgccacaca tgttggttgc
    57601 aggaacgaca ggatcaggga aatcagtatt actgaatgaa attcttgtta cagcaatgtg
    57661 tcactcaacg cctgatgaat taaaaatagg aattgtagac ccaaaaaggg ttgaatttgg
    57721 aagatacaaa aaattgccgt tcatgttggc tgatccaatt acagatatgg atgaagctta
    57781 tgattttttt gaatatcttg tgatcttgat gcatgaacgt tacaaactga tggaaaaagc
    57841 agggttacaa aacatcacgc tatataacga atatgccgaa aaaaatggat tagatagatt
    57901 tccctatgtg atcttattag ttgatgaata ttcacagttg gttggaacac ataaagaagt
    57961 agaaggtttg attgttcaat taggacaaat ggcaagggca gcaggtattc atgttatctt
    58021 agctacacaa tctcctcgat caacagttgt cactggtatt atcaaagcca attttccgtc
    58081 acgtgtttgc cttatggtag caagtgattt agaaagtagg atcgtattag atgaaggcgg
    58141 tggagaaagc ctaagtccaa agggagatat gattattaag ctcgttaatg gaagtatggt
    58201 tagagcgcag ggagcttaca tcagtaacaa agaaattgaa acaattttca atcaccttag
    58261 aaatactatg ccagaaccag aatatgttga ttaccgagca atcgtagctg aatctcgtgg
    58321 tgagggtgga gaagacgagg attttggtgg aattggtaaa gctcctgtaa gaaaatctgc
    58381 taaaagaaat ttgaacacac ctttagcaaa ttcatcatct aaagagccat caattgaaaa
    58441 gaagagtacg tttgctctaa aacttgaaaa aaacaaagaa gaaaaagaaa aacaaggagc
    58501 taaaacacta gaaaaacttc aagccgaaaa attagaaaaa gttttaaaag cattagataa
    58561 agctgattct gaaccgcaaa cctcaaacaa cacgaataat gaacagccga tttcatctaa
    58621 aactaaaccg aaaactactg atccgaaagt ttcagtaaac gtgaccatca caaccgataa
    58681 gtctgagaag aaacaagtaa acggaacgac ttcacctatc atgcaaaaaa tgttgaaagc
    58741 caaaaaacag gtgcataaat gatcattgtc agttcttttt tgaggtggct tacgccatat
    58801 aaatatacag atgaagtagt tgtgaaacga acgaagaaaa gagataaaaa agcggtacta
    58861 actcctaaac agatgcttga tcatggcatt ttaggggtga aggttttaga agtgaataaa
    58921 ccgtttgaat caaatagtgc aagagaaaag ttgcttggag ctgaagccga tgtcgcatat
    58981 ttttaccatg acaaaagcaa gcatcaaata gctattaaat tcaatccaaa atcggcatgg
    59041 cggtatcaag caagagatga cttgaaacgt aggaaactta gaccaatgct ctatccagaa
    59101 ggaaaagaag catttgatag aacccacatc gttcccattg gttttcatgg tacagaatca
    59161 gataaccgcc tattagtagg ttggaacagc aatcaaaata gagttcagat caataaattt
    59221 gagaagaaag taaggaaatt caacgatcga aaaacgatcc tatggtttgc agatatttgt
    59281 aaacagccgg atggaacagc aaagtgggaa gcggtgatat ttgacgaaaa tggtaaaaaa
    59341 gtaatgtctg aaacatggca tgataaagct aaatttgttt ggcagtagca gtagaggtga
    59401 aaaagattgc tagaactttt tgaagcaaat agaacaggct ttaatcaaaa gttagaagtg
    59461 ggagaaacta tagaatataa agataaaaac tatattatta tcggaatcta taacacgaga
    59521 attaacagaa taggtgatcc acgtataacc agtgacttgg tttgtcaatc tgttaactat
    59581 tcacctgatc tgacaagcag gtataagaaa tacgtgttat tagaatatag acacaagatt
    59641 actgatgaaa ttggtgtaaa caacacacga aacatattta agatcggaac agtgattcct
    59701 tatagtaaca acgaagaaaa aatattttat cagatttacg gaatagaaaa gtttgaatat
    59761 gaacatgtcg atctgctagt gacgtaccaa atgcgtttaa tagaaccttg gtcacaggca
    59821 gaaattgata aagcagtaaa attgaaccga ttatcaaaat ttaacgtatt aggaaatgcc
    59881 ttttaaaaat cgcaatacac aaacagaaaa ataccaaaaa attagcaatg aacgtaggta
    59941 agaacatgac aataaataat aataacccta aaaaatataa tgctcttgtc caatgctata
    60001 tcgaaaacaa aattacaatg caagaagtcg ctgatgaatt aggcaaatca aagagcctag
    60061 tatcacacga ttttaagcac aatgtttttc cgaacccgat tgagctgagg aaaaaatttc
    60121 aaaagagagt ttatcaagaa ataggtaagt ggattgatga agggaatgca attgaccctt
    60181 taattgaaaa atatcctttt ttaaaaagca aaaataaaat tcaaaggtta attcgtcaag
    60241 gcgatattca agctaaagaa cctgttttga caaccaatga tttggctaaa ataaatcttc
    60301 ataaacaaat tattgaagac tttgaaaaaa atctttcaac aactgaaatt ttaaaaaaat
    60361 acaatattcc gagaggaaag ctaacatcga ctctttttag agaatatgga gaatcggcag
    60421 ttactaaagc aaaggaagta gcgaatgaga gaaaaaaaca aaaatagtag tcgataccta
    60481 gtagcgactg tagtttttgt ttgtgtattg attttcctgt ttgatttttt gcagattgga
    60541 aaaagcataa ttggaaatac attgagtgca aaggaaatgg ttgctttagg tggtttgtat
    60601 gaaggacact tgttcccgaa tatattcatt tctatcgttt ctcatattaa cttgtaccat
    60661 ttaatttcta atttattgtt cttagtagtt cttgaagaag cactgaatga tcagtatgag
    60721 tggttaacat tattgatcgt tttaatcata tcaggatttc taggaagctt agttcctttt
    60781 ctgctacaca gcaacatcgt aagtatgggg cttagtggag gtatttttgg atggattgct
    60841 ttatttgcga ttgcatacat ccgttttgat agcaagaaac atttattact aagtgttttt
    60901 gtattattga taatcggcat tttactggta tctacttttg aatcaggcga gacaaacgtc
    60961 ttgtcacatg taatcggatt tgtctgtggg atgatcttgg gatttattct aaagaacgtt
    61021 tcattgatca aacaaaagaa ttaaaaggga gtgctaaaaa tgccaaaaaa aaaagaaaag
    61081 aaaattccag tgtttttgtc tgtctcagaa atgttatcac ttcaaatact gttaagaaaa
    61141 aagattgaaa caatagacga agaagttaga aataaaaggg ctatgtacga acaacgtgaa
    61201 gcgctaagag aacgaatcca ctattttgat gatatacaca ctaaaatcta tgaagcacgt
    61261 acaaatcacg aagacgaaag caaattagga ttagctcaac aacgaatatt tgaatggctg
    61321 aatacgaata ctgagcaaga accgttaaga gcaattagta atctttttga atttggaacg
    61381 aagcattggt ttaacgctgt agaaaaggat gctgacttac aagaagcgtt cgatacacta
    61441 agcgaggata gcaaatccat gcgagactta ttgatcactt atctaaatga gcatgaattt
    61501 gaacagacga aagttaaaaa gaaaaataaa aaatagaaag ttgtgatgta tcatgctgtt
    61561 taccttatta ggttatttat gtttagttct tgcttttttt ggagcaattg ctatatttgt
    61621 cggtcagttt caagaaaata atccgataat gcgtaacata ggcattggaa ttgttatcat
    61681 atccttgatt gtatttggtg ccacaaagat ttttgggagt aatgatcagc agtttggtat
    61741 ttctggagtt gataatagtt atgctccaga ttcattggga agagtagtga tagatggaac
    61801 gtttgagaat gtaacgagct acaaaaatgt ttcattgaca gtgaaagaag atgacaagaa
    61861 agcatcagat gacgttctcg ctacgatcaa gccgtctcaa tcacagtttg aagtgtttta
    61921 tacagtcaaa gagaataacc ctgtacgcaa gctaacatta gtttttaacg ttgacggaga
    61981 agaatactat aaagatgtaa aaatcaataa agcatcaacg aaaaagattg tcgaatcagc
    62041 gaaaaaagaa gtagctacag aagaaagtac aactgaatca agttctactg tcgaagttga
    62101 actaccagat aaaattgatt ttgatcctga aaaagtcaaa tcgtttgaat ctgtttctgg
    62161 ttctatggca gatcggttta acactgaagt tttcaaagct atcaaaactg ttaaattgga
    62221 tactgaaaag caaaggatta atgttacctt attcaatgca gttgaatcta tggatcagga
    62281 taaaaaagtt acggttgcaa gagaactagg gaaaagagta attggattgt atcgaggtac
    62341 ggcaaaacca gattcagacg gttcagattt gccagttcac gtagtttatg aagacggtaa
    62401 agtttttgga gactctacag caaatgaagc actaacgatt aaattagcta aatagccgga
    62461 aaattataat aaacaagtgg tgtcacacca ccatcgcata ggtgtgacac cttggagatg
    62521 attactgatg gtagtaaata aagaaaaagt aagaaagtat gttactttaa aaatagcttc
    62581 tgttgaatat ttagaaaagc gagctaaaga agaagaaaaa acaagaggta agaaggtaac
    62641 agtttcagag ttgttagaag aattaattga gagtgaccaa acaataagac gtgcatttac
    62701 caataggtct gttaaaagtt agggtgatac tatctttaat ttcaaccaaa tttctaatat
    62761 atgatgaata gtttccttgt acaaaatatc taattaagag ataaaagtta ggaggattca
    62821 ttcaaaatga ttatccaaaa gtttgactgg aaaatgaaat taggaattag tcttgtaata
    62881 ttgggattta ttattttttt ggggataaaa gcacaaaaaa atcaatctac ttttgaaact
    62941 atgtcttctc aaattattga ggaatgttcc aaaatttatt attgtgagaa caaaactgaa
    63001 gaatctaatg gcttttatta tatcgatcaa ccacatgatg ttgttataaa aatggagtca
    63061 catgaagtaa acgaatggat tcaaagaagt actaatgtat caaaaaagtt agaaagatat
    63121 tctcaaacgt ttttaagtaa agtcattttt tcaaattgca aaaatgaaga aacgtctcaa
    63181 agagtagcta gttctttaga aa
//
LOCUS       DAFBQW010000010        59345 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFBQW010000010 DAFBQW010000000
VERSION     DAFBQW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.010"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(159..1469)
                     /locus_tag="LK889_000850"
     CDS             complement(159..1469)
                     /locus_tag="LK889_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK5095103.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="LK889_000851"
     CDS             complement(1481..2899)
                     /locus_tag="LK889_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5095104.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="LK889_000852"
     CDS             complement(3139..4707)
                     /locus_tag="LK889_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK5095105.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="LK889_000853"
     CDS             complement(4711..5121)
                     /locus_tag="LK889_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK5095106.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="LK889_000854"
     CDS             complement(5118..6041)
                     /locus_tag="LK889_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBK5095107.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="LK889_000855"
     CDS             complement(6038..6916)
                     /locus_tag="LK889_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBK5095108.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="LK889_000856"
     CDS             complement(6903..8240)
                     /locus_tag="LK889_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK5095109.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="LK889_000857"
     CDS             complement(8243..9346)
                     /locus_tag="LK889_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5095110.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="LK889_000858"
     CDS             complement(9420..10928)
                     /locus_tag="LK889_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5095111.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="LK889_000859"
     CDS             complement(10894..11586)
                     /locus_tag="LK889_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK5095112.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="LK889_000860"
     CDS             complement(11608..12582)
                     /locus_tag="LK889_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5095113.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="LK889_000861"
     CDS             12882..13754
                     /locus_tag="LK889_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5095114.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="LK889_000862"
     CDS             complement(14212..16410)
                     /locus_tag="LK889_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095115.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="LK889_000863"
     CDS             16642..17634
                     /locus_tag="LK889_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5095116.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="LK889_000864"
     CDS             17694..19115
                     /locus_tag="LK889_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK5095117.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="LK889_000865"
     CDS             19108..20607
                     /locus_tag="LK889_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK5095118.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="LK889_000866"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="LK889_000866"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK5095119.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="LK889_000867"
     CDS             22199..22882
                     /locus_tag="LK889_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBK5095120.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="LK889_000868"
     CDS             22884..23720
                     /locus_tag="LK889_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK5095121.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="LK889_000869"
     CDS             23731..25485
                     /locus_tag="LK889_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095122.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="LK889_000870"
     CDS             complement(25761..26102)
                     /locus_tag="LK889_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5095123.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="LK889_000871"
     CDS             complement(26170..28341)
                     /locus_tag="LK889_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK5095124.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28510..29409
                     /locus_tag="LK889_000872"
     CDS             28510..29409
                     /locus_tag="LK889_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5095125.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="LK889_000873"
     CDS             complement(29480..30337)
                     /locus_tag="LK889_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5095126.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="LK889_000874"
     CDS             complement(30322..33024)
                     /locus_tag="LK889_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK5095127.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="LK889_000875"
     CDS             complement(33037..34326)
                     /locus_tag="LK889_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBK5095128.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="LK889_000876"
     CDS             34476..35522
                     /locus_tag="LK889_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5095129.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="LK889_000877"
     CDS             35524..37677
                     /locus_tag="LK889_000877"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBK5095130.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38150..39601)
                     /locus_tag="LK889_000878"
     CDS             complement(38150..39601)
                     /locus_tag="LK889_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5095131.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39598..41322)
                     /locus_tag="LK889_000879"
     CDS             complement(39598..41322)
                     /locus_tag="LK889_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5095132.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41315..41929)
                     /locus_tag="LK889_000880"
     CDS             complement(41315..41929)
                     /locus_tag="LK889_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK5095133.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42274..43731)
                     /locus_tag="LK889_000881"
     CDS             complement(42274..43731)
                     /locus_tag="LK889_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5095134.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43772..44692)
                     /locus_tag="LK889_000882"
     CDS             complement(43772..44692)
                     /locus_tag="LK889_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5095135.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44704..45651)
                     /locus_tag="LK889_000883"
     CDS             complement(44704..45651)
                     /locus_tag="LK889_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5095136.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46130..46849)
                     /locus_tag="LK889_000884"
     CDS             complement(46130..46849)
                     /locus_tag="LK889_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK5095137.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(46898..48544)
                     /locus_tag="LK889_000885"
     CDS             complement(46898..48544)
                     /locus_tag="LK889_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5095138.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48723..49313
                     /locus_tag="LK889_000886"
     CDS             48723..49313
                     /locus_tag="LK889_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5095139.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49405..50910
                     /locus_tag="LK889_000887"
     CDS             49405..50910
                     /locus_tag="LK889_000887"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBK5095140.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(50994..52346)
                     /gene="celB"
                     /locus_tag="LK889_000888"
     CDS             complement(50994..52346)
                     /gene="celB"
                     /locus_tag="LK889_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK5095141.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52346..52864)
                     /locus_tag="LK889_000889"
     CDS             complement(52346..52864)
                     /locus_tag="LK889_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095142.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(52880..53206)
                     /locus_tag="LK889_000890"
     CDS             complement(52880..53206)
                     /locus_tag="LK889_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBK5095143.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53219..55198)
                     /locus_tag="LK889_000891"
     CDS             complement(53219..55198)
                     /locus_tag="LK889_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5095144.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55219..55533)
                     /locus_tag="LK889_000892"
     CDS             complement(55219..55533)
                     /locus_tag="LK889_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBK5095145.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55701..55994
                     /locus_tag="LK889_000893"
     CDS             55701..55994
                     /locus_tag="LK889_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK5095146.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56072..57184)
                     /locus_tag="LK889_000894"
     CDS             complement(56072..57184)
                     /locus_tag="LK889_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5095147.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57337..57561
                     /locus_tag="LK889_000895"
     CDS             57337..57561
                     /locus_tag="LK889_000895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095148.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            57571..57750
                     /locus_tag="LK889_000896"
     CDS             57571..57750
                     /locus_tag="LK889_000896"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095149.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            57944..58114
                     /locus_tag="LK889_000897"
     CDS             57944..58114
                     /locus_tag="LK889_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5095150.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58538..59278)
                     /locus_tag="LK889_000898"
     CDS             complement(58538..59278)
                     /locus_tag="LK889_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5095151.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17753 a        12141 c        10382 g        19069 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggctc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    37921 gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
    37981 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt
    38041 ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag
    38101 atttctagac aattttttat aagttatttc attacattgg ctttttcttt tatgattctc
    38161 cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga
    38221 aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt
    38281 gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt
    38341 ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg
    38401 acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt
    38461 ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct
    38521 gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct
    38581 catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc
    38641 gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt
    38701 tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg
    38761 tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa
    38821 attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag
    38881 aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa
    38941 aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc
    39001 cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag
    39061 tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc
    39121 ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca
    39181 gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga
    39241 ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcacgg
    39301 cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa
    39361 attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg
    39421 gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt
    39481 ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct
    39541 tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca
    39601 tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat
    39661 ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga
    39721 accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc
    39781 agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac
    39841 tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc
    39901 aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat
    39961 aaaatgatag gctactcgat taggataacg catttgatac aaatagacat atttttcgca
    40021 aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa
    40081 agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat
    40141 tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg
    40201 catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat
    40261 gctatccaac atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt
    40321 tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat
    40381 agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc
    40441 taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact
    40501 ttttttatct agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa
    40561 attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat
    40621 gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag
    40681 tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc
    40741 taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc
    40801 tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt
    40861 tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac
    40921 gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa
    40981 agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat
    41041 gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt
    41101 tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc
    41161 atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat
    41221 aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt
    41281 tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat
    41341 ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga
    41401 caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa
    41461 taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca
    41521 gattaggaac agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa
    41581 ataggcagag caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga
    41641 tttgaacaga taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa
    41701 atagcagatt gcctcttaaa aaatttcttt tatatatatt ccagcctctt tttattgtga
    41761 tttctttata ctcgaatccg tacaaataaa atagttcatt gactgttttc catgccgcac
    41821 ctaatccaaa gatgatcccg ccggcaaatg tatatatcca gaaatacaag gttagtttta
    41881 taatgaccca gatccggata aataactctt ccaaaacctt ccctaacata tcttctcctc
    41941 caaacattaa gtttatactt tctcattata caaaaaaagg ttgggcaatc agcaccttga
    42001 cctgattatt agagaaacaa acggaaaaat agttcctcat attttgtcgt tttgtgagcg
    42061 aataccgagg gttagctgat tgaacaccgt ttaccaagag tttgtgaaat agccgcttag
    42121 tttcgagtag taagaaaaat tttgtgaata tggctttcca catttttaca tatatttagc
    42181 ttaataccga gaaactggct tttgaacacc gtttattcgg aacaagaggt tgtgacacgt
    42241 cttttgtcac aacccctttg tctttattca ttattattct tgtcggaact catcatattg
    42301 tttttgcatt tctttcaata ccttatcata gccagccttg tccaaagcgt ctttcaattt
    42361 cggtaatgtt tcatctggat caacagtccc tgtattcaat ccatctaaat attgattcat
    42421 gacattagaa atattactta attctgtttt gacactgtca gtgttgaagc tgaatcctaa
    42481 aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt ttggcaatca tgtcatccgt
    42541 aatagactct tgtgtatata gaatcttgtt gtttcctgta ttccaagcag acatatgtgt
    42601 atttggctga taaccatcta gcaatttgat tttatcatct gaaccatcga ctttttccca
    42661 tgcttctccc tcgatccccc aaacaagccc attcaataat tctggatcac tgttaagcag
    42721 ccctagaagt tctactgatt tttctttatt ttttgaagta tttgatacga caaaatttgc
    42781 catttgtgct tgagctgtag attttagtgg aacagtaatt gcttttgaga caagttcttg
    42841 ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg tttctccacg
    42901 cattagccaa gtatttcctt ctagtggata atccttattg ctcgttgcag cgtcacttgg
    42961 aatgagtcct tgtttgtacc agctatgcat tgtacgtaac agtccgatat acgtttcatt
    43021 atcgtattgg ttgatgattt tcgaatcatc tccatccaga tcaatagcaa atggtaacgt
    43081 attccctaaa ggataatcaa agttcccttg gactttgaag ccttgtccaa tggcaaaagc
    43141 tgccgtgttt ggttcttttt catggaattc cttcaagact ttttctgcat cagcgtaaga
    43201 ttggattcca tcaattgaaa gatcgtattt atctagtaaa gctttgttaa aagtaagtac
    43261 ttgttgagca aagacatttc cattgactgg gaaagcatat aattttccat tgaccagatt
    43321 tcctttaata tatgcttcat ctaaatcatt ataagctttt tcagcgtact taggcgctaa
    43381 ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa acatagttgt tcgcaaaggc
    43441 gatatcataa ttttccccgg aagaaatgat gacgttcatt ttcttttcgt aatcgcccca
    43501 gccaatgtaa ttgatattga cttttacacc gatctctttt tcgattcgtt tgtttgctac
    43561 ttccatcaat tgatcgaaat tatctggttt atctccgatt tgatacatct gcaaagtgtc
    43621 cgaattgcta gcttctgcct ggtctttaga accagaacct ccgcatgctg ctaatgttcc
    43681 caacaacaac gctgaaacac ctgtaaaggc tagtttcttc cataacttca tttttcttcc
    43741 tcctaatttt atgatttata tttttgtttc tttattcttt tactccgccg attgttagac
    43801 ctttgacaaa atgcttctgg aaaaatggat agctgatcgc aataggcaac gtcgagatga
    43861 ctacgatcgc cattctggca gattctcctg gaactgaggc taatccgcct gtcaattggc
    43921 ttcctgctcc tgaattttgt gtcagatatt gaatattact ttggattttc atcaaaagat
    43981 actgtagtgg aactagctta tcatcttgga tgtatagtaa agcattgaac cagtcattcc
    44041 agtaacccaa tgcggcaaat aaactgattg tcgcaatacc tggaacagct aacggtaaaa
    44101 cgatctgaac aaatattcgc agttcgctag ctccatcgat ccgtgccgat tcaataatcg
    44161 aatcagagac cgaacgttta aagaatgtgc gcatcacaat aatattgaag ggactgacag
    44221 ccattggcaa gatcaatgcc caaatcgtat cttttaactg aagaagattt gtcataacaa
    44281 gataattggc caccatccct ggtgagaaca acatcgtaat caaacaaaaa acggtaaaaa
    44341 aacgacggaa tggaaagttt gaccgtgaaa tcgcataagc atataaagaa gtagccgtgc
    44401 tattgatcag tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct tggaaaattt
    44461 tatcctgcat ctggtcgaac aagtacgtat aaccaaatat actgaacttt tctggccaaa
    44521 aactgtaccc gtgagtcgac aatatggact catcggtaaa agagatggcg ataacaaata
    44581 taaatggcaa aatacaggaa atagcaaaga gtgcaatcat tatattagag aaaaaattag
    44641 ccgtcttatt gaaagagcgg acttggactc tctccacttt cttttttttc atttttttcc
    44701 tccttagaat aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg tgatcatcaa
    44761 caagacaaag ccgacaactg attggtaaag tcctgcagca gccgtcatac caaaatctcc
    44821 tgtggatgtc aggccattgt agatatacgt atccaaaact tgagtcacat catataatgc
    44881 accggaattc ctaggaatgt tataaaacag gccaaaatca gctcggaaaa tgtttcctac
    44941 ggctaaaatc gttaaaatcg tcattagtgg aataagttgt ggtatcgtca catgtttgat
    45001 ctgctgccat ttgctagctc catcaaccat agcagcttca taataagtcg gatcgattcc
    45061 cataacagat gcaaagtaaa taatgctgtt ataaccaatc cctttccatg taccaatgaa
    45121 caccaagata aacggccaat atttcggttc attataccaa ttgacagctt ctcctccatg
    45181 tccagtgatc cactgattga atattccttt gtcaggactt aaaaaagcgt aaacaaagta
    45241 actgataatg acccaagata gaaaataagg tagtaaggac atcgtttgat aaactttaac
    45301 gagtcgttta tttctcaact cgctcataat gattgcaaat acgactgaga tcagcagatt
    45361 caaaaggata aatgctacat tatataaaat tgtattttta gtgatgagaa atgcttgatc
    45421 cgaagaaaat aaaaatttga aattttccca acccacccaa ggactttccc tcaagctagc
    45481 caagaaccca tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg
    45541 tatgtaaaag aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt
    45601 ataacgccat agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc
    45661 cctttcgatt cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa
    45721 gtatagggaa aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt
    45781 attagagaaa aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg
    45841 agggtcagct gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt
    45901 attagggaga tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta
    45961 acaaaagtct tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac
    46021 ggaaataagc cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt
    46081 tcagatggcg ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc
    46141 tcacttgttc tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag
    46201 gcagccaaaa ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt
    46261 tgaccattcg cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag
    46321 tttctgtctc ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc
    46381 catcaataat cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa
    46441 cctcagctag atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca
    46501 ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa
    46561 aaagatcttc ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat
    46621 cctgagtagc agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat
    46681 acgtaatgat ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat
    46741 cgcttttcac cacttgtcca ttgacttcga tcgttccttt tttgattaat cctttgactt
    46801 ctttgcggct gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc
    46861 atttcaaaag agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc
    46921 gtaaacgcgc tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat
    46981 aaccatacac taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt
    47041 tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca
    47101 taatcaaggt aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc
    47161 gggctacttc atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg
    47221 tcgccaataa agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt
    47281 tcactagaaa cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca
    47341 gcatgttgga aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca
    47401 gtacattact tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta
    47461 acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg
    47521 tcaattttgc cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc
    47581 cagtagacgc gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg
    47641 cactaaaaat cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca
    47701 tgatcttcat aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa
    47761 taataaacgg aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta
    47821 ttttgttttc gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat
    47881 acaaaaggac aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga
    47941 ccgccgtcac atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat
    48001 aaaaaatacg tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat
    48061 tgccttgcaa ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca
    48121 aagaacgcat cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag
    48181 aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac
    48241 ggataaataa tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt
    48301 ttgcaatcgc agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt
    48361 atcccatatt gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg
    48421 gaatgatata gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag
    48481 agcctcgtgc cattttttct tgcggtgtta attgatctaa tgctggtttt ctttgattat
    48541 ccatcggtca gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga
    48601 aactggcaat cgattttata taacttttat aagaacaatt gtttttttga attcggtttc
    48661 gtttttttgg taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat
    48721 caatgacaca agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag
    48781 ttggattgaa ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg
    48841 gacatttagt tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc
    48901 ttatgatgct gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag
    48961 cgggaagtct gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct
    49021 cacatttctt ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt
    49081 acacagtcgg ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt
    49141 ttcggcttcc aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt
    49201 attatgtttt tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat
    49261 tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga
    49321 ttttgtcata gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa
    49381 ttgaataaag cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta
    49441 ttaaaagaaa atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca
    49501 gaaaaagctg aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg
    49561 tttttctgta aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt
    49621 attcaatatt atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg
    49681 gatatccgta aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa
    49741 ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa
    49801 gcaatcttgg atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg
    49861 ttagatggca aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat
    49921 cgtatgatgt cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca
    49981 caagcttaca aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat
    50041 atctcgcctg accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc
    50101 aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt
    50161 gatttactta gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg
    50221 gaagaaagca attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct
    50281 tctcatgaag atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga
    50341 ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt
    50401 caagatgcac aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc
    50461 caatccaatg gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca
    50521 ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc
    50581 ccaggaaaca aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct
    50641 gacatcgcct ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa
    50701 gaaatcaatg aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct
    50761 gaagcgattc gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga
    50821 aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat
    50881 gcaatcgtga agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt
    50941 ctaagaaaaa aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt
    51001 aacttttcac tagctaattc tgctgccttt tctctttctt catttaataa tttattatca
    51061 tagaatttga taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac
    51121 acagcagctt tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc
    51181 cacggcatct gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt
    51241 gtagcagaag ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt
    51301 ggcatcccga aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt
    51361 cctaacactt tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa
    51421 gtcgcacctg atccaccaac ggttacataa gaactgttga attctcctgc caacggataa
    51481 tttgcgcctt gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg
    51541 atgatattcg ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc
    51601 atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt
    51661 ttgaagatat ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca
    51721 atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct
    51781 ggcattttta tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc
    51841 gacataataa tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc
    51901 ctaataccat ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca
    51961 tcagtcatga tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc
    52021 gtgagcggat taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca
    52081 aacacaatgg caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac
    52141 aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat
    52201 ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc
    52261 cccgctgcca tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc
    52321 aggtatttct gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt
    52381 catattgttt aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat
    52441 actgtataaa tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc
    52501 ctccattgtt atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg
    52561 gaatcactaa taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt
    52621 tcgtttggtg ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa
    52681 tcagaatcat cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt
    52741 gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat
    52801 acatgttttt caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct
    52861 gcacaatgat ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa
    52921 cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca
    52981 tttctatttt tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat
    53041 gaagaatctc ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac
    53101 gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat
    53161 caaaagaagt cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt
    53221 attcgatcag tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt
    53281 cctgctgtac ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc
    53341 cttcattttc gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat
    53401 cccactgcat cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa
    53461 cagcgggatg tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt
    53521 gctggatctg ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt
    53581 gcagcaaatc atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt
    53641 tttcagacaa ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg
    53701 gtctatctga ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat
    53761 catcttcatt caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa
    53821 gatcaataga agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat
    53881 agccttttac acttaccact gtaatatttt taccgaattc actgactacg cgattcttta
    53941 atagttgggc actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag
    54001 ctctttcttt ttccagtgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat
    54061 tgatggtata gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga
    54121 ctttgggatt ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc
    54181 tcaatcgaat catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct
    54241 ctactaatga aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg
    54301 atagttcctg atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca
    54361 aataagcaat ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga
    54421 tccgctgcgc cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct
    54481 tgatttcaaa cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct
    54541 gtatgataaa atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga
    54601 ctagctcttc aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg
    54661 aatttccata atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt
    54721 ccccttcgat ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca
    54781 atcgctcctt gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt
    54841 ctgccaaatc atccataaaa agaatggcat cttccaaaag caattggttc aatatatact
    54901 tttgtcgatc ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt
    54961 cttgccgact caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat
    55021 aacctggttt agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca
    55081 agtaattacg gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga
    55141 catactcctt cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta
    55201 tcatccactc ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc
    55261 tgtagggatt gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc
    55321 tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc
    55381 aaaatcacta ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac
    55441 ctcttctctt ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat
    55501 tcctgccgca caaataatca atgctttttt cataaatcaa tcactccttt tatttttata
    55561 aaccaagcat atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga
    55621 aaaaatgtgt atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa
    55681 ttttttcagg aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg
    55741 gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg
    55801 cagaagaagg aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt
    55861 tactgatcga ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga
    55921 tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca
    55981 ctgatttacc gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt
    56041 cactttttcc ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta
    56101 tttttcggat aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga
    56161 tattctcaaa aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg
    56221 ccaaagcacc aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag
    56281 ctagaccacc taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata
    56341 aaacacatcc aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg
    56401 acatcaatcc atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct
    56461 gaaaagggac atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca
    56521 tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt
    56581 ctttgttctt ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa
    56641 ataataagct acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat
    56701 aaacaataaa gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag
    56761 cggttgccat taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc
    56821 atttgatcag ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta
    56881 tgatcgaatg ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga
    56941 ataaaaccac agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa
    57001 aacaaatggc aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc
    57061 acttttcttt tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt
    57121 gtagagactg ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag
    57181 tcatctatga caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca
    57241 actatttttc ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc
    57301 accacaaaat aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt
    57361 ctgagatcaa tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag
    57421 aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt
    57481 ggttctgctg tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa
    57541 accattgtcc actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag
    57601 cgctatacct gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca
    57661 aggcactcga tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg
    57721 tcgttatttt ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg
    57781 aaagagttga gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg
    57841 gttgtgaatt tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg
    57901 gcgagacaga aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa
    57961 ctatttcctt agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc
    58021 cacctacttt cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt
    58081 acttggtttt tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct
    58141 ttccgagaca aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag
    58201 agaaaaaatt acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat
    58261 aaacttgagt cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt
    58321 tgtatatttt ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc
    58381 acaaaattgt ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata
    58441 tttgtttgta taccccttct aaaatcggta gtttccattc aattgatggg tactagttga
    58501 ttttaaaaac tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa
    58561 attttatact gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga
    58621 tggtttctcc agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta
    58681 tatctgagct atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt
    58741 ctttttcgat cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag
    58801 tttctaccag ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc
    58861 ctgtggttag acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc
    58921 cttgcaggac ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
    58981 gtcgattttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
    59041 ttggtgtcgc atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc
    59101 ctggtgctaa gccgtcaatc gccagttttc catctgctcc tgtggttaga cctgactgca
    59161 gaacggttcc attggcatct tgtaactcaa aaacagctcc ttgcaggact tccccgcttt
    59221 ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag tcggtttgct ttcgtcaatt
    59281 ctaccaccgc tgtctgactt ctttcgatct caaactcgat cggtgtcgca tctaattcat
    59341 acccc
//
LOCUS       DAFBQW010000011        56387 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFBQW010000011 DAFBQW010000000
VERSION     DAFBQW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.011"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            330..1175
                     /locus_tag="LK889_000899"
     CDS             330..1175
                     /locus_tag="LK889_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5095152.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="LK889_000900"
     CDS             1456..2676
                     /locus_tag="LK889_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBK5095153.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="LK889_000901"
     CDS             2673..5393
                     /locus_tag="LK889_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5095154.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="LK889_000902"
     CDS             5377..6321
                     /locus_tag="LK889_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5095155.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="LK889_000903"
     CDS             complement(6390..7400)
                     /locus_tag="LK889_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5095156.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="LK889_000904"
     CDS             complement(7580..7924)
                     /locus_tag="LK889_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5095157.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="LK889_000905"
     CDS             complement(7917..8351)
                     /locus_tag="LK889_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBK5095158.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="LK889_000906"
     CDS             8489..9226
                     /locus_tag="LK889_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095159.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="LK889_000907"
     CDS             9219..10430
                     /locus_tag="LK889_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5095160.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="LK889_000908"
     CDS             10634..11416
                     /locus_tag="LK889_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBK5095161.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="LK889_000909"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="LK889_000909"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK5095162.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="LK889_000910"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="LK889_000910"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK5095163.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="LK889_000911"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="LK889_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK5095164.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="LK889_000912"
     CDS             15018..16067
                     /locus_tag="LK889_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBK5095165.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="LK889_000913"
     CDS             16064..16342
                     /locus_tag="LK889_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK5095166.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="LK889_000914"
     CDS             16317..16787
                     /locus_tag="LK889_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBK5095167.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="LK889_000915"
     CDS             16804..17106
                     /locus_tag="LK889_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095168.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="LK889_000916"
     CDS             17123..17494
                     /locus_tag="LK889_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBK5095169.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="LK889_000917"
     CDS             17466..17825
                     /locus_tag="LK889_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095170.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="LK889_000918"
     CDS             17997..19004
                     /locus_tag="LK889_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5095171.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="LK889_000919"
     CDS             19029..20213
                     /locus_tag="LK889_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK5095172.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTMMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="LK889_000920"
     CDS             complement(20650..22233)
                     /locus_tag="LK889_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5095173.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="LK889_000921"
     CDS             complement(22811..22954)
                     /locus_tag="LK889_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBK5095174.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="LK889_000922"
     CDS             complement(23177..23656)
                     /locus_tag="LK889_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5095175.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="LK889_000923"
     CDS             complement(23729..24013)
                     /locus_tag="LK889_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095176.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="LK889_000924"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LK889_000924"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="LK889_000925"
     CDS             complement(24357..25643)
                     /locus_tag="LK889_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBK5095177.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="LK889_000926"
     CDS             25781..26452
                     /locus_tag="LK889_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBK5095178.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="LK889_000927"
     CDS             26418..26906
                     /locus_tag="LK889_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBK5095179.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="LK889_000928"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="LK889_000928"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK5095180.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="LK889_000929"
     CDS             27867..28631
                     /locus_tag="LK889_000929"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK5095181.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="LK889_000930"
     CDS             28864..31077
                     /locus_tag="LK889_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK5095182.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="LK889_000931"
     CDS             31088..31534
                     /locus_tag="LK889_000931"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK5095183.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="LK889_000932"
     CDS             31705..32964
                     /locus_tag="LK889_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBK5095184.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="LK889_000933"
     CDS             complement(33053..34000)
                     /locus_tag="LK889_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5095185.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="LK889_000934"
     CDS             34394..35686
                     /locus_tag="LK889_000934"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK5095186.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="LK889_000935"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="LK889_000935"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK5095187.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="LK889_000936"
     CDS             37579..38454
                     /locus_tag="LK889_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5095188.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="LK889_000937"
     CDS             38720..40603
                     /locus_tag="LK889_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK5095189.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="LK889_000938"
     CDS             complement(40678..40962)
                     /locus_tag="LK889_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK5095190.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="LK889_000939"
     CDS             complement(40952..41686)
                     /locus_tag="LK889_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBK5095191.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="LK889_000940"
     CDS             41815..42438
                     /locus_tag="LK889_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBK5095192.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="LK889_000941"
     CDS             42630..43751
                     /locus_tag="LK889_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBK5095193.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="LK889_000942"
     CDS             43758..46886
                     /locus_tag="LK889_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBK5095194.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="LK889_000943"
     CDS             46905..50099
                     /locus_tag="LK889_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBK5095195.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="LK889_000944"
     CDS             complement(50245..50952)
                     /locus_tag="LK889_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK5095196.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKELFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="LK889_000945"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="LK889_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK5095197.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52447..53100
                     /locus_tag="LK889_000946"
     CDS             52447..53100
                     /locus_tag="LK889_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBK5095198.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53326..54324
                     /locus_tag="LK889_000947"
     CDS             53326..54324
                     /locus_tag="LK889_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBK5095199.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54500..56308
                     /gene="pepF"
                     /locus_tag="LK889_000948"
     CDS             54500..56308
                     /gene="pepF"
                     /locus_tag="LK889_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBK5095200.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18554 a         9728 c        11687 g        16418 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaatgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcac tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aagctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag
    52201 atgtataaaa aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct
    52261 gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg
    52321 aaaggttgaa aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt
    52381 acatttaagc tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt
    52441 agcaaaatgg aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa
    52501 gatttagagg aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa
    52561 aatttctttt atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa
    52621 gctgtcactt tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat
    52681 cttccggatg aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt
    52741 gactttcttc gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc
    52801 ctagaagaac aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa
    52861 cttgcaacag atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc
    52921 tattacctag tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag
    52981 attgctcgaa ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa
    53041 tatggacaat gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa
    53101 atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa
    53161 tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact
    53221 atttttgttt agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt
    53281 cgtgtaaata aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg
    53341 aatgaatata ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt
    53401 cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc
    53461 catcaaaaat cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag
    53521 ttaaaggaat gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc
    53581 tatttgccga ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc
    53641 caatgttcta gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac
    53701 agttaccaag tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg
    53761 atagaaaaaa gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat
    53821 ctcaagaaac aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt
    53881 tccttttgca gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt
    53941 aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa
    54001 tggctgacac gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc
    54061 tacttgcgtc ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt
    54121 ttcttttatt tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa
    54181 aaccagaaag ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa
    54241 tggctttttc ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata
    54301 cacttgtctt ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg
    54361 taaaacaaga tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag
    54421 ttagatgctg tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg
    54481 aagaaaagga gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga
    54541 aaatttaact tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata
    54601 tttggaatta tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg
    54661 cgcttctcaa tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt
    54721 catttatgta tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc
    54781 gctttatgca agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga
    54841 accagaaata ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa
    54901 attggaagtc tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc
    54961 tgctgagcag gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac
    55021 atttgctgtt ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga
    55081 aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt
    55141 gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc
    55201 ttctacttta ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag
    55261 ctctgccaga gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt
    55321 ggtagacgtg gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg
    55381 tttattagaa gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc
    55441 tccaattacc tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat
    55501 gggtgaagaa tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt
    55561 cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata
    55621 tattttattg aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg
    55681 acatagtgtt catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc
    55741 catcttttta gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt
    55801 ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt
    55861 taaaggaaca gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga
    55921 agatgaaaaa ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa
    55981 tgcaaaatat tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg
    56041 gataccgcat ttttattata attactatgt tttccaatac tcgactggct tttctgctgc
    56101 ttcagcactg gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc
    56161 ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga
    56221 tatgacacag gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga
    56281 attagaagaa ttgatcgatc gtttataagg ctttctattt ttgattttct cgaatagaaa
    56341 cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt atttttt
//
LOCUS       DAFBQW010000012        55751 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFBQW010000012 DAFBQW010000000
VERSION     DAFBQW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.012"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            374..3181
                     /locus_tag="LK889_000949"
     CDS             374..3181
                     /locus_tag="LK889_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5095201.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3372..3863
                     /locus_tag="LK889_000950"
     CDS             3372..3863
                     /locus_tag="LK889_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5095202.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3966..4955
                     /gene="manX"
                     /locus_tag="LK889_000951"
     CDS             3966..4955
                     /gene="manX"
                     /locus_tag="LK889_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK5095203.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4988..5791
                     /locus_tag="LK889_000952"
     CDS             4988..5791
                     /locus_tag="LK889_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBK5095204.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5812..6726
                     /locus_tag="LK889_000953"
     CDS             5812..6726
                     /locus_tag="LK889_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK5095205.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6809..7183
                     /locus_tag="LK889_000954"
     CDS             6809..7183
                     /locus_tag="LK889_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBK5095206.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7322..7567
                     /locus_tag="LK889_000955"
     CDS             7322..7567
                     /locus_tag="LK889_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5095207.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7739..8803
                     /locus_tag="LK889_000956"
     CDS             7739..8803
                     /locus_tag="LK889_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK5095208.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9049..9474
                     /locus_tag="LK889_000957"
     CDS             9049..9474
                     /locus_tag="LK889_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5095209.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9467..9916
                     /gene="nusB"
                     /locus_tag="LK889_000958"
     CDS             9467..9916
                     /gene="nusB"
                     /locus_tag="LK889_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK5095210.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10013..10867
                     /locus_tag="LK889_000959"
     CDS             10013..10867
                     /locus_tag="LK889_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBK5095211.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10868..12205
                     /locus_tag="LK889_000960"
     CDS             10868..12205
                     /locus_tag="LK889_000960"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK5095212.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12210..12434
                     /locus_tag="LK889_000961"
     CDS             12210..12434
                     /locus_tag="LK889_000961"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK5095213.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12436..13326
                     /locus_tag="LK889_000962"
     CDS             12436..13326
                     /locus_tag="LK889_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK5095214.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13335..14153
                     /locus_tag="LK889_000963"
     CDS             13335..14153
                     /locus_tag="LK889_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBK5095215.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14260..14709
                     /locus_tag="LK889_000964"
     CDS             14260..14709
                     /locus_tag="LK889_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK5095216.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14727..16409
                     /gene="recN"
                     /locus_tag="LK889_000965"
     CDS             14727..16409
                     /gene="recN"
                     /locus_tag="LK889_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK5095217.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16464..16580)
                     /locus_tag="LK889_000966"
     CDS             complement(16464..16580)
                     /locus_tag="LK889_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBK5095218.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16653..17609)
                     /locus_tag="LK889_000967"
     CDS             complement(16653..17609)
                     /locus_tag="LK889_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5095219.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17780..18148
                     /locus_tag="LK889_000968"
     CDS             17780..18148
                     /locus_tag="LK889_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBK5095220.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18319..18750
                     /gene="mraZ"
                     /locus_tag="LK889_000969"
     CDS             18319..18750
                     /gene="mraZ"
                     /locus_tag="LK889_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK5095221.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18771..19730
                     /gene="rsmH"
                     /locus_tag="LK889_000970"
     CDS             18771..19730
                     /gene="rsmH"
                     /locus_tag="LK889_000970"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK5095222.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19735..20136
                     /gene="ftsL"
                     /locus_tag="LK889_000971"
     CDS             19735..20136
                     /gene="ftsL"
                     /locus_tag="LK889_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK5095223.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20137..22329
                     /locus_tag="LK889_000972"
     CDS             20137..22329
                     /locus_tag="LK889_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK5095224.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22355..23317
                     /locus_tag="LK889_000973"
     CDS             22355..23317
                     /locus_tag="LK889_000973"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK5095225.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23331..24692
                     /locus_tag="LK889_000974"
     CDS             23331..24692
                     /locus_tag="LK889_000974"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK5095226.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24718..25806
                     /gene="murG"
                     /locus_tag="LK889_000975"
     CDS             24718..25806
                     /gene="murG"
                     /locus_tag="LK889_000975"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK5095227.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25821..27041
                     /locus_tag="LK889_000976"
     CDS             25821..27041
                     /locus_tag="LK889_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBK5095228.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27197..28522
                     /gene="ftsA"
                     /locus_tag="LK889_000977"
     CDS             27197..28522
                     /gene="ftsA"
                     /locus_tag="LK889_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK5095229.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28545..29786
                     /gene="ftsZ"
                     /locus_tag="LK889_000978"
     CDS             28545..29786
                     /gene="ftsZ"
                     /locus_tag="LK889_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK5095230.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29803..30480
                     /locus_tag="LK889_000979"
     CDS             29803..30480
                     /locus_tag="LK889_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBK5095231.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30494..31093
                     /locus_tag="LK889_000980"
     CDS             30494..31093
                     /locus_tag="LK889_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBK5095232.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31108..31395
                     /locus_tag="LK889_000981"
     CDS             31108..31395
                     /locus_tag="LK889_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBK5095233.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31444..32226
                     /locus_tag="LK889_000982"
     CDS             31444..32226
                     /locus_tag="LK889_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBK5095234.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32247..32954
                     /locus_tag="LK889_000983"
     CDS             32247..32954
                     /locus_tag="LK889_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBK5095235.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33216..36014
                     /gene="ileS"
                     /locus_tag="LK889_000984"
     CDS             33216..36014
                     /gene="ileS"
                     /locus_tag="LK889_000984"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK5095236.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36063..37589)
                     /locus_tag="LK889_000985"
     CDS             complement(36063..37589)
                     /locus_tag="LK889_000985"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK5095237.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37604..38251)
                     /locus_tag="LK889_000986"
     CDS             complement(37604..38251)
                     /locus_tag="LK889_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBK5095238.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38435..38764)
                     /locus_tag="LK889_000987"
     CDS             complement(38435..38764)
                     /locus_tag="LK889_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBK5095239.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38941..39669
                     /gene="sfsA"
                     /locus_tag="LK889_000988"
     CDS             38941..39669
                     /gene="sfsA"
                     /locus_tag="LK889_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK5095240.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39685..40698
                     /locus_tag="LK889_000989"
     CDS             39685..40698
                     /locus_tag="LK889_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5095241.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40698..41975
                     /locus_tag="LK889_000990"
     CDS             40698..41975
                     /locus_tag="LK889_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5095242.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42038..44740
                     /locus_tag="LK889_000991"
     CDS             42038..44740
                     /locus_tag="LK889_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5095243.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44892..45209
                     /locus_tag="LK889_000992"
     CDS             44892..45209
                     /locus_tag="LK889_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5095244.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45239..45559
                     /locus_tag="LK889_000993"
     CDS             45239..45559
                     /locus_tag="LK889_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5095245.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45667..47127
                     /locus_tag="LK889_000994"
     CDS             45667..47127
                     /locus_tag="LK889_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5095246.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47195..47416
                     /locus_tag="LK889_000995"
     CDS             47195..47416
                     /locus_tag="LK889_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095247.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47447..47629
                     /locus_tag="LK889_000996"
     CDS             47447..47629
                     /locus_tag="LK889_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBK5095248.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47629..48042
                     /locus_tag="LK889_000997"
     CDS             47629..48042
                     /locus_tag="LK889_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBK5095249.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48165..49346
                     /locus_tag="LK889_000998"
     CDS             48165..49346
                     /locus_tag="LK889_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5095250.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49417..49581)
                     /locus_tag="LK889_000999"
     CDS             complement(49417..49581)
                     /locus_tag="LK889_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095251.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49735..49808
                     /locus_tag="LK889_001000"
     tRNA            49735..49808
                     /locus_tag="LK889_001000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49769..49771,aa:Arg,seq:cct)
     gene            complement(49877..51016)
                     /locus_tag="LK889_001001"
     CDS             complement(49877..51016)
                     /locus_tag="LK889_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5095252.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51315..51950)
                     /locus_tag="LK889_001002"
     CDS             complement(51315..51950)
                     /locus_tag="LK889_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095253.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52063..52698)
                     /locus_tag="LK889_001003"
     CDS             complement(52063..52698)
                     /locus_tag="LK889_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBK5095254.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52732..53193)
                     /locus_tag="LK889_001004"
     CDS             complement(52732..53193)
                     /locus_tag="LK889_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBK5095255.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53323..53754)
                     /locus_tag="LK889_001005"
     CDS             complement(53323..53754)
                     /locus_tag="LK889_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK5095256.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53772..54092)
                     /locus_tag="LK889_001006"
     CDS             complement(53772..54092)
                     /locus_tag="LK889_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5095257.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54391..55167
                     /locus_tag="LK889_001007"
     CDS             54391..55167
                     /locus_tag="LK889_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBK5095258.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55182..55385
                     /locus_tag="LK889_001008"
     CDS             55182..55385
                     /locus_tag="LK889_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095259.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55401..55739
                     /locus_tag="LK889_001009"
     CDS             55401..55739
                     /locus_tag="LK889_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBK5095260.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        18490 a         9392 c        11683 g        16186 t
ORIGIN      
        1 aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt
       61 tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata
      121 ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta
      181 taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt
      241 tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat
      301 gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat
      361 tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga
      421 tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag
      481 taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt
      541 acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag
      601 ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat
      661 ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc
      721 tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg
      781 aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga
      841 ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat
      901 gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg
      961 gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc
     1021 tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga
     1081 aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg
     1141 ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt
     1201 tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc
     1261 aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag
     1321 tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg
     1381 atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg
     1441 gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa
     1501 aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga
     1561 ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa
     1621 gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca
     1681 tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga
     1741 atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc
     1801 tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt
     1861 tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc
     1921 ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta
     1981 ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt
     2041 acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac
     2101 agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt
     2161 agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca
     2221 agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt
     2281 taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac
     2341 ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt
     2401 gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca
     2461 ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg
     2521 tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga
     2581 attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa
     2641 agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat
     2701 tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga
     2761 atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg
     2821 tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat
     2881 gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt
     2941 caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc
     3001 attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt
     3061 ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat
     3121 gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg
     3181 aatacacaaa aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct
     3241 ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg
     3301 ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag
     3361 gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag
     3421 tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag
     3481 tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag
     3541 caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag
     3601 caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg
     3661 tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta
     3721 ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag
     3781 aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac
     3841 tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt
     3901 gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta
     3961 taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat
     4021 ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg
     4081 aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag
     4141 acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat
     4201 tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga
     4261 ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca
     4321 ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac
     4381 cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg
     4441 gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag
     4501 ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag
     4561 tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag
     4621 caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata
     4681 ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg
     4741 tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa
     4801 gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag
     4861 gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt
     4921 tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag
     4981 gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat
     5041 ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg
     5101 tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc
     5161 tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc
     5221 atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc
     5281 gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc
     5341 tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga
     5401 aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc
     5461 gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt
     5521 aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat
     5581 caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc
     5641 aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta
     5701 cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga
     5761 ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa
     5821 gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt
     5881 caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca
     5941 gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg
     6001 gaattcttta atacacaccc atacattgct tctccaattt taggggttac attggctctg
     6061 gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta
     6121 ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca
     6181 atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc
     6241 ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc
     6301 tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca
     6361 aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca
     6421 atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg
     6481 gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc
     6541 ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct
     6601 ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct
     6661 ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt
     6721 ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg
     6781 ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt
     6841 ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga
     6901 taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga
     6961 agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc
     7021 cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct
     7081 tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga
     7141 tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc
     7201 taagaggagg ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca
     7261 attatttgtc ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat
     7321 tatgagttgg ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat
     7381 caccagtaaa ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc
     7441 aagtattgga caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat
     7501 tccatctatt ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg
     7561 atcataagtt tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt
     7621 ttggctttat ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg
     7681 tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat
     7741 gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt
     7801 ggttaccagt ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc
     7861 tgttattact ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc
     7921 agcgcaggct caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc
     7981 aaacttagta cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt
     8041 tttagaatat tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat
     8101 gatcgaagaa ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg
     8161 tcatattgcg gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga
     8221 gattgaagtc gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc
     8281 ttttgatacg atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa
     8341 aaaaatcatc gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta
     8401 cgtttccgat atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat
     8461 ttatcaaata gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac
     8521 tggtgtccaa ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc
     8581 atttggacat tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc
     8641 ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta
     8701 cttaccagga atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa
     8761 tcgtgtgttg actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa
     8821 gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata
     8881 cactatagaa gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca
     8941 tagcagtttg cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat
     9001 agagaattcg gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa
     9061 aaaaatttga ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc
     9121 atcgaagtaa ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga
     9181 acttttgcca gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac
     9241 ttagcaaacg aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt
     9301 tcagttccta aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg
     9361 acggatatcg atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta
     9421 gaacaaccaa caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt
     9481 atcaagacac gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc
     9541 agacctaaca aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat
     9601 caacgaagat gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg
     9661 tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat
     9721 caatcggatc tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta
     9781 tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt
     9841 tagtgatgac cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt
     9901 agaagctggg gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc
     9961 gggaaaattc tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga
    10021 attgattaac ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca
    10081 aaaattgaaa gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc
    10141 agccagccaa atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc
    10201 attggtagag cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa
    10261 atacaatcaa gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat
    10321 agatgaagaa aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc
    10381 gatgaattta ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg
    10441 catcatgaaa atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat
    10501 cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac
    10561 tgttactgtt gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat
    10621 tttagtcgta gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc
    10681 tgtagtgata gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa
    10741 atttgatgaa gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc
    10801 aatgactatc acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg
    10861 tgaatagatg gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa
    10921 atttgatgcc gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg
    10981 tttacgtgcc aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc
    11041 tattatgttc aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt
    11101 ccttgtcgtt gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga
    11161 gcacatggag cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa
    11221 gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg
    11281 aatcgcggtc cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa
    11341 aagaagatat ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc
    11401 agcggacaat gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat
    11461 gatcatcggt cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt
    11521 tgctcgggcg atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga
    11581 taccacgatc gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga
    11641 attagctgtt cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt
    11701 gcagcaggcg tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg
    11761 ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca
    11821 gttgaaacag cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac
    11881 gatcgcatta agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg
    11941 ccaagaaact gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca
    12001 acagcagcag cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat
    12061 catgggaaga ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga
    12121 gattcatcaa ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt
    12181 acaagagata gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc
    12241 aagaattaga aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc
    12301 tagattcgtt taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag
    12361 cagaagagac cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa
    12421 acgaggaaag ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa
    12481 aaagaaatct tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg
    12541 agttattcag tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt
    12601 gcttcctttg atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg
    12661 gtgcatacgt attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt
    12721 agaggaaaac ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat
    12781 ggtttgctta caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag
    12841 cttctgctgc ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa
    12901 gctgcagata tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat
    12961 gaaagaaaga cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag
    13021 caacctgaag aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt
    13081 cagataagag atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct
    13141 ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca
    13201 cgtgaggcgt tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag
    13261 gacgtttttt cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag
    13321 aaatgaggga aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat
    13381 ttgaaacaag agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa
    13441 acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag
    13501 gtaaaaaact tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat
    13561 ttgatctatc tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta
    13621 ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc
    13681 agctagcttg gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc
    13741 gttatgcaaa acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt
    13801 cttttatttc actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag
    13861 aagtgattgc tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg
    13921 gaattgttcg tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg
    13981 cacaaggtgg atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa
    14041 atatcgagtt cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta
    14101 ttaatatggc agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa
    14161 ctgtgaaaat caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta
    14221 tcattgatta agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat
    14281 cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa
    14341 atcctgaaaa gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa
    14401 ctgaaactga tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa
    14461 acaaacgaag atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt
    14521 gaccaaatgg aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca
    14581 aatttgattg ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat
    14641 gttttgatga ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat
    14701 tacctttaag ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga
    14761 ttattccgga attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg
    14821 gtgcgggtaa atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa
    14881 gcgattatat acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa
    14941 aacaagaagg attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc
    15001 tgattgttcg acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata
    15061 ttatcacatt agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa
    15121 acgaacatca ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag
    15181 atgtagcttt ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc
    15241 tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata
    15301 tgctccattt ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga
    15361 aattaaaaga agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg
    15421 caggttacgg agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg
    15481 tttcagaact gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata
    15541 tccaaagtgc ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt
    15601 tattggagtt agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac
    15661 agttgaaacg taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca
    15721 ccgaagagct agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac
    15781 tagagcaaaa agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa
    15841 aaatcgcaaa agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa
    15901 acactgagtt tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat
    15961 ttgaccaaat cgaattttac attacgacca atccaggaga gccactgaag cctttggtga
    16021 aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt
    16081 ctgagcaagg tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag
    16141 ttgcacaggc catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca
    16201 tcactcatct gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag
    16261 tgattgatgg acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag
    16321 aaattgcacg tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag
    16381 aactattgaa actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag
    16441 cagctgatag ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat
    16501 ccaatcgtta aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt
    16561 gtttgttttt tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac
    16621 aatctttttt ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa
    16681 taggtagtta cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc
    16741 ctttcagcaa taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata
    16801 gttaaaataa tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg
    16861 attgctgaaa aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc
    16921 attgcctgct ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct
    16981 tggaaaagtt tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct
    17041 ctgaaataaa cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg
    17101 gatacttgaa gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg
    17161 ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata
    17221 tcactttttg gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta
    17281 attgtgataa gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga
    17341 aaataaccgc taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt
    17401 gtcaactttg attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa
    17461 gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta
    17521 ggacgttcta cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc
    17581 tcattatcta atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt
    17641 ataacggata gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca
    17701 taaatttaaa taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat
    17761 aaaaaaggag tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta
    17821 cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg
    17881 ttttaaaata aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt
    17941 gtctagaaat acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat
    18001 gggaatttgt attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta
    18061 tttcaagatg acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat
    18121 cattgtgaac atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa
    18181 aaggcttttt gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg
    18241 atttcccaga aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga
    18301 ggaatggggg ggctttcaat gttgatgggt gaattccaac ataatattga tgcaaaagga
    18361 cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc
    18421 ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct ggaagctaaa
    18481 ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt cttctattcg
    18541 gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag cacgttaaga
    18601 aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg aattgaaatt
    18661 tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt tgacgaaatt
    18721 gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta atgactgaaa
    18781 catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac atcaaaccgg
    18841 atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat ttattaagcc
    18901 aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg
    18961 ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc aaagcgaact
    19021 tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc attttgtatg
    19081 atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt tatcatcaag
    19141 atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat cacgtaatta
    19201 atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag gagaaatttt
    19261 ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc caaacaactg
    19321 gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc
    19381 atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg
    19441 aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga atcagcgtaa
    19501 tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa tatagtacgg
    19561 tacaagatct accaccggga cttcctgtca tacctgacga attccaacca gaactgaaag
    19621 tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac aatcgatcac
    19681 gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag aatgatggca
    19741 gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga tcagccggaa
    19801 caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa
    19861 cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc gattgctatt
    19921 gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc tgaaaaagaa
    19981 atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa
    20041 gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga aaaaggattg
    20101 tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg
    20161 cggtttatgg aaaagaaaaa tctaaatccg ataaacaatc gcaaaaaagt aggtatcatt
    20221 ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc gtatatcgtt
    20281 attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt gtaccaagga
    20341 agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt ggcgctcgca
    20401 gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac ctctggggat
    20461 aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct gcataagaat
    20521 ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa aaaagagctg
    20581 tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc
    20641 gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg
    20701 atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc agacaaagat
    20761 gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat cttgagcggt
    20821 aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc aggtacagtg
    20881 gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga cagcagacta
    20941 caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc agaagaattg
    21001 acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca acgacctact
    21061 ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg taatttcttg
    21121 gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc agctgctatc
    21181 gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca agtagaagat
    21241 gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt
    21301 tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg acgatggtat
    21361 aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga tgatgaagta
    21421 aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta tggacaagcg
    21481 gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa taacggtaca
    21541 atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga agagcgaaca
    21601 actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa
    21661 tatatgagag atgttgtcga aagcgaaaac tacggtagtg cttatggtgt atatagtgta
    21721 cctggataca atgtatcggc aaaaaccgga acagcccaaa tcgcttctga tacaggggga
    21781 tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc ttctgaagat
    21841 ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg aatggcattg
    21901 gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat
    21961 tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa tcttgaagcg
    22021 gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt
    22081 aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg agaaaaactg
    22141 atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc aaaagcagat
    22201 atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga tggttattgt
    22261 gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag ttttacttta
    22321 gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag cattgattcc
    22381 cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg gttattttca
    22441 aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca atagcaaagc
    22501 cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga caggaatttg
    22561 ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt ttgtattagc
    22621 gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga aaagaaacat
    22681 gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca tcttttatct
    22741 tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag atctaccttt
    22801 aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa
    22861 tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt ttgcagctta
    22921 cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct taagtgtttt
    22981 aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt ttatgggaga
    23041 tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc taaaccaaga
    23101 atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg tgatgcttca
    23161 agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa ttcatcatca
    23221 ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt tgatcagcat
    23281 tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa
    23341 tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg
    23401 cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg
    23461 acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata acaggtagtc
    23521 atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca gggattccct
    23581 acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt acggaagttg
    23641 agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg aacggaaaaa
    23701 caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa ggaattgcac
    23761 gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca aaatcagaag
    23821 ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat ttccgccctc
    23881 atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt acaagaaaag
    23941 aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat tatctgatcc
    24001 ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc
    24061 ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac tcaatatact
    24121 ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag cataatgtag
    24181 aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat gaagctatac
    24241 gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta ggagaaatcc
    24301 aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact aaaatggctc
    24361 taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac agaggaaata
    24421 catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta tttggccaaa
    24481 caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata gtaacagccg
    24541 attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt gatgtagttt
    24601 tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact agaggaaaca
    24661 gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg
    24721 aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct tgcctttgtc
    24781 aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc aaaacgaggt
    24841 ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga gattcaagga
    24901 tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct taagagtatc
    24961 cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg tactggagga
    25021 tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac agttatccat
    25081 gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt ggatcggatc
    25141 gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt
    25201 aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa aacatttgat
    25261 ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc acttaaaatc
    25321 aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca agttttgtat
    25381 gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga aatacaaaat
    25441 attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag tgatctgctg
    25501 gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt accggcagtg
    25561 ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat gagtcttgtt
    25621 cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc actaagtcag
    25681 acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa
    25741 gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa
    25801 aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt
    25861 cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat ctggcagaac
    25921 aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca cccctttcag
    25981 attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag tccgagaaaa
    26041 aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat aacggttctt
    26101 ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt cgtcggttag
    26161 ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat gtttctcctt
    26221 atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc caagaaatca
    26281 tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt catcgatcta
    26341 cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat atacatttca
    26401 aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct ttgatcgcaa
    26461 aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa aaagtagcaa
    26521 atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa atcaaagaat
    26581 taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct gaaatcaagt
    26641 acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac ggcaatcaag
    26701 ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag
    26761 atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat ccttacggta
    26821 gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt gaagaaaatg
    26881 cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa acacctggat
    26941 cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt caaatagaag
    27001 ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt
    27061 aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt gaatatatct
    27121 attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga atagtaagta
    27181 ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata ttggaacgac
    27241 atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta ttggagtagg
    27301 aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata aaacggtcca
    27361 agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt
    27421 aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa tgattgcagt
    27481 taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt cagctgccct
    27541 tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag attttactgt
    27601 cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat tagaaatgta
    27661 cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat gcgtggaaaa
    27721 tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg aatcaattct
    27781 ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg gccaaacaac
    27841 aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag aaggtggcga
    27901 atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg cagaagcgtt
    27961 gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag aatttccagt
    28021 agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt cagaaattat
    28081 ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc aaatcgaagc
    28141 attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc ctggtgttgt
    28201 ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg
    28261 gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag caaacttgag
    28321 tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat
    28381 ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg aagaaacaat
    28441 ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa tcaagggttt
    28501 ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa ttctcaattg
    28561 ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa
    28621 atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca
    28681 cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta ggccccaaat
    28741 atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa
    28801 gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc actgcaggaa
    28861 tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg
    28921 gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc
    28981 gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta ttaattatct
    29041 caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa gctttccgtg
    29101 aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc acagcgccag
    29161 gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat
    29221 tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa
    29281 tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt cttaacatca
    29341 caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg
    29401 cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa atgggcgatg
    29461 aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta
    29521 gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta ttagatatgg
    29581 atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg gatatcagac
    29641 gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc gaaaaaaaag
    29701 aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc
    29761 caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc tgacaacttg
    29821 cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa
    29881 gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga attggttaga
    29941 gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa gaaacaagat
    30001 ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg cagaaaggta
    30061 aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag attagcagaa
    30121 gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt
    30181 gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga atctttagct
    30241 gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga agcttctgat
    30301 gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa
    30361 ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga ctttcaaatt
    30421 gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa agacgaataa
    30481 aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt tcggattagc
    30541 tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg ctgtacagcc
    30601 aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg cgaatactca
    30661 tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc agccggctga
    30721 aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga aagaaagaac
    30781 agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga
    30841 accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg gagaagcaac
    30901 cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg attttctaac
    30961 aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga tttttctttg
    31021 tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag atgcgaattt
    31081 gtttgatctg taagaggagg aactacgatt tatactattg cactattact tcatttactt
    31141 caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct ctcttggttt
    31201 ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga accgtatatc
    31261 agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat tttaggggca
    31321 atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg gataactgtc
    31381 catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata ggaggggaaa
    31441 attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt tatcgattcg
    31501 gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac cgaatttctt
    31561 gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga tttgcgtttc
    31621 atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt ccccgattat
    31681 tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta tcctagcaaa
    31741 tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg gatcaaacgc
    31801 tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat tgcaaatgag
    31861 gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt acgcttggaa
    31921 ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga aagaggaaca
    31981 gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc cagacagcgg
    32041 tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa tacaagacct
    32101 gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag agtccagatc
    32161 caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt
    32221 aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc caaaataaga
    32281 cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc ttagatttag
    32341 ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac
    32401 attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac caatcaatca
    32461 tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga
    32521 tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa aaacgtgcac
    32581 atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat gcaacagaaa
    32641 aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga gtattccatc
    32701 aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg gaatgggatg
    32761 aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc aaagaagttt
    32821 tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag
    32881 aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg cctggtgaaa
    32941 atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg atgagagtgg
    33001 aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct tttccgaaat
    33061 tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca ctagatagat
    33121 gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac atgctttttt
    33181 gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat
    33241 taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa
    33301 aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta
    33361 gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta ggacattcat
    33421 tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc tatcgtgcac
    33481 catatgttcc aggatgggat acacatggat tgccgattga acaagtattg gctaataaag
    33541 gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt gaatatgcac
    33601 tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca ggagattggg
    33661 aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc gtatttggta
    33721 aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg tctccgtcaa
    33781 gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct ccatctattt
    33841 ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca gcttttgtca
    33901 tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta aatccaaaat
    33961 ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag
    34021 aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa tttgctggac
    34081 aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca cttgtgattt
    34141 taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct ccaggacatg
    34201 gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca cctgtagact
    34261 caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat gacaaagcaa
    34321 atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta gatttcttta
    34381 ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg gcaactccac
    34441 aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta gaaaaagtag
    34501 agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac cgcggcgatt
    34561 gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat gctgaaaatg
    34621 gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt gctgaacatg
    34681 gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga tttacccacc
    34741 caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc tggtttgatt
    34801 ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc ccagcagata
    34861 tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc acgactagtg
    34921 tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt gctttagatg
    34981 gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa aaagtgatca
    35041 aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat gaagcagatg
    35101 ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa atcagaaata
    35161 cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat actgtttcgt
    35221 acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa gtaatcaaag
    35281 aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt acagttatga
    35341 acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat gtagtttata
    35401 ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat caaacagctg
    35461 tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa atctggtcat
    35521 tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat gaagagtttg
    35581 caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt gataatgtct
    35641 taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg gaagcaaaac
    35701 taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat gcagatattg
    35761 cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat gaacaagtgc
    35821 cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca gacggggaaa
    35881 cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc
    35941 tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta gctgaaggat
    36001 tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac ccccttttgt
    36061 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
    36121 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
    36181 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
    36241 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
    36301 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
    36361 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
    36421 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
    36481 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
    36541 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
    36601 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
    36661 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
    36721 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
    36781 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
    36841 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
    36901 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
    36961 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
    37021 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
    37081 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
    37141 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
    37201 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
    37261 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
    37321 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
    37381 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
    37441 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
    37501 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
    37561 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
    37621 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
    37681 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
    37741 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
    37801 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
    37861 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
    37921 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
    37981 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
    38041 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
    38101 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
    38161 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
    38221 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
    38281 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
    38341 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
    38401 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
    38461 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
    38521 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
    38581 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
    38641 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
    38701 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
    38761 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
    38821 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
    38881 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
    38941 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
    39001 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
    39061 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
    39121 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
    39181 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
    39241 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
    39301 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
    39361 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
    39421 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
    39481 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
    39541 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
    39601 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
    39661 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
    39721 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
    39781 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
    39841 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
    39901 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
    39961 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
    40021 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
    40081 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
    40141 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
    40201 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
    40261 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
    40321 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
    40381 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
    40441 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
    40501 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
    40561 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
    40621 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
    40681 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
    40741 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
    40801 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
    40861 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
    40921 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
    40981 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
    41041 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
    41101 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
    41161 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
    41221 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
    41281 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
    41341 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
    41401 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
    41461 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
    41521 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
    41581 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
    41641 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
    41701 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
    41761 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
    41821 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
    41881 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
    41941 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
    42001 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
    42061 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
    42121 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
    42181 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
    42241 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
    42301 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
    42361 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
    42421 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
    42481 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
    42541 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
    42601 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
    42661 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
    42721 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
    42781 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
    42841 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
    42901 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
    42961 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
    43021 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
    43081 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
    43141 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
    43201 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
    43261 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
    43321 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
    43381 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
    43441 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
    43501 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
    43561 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
    43621 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
    43681 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
    43741 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
    43801 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
    43861 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
    43921 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
    43981 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
    44041 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
    44101 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
    44161 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
    44221 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
    44281 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
    44341 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
    44401 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
    44461 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
    44521 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
    44581 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
    44641 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
    44701 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
    44761 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
    44821 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
    44881 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
    44941 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
    45001 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
    45061 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
    45121 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
    45181 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
    45241 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
    45301 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
    45361 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
    45421 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
    45481 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
    45541 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
    45601 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
    45661 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
    45721 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
    45781 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
    45841 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
    45901 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
    45961 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
    46021 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    46081 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    46141 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    46201 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    46261 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    46321 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    46381 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    46441 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    46501 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    46561 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    46621 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    46681 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    46741 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    46801 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    46861 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    46921 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    46981 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    47041 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    47101 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    47161 gttcctcata atgaattttc taggaggaac tgaaatgatt acaattgcca gtgcaatgca
    47221 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    47281 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    47341 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    47401 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    47461 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    47521 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    47581 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    47641 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    47701 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    47761 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    47821 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    47881 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    47941 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    48001 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    48061 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    48121 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    48181 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    48241 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    48301 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    48361 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    48421 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    48481 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    48541 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    48601 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    48661 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    48721 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    48781 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    48841 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    48901 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    48961 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    49021 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    49081 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    49141 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    49201 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    49261 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    49321 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    49381 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    49441 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    49501 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    49561 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    49621 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    49681 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    49741 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    49801 caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt tgtatttgtg
    49861 ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca gtattgattt
    49921 tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac attgtagttt
    49981 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
    50041 aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca atccctaatt
    50101 cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat ccatttttta
    50161 agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat aattcttttt
    50221 gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa attttcctat
    50281 ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat gttttattaa
    50341 tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga agttcagcaa
    50401 atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac tgcgcagaaa
    50461 ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa tatttatctt
    50521 cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga tcaaacggaa
    50581 ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta aacttaataa
    50641 cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat cccactctat
    50701 ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac gtatcttcgt
    50761 attttctata agtgatagga gatactgttc cttctttata aagttgcatc caatcctcaa
    50821 agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc aagttatatt
    50881 ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat tttcttatct
    50941 tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta tttccacgtt
    51001 tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg aactataaag
    51061 aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc ggcgcaagga
    51121 tgtgctattt gtcgttttaa tggtataatt ggatgtgtat tagatcatcc aatcagaggt
    51181 gtttattatg attaaagtga tttttaaaag aataactgaa cttccagagt ttagattatt
    51241 tgttgattat tggtatgtct ggttttttat tgttgcattg ctgataggtc tagatgtcat
    51301 attagaaaga aaacttaagt aagagttgag ttaataaatt gaattcctaa atgagatatt
    51361 ttgtagccca tattttgttt gccgacatca cctaatacta tggagctaat cgttttttct
    51421 aacagaccaa aatcagataa tctatttaag ctaccatcta ttagctcttg tgaatagcca
    51481 gacatgctga tatttccagt gaaattttcg tttccacgac tcattaagca tgctaacaat
    51541 tgaatctcaa tcgatgttaa ttgattcagt acaagaagat aatactcctc gttatcccat
    51601 gaagcgctgt taattcgaag taaacaattc ttataaagat ttttatacat atctatttta
    51661 ttttgtgcct ttgatttttc tacctcgtca tttatccctt cgaatattcc aatgagttgg
    51721 tccttgttct ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt ttctaatgat
    51781 tcgtttaaat ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt tttagaccca
    51841 aaataaagtg actgcacaac gccaccaata taaggaattg ctccaatacc gccttcaact
    51901 attaattcaa gatattcttt tttggttagt ttttcattgc tattatccat agttacctcc
    51961 tattttgtca tttcctttac aagcattgtg actcctgatg gcactctttt gtataggttt
    52021 tattattgtg cacaatctaa ctcttagtgg ggcgtggttc atttattttt tacttacttt
    52081 actattaatc atatcccatt gagcatagga tttttctatg ttcgatcctc ttgaactaat
    52141 ttctcctgta aaagttactt tatcgccctt tgtaaaggta tcagcattgt ttaagtcctt
    52201 cgcaaaaatt acatatgaaa ctttaggtgt tgcagaaata tcattccaac ttttatcatc
    52261 gtactctttg tctgctataa tggctattct agtagtcatt gattcgataa cagttccttc
    52321 aaaagtatat gttttaccac taatatattt agtaaaagct gtattttgct cagtagctgg
    52381 agtaatagaa taataactat ctaagaaagc accccagtca tgattatttg tctctaaaaa
    52441 ttcttcaaat tgagttaggc ttttattgct gctgttagat gtagtagttt gagtatcaga
    52501 tgatgagctt tcctgtttgt tattgtcatt tgcctcgttt gatgaggaaa gcgagaaacc
    52561 tacggtagta aaaataatcc cgataataag gagaatcata ggtgttttct tatttgtttt
    52621 tttgataaat cttacaatta acatgattaa acctataata aataaaacaa taccagtaaa
    52681 accaaatata ccgtccatta atagatcctc tttctctttt gatattgaat attataaacc
    52741 aagcaattct tttttcttaa aatcgaattc ttcttgatta ataatccctt catctaacaa
    52801 ctctttatac tttttaattt catctgcact tgaaatttgt gtttctttgg aattattatc
    52861 taatatagat tgttgcttct ctatatattt ttttaaattt tccatttcat tccaatattt
    52921 ttttgtaaac ataacggtgt tttcatcttt cgtggccgct aatatacctc cgcgactttc
    52981 gtttccgcct agcaaggtaa attgtatata accattactc atacctggtt tttttaattg
    53041 tacggctgat atatttttaa aggggatagt tttttctcct ttaatacctt gattaactaa
    53101 atttataaat ccttttctaa taattgaaat agaatcatca gttactttaa tcaaggtttt
    53161 tcctggagat tttattaaaa tctctttttc cattttttct tcctcatttc tgtgatatga
    53221 ttttttttgt agaagctcat atatgaggaa agagcccgtg ttgcagcacg ggctttttta
    53281 atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc
    53341 gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt
    53401 attctgaata gaataatact ccataaattt ttcgagatta aactgagatt catcagtcag
    53461 ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc
    53521 aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa aatgagaggc
    53581 gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcac cgtatagatt
    53641 ttcattcaaa aaaatgattc tgggttttgg atagtagaaa ccaggctctt tcatctccat
    53701 ataaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaaac
    53761 catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt
    53821 gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc
    53881 acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga gacatcgaaa
    53941 tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat tatttttcca ctttgctaga
    54001 taaccgttag aatatccaaa attaatttct aactgccgta tagatatctt tttcctcttc
    54061 gccaagtctt ttattatttc ataggtattc atcgataaaa caacctttct aaatgtttac
    54121 aaaaaaagtt tagaaaaata cgcagaaata atttgactaa ttctgagtaa tgagctatac
    54181 tatatcttgt aaacagattt aacaactaaa aagacaacaa aaaacactat tgatatgtaa
    54241 atgcagaccg ccaagaaagc tttaaaatca atgtttttat gtcttattta attatgctct
    54301 tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg ttaaatttgt
    54361 ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa
    54421 caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt
    54481 gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa
    54541 gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac
    54601 gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa
    54661 cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca
    54721 gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta
    54781 aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa
    54841 atctcctatt tagatagcat attatcttct acagattcag taacaattag tcagattgca
    54901 gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag
    54961 aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa
    55021 tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa
    55081 tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct
    55141 caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa
    55201 tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt
    55261 acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg
    55321 gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc gattatcact atgtacgaat
    55381 tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa aatcccaatt ccagaaggct
    55441 atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat
    55501 ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta
    55561 gagttttaaa gaacccaaga tatagaaata ttatcgatat cgagaaaaac aaaaatggat
    55621 ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg
    55681 aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat
    55741 agagcgctag t
//
LOCUS       DAFBQW010000013        53771 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFBQW010000013 DAFBQW010000000
VERSION     DAFBQW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.013"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            9..731
                     /locus_tag="LK889_001010"
     CDS             9..731
                     /locus_tag="LK889_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5095261.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="LK889_001011"
     CDS             789..2828
                     /locus_tag="LK889_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBK5095262.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="LK889_001012"
     CDS             complement(2937..4088)
                     /locus_tag="LK889_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK5095263.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="LK889_001013"
     CDS             complement(4242..4772)
                     /locus_tag="LK889_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK5095264.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="LK889_001014"
     CDS             complement(4871..5221)
                     /locus_tag="LK889_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBK5095265.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="LK889_001015"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="LK889_001015"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBK5095266.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="LK889_001016"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="LK889_001016"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBK5095267.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="LK889_001017"
     CDS             complement(7624..8286)
                     /locus_tag="LK889_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5095268.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="LK889_001018"
     CDS             8470..10116
                     /locus_tag="LK889_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK5095269.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..11189
                     /gene="uxuA"
                     /locus_tag="LK889_001019"
     CDS             10107..11189
                     /gene="uxuA"
                     /locus_tag="LK889_001019"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK5095270.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11297..12850)
                     /locus_tag="LK889_001020"
     CDS             complement(11297..12850)
                     /locus_tag="LK889_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5095271.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12998..14286
                     /locus_tag="LK889_001021"
                     /pseudo
     CDS             12998..14286
                     /locus_tag="LK889_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14412..15491)
                     /locus_tag="LK889_001022"
     CDS             complement(14412..15491)
                     /locus_tag="LK889_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5095272.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15594..16034)
                     /locus_tag="LK889_001023"
     CDS             complement(15594..16034)
                     /locus_tag="LK889_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5095273.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16091..16501)
                     /locus_tag="LK889_001024"
     CDS             complement(16091..16501)
                     /locus_tag="LK889_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK5095274.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16681..17115)
                     /locus_tag="LK889_001025"
     CDS             complement(16681..17115)
                     /locus_tag="LK889_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5095275.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17215..18063)
                     /locus_tag="LK889_001026"
     CDS             complement(17215..18063)
                     /locus_tag="LK889_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5095276.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18452..20218
                     /locus_tag="LK889_001027"
     CDS             18452..20218
                     /locus_tag="LK889_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBK5095277.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20320..21579
                     /locus_tag="LK889_001028"
     CDS             20320..21579
                     /locus_tag="LK889_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5095278.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21726..23027
                     /locus_tag="LK889_001029"
     CDS             21726..23027
                     /locus_tag="LK889_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5095279.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23030..23881
                     /locus_tag="LK889_001030"
     CDS             23030..23881
                     /locus_tag="LK889_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5095280.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23894..24709
                     /locus_tag="LK889_001031"
     CDS             23894..24709
                     /locus_tag="LK889_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBK5095281.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24860..26026
                     /locus_tag="LK889_001032"
     CDS             24860..26026
                     /locus_tag="LK889_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5095282.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26256..26903
                     /locus_tag="LK889_001033"
     CDS             26256..26903
                     /locus_tag="LK889_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK5095283.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26987..27931
                     /locus_tag="LK889_001034"
     CDS             26987..27931
                     /locus_tag="LK889_001034"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBK5095284.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28066..29517
                     /locus_tag="LK889_001035"
     CDS             28066..29517
                     /locus_tag="LK889_001035"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBK5095285.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29538..30374
                     /locus_tag="LK889_001036"
     CDS             29538..30374
                     /locus_tag="LK889_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK5095286.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30374..31033
                     /locus_tag="LK889_001037"
     CDS             30374..31033
                     /locus_tag="LK889_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5095287.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31159..31848
                     /locus_tag="LK889_001038"
     CDS             31159..31848
                     /locus_tag="LK889_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095288.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31854..32810
                     /locus_tag="LK889_001039"
     CDS             31854..32810
                     /locus_tag="LK889_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBK5095289.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32854..33366
                     /locus_tag="LK889_001040"
     CDS             32854..33366
                     /locus_tag="LK889_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5095290.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33622..34170)
                     /locus_tag="LK889_001041"
     CDS             complement(33622..34170)
                     /locus_tag="LK889_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5095291.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34259..35440)
                     /locus_tag="LK889_001042"
     CDS             complement(34259..35440)
                     /locus_tag="LK889_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK5095292.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35676..37184)
                     /locus_tag="LK889_001043"
     CDS             complement(35676..37184)
                     /locus_tag="LK889_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBK5095293.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37343..37534
                     /locus_tag="LK889_001044"
     CDS             37343..37534
                     /locus_tag="LK889_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095294.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37737..38654
                     /locus_tag="LK889_001045"
     CDS             37737..38654
                     /locus_tag="LK889_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBK5095295.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38647..39720
                     /locus_tag="LK889_001046"
     CDS             38647..39720
                     /locus_tag="LK889_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095296.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39868..40386
                     /locus_tag="LK889_001047"
     CDS             39868..40386
                     /locus_tag="LK889_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095297.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40367..40759
                     /locus_tag="LK889_001048"
     CDS             40367..40759
                     /locus_tag="LK889_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBK5095298.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40835..41770
                     /locus_tag="LK889_001049"
     CDS             40835..41770
                     /locus_tag="LK889_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBK5095299.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41903..42682
                     /locus_tag="LK889_001050"
     CDS             41903..42682
                     /locus_tag="LK889_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK5095300.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42675..42992
                     /locus_tag="LK889_001051"
     CDS             42675..42992
                     /locus_tag="LK889_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK5095301.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43236..44846
                     /locus_tag="LK889_001052"
     CDS             43236..44846
                     /locus_tag="LK889_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5095302.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45003..45851
                     /locus_tag="LK889_001053"
     CDS             45003..45851
                     /locus_tag="LK889_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5095303.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46082..46561
                     /locus_tag="LK889_001054"
     CDS             46082..46561
                     /locus_tag="LK889_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095304.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46594..48822
                     /locus_tag="LK889_001055"
     CDS             46594..48822
                     /locus_tag="LK889_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5095305.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            48951..50594
                     /locus_tag="LK889_001056"
     CDS             48951..50594
                     /locus_tag="LK889_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK5095306.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50667..51212)
                     /locus_tag="LK889_001057"
     CDS             complement(50667..51212)
                     /locus_tag="LK889_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBK5095307.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51235..51720)
                     /locus_tag="LK889_001058"
     CDS             complement(51235..51720)
                     /locus_tag="LK889_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5095308.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51710..52522)
                     /locus_tag="LK889_001059"
     CDS             complement(51710..52522)
                     /locus_tag="LK889_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBK5095309.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52661..>53771)
                     /locus_tag="LK889_001060"
     CDS             complement(52661..>53771)
                     /locus_tag="LK889_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK5095310.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17905 a         9783 c        10381 g        15702 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    10261 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    10321 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    10381 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    10441 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    10501 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    10561 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    10621 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    10681 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    10741 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    10801 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    10861 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    10921 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    10981 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    11041 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    11101 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    11161 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    11221 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    11281 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    11341 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    11401 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    11461 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    11521 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    11581 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    11641 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    11701 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
    11761 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    11821 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    11881 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
    11941 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    12001 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    12061 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
    12121 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    12181 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    12241 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    12301 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    12361 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
    12421 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    12481 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    12541 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    12601 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    12661 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    12721 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    12781 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
    12841 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    12901 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    12961 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    13021 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    13081 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    13141 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    13201 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
    13261 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
    13321 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    13381 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
    13441 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
    13501 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    13561 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    13621 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
    13681 agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
    13741 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagcccc
    13801 agaagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc aaacagtacc
    13861 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc
    13921 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
    13981 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
    14041 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
    14101 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg tcctgaaacg
    14161 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
    14221 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
    14281 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
    14341 atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
    14401 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
    14461 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
    14521 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
    14581 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
    14641 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
    14701 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
    14761 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtattt
    14821 aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
    14881 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
    14941 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
    15001 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
    15061 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
    15121 tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt
    15181 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
    15241 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
    15301 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
    15361 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
    15421 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
    15481 acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa
    15541 atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc
    15601 tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc
    15661 aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt
    15721 ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc atcacccgtt
    15781 tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta
    15841 ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat
    15901 aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag gaggggagtg
    15961 tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg attaactaaa
    16021 tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac
    16081 acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt
    16141 ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa
    16201 gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat
    16261 aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca
    16321 attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg
    16381 ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat
    16441 tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca
    16501 taaaattacc acctttaatt atattttata caattgaatt ttacacaata taattgtgga
    16561 aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt
    16621 tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc atcataaaca
    16681 ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac gatggacctg
    16741 atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc
    16801 catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt
    16861 gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg
    16921 agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac catacaaggt
    16981 aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc
    17041 aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa aatcgatttc
    17101 cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt
    17161 attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc
    17221 gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa
    17281 ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac
    17341 aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt
    17401 aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact
    17461 catgaccata acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga
    17521 agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa taaacagatt
    17581 aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata
    17641 ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa
    17701 aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga
    17761 agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc ttccgaaaag
    17821 tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt
    17881 caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta atattcctag
    17941 accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc
    18001 taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt
    18061 catttattca cttccttaat tcattaataa tactcttatc ttattataac ttacttttca
    18121 ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta tgtttcattc
    18181 ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag aaaattggag
    18241 aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag actaaaactt
    18301 ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa
    18361 actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac ttatagtgat
    18421 gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc
    18481 ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag
    18541 gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca taacaactga
    18601 aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta
    18661 ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg
    18721 ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt
    18781 gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac
    18841 tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa
    18901 cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg
    18961 agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt acttgacgga
    19021 ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata
    19081 cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa
    19141 cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca
    19201 tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta
    19261 taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc
    19321 agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact
    19381 aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa
    19441 agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg
    19501 gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat
    19561 ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc
    19621 atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt
    19681 cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa
    19741 cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt
    19801 gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg
    19861 gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga
    19921 gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa
    19981 acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga
    20041 agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt actttaatca
    20101 gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca
    20161 gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa
    20221 actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa
    20281 gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa
    20341 cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat
    20401 ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat
    20461 tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg
    20521 aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg
    20581 cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga
    20641 cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac
    20701 aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt
    20761 gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag
    20821 aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt
    20881 ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat
    20941 gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta
    21001 gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac
    21061 ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc
    21121 attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc
    21181 aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg
    21241 gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg
    21301 aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa
    21361 gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat
    21421 gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa
    21481 aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca
    21541 gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt
    21601 tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat
    21661 cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca
    21721 ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac
    21781 ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg
    21841 ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat
    21901 tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca
    21961 ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat cttttagacg
    22021 cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag
    22081 tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc
    22141 tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt
    22201 atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt
    22261 ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg
    22321 tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg
    22381 ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat
    22441 gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag
    22501 gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg
    22561 caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct
    22621 ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag
    22681 acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca
    22741 cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt
    22801 tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc gtaggcggag
    22861 gagctggttc tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc
    22921 cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt
    22981 cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt
    23041 tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt
    23101 aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc
    23161 aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac
    23221 tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt
    23281 cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta
    23341 tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac
    23401 tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta
    23461 ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa
    23521 aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg
    23581 gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca
    23641 agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg
    23701 aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa
    23761 ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt
    23821 attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata
    23881 ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac
    23941 tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt
    24001 tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat
    24061 tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt
    24121 ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac
    24181 ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt
    24241 tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg
    24301 ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt
    24361 ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta
    24421 tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc
    24481 ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag
    24541 tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt
    24601 tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct
    24661 tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc
    24721 gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct
    24781 gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt
    24841 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
    24901 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
    24961 ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
    25021 aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
    25081 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
    25141 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
    25201 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
    25261 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
    25321 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
    25381 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
    25441 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
    25501 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
    25561 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
    25621 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
    25681 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
    25741 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
    25801 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
    25861 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
    25921 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
    25981 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
    26041 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
    26101 tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata
    26161 aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa
    26221 tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta
    26281 gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg
    26341 ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag
    26401 caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata
    26461 tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa
    26521 ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg
    26581 atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga
    26641 atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg
    26701 atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa
    26761 gcggtacggc aacagattgg gatttggata aagatttagg tactacttat tgggaagtca
    26821 aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac
    26881 tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta
    26941 gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac
    27001 gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat
    27061 gactacccag gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag
    27121 ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg
    27181 gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct
    27241 tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat
    27301 gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat
    27361 cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt
    27421 tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat
    27481 caaattgcct ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca
    27541 gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac
    27601 agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta
    27661 tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat
    27721 ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg
    27781 tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg aaattttttc
    27841 tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga
    27901 atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc
    27961 gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt
    28021 tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca
    28081 gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata
    28141 cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc
    28201 gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc
    28261 ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct
    28321 cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg
    28381 ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca
    28441 gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga
    28501 tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt
    28561 gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct
    28621 tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt
    28681 gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt
    28741 atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa
    28801 aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag
    28861 gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca
    28921 gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc
    28981 gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa
    29041 gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct
    29101 acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat
    29161 caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa
    29221 gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta
    29281 gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca
    29341 cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa
    29401 aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa
    29461 atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag
    29521 aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt
    29581 ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg
    29641 tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt
    29701 ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac
    29761 ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt
    29821 caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa
    29881 aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag
    29941 atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca
    30001 atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg
    30061 ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg
    30121 attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg
    30181 agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc
    30241 tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa
    30301 tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt
    30361 cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc
    30421 tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg
    30481 aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt
    30541 atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct
    30601 gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac
    30661 gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc
    30721 aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat
    30781 cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct
    30841 agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa
    30901 gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg cactgattta
    30961 ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatgca
    31021 tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt
    31081 ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata
    31141 tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac
    31201 caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa
    31261 ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc
    31321 tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga
    31381 cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct
    31441 acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct
    31501 ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct
    31561 ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag
    31621 cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag acgttcagaa
    31681 aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt
    31741 caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg
    31801 aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact
    31861 atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag
    31921 gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta
    31981 aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc
    32041 aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata
    32101 gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac
    32161 ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac
    32221 agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc
    32281 tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt
    32341 atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat
    32401 atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta
    32461 gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac
    32521 attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag
    32581 gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa
    32641 tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa
    32701 agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt
    32761 ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg
    32821 taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa
    32881 gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc
    32941 tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa
    33001 agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc
    33061 tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct
    33121 tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa
    33181 ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc
    33241 gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg attcacttat
    33301 caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc
    33361 aattgactat aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat
    33421 catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt
    33481 ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc
    33541 tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct cggcttatcc
    33601 tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc
    33661 ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa
    33721 ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc
    33781 acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac
    33841 taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca
    33901 atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc
    33961 atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga
    34021 aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt
    34081 aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga
    34141 aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt
    34201 gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatatt
    34261 ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa
    34321 ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac
    34381 cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg
    34441 ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt
    34501 catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga
    34561 ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc
    34621 cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca
    34681 tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga
    34741 aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat
    34801 agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga
    34861 tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag
    34921 acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt
    34981 ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt
    35041 ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc ataaataatg
    35101 gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca attggcatca
    35161 atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa
    35221 caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg
    35281 ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca
    35341 caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta
    35401 atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa
    35461 ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta
    35521 cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg
    35581 tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa
    35641 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
    35701 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
    35761 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
    35821 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
    35881 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
    35941 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
    36001 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
    36061 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
    36121 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
    36181 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
    36241 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
    36301 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
    36361 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
    36421 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
    36481 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
    36541 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
    36601 tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc tgggccagga
    36661 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
    36721 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
    36781 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
    36841 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
    36901 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
    36961 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
    37021 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactagcttc
    37081 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
    37141 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
    37201 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
    37261 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
    37321 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
    37381 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
    37441 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
    37501 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
    37561 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
    37621 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
    37681 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
    37741 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aatgcggatc
    37801 aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc
    37861 tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc
    37921 ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg
    37981 aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat
    38041 ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca
    38101 cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt
    38161 tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca
    38221 aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag
    38281 atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct
    38341 tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat
    38401 ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa
    38461 aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag
    38521 aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg
    38581 aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt
    38641 ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat
    38701 catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa
    38761 gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac tccgacgaat
    38821 acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa
    38881 ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta
    38941 gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca
    39001 tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc
    39061 gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat
    39121 tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa
    39181 aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg
    39241 atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg
    39301 ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata
    39361 ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat
    39421 cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg
    39481 acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg
    39541 gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata
    39601 acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct
    39661 gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga
    39721 ttggataagt tggattacca gatgtcattc agataattct gttccaaagg acttgcggca
    39781 gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt
    39841 gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta
    39901 ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca
    39961 atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc
    40021 atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc
    40081 caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca
    40141 gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga
    40201 ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac
    40261 cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt
    40321 tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat
    40381 ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta
    40441 tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac
    40501 gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa aaggcgatga
    40561 tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc
    40621 gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg
    40681 gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt
    40741 caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt
    40801 cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat
    40861 gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg
    40921 ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg
    40981 cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt
    41041 gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct
    41101 tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc
    41161 ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga
    41221 aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc
    41281 cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga
    41341 cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac
    41401 cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc
    41461 cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag
    41521 cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat
    41581 aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg
    41641 taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc
    41701 tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc
    41761 tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt
    41821 ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt
    41881 tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt
    41941 gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt
    42001 gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc
    42061 cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac
    42121 attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg
    42181 ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga
    42241 aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc
    42301 tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt
    42361 tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt
    42421 cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc
    42481 aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc
    42541 aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc
    42601 taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca
    42661 ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg
    42721 tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct
    42781 ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag
    42841 atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga
    42901 ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac
    42961 ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat
    43021 tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga
    43081 tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt
    43141 tattttagga attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat
    43201 gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa
    43261 cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc
    43321 cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg
    43381 ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga
    43441 ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt
    43501 ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt
    43561 tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag
    43621 ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat
    43681 atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg
    43741 tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca
    43801 tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta
    43861 tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt
    43921 ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac
    43981 taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga
    44041 tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat
    44101 tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa
    44161 cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa
    44221 tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga
    44281 ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa
    44341 gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc
    44401 tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag
    44461 caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga
    44521 tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt
    44581 gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc
    44641 gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat
    44701 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
    44761 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
    44821 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
    44881 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
    44941 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
    45001 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
    45061 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
    45121 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
    45181 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
    45241 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
    45301 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
    45361 ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
    45421 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac gcttatctga
    45481 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
    45541 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
    45601 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
    45661 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
    45721 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
    45781 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
    45841 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca tcatcttgga
    45901 ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta tttctatcaa
    45961 tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca tatagtttag
    46021 taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag gagttaaaat
    46081 aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag actatgataa
    46141 agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg gccttgctca
    46201 gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata ctggagaaaa
    46261 atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag cgatcttagc
    46321 tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt atgatccaga
    46381 aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag aagtagatac
    46441 caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac aaaaagacgg
    46501 agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc taggatatta
    46561 atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact aacagaaaac
    46621 cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag ccatcggaca
    46681 agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga tcctgaaatg
    46741 aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca attagccaaa
    46801 gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc agatatggac
    46861 aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga acaagaccaa
    46921 gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac actgatccag
    46981 aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct agttcaaagg
    47041 atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga aatcgttcga
    47101 gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg agatgcctct
    47161 gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt accgacagga
    47221 atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa ttaccaagga
    47281 aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa aatggatctt
    47341 cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca aaaacaagaa
    47401 cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa agctaacgcc
    47461 atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta taagcaagga
    47521 caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac tgattcagat
    47581 gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga acttcaggac
    47641 atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg aagaaccatt
    47701 agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat tctgcgctta
    47761 gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa acaagaagaa
    47821 acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag cgatatcgaa
    47881 ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc tgcttctgac
    47941 atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg acaaatcatg
    48001 aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt ccaaggaaaa
    48061 acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc acttagccgg
    48121 caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg aaaatacgaa
    48181 ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt tgacaaaaag
    48241 aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga atctgggtca
    48301 atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat tgccgaattt
    48361 tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg ttcgtcaaga
    48421 gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg gttaaatgaa
    48481 aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact tcgtttagca
    48541 gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat tagtgacggt
    48601 cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat ccagtctgtt
    48661 ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg ccaagacaaa
    48721 gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga cttgaatgaa
    48781 tttcccaagc aaatgattca actgattgcc cgctatctat gataaaataa agctcaacga
    48841 acgcacttag cagattcgtt gagcttttcg tttctagtta ccggtaacaa ggatacttct
    48901 aaaatagaat cggttataat caagagggac cgttaaagga gtgaaacgat atgatacaag
    48961 aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta tcctgctagt ttcaaagata
    49021 gcaatggcga tggtcaagga gacatcaatg ggatccgtga aaaacttaat tatttgaaag
    49081 atcttggtat cggctttatc tggatcaatc cgatctatca atctccattt gtcgataatg
    49141 gttatgacgt aagtgactat caagatatag aagagagatt cggaacgatg gaagattttg
    49201 atttattgct aaaagaagca catgaattag ggatcaaagt gattatggat cttgtcatca
    49261 accatagttc ggatcagcat caatggtttg aagaatcgaa aaaatctaaa gaaagcccgt
    49321 atcgcgatta ttacatttgg gtcgacgggg tagatggcaa agaaccgaat aattggacat
    49381 cgattttcgg cggttctgct tgggagtatt ctcatgaaac tggacagtat tatctgcatg
    49441 tttttgcaaa agaacaacca gacttaaatt gggaaagcga gaaattgaaa gaagaactat
    49501 tcaatatgat ccgatggtgg ttggataaag ggatagatgg atttcgttta gatgcgattt
    49561 ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga gaaccctttc tcaccctttc
    49621 aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa acatgttttt gaagagtacg
    49681 atatcatgac tgtcggagaa gcaagtggtg tcactgcaga agaaggacca caatgggtag
    49741 ggaaagacgg ttatttcgat atgatattcg aatttgatca tatccatatc tggcagcaag
    49801 aaaaagaagg gcaattagat gtccttaaat tgaaacttgc attaagcgct tggcaaactt
    49861 cattggatgg tatcggatgg aacgctttat atatggaaaa ccatgatgtt cctcgtgccg
    49921 tttcagtgtt tggagatact cgtcccgatt tttgggctat gtctgcaaaa gcaatcgcta
    49981 tgatgtattt ctttttgcaa ggtacgccat ttatttatca aggacaggaa atcggaatga
    50041 cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt agattcgaaa agactttata
    50101 aaaggctgct tgcggaaggt aagacaagag aagaagcatt ggatatcatt cgtgaaacaa
    50161 cgagagacaa cagcaggaca cccatgcaat ggacttcgga acaatatgct ggtttttcta
    50221 cacatgaacc ttggctgatc actaatccaa ataccaaaac gatcaatgta gaacaacaag
    50281 aatatgaacc agagtcggtc ctgcaattct ataagaacat gatccgcatt agacaaacca
    50341 ataaaggtct gatatacggt tcttataaag aatatcttca tgaacatcca caactttatg
    50401 tctatgaaag atatttagaa gatgaacaat atcttattat ggtaaactta acagaatcac
    50461 ttgctgacta tgaacttcca aaagaagcag atcagtcctg gacattactc ttgtcaaatt
    50521 cctctagcgg agaatttgaa gctaaaggca tactagcacc ttatgaagct cggttatata
    50581 aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt ggactttgcg aggaagtttt
    50641 tcagattgct ctgtcaatta ctcatttcaa taattcgctc taggaccacc gatatattta
    50701 ggacgagagc gaagggctgt cacgtgagct tgtccaaggg ttttcaaacg aggtctgaca
    50761 ggtacttgga catgtttgat atgcataccg attgatgtgt caccgatatc gataccggct
    50821 tgtccaacta cgtgttccac ttcgacagga tcattgaact ggtcaaaagc agcaacagaa
    50881 caagcacctc cagcatgtag tgcaggaaca acagagacaa tttcgaaatt ttttgcttct
    50941 gccactgttc gttctactac taatgctcgg ttcaaatgtt cacaaccttg gacagctagc
    51001 gcaatttctt taggatccaa caactctttt aacgtacgga tgatccattg accaacttcc
    51061 gcactggaat tttttccaat atgtcctcca acgacttcgc ttgtgctgca tcctaaaaca
    51121 aaaagatcac cttttttcaa gtgtgcctca gccaatatgt cattaactat ttcagtcaat
    51181 tggtctttta attctttttc tgacaacttc attttctaaa accttctttt tcaattaata
    51241 ttgcttccgt gcttgaatac gttgaagact tgcagttata ggaaaggcaa gtcccattcc
    51301 aatcagattt tggaagatgt ttcctggtat agaagccact ccagccggcc aatcatataa
    51361 aatccaaccg gcgataccat agccgatgat cataactaag cttcctaata aaagccctaa
    51421 tccccacata cgagtagaag atttgctgaa agaaccagca acatatcctt gtaacccgtg
    51481 gatcactaat gaaaaaactg cccactgcgg atatcctgaa atcaaatcga tcaatagacc
    51541 actggaagca ccaacaacta atccacccgt tggaccataa agaaaagcag cggtatagat
    51601 cccggcctcg cataatgtca caaatccatt cgtagccggt acagggatca aaattgttaa
    51661 tgaaagggca actgtcaacg caatcagcat agcatagatc gttaattgct tagttttcat
    51721 ttctcattcc tcctttttga ataccgccag accagacact tccggtatga tcgttcatca
    51781 gcacgccatt agaaatacaa tgggcaacaa attttttact ttctgctact gctttggtaa
    51841 aatctgcccc taacacaaga ttcgctgtta ttgcagaaga aaaggcacaa ccggccccgt
    51901 ttactgttgt agtgtcgatt tttttatgag aaaatacctc ataatgatca attgaataga
    51961 gaagatccac agcttcatct ccagccatcc ttgtgccgcc tttgatcaca atgttttttg
    52021 ttcctgaagc agtgagttct ttagcgagat ggatcatttc tgttaacgaa gagggaagag
    52081 cttgaccaga aagtaaagca gcttctgcca aattaggcgt aatcatcgta acaagtggaa
    52141 aaagttcttc gatgagctgc gaaatataca attgattgat cacagcttca gtttctttga
    52201 atgccaaaac agggtctaaa atgattggga tattttgttt tgtttttatg aagtctcgaa
    52261 caaccatgat tgcttcactt gaattcaata acccaatttt gataccatct aatgaagtca
    52321 ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa agatatagat tccagcataa
    52381 atcgctcatt aatatctagt gcccctatag aagtcaaggc gcttagtccg aatagaccaa
    52441 aattctcaaa agtttttaaa tctgtttgga ttcctccacc agcaaatgga tcagaaccgc
    52501 caattgtcaa gattcttttc ataacatttt cctccatgaa taagagtata acgaaatttt
    52561 caaaaaagaa aaacagccaa ttggcatatt tttatctcat ccaatttaaa aaaacaaaaa
    52621 gaaaagaaag accaaaatgg atctttcttt ccttaaatag ttaggacttt tgtgatttta
    52681 attctttctc gatttgaagc cgttgcactt tcattgtagc tgttcgcggg attgcatcaa
    52741 aatcacggat gatagggacg ttcaagcgag gaagttcatg gacttgctcc caccatcgat
    52801 tccaatccat ttctttatca ggagctaaag caataatcgg ttgtggagaa tgatttttat
    52861 cccttacaat cactacttct gccaaaaaat ctaacgaatc taataaaaag tcttcaatag
    52921 ctaatgtact attgatcgtt tcgatcaaat ccacttgccg atctttcaaa aataggtggc
    52981 catgcttatc cataaaaccg taatctccgc tatcccacca atcgccataa acattttcct
    53041 ggaaccgtgc atcttccttg taataagtca aagcccgtcc tttagacagt aattgtatat
    53101 gaccatctgt gttttcaggt aggacattcc caactgaatc tgtaatccga gcgcttgtca
    53161 tgtcttccag tccaacgccc atatctcttg catcacttgt tttcaaactc tctagtgtat
    53221 gagccttaag aatcattggt ccgcattcac tttggccata tacttgaaga aataccgcat
    53281 cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg gttgtttata gcatcaaaag
    53341 tggagtgata gtatcggata cccgaaaaag cttctggatg ctccttagca gtaaaacgcc
    53401 actgaacaaa gttattcgga tgggtctcta gtgcaattgg ccggtgagct tccaacatat
    53461 gaacaacttt tgaactttgt gcgtttgcta aaggcatcat aggaaatccc atggacatca
    53521 aagaagagat accaatattg aatctggaat gcactggaga gatgtgaaaa gcgattaatt
    53581 tcttttccga aatttttgtg aaaactgttt tttgccattt cgtccgccat cccatagaat
    53641 gagcagaatg acaaataagt ttagggatac cagttgtacc agaagtatgg gtcatataag
    53701 aaatttcatt tttgtctaaa aatgattgag aaacagacaa agcaggttgt tccattaaat
    53761 aaagaatagg a
//
LOCUS       DAFBQW010000014        52406 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFBQW010000014 DAFBQW010000000
VERSION     DAFBQW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.014"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..705)
                     /locus_tag="LK889_001061"
     CDS             complement(<1..705)
                     /locus_tag="LK889_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5095311.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
     gene            1393..1551
                     /locus_tag="LK889_001062"
     CDS             1393..1551
                     /locus_tag="LK889_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095312.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1623..2849
                     /locus_tag="LK889_001063"
     CDS             1623..2849
                     /locus_tag="LK889_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5095313.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="LK889_001064"
     CDS             complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="LK889_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK5095314.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="LK889_001065"
     CDS             complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="LK889_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK5095315.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4308..4775)
                     /locus_tag="LK889_001066"
     CDS             complement(4308..4775)
                     /locus_tag="LK889_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBK5095316.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4895..6433)
                     /locus_tag="LK889_001067"
     CDS             complement(4895..6433)
                     /locus_tag="LK889_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK5095317.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6513..7199
                     /locus_tag="LK889_001068"
     CDS             6513..7199
                     /locus_tag="LK889_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK5095318.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="LK889_001069"
     CDS             complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="LK889_001069"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5095319.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="LK889_001070"
     CDS             complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="LK889_001070"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK5095320.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8685..9815)
                     /locus_tag="LK889_001071"
     CDS             complement(8685..9815)
                     /locus_tag="LK889_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK5095321.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9815..11998)
                     /locus_tag="LK889_001072"
     CDS             complement(9815..11998)
                     /locus_tag="LK889_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK5095322.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11995..13020)
                     /locus_tag="LK889_001073"
     CDS             complement(11995..13020)
                     /locus_tag="LK889_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5095323.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13065..13892)
                     /locus_tag="LK889_001074"
     CDS             complement(13065..13892)
                     /locus_tag="LK889_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK5095324.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13889..14956)
                     /locus_tag="LK889_001075"
     CDS             complement(13889..14956)
                     /locus_tag="LK889_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK5095325.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14982..15818)
                     /locus_tag="LK889_001076"
     CDS             complement(14982..15818)
                     /locus_tag="LK889_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5095326.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15815..16684)
                     /locus_tag="LK889_001077"
     CDS             complement(15815..16684)
                     /locus_tag="LK889_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5095327.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16754..18037)
                     /locus_tag="LK889_001078"
     CDS             complement(16754..18037)
                     /locus_tag="LK889_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK5095328.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18051..19736)
                     /locus_tag="LK889_001079"
     CDS             complement(18051..19736)
                     /locus_tag="LK889_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBK5095329.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20011..21036
                     /locus_tag="LK889_001080"
     CDS             20011..21036
                     /locus_tag="LK889_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5095330.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21631..25305)
                     /gene="rpoC"
                     /locus_tag="LK889_001081"
     CDS             complement(21631..25305)
                     /gene="rpoC"
                     /locus_tag="LK889_001081"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK5095331.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="LK889_001082"
     CDS             complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="LK889_001082"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK5095332.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29708..30676
                     /locus_tag="LK889_001083"
     CDS             29708..30676
                     /locus_tag="LK889_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5095333.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30694..31599
                     /locus_tag="LK889_001084"
     CDS             30694..31599
                     /locus_tag="LK889_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5095334.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31596..32402
                     /locus_tag="LK889_001085"
     CDS             31596..32402
                     /locus_tag="LK889_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5095335.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32538..33527
                     /locus_tag="LK889_001086"
     CDS             32538..33527
                     /locus_tag="LK889_001086"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBK5095336.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33844..34368)
                     /locus_tag="LK889_001087"
     CDS             complement(33844..34368)
                     /locus_tag="LK889_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBK5095337.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34655..36436
                     /gene="scm"
                     /locus_tag="LK889_001088"
     CDS             34655..36436
                     /gene="scm"
                     /locus_tag="LK889_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK5095338.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTSSTTSDSSDSST
                     SSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSN
                     NWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(36591..37577)
                     /locus_tag="LK889_001089"
     CDS             complement(36591..37577)
                     /locus_tag="LK889_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK5095339.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(37600..38166)
                     /locus_tag="LK889_001090"
     CDS             complement(37600..38166)
                     /locus_tag="LK889_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBK5095340.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(38180..38596)
                     /locus_tag="LK889_001091"
     CDS             complement(38180..38596)
                     /locus_tag="LK889_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK5095341.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(39053..40987)
                     /locus_tag="LK889_001092"
     CDS             complement(39053..40987)
                     /locus_tag="LK889_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5095342.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            41268..42224
                     /locus_tag="LK889_001093"
     CDS             41268..42224
                     /locus_tag="LK889_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBK5095343.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            42314..43417
                     /locus_tag="LK889_001094"
     CDS             42314..43417
                     /locus_tag="LK889_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK5095344.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            43430..44383
                     /gene="menA"
                     /locus_tag="LK889_001095"
     CDS             43430..44383
                     /gene="menA"
                     /locus_tag="LK889_001095"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK5095345.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(45197..46543)
                     /gene="gor"
                     /locus_tag="LK889_001096"
     CDS             complement(45197..46543)
                     /gene="gor"
                     /locus_tag="LK889_001096"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK5095346.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(46677..47930)
                     /locus_tag="LK889_001097"
     CDS             complement(46677..47930)
                     /locus_tag="LK889_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK5095347.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(47936..48859)
                     /locus_tag="LK889_001098"
     CDS             complement(47936..48859)
                     /locus_tag="LK889_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK5095348.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(49080..51572)
                     /locus_tag="LK889_001099"
     CDS             complement(49080..51572)
                     /locus_tag="LK889_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK5095349.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(51579..52046)
                     /locus_tag="LK889_001100"
     CDS             complement(51579..52046)
                     /locus_tag="LK889_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBK5095350.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(52183..52256)
                     /locus_tag="LK889_001101"
     tRNA            complement(52183..52256)
                     /locus_tag="LK889_001101"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52220..52222),aa:Arg,seq:acg)
     gene            complement(52314..52387)
                     /locus_tag="LK889_001102"
     tRNA            complement(52314..52387)
                     /locus_tag="LK889_001102"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52351..52353),aa:Pro,seq:tgg)
BASE COUNT        15209 a        11053 c         9582 g        16562 t
ORIGIN      
        1 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
       61 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      121 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      181 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      241 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      301 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      361 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      421 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      481 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      541 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      601 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      661 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
      721 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
      781 ttttataatt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
      841 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
      901 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttaaagtgga tagcgggttg
      961 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
     1021 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
     1081 tgtttatcgt tattaatagt gaataggaaa aaacctattt ttgatcattg acaattaaat
     1141 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
     1201 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
     1261 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
     1321 atgtgcttac ccaaagcaat tcaaaaattc taatcttttt taggagatga ttttataaaa
     1381 ttgagagtct acttgaataa aaaacaaacc tgtgagtttc taggaattga taataatact
     1441 ttagataagt ggattcgtat atataatttt ccagtaatca gaattgacgg tatagttcgc
     1501 tttgacatag aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt
     1561 ctcgccgcat atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa
     1621 ttttgcctac aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     1681 tcagagaaat cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat
     1741 tggctattaa agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga
     1801 atgcaaattc actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat
     1861 ggcttcctag atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa
     1921 caattagtaa tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg
     1981 caatgaacta tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga
     2041 aagaatacgc aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag
     2101 aaatactaaa ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac
     2161 ctaaaaaaat tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag
     2221 aagagcttat tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc
     2281 ttcatgacta tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt
     2341 atgctttgca atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt
     2401 tggataaata tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac
     2461 ccgaaattgt aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga
     2521 gattaaaaat taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag
     2581 aagtaaattg ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac
     2641 gacatcctga attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag
     2701 ctagacaagg cggagcaaat atgaatcaaa tatctagtgc tttaacacac tcagatattt
     2761 ctacaacgag aatatacgta aatacaccag atgtagtaga taaaactgtt tttgaagcct
     2821 ttcagagagg attgaataaa tgtgattagt atgtgttctt aaatgtgttc ttaaacaaga
     2881 aaaagacact tttcaaacaa ttgaaaagtg ccttgaaaca ttgatatata aataatatta
     2941 acgtttagag aattgagatg ctttacgagc ctttttgaga cctggttttt tacgttcgac
     3001 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     3061 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     3121 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     3181 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     3241 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     3301 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     3361 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     3421 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     3481 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     3541 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     3601 cactttgtct gcattgatga cgatgacgaa atcaccagta tccaaatgag gtgtgaaagt
     3661 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     3721 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     3781 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     3841 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     3901 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     3961 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     4021 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     4081 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     4141 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     4201 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     4261 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     4321 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     4381 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     4441 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     4501 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     4561 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     4621 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     4681 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     4741 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     4801 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     4861 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     4921 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     4981 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     5041 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     5101 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     5161 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     5221 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     5281 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     5341 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     5401 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     5461 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     5521 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     5581 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     5641 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     5701 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     5761 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     5821 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     5881 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     5941 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     6001 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     6061 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     6121 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     6181 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     6241 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     6301 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     6361 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     6421 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     6481 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     6541 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     6601 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     6661 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     6721 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     6781 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     6841 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     6901 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     6961 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     7021 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     7081 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     7141 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     7201 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     7261 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     7321 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     7381 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     7441 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     7501 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     7561 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     7621 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     7681 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     7741 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     7801 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     7861 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     7921 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     7981 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     8041 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     8101 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     8161 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     8221 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     8281 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     8341 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     8401 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     8461 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     8521 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     8581 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     8641 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     8701 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     8761 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     8821 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     8881 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     8941 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     9001 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     9061 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     9121 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     9181 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     9241 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     9301 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     9361 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     9421 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     9481 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     9541 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     9601 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     9661 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     9721 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     9781 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     9841 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     9901 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     9961 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    10021 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    10081 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    10141 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    10201 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    10261 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    10321 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    10381 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    10441 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    10501 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    10561 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    10621 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    10681 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    10741 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    10801 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    10861 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    10921 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    10981 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    11041 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    11101 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    11161 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    11221 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    11281 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    11341 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    11401 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    11461 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    11521 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    11581 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    11641 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    11701 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    11761 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    11821 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    11881 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    11941 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    12001 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    12061 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    12121 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    12181 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    12241 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    12301 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    12361 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    12421 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    12481 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    12541 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    12601 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    12661 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    12721 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    12781 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    12841 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    12901 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    12961 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    13021 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    13081 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    13141 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    13201 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    13261 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    13321 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    13381 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    13441 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    13501 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    13561 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    13621 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    13681 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    13741 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    13801 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    13861 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    13921 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    13981 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    14041 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    14101 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    14161 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    14221 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    14281 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    14341 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    14401 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    14461 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    14521 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    14581 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    14641 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    14701 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    14761 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    14821 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    14881 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    14941 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    15001 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    15061 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    15121 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    15181 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    15241 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    15301 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    15361 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    15421 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    15481 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    15541 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    15601 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    15661 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    15721 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    15781 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    15841 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    15901 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    15961 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    16021 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    16081 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    16141 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    16201 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    16261 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    16321 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    16381 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    16441 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    16501 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    16561 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    16621 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    16681 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    16741 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    16801 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    16861 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    16921 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    16981 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    17041 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    17101 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    17161 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    17221 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    17281 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    17341 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    17401 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    17461 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    17521 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    17581 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    17641 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    17701 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    17761 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    17821 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    17881 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    17941 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    18001 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    18061 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    18121 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    18181 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    18241 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    18301 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    18361 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    18421 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    18481 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    18541 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    18601 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    18661 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    18721 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    18781 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    18841 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    18901 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    18961 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    19021 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    19081 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    19141 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    19201 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    19261 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    19321 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    19381 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    19441 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    19501 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    19561 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    19621 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    19681 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    19741 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    19801 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    19861 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    19921 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    19981 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    20041 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    20101 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    20161 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    20221 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    20281 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    20341 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    20401 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    20461 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    20521 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    20581 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    20641 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    20701 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    20761 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    20821 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    20881 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    20941 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    21001 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    21061 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    21121 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    21181 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    21241 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    21301 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    21361 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    21421 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    21481 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    21541 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    21601 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    21661 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    21721 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    21781 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    21841 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    21901 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    21961 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    22021 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    22081 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    22141 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    22201 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    22261 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    22321 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    22381 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    22441 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    22501 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    22561 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    22621 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    22681 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    22741 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    22801 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    22861 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    22921 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    22981 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    23041 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    23101 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    23161 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    23221 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    23281 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    23341 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    23401 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    23461 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    23521 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    23581 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    23641 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    23701 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    23761 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    23821 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    23881 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    23941 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    24001 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    24061 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    24121 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    24181 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tctttaagaa
    24241 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    24301 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    24361 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    24421 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    24481 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    24541 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    24601 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    24661 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    24721 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    24781 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    24841 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    24901 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    24961 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    25021 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    25081 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    25141 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    25201 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    25261 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    25321 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    25381 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    25441 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    25501 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    25561 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    25621 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    25681 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    25741 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    25801 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    25861 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    25921 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    25981 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    26041 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    26101 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    26161 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    26221 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    26281 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    26341 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    26401 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    26461 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    26521 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    26581 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    26641 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    26701 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    26761 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    26821 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    26881 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    26941 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    27001 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    27061 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    27121 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    27181 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    27241 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    27301 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    27361 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    27421 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    27481 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    27541 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    27601 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    27661 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    27721 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    27781 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    27841 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    27901 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    27961 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    28021 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    28081 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    28141 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    28201 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    28261 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    28321 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    28381 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    28441 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    28501 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    28561 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    28621 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    28681 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    28741 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    28801 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    28861 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    28921 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    28981 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    29041 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    29101 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    29161 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    29221 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    29281 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    29341 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    29401 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    29461 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    29521 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    29581 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    29641 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    29701 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    29761 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    29821 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    29881 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    29941 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    30001 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    30061 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    30121 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    30181 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    30241 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    30301 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    30361 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    30421 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    30481 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    30541 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    30601 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    30661 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    30721 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    30781 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    30841 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    30901 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    30961 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    31021 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    31081 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    31141 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    31201 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    31261 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    31321 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    31381 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    31441 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    31501 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    31561 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    31621 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    31681 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    31741 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    31801 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    31861 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    31921 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    31981 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    32041 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    32101 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    32161 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    32221 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    32281 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    32341 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    32401 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    32461 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    32521 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    32581 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    32641 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    32701 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    32761 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    32821 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    32881 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    32941 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    33001 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    33061 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    33121 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    33181 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    33241 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    33301 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    33361 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    33421 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    33481 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    33541 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    33601 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    33661 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    33721 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    33781 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    33841 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    33901 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    33961 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    34021 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    34081 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    34141 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    34201 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    34261 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    34321 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    34381 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    34441 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    34501 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    34561 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    34621 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    34681 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    34741 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    34801 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    34861 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    34921 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    34981 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    35041 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    35101 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    35161 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    35221 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    35281 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    35341 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    35401 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    35461 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    35521 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    35581 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    35641 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    35701 tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga
    35761 tagcacggat acatcagctt caagcgacag tacagatact acctctacca caagtgatag
    35821 cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgacagcac
    35881 ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg atagcacgga
    35941 cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata gcacggacac
    36001 gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca cggacacgtc
    36061 agcttcaagt gacagtacag atactacctc taccacaagt gatagcacgg acacgtcagc
    36121 ttcaagtgac agtacagata catcctctac cacaagtgat agctcagatt catcaacttc
    36181 aagcgatagt acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt
    36241 ctcaaaaaca agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga
    36301 caatggaaac aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat
    36361 aacactcgca ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa
    36421 gaaatcgaaa aggtaaaaaa atattcaaga taaaaaagag caacgtcgct aacggacgtt
    36481 gctctttatt tttgcttttt atcataaata caagaactac aattcaattt gtcaaaaaac
    36541 acgtagattt ccttctgaga aatttattta cgtatcgaac ctttccctat tcagtctttg
    36601 cgatccaaaa tcagttgggt aagggaaaag agattctctt ttgttcgaag agaggtggca
    36661 ggtaatttct ttatttcctt caaagccttt tctgtgtaag aggccgctaa cttcctagct
    36721 tcttccacgc ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc
    36781 attgccgctt tcttctctaa ataaggtagt agctttggtt tgtttttttg caaagagtag
    36841 atcaacggta aagaatagac cccttgacgc atgtcttcta gtacaggttt gccgatttcc
    36901 tggctctctt ttgtataatc taaaatatca tcgataatct gaaaggccat cccgatagaa
    36961 agtccgatat ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca
    37021 acagaacaac ttaattggaa cagttcagct gtcttacctg aaatattttc caaatattct
    37081 tttacagaaa gttcataatt atagcgatca tccatctgcc ctaattcgcc attcaatact
    37141 ttttccatac tgcgagaatt caattggaga cttttcaagg aagtggagta atcagacaat
    37201 agtttaaaac agcagacaaa taaatagtct cctgcataga cagaaacgac attaccaaac
    37261 tgactacgga tattaggctg accacgtcgc agatccgctt catcaacgat atcatcatgg
    37321 atcagtgtgg ccgtatggag catttcgata gaagctgcca aagcgatggc tttgtctcga
    37381 tcttgctctg gtccaaattg tgagaacaat agctgatagg caggacgcaa cagctttccg
    37441 ccggcatgga tcgtttcttt gatggcttgt tcgacaggtt tgtttttcag gcggacagtt
    37501 tcttccatcc gttttagtgt ttctgtcaat tctttactta aagcaggata gttttcccac
    37561 attggatgta gcttcataaa aaaactcctt gtgtgacgat tattttttgg taaacattgg
    37621 gctccatttt tgtttggttc cctgctcata atctttttgg aatcgttgaa gtgtctgcac
    37681 atgtcgtaaa agataattcg ctgcgatccc gattgcaatc cctgataaaa tccctaaaac
    37741 tgaaaggact ggcaagtaca acatgactgt ccatgattgt gcaatgaaag aagccatcaa
    37801 aagctgtccg acattatgca tgaaaccgcc ggccgcacta attccgatga tactgacacg
    37861 ttttggtcct aattgcttga ccaataacat tccaaaataa ctaagcaaag caccactcat
    37921 actataaagg aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca
    37981 aagtagtaag aagctgtctt gttttttcat ggtaaaaatg gcaacgatcg tgattagatt
    38041 ggctaagcct agttttgcac caggcgcaaa agaaaaagga taagggatca tattttccaa
    38101 taaaccaatg atcactcctt gtgcaacaag tagcgaaata taaatcattt tttgtaattt
    38161 actcatggtg tgtgaaggtc taataaatca gactgtcttc gtcacctcca tctgttgaac
    38221 ggacttcgat cactaattta tgtggcaaac agacgatggt ttcgccattt ttcgttgccc
    38281 agcctcgacg tacgcagatc tggtcatcgc aatctgcttc ttttatccgt actcgatcgc
    38341 catctatctc gatcaagtta taatctccgt gttcatcttt gtaagtatat gtgttgctct
    38401 cttctccagc catcaaaggg aatgttttga tttctgtgcc atccacacgc agtaccgctt
    38461 ctttttctgc tgttgcggtg ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa
    38521 gaacagtcaa aagcaaaata ataaaaacat cccaaggttt gagcagactt ttttcagcaa
    38581 attctttaag gctcattttt gttctccttt ttccagatta actcacccaa ttttagcata
    38641 aaaacaagag gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa
    38701 cagctttcca tgttttctaa ttctttgggc ttattaccga gaagcagcag gtggaacacc
    38761 gtttataaca agaggttgtg acagaagtgg gcaacttcaa gaaataagaa ggaatttacg
    38821 aaaattactt ttcaagtttt tgtgaaattt cggcttattt ttcgaagaag ctacttctgg
    38881 aacaccgttt ataacaagag gttgtaacag aggttgtgac acctgtgtct tgctcatgga
    38941 ggcttatctc gggcatctta ttcctttaat tttacataaa aaggaggctg tgacggtttg
    39001 tctcagcccc tttctattga aatcttgcat tcaaataacg ttctttatct atttatttct
    39061 tgccgtactg ggaacttggt ctaccgtacc accaattacc tagtgtacgt tgtagccatg
    39121 gaattaccca gcgatccaac ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa
    39181 caaaaatgaa ccaaatgttt acccagtaaa acatgcctga taagcagaac gtgatcacca
    39241 atacaatcgt tgtcgcactg catagccatg tgaataatcc agcaatcaat gctaatgcca
    39301 aggcaatttc aaagaatacc atgaattttt ggaagaacat tgctacatca ttgtttggca
    39361 tgatcacttg catgatcttt gtgaaccagt ctggtgactt ctcaatcaca gccattggtt
    39421 cttcaccgta tgcataactc aaaccaaaat gtgcagcttg agtcgctgct tctccggcac
    39481 ttgcccctgc tccgctggca gctgtggttg catcaccagc agcagaagca ccactggcaa
    39541 catctgttac attgtattcc catgtccaag ggaacacact gtttccagtg aaccatgatc
    39601 cttcaccaaa ccaagtactt ggtttgaaca cttcaccatt cccaatgatt tttttggaag
    39661 cttcaacgaa ccaaactaaa ccatagaaaa tacgaagtgg cacactccat aagacattgc
    39721 cgtaacgaga agaatgtcct cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat
    39781 gcattaaata ttggaacata taataaccag aacgaatatc aaagaagtat ttcaaattca
    39841 cgatatgttt cattacgata gctaaaaatc cgcttaaatg gatcttatca aataggttag
    39901 ctacccccca tttggcacca atagaaacca taaatccttg atagtttcct ttaaatttat
    39961 gcttttctcc gcctttgatc gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt
    40021 cagcagcttg aacgatctgc ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat
    40081 caccaatgat atagatgttt ttatcttcgt agccttttgc ctgcatatat tcattggcga
    40141 tcaaccgctg tccgcgtgct gattctaatc caaagtcagc agcgtcagaa gttcctttga
    40201 caccagctgt ccaaatcagt gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat
    40261 gatcagcagc cacttctacg attggtgcat tcaataataa ttccacgttg tgcttacgca
    40321 aatagcgttc agcttttgca gcatcatttc gatccaacat attcaagatc gtcggcattg
    40381 cctctacgac catcagtgtg atttcgtttg gatctaattt ataatctttg gctaggcgat
    40441 ttttccagtc gatcagttcc ccaatcattt caataccggt aaatccagaa ccacagacaa
    40501 cgaaagtcag catggcttta cgcagttcat catctggttc caatgcagct tttgctactg
    40561 tcttctcaat atgttcccga atccttacag aatcttcaaa tgaccaaagg gtgaatccgt
    40621 gtgctttgac accaggtgta ccaaagtcat tcggctctcc gcccattcca aggatcagct
    40681 gatcaaaagg ataggaacct aatttggtct tgacgatttt tcgttcttta tcgataccga
    40741 ttacagtgtc tgtgacaagt tgtacattct tttgacgagc aaacaaatgc tgcagatcgt
    40801 attggattgc ttctggttct acacgtccgc cagctacttc atgcaactcg gtcatcatag
    40861 tatggtacga atgacgatcg atcagagtaa ttgctacatc ttcttttttc agtttctttg
    40921 ccaagtattt tgtagctgaa acaccggcat atcctgctcc tacaacgaca atattttgct
    40981 ttgccattga tcatccgctc cttatagact atctatataa tgaatatatg ataattcttt
    41041 tagtttgata cttattacac aatctccata tattatatag atgttctgtt cttttgtcta
    41101 actccttttg gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa
    41161 agaaaacgtt tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa
    41221 aagattagaa tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg
    41281 gtattaaaat gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc
    41341 tagtgttagg cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat
    41401 ctacagcaca atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg
    41461 gcggagattt gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc
    41521 gtgcagtctt tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg
    41581 tcaatgaaaa cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa
    41641 aatctggcac aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac
    41701 aaagcgcaag cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa
    41761 actacgcaca acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg
    41821 acaatggtgc tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg
    41881 gctaccgtgt gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg
    41941 attatatcga caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga
    42001 aagcaaaaag cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca
    42061 aagcaatggg cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac
    42121 acagctcaca ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg
    42181 acactcaaac aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta
    42241 ataaaagttg gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga
    42301 agagaggaaa aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt
    42361 tatggtgcta actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc
    42421 taccaagata ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg
    42481 tatccgtgtg tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa
    42541 agaacttggt gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga
    42601 gcaagctggg atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc
    42661 ctacgaatat agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca
    42721 attatggcga atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc
    42781 attgaaatta gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct
    42841 aaaagaaaaa ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga
    42901 agtagtgaaa gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa
    42961 tgtctatgtc ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca
    43021 agatccgaac atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt
    43081 agttacctct ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt
    43141 gtttgatcca aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac
    43201 agataaatca attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa
    43261 aggactggaa tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa
    43321 agacgataaa gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc
    43381 ccattacacg atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa
    43441 aatcttttta gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt
    43501 aggggtatta ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt
    43561 ttttattgga atgctagttt ttgatttagc gactaccgct atcaataatt acatggatta
    43621 tgaaaaggct cattctgaag tatataagta tgaagaaaat gtcattggcc gcagaaatct
    43681 atctcctgta ttgattcgaa acatgatttt agctatgatt gcatttgttt ttgttatcgg
    43741 cgttattcta acgatcaata cgggttggct tttacttttg atgggattta tctgttgttt
    43801 tattggagta ttctatacat tcggaccgat tcctttatct cgaatgcctt taggggaaat
    43861 tttcagtggg gtgactatgg gccttggaat ttttgccatg gtcgtttatg tcaatacatt
    43921 cggttcagat gtctatgatc tgacagtcaa cttttcatct ggacaatttg atttggtagg
    43981 aaatctttgg gctatagcag caatcgtatt agcttcctta cccttggtgt ttacgattgc
    44041 caatatcatg ttggctaata atttgcggga tcttgaccgt gatattgaaa atcatcgcta
    44101 tacactagtt ttttatatcg gacgtcctgc gggaacagtc ttattccagc tattgatgta
    44161 tgcttgctat ttcgtgattt taatggggtt gttgagcggt atctatgaat ggccgatctt
    44221 ggtcaccttc ttgaccttgc caattgtgaa gaagcatttg gatcaattca agcaaacgtt
    44281 accccagcct atcagtttca gctattccat caaaaatatg gtcttattca atgccagtta
    44341 tgtattgggg ctattactaa caatcattat gcaagccatt taacagcatg aagaggttgt
    44401 gaaaaaactg ccctgcatca agtaataaga acagccaaat aaaaatagct ccttatattt
    44461 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt atcaggaggt
    44521 tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
    44581 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttatcagagg
    44641 ttgtaaaatc agtgttttgt cctgagtatt agagaaacaa atggaaaaat agttcctcat
    44701 attttgccgt tttttgagct aataccgaag gacagctgat tgaacactat ttgttcggaa
    44761 aaagtgacta actatgacac aatttttttc acagtccctt ttttcacttg atttgattcg
    44821 taccagcgaa cttgacgaga actttcttgg tttggtaact atatggtatt cttttaacat
    44881 cctcgtttgc ttcttctaat ttatttatac aaagtcacac ttttttaaac atctgttctt
    44941 cgatacattt ctatttcttt taaacatcga taccaatatt ttgaacttaa gagtgcatac
    45001 caaatggatt catttactct tcgtcagtta cgaagataat caaaatctcc aacttcccta
    45061 cttatttcgt tttgagaatt atcgaagcaa tcttacataa ataactaaaa aacaaaaaac
    45121 cagaaaatca gtatactgat tttctggttt tacatggttt gttcaataaa aagctgttgg
    45181 aactgttaat attaaattat cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg
    45241 ctactgtatt gtcaaagtct tctttagttg cacccatctt gatagccacg gcaaaacctt
    45301 gcagcatctc atcgacacca acaccaatag catgtaagcc gacgattctt tcttcttttc
    45361 cgacacagat caatttcatt tcgcattttt ggcgataatc atttaatgca aagtacattg
    45421 gggtaaagcg tgaacgataa actttcaatt cattttcgcc atatttttcc aatgcttctt
    45481 cttctgtcaa accaattgtt gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt
    45541 tatagtcaag ataagaatct ttctttccat taaataaacg ttctgataaa cgtctaccag
    45601 ctgcgattgc aacaggagtc agatctaatt taccgatcac atcaccaaca gcaaaaatat
    45661 gttcatccgt tgtattttgg tatttatcta ctttgataaa cccttttttg tccaattcca
    45721 catttgtatt ttccagacct aatttttcgg tattcggtac acgtccaccg gcaaaaagta
    45781 cacaatcacc agttaaggtt tcaccatttt caaatagcac cgtatattcg tcattgtttt
    45841 tagtaatttc tttagcaata tgatgtgcgt aagtttttat tccagcttct tggtacattt
    45901 ggatcaaatt gtccgaaagc attttatcaa acgttctaag tggtcgttct tttcgatatg
    45961 cccacatgac ttcactacct aaaccacgaa atacacctga aagttcagcc gcaatgtatc
    46021 cagcacctaa aacgattgct ctcttaggta actctgttaa ttcaaaaaat ccatttgaat
    46081 caattgcgta ttctccacca ggaatggtca ttttacttgc acggcctcca gtagcgatca
    46141 agattttagg agcacgatag gtttcaccgt ttacttcgat cgtttgacta tcgataaaag
    46201 tagcgtaacc ttttatagcc tcgacatgat tgctatctaa tcctctttga taagcaccgt
    46261 gtagaaaatc aatatatttt tctcgtcttt ctactagacc ggcgaaatca acatttttta
    46321 aatctgcttg gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg
    46381 cttgccacat aactttttta ggtacacaac cgacgttgac acatgttccg cccaattcat
    46441 tcgcttcaat cagcaaaact tttgcgccat gcatccctgc tctgtttgct gaagcaatac
    46501 cgccagagcc gccaccaatt acgatgtaat catatatttg cattttgttc ctccttattg
    46561 aagagatcaa tcaaaacctt tatttaacga tcctttttct acagaaaaca agtgtaacac
    46621 ttttcttgaa ttttgtatgc gaagatgctt aaaacaggga tatatcagct ttttccttat
    46681 ttacgttttc ggatcatatc tccaggtttc accggctgtg cgactggcat agttaaaatc
    46741 atcattggat ttggtgctcg gtcaatggat tcgccttctt cattatgcat aacttcaacg
    46801 gtttgactga aatgactgaa tcctggacca taaaattcga tttcatctcc tacagaaaac
    46861 aagtttctct gacgtatcgt agcaatctgt gttgcctcat cataagataa tacttcaccg
    46921 acaaatttat atactgggat tttacgacgt tcaccgaata attgttcgtt ttcagttggt
    46981 gtattgtagt aaaaacctgt agctaattct cgttgtgcta ctttccataa ctcgtcgatc
    47041 cattcttgct tgcagacata gttttcagga tcttccatat aactgtctac agccgcttta
    47101 tagacatttg acaccgtaga tacataatgg atcgatttca ttcttccttc gatcttgaag
    47161 ctgtctacgc cgttttcaat caattctgga atatgttcga tcattgacat atccactgca
    47221 ctcatcgaga attcttcttc cacttcgcct gtatcagtta aactgtttcg ttcttgacca
    47281 aaaggcatat cgaacaatcc atacttccag cgacaagatt gagaacatcc gccacggttt
    47341 gcatcccgca tcgacatatg gttggacaat gtgcatcgac cagaataaga gatacacatg
    47401 gcaccatgga taaatgcttc gatttcggta tccgtatttt tacgaatctc tgccacttcc
    47461 tccattgaaa cttcacgagc taaaacgaca cgttccaacc cttcttcttt ccagaattcc
    47521 aatgtctcaa aattagttgc tgaagcttga gtggataaat ggatcggcag acctggggct
    47581 tctgtcgcac aaatttcgat caaagctgga tcagaaacaa tcactgcaga aataccgaca
    47641 tcacgcaaac tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg ggtgaccata
    47701 ttagcagcaa cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt
    47761 tcttcataag taaaattacc tgcacggctt cttaaaccat atgcattccc gccaatatat
    47821 actgcatctg ctccataatg gatcgcggtt tttaactttt ccagtgttcc tgctggcgct
    47881 aggatttctg gtcgtttcaa ttttcttttt actgtcattt ctttcatcat actccttatt
    47941 tgatagcaga aggatcaata taaaagaatc ctgtatctaa ttcacggttt tcaggatgca
    48001 atttactgat ttcgctagtc attttttccg ctttttcttg atcccagcta ccttctaaaa
    48061 tttgtttttt tgcttcggcg aatagtcgag tgatgtttac gaatttttcg cctggagtaa
    48121 ataatccgtc caatttccat gtacagtaat gatgctgata aagttcattc aattcattcg
    48181 agagattcag atcattgctg gcaaaaatat gtgtgccatg actatcttca taaatagaat
    48241 aatgtgtctc ttctttttta ggttccgaga taaaaagacc tctttcacga tctttttgtt
    48301 cgtcttgttt cgtatagtta tagtaattct gtagcagtgg acgtttggat tgatggatgc
    48361 atgtagcacc atacacaagg gtttctacag gaatatcgag tttttcttcc atcgccttca
    48421 tctcttcaaa aggaacttca cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga
    48481 aattgatttg acgtgcgctc gttactaatg tttcagcatc ataaatatac ggtatacgca
    48541 attcaggatt ttttttcatc gtataaataa tacctgtatc accaactgtg atcacatcta
    48601 cgtttatttc ttttaaaaaa gccaaatatt caggtacatt tttcattttt tcaggatgaa
    48661 tgatcccatt tacagcaata ttcgcttttt taccatactt gtgtgctaat tctaccaatt
    48721 gtcgctgttc ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc tcttcaccga
    48781 aatatagtgt atctgcacct gcagcaagca actgttctgc ttgttcaata gtttctactg
    48841 tggttatgag ttcgatcata attttctcct cctacaactt cataatagta gcatagctct
    48901 aaaaactttc caatacttct tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa
    48961 atcaaaataa agttcgatat attctgtcca aatataaaaa gagattgtga caaaaatctt
    49021 gtcacaacct cttaatcaag tcttactatt ataaaaattc agctacacag gccaagtaac
    49081 tatagggaac ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat cttacctttt
    49141 gaagcaccta ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc
    49201 aatcgatctt caatttcttt ttgaagtgcg cgacggattg gacgtgcacc atattcagga
    49261 tcaaatccag ctttcccgat cacatcgatt gcagatggtg tgattttcac ttgaatatct
    49321 tgttctttca atcgtttgat aatcgctttg ctcatgattt ggacgattgt atggatttca
    49381 tctttactca atgaatggaa gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg
    49441 aaagctttct ttaactcttc taagatacgt ttttgcatgg ctttgtgatc tttagtaaca
    49501 tcttggacat tgaatccgac atttttttct tctcgaattt gtgtcgctcc gatatttgaa
    49561 gtcataatca tgattgtatt tctaaagtcc acttttctac ctttagaatc tgttaaatga
    49621 ccatcatcca atacttgcag aagtaaattg aaaacgtctg gatgtgcttt ctccacttca
    49681 tctaagagaa tcactgaata tggtttttga cgaatttttt ctgtcaactg tccaccttca
    49741 tcatacccta cgtatcctgg aggagacccg attaatcggc ttgtactgta tttttccata
    49801 aattctgaca tatcaacacg gatcagggca tcttcacttc caaacatgac ttctgaaagt
    49861 gctttggcca attctgtttt tccgacacca gttggtccaa ggaacatgaa tgaaccaatt
    49921 ggacgatcag gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga aactgctttg
    49981 acagcctcat cttgaccaac gacacgttca tgcagaagtc cttccagttc taacaagcgt
    50041 tcgctttctt ttttctccaa ctgctgtaga gggacaccag tccactccga tacaactgta
    50101 gcaacatctt cttctgttac acgatcagaa taaccttgct cctgttttgc ttcaattgca
    50161 gaaacttttt gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg agcagcattt
    50221 tcaaagtctt gtttttggat cgctgcttcc ttttcttgta ctagttcatc gatttctagt
    50281 ttgatcacag ttgattttgt taagtgatcg gtttgatcca aacggacttt cgctgctgat
    50341 tcatcgatca aatcaattgc tttatcgggt agttgacgag aagtgatata acgaacagaa
    50401 agattgactg ctgctcgtac agcttcatct gtgatctcca ctccatggtg ctcttcatat
    50461 cttgtacgta atccttgaag aatcacttct gcttcttcag gagttggctc atctacttga
    50521 atgcgagcaa aacgtcgctc taatgcagaa tctttttcga tatatttttg atattcatct
    50581 agggtcgttg ctccgatcgt ttgtaattct cctcgagcta acgccggttt caagatattc
    50641 gaagcatcaa ttgctccttc tgctccacct gcaccaatta aggtatgcaa ttcatcaatg
    50701 aataagatta cttgaccatc attatagatt tcgtcgatga cctttttcat tcgatcttca
    50761 aattcaccgc gatatttcgt accagcaacc aaagatccca tatcaagcat catcaggcgc
    50821 ttttcttgca tgtcttctgg cacttcacct aaaatgattt tttgagctaa cccttcagca
    50881 atggctgttt tgcctacacc aggttcaccc accagtacag ggttattctt tgtccgtcgg
    50941 ctaagaatct ggataagccg tttcacttct ctcgaacgac caacaactgg gtccagcttg
    51001 ttttcttttg ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg tgttccttgc
    51061 ggtgtttgcc gtttatcttg tacatttaca tttctacgac gattcattcc ttttccagca
    51121 gaatcaccag ctagccccat tttttgtttc agaacttgac gggcttttga aaggctgata
    51181 cctaagttga tcatgattcg agtagccatg atactttcat cttttaataa gctcatcaaa
    51241 atatgttctg ttccaacttt ttgagaacca gttttcttca cttcgctgct agctaaagca
    51301 aaaacttgtt tcattcttgg tgaatatgga agaaagactc ctttagggta agaacggata
    51361 ccaccataac caatcagatg ttcaatttct tcataaattt ccgtttcatt caaatccatt
    51421 tcacgtaata cttttccagc aattccattt tgctcgacca ctaaggctag taaaacatgt
    51481 tctgaactaa ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat ttctaatact
    51541 gcttgggctc tttcagtaaa aagttcttcc atctaatctc acttcccatc ttcataactt
    51601 aaacgttcca gaaatgctcg gagaatccga gcacgtgtat gatcttcata ttcattgaaa
    51661 ttcaatgtgt tttttgcaat tgcactcaac atcaagtttc cttctttttt tgtaattatt
    51721 tggtcttcat aaagtttttg aataatagca aaagcatttt tctcactgat tgtatcatca
    51781 aataactgat ttatctgctc aagtaattgt tttttatctg aaatccttac ttttgcaatt
    51841 cgtatataac caccaccacc acgtttgctt tctactagat aaccacgttg gatggtaaaa
    51901 cgggtattaa tcacatagtt gatttgtgaa ggaacacagt taaagaggtc tgccatttca
    51961 atccttcgaa tctcgatcat ttcactattt tccagaattt tctttatata tgattcaatt
    52021 aaatccgagg tattctgatg tgccatctta ttcattccct ttgactaata ttgaccttaa
    52081 ttataacgaa tttgataata gaaagaaaga agaacgcttt ttgttaaata aatcctttag
    52141 aatcaacaaa aaaaaaatac ggaatatact attccgtatc gacgcgccca agaggagtcg
    52201 aacccctaac cttttgatcc gtagtcaaac actctatcca gttgagctat gggcgcatat
    52261 tcaatttaaa aaaattataa atcatttgaa aacaaaaagc aagataaaaa aaatcgggaa
    52321 gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta
    52381 cttcccgttt aaaaaataaa aaagac
//
LOCUS       DAFBQW010000015        48210 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFBQW010000015 DAFBQW010000000
VERSION     DAFBQW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.015"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LK889_001103"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LK889_001103"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK5095351.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LK889_001104"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LK889_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK5095352.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LK889_001105"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LK889_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK5095353.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LK889_001106"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LK889_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK5095354.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LK889_001107"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LK889_001107"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK5095355.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LK889_001108"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LK889_001108"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK5095356.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="LK889_001109"
     CDS             complement(8053..8523)
                     /locus_tag="LK889_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBK5095357.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="LK889_001110"
     CDS             complement(8569..8994)
                     /locus_tag="LK889_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBK5095358.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="LK889_001111"
     CDS             complement(9061..9330)
                     /locus_tag="LK889_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBK5095359.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="LK889_001112"
     CDS             complement(9341..10936)
                     /locus_tag="LK889_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5095360.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LK889_001113"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LK889_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK5095361.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="LK889_001114"
     CDS             complement(14489..15049)
                     /locus_tag="LK889_001114"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK5095362.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="LK889_001115"
     CDS             15402..16376
                     /locus_tag="LK889_001115"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK5095363.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="LK889_001116"
     CDS             16546..17946
                     /locus_tag="LK889_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBK5095364.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="LK889_001117"
     CDS             complement(18024..18746)
                     /locus_tag="LK889_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5095365.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="LK889_001118"
     CDS             complement(18825..19331)
                     /locus_tag="LK889_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5095366.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="LK889_001119"
     CDS             19454..21253
                     /locus_tag="LK889_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBK5095367.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="LK889_001120"
     CDS             complement(21321..22895)
                     /locus_tag="LK889_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK5095368.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="LK889_001121"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="LK889_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK5095369.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="LK889_001122"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="LK889_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK5095370.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="LK889_001123"
     CDS             complement(24672..25310)
                     /locus_tag="LK889_001123"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBK5095371.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="LK889_001124"
     CDS             complement(25311..25961)
                     /locus_tag="LK889_001124"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK5095372.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="LK889_001125"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="LK889_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBK5095373.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="LK889_001126"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="LK889_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBK5095374.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="LK889_001127"
     CDS             complement(29104..29844)
                     /locus_tag="LK889_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBK5095375.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="LK889_001128"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="LK889_001128"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK5095376.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="LK889_001129"
     CDS             complement(31215..32153)
                     /locus_tag="LK889_001129"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK5095377.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="LK889_001130"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="LK889_001130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK5095378.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="LK889_001131"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="LK889_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK5095379.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKDWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="LK889_001132"
     CDS             complement(35313..35618)
                     /locus_tag="LK889_001132"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK5095380.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="LK889_001133"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="LK889_001133"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK5095381.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="LK889_001134"
     CDS             complement(36724..37008)
                     /locus_tag="LK889_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095382.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="LK889_001135"
     CDS             complement(37026..37916)
                     /locus_tag="LK889_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK5095383.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="LK889_001136"
     CDS             complement(37936..38154)
                     /locus_tag="LK889_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBK5095384.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="LK889_001137"
     CDS             complement(38124..39824)
                     /locus_tag="LK889_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK5095385.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40090..40863
                     /locus_tag="LK889_001138"
     CDS             40090..40863
                     /locus_tag="LK889_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095386.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="LK889_001139"
     CDS             complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="LK889_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBK5095387.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41579..41681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41687..42658)
                     /locus_tag="LK889_001140"
     CDS             complement(41687..42658)
                     /locus_tag="LK889_001140"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK5095388.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="LK889_001141"
     CDS             complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="LK889_001141"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK5095389.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44628..45446)
                     /gene="purR"
                     /locus_tag="LK889_001142"
     CDS             complement(44628..45446)
                     /gene="purR"
                     /locus_tag="LK889_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBK5095390.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45613..46437)
                     /locus_tag="LK889_001143"
     CDS             complement(45613..46437)
                     /locus_tag="LK889_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5095391.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46410..47090)
                     /locus_tag="LK889_001144"
     CDS             complement(46410..47090)
                     /locus_tag="LK889_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5095392.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47113..48051)
                     /locus_tag="LK889_001145"
     CDS             complement(47113..48051)
                     /locus_tag="LK889_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBK5095393.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
BASE COUNT        13201 a        10454 c         8696 g        15859 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttagtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagtcc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctga ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcgcgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgt gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacatccttt aaataaaaaa tacaaaaatc acaaaaatta
    39961 attgaaaatg agagtgtttt caatgaaaac gtgattttgt aatggaattt taaaataaaa
    40021 gaaacaagct tttttaatgc aaatgttgta aaataaaaga gattaagagg aaataagagg
    40081 aggaacatga tgactgagtc agtttatgta catattgata ccacaagtaa tgctgtttta
    40141 acaaaagggc tgactgctac tgactttgca aacagtatcg tgcattttcc tcaaaacttg
    40201 ctcttgcttg atccatctgc ttctgctgga gaatatgaat cccatacagg gctgaaagta
    40261 atccgtggta cagaaaatat tcagcgtttc ttttcctctt ctcgtaacag gcgtggattc
    40321 gatgaattga agtggatcga ctttactgat ttaacgatgc tgaaagaatt gacaccttta
    40381 gaaatttcag aattattata ttttggtcat atgaaaacac atcttcattc gccgtttttt
    40441 tataaactac agaataattt tgtatacttc gatcttaatg atcagctgaa tcggatttat
    40501 tatcgctatt tagatgaatt ttataggatt tttgctagta aattaagttc gatcttgtca
    40561 gataagatca atgtaaaaaa atcttttttc aaaaaagaaa cacctgttga aaaaatcagt
    40621 gtggaattat tgaaagaaat gcgtaatttg atgcaggaag ggatcatttt ttcttttgat
    40681 caagttggtt tgatcgatgg taaatatcgt atcccaatct atgtgattga agattatatt
    40741 tggaaattaa aaggcacccg gtatcgagaa gaggaaatca tcgctacttt aatctactct
    40801 gtcaccgatc gacactggca attggtagaa gatgccgatc tttcttatct gccattgaag
    40861 tagatctatt tcttatcaaa aaataaaaag gaaacaagaa actaggacat gattctgtct
    40921 caagtttctt gcttcttttt tatttacagc tcctgtcgtt tatctggcac aaaaatcgaa
    40981 ggagtcgttt gttggatccc taataaaacg atgatcgtta caatagctgt actcaacccg
    41041 ctggcaccat tcattacgaa agaaaagagc caagaattca tcccccatag tgcatacgct
    41101 ccccagaaaa ccactcctgc aataaaatgc caaaaatatc tggaaagtgc gccaatcaat
    41161 gcaccaaaca cgatgctttt tctaatttgt ttcgtattat cgtctacata gcctttttgg
    41221 acatttgaag ccatgagccc tgctgcgcct gcaaaagtat aggcaatcgg gtactcaatc
    41281 aacacttgaa cgacacttaa ataataaact tgtcccaacg gaaaatgtag gatgccccaa
    41341 atgaaacctg ccagcatccc tgcctttgtc cctcttctta acgcaaaaag agtcaatgga
    41401 atctgcccta atgaaataga aaaactgctg ccgatctgga tcggaatcaa cgacaacacc
    41461 atcgctaacg ctgcaacaat cgttccttct acccaaactc tattttttcc cataaaaaaa
    41521 cttcctcctt tgaataaaaa agtcacaaag gaggaagcag ctttcgaaaa tagtcaaaca
    41581 gctgtttatt cacaattcct acgctcgcat aaccgataca ggtacgaagg gtttagaaat
    41641 agattcaatc tcagcccaat tggactcccc tttgtgagtt tatttattat ttattttttg
    41701 tttcaaacaa tggactaact ggtttgtttt catggatacg tttgattgca tctcccatta
    41761 gtccgccaac acttacttca tcgattttgt cgattttacg atcttctgga agatagatcg
    41821 agtctgtaac aactagacgc tcgatagctg aatcttctat tctttgtaaa gcagggccgg
    41881 ataatactgg atgtgtacat gaagcataca cactagttgc accagcttct ttcaaagcat
    41941 tggcagccaa tgtgatcgta cctgctgtat cgatcatatc atcgatcagt acacaagttt
    42001 tgcctttgac ttgtccaatg atattcatta cttctgcaac atttgctttt ggacggcgtt
    42061 tgtctatgat cgcaatcgga gatttcaaaa attccgctaa tttacgtgcc cgtgtgacac
    42121 caccatggtc tggtgaaaca acgacaacat catcgccttt gattcctttt tctaagaaat
    42181 aattggcaat aagtggcgca cccatcaaat gatcgactgg aatatcaaag aaaccttgga
    42241 tttggactgc atgaaggtcc aaagtcagca ttcttgttgc accagctttt tgaatcatgt
    42301 ttgctactaa tttcgctgtg attggttccc gtgcacgtgc tttacggtct tgacgcgcat
    42361 agccataata aggcatgact acgttgattg tttttgcact tgctcgtttc agtgcgtcaa
    42421 tcatgatcag caactccatc aagttatcat tgactgggct gctcgttgat tgaataacat
    42481 agacatgcga tccacgaata ctttcttcaa tgtttacttg aatttctcca tcactaaatt
    42541 gagtaacaga tgattttcct aactcgacgc cgactgctgc tgcaattttc tctgctaatg
    42601 ggcgattgga atttaacgca aaaattttta atcttgggtc aaagtaatgt tttgacatgg
    42661 ggacctccac tttctttttc acaactctag aattctacct aataaatagt agtcatattt
    42721 tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag ataagggagt ttttttgcat
    42781 atccttcttt attgacttgt cttgctctcg caattgccag cgcatattct ggaatattgt
    42841 ctgtaattgt tgacccggca gcaatcgaac tgtttttagc tacctcgaca ggtccgatga
    42901 tattcgtgct agaaccaata aagctgtggt cgccgacagt cgtatgatgt ttgttctttc
    42961 catcatagtt gacgaagaca acaccacatc cgacattgat ttcttctccc agcgtagcat
    43021 caccaacata agtcaagtga ccgactttag ttcctttgcc gatttgagca tttttcactt
    43081 ccacaaagtt tccgatatgg acgttttcgc caatttcagc ttttggtcgt aaatgtgcat
    43141 aaggtcccac atctgcatgt ttcttgacat ggctgctttc aatgactgag ttttcaatga
    43201 cgacgtgatc ctcaatccta ctgtctacga tttttgaatg tgacccgatg acacaatcag
    43261 aaccaatgac tgtatttcct tggatttgta cacctgcttc gatcaatgtg tctggaccga
    43321 tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac aaagcttacg ccattgacca
    43381 tatgcatctg gttaattcgc tttctcatga tctcatttgc tttagctaac gcgatccgat
    43441 cattgactcc cattgattct tcaaaatcat ccgtttgata agcagcaact gtatggcctt
    43501 cttcttttaa gatttcgatg atatctgtta gatagtattc accttgagca ttgtttgttc
    43561 caactttgct taacgcatcg aacaaagctt cgttgtcaaa acagtaagtt cctgtattga
    43621 tttcttgtac aagagcttct tctggtgttg catctttttg ttccacgatt ttttctacga
    43681 tcccaatatg gtcacggatg attcgtccat atcctgttgg gttttctgct tgtgccgtca
    43741 agatcgttgc acttgcattt tttccttggt gatattcaaa aagattgttc agcgtttcag
    43801 tagtcaataa tggtgtatcg ccactgataa ctaacgttgt tccttttttt ccttccaaga
    43861 aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg ttctgcttgt aacgcatatt
    43921 tactgcgttc tcctagttgt gctttaactt gttctgctcc gtgtccaacg atcgtaatca
    43981 cttcatctgg tttcgtttca ctgacccgat taatgatatg ttctaccata ggtttaccag
    44041 aaacgggatg taaaacttta tataattttg acttcatgcg ggtacctttc cctgccgcca
    44101 aaataattgc ataacgtgcg tccaactgcg tcactcctaa atatagtttg ctgatttcag
    44161 tttatcccag tatacccatt ttttcaacaa aacaagtgat tttcttttat cccagcgttt
    44221 tctggataaa aaaatagata gaccataacg ggcctatcta tttggtaacc ggtgctctcc
    44281 tgccaactcc tcggtcacaa gtcacactac tcataaaaca tgaaaagctg ttttttgaaa
    44341 tcgtttcttg tgacttagag ttaaacggta ggcttacaac ctcttcatta aacgtttttc
    44401 aatcagctga ccttcggtat tagctcaata aacggcaaaa tatgaggagc aatttttccg
    44461 tttattgagc taatactcag gacaggacgc tgatttcaca acctctagat aaacggtgtt
    44521 caaaagccag tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa gccattttca
    44581 caaaattttt cttactactc gaaactaagc ggctttttca caacctctta ttctgaaaaa
    44641 taatttcctg gcacaaccgt aatatttttc gtttgagtat ctacatcttt gacataaagc
    44701 agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc aaatacagtg
    44761 atgccaacaa gttctgattc gaattcttca atcaagctct tcattccgtt gactgttccg
    44821 ccgcctttca tgaaatcatc cactacaagg acacgtgaac cacgtttcaa acttctctta
    44881 gacagttcca tcttttcaat tctttcagaa gatccagaca catagttgac acttactgtt
    44941 gatccttctg ttatttttga gtcccggcgt acaataacga atggcgcatt cagataatag
    45001 gacacagctt gggcaatagg tacgcctttt gtggctacag tcattacagc atcgattttt
    45061 tcaccaaggt atttagaagc aatgattctt ccaacttgac gcagcagttc aggttctcct
    45121 aacaaatcag agagataaac atatcctcct ggaagcagac ggtcttgttc tgatagacgt
    45181 tctgctagag attcaatata ttttttcgcc tcttcatatg aaatttcagg gatgaacaat
    45241 acaccacctg cagcacccgg gattgtttcc aaggttccat accctcgttc tttaaatgtt
    45301 ttcttgatga ttcccaaatc ttcactgata gaagatttag cagattcata acgttcggca
    45361 aagcttgtta aggaaatcaa ttcatgcggg tgattcaata aatactgtgt catgtcaact
    45421 agacgttcac tgcgacgtac tttcacctat tccaactcct tttaagaaat tttatcgttt
    45481 tacattatat aagtttttct tcaaaaattc gagcatttta tgaaaaaata agaaaatagt
    45541 tcggtattta ttaaaataaa aagaagccta taaaaaattt ttcgttttta taaggcttct
    45601 tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt
    45661 tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag
    45721 ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc
    45781 aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat
    45841 tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat
    45901 cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc
    45961 atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac
    46021 gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag
    46081 ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag
    46141 cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt
    46201 tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg
    46261 attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc
    46321 agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc
    46381 aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga
    46441 gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga
    46501 tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga
    46561 taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag
    46621 tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat
    46681 tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca
    46741 tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa
    46801 cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga
    46861 atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct
    46921 tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt
    46981 tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca
    47041 ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct
    47101 ccattctaaa acttaattga tactttcttt taaagctttc agattttttt ccatgacaga
    47161 aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac
    47221 ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc
    47281 ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg
    47341 acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta
    47401 ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc
    47461 ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc
    47521 attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt
    47581 tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga
    47641 atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc
    47701 gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga
    47761 atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc
    47821 tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag
    47881 tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt
    47941 cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc
    48001 aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct
    48061 cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata
    48121 aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta
    48181 aatcgtaaaa tggatgagtt gctgctcata
//
LOCUS       DAFBQW010000016        47996 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFBQW010000016 DAFBQW010000000
VERSION     DAFBQW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.016"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(22..1806)
                     /gene="pyk"
                     /locus_tag="LK889_001146"
     CDS             complement(22..1806)
                     /gene="pyk"
                     /locus_tag="LK889_001146"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK5095394.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(1880..2842)
                     /gene="pfkA"
                     /locus_tag="LK889_001147"
     CDS             complement(1880..2842)
                     /gene="pfkA"
                     /locus_tag="LK889_001147"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK5095395.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(3010..6324)
                     /gene="dnaE"
                     /locus_tag="LK889_001148"
     CDS             complement(3010..6324)
                     /gene="dnaE"
                     /locus_tag="LK889_001148"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK5095396.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            6555..6740
                     /locus_tag="LK889_001149"
     CDS             6555..6740
                     /locus_tag="LK889_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBK5095397.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(6802..7998)
                     /locus_tag="LK889_001150"
     CDS             complement(6802..7998)
                     /locus_tag="LK889_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5095398.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(8168..9604)
                     /locus_tag="LK889_001151"
     CDS             complement(8168..9604)
                     /locus_tag="LK889_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK5095399.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            9897..10706
                     /locus_tag="LK889_001152"
     CDS             9897..10706
                     /locus_tag="LK889_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5095400.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(10892..13045)
                     /locus_tag="LK889_001153"
     CDS             complement(10892..13045)
                     /locus_tag="LK889_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBK5095401.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(13440..14288)
                     /locus_tag="LK889_001154"
     CDS             complement(13440..14288)
                     /locus_tag="LK889_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5095402.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(14512..16452)
                     /gene="thrS"
                     /locus_tag="LK889_001155"
     CDS             complement(14512..16452)
                     /gene="thrS"
                     /locus_tag="LK889_001155"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK5095403.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(16789..16968)
                     /locus_tag="LK889_001156"
     CDS             complement(16789..16968)
                     /locus_tag="LK889_001156"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBK5095404.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(17015..18838)
                     /gene="cadA"
                     /locus_tag="LK889_001157"
     CDS             complement(17015..18838)
                     /gene="cadA"
                     /locus_tag="LK889_001157"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK5095405.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            19116..21395
                     /locus_tag="LK889_001158"
     CDS             19116..21395
                     /locus_tag="LK889_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5095406.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(21488..22135)
                     /locus_tag="LK889_001159"
     CDS             complement(21488..22135)
                     /locus_tag="LK889_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095407.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(22314..23033)
                     /locus_tag="LK889_001160"
     CDS             complement(22314..23033)
                     /locus_tag="LK889_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5095408.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(23164..24315)
                     /locus_tag="LK889_001161"
     CDS             complement(23164..24315)
                     /locus_tag="LK889_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBK5095409.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(24532..25272)
                     /locus_tag="LK889_001162"
     CDS             complement(24532..25272)
                     /locus_tag="LK889_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5095410.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(25276..25599)
                     /gene="rsfS"
                     /locus_tag="LK889_001163"
     CDS             complement(25276..25599)
                     /gene="rsfS"
                     /locus_tag="LK889_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK5095411.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(25656..26249)
                     /gene="yqeK"
                     /locus_tag="LK889_001164"
     CDS             complement(25656..26249)
                     /gene="yqeK"
                     /locus_tag="LK889_001164"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBK5095412.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(26233..26883)
                     /locus_tag="LK889_001165"
     CDS             complement(26233..26883)
                     /locus_tag="LK889_001165"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK5095413.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(26893..27204)
                     /gene="yhbY"
                     /locus_tag="LK889_001166"
     CDS             complement(26893..27204)
                     /gene="yhbY"
                     /locus_tag="LK889_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK5095414.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(27220..28329)
                     /gene="yqeH"
                     /locus_tag="LK889_001167"
     CDS             complement(27220..28329)
                     /gene="yqeH"
                     /locus_tag="LK889_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBK5095415.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(28326..28853)
                     /locus_tag="LK889_001168"
     CDS             complement(28326..28853)
                     /locus_tag="LK889_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBK5095416.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(29033..29818)
                     /locus_tag="LK889_001169"
     CDS             complement(29033..29818)
                     /locus_tag="LK889_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK5095417.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(29811..30680)
                     /locus_tag="LK889_001170"
     CDS             complement(29811..30680)
                     /locus_tag="LK889_001170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBK5095418.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(30681..32060)
                     /locus_tag="LK889_001171"
     CDS             complement(30681..32060)
                     /locus_tag="LK889_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK5095419.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(32068..32487)
                     /gene="fabZ"
                     /locus_tag="LK889_001172"
     CDS             complement(32068..32487)
                     /gene="fabZ"
                     /locus_tag="LK889_001172"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK5095420.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(32512..32988)
                     /gene="accB"
                     /locus_tag="LK889_001173"
     CDS             complement(32512..32988)
                     /gene="accB"
                     /locus_tag="LK889_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK5095421.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(32991..34226)
                     /gene="fabF"
                     /locus_tag="LK889_001174"
     CDS             complement(32991..34226)
                     /gene="fabF"
                     /locus_tag="LK889_001174"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK5095422.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(34245..34982)
                     /gene="fabG"
                     /locus_tag="LK889_001175"
     CDS             complement(34245..34982)
                     /gene="fabG"
                     /locus_tag="LK889_001175"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBK5095423.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(34994..35911)
                     /gene="fabD"
                     /locus_tag="LK889_001176"
     CDS             complement(34994..35911)
                     /gene="fabD"
                     /locus_tag="LK889_001176"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK5095424.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(36018..36242)
                     /locus_tag="LK889_001177"
     CDS             complement(36018..36242)
                     /locus_tag="LK889_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK5095425.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(36295..37257)
                     /locus_tag="LK889_001178"
     CDS             complement(36295..37257)
                     /locus_tag="LK889_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBK5095426.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(37267..37707)
                     /locus_tag="LK889_001179"
     CDS             complement(37267..37707)
                     /locus_tag="LK889_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5095427.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            38013..38978
                     /locus_tag="LK889_001180"
     CDS             38013..38978
                     /locus_tag="LK889_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK5095428.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(39035..39265)
                     /locus_tag="LK889_001181"
     CDS             complement(39035..39265)
                     /locus_tag="LK889_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBK5095429.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(39351..40238)
                     /locus_tag="LK889_001182"
     CDS             complement(39351..40238)
                     /locus_tag="LK889_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK5095430.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(40327..41550)
                     /locus_tag="LK889_001183"
     CDS             complement(40327..41550)
                     /locus_tag="LK889_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5095431.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(41567..42613)
                     /locus_tag="LK889_001184"
     CDS             complement(41567..42613)
                     /locus_tag="LK889_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5095432.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(42777..44504)
                     /gene="ptsP"
                     /locus_tag="LK889_001185"
     CDS             complement(42777..44504)
                     /gene="ptsP"
                     /locus_tag="LK889_001185"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK5095433.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(44504..44770)
                     /locus_tag="LK889_001186"
     CDS             complement(44504..44770)
                     /locus_tag="LK889_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBK5095434.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            45249..47483
                     /locus_tag="LK889_001187"
     CDS             45249..47483
                     /locus_tag="LK889_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5095435.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQKAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            47631..47951
                     /locus_tag="LK889_001188"
     CDS             47631..47951
                     /locus_tag="LK889_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK5095436.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        13691 a        10309 c         8401 g        15595 t
ORIGIN      
        1 tttatttttt attaagctaa attagatagc tgttgtttcg ccacggtaaa cgatgccgcg
       61 acgtgaatca acggtaatca attcaccttc tgtaattgtt gttgttgcgc ctttagcacc
      121 aacgattaca ggaatatctt gtgcaattcc tacaactgca gcgtgagaag tcaatccgcc
      181 ttcttcaacg ataacagcag ctgctttttc gattgcaggc atatattctt tgtcagttga
      241 tggtacaact agaaccattc cgtcttttgc tttttttaca gcttcttcag cagatgaagc
      301 aataactgca tttgcaacga cagatttttg tccaacgcct tgagcttcta ataatttaga
      361 accaatcatt tgaattttca tcacgttagt tgttccgcgt tcaccaactg gtacaccagc
      421 agtgatcaag attaaatcgc cttctgatgc gaaacctaat tcaacagctt ttttagctgc
      481 tagttcaaac atttcatcag ttgttgatgg tttttcagca actgttgggt aaacgcccca
      541 gtttaacatc aaaccacgtt ttgtgcgttc gtcaaaagta acagccaaga tatctgcatc
      601 tggacgatat tttgaaatca tttttgcagt gtatcctgat tcagttgcag caacgattgt
      661 tttcacgcct agattttttg cagcacgagc tacagataaa ccgattgttt cagttacatc
      721 tgttttatcg aattcgttca attggaatgt acctaattct gataatgcat tttcagcttc
      781 ttggtcgata cgtgccattg ttgaaacagc ttcaactggg tagtccccgt ttgcagattc
      841 accagatagc attgttgcat ctgtgccatc aaatacagcg ttagcaacgt cagaagcttc
      901 cgcacgtgtt ggacgtgggt tttcttgcat tgattctaac atttgtgtag cagtgataac
      961 tgggatacca gcagcattac attttttgat aatgcgtttt tgaaccattg gtactagttc
     1021 agctgggatt tcaacaccca tatctccgcg ggcaatcata acaccgtcag aaactttgat
     1081 gatttcgtca atgttgtcga taccttcttg agattcgatt ttagggaaga tttgaacatg
     1141 agtcatgtct ttttcttcta agatttcgcg gatatctaga acgtcttgtg ctttacgtac
     1201 aaaacttgca gcgataaagt cgatatcgtt gtctaaacca aaacgaatat cgtcagcatc
     1261 ttttggagta atacctggca aactaataga aacgcctggt gcatttaccc ctttacgtga
     1321 tcctagcata cctgcatttt taacttctac gactaattca cggtttgctt cgtctttttc
     1381 aatgatttgc atatcgatca aaccatcatc aaataaaatg tgtccgccaa cgtgaacatc
     1441 gtcaaataat cctgggtaag taaccgcgat tttttctttt gaacctacat gttcaggatc
     1501 catagagatg cgtgtttgat ctcctacttc aaatttgatg tatcctgcac gaccaaaatc
     1561 agcttctgtt gtgccttgaa cagttgtacg gatttcagct ccttttgtat ctaaaagaat
     1621 accgacatct tttcctgtct ttttaactgc ttcacggacc atagtcatac gtgataattg
     1681 ttcttcatgg tcaccatgag aaaaattaaa gcggaagaca tttgccccag attcaatcaa
     1741 ttgagaaata atttctactg agttacttgc tggtcctaat gtactaacga ttttggtttt
     1801 tttcatttaa aaaactctcc cattcaattt tgtttattta aagctgtaca cagcttaaat
     1861 agcttggttt ctttcactct taaaatgaaa gtgaattatt tagatcatat agtgataagt
     1921 ctggtttatg tttgttgttt tcaagtgtat caatgatgtc agacgcaacg atttcgttgt
     1981 catggatacc gacacataat ccaccttttc cttcttgaag caattcgaca gcgtaagcac
     2041 cgaatttact tgccaacacg cggtcgcgtg cgcttggtgc tccaccacgg acaacgtgtc
     2101 ccaagataga tacacgtgta tgatagtcac caaattctga aagtttctca gcgaattcat
     2161 ttccgcccat tacaccttca gccaagatga tcaagcaatg tttcttcccg cgatcgcggc
     2221 cttcttggat ttttttagca actgaagcca tatcaaaatc atgttctggg atgatgattt
     2281 catccgctcc gccagccaca cctgaccata aagcgatgtc tcctgcattg cgtcccatta
     2341 cttcgataac gaatgtacga acatgagaag tcgctgtgtc acgaatacgg tcaattgatt
     2401 ctagtactgt attgattgct gtatcaaaac cgattgtgaa atcagtccca ggaatatcat
     2461 tatcgattgt tccaggaata cctactgctg ggaaaccgcg ttttgtcaaa gccattgcgc
     2521 catgataaga gccgtctcct ccgataacca ctagaccttc gattccgaat tttttcaatt
     2581 gttcgattcc ttttaattga ccttcttcag ttgcaaattc tggataacgt gcagaataca
     2641 agaatgtacc accacgttgg atcttgtcac ctacatctgc cacatcaaga cgacgaatat
     2701 ctccagcaac taaaccggca aaaccataat tgatcccgta tacttccagt ccttcgtaaa
     2761 tacctttacg aacaacggca cgaacagctg cattcattcc aggtgcgtct ccgccactgg
     2821 tcaaaattcc gatgcgtttc ataataaaca ttcacctcat taaaatactt tatcctaagt
     2881 atgacttagg tttacaacca tttttgcgtt aaccacgact aacattctaa cattattttt
     2941 ctcacttgtc ttgatttacg gataaaaaaa tgaaaaaaaa tgaaagcaag tagtattttt
     3001 taccgtaagt tatttgaaaa ccacattatc ttcgccaagc agataaataa gttgcccctt
     3061 tatactcttg ctgtcatcca cccaataagc atctgacaga actacttttc gttgcgtttt
     3121 ttcgtgatac ataactactg gtatatatcc tgggtgtttc tgaaaaatca cttgtaattg
     3181 tttcattatc tcgggatctt cccgttcttc tgtaatacgc aaatagcaag tttgtgaagg
     3241 atactgggct tctaactggt taggatcagc aaagctttct ccaattagtt gcagttcctg
     3301 attatatttg ctgatttcgc tccgcccttg aacaaagatc actcgatcaa cggcaaacaa
     3361 attacgatat ttacgataaa gagttggaaa aactgtgaca gaaatttctc cactactgtc
     3421 ggttccttca acaaaggcca tcagttctcc tttttttgtc cgaatttctc taacttcctt
     3481 gatcatcact aataaattga ctggttgatt agggacgata tctgtaatca gctgaacttg
     3541 tttagctaaa cgaagacgat caaacccttc tgcaggatga cctgacaaat aaatacctaa
     3601 gtactcctct tccagcttca agcgttcttc tagtgtatag tcagcgactt cttcttcttt
     3661 taatgccatg attcccagca agtcaagact tccaccactg taaacgatat tttggatttt
     3721 tccttcgaga tcaagcatca attgtttcct gttaggagat aattcatcaa aaactcctac
     3781 tgctgtcagt gattgcagta actctaattt cagccaacgc ttatcgatcc gtatgaggaa
     3841 ttgatccact gactggaacg ggccattttg ttttctttcg tttaatatat cttctacaaa
     3901 atcacgccga atacctttta tcgctgtcag tccgaatctg atttcttcct tattgaccaa
     3961 ctcaaaactg gaattacttt gattaatgga cggcgaggaa agtttgagtt tatttttacg
     4021 tgcttctgca atatattctt tcaattttga tgtgttatgc cgaacagaat tcatcaggct
     4081 ggtaaagaaa gcacctggat gatgggcttt tagatacgcc atttggaatc ccacgaatga
     4141 ataagcaaat gcatgtgacc gattgaatcc atagtttgca aatttttcga tatattcata
     4201 tacggtcgct gctgtttttt ccgaatatcc ctgttgtaat gctcctgata caaaatgcga
     4261 cttttcttca tctaatacgt cttttttctt tttactcact gcccgtcgca gaatatctga
     4321 ctgccctaat gtaaatccag ccatagtcga ggcgatctgc atgatttgtt cctgatagac
     4381 gatcactcca tatgtgtttt tcaaaatcgg gatcagtgaa tcatctgggt agcgaatggc
     4441 ttcttttcct ttcttcctgg caatgaaggt atctatattt tgcatagggc ctggtcgata
     4501 caatgcattg actgcggcaa tatcttcgat gttttctgga tctaatcttc gtaaaacgtt
     4561 ccggatacca gcagattcaa attgaaatac tcctgaggtt tcgcctcgtt gaaacaagag
     4621 cagcgttttt gcatcattta gcgggatctc ttttaatcgg atctcatacc cttctactcg
     4681 cttaacagct ttcaaagtaa aatcaataat cgataagttg cgtagtccta aaaagtccat
     4741 tttcaaaaga ccgattgctt ctacctcatt catcgtatac tgggtcagaa aagcttcatc
     4801 agaaccgcgt tggagcggaa ctagttcaag tagattctta tcgctgataa ctacacctgc
     4861 tgcgtgagtc gaaacatgtc ttggcaatcc ttctaaaatc actgcagttt tgaataaaag
     4921 ttggtttttt tcgcttgcct gaactaattc tactaattga tgcgaagttt tataagcttg
     4981 ggtcaatgtg attttcaatt gattgggtat tgcttttgac cagcggtttg cttcactttg
     5041 cgataaacca aacactctag ctgcatctct aagaaccatt tttgctgcca ttgtgccaaa
     5101 tgttgcgatt tgtgccatat gatattgtcc atacttttct cgaacataat tcaatacttc
     5161 ttcacgtcga ttgtcaggaa tatccaaatc gatatctggc atcgtaaagc gttcggggtt
     5221 taaaaagcgt tcgaacagca aatcatattc taatggatcc acatctgtga tttctaacac
     5281 ataggctact aatgaacccg cagcagaacc acgacctgca ccggtaacga ttttgtttct
     5341 atgagcaaaa gccattacat cccatacgat caagaaataa tcatcgaatc ccatttgatg
     5401 gatgacagat aactcgtagt ccaaacgttc ttggtattcc tttgtatagt cagtgattcg
     5461 gcgttccact tgcgaatagc ataactctct taaatattct ccagcttgtt ttccattagg
     5521 aactggataa tgcggaagta atttctgatg gaactggaat tcaaatgaaa tcgattcagc
     5581 taaacgctgg gcgcctatta cagcttggga aagacccgct ttttcgaatc gtttgatcat
     5641 ttcttgttct gaaagaagat agacatttgc tgttagtggt aaagatgcag ataaattttc
     5701 taaaggtatt tggcttccct ctttgatatg tttcatcact tcaagtgcaa aaagttcttc
     5761 ttgttgtaaa ctagcgatgg gatgtaatgc acaaagcgaa taattttctt ttatcacata
     5821 ctgataccat tcaggcaatt gatcaggcgt gttttctgtc aagccgatat aaaaatttct
     5881 agtaaaaaca ttttcaagta aaaactgcca atgaccgaaa aagttatcct ctttattgaa
     5941 agcctcagaa acagaatgat ttgccggaac aacaaacgct agatggtcgg tttctgtcat
     6001 ttctgcaagt tccaattttt cttggatcat ttttcggctg gaaagtttca tcagctgctg
     6061 ataacctatt tcatcttttg cgtacgcaag caagccataa tttttttctt cataagtata
     6121 actaagatgt aatccaatga ttggctgtac tccttcttct acacaaatac gatagaattc
     6181 aggtgcaccg tataagacat ctttatccgt tatggctaat gaagtgtagc cttgtttttt
     6241 tgcttcttgt acatattctc gaatacgtat cgtactttgt aaaagagaat aagaagtaat
     6301 tgtataaagt tgtgggaaaa acatcttgca ttccgccttt tttttcgtta aaattcacta
     6361 gcgttaaaag gtttgatcct tttactttca ttaagatagc aggcgctgaa gttgattatt
     6421 ccacttcatt tgcttgcttg taatctgtag gaatgagttc tttcgcaaga actcaaattt
     6481 tattgagatt tggtctcgtt tctttacatt caaagaaaat ctggtaaaat taagatagaa
     6541 aagaggtgtt ctttatgcga tatatcgtta cattattctg ggctgtcgtt ctaggtcaag
     6601 tagtcggtta tattggtgct gcattaacaa gcggaacata tgattttact ttgacaacaa
     6661 tcatttcatt cattgcgggt gtcattatcc ttttgattgg tgcagttgcg ccaagaaaag
     6721 aaacctctgc ccattcgtaa aaaaacagcc aagagcttga gatttctcaa gctctttttt
     6781 attttctact cctcaccacc attataatct gctgcgaaga actttacgaa agttgaataa
     6841 agaaagaaga atatttgaca gaacaaacaa acttgtcaca ataaaaacag cactgtaatt
     6901 ataagcatgc gcgactgtcg atcctagcat gggacctaaa acttgtccaa agttgctaaa
     6961 catctgattg tagctataga ttcggctgac tccttcttga ggcgtaatct tactgatcag
     7021 tgtattgaca gaaggcatca aagcgccagt tgaaaagcct aaaataaagc gaagtatacc
     7081 taattggagc ggtgtagtga cgaatgccat aggtaaataa cagcaaaatg ataaaatgag
     7141 tcctccaatt agaacttttt gattcccaat cttatcccct agttttccta gtttcggtga
     7201 agaaaagaca gcggaaacac cagctatcga aacaatcaag ccgctgacaa aaaggatatt
     7261 ttctgtactt ccgctcaatt cacgaatata taaggtcaaa atagggctga tcgtagtgat
     7321 tcctatctgt aaaatcaagg tcgtaatgaa gagaccgatc aaaatagaga gatgatccat
     7381 ttttgaaaaa atttctttgg tgcttatcag atcttttttc tcgattggat ggaaatcttc
     7441 tttgactaag aaaatggtca ataccgttgt gatcatcaac aaagcccctg tgatcaagaa
     7501 tacattttcc ataccaaacc actgagccag tgcaccgccc attgaaggtc cgatcagatt
     7561 tccagcaatc gctccggtag acaatgttcc taacgcccat ccgctctttt ctctaggtgc
     7621 ctgtgaagca atcatcgctg tggcatttgg aatatatcca gacaaaatcc cattccaaaa
     7681 ccgcatgatc aaaagccaat acgcgtttgg tacaaaagcc aaagatccca ttgttaatgt
     7741 catccctgct gctgcacgga tcatcatgag ttttcggcct tttcgatcag ctagatttcc
     7801 ccagattggt gcaaccacgg cagatgcaaa agcagtcaca gagattgcta gtcccgaaaa
     7861 caattcgatt tgatcttttg gtgttcctag ttgctcaaca taaactggta tgaatggcat
     7921 aactaaacta atacttgcac cagtgaaaaa acagccaatc cacgcaatca ctaaattttt
     7981 tctccaatta atctccatct atcatccctc tttcgtctct tttatcatac ctattttcga
     8041 ataaacaagc aacgatctcg ccagtaaaaa gagaaaaatc ccgcaaaggt cattttatat
     8101 gtaacgttct ttttatgtaa aacaaaaaga gatcagaaca aatactgttc caatcccttc
     8161 tctttagtta aattgttgct agaatgatga agttgataat gaataaaata tcaacgatcc
     8221 acaaagcaac agaaacttga ttgtattttc ctttgattac tttcacaatt gtatagaaaa
     8281 taaatcctgc tgcaatccca taggaaatgc tatagcacaa tcccataaag atcgatgcaa
     8341 agaaggccgg taaagcttct tccatatcta gccatttgat gtctgcaaaa gaagccatca
     8401 tcattactcc aactaaaatc aatgctggcg cagttgcttg atttggtaca atggcgatca
     8461 gtggagagaa taaactactt aatgcgaata gcactgccac tactactgaa gtcaaaccag
     8521 tacgtccacc ggccccgatc ccagctgcag attcgacata agttgttgta ttcgatgtac
     8581 caaagatagc ccccacagaa gtagcgatcg catcggcaaa tagtgcttta tccatttttg
     8641 ttttgaaccc acgaccatct tccaacgcga tctcgtcttc ttttgagaaa atacctgtgc
     8701 ggcgtcctgt accgataaat gttccgatcg tatcgaaagt atcagataaa ctgaatgcga
     8761 tgatcgtcat caatacttga ggaatcttgg aagaatcact gaataaagat tgcatgcctt
     8821 cagaaccaaa tgctgcaccg aaagttgttc ctaattcttt aaatgaatta cctaacgaat
     8881 tcgtttgcca atcaatggaa cttaaatcaa caacacccat aaaaatacca aggactgtgg
     8941 tcgctacgat accgataaga atagccccac ggacattttt tacaactaaa atggctgtca
     9001 gtaccaaacc aatcacagcc aataagatag gagcgttgtc aaagttggct aacgcaggga
     9061 cgattccacc atttatcgta acatttgtcg cttttccgcc ttcaacgaca gagctcgaaa
     9121 tagctgactg gtctgctgaa aaagataaga atcctgcatt tttgattccg acatacgcaa
     9181 cgaaaatacc gatcccgcca ccaatcgcat gttgaagact ttcgggaatc gcccgaataa
     9241 tcatttttcg aatatttgtg acagtaataa aaatattgat taatccgcaa ataaacacca
     9301 ttgccaaggc ttgttgccaa gaataaccaa gcccaaaaac gaccgtaaaa gtgaaaaatg
     9361 cgttcaatcc cataccaggt gcctgagcat atggtacatt tgcgaacaat cccattacca
     9421 gcgtaccaat aatcgatgca atgattgtcg ctagaaatac tgcttggaag ggcatacctg
     9481 atgctgacaa aatcgtcgga tttacgaata agatataact catggcaaag aatgtcgtta
     9541 ctcccgccat tatttcagtt gatacatttg tattgttttt ctttaaaccg aaaaactttt
     9601 ccataaaaag ttccactccc tgaatttttt tctgatggaa aactataata ataaaaaata
     9661 agtaaaatag ggcaaatcca tcgaacaatt tcaaattata tagacgtttc attcgctttt
     9721 caacgttttt ttaacaaaaa aagcgaaaat aatacttaac gttcgtgttt agcttatgtt
     9781 tagctattta tatagccata actgtagttt ttgttaggaa tagctgattg ctcttgttaa
     9841 ttacctaaaa tatgctagac taaaacaaat gctagttgag gaaggaagga cccttattga
     9901 ataaaaaatt aattgccatc gatctggacg gaactaccct caatcaagat tcgatgatta
     9961 catctaaaac aaaagagact ctaaaaaaag caatcaatgc cggtcatcat gtcagtattg
    10021 ctacaggacg cccattccga atgagccatc agttttacca acaattagaa ttaacaacac
    10081 cgatggttaa ttttaacggc gcattagtac acatccctaa tcagcactgg gatggtgaaa
    10141 gagaaacgtt gatcaatcgt gaaatcgctt ttgaaatctt atcgcaaaaa aagcagctaa
    10201 atttagattt tatcgctgct gaaaatcgtg atactttttt tattgacagt tttgatttct
    10261 ttgatgaaaa aatttttgct tcttctcgtc caggagagaa gaatttgctt tctcctaaaa
    10321 atttgacgac aaatcctaca tctttgcttg tccgaacaga caaacgtttt gcaggagctg
    10381 tatctgctga attaacacgt caatttggca gttatgtcga tgttcggaca tggggtggac
    10441 ctactgccat cttagagatt gtttcaaaag gaatccaaaa agcaaaaggc gtgcaagaga
    10501 ttgctaacta cctgtctatc gatcaaaaag acgtcattgc ctttggtgac gagcataatg
    10561 atttggaatt gttggattat gcaggttggg gagtagctat ggctaacggt actgaccaac
    10621 tcaaaggcat tgcaaatgat gtcactcctt tgagcaatca ggaagacggt ttagctgttt
    10681 atctggaaaa actacttaaa ctataataga gaattagatt ccacaaaaaa gaggttgtga
    10741 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
    10801 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
    10861 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
    10921 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
    10981 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
    11041 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
    11101 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
    11161 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
    11221 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
    11281 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
    11341 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
    11401 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
    11461 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
    11521 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
    11581 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
    11641 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
    11701 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
    11761 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
    11821 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
    11881 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
    11941 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
    12001 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
    12061 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
    12121 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
    12181 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
    12241 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
    12301 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
    12361 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
    12421 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
    12481 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
    12541 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
    12601 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
    12661 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
    12721 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
    12781 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
    12841 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
    12901 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
    12961 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
    13021 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
    13081 ttgtacccaa aaacttctta ataaacaaga agtatttaat atttttttca taaaatcttt
    13141 catcatttcc tatccttccg cctatttaca ttcatgcttc attgtaccca aaaacttctt
    13201 aataaaaaag aaaaaatggg cggagcaaga aattctttac ctaatttcaa gataaaatca
    13261 actttttgtt tcacagcaat cacttaaagt tacatatcca actatttttt tcattaaaaa
    13321 ataaacttat ttttgaaaat tatgttttct taagtcgtga aacactttga tggatcattt
    13381 atttgtggaa aactacgttt tgttattcta aaaaacttat ataaaaaata gtaaggtttt
    13441 tatttcgaat ccataaaata ttcttggatt gcttctcgtg tttttttcac tttttcgcta
    13501 agcttttcgt ttttagccat atagaaataa aaaatgatat cgatcgtagc aaattgggcc
    13561 aaaagtgaat tagttgccgc acttcgattc gtgatttctt ttggtctaga tgtttgtacg
    13621 agtacatcgg ctatcttgct aagcgggtta tttccaaatt gcgtcaacgc aatgatttcg
    13681 atattcatgc ttttcgccag ctcggctaat gcgactacat ctgcacttct acctgagttt
    13741 gaaacaagcc aaagcacttt ctttttctca ttgctgacaa gttgcgggag caagacgtgg
    13801 atatcttttt caaaaatgat tggcttaccg acacgagacc atttttgcag aatatcttct
    13861 actactaaag aagaggcgcc aaccccatat aaaaaaacag tctctgccga ttccaaacac
    13921 tctacgactt tttggatcgt ttcttcttct agaatatcta ctgtttcttt tatcgctact
    13981 tgtgcattgt taccaagttt gtttttgata gagtaaaagg actcatttgc ttctatatca
    14041 ctaaaacctt catcgattcc agtctgtagt tctgttgaaa tcgtgatttt gaagtccgtc
    14101 agactttgaa aaccgatttt tttggaaaaa cgtacaactg tcggcgcact caacccggaa
    14161 gcttctgcta attcattagc tgtcattgta gggaccttct caatattacc gataatgtag
    14221 ttggccaact ttttttctgc tgaagaataa tcttccatca tactttcaat acgactaatt
    14281 acgctcataa gttcctccac ccttttaatg atagtctatg taagtatacc atatcagacc
    14341 cttattcacg gtcgtatact ggataaaaaa caaaaaaaca atccgcccaa cagaatatct
    14401 tatcccatca atcccacaga ttctattgag catctgtgtc tttgaggaat acctgttatc
    14461 tattgttcgg attgctttct atctactact tttttacagg aaatttacct attagttttc
    14521 tctgctgtaa tttttgactt ctgcttccat agcatcaata aagatattca aatcttctac
    14581 agctgtttct ttacttccat aacgacgtac gttcactgtt gcatcttcca attccttgtc
    14641 tccaacaact aattggtaag ggatcttttg tgtttgagag gcacgtatct tatagcccat
    14701 tttttcatta cgatcatcaa cttctacacg caaccccttc atttgcaaac gttctttgat
    14761 ttcataagca taatcgccat gtgcatcaac agaaacaggg atgattgttg cttgtatagg
    14821 tgccaaccat gttggaaaag cgcctttgta tacttctgtt aaataagcga caaaacgttc
    14881 cattgtagaa acaattccgc ggtggataac tactggacga tgcgtatttt ccccgtcttc
    14941 tccaacatat gtcaaatcaa agcgctctgg caataagaag tctaattgta tagttgataa
    15001 tgtttcttct gtaccaagag ctgttttgac ttgaacatct agttttggtc cgtaaaaggc
    15061 tgcctcgcct tctgcttcaa agtaatctag ttctaattca tccatagcgg ctttcagcat
    15121 tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat ttatctgtat tgtttggatc
    15181 gcgatagctc aagcggaaac gataatctgt aatattgaag tcagcatata ctgccaccat
    15241 caattcaagc gtacgtttaa attcatcttt gatttgatca ggacgaacaa atgtatgacc
    15301 atcattcaac gtcatttctc gtacacgttg taaacctgac aaagcacctg atttttcata
    15361 gcgatgcatc attcctaatt cagcgatacg aattggtaat tcacgataag aatggatcgt
    15421 gtttttgtag accatcatgt gatgagggca gttcattggg cgaagaacaa gcatttcgcc
    15481 atctcccata tccattggcg ggaacatgtc ttcttgataa tgatcccagt gtccagatgt
    15541 tttgtacaac tcaacatctc ccattattgg tgtatataca tgttggtaac ctagactaac
    15601 ttctttgtct acgatataac gttcgatcgt gcgtcggatt gttgcccctt ttggaagcca
    15661 gaatggcaaa ccagaaccta cttctgggga aaccatgaac aagtcaagtt cttttcctag
    15721 tttacgatgg tctctttctt ttgcttcttc acggagacgg atatattctt tcaaagcttt
    15781 cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt tgattgtttg agtttccacg
    15841 ccaataagca cctgccactg ataataattt aaatacttgt atacggccag tggatggaac
    15901 atgtggtcca cgacacaaat cgatgaattc gccttgttga taagcagtaa tcacttcttc
    15961 ttctggcaat tcactaatca gttctacttt gtacggatca cttgcaaaaa tttctaatgc
    16021 ttcctgtttt gaaagaacac ggcgttcgat cggcagattt tctttgacga ttttcatcat
    16081 ttctgcttcg attgccggca aatcttctgc agttacttgg ttttgtccat tatctgtatc
    16141 gtagtaaaac cctgaatcga tagctggtcc aacgccaaag tggatatttg gatagaggcg
    16201 acgcatggct tgggccatca aatgagctgc tgaatgacgg actaagccaa gtgcatcttc
    16261 atggtctggt gtgatgatct caatacttgc atcttcctcg attggacgag tcaaatcaac
    16321 gagttttccg ttcactttac cagctaaagc tttttttgct aagctttttg aaatgctttc
    16381 agcaattgcc aatgttgttg ttccagactc aaattctttg actgcaccat ctggaaaagt
    16441 aatttttatc ataatagttt cctccttgaa atgttttgat tgatcagatc atcaagctga
    16501 aaaacaaaaa aatcctagca tctcttgaaa agagatacta ggacgaaaag ttcgtggttc
    16561 cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt aacggtccga ccgcccttgc
    16621 ttcaacaagg gtttcgaaag tggtcatcct ttggttttct cctggaaaag tttcagccta
    16681 gcttttcctc tcttatcgaa gactctcaaa ttcagttggc tttctcatca atcaattatg
    16741 tgtttattta accactaata aatagaaaaa tcaagtggga aactaaactt attttttcca
    16801 ttccccattt acaccgtatg ttcctttttt catctcattg atgatgacat ggatgtgttc
    16861 ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata tctttcatca tattttctaa
    16921 ttgttccgga ctgcgacctt ctactaattc gacatgtacg aatggcataa tgctctctcc
    16981 tctttctgat cgttcttttt tcaactcgat tctatcatac tatgtttgtc ttgggtactg
    17041 aatcaggaaa aatggctgga tctttttttg ctctcaaggc actcaaaata gagaccgtat
    17101 ccacgacttc ttgcaaaaca gctccgaata atgcggggat tagtcccgta cttgcaatta
    17161 acatcaaaaa aatacaaata aagatgccaa tcagtacaga ctgtttcgct attctcatcg
    17221 tatctcttga aatcactact gcttttgcta cacgcaagag atcatctttc aagatgaccg
    17281 catctgcact ttcactagca gctgtagaac catgagcccc cattgcaata ccgatatctg
    17341 cagcagcaag tgctggtgcg tcattcacgc cgtctcctac catcgcaaca ggtctttgat
    17401 ctttagggat actttctaaa atggtcagtt tatcttgagg caagctacgg gcatgtacag
    17461 aatctaattg gactttttct gcaatcgttt gagcaatggc agatccatcc ccggtgatca
    17521 tcatcaaatt tttcacatca agttttctta gctccttgat tgtctttgct gcttctgggc
    17581 gaatctgatc cgaaaatagg atcgttcctg caaaagatcg atcaattgtt acatagattg
    17641 ctgtattgga cgtttctatt tcttcagctg aaacaaaaga ggcacgtcca atctttactt
    17701 catgattatt gactactgct tggattcctt gaccggtcac ttctttcagt tgactaacag
    17761 gatacagttg gctgtttttc acgttgttca ctaacgaacg agctaagata tggctagatt
    17821 cctgttctgc actagctgca tacagaagta gctcttcttt ttttattcca ttgactggct
    17881 gaatcgcctc tacttccaat atccctttgg taagcgttcc tgttttatca aaagcaatcg
    17941 tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt tacaacgatc ccgtttttac
    18001 ttgaacggct cattcctgca actaaagcaa tgggcgcagc tagaatcagt ggacaaggtg
    18061 atgccactac cagtacctca gcaaatcgta aagggtcttt tgagaaccac caagaaatac
    18121 caccgatcaa ataagcaatc aacgtgaaag ggactgcata acgatcagct aaacggacaa
    18181 atcgggcagg cttttctttt gattcttcga ctagtttaat aatcatttga tactggctgt
    18241 ctgcggcacg tttttcagcg atcatttgga tcgatccgtc tccatttaaa gaccctgaca
    18301 taatctgatc tccaggtttc ttggtttccg ggcgggactc acctgtcaaa gaagattcat
    18361 cgaccgaaga ttctcctttg gtcactcgac catctgcggg tacaagttcg ccaggcttga
    18421 ctagtaactg gtcatcaata tttatttcat cgattactat atcctgcaat tttccttctt
    18481 tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga ctgcagttct ttacccgctc
    18541 gtttattcgc gtaatcctct agactatctc cgcccgtaag catgatcaat atcatcaaac
    18601 tagcccagta ttctttgacg gctaatgttg caaggattgc ggtaatcgct aatacatcta
    18661 cgccatatct tcctgaacgt aacgtcttga tcatttccaa aaacatcaag aaggcgagaa
    18721 tccctccagt aaatgcgaca atcgcaaatg caagggccgg ctggtgaaaa acaaattcac
    18781 ttaataaagc tatcccacca ataagcaaag taagtaaaaa ttttttgaaa tgtgtcattg
    18841 ttttttctcc tcttttatgt gacttagaga ggttagtaat aaatttccag ctgcctcttt
    18901 catcttaagc atagtccttg actagcactt tttaaaacaa taggccttaa ttgaaaacaa
    18961 ctatcactat cagcgtttcc aaaattcatc agttctacaa acaaaaatat tcagcaagga
    19021 ttactttttc gattcatcag ttaaaaagta ccttttttcg aaaaactatg ctatactttt
    19081 aattcgttat ttacggcgga aggagcaaac atgcgatgaa tgagaaacaa ctagaacaaa
    19141 cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca aaatatgctg gatacaaaac
    19201 aacaagaatt gactcaggaa atgaatcatt cgacaaaaga cacagctaat cacaagattc
    19261 gagctggttc caatgaagct ttttatgaat ctgctgttga atatcgccaa catgagcagg
    19321 aattgctttt gaaataccat actcttgaag cacagcaaaa acgcctgcaa acgttgtctg
    19381 tcatgaaaga tagtccttat tttgcccgta tcgactttaa agaagaccgt gaaacagaaa
    19441 cattatatct aggtatcgct tctttaagag atctggcaga agaaacaatc gttattgact
    19501 ggcgcgcacc aattgccaat ctttattatg aaggtgagat tggtcccgca ttttatgaaa
    19561 ctgaaacaga tagaatagat gtcgatctgc agttgaaacg acaatttaaa atccaagaag
    19621 gacgtatttt atctatggta gatacgtcgg aagtaatcaa tgatgacttt ttgctagaga
    19681 tattggatga atcatcaagc aaccatatga aaaacatcgt ctctacgata caaaaagcac
    19741 aaaatgcaat cattcgggac acaacaagta aagtaatgct gattgaagga atcgccggaa
    19801 gtggaaagac atccgctttg cttcaacgta ttgcttttct gttgtatcat aatcgcaagt
    19861 ggctcgaggc tgaaaacgtt cttctatttt cacccaacca tttgttttct gattacatct
    19921 cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac acagacattc aaaaattatt
    19981 tggtgcagct gctgccagag ttttctttac tagatgaaca gcaacaagaa agtggttttt
    20041 tgctagggga aaaagatccg atacaagtga tgaaaagcgg attgacctta gtcgatcaga
    20101 ttgatcgcta tatccaaggc atcacaaact acggtccctt gttccgtgat atgaaaataa
    20161 atggccgtac gatcctatca aaggaatcga ttcgccaatg gtataaagaa acaaatgaac
    20221 aattaccttt acatcataga ctttctctat tacaaacgaa gctcctgaaa aaattaggcg
    20281 gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga tctggcggaa gaacagctcc
    20341 aagaactata cgcaacagat ccaaatcttg aatatacaga acaaaaggag cgttcattac
    20401 gtaaaaaact ggccaaccaa atcgtacgta aaagattccg tcgaatccat cgagggatca
    20461 tgaattatca atttgtcaat ataacgaaac aatatctcca ctttctacaa actgtgccac
    20521 tttctcttct aacacgacat aaaatcagcg agaccaggtg gcaggaacat ttgacaactg
    20581 ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga tgctgtacta ttctttttac
    20641 tgatgcgccg aacatatcca gtgacggtta cacacaaagc tcgctatatc tttattgatg
    20701 aaatgcagga tttcgcacca tcacaagtgg cattgctgca agccttgttc ccaaaagcaa
    20761 atttgacttt ctgtggagat ctaaatcaaa atgtctttgg aaatgaaacg atcgttggat
    20821 cattggaaga ccttttcgtg gaccgtgaag tcactcgttt ccaattaacg acaagctatc
    20881 gctccacaaa agaaatcact gattttgcta atcattttat tgtgaaagaa catcaagtag
    20941 aaacaacagc aagaaaagga gaccttcctt tgctcatcca aggaacaaca gatgatatca
    21001 aattgaattg gttagcagat accattcgaa cgactcaggg aaaagcccgg tattggcgta
    21061 cagcaatcat agctaaaaca caagaagaat gcgaagctct ttatgctcaa ctgccagacg
    21121 agttgaaaca acaagtccag ctgattttaa atgagagtga ctttatgaag cgttccatta
    21181 ttttacttcc agccttttta gcaaaaggtc tggagtttga ccgagttttc ttgtgggatg
    21241 tagatgcaga acatttttct tctcagcaag acaaattgat cctttacacc atgtgtacac
    21301 gtgcgatgca tgaattgacc ctttttacct ccaataatga aagtcctttg ttaaacaata
    21361 tggatatttc atcttatgag aaaaaagtac tgtaagtaaa aagacaactg ctatcattaa
    21421 tgttagcagt tgtcttttta tttacaataa taattgcaga aaaagtaatt caatcattta
    21481 aggtcattta ttctatatat aaatccaaat ctgtttcagg atattttaat ttgacaagcg
    21541 aaataatcat tttcagttct tccatgtttt ctggatattc tgggtcaaat accttgctat
    21601 gacggccaat cccgccattg tcgatcaatc ggtaaattcg attccattca atgaattggt
    21661 caattgattg gaaattgctg attggatagc cgccacaatc caaaatctct gatacacgaa
    21721 atgctaggta tgcactatcg atcactttat tttttttcgt gtctatcttc tcatttcttt
    21781 cgataagaat tttttcagga cgatgtttca agctccactc taatagtaga aagaaagcct
    21841 ccatccgata ctcttcttca aataaccgaa aggctgaatc agaacgacga aaaagcagcg
    21901 gaatcagata attttgtcta taaatcagca ttggtgcgcg gtccaccata cactctaaat
    21961 aacggttcag ataccctgta ccgacatagt ttcctaatcg atagccttca acgccgaatc
    22021 ttgcgtattt ttgcgctaat tttgaacaat cagttacgtt cacagctaac tcttctactt
    22081 tgcggtaacc gcttaagata cgttcaaaag gttctaaagt gatttttgct tccatattct
    22141 tccccgtcca tcttccacat acttcatatc atttcacatg atacttatgc tctcatcata
    22201 ccacaggttg aattagaaaa gaaaataaat cacacaaaaa agcaagacaa tctgttagtg
    22261 agattgtctt gcttttcgaa aaagactgaa tacttttcgt taaaactggt tgcttagtct
    22321 tctaatgcca cattatggaa cacatgctgt acatcttcta gatcttccag tgcatcaatc
    22381 attttttcaa atgtttcttg atcttctgga gatagtgtta cctcgttttg agggatcatt
    22441 tggatctcag cgattgaaaa atcagtgatt cctttttctt ttaatgcttc ttgtatacta
    22501 tggaaatccg ctacttcacc gtaaacgatg atttgtcctt cttcttccat tacatcgcga
    22561 acatcgatgt ctttttccat taggtattca aggatctcgt cagcgtcttc tcctgcaaaa
    22621 gcaaagattg ctgtattgtc aaacatgtaa gaaaccgctc cggaaacacc catgttcccg
    22681 ccatttttcc caaatgctgc ccgtacgtct gctgctgttc gattcacatt gttcgtcaat
    22741 gtatcaacaa tcaccattga gccgcttggt ccaaatcctt catagcgcaa ttcagagtat
    22801 gtttcatctc ctgcaccttt tgctttttcg attgctcggt caataatatg ttttggtaca
    22861 ttataagttt tagcgcgatc aatgacgaaa cgtagttttt ggtttgcatg cggatcagga
    22921 tcacctgatt ttgctgccgc gtagatttca atcccaaatt ttgcataaat ccggctatta
    22981 ttagcatctt tagcagtttt ctttgctacg atatttgccc atttacgtcc catattttca
    23041 cttcgctttc tgatttgata gtttcataac gcaaaaacgt cctgttccat tatacttgtg
    23101 aatcacgatt ctgtgaagct ttttttcttt cttccttcat tcttctcatt tttagtcaat
    23161 tatctatact cggattggga tttgcccgaa attctgttcc ggaatattgc ctcccaattg
    23221 atagatttga tcacttctaa ctaacagttc cgcttcttgt gcctcttttt ttccgattcg
    23281 ggaataaata gaaatctctt ttttttcttt taagaatctt cttccagcat cggtaaatcc
    23341 aagtagtcga agataatttt tattttgttg gacaggaatc tcttgctttt tgatattcag
    23401 caagacataa caagataatc gttggatccg tgtccatgta tatctttttg tttttacctt
    23461 tgacacaaaa tcctcaaaac tatcagcagt tgcagctgct gttttcatgc gcgattctat
    23521 tccttcggtc atctgataga tatctgtcag ttccagcaaa gtagaagata acaaccgata
    23581 acgcaaatac ggccaataag attcccaagt ctgcagcgaa caagttgaaa gatcttcttc
    23641 tgtctgaggc ggcacaagtc cagctattct ttttccttct tctaacgcat ggcgaatggc
    23701 tgttgcactg gctatcctct gatctgtgat gattttttca tgatagcctg cacttttacg
    23761 tcggatcgga tgtaattcca tagggtggac atacttggca ttttcctttg cataacttaa
    23821 tcccaagata tgatttggcg aagaaaaatc aagcgtaata tcgggataca gactgcgaaa
    23881 aacctcagtc attttttgcg gataactata cgtttgatca gacagttctt gaaatgcctc
    23941 ttctatccta tcttgattct cttggacaaa gcgtccgaaa ctgctgtaat caaacgaagt
    24001 ctcttcttct gttccaaaac acaaactgtc acagtccagt gtttgcaaga tttttactgc
    24061 acctttagca aaatagtcag ctgactgaac cgcccattcg acgggaagct ccactactaa
    24121 atccacacca ttttgtaaag cttcattcgc gcgatgccat ttatcgatga tagccggttc
    24181 gcctctttgc aaaaaattcc cgctcattac agcaatgacg acctctgctc ctgttatttt
    24241 tcgtgccatt tccacatgat agcgatgtcc attgtgaaaa ggattgtact ctacgataat
    24301 cccgcagctt ttcatgcgca tcctccttca tagttatcga taaaaaagaa atagagacaa
    24361 gtaaaaaaag gcaaaaatcg gaaaaaatac ccgaactata taaactattc tcgcaaacac
    24421 aatacttctg cgacgaaaag ctgcactgca agagcaatgc ctatatcgtt cggatatata
    24481 atttatgttg aagatttatt cttttcttgt taactcgagc aggcaaaacc gctagtcttt
    24541 gtatgctaca aagaaccagc gctttgattt ctctgatgct gcttcgtctg taaaatcaga
    24601 aaatgcttcg actttcgaaa agccgctgtt ttccagcatt cttaaataac tttccaatgg
    24661 ataggtacgc tcttgatgta gttcatcttc acgaataaat cgaccagacc cttcttgttc
    24721 ttcaacgaaa aaggtaagaa aatgttcgat gctgtgtttc tcgcttcctg gataactatc
    24781 ccacaaaaaa gcaaaattat ccccttgata atgatagcta tattctggaa aaacctcatc
    24841 tacttgatag gtggagtgta cgtcaaataa aaatcggcca ttttcttcta acgcttgata
    24901 gacttcatca aacacttgct gtacctcttg tctatttttc atatagcaca gcgaatcaga
    24961 aaaacaggta attgcctgat attgtccgat ttctgataaa tcgagcatat tgccttccac
    25021 aaattggatc gatgcgtctt cttcatatgc tcgctggctg gcaatcatca acatctcttc
    25081 tgaaaggtct aaccctgtca ctttatatcc atcttttgca aaggcaaccg ctaatgctcc
    25141 agtaccgcat gccagttcca aaatattttt tgtgtcttct tgtaaatgac gtttcgaaaa
    25201 agctaaccat tgctgataaa gtgtttcgtc catcacttca tcgtaaatga aagcaaatgt
    25261 ttcataagcc atatgttaat ccaaccattc actaagatcg actagcggag catctgacca
    25321 tagtttttcc agattataga aatttcgttc tggcgcatgg aaaacatgta cgatcacatc
    25381 tccaagatcg atcaataccc attttccgcc ttcttttcct tcaatacgtt ttacttcata
    25441 tttgttttct tcttctttat caatgatttc ttctgtaatt gcgttgattt gacgttcgct
    25501 atttgctgaa cagatcatga aataatctgc caacaaggaa acatttcgaa cgtctaaagc
    25561 gataatgtct tctgcccgtt ttgaatctgc tgcttcaatc gcaattttta attgttcttt
    25621 actatctatg atgatttcct cctaatatga gcagcttatt tttttgctac ccagtaatta
    25681 tatgtttcga ttgtttttgg ataaatctgt tgttcttgcg caagcaaatg ggccaatgta
    25741 tgttttgttt caaatgctac agcttcgtct agatcagccc atgcaatggc tcgtgcatct
    25801 tgtaccccag gaaaatctcg acctggttca atatagtctg ctacataaat gattttgtca
    25861 aggagactca tcttagcagc cccagtcgta tgcacccgga ttgcatgaag aatctctgca
    25921 tcggtgattc ctaattcccg ctctacaaac gaagcgccaa caagaccatg ccagattgca
    25981 ttattataat tcaataactc cggatcaaaa ccgtctctta cgattaccat tttgaactcc
    26041 tcatctggtc gttcttttgc ataatcatgt gtcaatgcag caatactagc tttttcagga
    26101 gaagcaccgt attttttggc aagagccacg gctgtttctt ctactccaag cacatgcttg
    26161 aagcgttgtt cgctcattct catctgtatt ttttgcatca ggttttctct agaataagca
    26221 gtatattcat tactatagtc catattcata caacccttca ttttgaatat aatcaattac
    26281 tttatctgga acaaggtagc ggatagaaca gccttctttg atcttttgac gtattttcgt
    26341 ggaactgatg tcgatctcag gtacatctac ccaaattaca ggatatggag tatccgttgt
    26401 gtatcctggt cgacgtatac ccacaaagtt gaccatagaa gtcagctcat cgattttata
    26461 ccattttggc aaatattcta ccatatcccc accgataata aagtaataat ctgtatccgg
    26521 attgttttgc gtcaattcct tcatagtatc ataggtgtaa cttttcccac cacgagctaa
    26581 ttcaatcgtt tcaatctgca aaaaaggatt gtcttccacc gctaattcca acatgttgag
    26641 tcgatgtttt gcatcaatcg tctgtttctc atcaacatga ggtggctgat aagaaggcat
    26701 caaaaatacg cggtcaagac ctaaattccg gccagcttgt tcagccatca ctaaatgtgc
    26761 aaggtgaacg ggattgaatg ttccgcccaa gatccctact tgctttttct tctctaagaa
    26821 ttgaggcatt tcttcaggaa ataattgtga acgaacaaat gtcttagctt gcgtattcat
    26881 catggtcact ccttatattg cttttacttc ttttgatagc ctttgatact tttcttttgt
    26941 ggaaggtttg tataatacaa taacacgtcc aatgatttgg acgatttcgc aatctatttc
    27001 tttttctaat acttgagctg cttcttctgc aatctcatct gtattttgaa gtaacgatac
    27061 tttgatcagt tctctttttt ctaaagcttc ttcaatttga acgatcattg ctgaattgat
    27121 cccacctttt ccgatttgaa aaatcggctg aagatgatgc gctttgctac gtaaaaaacg
    27181 cttttgtttt cctcttaaac tcataatttt ccttcttcct taaatcaatg ctttgcgttt
    27241 caatacatct acaccctttg gcgcccagcc cgcaattact actggcgctg ttacagtgat
    27301 ccagcctaga ccggcaaaaa cgatgtctgt tttttctttc acagaaaact caaaacgaac
    27361 aagctccgga aatgcctcga cttcatcagg acgaggaggc tgtaataatc cacctacatg
    27421 tttttgataa aattcgtctg ctttttccag tttcgttcga tgaatattta catcatttga
    27481 cacataagca ataaatgaag cacgattgcc gctgataaaa tcaaatcttg caagtccgcc
    27541 caagaaaagt gtttgccctt cattcagctg ataaacttta ggtttgattt cttttaacgg
    27601 tgcaatgatc cgcaaatctt ttttacctaa gtagtgcgcc atttgatggc gatggataat
    27661 tcccggtgta tcaatcaaaa aatgcccgtc atctaaagga atctcgattt tatccaaggt
    27721 cgttcctgga aactgcgaag ttgtgattac atcttgtact cccgctgtat ttttgatgat
    27781 ttgattgata agcgtcgatt ttccgacatt tgttactccg acgatataga catcccgtcc
    27841 atcgcggtat tcttcgattt tttctaatag ttccgtcatc tcatgagatt tcttagcact
    27901 agttaataat acatctactg gacgaattcc tgcttcgtaa gcacgttcct tcatccattg
    27961 tgttaattta gtttttttca atgatttagg taatatatcc actttgttcc caactaaaag
    28021 tactggattg tcaccgacaa aacggtgtag tcctgggatt agtgaaccat tgaagtcaaa
    28081 aatatccacc acattgacga tcaatgcatc tttttgccct aattcattca gtaagcgcaa
    28141 aaagtcgtca tccgtcaatg agacatcttg ggtctcatta taatggcgta atctgaaaca
    28201 acgctgacaa tagacttccc cggaagctaa acctttttcc aaagccgttt gcggcgtgta
    28261 tcctaattca ccgggtttgt ttgtttgtat aacggcacca cagccgatac attgtaattc
    28321 ttctgtcatt cgattccacc tcgccagccc atatcagggt gttttgctaa tagataggcc
    28381 ataattcttt tttcccaaaa acgattaaat tgtgtcttcc aactatctgt tgcgataatg
    28441 ggttttacaa ggatgctacg tatccctgca cgattggctc cacgtatatc agtcataatc
    28501 tgatcgccta ccattactat ctcatcatct gctaaatcca attgtttctt tgctatgttg
    28561 atccctcttg ctaaaggttt cattgctcgt gcaacataca aaagatcaaa tttttcaatc
    28621 gctcgcgcga cacgttttga attattgttt gaaacaacaa caacagggat tcctgcattt
    28681 ttcatttcta aaatccaagt aagcaactct tctgttccat ctggattatt ccatgcaatc
    28741 aacgtattgt ctaaatcggt caatactgct ttgatgccta gattctttaa ttgggcaggt
    28801 gtaatctgat agatggcttc taccatccat gttggtttgt aatttgaaaa catgctgtct
    28861 cctcatctgc aaaaaatgaa ggagcacata aagtactcct caaccccgta ttatactgca
    28921 tatcagcaaa aaaaactacc tctatactcg ttcgtctttc agaaaaagca tttctttaca
    28981 aaatgaccaa aaataaagat tgatcacaga aaaatttaga aacaaaaaac aactaatatt
    29041 ggcggaacct cttgtatcgt ttttccagta atttatgctg atctaatcga acaagttccg
    29101 tcaatctttc aatcaacgct tttttcataa tgtgtgcgac tttatatcgt ggcaactgct
    29161 cgccgttgaa cgtttcagga ataactcgat caacgattcc taactcgcgt agttctggcg
    29221 cagtaatttt catcaactca gctgcttctg cggcgcgact accgtctttc cacaaaatcg
    29281 acgcaaatcc ttctggagaa agcacagcat aaatcgtttc ttccagcatc cagacttcat
    29341 ccgctaaacc tagagcaagg gcaccgccac ttccaccttc accaatgatg atggcgatga
    29401 tcggtacctt taaatcagac atttcgagca gatttctggc aatagcctct ccttcgccgc
    29461 gctcttctgc tcctacaccg caatatgcac ctgctgtatt gatcaaggta atcaccggtc
    29521 tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct tcaggatggg
    29581 gagaaccaaa atttctgcgg atattttcct gcagatcccg tcctttttga ataccgatca
    29641 ctgttaccgg tttgccatct aatcgagcga ttccaccaac aatcgcttcg tcatctgcaa
    29701 ataaacgatc accatggcat tcctcgaaag aatcgaagat ctcttcaaaa aaatccaagc
    29761 ttgtccatcg ttctttagct cttgctaact gaacgacatc atgtgctgtc ttatccacga
    29821 tctatccatc ctttctgtgt atgcaattca agaagcgtat ggattttctg tttcaattcc
    29881 atgcggggaa caatctgatc gacaaaacca tgagataata agaattcagc cttttggaaa
    29941 tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc aaaaccgatc
    30001 aagctttggg gttctgctag aataatatcg ccttccattg caaaactggc agttactcct
    30061 ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac cttcattgct atgccgtttg
    30121 acagcagcag agatttttgc catctgcatt agagaaaaga ttccttcttg catccgtgcg
    30181 ccacctgaag ctgtgaacaa gacgactgga agactttcct ttgttgctcg ttcgaataat
    30241 ctggtgatct tttctccgac aatcgttccc atacttccca tgataaaatt aggatccatc
    30301 actccaataa tgaagggaat cccctgaatg gttgctttac cagtgaggac tgcttcatga
    30361 agtcctgttt tatcttgcat ttttttaatt ttttccgtat attcgggaaa tgctagcgga
    30421 tctttggtta caagatccgt atcccattct tcaaaacttt tttcatcaat aatcaaagct
    30481 agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat gagggcatac tttttcttca
    30541 ccgatgtctt tcgtataaag gatatgtttg caatttggac actttgccca catgttatca
    30601 ggaacagatg gcgagttagc aggtttgctc tctctattag gattgatccg aatatatttc
    30661 tttttcttaa acagagccat ctatgcgcct cctatctccg tttctggtgt ccaattcggt
    30721 aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc aggatgacta
    30781 atcaaatcca tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa ttcacttaag
    30841 gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc catggacgat tatttttgca
    30901 atcattgaat cataatatgg cgggatcgta tatcccgggt aaactgcact gtctactcgc
    30961 agtcccatcc cacctgctgg aagcagtaag ttttggatct tccctggaga aggagcaaaa
    31021 tggaaagcag gattttctgc attaatccgg cattcgattg cgtgtccttg gaatactact
    31081 tcttcttgac acacattcaa aggctcaccg gctgctatct ccacctgttt ttttactaaa
    31141 tcgattcctg tgaccatttc tgttactgga tgttccactt ggattcgagt attcatttcc
    31201 atgaaataaa aatcgccttc ttcatccatc agaaactcga tcgttccggc attttcataa
    31261 ttgactgctt tagctgctcg aacagcagcg tctcctagtg cttctctttt tgtttgtgag
    31321 atcactacag aaggagattc ttccagtact ttttgattgt tgcgttgtaa agaacaatct
    31381 cgctcgccta aatgaatgac atgaccaaat cgatcaccta agatctgaac ttctatatgg
    31441 cgagcaggat aaatgatttt ttccaaatac atatcatcat ttccaaatgc cgctgctgct
    31501 tcttgctgag ctgaagaaaa atgctgtggg agctcttctt ttgattgaac tttccgaatg
    31561 cctttcccgc caccacctgc agctgctttc agcatcactg gataaccaat tctgtctgct
    31621 atttccaatg cttcttctac tgtagataat acaccagtac ttcctggaat gactgggaca
    31681 tttgcttctt gcatcaattg acgcgcattg atcttatttc ccattgcatc gatcgttgca
    31741 cttttaggac caataaatgt gatattgcac tcttcacaca tggaggcaaa tcggctattt
    31801 tcagataaaa aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat tgcagcgctc
    31861 agtacttcct gaacattcag atatgaatca acggcttttg ccgggccaat gcagattgct
    31921 tcatctgcta actgtgtatg aagtgcttcg gcatctgctt ctgaatatat cgctacggtt
    31981 tggatcccta gttcgcggca tgcacgaatg atacgtacag cgatttcacc gcggttagct
    32041 atcaatactt tagaaaacat atttcaccta tccaatcata aaggttaatt ccgcttcggc
    32101 tactttttta ccgtctactt tagcgattcc tttgcctatt ccggctgaag ctttcacttt
    32161 caatatctca acttcaaggt ataaggtatc tcccggtgta actttcttac ggaattttgc
    32221 tttgtccaat ccgccgaaat aagccgtctt tcccttgaat tgttcaagag aaagtaatgc
    32281 aactgctcct gcttgagcca ttgcttcaac aatcaatacc cctggcataa caggttcttc
    32341 tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca tttttcttag caacgatccg
    32401 ttctccttca accatttctt ccactcgatc aatcaaaagc attggataac gatgtggaat
    32461 gatttctttt atttcttgta tgttcattgt tttccctctt tcctgctttg cttatttttg
    32521 ctgaatccta aagagtggct gtccatattc gacaactgac tcgttttcta caaggatttc
    32581 tgtgatcact ccgtcggcct cactagttat ctcattcatc agtttcatcg cttcaataat
    32641 gcagataact tctcctttgg caacttgatc acctactttt ttgaaagctg gtttgtcggg
    32701 cgctggttgc agataaacga ttcccacgat tggcgaaaca acctcgattc catcaaatac
    32761 tgccgattct ttttctttca ttatttctgg agcagttgtt gcttgttcag gtatgacatt
    32821 gactggctcc gaaactggca acgtttcgtt tgtttgaacc atattcatgc ctcgacttgt
    32881 tttattttta ttcatataca attcaaattg accttcacgc aaatcgaatt ccgttagcga
    32941 ggattgatca aattgactga ctaattctct gatttcattg atatccaaaa ttattcctcc
    33001 cagcgtttca tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt actcattgca
    33061 taagtaaaac ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat ctcaggatct
    33121 tgtttttcta cattgatcgt aggtgggata aattgatgtt ctaatgccag caaagttgcc
    33181 aaggcttcga tcccgccggc tgctcccaat aaatgtccag tcatcgattt cgtgctgctg
    33241 acgtaagtat ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc ttctgcctgg
    33301 tcattggctg gtgtacttgt tccgtgagca ttgatatatc cgacttgttc cggctgtaca
    33361 ccagcttctt ccatcgcaag cttcattgct ttagatgcac cagaaccatc tggagtagga
    33421 gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa cgatttctcc atagattttg
    33481 gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta gaacgcctgc tccttcaccc
    33541 atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac cacgattagg atcagttgat
    33601 tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat cgttgcttct
    33661 gttcctccag caagaatgac atcagaatag ccatgtttga tgttacggaa tgcttcccca
    33721 attgaattcg ttccgctagc acaagctgtt acgatagctg tacaaatacc ttttgcacct
    33781 acacgcaaag caatatttcc agctgccatg ttggcaatcg ccattggtac gaacattgga
    33841 gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt ggttttcgat tgttggaaga
    33901 ccaccgatcc cagatccgat gatgacgcca aaccggtctt gattcatttt ttctttatct
    33961 aaaccgctca tctccattgc ttctaaagca gcgtagattc catagatcga aaacatatcc
    34021 attcgtttgc tatctttttt aataaaatat ttatcgtaag ggaaatcttt gacttcaccc
    34081 gctaaggtaa tccctgtttc tgaagcatca aatttagtga tcggtgcgat gccattcgtc
    34141 ccctctttta agctattcaa aaagctgtct gcgtcatttc caataggtga agtgactcca
    34201 tagccagtaa ttaccactcg attcatttgt ttgctctcct tttcttatcc gtgcatgacc
    34261 aatccgccat cgacattgat gacttgtccg gtgatatatg gattttgtgc taagaaaact
    34321 gctgtttgtg caatatcttc tacttgtccg aaacgctgca tcggaatttg cttttccgct
    34381 tgttccttga ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa tcctggcgca
    34441 atggcattac atgtaatgcc tctagttgca gcttctcgtg ccacagattt tgttaatcct
    34501 actacgccag ctttactagc tgcataattt gcttgaccaa tatttccaat caacccagat
    34561 acactagaga gattaatgat ggccccttct cgctgcttca tcattttttt caatacgtgc
    34621 tgcgtcatat tgaaggttcc aatcaaattg atatccagac attttttgaa atcttctgca
    34681 tccattcgca tcacaagttt gtcatttgtg attccggcat tgtttactaa cacatggatc
    34741 gatccaagct tttcttctgc ttctttgatc atttgtcccg ctttctcata atcagagatg
    34801 tcaccggaaa ctcctacgca ttttacaccg aatgcttcga tttcttctat cttttctttt
    34861 ggaatctctc cgcgaccatt caaaatgata tttgctccag cttttgcaaa agctaaggcc
    34921 atcgctttgc cgattcctct tgtactacct gtgataaata cattttttcc ttttacatcc
    34981 ataacgttcc tccttatagt attgctattg tttcatcaaa tgtctgctta ttctcaacac
    35041 gcagaactgg tattgtttta tcgatttttt tcataaatcc gcttaatact tttccggggc
    35101 cgacttcgat gacttgttcg atgccgattg tttttaactt atggatactc tcatagaaac
    35161 gaacaggttg catgacttgc tgttcaagta gtcccgcgat tgtttctttc ttcattattt
    35221 ccgttgttgt gttgcttatg ataggaaaag aaggttcaga gaattgaatc tgacttaggt
    35281 ccttcgccag tttttttgct gccggttcta aaatagctgt atgaaaaggc ccgctgacat
    35341 ttagagggat cattcttttg aacccttttt cttttaatag taaaacagct tcgtctactg
    35401 ctttttcttc gccaccaatc acgatctgct gaggtgtatt ataatttgca ggagatacga
    35461 tgccgtactt gctagcttca tgacagcttt cctcgatcgt ttcaatcggc gcattcatca
    35521 ctgcaaccat tttcccgctt ccagctgggg cagcttctgt catataagcg cctctttttg
    35581 ctactaacgc aactgcttca gtgaaggata gcgcattact agctactaat gccgaatatt
    35641 ctcctaaact cagtcctaaa gctgcatctg ggatgattcc atgctccttc aataagcgat
    35701 aatacgcgat gctgactgtt agaatagctg gctgtgtata ttgcgtctga tccaaacgtt
    35761 cattttctgt aaagcaaagt tcagccatat cgtagccgag aatctcactt gcttcatcaa
    35821 atgtctgctt tacaacggct tcttcatata aatcttttcc cataccttga tattgtgcac
    35881 cttggccact aaataagaat gctgttttca tgtttttctc caatatatcc agaatatatg
    35941 tttgattctt cctattcaaa aaggccgaga cattcctatt tcaggaaatg tcccagcccc
    36001 caacctaatt ttaatgatta tgcagtttgt ttttctacgt aagtcactaa atcttgaacc
    36061 gttttgattc cgtcttctgt ttcgattttc acgtcaaatt catcttcaat ttcattgatg
    36121 atttggaata aatccaagct gtcagcttct aagtcttctt ggatgtcagt agtcaatttg
    36181 atttcttcag tttcttttcc taattcttct gcgatgattt cttgaatttt ttcgaatacc
    36241 ataataatat attcctccga tattgtttgc tttttatttt tataatttaa cagtctacaa
    36301 ctccatgaga atacttcccc atgttaaacc tccgccatag cctgtaaata ttactttctg
    36361 ctgttttcct aaggtgatct ttccactaga aactgcttca tccaataaga tagggatgct
    36421 tgcagcagat gtattcccat atttatccat attggtcagg aacttttctt gcggcatctt
    36481 tacttttcgg gcaattttct caataattct gaggtttgcc tgatgcaaaa gcagataatc
    36541 aactggtttg tctttcatga cttcttggat attgtttgca acgtctcgta cggcaaaatc
    36601 aaaaatatct ctgccagtca tttgcaaata atacgaacct tctgaatctt ctgaatagaa
    36661 cggattttta ttttgctggt agccagcggt caatgagcta cttctggtac cgtcagctgc
    36721 taatttttct ttcagcaatt ttttctgtgg attcgcttct aagagaatcc cacctgctcc
    36781 atcgccaaac aacacagcag tcgtacgatc agaccaatcg attagcttag atagtgtttc
    36841 accaccaatc accagtccct tagttgagcc gcagcggatc attttttcag ccatgcttaa
    36901 cgcataaaca aatcctgagc acgcagcact gatatcaaaa gcgatagcat gagttgctcc
    36961 caatttccct tgtacttgtg ctgctacaga tggtgtcgtg taatcgggcg acattgttgc
    37021 tactaaaata aaatccaaat ctttagggtt tagatttttc tgttttatca atttttccgc
    37081 cactaaaatg cataaatcag aagtctcctg atttgttaca catcttcgtt cacgaatacc
    37141 cgtccttgac gaaatccatt catcagaggt atccatcatc ttggaaagat cattattagt
    37201 aataatcttt tcaggaagga cgctggctgt tgcagtaatc attccaaatt gttccattaa
    37261 atatcctcac ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat ccagcaaggc
    37321 atgctgttcc tccgtagcca ttccgtctaa gatgttcttg accatttcac gatgaaaatg
    37381 ctgatggacc cgaaatagta actttccttt tttcgttagt cccaacttta caacgcggcg
    37441 gtcatcttca ctgcggatac gttcgacata gccttttttt actaacttgt taatagcagt
    37501 ggtcaaggtc ccaacagtaa ttgatagttc tttggctact tctgaagttg tttttttctt
    37561 gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt gacagatctt tgaattgaga
    37621 ttttttcagc tcagactctt cgattgtcaa aatatcattg aagacactaa caagataatc
    37681 atttactgtt tttaaatctg gttccatgtt ccaccgtcca ctaattagtt tgattatcaa
    37741 atcttttgat gttcaaacta tatcttaact tttttcaaaa tgcaacctta aaagatcatt
    37801 tttttactat atgagctttt tttaagattt tactcattta aaacaactga taaatcagca
    37861 tttataattt cttttattag aaaaatcatc tatctcacat attttgatta tcaaagtatt
    37921 ttctgtgaac agaactatag aattccctaa aacttgtcca ttatttcaaa atttgttata
    37981 gtctttttat tcaaagcgta aaaggagatc ctatgttaaa agaaaaaatg aaaactctcc
    38041 ccatgctgcc gggtgtctat cttatgaaag accgagatgg taaggtcatc tatgttggta
    38101 aagcaaaacg gctcaaaaat agagtaagca gctattttca tcagaataaa cagcattcaa
    38161 aaaaagtatt acgtatgatt catcatataa cagattttga tttcgtcgta gtagacacgg
    38221 aattagatgc tttattgctc gagtgtcaat tgatccaaca ttaccgtcct ttttataacc
    38281 gacaaatgaa ttatttcagt aattataatt atgtacatat taccaataag ggatttgtat
    38341 taacagatac tcctacggct cgaacatacg ggccttttcg tctctataaa aaaatgccgt
    38401 ctattttgcg tataatggaa gaaacttatc aaatgccttg gttgtctgaa attagtttgt
    38461 tagctttgcg agtccagctt cctgacctac aggaaatgac ttttgaacag aaaaaaaagg
    38521 aactccaagg gctgtttcaa ggccgaaata aaaagctact gacttacttg aaaaaaagac
    38581 aacaacattt catctatcag ttgaatttcg aaaaagccgg tatgcttcaa aaagatatcg
    38641 aattagttac ttattttatt agacggatcc aggaacaaaa gcagttttta agaacaccat
    38701 cgctcacctt cagcatgcca ttagcagcag atgagagtca gaaaaagcac tacctcgttt
    38761 gttatggtca acttgcagaa acaatgatta cctctggtga cgtttctcct gacttctatt
    38821 atgaaaagaa agaagctcat ttatctttga aacgtcaatt gtcaaaagaa gaaatcgatc
    38881 ctgtacaaat cttgatcagt taccgcaaaa aattagaaaa agaacagttt gaaattgaac
    38941 tgttaaacaa aaaagaagct gaaaagcaac taaattagtt gcttctcggc ttcttttagg
    39001 catcttcaac ttcaaatgtc aattatccta aaatttattg gattaagtca tagatttcca
    39061 tcgctacgat atcgatatta tcaaattcgt attgctgatg agtatctgtt tcagttaatt
    39121 caaatgtatc ggtcgcttta tcataagtta ctgtacactt ctctacgcct tctttttcaa
    39181 aacggcgaac ttgcagttca ttatctgctg actcgaccat agcctccagt cgttttatga
    39241 ttgcgactaa ttgtgaatgt gtcatcggag gtcctccttt tcttatttct gttatattgt
    39301 atcaaaagca tagcctactt gcattgattt tctctaaaaa aactcgtttt ttatgatttt
    39361 ttttctggta tttttccttc aggccaccaa agtttgatca aagcttgggt tccgtcattc
    39421 tcaaaacctt tatctgccaa ctcttcatat agtttcaatg cttgggtagt tgctggcaat
    39481 acaagatcca ttttttctgc ttcttctaat gcaatcttta aatctttgat aaaatgtttg
    39541 acaaaaaaac caggtgtgta atcttctttt aaaatacgag gagaataatt gcttaaagac
    39601 cagttagctg cacttccacc tgaaagcgtc tcaatcactt tctttaaatc aagtccgttt
    39661 ttttgtgcat agacaagcat ttccgtcatt cctgtcatcg tacctgcaat catgatctga
    39721 ttagccattt ttgtatgctg acctttccca gcagaaccat gcaatgtaaa tgtcgtacca
    39781 aattctttga ataaaggcag tacttgatca taaacggctt cttctccacc gaccatgatc
    39841 gtcagtgtac cattctttgc tcctaagtca ccaccggata ccggtgcatc taacgcatct
    39901 gcgccttttt cttttgctgt ttttgcaatt ttttctgcca ggcttggtgt acttgtagtc
    39961 aaatcaacta aaatatgtcc tgagatatct gcagaaaaaa tccccgactg tccatagtag
    40021 atttcttcta catctttggg gtaacctacc attgtaaaga gaatattact ttgttctgct
    40081 accgcttttg gtgtatctgc ccagatagct ccttctgtta ctaaaggatc agcttttgct
    40141 tttgtccgat tatagacagc tacttcgtgt cctgcttcta gtagatggcg tgcgacagca
    40201 cttcccatga cgcctgttcc gataaaacca agtttcattt tttcgacctc ctaaaaatca
    40261 aaaatccttg cttctatgct agtatacgct agaaacaagg attttttcat aaaaaatgtt
    40321 tgaattttat cttttaaatg aatttaattt tattttgatt cgttcaatcg agtcttcttc
    40381 ttcttttctt cgcatctctt cttcatgata aacaaccatg tcttgataaa attcgatcat
    40441 agcttcgccg aaatgtgtag atgaaatatc ataaagttta gcttctaaaa cttttggatc
    40501 tttaggaact tcactggaaa tataatcaat aagtgctggt gcaaagtctt cgtcggtttc
    40561 aaaagttttc cctaatgaag gatgatcgaa caatcggttc aaataagcat ttccttccac
    40621 gacacaaggt gttccggctg ccatcgcttc tgtataagta agcccttgtg tttctgaagt
    40681 agaagcactg acaaaataat ctgccgcttt ataataaagt gctacatcat tgttgtccac
    40741 ttctcctgtg aactgcacaa attcttctaa ctgcagttct gagatcaatt cctctaactc
    40801 ttctacataa ggtccttttc ctacgataac taatctggcg ttaggataag cggagatgac
    40861 ctgaggaaac ccattaatca aggcttggat atttttctca taagaaatcc tgcttaaaga
    40921 aagaatcatc aaatggtttt cctgaagacc tagtgactcc cttaatttgc tgatatcctg
    40981 ctgagtgata tcagaacgtt tgaatttttc aatgtcgata ccagtaggaa tcacgcgaag
    41041 cggtgctatt acaccatagc tgcgtaatgt atcaataaca cgttcacttg gacaaactac
    41101 acctgtcgta tgatttgcaa ataatctaga caagtatttt acatgggatt gcctgatcac
    41161 ttttccttta ccgatataat gaaggtagtc ttcatacatc gtatggtaag tatggatcag
    41221 aggtattttc aacttcttag ctaccatctt cccaagtatc cctgctccga attctgtatg
    41281 ggtatgaata agatctagtt ctagttcttt ggcaaccaaa taagcatacc acatgcctct
    41341 aacaacaact cttcgatcct tgaaagaaat aaatggtaca cttggcattc gaatcacatc
    41401 tttttccaac ttatccgcat taggatcggt cgttgtaaaa atatagactt catgcccatg
    41461 tctttctaat tcttgtttta atgttttgat cgatgtagca actccgctga cttgcggaaa
    41521 gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc tccaaactag tccttgacct
    41581 gatttacttt ttttaatttg ctagagactt tttcattcag tacactttcg tatacatttt
    41641 taagttcttc accgatttta ttgatacttc gctcttttgc tgtttgataa ccggcttgtg
    41701 tcgtactcgg tagttcttga ttggtaatct gttggatcag tcggataaat tcttcgtttg
    41761 tcttccccat atagcagttc ttgctatctt ctaaccagcc attatacact ggaatatctc
    41821 gaacaagtac gttttgtttg cttgctaaag cttctaaaac aacgatccct tctgtctctt
    41881 cataagaagg gaaaaagaat agatctgcac ctgaatacgc accttctatg atctctccac
    41941 gaatgtaacc aggaaacagg acattttcag gatgatcttc ttttacaatt ttccggatgt
    42001 tccgaggaat cgagtacatg ggaacatgac caaaccaaat aaatgtgtat tcgggcatct
    42061 tcttagcaat ttctacgaaa tcaatgattc ctttacgttc gaaaaacaaa ccgacacaaa
    42121 tgattacttt ttgatccgga gacagtttga aatattctct gaacttttgt tctttcattg
    42181 gatcagggtg atatttctct aagtcgatcc cattggaaat agcttgaatc ggcacacgga
    42241 taccatagct ttccaacaac tgttttgaat aaggtgttgg cgtgatcaga tagtccgctt
    42301 tttggtacaa tcctaccaga tattttttta cgataggaga aagctggttc gatccgataa
    42361 aggaatttcg gaaatcttcc tctgtagaat gcgcatgata gacaactttt tttccttctc
    42421 ttctggcttt attgaccata ttatgactat tgattccgta tgtattgatg tgcaggatgt
    42481 cataatcttc tttttcatct agtgtataag agataccgac ttccgtcaga gcacgttttt
    42541 gatgatcaag cgcacgcccg attccagatt ttgcaagcat cttttcactt tcaaaatata
    42601 ataatacctt caccttacgt cccccttcac ttatatagta taacatagcc tacaaactgt
    42661 cactcaaact taagccctat ttttgtctat tcttactgac aaaaacgttt tcttaaaaaa
    42721 gaacaaaaaa actggcgtag ctcttttaca agccacacca gcttttgata cgttgtttat
    42781 tttacatatt catgaacaag ttcaacgact tcttccatcg tatcgcattc tttcaatgca
    42841 cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt tcattaaact acgcgtttta
    42901 agaatagatg tcgcactcat tgagaactca tctagaccca tacctactag caatggaaca
    42961 gctgtttgat ctccagccat ttctccacac atacctgccc attttccttc agcgtgtgct
    43021 gcatcaatta catttttgat caaacgtaaa attgatgggt tatatggttg gtacaagtaa
    43081 gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt gaatcaaatc gttcgtacct
    43141 acagagaaga aatctacttc ttttgcaaac ttgtctgcta agactgctgc tgctggaatc
    43201 tcgatcatga tgccgacttg gatcgtatca gatacttcct tgccttcact tatcaatttt
    43261 tgtttttctt cttcaaagat cgcttttgct gcgcggaatt ctttgagtgt agctaccata
    43321 gggaacatga tacgtaaatt accatgaaca gatgcacgaa gcaatgcacg catttgtgta
    43381 cggaacattt cgtcgccttg ttcagataaa ctgatacgca atgcacgata tcctaagaat
    43441 ggattcattt catgaggtaa ttgcagatat ggtagttctt tatctccacc gatgtccatt
    43501 gtacgaacaa ctacaggttt tccttccata ccttctaata cagctttgta agcttcatat
    43561 tggtcatctt ctgttgggaa gtctggagaa tccatataca ggaattcagt acggtacaga
    43621 ccaactgctt ctccaccatt attatggaca ccaactagat cttttggtgt tccgatgttt
    43681 gcagctaatt caaaatgctt gccgtcagct gtgacagttt ctgcattttt taatttttcc
    43741 cattctgctt tttgtgctgc gtaatctgca cctgcttttt caaattcagc tttttgttca
    43801 tctgtaggat cgatgattac atcgccttcg atcccgttga cagccaaaat atcgccttct
    43861 ttgactttag cagtaatttc ttttgtccca acgattgctg gaatttctaa agaacgtgcc
    43921 atgatagcag aatgtgaagt acgtccacca atgtctgtta caaacgcttt tacaaaattg
    43981 cgatctaatt gtgctgtatc acttggagtc aagtcatgtg caacaacgac cacttcttcg
    44041 ttgatcattg atggatttgg taaagtaacg cctaataagt gcgctaaaat acgtttagca
    44101 acgtcacgaa tatctgcagc acgctcttgc atataagcat tatcttccat tgcttcgaac
    44161 ataccgatat acatatccgt tacttctttt agcgctgatt cagcatttac gctgttgtct
    44221 ttaatatttt gcttgatttg acctaccatt tctggatctg aaagaaccat caaatgggca
    44281 tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg ctttttcacg aatttgctgc
    44341 aattcttcag tagatttagc taatgcagca tccaaacgag cttcttcagc cgcagtatct
    44401 tctactgttg ccttactgaa tgttaaatcc ggttgaacta gcaggtaagc ttttgcaacg
    44461 gctacaccgt cactagcggc aatccctttt agcatttcaa ccattattca gataagcctt
    44521 ctttcttcat tgtttcaacg attgctgcca ttgcatcagc ttcgtcagca ccttctgcag
    44581 taatagtaac atcagaacct tgacctacac ctaaagacat aacgcccatg attgatttca
    44641 agtttactga tttaccttta tactctaagt ttacatcaga gttaaatttg cttgctgttt
    44701 gtactaatag tgtagctgga cgagcgtgga tcccagtttc tgctactacg tgaaattctt
    44761 tcttttccat attaatcggt ctcctttaag tgagtgttgt ttttatgtga gggttttctt
    44821 ttttagtaat tcagagaatc ctaacaaatg ccaccctttc atctgtaaag attaacattt
    44881 tttttcgtta gatacaacag ttttcattca atttcagata aaagtattca aattattccg
    44941 gattcttttt catccttttc ttcacatttt ataacaaacg tgtttatttc ctttttcttg
    45001 tcgctttctc ttctcctgac gtttatcatt ctttcctttg accctctttg ttttcatatg
    45061 aaacgaatta cttgcttccc tttttatttt tagtataata agagtcaaag gtcaggaatg
    45121 gtcaatagag atttcacctt ttctacctca taaatctctt aaaaaactag gggttattct
    45181 tttacctcat gaaaaacgag atgtaaaata tggatgtacc catttttgtg aaaggacgtg
    45241 aatgattcat gctttgtcaa aactgcggca agaatgaggc aacgattcac ttgtatgcca
    45301 ctgtcaatgg tcagcggaca caactcgatt actgtcagag ctgttatcaa aaaatcaaaa
    45361 atcaacaaaa tggaggtctg actaatatgg cccaaaatga tccatttgga tttggcagtt
    45421 tggacgatct tttccgttcc atgtctcgcc aaatgcaaca gcaaggaaat tatgagcaaa
    45481 caccaccaac acaatttggc ggaaacaaca acttcaatgg tggtcagcca cctcaaggca
    45541 atacagaagg tttattggga gaatacggaa tcaatatcac agaacaagca cgacaaggtg
    45601 atatcgatcc tgtgatcgga cgcgatgacg aaatcaaacg agtcattgaa attttaaatc
    45661 gccgaaccaa gaacaatcct gtcttgattg gagaaccagg tgttggtaaa acagcagttg
    45721 ttgaaggttt agcacaaaaa atcgtagatg gtgatgtacc gcaaaaatta atggacaaag
    45781 aagtcattcg tttagatgtc gtatctttag tccaaggtac tggtatacga ggacaatttg
    45841 aagaacgtat gcaaaaattg attgaagaga tccgtcaagc agaaaatgtg atcctattca
    45901 tagatgaagt tcacgaaata gtcggtgctg gttctgctgg tgatggaaat atggatgctg
    45961 gtaatatttt aaaaccagct ttagctcgag gagaattgca aatggttggt gctactacct
    46021 taaatgagta ccgcatcatc gaaaaagacg ctgctttaga acgtcgtatg caacctgtac
    46081 gggtagatga accaactgtc gaagaaacga tcagtatttt gaaaggtttg caaaaacgtt
    46141 atgaagacta tcatcatgta aaatatactg atgaggccat cgaagcagcc gcaactttat
    46201 ccaatcgtta cgtccaagat cgcttcttgc cggataaagc catcgatttg ctagatgaaa
    46261 caggttctaa gaaaaacttg acgatccaaa tcgttgatcc taaaacaatc gagaaaaaac
    46321 tgcaaaaagc agaagaacaa aaagttttag cttctcgaga agaagatttc gaaaaagcag
    46381 cttattatcg cgatcagatc aataaacttc aaaaaatgaa agaacgtcag ctcactgaag
    46441 aggaaacacc tgtgattacc gaaaaagata tggaaaaaat cgttgaacaa cggacaggta
    46501 ttccagtggg tgaattaaaa gaaaaagaac agacacaact gaaaaactta gcagatgact
    46561 tgaaagctca tgtcatcgga caagataacg ctgtagatcg agtagcgaaa gcgattcgcc
    46621 gaaaccgtgt tggattaaac aaacagaatc gtccaatcgg ctccttctta tttgttggac
    46681 caactggtgt aggtaaaaca gagcttgcga aacaacttgc ctacgaacta ttcggttcac
    46741 aagattcaat gattcgtttc gatatgtctg aatatatgga aaaacatagt gtctctaaat
    46801 tgattggttc tcctccaggt tatgtcggat acgaagaagc tggtcaatta acagaaaaag
    46861 tacggagaaa tccttacagt cttgtcttgt tagatgaagt agaaaaagct catccagatg
    46921 ttttgcatat gttcttgcaa atcttagatg atggtcgatt gacagatgca caaggaagaa
    46981 cggtcagctt taaagatacg atcatcatta tgacaagtaa tgccggaact ggtaaagtag
    47041 aagcaaacgt tggttttggt gcggcacgcg aaggtgttac tcgttctgta ttgaaccaat
    47101 taaacaacta ctttacacca gaatttctga accgttttga cgggatcatc gaattcagtg
    47161 cattatcaaa agaaaacttg atgacaatcg tcacactaat gctagacgat gtcaaccaaa
    47221 tgctggcagc acagcaactt cacatcgaag ttcctacgaa tgtcaaagaa aaattagttg
    47281 acttaggcta tgatccatct atgggtgctc gtcctcttcg acgcacgatc caagaacaaa
    47341 tcgaagatgg tatcgctgaa ttttatcttg atcacccttc tatccatgag ttgaaagcaa
    47401 aactggataa agatggaaaa atcatcgtta cttctaaacc agaacgtttg gctaaagaat
    47461 cagccgaaga aacggctgaa taaatccagt tttatagcgt gagacggtat ttctttgaga
    47521 aatgccgtct cattcttttt tcagaaaagt caaaatactg ttcattttat aaaaaagaaa
    47581 ttataataag agaaacaagc aattaaaaat acataaaaga ggtgagcgcc atgaaattgg
    47641 taaatgtaac aaacagccat tcccgtcttg tattaaacca gcttgaaaac actgatgccc
    47701 atctagtcaa ggtctacaca gccggaaata ccactattgt ctacactgaa gcccctgaac
    47761 ataatgaaat tctgctgatc aatgacaaac gtaaaatcca gccgaaagaa atcgaagatg
    47821 ctaaaaatta tttcttagat aaaatcaaag acgccgttta ccacgaggat gaaatcaaag
    47881 taatcgaatt ggcaggcctt gttgaaatct cgattccaaa acatgaatta ggcgctacat
    47941 tagccgtgta aacaaatttt gaacataata attaaagagc gtgggacaaa agtaaa
//
LOCUS       DAFBQW010000017        44558 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFBQW010000017 DAFBQW010000000
VERSION     DAFBQW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.017"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            13..1221
                     /locus_tag="LK889_001189"
     CDS             13..1221
                     /locus_tag="LK889_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBK5095437.1"
                     /translation="MNEDDTLLEVQNDKSVEEIPSPVTGTVKNVIVPEGTVANVGDVL
                     VEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKD
                     VDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPA
                     KPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEV
                     AAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLY
                     VPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVI
                     NYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLL
                     ADPELLMMEG"
     gene            1229..2635
                     /gene="lpdA"
                     /locus_tag="LK889_001190"
     CDS             1229..2635
                     /gene="lpdA"
                     /locus_tag="LK889_001190"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK5095438.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2797..3072
                     /locus_tag="LK889_001191"
     CDS             2797..3072
                     /locus_tag="LK889_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBK5095439.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3078..3845
                     /locus_tag="LK889_001192"
     CDS             3078..3845
                     /locus_tag="LK889_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBK5095440.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4013..5842
                     /gene="typA"
                     /locus_tag="LK889_001193"
     CDS             4013..5842
                     /gene="typA"
                     /locus_tag="LK889_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBK5095441.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6103..7875
                     /gene="recQ"
                     /locus_tag="LK889_001194"
     CDS             6103..7875
                     /gene="recQ"
                     /locus_tag="LK889_001194"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBK5095442.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7989..8291
                     /locus_tag="LK889_001195"
     CDS             7989..8291
                     /locus_tag="LK889_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBK5095443.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8294..9457
                     /locus_tag="LK889_001196"
     CDS             8294..9457
                     /locus_tag="LK889_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5095444.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9483..12911
                     /locus_tag="LK889_001197"
     CDS             9483..12911
                     /locus_tag="LK889_001197"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBK5095445.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12932..14056
                     /locus_tag="LK889_001198"
     CDS             12932..14056
                     /locus_tag="LK889_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095446.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14046..14483
                     /locus_tag="LK889_001199"
     CDS             14046..14483
                     /locus_tag="LK889_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5095447.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14467..14805
                     /locus_tag="LK889_001200"
     CDS             14467..14805
                     /locus_tag="LK889_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBK5095448.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14924..15484
                     /gene="rsmD"
                     /locus_tag="LK889_001201"
     CDS             14924..15484
                     /gene="rsmD"
                     /locus_tag="LK889_001201"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBK5095449.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15481..15972
                     /gene="coaD"
                     /locus_tag="LK889_001202"
     CDS             15481..15972
                     /gene="coaD"
                     /locus_tag="LK889_001202"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK5095450.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15962..17005
                     /locus_tag="LK889_001203"
     CDS             15962..17005
                     /locus_tag="LK889_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBK5095451.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17164..17892
                     /locus_tag="LK889_001204"
     CDS             17164..17892
                     /locus_tag="LK889_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK5095452.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18008..18253
                     /locus_tag="LK889_001205"
     CDS             18008..18253
                     /locus_tag="LK889_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5095453.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18250..18468
                     /locus_tag="LK889_001206"
     CDS             18250..18468
                     /locus_tag="LK889_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK5095454.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18604..19143
                     /locus_tag="LK889_001207"
     CDS             18604..19143
                     /locus_tag="LK889_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBK5095455.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19136..21409
                     /locus_tag="LK889_001208"
     CDS             19136..21409
                     /locus_tag="LK889_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBK5095456.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21447..22493
                     /gene="holA"
                     /locus_tag="LK889_001209"
     CDS             21447..22493
                     /gene="holA"
                     /locus_tag="LK889_001209"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK5095457.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22582..22836)
                     /locus_tag="LK889_001210"
     CDS             complement(22582..22836)
                     /locus_tag="LK889_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK5095458.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23236..23505)
                     /locus_tag="LK889_001211"
     CDS             complement(23236..23505)
                     /locus_tag="LK889_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095459.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23975..24430)
                     /locus_tag="LK889_001212"
     CDS             complement(23975..24430)
                     /locus_tag="LK889_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK5095460.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24568..26496)
                     /locus_tag="LK889_001213"
     CDS             complement(24568..26496)
                     /locus_tag="LK889_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK5095461.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26693..27526)
                     /locus_tag="LK889_001214"
     CDS             complement(26693..27526)
                     /locus_tag="LK889_001214"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK5095462.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27718..28422)
                     /locus_tag="LK889_001215"
     CDS             complement(27718..28422)
                     /locus_tag="LK889_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5095463.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28445..29071)
                     /locus_tag="LK889_001216"
     CDS             complement(28445..29071)
                     /locus_tag="LK889_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5095464.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29397..31772
                     /locus_tag="LK889_001217"
     CDS             29397..31772
                     /locus_tag="LK889_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK5095465.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31796..32287)
                     /locus_tag="LK889_001218"
     CDS             complement(31796..32287)
                     /locus_tag="LK889_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBK5095466.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32474..33394
                     /locus_tag="LK889_001219"
     CDS             32474..33394
                     /locus_tag="LK889_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5095467.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33548..33892
                     /locus_tag="LK889_001220"
     CDS             33548..33892
                     /locus_tag="LK889_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBK5095468.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34082..34582)
                     /locus_tag="LK889_001221"
     CDS             complement(34082..34582)
                     /locus_tag="LK889_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5095469.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35110..35193
                     /locus_tag="LK889_001222"
     tRNA            35110..35193
                     /locus_tag="LK889_001222"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35144..35146,aa:Leu,seq:cag)
     gene            complement(35517..36155)
                     /locus_tag="LK889_001223"
     CDS             complement(35517..36155)
                     /locus_tag="LK889_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5095470.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            36490..36924
                     /locus_tag="LK889_001224"
     CDS             36490..36924
                     /locus_tag="LK889_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5095471.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            36947..37267
                     /locus_tag="LK889_001225"
     CDS             36947..37267
                     /locus_tag="LK889_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095472.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            37408..37599
                     /locus_tag="LK889_001226"
     CDS             37408..37599
                     /locus_tag="LK889_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095473.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(37670..37927)
                     /locus_tag="LK889_001227"
     CDS             complement(37670..37927)
                     /locus_tag="LK889_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBK5095474.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            38085..38348
                     /locus_tag="LK889_001228"
     CDS             38085..38348
                     /locus_tag="LK889_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095475.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            38372..38560
                     /locus_tag="LK889_001229"
     CDS             38372..38560
                     /locus_tag="LK889_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095476.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(38643..38828)
                     /locus_tag="LK889_001230"
     CDS             complement(38643..38828)
                     /locus_tag="LK889_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095477.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            39043..39414
                     /locus_tag="LK889_001231"
     CDS             39043..39414
                     /locus_tag="LK889_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5095478.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(39564..39636)
                     /locus_tag="LK889_001232"
     tRNA            complement(39564..39636)
                     /locus_tag="LK889_001232"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39601..39603),aa:Gln,seq:ctg)
     gene            complement(39682..40110)
                     /locus_tag="LK889_001233"
     CDS             complement(39682..40110)
                     /locus_tag="LK889_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095479.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(40170..40928)
                     /locus_tag="LK889_001234"
     CDS             complement(40170..40928)
                     /locus_tag="LK889_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK5095480.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            41223..41420
                     /locus_tag="LK889_001235"
     CDS             41223..41420
                     /locus_tag="LK889_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095481.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            41526..42008
                     /locus_tag="LK889_001236"
     CDS             41526..42008
                     /locus_tag="LK889_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095482.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(42066..42662)
                     /locus_tag="LK889_001237"
     CDS             complement(42066..42662)
                     /locus_tag="LK889_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5095483.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            43049..44515
                     /locus_tag="LK889_001238"
     CDS             43049..44515
                     /locus_tag="LK889_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBK5095484.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        14722 a         7634 c         9116 g        13086 t
ORIGIN      
        1 ccaggagata cgatcaatga agatgataca ttactagaag ttcaaaatga caaatcagta
       61 gaagaaattc cgtcacctgt cacagggaca gttaaaaacg tgattgttcc agaaggcact
      121 gtagcaaatg ttggggatgt attagtagaa atcgatgctc ctggacacaa tagtgcaccg
      181 tcaactagtg cgccatcagc tgaagcacca aaagaaaaag tggaaacatc aggttcagca
      241 agtgtcgttg aagctgccga tccaaataaa cgtgtattgg ctatgccatc tgttcgtcaa
      301 tttgcccgtg aaaaagatgt agatatctca caagtaactg caactggaaa aggtggcaga
      361 gtaacaaaag aggacatcga aaacttcttg gcaggtggtc caagcagtgc accagcgaaa
      421 tcagaagcgc cagaagcggc agcaccgaaa gaagcagctc ctgcagcaga aagcaaacca
      481 gctgcaccag caaaaccatt caagtccaac ttaggagact tggaagaacg tgtagcaatg
      541 acgccaacac gtaaagctat cgctaaagca atggtcaaca gtaagcatac agctccacac
      601 gtgacattgc atgatgaagt agaagtgtct aaactatggg ataatcggaa acgatttaaa
      661 gaagttgctg ctgcaaatgg tacgaaactt actttcttgc catatgtcgt gaaagcttta
      721 acagcgactg ttaagaaata cccagtattg aatgcatcta tcgacgatgc aaaccaagaa
      781 atcgtttata aacattacta caatatcggt attgcaactg atacggatca cggactatat
      841 gtaccaaatg tgaaagatgc agaccgcaaa ggaatgtttg ctattgcaga cgaaatcaat
      901 gagaaagcaa aattagctca tgacggcaaa ctatctgcag aagatatgcg caatggaaca
      961 attacgatca gcaatatcgg atctgtaggt ggcggttggt ttacaccagt catcaactat
     1021 ccagaagtag ctattttagg tgtaggaacg attgcacaac aaccaatcgt aaatgctgaa
     1081 ggcgaaatcg ttgtcggtcg tgtgatgaaa ctgtcattaa gtttcgatca ccgtatcgta
     1141 gatggagcaa ctgcgcaaca agcaatgaat aatatcaaac gtcttttagc tgatccagag
     1201 ctattaatga tggaaggatg ataaacaaat ggtagttgga gattttgctg tagaattaga
     1261 tacagttgta attggtgcgg gacctggtgg ctatgtagct gccatccgcg ctgctgaaat
     1321 gggacaaaaa gtagcaatca ttgaaagaga gtatattggc ggtgtttgtt tgaatgtcgg
     1381 ctgtattcct tcaaaagcac tgatagctgc aggacaccat tatcaagagt cactagattc
     1441 tgaaatgttt ggagtaacta gtgaaaacgt caaactagat tttgcaaaaa cacaagaatg
     1501 gaaagaaaac aaagttgtcc atacgttgac ttctggtgtt ggtttcctat taaaaaaaca
     1561 taaggttgaa acaatcgaag gagaagcgtt cttcgtagat gatcacacac tacgcgtcat
     1621 tcatccagat tcagctcaaa cttattcgtt caataacgca atcatcgcaa caggttcccg
     1681 tccaatcgag attccaggat ttaaattcgg aggacgtgtg ttagattcta ctggcggatt
     1741 agcattgaaa gaagtaccta agaaatttgt gatcatcggt ggcggtgtga ttggtgccga
     1801 attgggcgga gcgtacgcaa acttaggagc agaagtaacg attcttgaag gctcaccaca
     1861 aattttacca acttacgaaa aagatctagt aaaattagta gaagatgatt tcaagaaaaa
     1921 aggcgtaaca gtcgtaacga atgcaatggc gaaagaagca gtcgataacg gtgacagcgt
     1981 gacagtgaaa tatgcagtgg acgggaaaga agaatccgtt actgctgatt atgtgatggt
     2041 cactgtaggt cgtcgtccga atacagatga tatgggctta gaacaagcgg gtgtcgaagt
     2101 aggagaacga ggattgatca cggtggataa acaaggacgt acgaacgtac ctaacatcta
     2161 tgccatcggt gatatcgttc caggggcggc attagcacac aaagccagtt atgaagcaaa
     2221 aatcgctgct gaagcaatct ctggcaaaaa agtagctgta gattataaag caatgccggc
     2281 agtagccttc actgatcctg aattggcttc tgtaggtatg acgatcaaag aagcaaaaga
     2341 tgctggtatc gaagcaaaag catataaatt cccattttca gggaacggtc gggcattatc
     2401 gcttggtaaa acagaaggat tcatccgtct agttacgacg atcgaagata acgtgcttat
     2461 tggcgcgcaa atcggtggag tcggcgcaag tgatatggtc tctgaattag ctttagcaat
     2521 cgaatcagga atgaatgctg aagatatcgc cttgacgatt catcctcatc catctcttgg
     2581 cgaaatcgtc atggatgctt cggaattagc attaggttta ccaatccata tttgatttga
     2641 ataatacagg cgaaacgcaa ctgaaaaatc agttgcgttt tgttttttag aattatcaat
     2701 aatattatta tgtaaactta aaaatatgac aacttcttgg tttattacca cttcctatgc
     2761 tacactgaag aggaatatga aataggagag ttgaagatgc aagaatatca gtatccactt
     2821 gatttagatt ggacaacaga cgaaatggtc attgtaatga agatgtggga agatctggaa
     2881 cgtgcgaatg aaaaagggat aaacaatgaa gcttttttga agacttatca gcagtttaaa
     2941 actgttgtca aatccattgg tgaggaaaaa cgattgggac gggaatttga aaaagcttcc
     3001 ggttattctt tatatcgtac agtgcagcaa gcaaaaaaaa atccaaacaa aactttgaga
     3061 atgaacgggt gagataaatg gaacagatga ttgatcatat caagtcctgg cttgaagaag
     3121 ctggtgagaa aatcaaatct tctcaacaaa cattaacaat ctcgcaaaaa tcaaatcgaa
     3181 cggatctagt aacaaatgtc gatcgaaaca tccagaattt tctaatcgaa aaaatccatt
     3241 cctttgatcc agaggcgaaa atattggcag aagaagaagg gtgtaatcaa ttatcggatc
     3301 tcgatggaag agtctttatc atcgatccta ttgatggcac cttgaatttt gtgattgaag
     3361 gggaaaattt ttgtattatg ctcgctgttt atgaatccgg cgttggaaag cttggtttca
     3421 tttatgacgt gatgagaaat gaactttttt ggggtggaaa aggaataggc gtgtataaaa
     3481 atgatgagcc attgcctaga ccagcaaata aatccttatc ggaaggctta cttggtgtga
     3541 atagttatct tttcggtcat aataaatatg gaatccgaga aattgcggaa agcagcatgg
     3601 ggacacgtat gtacggatgc gctggtcttg aactagtggc aatcttgaaa ggaaaccaca
     3661 ttgcttatat ttcaaacctt agcccttggg attatgcagc gggaaatgtc ttattggaag
     3721 agtttggtat ggtaacaagc ggtctttttg gtactccttt gacatttgaa aaaagggaat
     3781 actatttagc agggacttct tgtgcatatg cagaaatcgc agaccttttg aaaaaagaag
     3841 gctgaaaaaa aggtagaaat cattcacaaa aatgataaaa acgagcaaaa gattaatttt
     3901 ctcgaaaaaa tgaaaacatt ttactatatt tcatgaaaaa agtctgctat aattagcagt
     3961 cgagtaaaat ttgccctgtc ataaagaacc aataaaaaag gagttagtta cattgaatta
     4021 tagaaacgat atccgtaacg tcgcaattat tgcccacgtt gaccacggaa aaacaacatt
     4081 agttgatgaa ctattaaaac aatcagatac attagacgca catacgcaat tgcaagaacg
     4141 tgcgatggac tcaaatgcgc tagaaaaaga acgtggtatc acgattttag ccaaaaacac
     4201 agctgtagac tataaaggta ttcgcgtaaa catcatggat actccaggac acgcggactt
     4261 cggtggcgaa gtagaacgga tcatgaaaat ggttgacggc gttgtgttgg tcgtagatgc
     4321 gtatgaagga acaatgcctc aaacacgttt tgtattgaaa aaagcactgg aacaacatat
     4381 cacaccaatc gttgtcgtaa acaagattga caaaccttct gctcgtcctg agcatgtcgt
     4441 agatgaagtt cttgaactat tcatcgaatt aggagcagat gatgatcagt tagactttcc
     4501 tgtgatttat gcatctgcac taaatggtac gtcaagcttg tcagatgatc cagcagatca
     4561 agaaccaacc atggcaccta tttttgatac aattattgaa aaaatccctg ctcctgtcga
     4621 taacagcgac gaaccattac aattccaagt ttcattactt gactacaacg attatgtagg
     4681 tcgtatcgga atcggacgtg tatttagagg aacaatcaaa gtaggagacc aagtagcatt
     4741 aattaaatta gacggaacag ttaagaaatt ccgtgtaacg aaattatttg gtttctttgg
     4801 actaaaacgt ctagagattc aagaagcaaa agctggagac ttgattgctg tctcaggtat
     4861 ggaagatatc tttgtcggtg aaacagttac tcctgtggat catcaagatg cgttacctat
     4921 ccttcacatt gacgaaccaa cattgcaaat gactttctta gttaataatt caccatttgc
     4981 cggtcgagaa ggtaaattcg taaccgctcg taagattgaa gaacgtttga tggcagaact
     5041 tcaaacagat gtatccttac gagttgaacc aacaaactct ccagatgcat ggacagtttc
     5101 tggtcgtgga gaattgcatt tgtctatctt gattgaaaac atgcgccgtg agggatacga
     5161 attgcaagta tctcgtccag aagttattga aaaagaaatc gacggtgtaa aatgcgaacc
     5221 gtttgaacgc gtgcaaatcg atacaccaga agaatatatg ggtagtgtta tcgagtcctt
     5281 gagcttacgt aaaggtgaaa tgcaagatat ggtccataca ggaaacggac aaatccgttt
     5341 gacattctta acacctgccc gcggtttgat tggttattca actgaattcc tttcaatgac
     5401 acgtggttac ggaatcatga accatacttt tgaccaatat ctgccaatgc ttcctggtca
     5461 aattggcgga cgtcaccaag gggcgcttgt ttcgatcgat acaggaaaag caacgactta
     5521 ttctatcatg agtatcgaag aacgcggtac agtcttcgta gaaccaggta cagaagtata
     5581 tgaaggaatg attatcgggg aaaacagccg tgataatgat ttgactgtca acatcacgaa
     5641 agcaaaacaa atgacaaacg tacggtctgc tactaaagac caaacatctg tcatcaaaaa
     5701 accaaaacaa ttgacgttgg aagaatcttt ggaattcttg aatgatgatg aatattgcga
     5761 agtaacacca gaaagcattc gcttgagaaa acaaatttta gagaaaaatg ctcgtgaaaa
     5821 agcatctaaa aagaaaaaat aatctgtaaa aggatgaaga gagaaaccag cttgttataa
     5881 aaaatagctg gtttcttttt ttgtccaact cttttctgtg gagaagtcat ctttgtcaat
     5941 ggacataaat tcagaaaata cgccgaaaga cgtttaaaag acttttaaaa aacttatttc
     6001 tagagagttt ttgtttcata ataagataat gtttcacgaa aggagatcaa cttgtccttc
     6061 gaagaaaatc gaagtaaaat ggcaagagaa aggagcggga agatgaacga cttattaaaa
     6121 caatactttg gctatgatga gtttagacct ggtcaaaaag aaattatcca gaaagtcatt
     6181 gatcaggaaa atgtattagg aatcatgcct accggaagtg ggaaatcaat ctgttaccag
     6241 cttccagctt tactattaga tgggcttact gtggttgtgt cccctttaat ctctttgatg
     6301 aaggatcagg tggacgcggc gaatcaatta ggtatacccg ccacttttat caatagctcg
     6361 ttagatggtt acgaaacagc tagacgcttc caagaaatcg atagacagca gtaccgttta
     6421 ctttatatcg ctcctgaacg ttttatcatg ccggatttta tccaagcaat gaagcgatgg
     6481 aacgtccgta tgatcgctat tgacgaggca cattgtatct ctcagtgggg gcatgatttc
     6541 cgcccaagtt atttacagat ggcgaaccag ctagatcaac tgccaaatcg tcctgtaatt
     6601 gttgctttga cagctacagc gactgtgcaa gtagctgctg acataaaaag acttttaaaa
     6661 attccagaaa acaaccatat tcagactggt tttgaacgag aaaatctacg atttcaagta
     6721 attaaagatc aaaaaaaaga acaatatcta atcgaatatt taaagatcaa caaaaatcaa
     6781 tctggtatca tttatgcagc aacgagaaaa gaagtagacc gtctctatca tttgcttaaa
     6841 aaattcgatt tttccgtggg tagatatcat ggtggtctaa atgagaatga gcgaacagaa
     6901 atgcaagaag cctttttata cgatcgcttg cagctgattg tagcgacaaa tgcatttggt
     6961 atgggtagaa ataaaagcaa tgtccgtttt gttatccatt atcaaattcc tggctctcta
     7021 gaagcatact atcaggaagc tggtcgggcg ggaagagatg gtctttccag tgaagccatc
     7081 ctgcttttct ctcctcaaga tatccaagtc caaaagtttt ttatccagca gtcccaaaga
     7141 gaagaagggc aaaagcaaaa agaatatgaa aaattaaaag cgatgacaga atacgtgtat
     7201 attgagtcct gtcttcagca atacatccta aactattttg gcgagacaag cagtccgtgc
     7261 aaccgttgtg gtaactgttt agatgacaga gaaatagtag aagtaacgac agaagcacag
     7321 atggttcttt cttgcttaaa acgtatgggt gagaactatg gtaaacaaat gctgatgaaa
     7381 gtcttagcag gatcgaaaga acaaaagctg caagctttag gttttggaca tttatctacc
     7441 tatggactga tgaaaaacca atcgcaaaaa gaaacgatgc agttaattga atatttgata
     7501 tcgaatggct atcttctgac catcaatggt gagtatcctg ttttgaaagt aacagaaaga
     7561 gggatacaag tattgaaagg acaagaatct gtttatcgaa aggaacccaa gaaagtccag
     7621 cagctatcgg atgaagaaac cgatacacta tttgaagtgc tgcgggaact gcgaacggat
     7681 ctagcatcag aagcaggcgt tcccccgtat gtcgtatttt cagattccac attgaaagaa
     7741 atgagccgaa tacgtccatc aagcagattg gagatgcttc agataaaagg agttggccaa
     7801 agcaagcttg ataaatatgg cgaagccttt ttatctcgga taaaaaatgc ggactctaac
     7861 gttctggata agtagaaaga acgaatacgt aaaaatgaat aaaaacaact ggttaaatct
     7921 gttttttttg ctccttatga aaatcaaata tgttataatt aaggaattag aatgggaggt
     7981 aagcacagat ggattctttg tcgaaaaaac ttgcgatcca agtattaaat gatgaagcag
     8041 aacggatcaa acgattaatc cgaaatcaaa acaacagtct ctgtatctca caatgcaaag
     8101 ccttcgaaga agtggtagat acacagatgt acggcttttc ccggcaagtc ggttttgctg
     8161 tccgtatagg agtaatcgga aagaacgagg gccagctgat gttaagtgag ctagaacgag
     8221 aattgaatca gttatatagt gaggtttacc aagaaattta tgataaaaaa gaagttggca
     8281 aggaggaata gacttgccta agaaagtgaa aaaaaggcat ctattagact atagcatctt
     8341 gattccctat ttaattttat gtgtattggg attgatcatg gtatatagtt ctacgtcgta
     8401 tctattatta gaaaacggac aaaatccgtc cgcatcagtc attaaccaga gtattttttg
     8461 ggtgcttagc ttgattgcta tcgcactact ttataaaatg aaaacagatg ttttgaaaaa
     8521 ccagcgtttg atcatggcag ccatcgcagt tttgacgatt ttgctgctga ttgttgtgtt
     8581 ttttggtaag gagatcaatg gggcgaaagg ttggctgcaa attgcaggat tctcgatcca
     8641 acctgccgaa tacctaaaaa tcatttctat ctggtacctg tctctgactt tatctaaaag
     8701 acaaaacagt gtacaaaaag attttcttgg gactgtgaaa cgtccacttg caatggttat
     8761 cggacttact gcattagtcg ctattcttcc cgattttggt aatgcggcag ttatcttttt
     8821 gattattttg gttctgcttt tagccagcgg agtcaattat gtgtatactt tgattgtagg
     8881 agtaggaggc ttttgtctaa gtacttttac gatctggctg atcaatatca caaacggaaa
     8941 gatcttaccc ggacgtttac agtatatcta taatcgattt gctatttacc aaaatccttt
     9001 ctcagacgaa ttaaataaag ggcatcaact agtcaacggc tactatgcca tgttcaacgg
     9061 aggactattt ggcaggggat taggtaacag tatccagaaa aaaggatttt tacaagaagc
     9121 acaaaccgat tttatttatg caatcgtagt ggaagaactg ggagtgatta tggggatttt
     9181 gatattagcg ttgctattct ttatgattgt gcgcattgtc cttgtaggta tccgttcaaa
     9241 agatccgttc aattcgcttt tgtgcattgg gatcggcgca atgtttttga tccaagtatt
     9301 tgtcaatctt ggaggaatta ctggcgttat tcctttgaca gggatcacgt tcccattttt
     9361 aagtcaagga ggctccagtt tgctgatgct ttcgatttgt gtcggctttg tcctcaacat
     9421 cagtgcggat gaaaaacgca agagcttagg tttataataa aaaaagatgt aggagtggtt
     9481 cgatgaaaaa agttttagtt gctaacaggg gtgaaatcgc tgtacgtgta tttcgtgctt
     9541 gtacagaatt aggaattaaa actgttggaa tctatgcaga agaagatgaa tattccgtac
     9601 accgtttcaa agcggatgaa gcatatttag taggacaagg aaaaaaaccg attgatgcgt
     9661 atctagatat tgaagggatc atttctatcg cgaaagaatg cggagctgac gctattcatc
     9721 cgggttacgg tcttttgtcg gaaaacttga attttgcgca acggtgcgaa gaagaaggca
     9781 tcatttttgt tggacccaaa ctgcatcatt tagatatttt tggagataaa atcaaagcaa
     9841 aagctgcagc tatcgaagcg gggattgctt cgattccagg cacagatgga ccaatcgctt
     9901 ctatagacga tgccttggag tttgcgaaac aatacggtta tccgattatg atcaaggcag
     9961 ctctaggcgg cggcggacgc ggcatgcgtg tagctcacga tgaaaagagt gcaagagagg
    10021 gctacgaacg tgcgaaaagc gaagcaaaag cagcctttgg tagtgatgaa gtgtatgttg
    10081 aaaaatacat cgctaatcct aagcatatcg aagtacagat tctcggggat acgcatggaa
    10141 acgtcatcca tctttttgaa agagattgtt ctgttcagcg ccgtcaccaa aaagtagtag
    10201 aagtagcgcc ttgtgtctct atgaatgaac agcaacgtca aaaaatctgc caggcagctg
    10261 tccaattgat gaaatatgtc gggtatgtca acgcaggtac agtagaattt cttgttgaag
    10321 gagatgattt ttactttatc gaagtcaacc ctcgcgtcca agtggaacat acgatcacag
    10381 aaatgattac agatattgat atcgtaacaa cgcaactgtt gattgctcaa ggtttggatc
    10441 tgcataaaga aatcggattg cctcagcaag aaggaattaa attaaatggt tcagccattc
    10501 aatgccgaat cactacagaa gacccgttga ataacttttt accagacaca gggaaaatcg
    10561 atacgtatcg ttcacctggt ggttttggcg tacgtctgga tgtcggaaat gcttacgcgg
    10621 gctatgtagt gacaccgtat tttgattcat tgctagtaaa agtatgtacg catggtgcta
    10681 cctttgaaac agccattcag aaaatggaac gttgcttgaa agaatttaga atccgtggag
    10741 tgaaaacaaa cattccattt atgctcaatg tcatcacaca tcctgaattc caatcaggaa
    10801 atgcaaaaac aaccttcatt gatagtacag caacattatt tgaatttcct cgactacgag
    10861 accgcggaaa caaaacgatg aaatatatcg gtgaaattac tgtgaatggt tttccaggta
    10921 tcgaaagtgg cgaaaaacca ttttatgaag aaccacgtat gcctaaagat ctaatcacaa
    10981 gaactgatta cgtaacggct aagaatgtat tagatgcgaa gggtgccgat gctttggtcg
    11041 aatggatcaa aggacaggaa aatttgttgt tgacggatac gacatttaga gacgcccatc
    11101 aaagtctgct agcaactcgt gttcggacaa aggatttcaa gcagattgca cgtttgactg
    11161 gagaaggtct tcctgaactc ttttcaagtg agatgtgggg gggagcaaca tttgatgtgg
    11221 cttaccgctt tttgaatgaa gatccttggc aacggttgag aaaaattcgt tctttaatgc
    11281 ctaatacgtt gctgcaaatg cttttcagag gttcaaatgc cgttgggtat tctaattatc
    11341 ctgacaatgt attggtggaa tttgtcaaag aagcagcagc gcaaggaatc gatgtatttc
    11401 gaatctttga tagtttgaac tggactccac aaatggaaaa aagtatccaa gctgtacggg
    11461 atactggtaa aatcgcagag gcagctattt gctatactgg cgatatcaac gatccaagtc
    11521 gggcaaaata caatgttcag tattataaag acatggcaaa ggaactagaa caactgggtg
    11581 cgcatatcat tgcaatcaaa gatatggctg ggctgcttaa accgcaggca gcttatcgtt
    11641 tgattagtga attgaaagaa acgacagatc tgccaatcca tctgcatact catgatacca
    11701 gcgggaatgg aatcatcact tattctgctg cttcaaaagc aggagtagac atcgttgacg
    11761 tggcaatgag tgcaatgagt gggaatacta gtcagcctag catgagcagc ttgtattatg
    11821 cactggtcaa tggtccacgg ctgccagaaa tcacgattga aaatgcacag aaattaaacc
    11881 attattggga agatgtgcgc atgtattaca aaccgtttga aaacggcttg aatgctcctg
    11941 aaacagaagt atatatgcat gaaatgccag gaggacagta ttcgaatcta cagcaacagg
    12001 caaaagctgt cggattaggt catcgctggg atgaaatcaa acaaatgtat catactgtca
    12061 acttgatgtt tggcgatatc gttaaagtaa ctccatcatc taaagtagtt ggagatatgg
    12121 cactatttat ggtccaaaat gatcttacag aagaagatat ttatgaaaaa ggcgaaacac
    12181 taagtttccc tgaatctgtt gtgactttct tccaaggaga actagggcaa cccgttggcg
    12241 gatttcctaa aaaattgcaa aagattattc ttaaaggcag acctgcattg aatgaacgtc
    12301 caggtctttt ggctgaatca gtcgacttca acgaagtaaa aaaagaattg gcagaaaaaa
    12361 ttggctatga accaaaacaa gacgaagtat taagctattt gatgtatccg caagtctttt
    12421 tggactatca aaaagcatat aatcaatttg gcgatgttac attactggac acaccaacat
    12481 ttttccaagg tatacgtttg ggtgaaacaa tcaatgtaca aatcgaacgc ggaaaaacgc
    12541 ttatcattcg tttagatgaa atcggagaac ccgatatcga agggaatcgc gtactgttct
    12601 tcaatttgaa tggacaaaga cgtgaaatca ctatcaaaga taattcgatc atcagtacgg
    12661 tccaaacccg attgaaagct gaaccgacaa accgagaaca aattggagca acgatgtctg
    12721 gttctgtttt ggatgtatta gtgaaaaaag gcgataacgt taaaaaaggt gatacgttga
    12781 tgattactga agcgatgaaa atggaaacgg ccatcgaagc acgttttgac ggagaagtcg
    12841 cacatgtata tgtgtcttca ggagacacta tttcgtctgg tgaccttttg attgaagtga
    12901 ctgagaaata ataagcgaaa ggtggggaaa catgaaaaga ctgggtttgt tttggctgat
    12961 tctgctatta gttattgtgg cattctacat ggaaccagtc ttgtttccgc caaaacttga
    13021 agtgaaggaa aactctgatc aacaaatatc aaaccaaatg acaacgtgga aataccagga
    13081 gcttaaagca actggttttt ctacttatat cggaaaaccg atcgatcagc tggaaaagga
    13141 ttttggccaa gcctatgatc gattaactag cggatttggt tttgaaacaa gatattatga
    13201 aaataaagct agtcatctca catttgaagc gaacatccaa gacagcagag tcacaactgt
    13261 taaagtactg caaacagggc ctgctgatat cgcgccgttt tatttaggga tgacgatgca
    13321 agatcttacg aaaatcacta ctatctatac taatttcact tttgagtacc atgatacaga
    13381 agttggaatc gaactaatgg aagaggatat gaattatcgt ccgctaattg cttttgataa
    13441 cgacactttt gctattttat tttttgatca aacaacaaac ggcctatttt cagttgtgta
    13501 tttagataaa gatagtcttt tgaaactatt gccatatcaa gtgtttggtg aaggccttcc
    13561 acattatcaa gcagataaat cggctgattg ggaatcaatc aatcgcgtta aagaacgaaa
    13621 aagtacgact ttattaaata tgcttagaag agaagacgaa cttcccatat acaatcaaac
    13681 acttgccttt caaaaccaca gtcagctgct actacatgat tttttgacaa ggcctgagga
    13741 aatattgtca gaggaacgta tggctgattg gcaaaaatcg ctaggcagtg ctcaaacgac
    13801 agtgaaattc tcactgacga atgatgaatt ggagaagtta atcagtaaac aaaaattaga
    13861 aaatactagt gggctattta ttcatccggc gattgacgct actttttcgt ttttatattg
    13921 gtacagcgat ccttacaacc atgaccgttt catggacccg ggaacggatt ctgtaggcat
    13981 tgctttttct aaagaaaata tgttagtctt attgcaagaa gaaaacggag aaagtagtga
    14041 tagcagtgat cgttaacgaa gagctgtttc aactggagga tcaatgcctc caagtcgctg
    14101 aaatgataaa aaagagtaaa acgatggctt cttatcttga acataaacag tcaatggaag
    14161 aatctgcaaa agtttgccag ttgaaagaag cgttcttgtc taagaaagaa gcatttgaaa
    14221 atgtagcggc atatggtgag tatgcacctg attatcgtga aaaaaaacga gctgttcgaa
    14281 aagcaaaaag acagcttgat atggatgagc atgttgcagc tttccgatat agtgaaacac
    14341 agctgcaaag tctgttggat caaattgtcc aagtacttgc cgaagaagtt tctaatgaaa
    14401 tcaaagtaga tgcaggtagc ccgttttttg aaacgaaaaa acactttgga tgcggaggta
    14461 attgtcatgc aattagacaa tagtgaattt gaaataacaa aacgacgcgg gctgatcgta
    14521 tgggtctaca gcttaaaaca attaaagaat ctgaaaaagt tcggcttgtt gcattatgtc
    14581 tcaagaagaa tgaaatatgt cgtgctttac ttaaatgaag agacattgga agaaacagaa
    14641 gaacgaatcc gtaaattaca ttttgttcga aacgtagaac gttcttaccg accggatatc
    14701 gaaatgaatt ttgccgaaaa aataggaaac aaaacagtat taaaagacga aggtttcgaa
    14761 attgaagagc taaatacaga aatccgatta gctgaacatc tttaaaaatc tcttatgagc
    14821 agtttttaaa taaaaaataa cttggataac gatcgatttt tgatcattgt ccaagtattt
    14881 ttttggtata ctagtaccag taccatttct aaggagtgag tagatgcgga taatatcagg
    14941 aaattatggc gggcgccgtt taaaaagttt agcaggggcg aacaccagac caactacaga
    15001 taaagtaaaa gaatccatat tcaatatgat tggaccatat tttgatggag agacagtatt
    15061 ggatctgttt gcaggaagcg gtggtttagc tatcgaagca gtatctagag gctgcagtca
    15121 tgcggtatgc gtagacaaaa attaccaagc gctaaaaatc atcaaagaaa acatcgaaat
    15181 tacgaaagaa ccagagaaat ttaccacaat aaaacttgat gcggacaaag caatcgaacg
    15241 gctagcagcg gagaaacaac aattcgacta cttgtttctt gatccgccat atgcaaaaca
    15301 aaaaataatc gatcaaatcc ataaaatgga agaactggga cttttttcta aaaccgcagt
    15361 tatcgtatgc gaaacggata aaacagtcga attgccagaa actgtggctg gattccatca
    15421 aataagaaaa cagatttatg gcataacggc cgtaactatc tatagaaaag agggaaatca
    15481 atgaaacgtg cattattccc aggaagtttt gatcctttta caaaaggtca cttagatacg
    15541 gtggaacgtg cggctaaatt attcgatgag gtagtaattg gtgtttttat caatacttca
    15601 aagaaaagtt tgtttccccc agaagaaaga atgacattga tcacaaaagc tgtttctcat
    15661 cttcctaatg taaaagtcat gcatcaagaa aatcaattga cggtagaaac agcaaaagag
    15721 attggtgcag atgcgttgat cagaggcata cgaagtatca aagactttga atatgaacga
    15781 gagattgctc agatgaatca tcatctatat gatgaattgg aaactgtctt cttattagca
    15841 aaaccagaat atagtcacgt gagttccagt attttaaaag aagtactgca ctttggcgga
    15901 gatgtgagca gctacttacc gccagtgatc aacgaagctt tagctggaaa gcgtgaatca
    15961 aatgaaagct aaacaatggt tgaaaacagt actgattatc atatttggaa tgttgatcat
    16021 tggatttgta gtagttccct tgccgtatta tgtagaatca ccgggagcca cgatcgatct
    16081 gaaagaacta attacggtca atgataagga agataaaggt tctgggtctt tctctcttac
    16141 ctctgttggt atcaggcaag ctacaggttt cttagcttta aaagctaaaa tgaccccttt
    16201 tgaagaattg atcagtgaag aagaattgac tggtggggcg acaaatgaag aatataatca
    16261 aatgcaacag tattatatgg attcttccca aaatgcagca atcgaacaag cgttaaaact
    16321 tgcagacaag ccttataaaa tggaattcaa aggcgtatat gtcatgagtg tagagaaaac
    16381 atcgaatttc tttggcaagc tttcagttgg agatacagta acaaaagtag acgggaaaag
    16441 ctttcaaagt acagaagcat ttatggatta tgtaaaaagc caaaaagttg gacaaacaat
    16501 cgaaatcagt tatttgcata atggtgaaga aaaaacagct tctggtgatc tgatcgagtt
    16561 accaacagat aaaaaggcag gaataggtat cacgcttact gaccataccg agatcgaatc
    16621 agatacagat gttcgtatta attctggctc tatcggtggg ccatcagcgg gattgatgtt
    16681 cactttagaa atctatgaac aagtcactgg taaaaacttg cgacatggta agcaaattgc
    16741 aggaactgga acgatcaaca gtgaaggaga agttggccga atcggaggaa tcgataaaaa
    16801 agtggcaagt gcggataaag ctggcgtaga gattttcttt gcaccagatg atacgatttc
    16861 agacgatgtt aagaaagaat atcctgaaat aaaaaccaac tacgaagaag caaaagctgc
    16921 cgctaaaaaa ttgaattcct caatgaaaat cgtaccagta aaaacagtac aagaagcgct
    16981 tgactatcta gagaaaatga aatgaaacta aataattaaa gaggggttgt gacacgagtt
    17041 ctgtcacaac ccctcttttt cgtcaaaaat cttcacgata aaaataaaaa caaaagaaaa
    17101 aattgacgta attagatgtt gaagggacga ttttgccaga gctcactgtt tcttcaattt
    17161 taaatgaaag gagtaatgaa tgatttttac tggtcattct ttgaaaaaag aacaatgata
    17221 gaaaaaatca gcaagaaaat actagcttca cccaaattga tcatttggat gtctatgata
    17281 gttcttatct tttgtatcgc aagtggtttt ttattatcaa atttgctatt ttatagagaa
    17341 acagaagaga ctgctgcaaa agaaatactt tcttcgttta gttccagtgg agaagatcca
    17401 tcagctgaag gaaacgatgt agaagaggat gctcgtccaa aggtcatgta caccgatatc
    17461 aaaggaagtg tgaaggagcc tggtatctat tccttttctt cagaagaacg ggtctatgat
    17521 gtactaaagc gtgcaggtgg attattagaa gaagcggata gcgatcgaat caacttttct
    17581 gcgaagatag aagatcagca agtcctatat atccctgctg taggtgaaga gctgccagaa
    17641 catcttgatc agttggcatc ctcggaagga aaacagccta cagcagattc cgagcctccc
    17701 aaaataaata tcaatacagc cactccatca gaacttcagc agattcctgg gatcggatca
    17761 gtcaaagcgc aagaaatcat tcgatttaga gaagaaaacg gatccttcca gaaagtggaa
    17821 gacctgcagg agatttctgg tatcggtgaa aagacagttg aaaaactaaa aaacttcgtt
    17881 acaattaaat agaatgattc aaaatcgact ggcacgagca ataaatggac gctaatggat
    17941 actaagaata atccaattct aagagcttgg caggacgagg aggtagtatt tttggatagg
    18001 aaatagcttg attgttcgca aagaaacatt aaaaaaacca atgctgaatg tctatctcca
    18061 aaataaaata tctgggattc atattatgaa tacggctgtc agcggcaata acagtcaagc
    18121 tctacgagaa cgctttgcca aagatgtact tagctataca gcagataaag tatttattct
    18181 cattgggacg aatgatctag cagagcataa acaactttct aaagaaacgt accaaaaaat
    18241 ctgcagtgga tgatagttca gctgaaaaaa gactatagtt ctgatcatgc tttctttatc
    18301 acgccaccag cagtcgatga gacaaaacaa aaatatcgga ataacaagtt gatcaaagaa
    18361 tatatctctg ttctcacaga agtagcaaaa cgccatgatt gtgaagtatc gatttttata
    18421 cggaatttct tgaacaaatg gattcgaaga atttttataa tgggctaatg gatgacggac
    18481 ttcatttcgg cagtgaaggg taggatcttt tgtcagacag gatgattcgt tatatttaaa
    18541 gaatattttg tttaagcaat gagtactttt taatcaactt gaaaaaacgg aggaatataa
    18601 aacatgttag aaaaatcttc aaaagcatgc gatacgaata aagaaaaagt tactgataag
    18661 tcacataatg aacgcattcc ttgggaccag tactttatgg ctcaggcagt actgttatct
    18721 ttacgtagta cgtgtacacg tttagaagta ggagcgacac tcgttcgaga aaaaaggatc
    18781 attgctggcg gctataatgg agcagtcagt ggagatgtcc actgcataga tgaagggtgt
    18841 tacgtcgtgg atggacactg cttgcgaacg atccatgcag agatgaatgc actcctacag
    18901 tgtgcaaaac ttggtatccc gacggaaggt tcagaaatct atgtcacaca ttttccatgt
    18961 ttggcctgta caaaggcttt gttgcaagct ggtgtgaaag ggatcaatta tctccatgac
    19021 tatcgaaatg atccttatgc ccaagcgttg atcgaacaat taggtgtatc tgtacaccaa
    19081 gttgctttag actcacagca tttttcaaaa cttcaaaatg aattaacagg aaaacatgtc
    19141 tagcgtttta gaaaggcatg ttcgaaatcg gttgattttt ccggcaatta cgtcgatgat
    19201 tatctgtttg ttgtattaca ctgacaattg gggttatcgt ttgcttcttg ccggaatttt
    19261 tttatggttt tcgctaaaag aaggacgttt tatcgcgtac ttgtgtattt tgtgtagcat
    19321 aacggtatcc atttcttata ggctacaaga gtattcattt ttagaggaaa aaacaacatc
    19381 tgcgcaaatt actgtattta cagatacgat tcgtgcaaat ggtaattttt tgacaatgga
    19441 aggaaggtta tcagagaata aacaaaagct ccagctttct tatcaagtaa acacagaagc
    19501 agaattgaaa acatggctta acttccgagg aaagggaata gaattgactg ctgaaggcga
    19561 gttcatctct ccagcaaaaa agcggaatct ttataatttt gaccaagaag cttacttcag
    19621 gaaccataga atttctggac ggttccaaat tcaaaagatt gaacagatca ccatgcaaaa
    19681 tcactggcga aactggcttc agagaaaaag aggatctttt atctcctttg tccaatctga
    19741 atttccgcaa aaaacaagtg tatatattaa ttctttatta tggggctata aagatgcagg
    19801 attcagagaa ccagaagaaa tttttaggga gagtggtttg ctgcatctgt tcagtctatc
    19861 gggtctacac atccaatttt atttagggtg gctgtattat ttatttcgac gtatcggatg
    19921 gccactacac gtcagtatta ttccgttgag tctactaact atctgttatg tttctattgc
    19981 aggaagttct atttctgttt tgcgtgctag tttgctgttt ttattgcgat tgctagtcaa
    20041 attgctgaat aagcattatt ccaccatgga cttatttgca gttgtctttt ggcttctttt
    20101 actatttgat cctcgatttc tttttcatgc gggtggacag cttagttttt tcatgagttt
    20161 tttgtttatc ctgatttctt ttgatggaga ttggttgaaa aaaacggcct ctcacgtcct
    20221 gtttactatg atcacgatgc cgttgattgt atggcatttt tatgagtggc ctattttagg
    20281 tagtcttttt acattactga ttgcaccggt attcaagttt ctttttcttc ctggtgtgtt
    20341 atttctcaca ctcttcaatc gctggctgcc ggaaacttta cttttgttga tagaagaggg
    20401 attaatctta tttgagcgaa taatcgattt ttcttcaggg tcgtctctca ttataggacg
    20461 tttgcccctt gtgctatctg ggatgtatat tttcggatta ctgatttgta tggataggtt
    20521 gaaagaacaa cctgtgaaac acttgtttta tgttggtctt ttttctttta tttttttgac
    20581 tttgccattt ttacgctttt cttcaacgat cgcttttatc gatgtcggtc aaggagacag
    20641 tatctttttg caaagtcctt ttcgaaaaga aacgattttg atcgatactg gaggtcagtt
    20701 acactttgaa agagaaaagt gggcgcaagg tatgatacgt cctcctgctg aaagaaatat
    20761 cgttccatat ctaaaaggtc aggggataag tgtaatcgat aaactcatct tgacacacga
    20821 cgatacggat catgtgggag aattacccac gttatctcag cattttacta tcaaaaaaat
    20881 ttatatcggt tggggcgctg gacgaaatga gccattacaa aggcatttat ctgaagctca
    20941 aaaaaatggt actgagatca ttgaaatcaa acatggggac aggatcagcg gttattatga
    21001 tctttatgta ttgactcctt ttgaaaaggg agagggaaga aatgaagatt cgattggtct
    21061 atggatggaa tataacaata ggcgcttttt atttttagga gatttgaatc aagcaatgga
    21121 aaaacaattg cttttgattt atcctaatct gaaagccgat gtagtcaaat taggtcatca
    21181 cggtagccgt acgtcaagta acacagattt tctttcccat attgaagcaa aacatgggat
    21241 tatatcctgc ggcgtaaata atcgttatgg tcacccgcac agcgaagtat tggagacact
    21301 agaagaaaat cagataaaaa catggcgaac ggatcaacaa ggaatgatca cctatcgctg
    21361 gcatccagtt tttcatcctc atgggctagt ggaaacaatg atagactagt aattctcatc
    21421 tgctcatggt aggataattt ttgaaagtgg tgagtaattt gagtatacaa gcagagttac
    21481 aaaaaatacg taaagaaaaa cttgcatctt gttacttgat tttaggaaca gaaaagtttc
    21541 tccaagatca agttcggaca gaaattgtca aaaaaatcca attagagggc gaagatgact
    21601 taaatttttt aagttttgac atggaaaatg caagtttaga tgaggtagta gctgaagcag
    21661 agacgttgcc gtttttcgga gatcaacggt tagtattcgt agagaatcct tattttttaa
    21721 caggtgaaaa agtaaacaat ggaatcgaac aaaatactga tttattgacg gcttatttga
    21781 aagaaccatt agaatcgact gtattagtat tctttgcgcc atatgaaaag ttggatgaac
    21841 ggaaaaaagt aacgaaacaa ttgaaaaaaa cagcaattat catcaatgtc caacagttga
    21901 atgaaaaaga agtacgtcaa tatctgatga ataccttaga gaattcctca atcaaaatgg
    21961 aacgaagtgc aattgattta ttcttgaggt tgaccgactt agatttatcc aaaatgatga
    22021 gagagttaca gaaattggtc ctttatggac aaaatcaaca gcaaatcacg gtaagagaag
    22081 tagaagaatt agttcctaaa acgttagaac ataatatttt tgacatgacc caatatattt
    22141 taaaagggaa gacggaacaa gctttgcgtt tatacgaaga tttagttatg caaggggaag
    22201 aaacaatcaa aattaatgcg attttgcttt ctcaattacg tttattttta cagacaaaat
    22261 ttcttgtaaa gattggttat cagcaggcaa atattgctga aacattgaaa atccatccat
    22321 atcgtgtaaa acttgccatg caggaagtaa aaaaattcga tgaacatttg ctggtacagt
    22381 tgtttgatca attagttgag atggattatc aaatcaaaac gggacaaatc gaaaaagaat
    22441 taagttttca actattcgtt ttgcgcacag ggcagttgtt aagaaatcga taagaagaaa
    22501 gacgagggca aagcatgcgg gaaggtcgtt tgtttttgtc ctttttttga caaataaaaa
    22561 aatcggccga agccgattct tttatttagc gatttttttg cttaaacgag atttgtcgcg
    22621 gttagctttg tttttgtgga ttagaccttt tgattcagcc atatcaatag ctttaacagc
    22681 ttctttatat aacgcatcta cgttgtcagc acctgaagct acagcatctt caaatttttt
    22741 gattgcagta cgcatagcgc ttgtttgaga tgaatttttc acattagcgt tttcgcttgt
    22801 acgaacacgt ttgattgcag attcaatgtt tggcattagt ttcacctccg ataacttaag
    22861 ttcttacgcg tgttaccgca taattcaaca ttcattatta tacctaaagg cagcgtgctt
    22921 tgcaatatga acatgacaag tttttttcct taacagtctt ttattaggat tttttttcag
    22981 taaaaacgga tagtacaagc tgaaaaaaat caaaaagacg ctattgtggg aggcaatagc
    23041 gtcaaaccaa actagatttt tatttatcta tttaaacaaa gtttgaatgg gaatgtttgg
    23101 tactaagtta ttatataaca aagagaaccg tttgcaaagt tttttttgaa aagggatacg
    23161 attccctgtt gttctttgtt tttctattaa gaaactgcag ttattttata accagtatcc
    23221 tttgatcagt aaaggctatt ctggtgtacc gattatttta atgtattcat cgatattttt
    23281 tatcaacatt ttttttatga ccaactggtt ccgacggttc catttgccac ttacttccag
    23341 cacatattta tttaggggca aacgtaagat atattttgcc aaatagcgat ctttaagcag
    23401 gccattgatc acacggtcct caagctgcaa gttgatccca gtcagcatca ttggctgttc
    23461 ttgtaaaacg tacgtatcaa ttaaaagacc ttcaaaagtt tccacaagcg tcttcctttc
    23521 ttttcaaagt aacctgaaaa caggcactaa ttcttccatt ttgatattcg ttgaagtgga
    23581 tgcgatcatt ctttcttttg tccactgtaa tctagtataa caaaaaagag cagaagagcc
    23641 aaaaagaaaa aagagcatcg aattctatag gaagtctcat tggcacggcc taaattatta
    23701 caaaacattg attaaaaaaa gcccctgcat cgcaggagca tcccctatat cgttttaata
    23761 ggtcaatcaa aaagcagtat caaacacatt ttgtatcaaa tcaggaaata atagataagc
    23821 taagcccaga atcaaaagta ccgctagaag aaaccagccg attgttccta tgaaatactt
    23881 taatttttcg aacattgtat cacctcgttg tcagtatatc acggatcagt aaattggggg
    23941 tgttaagaat ctcctaaaat agacaaaatt tgttctattt tcctaaagca aatacttgta
    24001 agatacagta agtgatacca gaagcaatga ccggtccggc ggcgattcct cttaagaaga
    24061 caacacctaa aatcgtgccg aaaacaagtg caacagtgat ttgtggatca gaagcaatca
    24121 atcctacacc cttggaagat aagacagcca ctagcccacc gcaaaaaaca gctacccagc
    24181 caatagggga tttaaaggct tctaatagat cacgtagacc gatttctcct gttgcgatag
    24241 ggattagaat agccgctgtg atgatcgtca ccccaatatt gattcctttg ccttgaagcc
    24301 aagggaaaaa ggaacgggag gctggtatga gcttcataat caaaacaaaa gctgccgcta
    24361 tcatcagact ttgattttta gctaaaattg ctacaagtaa aataattcct aaaaaaagcc
    24421 aactttccat gaaatatatt cctcactttc ttcgaaacaa tcataacatg tttttagaaa
    24481 gttacaaaag taggaattca tccttcaaga cctattcata agctgttttt ttcataaaat
    24541 aaacaaaatc ataaaaaaac actttgctta tttgacagaa gacagccgcg ctttgtatag
    24601 tggacaagac ttacattttt ttgaagaatc agaaagatca ggtaaaggtc ttagtaaacg
    24661 caagccattc agaataacca atattgtgct tccttcgtgt cctacaactc caagaggtag
    24721 atttaaaaat tgcaggaagt tagaagcaat caacaagaga ataacaagaa tcgaaaaaat
    24781 aatattttgt ttgatgatcc gccgcatttt taaagatagc cgatgactgt agacaaattt
    24841 atttaagtcg cttttcatta agacgatgtc tgcgatatcc atagcggcat ctgtcccttg
    24901 ccccatagcg acacctaaag aagcggctgc taaagcaggc gcatcattga ctccatcacc
    24961 aaccatcgca tttacttggt aaagttcatt ctcttttttg actagttgtg ttttttcttc
    25021 cggagtacag cttgcatagt aatgttcaat ccctacttgt tcagcaatgg cctttgctgt
    25081 gccagaatgg tcgccggtta tcatacttgt atggatgttt tgggatttaa agtaggaaat
    25141 tacttgttgg gtatttgctt ttggaatatc tagcagacct aaaacagcaa ttaactgctg
    25201 atctttagaa agataaatca cggttttgcc ttgattttcc aaacgttcga tcatttccgc
    25261 aatctctttg ctgattatca tcgtctcagg atcatatgcg tgcttgccaa ctttccattg
    25321 cgcacctaaa taggtagttt gtaatccaaa accagtaatt tcttctattg gtaagtattg
    25381 gatttcttca ggtattgtgc aatcgaaata ttgcaaaatc gcatgagcaa gtggatgcgt
    25441 cgttttttgt tccatagcaa caagaagttg ttgtgccaaa tccttttcat caaagaaaaa
    25501 gctgtcagtt acaactggtt ttcctttagt gagcgttcct gttttatcaa aggcaatcgc
    25561 ttttaattca gaaagctttt ctaaatggat accgcctttg attaaaatcc cattttttgc
    25621 agaatttgaa agagcagcta gagaagctgg tgtagcagat gcgacaagcg cacaaggaga
    25681 agccacaact aataatacca ttcctcgata aaagctttca tcccaactcc aacctaggaa
    25741 aaagtggggg agaaggatca ttagaggaac agcaagcaaa atcaatttta cataattatt
    25801 ttcgattcgt tcgataaatg aagctgtttg agttggcgta ttctgcgctt cttctactaa
    25861 ttggataatc ttagcaaaga cagtttcgga actcgttttt gttactcgaa tcgtgatggg
    25921 atttccttga ttcagcgttc ctgcatacat ctcatcgcct aaatattttt ctacaggcac
    25981 agattcaccg cttaatgcag actcatcgat cgttgcctgt gtgctttcta aaaagccatc
    26041 tatcggaaca gctgctcctt tagcgacaaa taccaagtta tccaccttta gttgagaaac
    26101 atctacttct tgcatttgtc cattttcttt taataattgg gctttttgtg gttgaagatt
    26161 catcaaagca gtaatttctt tttgactttt attagttgtg tattcctcta atgcaccgct
    26221 taaacaaaag atgaatgtca acattgctcc ttcaaagtaa ttaccgatca aacaggcacc
    26281 aatagcagct aaagccatca ataaatccac attcagtgtc cgatcattcc acaattcttt
    26341 caagccttcc gatgtttgtt tatatccacc gatgataatg gcactgatat aaaaaagtgg
    26401 ggtgatggta agagagaatc ggtcgaagat aaaaccgaag atcataaata aaaggcagcc
    26461 gattgttgct gcaaaagctt ttttctgatt agtcatcgtt attagacctc cttgtcaaaa
    26521 caaggatagc ataataaaaa tgtaattgat aataataaca gctaaatgag aacgataatg
    26581 gttatcattt ggtgattaag cttgatgttg tttttattat ttataatgat tattattaaa
    26641 gaaaataaaa aaccagaaca tttctcaaat aagagaagtg ttctggtttt ttctacattg
    26701 caataaacca ataaccaatc aaaatgatac caaggataat acgataccag ccaaaagcgg
    26761 taaagtcgtt tttcttcaaa tagcttagta agaatttgat agctaagacg gaaaccacaa
    26821 aagatacgaa tgttcctaca agaagaacaa cagtttcttc cattttgaat gaattccctt
    26881 cagctaaata tttgacgatc ttcaagccgc ttgcgccaaa catgacagga atccctaaga
    26941 aaaatgaaaa ctctgtagcg acaaaacgcg aagcaccaat caagatagat cctaggatcg
    27001 ttgcgccaga acgggatgtt ccgggaacaa gtgccaagac ttggaataac ccaacaagaa
    27061 gagctgcttg gtacgtaaag tcattcagag aagtccaacg aggtgttttg tttttgttgc
    27121 gtttttcgac aataataaag gcaataccgt aaacgatcaa aacaatggca actggtaaga
    27181 acttgtggaa atttgcttct aaccaatcat ccaaaggcaa accaatgatg atggctggta
    27241 tgcaagcaac gatcacttta aaccaaagtg tccacgtatc ttttttttct accgttgatt
    27301 tacttggaga aaatgggttt aacttgtgaa aataaagaac gacgacagcc aagatcgccc
    27361 ctaattggat aacgacattg aacatcgaca taaattgtga actggcatcc agtttgatga
    27421 attcatctgc taggatcaaa tgaccagtac tactgatggg cagccattcc gttattcctt
    27481 caataatacc gagaatcact gctttgataa tatttgcaaa aaacataaat tccttccttc
    27541 ccaattaaga tacatcttat agtataccgt tattttttga gaaacccaat ctgtttttta
    27601 atattaagaa aaacttgaga aataaagcag ataagtacac gaagagaaaa aagaagacaa
    27661 agccctcgtt cagactttgc cttttatgat tctctggtaa aaattaaaat atccccatta
    27721 tagaaacgag tcgcgactat gtgttttccc gttatcgata taagacgtat cattgatgcc
    27781 gtggatagaa aatcgaccgt ttgcataagt gacctttgtt acgctgccat ttaaaagccc
    27841 agcacgaaca ggctgtttag gatcgatcag attaagcaaa aaggcgatcg tcaaaccatg
    27901 agatacgact aaaacatttc caccaccatt tttttcgcag tgatgggcga tatcttcaaa
    27961 tcctgtccat acacggtctc gcaatgtttc ataagtttcc gcccacccag cggtatctaa
    28021 gttgtataca gcattagcaa tttgttcaaa agaaacttct gtggtaaaca tttcatcata
    28081 tgttttaaaa ttcaatacac gagggatcac accccacatt tccatgtcat atccaccttc
    28141 aagagacccg aaacaccatt ctctaatcct tttttcaata aaataaggaa tttcttttcc
    28201 cttagaatgt tctcctagaa taatacgggc agtttcgatt gcacgaccac tgtcactaga
    28261 ataagcaaat gaaaagtcga cattttttaa cccgcgacct aaataatgaa tgccttcttg
    28321 tccttgcttt gtgaggggtg tatcacacca gccttgtact cgttcaattg tattgaacat
    28381 cgtttttccg tgtcgtacga tataaagtgt agtttctgtc attttattct cctcctaagt
    28441 cgttttaacg attgaaaaaa ggatttgtcc ttttttcctg ttcaatcgtc gttgcatcgc
    28501 catgtcctgg atagactgca aattcatcag gtaatacaaa cagctgggtc cgaatactat
    28561 gaagtaattg ttctaaattc cctgtataca gatcggttct accgatactt cctctaaaca
    28621 gggcgtcgcc agaaacgaca aaatcatcaa aaataaagct gacgcttcct atagaatgcc
    28681 ctggggtagg gacgacgcga aaagacatgt ttcctaaagt ataagttttc atttcaaatt
    28741 catattctgc aggcgagaca ataacatcag caatatcatc atgtctaccg agtccagaaa
    28801 gattcaaaat cggatcacct agccattttt gctctagtgg gctgacataa actgggattt
    28861 gataatacgc gcgtaaaggt tccactgcac cgatatgatc gtagtgagta tgggtaagca
    28921 aaatcgctac cggctgttgg tttgtttttt tgatttcttg tttgattcgt tccgcatctt
    28981 ctccagggtc aatgatcaaa agtgcttctt cgttgtagac caaataacag ttttcttcaa
    29041 tcattcctgt ttttaatctt tcgatatgca tgtgcattcc tccttgatag ccaaaacttc
    29101 tttcatccat tagtataacg cattcattgc taggaaacaa aaacaggtac ttatttctct
    29161 ttacttatct gaattatcag actatatgaa aagtatttgg atattctaaa gagaaggaaa
    29221 gtaagttttc atagcatttt aatatgtcct tgttacatta gattcatcca aaaatccaaa
    29281 ttctatatcc tatatccctg cctaatcagc agggtgtttt tttatgtttg gaaagagagc
    29341 tctttttgct aaactaggtt ttttcaggta atcttagaga gaaagaggtg gtggaaatga
    29401 tcaaacagtc aaaaatatct gtgcggcact tagtagaatt tattttgcga cgtggaagta
    29461 tcgacaatcg taaaaaaagt aaccatactg cattagaagg agcgagaatc caccgcaagc
    29521 tccaaaaaga tgcgggagaa aactatgaaa aagaagtctt tttaaaaaca acagttcaat
    29581 tagatgaata ttacctgatc gttgaaggcc gtgcagacgg tattttcaaa agagacggta
    29641 tttattatat agatgaaatc aaaacctccg aaccacgatt tgaagattta gaaccagaac
    29701 aagtggaact tttctttcat caagcaaggg tgtatgccta tatctatagt caagaacatg
    29761 atttacaaga aataaatctg caattgactt acttccagac ggcagaagaa gtgatcacga
    29821 gaaaggttga acatcagaca agagagcagt tagaaacttt ttttaaaaag ctgactgaag
    29881 attataaaga atggcttgtt tttcaagaaa actggcgcac tgtacgcaat gcctctttga
    29941 tggctttgaa attcccccat gatgagtata ggaaaggtca gcgtgaacta gcggtagctg
    30001 cttataaaac gatccgtacg aaacagaaat tgtttgtaga agcacctacc ggaacaggaa
    30061 aaacaatctc tacgttattc cccgctttga aagctgtagg ggaagaagag ggagaaaaga
    30121 tcttttatct aacggctaaa acaataacta gacaagtagc tgaagatgct atgacagcat
    30181 taaaagatac aggagctgaa gtaaaaagtg tcactttgac ggcgaaagac aagatctgct
    30241 ttttagatga gacgacctgt aatccagatc agtgcccata tgccaatggt tattacaatc
    30301 ggattaatga aggtttatgg gatcttttga atcatgagaa tcagatcact cgtgaagtca
    30361 tcgaaactta tgcaagaaag catacacttt gtcctttcga actatcatta gatgtcagta
    30421 tctggtgtga cgtaatcatt ggggattaca attatttatt tgatccaact gtttatttaa
    30481 gacgtttttt tgaagatgaa aaaaacgagg actatctgtt tttgattgac gaagcacata
    30541 atttggtgaa tcgttcccga gagatgtatt cggcagaact atcttatgaa aaaacgaaac
    30601 gaagtaagga agctatacca aaggaattca aaaaacttca tcgccgtttc aacaaactat
    30661 taaaagaatt tgattcgatc cgagaaatag caaaagaaga tcattgggat tatcatcatc
    30721 aaaaagcacc ggctgaatct ttagtaaagg caggttatca attaagcgaa aaaattaaag
    30781 aatggctggc agaatttcct gagcacccat cacaagaaca attgcttcct tattattttg
    30841 atttgcttca ttttttgaag gtcagtgaat tttatgatga tcactacgaa acaacagtcg
    30901 aaaaaacgta tcgcgatctg atagtaaaag agttttgtat tgatccaagc ttgtttcttg
    30961 agcagtcgtt ggataaagga aaaagcagta ttcttttttc tgctagtttt tcaccactct
    31021 cttattatca ggaaacactg gggggacaaa aaagtctggc ttacaaactg ccgagtcctt
    31081 tcccagaagg aaaccagcaa gttttcatcg caaattacct agagacgact tatcgaaaaa
    31141 gggaacacag tctggcaaaa ttagtagaga cgattcatgc ttttgctgat ggaaaggtcg
    31201 ggaattattt cgttttcttt ccttcttatc aatatttaga tcttgcggtg gaagcattca
    31261 gacagcaata tcccgacagc aatgtgctaa tccaagaaac ggacatgaat gaggaagaac
    31321 gcgaacgatt tttaacaaaa tttcaaacaa atcccgctga aacattgatt ggattttgcg
    31381 tacttggcgg gattttttca gaaggaatcg atttgagagg ggaccgattg atcggcagta
    31441 tgatcgtagg tgtaggtttg ccgcagatga atcatgagca ggaactgatc aaatcatatt
    31501 atgatgagaa ggaacacctt ggatttgctt acgcttatca gcttcctgga atgaacaaag
    31561 tcttgcaagc tggtggtcga gtgattcgtg gcatggcgga tcaaggaatc gttgtattgg
    31621 cagatcaacg attttctagt ttttcttatc gacgtttatt cccacaacat tggcaaacag
    31681 caagagaagt acgaagtgtt ggggaattgg aagaaggaat acagcgtttt tggttatcaa
    31741 aagaagcagt aaatcaaaaa aacgaggaat gacagacgtc agcctcgttt tttgattaac
    31801 gatagttagt gaattgcaac tcgattggta agtcaagttt acgtaataaa gcaatcacag
    31861 cttgtagatc atcacggttc ttacctgtca ctctaatctg atcttcttgg atttgtgctt
    31921 ttactttcag tttttcattt ttgatagctg tagtgatttt cttcgcgttt tcacgatcga
    31981 ttccgttgac caaatcggca gtttgtcgag ctttgccacc tagagcgtgt tctgattcgg
    32041 aaaaatggat atttttgatt ggcacatttc ttttgatcag cttaccaaat aatacatctt
    32101 tgatctgttc gattttgaaa tcatcatcgc tcaatacaac tagattgctg ttctctaatt
    32161 tgatttctac tgtagaatcc ttgaagtcaa agcgattgga caattctttt gttgtttgct
    32221 gaatcgcatt ttttacttct tctgtattga cttctgatac aatgtcgaag ctagcatctt
    32281 ttgccatagg aaccctcctg atatacatat aaatatttac catacatcta tcttatcata
    32341 ggattagggc agattcttac ctgtttgcta aaattcttct catttgagtt cgaagtattt
    32401 tattttaata gataaactgt tattcttaca tttggaatac caaagtaggg gagaggtgct
    32461 tgtgagtaaa attatgactg cgtatcataa aaatgaaaga ttaaagaaaa tagtggttat
    32521 tttgataacc ggattgaccg ctgcggtcgc tttaaattat tttttgattc cagccaatgt
    32581 cttttctgca gggatgaatg ggatcgctca aatcatttcc tcgcttttat atgaatggtt
    32641 tcatatcaag atagacacag gaacatttat ttttcttttg aatatccctg tgtttgtatt
    32701 agggtttctc aaagtaggaa aacgagcaac gatccttagt tttgtgaatg ttgtatgtgt
    32761 atcggtgatg acgatgctgc ttccagtcgg acagatcact gataatattt tgatgaatgc
    32821 cctagtaggc ggtgttttat taggtcttgg cgtaggatta tctttaaaaa tgggctttac
    32881 aactggtggg atggatatca tctctcttgt actttcacaa acaactggaa aaacagtcgg
    32941 aaactacatg ctgattctaa atggtatcat tgtagtcatc gcaggcttct tctttaactg
    33001 ggaaagtgcc ttgtatacga ttatttccat ttatgcaatg actcaagtaa ttgatgccat
    33061 ccacacgagt catcaaaaag taacagcaat gatcgttacc gtcaagccag aagaggtaac
    33121 agaagaacta gcaaaacgga tggtacgtgg aatgacatta ctgccgtctg tcggtggcta
    33181 ttcaaaaaaa caagggaaca tgatcatgat ggtcatcacc cgctacgaaa tgtatgatct
    33241 tgatcagatc gttcatgaaa tcgacgacga tgcatttatc aatattttac caactcattc
    33301 agtatttggc cggtttgcaa atgaaaacga acaaaaaatg tatcgaacaa ctggcgtatt
    33361 gccagaaata aaaaatcgtt cacaaaaaaa atgaggaatc aggtttattt tagaataaat
    33421 ttaatctatt acatctaata agaacatttt tataaattta aacgagaatc ttacttctaa
    33481 attctcattt acaagcagct aaaaaaagag tatgataaat aaaaatcgtg ttaaggagtg
    33541 tcgtacgatg agcgaagcaa accaagaacg gtctggacaa gaaatagaac taatcaaaga
    33601 acgtattctt gctgccttgg aaatggtgat tgatcctgaa ttaggaatcg acatcgtcaa
    33661 tttaggcttg atttatgata tagaattcaa tcctgaaaat ggagaaacag tcattaagat
    33721 gactctaaca actatgggct gtccattagc agatatttta acagaatcta tccatgacgc
    33781 attaaaagaa gtgccagaag taacgaaagc agaagtcaaa ctagtctggt atccagcatg
    33841 gacgacagat aagatgagcc gttatgcacg gattgcgttg gggatacgat agaaatcttg
    33901 gctgactagc tatgagaagc aaataggaaa aacaaaaaag ccttttatcc tttctagaag
    33961 aaaagagata aaaggtttct ttgtttataa aattttgtct gctatattat aagttaatta
    34021 aataaatgtg ttctattgat aaaggacatt caaatccctt tccttttgga atccttcttt
    34081 tttacatcag tttgattttc gtatagtatt ctttctcatc ttcacgataa atcgcagccc
    34141 catttttttg ttgtgcttta agactgccaa gatttttttt atttacagac aaaagtgcct
    34201 catcaatgcc gatttttttt gcctcttgta gtactaatgc taatccttgt gtggcatatc
    34261 cttttttacg ataattaggg gagatgccgt aaccaatatg gccagcgccg tttcttaatg
    34321 tttcgttcag tttatggcgc aaattaaaaa ttcctacata ctcatgttca tcactttcta
    34381 aaataaagac ggttgcggga acgtaactct caggaagatc gattcctaaa gacgtttttt
    34441 cgcatttttt tacataatct aaaaattcag caaatgtata gccataagca ttgttgataa
    34501 atccactctc atttggtcca aagctagttt gtagctgata agctttcttt aaatctgctt
    34561 cccatagttt gatcagtttc atcgtattca cctctttaag taaccttatt catttgtttt
    34621 ctactatagc actattgttg taaatagcca tgcctatttt tatttattgt gtcatatcgt
    34681 attaagaatc atcgaacaat acaaatatga attacaataa aaaaattatt tgaagttcgt
    34741 tcattcgtta cttatgatct ctagaagaaa gaaaacgtga attacttcag cgtctagaat
    34801 tccagaagga tagtaatcaa cagaagaaat aatcaatatc caaatgaaag aagatagctg
    34861 aatcgagaaa aagaggaaaa aagaaatgaa gaaaaaaata aaatagttac taatcccaga
    34921 gtcgttcgtt taacattgaa atcgatcata tggataaaac agattttttc accaaccctc
    34981 aatagatgat ggagttgctc ggatgaatta tttgtgtaga tggcataagg gagggcgtac
    35041 agaaatgaaa aagaacaaaa atatgttctt gacaaaaaac acatgtaatc ctaaactaaa
    35101 agatgtgatg cggctatggc ggaattggca gacgcgctag cttcaggcgc tagtgagggt
    35161 gacctcgtgg aagttcgagt cttcttagcc gcataatttg tttttttata aagagaaggt
    35221 acacctggag cgatgagaaa gaaaataaca tattatctaa aagaatatag gaaaaatttt
    35281 aaacaacgga accagattca aataaatcaa aaaaaatttg tgtgaaggtg ggacagttta
    35341 aaacctaata gattgattca aatagtagat ttatagattc tgatacaaga aatactggca
    35401 aacgatagtt aaggaataaa gactaaactt aaactcaaaa agaggtcatg cgaaagaata
    35461 aatcgcatga cctcttacta tattttgaat gtgtttaaac gttcaaataa tgaatcttag
    35521 taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta
    35581 acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa
    35641 gaatctaatt ggtaacggcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat
    35701 tctttttgtg cgatccattc ttttgcagag cttgtacttg ctggagtagc agcagtttct
    35761 gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtgtttc cgtttgaacc
    35821 gtttcttgta caacaggttg ttcagcttga acaggttcag cttggactgg tgcttcttga
    35881 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
    35941 tgtcctgagt agatcaggtt aatatcagaa attgaatttg attctgcaat ggcattgatc
    36001 aaggagttat caccaacgta tttttgagaa atcgttgata gagtatcacc tgattgaacg
    36061 gtataagctt cgtccgcatg agcagtcgtt cccatgaaga atgcagcgcc ggcagccaaa
    36121 gttgttccaa aaagtaaagt tttaagtgat gtcatgaaaa atctccttta ttcgttaaaa
    36181 agttttattt gttgtgttca acgaggaata ctttaacacg tgaaaatatt tcataggtaa
    36241 aagaagcatg acaatttatg acaaatccac gttttttgta acagaatgat gttcgctcga
    36301 tgtgaaaaat gatgaggaat ttcacataat gcagaaataa tgggattaga acaggtgttc
    36361 tgatgagaaa agaaatgtat aaataacttg aaagaaattg cttttgttca tcattttatt
    36421 tacatattgc gtatattttt tgtatttttg cttgagatgt tacaataaaa gaaaaaggag
    36481 gcggttccaa tgagtaaaag ataccagaac atcatggtag cagttgacgg atcaagacaa
    36541 tcgatcaaag cgttcgctga agcccttgac ttagcaaagg acaatgaagc ccacttattt
    36601 atcgtgtcaa tcatcaacaa agttgagctg acccatagtg cctatgcctt ttcaaaaatc
    36661 tatgcagatg aaaaacaaag aacagaagta gccatgctga aaaaaatcaa tgatgccaaa
    36721 gagtacggca ttgctgagat ccacgcaata gttgaaactg gagatccaag aaatttgatt
    36781 gccaatgtca tccctggaca agaaaatatt gatttgatcg ttatgggagc gacaggaaaa
    36841 ggtgctatac aacaagcatt agttggatcg accgcttctt atgtcgtatc acatgcccct
    36901 tgcagtgtgt tggtcgtgaa gtaatgagat aagggaggaa tagaaaatgg ttcgtcctca
    36961 agaagtccgt gcgccaaaag aaaaaataga aattttagca attattgaag acggcacaca
    37021 aacaaagaag ggatactcga tcgctttagt aaagtggaaa ggaaaaaaag gtgttgctct
    37081 gagatgggat ggagataacc aacaagataa aggattccct gtcacagcta acggttatca
    37141 tccagcgtgg tttgttctgc ctgataaatt tatggaatta tacagctatg attatgcaag
    37201 agccgcagca tcaatcaaag cattagataa attaacagaa gagcagaatg aaatggagga
    37261 aaaataaaaa agacgaacca atcagccttt agaaaactga ttagttcgag gagtaaaaat
    37321 gaaaaaaggt tatttgggat gaaactagaa taatgcgtat aacttaaata aaacttaaag
    37381 caataaaatg gcaagagggt gaggcaaatg gaagcaggaa aagtattaca ggaagtaaaa
    37441 gcactatgtg cagataaaaa atatgaagaa gcgaaaagcc tgatcgaaaa gaacaaagaa
    37501 aatttggaag aaaagtaccc gatcgcacaa gaatttatag aattaaaaca aggaacgatc
    37561 cttcaccgat tcaaacattt ttttggtata acagaataga aaaaacgatt aaagatcggc
    37621 agaaatttgc tgcatcttta atcgttttct ttttaaacat gcgagggatt caattctgat
    37681 tcttatataa aaaatagatc caaataataa gaaaaattac tgaaagcgtc aattcgatga
    37741 tgtgtgttat taatacagac gttgtttccc aatgcctaaa agaaaaaatt tgataagtaa
    37801 aatatacagc aggtaaagag gccagtaaaa ctgaaaactt cttttttgtc cattttatgt
    37861 aatagaaaag aatgcttatc ataataaaaa aaggaatata aaaaacaaat tgaccgatcg
    37921 cgatcatact attgcctccg ataaataaag tccatgatac aaaataacac tttgtgcgaa
    37981 atggcgcaag cagttttgtg gatttatccg gtaaaatctg tatcgatttt gaaattagag
    38041 gagaaacttg ctataatcaa agtgaaagaa ggagtgggat tttcatggat catgtagtgt
    38101 tagaaatcga tttgcatttg atcaataata ttcgggtgat ttactatgtt gtaagtaaca
    38161 gtgtcgaaca acgtgtactg acgaataaaa taaatggtat tttagcaaag aaagacgttc
    38221 accgattcaa taatggtgaa ggaagttatt atagtatccc agtagaaaaa attatttata
    38281 cgacagtcaa agtcagagaa gatctagata ccaaaaaagc atatgaacct atttttacaa
    38341 cttattgatt gacagcagat ggaggaaaaa aatgaatcca tatgaacaac ttattcaaga
    38401 tttgatcgat ggaaaaatcg aaaagattga tgtaaaaaga gaagaattaa tggcttttcg
    38461 agaagcgtgg ctgaagttgg agaatcgtaa atatgtggtg ggaaaagcct cactgaacgg
    38521 caatgtgact tatcattacg atccgacacg tttattttag tatgaagtat tcagaaaaga
    38581 accaaaggag gacgatgcag cagcattgtc ctcctttgat tttacaaaag tgaaagtttt
    38641 acttatttga atagattttt aaatttgttt ttgacactgt cgatatttac atcttttaaa
    38701 agattttggg ctttctcttt ataaggtcct aattgttctt tatgttcttc gataaatcgt
    38761 tttgcgccat cgaaattgcc gttcttaatc atttccttga ctttttgaaa taattcttct
    38821 tgattcattt tgaacacatc ctttgctatc aacatgattt tttacatgga caaaacgaga
    38881 aaaactgatt tatccacacg tcaataatat caaataacga tcatttttac taaaaaaaca
    38941 ctcgattctc gcttctaaag taggagaaaa atctccaact tgggacggat ggaatttctt
    39001 taactgaccc atatactgaa tatataacaa ggaggtagaa agatgaaagt gattaaatat
    39061 ttgattggac tggcggcgat tagtggtatt ttgctatttg gactgaaata ttatactgaa
    39121 ggaagttatg gagaaatccc tggtatactt gatcagctaa atccattagt tgaaaaagga
    39181 gaggtctatg taaagacaca aaaaccagag gaagtaaatg aatatggtac agcaaggtat
    39241 gtgcagaaag ctgctgatgc caatgggaaa gaacgaacgg ttgaatttaa tggtctttct
    39301 gtgttaaaag aaaatcatta tttgaaaatt agcaataaag gagcacacat agaaacgtat
    39361 gaggaagttc ctagagaaaa agttccggag aaagcattag caattattgg gtaaatcagt
    39421 ttttgtatta agaatacgcc cgattttatt attcataatt taattatgta aactaaatac
    39481 aagtagtaaa aataatacaa taatccccct gcaaaaagat tgttctttta cagggggttc
    39541 attttttcag caagcacatc aaattgggat ggcagggttc gaacctacaa ttgcttgatt
    39601 cagagtcaag tgccttacca gtttggctac atcccattta ttcaacaaaa taatcatagc
    39661 actttcctaa aaaaacgcaa gttatttttc ttgatcggat agttgttgct ttactcgtaa
    39721 aaggcggcgt actagtttct tttctaatcc agcaggaagg gcaatcagtt ctgctactga
    39781 caagacggtg tccaataact gatattcttt gacaggttca ttcataatat gatagatgtt
    39841 tgctaaacgt tcaatatatt caaagcgagt atactctgtt cgagcaatta gaaatttcaa
    39901 taaatcggtg gctttctcgt actcttgatc tgcgtaataa acaagagatt gacgatacaa
    39961 tttgatttcc agttcattga gggtagcagg tctgcgacgc tcgttccact cattaataga
    40021 gactttacct tgaacatttc ccttgcagtc acggacaaaa ataatcggaa tttcatttgg
    40081 atccattttt ttatgtgtga atttttccat tatctacgct cccttcaaag gtttggtatc
    40141 ttgagtataa gggatttttg tttgaattac tagcttttct ccaaagttag catctgtcca
    40201 cccattgcag tgaaaatcgc cgaaaaatcg ttgatcggta ctgctcgatt ttttacacca
    40261 tagggatcgt tgacataaat attttctttg tcatatccag tgactacaca agcatgaaca
    40321 agaggagtga ctcttacttc tccattattt gtggtccaag tcataaaatc agtttcatct
    40381 gggacttcaa aattgatagt cacagaagtc cagacaggat ttcctttaga tagtgctttt
    40441 ttgatatcat cgagttgagc atcggttttt ggaaaagtgt gataatccgt ttggacgatt
    40501 tgctgagcaa gggaggcaac tgggggaaca tagacgacca tagcccagtc tccgccactg
    40561 atatcaccaa caaatccttc attaggatcg ccatgagtcc catctgcatt gaaaacaggg
    40621 acgtaatcca attgctcagc taactggttt ttagttgttt cataaccata aaaagataaa
    40681 agcatagaaa gcgaagtgac ttcacagcca ttttctaaag aaggagaatc aaactggctc
    40741 tctaatggaa catctagtaa aacttctgct ttctcttttg tctttttcgt tttttgtgtt
    40801 gaactttttt gtgcagtgtt cattgtatgt tctggagaat ggatatgtag gtaataactt
    40861 gtaccgctta ataaaagagc agctgtgccc cctagaaaca atagtttttt gaacgttttt
    40921 ttcttcattt gaaatctccc ttttcagctg tttatctttc ctattataaa tgtattttta
    40981 ttggaaatgg aagtattata ttttaatatg taggaaataa ttagttttta aggaatatgt
    41041 tttaattttt tgaaaaaaat tcttctaaat acaacagctg ttttaattgt gaaaacgaaa
    41101 acagtacgct ataatgcctg taaggttgat ttttaactgt aaataaaaat caatgagtta
    41161 aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt aggaggattt
    41221 tgatgacgga agaaatgatt aatttaggag aacaatatgc atgcaaacca attggtttta
    41281 caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg gtcaaagttg
    41341 cccaatgtgc tgcagaggac caagaattat tagatgaaaa agcaagcatg gttgttgcaa
    41401 aatatgatac atttgaataa agaaaacaat tggacgtgcg gcgtgctgca agtccaattt
    41461 ctttgagaag gtaaatcagt attcttttcc tattttctgg agaagaaaaa gcgaggcgag
    41521 ttgtcatggt aacattcttt acggttctat tgtttttatt cgttgttgtg atgtttttat
    41581 ttctttggct ggttcgaaaa gaaattattt atcggactgt gcgaaatcga tgggtctact
    41641 tggtgatacc cttcttagtg tcactagtca tttggtatac actcatcagt catcctacag
    41701 cagatgaatt ggcaaaaggg atcttgtcag caatgatttt tattagtttt ttgttggaca
    41761 gtagaggaat cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa ggtgttcctt
    41821 tttctgagat caataaaatt gtattatatc agccaaaagg ttctaaaatc gtaaaaatga
    41881 attttttccg aaacggatgg cgaggaccga tgctgaaatt ttctgcatca ttagaagaat
    41941 tagttccatt tttgtcccaa cgcttaaacg atgaggcaga gattgatatt atgattgatc
    42001 cagaataaaa acagaggttg cgacaagatg tttgtcacaa cctctgtttt ttatttcaaa
    42061 taaaattatc caaatacgcg gaagcgatcg gcatcgttca tgtattcttt ttccccagcc
    42121 ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt tccagtttga agggatattc
    42181 cattcttttg caactgcttc gtcaataact gggttgtaat gttgcaagtt tgcgcccaat
    42241 cctttatcaa ctaatgctac ccaagtatta gctgttgcga ttccgtttga ttgttctgac
    42301 caatctggga agttgtctgc atataattca aattgttctt gtagaccttt taccacgtca
    42361 gtatctttga agaataatac tgtaccataa ccatttttga aaccagctaa tttgttttgt
    42421 gtatttggga aagcttctgc tggagtcaat gggcgaagtg cttcttcagt gatttcccaa
    42481 agtttttcat gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc attgaaagca
    42541 gttgggcttt ctttaattgc ttctttgatc aaagttgtta attcttcatt agaaagtgaa
    42601 acattacgtc ctagatgata gattgaacgg cggtttttca aagtgtcagt aaaagttgtc
    42661 ataataaaat ttctcctttg atttgcgttt tgcatttttt taattgatta cgaaatgaag
    42721 tatagcacct tacaaaaagt aagtaaacag atatgctcac aaaaaataag cgaagttcaa
    42781 aaaacaattt taataagaaa aatcgttatg taaggatttt cttagttatc gctttcattg
    42841 ttgtgtttta ggaatttttg cttatttgaa aaaaacagct gatatataat tattaaatct
    42901 ctatcttctt ttttttatga tattgccatt aaaaattact tgcttttata gaaaattagt
    42961 ttatatttct taaataagtt ttttagttaa atttttcggt aaagatgtat aatattttct
    43021 gaaaatttaa agaattgggg tatagattat ggaaaaacag caagacaagg gcttcttcgg
    43081 gcagccgaag ggattgtcaa ctttgttctt cactgagatg tgggaacgat ttagttatta
    43141 cggtatgcgt gcattactta tctattatat gtacgataca gttgccaacg ggggacttgg
    43201 attaccacga gcaacagcat tagctatcat gtcgatctac ggatctttga tctacatgtc
    43261 tagtatcgtc ggcggatggg tatctgaccg agtgttagga gcaaaacgca ctgtattctt
    43321 cggtggagtt ttcattatgc ttggccacat cgtcttggct ttaccatttg gtgtaagtac
    43381 attgtttgtt tcgatggcat tgattattat cggaaccgcc tttttgaaac caaatgtttc
    43441 tggcatggtc ggacatcttt attcaaagac tgacttgcga agagatgcag gattttcgat
    43501 tttttacatg ggtgtcaact taggggcatt attagcacca ctaatcgttg gtacaatcgg
    43561 tcaagaatat aactatcatt tagggttctc tttagctgct attgggatgt tctttggatt
    43621 attacaatac atcatccaag gacgcaagac attagacgga gtgggtacaa caccgccgaa
    43681 tcctttgaca actgaagaac gtaaaggatt catccgtaac ttgatcatcg cacttgttgt
    43741 tatcgctgca gtattcggcg gggcacaact aactggtcat ctgacaattg acttttttgt
    43801 caatacaatc agtgtattgg ggattatctt accgttgtat tactttatca aaatgctgac
    43861 ttcaaaagat gtaactgctg aagaaaaacc aagagtatgg gcttatatcc cattattcct
    43921 tgcagcaatc attttctggt cattacaaga acaaggttca tctatcttag ctttatttgc
    43981 tagtgaacgt acacaatcta cgctatttgg tgtaacgatc caagcaagct ggtatcaatc
    44041 attgaatcca attttcatct ttattctgac accagtattc gtaacgatgt ggacgaaact
    44101 tggtgaacgt caaccatcta cagtagtgaa attctctctt ggacttttat ttgcaggatt
    44161 atcgtacttg ctattgatgt tgccgggtat gctttatggt acaagtggaa aagtaagtcc
    44221 attgtggttg ttgggaagtt tcttcatcgt ggttattgct gaattgtgtc tttcaccagt
    44281 tggtttgtct gtgacaacaa aattagcacc acgagcattt gaatcacaaa caatcgctat
    44341 ttggttctta gctgatgctt cttcacaagc aatcaatgca caaattgcac gcttctacac
    44401 accgggaaca gaatctgctt acttcggtat tatcggtgca gtagcaatcg tcggtggtat
    44461 cttgctattt actgtgaaag aaccaatcaa aaaactaatg ggctctattc attaatcaat
    44521 gaacataaaa acaggaattg cttatttgca gcagttcc
//
LOCUS       DAFBQW010000018        40821 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFBQW010000018 DAFBQW010000000
VERSION     DAFBQW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.018"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            10..588
                     /locus_tag="LK889_001239"
     CDS             10..588
                     /locus_tag="LK889_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095485.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="LK889_001240"
     tRNA            complement(828..911)
                     /locus_tag="LK889_001240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            966..1145
                     /locus_tag="LK889_001241"
     CDS             966..1145
                     /locus_tag="LK889_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095486.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1294..2511
                     /locus_tag="LK889_001242"
     CDS             1294..2511
                     /locus_tag="LK889_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5095487.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="LK889_001243"
     CDS             2695..3531
                     /locus_tag="LK889_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5095488.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     NEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="LK889_001244"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="LK889_001244"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK5095489.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4976..6034
                     /locus_tag="LK889_001245"
     CDS             4976..6034
                     /locus_tag="LK889_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBK5095490.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6038..7072
                     /locus_tag="LK889_001246"
     CDS             6038..7072
                     /locus_tag="LK889_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBK5095491.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7174..7890)
                     /locus_tag="LK889_001247"
     CDS             complement(7174..7890)
                     /locus_tag="LK889_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5095492.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7859..8644)
                     /locus_tag="LK889_001248"
     CDS             complement(7859..8644)
                     /locus_tag="LK889_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBK5095493.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8635..9384)
                     /locus_tag="LK889_001249"
     CDS             complement(8635..9384)
                     /locus_tag="LK889_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK5095494.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9682..10206
                     /locus_tag="LK889_001250"
     CDS             9682..10206
                     /locus_tag="LK889_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK5095495.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10222..11721
                     /locus_tag="LK889_001251"
     CDS             10222..11721
                     /locus_tag="LK889_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK5095496.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11809..12411)
                     /locus_tag="LK889_001252"
     CDS             complement(11809..12411)
                     /locus_tag="LK889_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5095497.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12515..13471
                     /locus_tag="LK889_001253"
     CDS             12515..13471
                     /locus_tag="LK889_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBK5095498.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13776..14732)
                     /locus_tag="LK889_001254"
     CDS             complement(13776..14732)
                     /locus_tag="LK889_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5095499.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFVSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14906..15466
                     /locus_tag="LK889_001255"
     CDS             14906..15466
                     /locus_tag="LK889_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095500.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15459..16283
                     /locus_tag="LK889_001256"
     CDS             15459..16283
                     /locus_tag="LK889_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK5095501.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16293..16910
                     /locus_tag="LK889_001257"
     CDS             16293..16910
                     /locus_tag="LK889_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK5095502.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="LK889_001258"
     CDS             16928..17611
                     /locus_tag="LK889_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK5095503.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="LK889_001259"
     CDS             18294..19577
                     /locus_tag="LK889_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5095504.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="LK889_001260"
     CDS             19593..20480
                     /locus_tag="LK889_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5095505.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="LK889_001261"
     CDS             20497..21333
                     /locus_tag="LK889_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5095506.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="LK889_001262"
     CDS             21336..21959
                     /locus_tag="LK889_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095507.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="LK889_001263"
     CDS             21956..23248
                     /locus_tag="LK889_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5095508.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="LK889_001264"
     CDS             23820..24401
                     /locus_tag="LK889_001264"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK5095509.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="LK889_001265"
     CDS             24404..25711
                     /locus_tag="LK889_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBK5095510.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="LK889_001266"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="LK889_001266"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK5095511.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26608..27732
                     /gene="purK"
                     /locus_tag="LK889_001267"
     CDS             26608..27732
                     /gene="purK"
                     /locus_tag="LK889_001267"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK5095512.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="LK889_001268"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="LK889_001268"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK5095513.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="LK889_001269"
     CDS             complement(29220..29597)
                     /locus_tag="LK889_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095514.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="LK889_001270"
     CDS             29853..30581
                     /locus_tag="LK889_001270"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK5095515.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="LK889_001271"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="LK889_001271"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBK5095516.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="LK889_001272"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="LK889_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBK5095517.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="LK889_001273"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="LK889_001273"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBK5095518.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="LK889_001274"
     CDS             33716..35155
                     /locus_tag="LK889_001274"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK5095519.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35178..36230
                     /locus_tag="LK889_001275"
     CDS             35178..36230
                     /locus_tag="LK889_001275"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK5095520.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="LK889_001276"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="LK889_001276"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK5095521.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="LK889_001277"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="LK889_001277"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK5095522.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="LK889_001278"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="LK889_001278"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK5095523.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="LK889_001279"
     CDS             39749..40663
                     /locus_tag="LK889_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5095524.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT        13056 a         6975 c         8972 g        11818 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaatcgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcaa cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     4981 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5041 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5101 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5161 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5221 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5281 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5341 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5401 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5461 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5521 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     5581 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     5641 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     5701 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     5761 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     5821 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     5881 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     5941 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6001 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6061 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6121 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6181 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6241 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6301 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6361 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6421 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6481 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6541 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     6601 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     6661 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     6721 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     6781 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     6841 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     6901 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     6961 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7021 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7081 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7141 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7201 cccattcttt gtcaattcat aacaggttag cggccggccg atttccaaat atttgattct
     7261 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7321 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7381 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7441 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7501 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7561 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     7621 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     7681 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     7741 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     7801 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     7861 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     7921 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     7981 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8041 gcgtaaatat tttttctgag atttcttctg atatcccgac accgtcatcg cagacggtca
     8101 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8161 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8221 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8281 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8341 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8401 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8461 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8521 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     8581 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     8641 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     8701 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     8761 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     8821 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     8881 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     8941 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9001 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9061 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9121 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9181 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9241 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9301 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9361 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9421 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9481 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9541 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
     9601 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
     9661 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
     9721 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
     9781 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
     9841 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
     9901 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
     9961 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10021 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10081 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10141 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10201 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10261 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10321 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10381 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10441 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10501 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10561 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    10621 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    10681 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    10741 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    10801 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    10861 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    10921 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    10981 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11041 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11101 atcgtagaac gtcaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11161 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11221 gcacaagctc aaaaagtcaa agaagtgact gaagccgaag cagaacgatt ccgtgtggaa
    11281 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11341 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11401 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11461 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11521 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    11581 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    11641 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    11701 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    11761 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    11821 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    11881 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    11941 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12001 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12061 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12121 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12181 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12241 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12301 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12361 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg ccaaattgca tagagattct
    12421 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12481 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12541 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    12601 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    12661 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    12721 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    12781 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    12841 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    12901 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    12961 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13021 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13081 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13141 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13201 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13261 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13321 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13381 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13441 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13501 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13561 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    13621 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    13681 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    13741 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    13801 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    13861 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    13921 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    13981 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14041 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14101 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14161 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14221 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgacgaaa
    14281 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14341 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14401 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14461 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14521 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    14581 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    14641 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    14701 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    14761 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    14821 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    14881 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    14941 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15001 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15061 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15121 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15181 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15241 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15301 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15361 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15421 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15481 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15541 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    15601 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    15661 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    15721 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    15781 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    15841 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa a
//
LOCUS       DAFBQW010000019        39855 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFBQW010000019 DAFBQW010000000
VERSION     DAFBQW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.019"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(28..726)
                     /locus_tag="LK889_001280"
     CDS             complement(28..726)
                     /locus_tag="LK889_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBK5095525.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="LK889_001281"
     CDS             complement(719..2071)
                     /locus_tag="LK889_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBK5095526.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="LK889_001282"
     CDS             complement(2294..3694)
                     /locus_tag="LK889_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBK5095527.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="LK889_001283"
     CDS             3855..4592
                     /locus_tag="LK889_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBK5095528.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="LK889_001284"
     CDS             4717..5196
                     /locus_tag="LK889_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBK5095529.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="LK889_001285"
     CDS             5193..5960
                     /locus_tag="LK889_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095530.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="LK889_001286"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="LK889_001286"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5095531.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="LK889_001287"
     CDS             complement(6863..7465)
                     /locus_tag="LK889_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5095532.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="LK889_001288"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="LK889_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="LK889_001289"
     CDS             8146..8730
                     /locus_tag="LK889_001289"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK5095533.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="LK889_001290"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="LK889_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK5095534.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="LK889_001291"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="LK889_001291"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK5095535.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="LK889_001292"
     CDS             10631..10810
                     /locus_tag="LK889_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095536.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="LK889_001293"
     CDS             10832..11857
                     /locus_tag="LK889_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK5095537.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="LK889_001294"
     CDS             12032..13276
                     /locus_tag="LK889_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK5095538.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="LK889_001295"
     CDS             13429..14376
                     /locus_tag="LK889_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5095539.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="LK889_001296"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="LK889_001296"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK5095540.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPGGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="LK889_001297"
     CDS             complement(15278..15520)
                     /locus_tag="LK889_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095541.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="LK889_001298"
     tRNA            15641..15713
                     /locus_tag="LK889_001298"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="LK889_001299"
     CDS             complement(15832..16470)
                     /locus_tag="LK889_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5095542.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="LK889_001300"
     CDS             complement(16593..17225)
                     /locus_tag="LK889_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK5095543.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="LK889_001301"
     CDS             17576..17875
                     /locus_tag="LK889_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK5095544.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18370..20535
                     /gene="acm"
                     /locus_tag="LK889_001302"
     CDS             18370..20535
                     /gene="acm"
                     /locus_tag="LK889_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBK5095545.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20807..21292)
                     /locus_tag="LK889_001303"
     CDS             complement(20807..21292)
                     /locus_tag="LK889_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBK5095546.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21382..21727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21772..22413
                     /locus_tag="LK889_001304"
     CDS             21772..22413
                     /locus_tag="LK889_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5095547.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22432..23067
                     /locus_tag="LK889_001305"
     CDS             22432..23067
                     /locus_tag="LK889_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5095548.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23085..23900
                     /locus_tag="LK889_001306"
     CDS             23085..23900
                     /locus_tag="LK889_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5095549.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24219..25655
                     /locus_tag="LK889_001307"
     CDS             24219..25655
                     /locus_tag="LK889_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5095550.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25844..28291
                     /locus_tag="LK889_001308"
     CDS             25844..28291
                     /locus_tag="LK889_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK5095551.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28521..29588
                     /gene="rlmN"
                     /locus_tag="LK889_001309"
     CDS             28521..29588
                     /gene="rlmN"
                     /locus_tag="LK889_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK5095552.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29781..30884)
                     /locus_tag="LK889_001310"
     CDS             complement(29781..30884)
                     /locus_tag="LK889_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5095553.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31437..31985
                     /gene="aac(6')"
                     /locus_tag="LK889_001311"
     CDS             31437..31985
                     /gene="aac(6')"
                     /locus_tag="LK889_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK5095554.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32531..34192
                     /locus_tag="LK889_001312"
     CDS             32531..34192
                     /locus_tag="LK889_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5095555.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34262..34609)
                     /locus_tag="LK889_001313"
     CDS             complement(34262..34609)
                     /locus_tag="LK889_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBK5095556.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34693..35634
                     /locus_tag="LK889_001314"
     CDS             34693..35634
                     /locus_tag="LK889_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5095557.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35635..36678
                     /locus_tag="LK889_001315"
     CDS             35635..36678
                     /locus_tag="LK889_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5095558.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36684..37754
                     /locus_tag="LK889_001316"
     CDS             36684..37754
                     /locus_tag="LK889_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095559.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37744..38697
                     /locus_tag="LK889_001317"
     CDS             37744..38697
                     /locus_tag="LK889_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5095560.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38709..39719
                     /locus_tag="LK889_001318"
     CDS             38709..39719
                     /locus_tag="LK889_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5095561.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
BASE COUNT        13599 a         6864 c         8124 g        11268 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgggggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa
    18181 taatttacat taggaaaaac cttgtaaaaa cagttttttg gcatatttat tttttataaa
    18241 tatttgtaaa ataactatca attttttagt taaaagattg gaaatgagtg ggggaggaat
    18301 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
    18361 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
    18421 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
    18481 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
    18541 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
    18601 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
    18661 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
    18721 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
    18781 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
    18841 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
    18901 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
    18961 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
    19021 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
    19081 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
    19141 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
    19201 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
    19261 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
    19321 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
    19381 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
    19441 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
    19501 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
    19561 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
    19621 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
    19681 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
    19741 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
    19801 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
    19861 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
    19921 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
    19981 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
    20041 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
    20101 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
    20161 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
    20221 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
    20281 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
    20341 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
    20401 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
    20461 tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa
    20521 tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa
    20581 taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag
    20641 aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc
    20701 atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat
    20761 aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat
    20821 aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca
    20881 acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat
    20941 cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac
    21001 ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt
    21061 cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa
    21121 gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta
    21181 gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga
    21241 tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc
    21301 accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt
    21361 aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt
    21421 tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc
    21481 gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct
    21541 agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt
    21601 gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga
    21661 ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt
    21721 cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat
    21781 ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa attattttta
    21841 tttaccttga ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat
    21901 tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg
    21961 ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt
    22021 ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt
    22081 cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta
    22141 agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct
    22201 tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt
    22261 ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct
    22321 ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa
    22381 caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa
    22441 ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc
    22501 attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt
    22561 ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt agacggtgta
    22621 ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac
    22681 ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta
    22741 ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg
    22801 attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta
    22861 gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt
    22921 gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat
    22981 ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa
    23041 ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt
    23101 ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt
    23161 cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata
    23221 catttgttcc tatgggattt agagataaag acgggaagct cacaggattt gatattgatt
    23281 tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt
    23341 caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca
    23401 aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag
    23461 tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat
    23521 taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa
    23581 aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg
    23641 atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt
    23701 tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg
    23761 attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc
    23821 tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag
    23881 atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt
    23941 tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct
    24001 cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg
    24061 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
    24121 ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa
    24181 agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct
    24241 catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt
    24301 ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga
    24361 catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata
    24421 ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga
    24481 tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga
    24541 tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc
    24601 cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca
    24661 ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt
    24721 tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg
    24781 tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg
    24841 tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata
    24901 cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag
    24961 acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca
    25021 tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc
    25081 tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat
    25141 tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat
    25201 cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa
    25261 caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa
    25321 gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga
    25381 cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag
    25441 ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata
    25501 cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc
    25561 aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat tactggtacc
    25621 aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta
    25681 cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta
    25741 aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta
    25801 cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg
    25861 gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca
    25921 aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa
    25981 attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta
    26041 ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga
    26101 gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt
    26161 attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg
    26221 gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg
    26281 tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg
    26341 aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga
    26401 aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga
    26461 aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg
    26521 cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc
    26581 agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca
    26641 ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga
    26701 aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac
    26761 agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat
    26821 tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat
    26881 accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga
    26941 acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc
    27001 cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt
    27061 aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc
    27121 aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag
    27181 agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg
    27241 tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc
    27301 tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat
    27361 gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa
    27421 tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc
    27481 gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac
    27541 cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg
    27601 aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat
    27661 gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc
    27721 agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat
    27781 catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg
    27841 gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt
    27901 gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt
    27961 agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat
    28021 gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga
    28081 agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag
    28141 atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt
    28201 tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc
    28261 agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga
    28321 ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct
    28381 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
    28441 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
    28501 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
    28561 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
    28621 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
    28681 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
    28741 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
    28801 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
    28861 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
    28921 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
    28981 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
    29041 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
    29101 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
    29161 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
    29221 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
    29281 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
    29341 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
    29401 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
    29461 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
    29521 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
    29581 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
    29641 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
    29701 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
    29761 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
    29821 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
    29881 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
    29941 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
    30001 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
    30061 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
    30121 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
    30181 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
    30241 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
    30301 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
    30361 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
    30421 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
    30481 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
    30541 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
    30601 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
    30661 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
    30721 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
    30781 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
    30841 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
    30901 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
    30961 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
    31021 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
    31081 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
    31141 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
    31201 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
    31261 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa
    31321 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
    31381 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
    31441 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
    31501 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
    31561 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
    31621 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
    31681 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
    31741 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
    31801 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
    31861 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
    31921 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
    31981 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg
    32041 aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga
    32101 acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg
    32161 tgaaaatggc tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
    32221 aacaccgttt attcagataa gggactgtga caagactttt gtcacagttt ctttttgttt
    32281 attctttttt atctttgtat ggcaataata gacaatataa cgaaaattat ttaatggctt
    32341 ttggaagacc atggaaaacc ttgtacaaag ctattttttc tgaaaaattc cttgaaacct
    32401 cacgaaaaaa tcattatttt atgaaaaaga caaaaaaaag tgttgatttt ttaataatta
    32461 ttcattaaac tcttaaacaa cttcagaaaa tattaattat tctgaaaatt ccaataaagg
    32521 ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact
    32581 tgctggatgt tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc
    32641 agatggagga ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc
    32701 agttgcaaca gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg
    32761 tcttgatgaa gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga
    32821 agacggatta acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc
    32881 tgttacagca gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc
    32941 tgaatatgct tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa
    33001 agataaatca gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac
    33061 gaaacaaaca ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca
    33121 atccgtagtg gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa
    33181 tggaccattc acattagaag attttgatgg accaggaaca gatacagaat ggacttataa
    33241 gaaaaatgat cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt
    33301 agtaaaagaa tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat
    33361 cttgtctggt gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga
    33421 agcgcggaca agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt
    33481 gaacctaaga aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact
    33541 aggtgacgga tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac
    33601 aacgaacgaa gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc
    33661 aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt
    33721 gatggcttct gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca
    33781 agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga
    33841 ccgctcaact tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga
    33901 cccaagcagc tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag
    33961 caatgcagaa tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca
    34021 agcccgttgg gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt
    34081 tccggtgtac caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca
    34141 ccatgcggca ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat
    34201 accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca
    34261 cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa
    34321 tgatcagcca aaagctgtac ataccgtagc tggcagacga gagcgaggaa aattctggtt
    34381 ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac
    34441 ccgaagaaaa gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata
    34501 gatcattaga taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa
    34561 ccagaatcag tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc
    34621 tttatttttg gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctag
    34681 ggggaaacat tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc
    34741 atcacgcttt ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg
    34801 ccatatacga atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc
    34861 ggtttggata aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga
    34921 gattttggaa tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga
    34981 atcggaccat ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg
    35041 attttaggaa cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc
    35101 gtagcgatat taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc
    35161 tttgcaatca aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc
    35221 ttaccaacaa ttgcgttggc catgtcgccg cttgctgact ctgctcggtt tatccgtacg
    35281 gaaatggtgg aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc
    35341 cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta
    35401 ttaggaccgt tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct
    35461 attcctggta tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc
    35521 atggctgtaa cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta
    35581 ttgtacggtc tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa
    35641 atgataccaa agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta
    35701 caaaaaaata ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa
    35761 gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg
    35821 gtcatcattt tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa
    35881 aacgtggctt atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat
    35941 ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca
    36001 aacgtaaatt tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt
    36061 atgggaatga gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt
    36121 ggtgtagctt acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa
    36181 cgttttttgg aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc
    36241 gtttttgaac caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca
    36301 atggcacgta tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct
    36361 ggattgacat taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc
    36421 tctagtgtca ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc
    36481 tttttaagct ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg
    36541 agcgaagggt acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact
    36601 ttatcggtta tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat
    36661 cctaaaatga aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct
    36721 cttttgatac gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata
    36781 aaggagaaac attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa
    36841 gcatcatgcg cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag
    36901 gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag
    36961 cgatgatttt ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag
    37021 tcgctgagtc ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat
    37081 tagaattgct taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc
    37141 atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc
    37201 cggaaatcct gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga
    37261 ttttggagct gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc
    37321 atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa
    37381 tcgttgaagt tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg
    37441 gactgcttgg ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg
    37501 gttctccacc ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat
    37561 tcgcgatgaa aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca
    37621 aggcagcaac ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc
    37681 aacgtcgctg ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg
    37741 ctgatgccaa ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa
    37801 aagaatgaag tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc
    37861 ggtttagttg gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg
    37921 aatgaaccta ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat
    37981 aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct
    38041 tcgttgaatc ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga
    38101 ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga
    38161 ttgaatccaa gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt
    38221 attggtattg cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt
    38281 tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa
    38341 gaacatcaat tgacctatct atttattgca catgatttgt cgatggttaa acatattagt
    38401 gaccgaatcg gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc
    38461 tataatcacg gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat
    38521 ccagaccatg agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa
    38581 gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc
    38641 ccatattacg caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag
    38701 ggacatagat gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc
    38761 aattgattcg tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc
    38821 agaaaagttg gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa
    38881 aagaataccc attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt
    38941 tggatcgggc ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga
    39001 aaaaaagtat gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa
    39061 atggctactt aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag
    39121 ttgaaggatc gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg
    39181 cactactttg tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg
    39241 cctatcatcc gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat
    39301 ttcaccagac agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct
    39361 ccgctcacca tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa
    39421 aagtaatgga tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat
    39481 ctgggaaatt ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg
    39541 cacatgctga tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt
    39601 cccaatggta taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt
    39661 ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa
    39721 aaatgaatga aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac
    39781 ttttgtttct aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa
    39841 acaatagcag aaaaa
//
LOCUS       DAFBQW010000020        34589 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFBQW010000020 DAFBQW010000000
VERSION     DAFBQW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.021"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            212..604
                     /locus_tag="LK889_001319"
     CDS             212..604
                     /locus_tag="LK889_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5095562.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LK889_001320"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LK889_001320"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBK5095563.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LK889_001321"
     CDS             3165..4775
                     /locus_tag="LK889_001321"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBK5095564.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LK889_001322"
     CDS             5067..5936
                     /locus_tag="LK889_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBK5095565.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LK889_001323"
     CDS             6101..7372
                     /locus_tag="LK889_001323"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK5095566.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LK889_001324"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LK889_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK5095567.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LK889_001325"
     CDS             8763..9026
                     /locus_tag="LK889_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBK5095568.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LK889_001326"
     CDS             9471..9920
                     /locus_tag="LK889_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5095569.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LK889_001327"
     CDS             9977..11395
                     /locus_tag="LK889_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5095570.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LK889_001328"
     CDS             11395..12255
                     /locus_tag="LK889_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBK5095571.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="LK889_001329"
     CDS             12429..13202
                     /locus_tag="LK889_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5095572.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="LK889_001330"
     CDS             13349..13714
                     /locus_tag="LK889_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBK5095573.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="LK889_001331"
     CDS             complement(13862..15070)
                     /locus_tag="LK889_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5095574.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="LK889_001332"
     CDS             complement(15089..15367)
                     /locus_tag="LK889_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095575.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="LK889_001333"
     CDS             15521..16216
                     /locus_tag="LK889_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5095576.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="LK889_001334"
     CDS             complement(16658..17455)
                     /locus_tag="LK889_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBK5095577.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="LK889_001335"
     CDS             complement(17452..18636)
                     /locus_tag="LK889_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK5095578.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="LK889_001336"
     CDS             complement(18661..19908)
                     /locus_tag="LK889_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK5095579.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="LK889_001337"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="LK889_001337"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK5095580.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="LK889_001338"
     CDS             21888..22202
                     /locus_tag="LK889_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK5095581.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="LK889_001339"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="LK889_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK5095582.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="LK889_001340"
     CDS             22887..23531
                     /locus_tag="LK889_001340"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK5095583.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="LK889_001341"
     CDS             23546..23875
                     /locus_tag="LK889_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBK5095584.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="LK889_001342"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="LK889_001342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK5095585.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="LK889_001343"
     CDS             24863..25687
                     /locus_tag="LK889_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBK5095586.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="LK889_001344"
     CDS             25680..26027
                     /locus_tag="LK889_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBK5095587.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="LK889_001345"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="LK889_001345"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK5095588.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="LK889_001346"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="LK889_001346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK5095589.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LK889_001347"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LK889_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBK5095590.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LK889_001348"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LK889_001348"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK5095591.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="LK889_001349"
     CDS             31657..32451
                     /locus_tag="LK889_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095592.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="LK889_001350"
     CDS             32441..34483
                     /locus_tag="LK889_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5095593.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11118 a         6130 c         7260 g        10081 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcgcgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttata
//
LOCUS       DAFBQW010000021        34480 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFBQW010000021 DAFBQW010000000
VERSION     DAFBQW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.022"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            278..787
                     /locus_tag="LK889_001351"
     CDS             278..787
                     /locus_tag="LK889_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5095594.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LK889_001352"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LK889_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK5095595.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LK889_001353"
     CDS             2322..3302
                     /locus_tag="LK889_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5095596.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LK889_001354"
     CDS             3424..4842
                     /locus_tag="LK889_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBK5095597.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LK889_001355"
     CDS             4913..6382
                     /locus_tag="LK889_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5095598.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LK889_001356"
     CDS             6392..6859
                     /locus_tag="LK889_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5095599.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LK889_001357"
     CDS             6895..7767
                     /locus_tag="LK889_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5095600.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="LK889_001358"
                     /pseudo
     CDS             7848..10382
                     /locus_tag="LK889_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            10538..11311
                     /locus_tag="LK889_001359"
     CDS             10538..11311
                     /locus_tag="LK889_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5095601.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="LK889_001360"
     CDS             11304..12095
                     /locus_tag="LK889_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5095602.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="LK889_001361"
     CDS             12105..12596
                     /locus_tag="LK889_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5095603.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="LK889_001362"
     CDS             12614..13024
                     /locus_tag="LK889_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5095604.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="LK889_001363"
     CDS             13028..14428
                     /locus_tag="LK889_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBK5095605.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="LK889_001364"
     CDS             14451..14825
                     /locus_tag="LK889_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5095606.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            15314..15508
                     /locus_tag="LK889_001365"
     CDS             15314..15508
                     /locus_tag="LK889_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095607.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            15498..15851
                     /gene="tnpB"
                     /locus_tag="LK889_001366"
     CDS             15498..15851
                     /gene="tnpB"
                     /locus_tag="LK889_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK5095608.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            15953..17500
                     /locus_tag="LK889_001367"
     CDS             15953..17500
                     /locus_tag="LK889_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK5095609.1"
                     /translation="METTDTLLQLLQEAHKTNQTQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKTDKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            17652..18080
                     /locus_tag="LK889_001368"
     CDS             17652..18080
                     /locus_tag="LK889_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5095610.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFLI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            18067..18309
                     /locus_tag="LK889_001369"
     CDS             18067..18309
                     /locus_tag="LK889_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095611.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(18609..18824)
                     /locus_tag="LK889_001370"
     CDS             complement(18609..18824)
                     /locus_tag="LK889_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095612.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            18931..19245
                     /locus_tag="LK889_001371"
     CDS             18931..19245
                     /locus_tag="LK889_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095613.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            19349..20527
                     /locus_tag="LK889_001372"
     CDS             19349..20527
                     /locus_tag="LK889_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5095614.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            20930..21223
                     /locus_tag="LK889_001373"
     CDS             20930..21223
                     /locus_tag="LK889_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBK5095615.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            22066..24393
                     /locus_tag="LK889_001374"
     CDS             22066..24393
                     /locus_tag="LK889_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5095616.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            24519..24848
                     /locus_tag="LK889_001375"
     CDS             24519..24848
                     /locus_tag="LK889_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK5095617.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            24888..25892
                     /locus_tag="LK889_001376"
     CDS             24888..25892
                     /locus_tag="LK889_001376"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5095618.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            25889..26335
                     /locus_tag="LK889_001377"
     CDS             25889..26335
                     /locus_tag="LK889_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK5095619.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            26485..27300
                     /locus_tag="LK889_001378"
     CDS             26485..27300
                     /locus_tag="LK889_001378"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5095620.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNMI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            27506..28678
                     /locus_tag="LK889_001379"
     CDS             27506..28678
                     /locus_tag="LK889_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5095621.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(28840..29334)
                     /locus_tag="LK889_001380"
     CDS             complement(28840..29334)
                     /locus_tag="LK889_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095622.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(29331..29525)
                     /locus_tag="LK889_001381"
     CDS             complement(29331..29525)
                     /locus_tag="LK889_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095623.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            29920..30342
                     /locus_tag="LK889_001382"
     CDS             29920..30342
                     /locus_tag="LK889_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095624.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(30508..31701)
                     /locus_tag="LK889_001383"
     CDS             complement(30508..31701)
                     /locus_tag="LK889_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5095625.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LDFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            31925..32302
                     /locus_tag="LK889_001384"
     CDS             31925..32302
                     /locus_tag="LK889_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5095626.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            32290..32628
                     /locus_tag="LK889_001385"
     CDS             32290..32628
                     /locus_tag="LK889_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5095627.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            32762..33205
                     /locus_tag="LK889_001386"
     CDS             32762..33205
                     /locus_tag="LK889_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBK5095628.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            33467..34282
                     /locus_tag="LK889_001387"
     CDS             33467..34282
                     /locus_tag="LK889_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5095629.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            34292..>34480
                     /locus_tag="LK889_001388"
     CDS             34292..>34480
                     /locus_tag="LK889_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095630.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
BASE COUNT        11834 a         5494 c         6804 g        10348 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaat
     8881 gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat
     8941 tgaagaaagc atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga
     9001 gtataattat ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat
     9061 atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg cggatatcag
     9121 tctatttata atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa
     9181 gttcagaaaa tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag
     9241 tagtttagaa gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt
     9301 aaagaagttc tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat
     9361 aacagcttct gcggatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag agtaagcgcc
    15241 ccatagaaca gtaccttact aacaaatgca cctcaagata tacttgagct atcaagaagt
    15301 ctggaggttt tctatgtata aattaaatga gattgttcct gtccaattag gaaacgaaaa
    15361 gagtgcacca gaaaaaggta aacccgcggc tgccaagtta gcgatgcgca tcaaaagaga
    15421 gaatggttct gaagttttga tctataacgg tattaacaat tatattcttc aagctatttt
    15481 gaaagaactg ttgtccaatg ctttatgatt tacttcaaac caaacatatt tatattgtgt
    15541 gcggaaaaac agatttgcga aaaggaattg atggtcttgc cagtcttatt caacaagagt
    15601 atcaactcga gctttacgaa gatgccgttt ttttattttg cgggaaccgt caagaccgtt
    15661 tcaaacttct ctattgggac ggtgatggtt ttcttctctg ttataagcgg atagaaaacg
    15721 gcaagttaaa atggcctcgg acgaaagacg aagttcgaac cttaacgaac caacaggttc
    15781 gatggttgtt agaaggatta agtatcgatc aacctcgagc tattttgcca ggaaaaaaag
    15841 gagtttttta agctgggggg ttaacacaaa aaatgtgttg aacccctatt tcttgtgcgt
    15901 ttcttgtggt attctgtaac aaaatcagtt cgattcaagg tggtgaaaaa ttttggaaac
    15961 tacagatacg ttgcttcagt tgcttcaaga agcccataaa accaatcaaa cacaacaaca
    16021 aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca attatttaac
    16081 caacaaatta tttggacgct ctaaagagac tctctttgaa gaaacgaatg ggcaattgaa
    16141 tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca ccattattcc
    16201 agtaaaagga catcaacgag ttgtaggaac gaaaacagac aaaatcaaac acttacctat
    16261 aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt gcggttcaca
    16321 gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc aggctatgtt
    16381 ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa aagaaggact
    16441 ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct taggttccaa
    16501 tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg cctatcggca
    16561 agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata tcaccaattg
    16621 gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa gagtctatct
    16681 taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg aaagtgccaa
    16741 aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc ccatcgttta
    16801 ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca aggagtttac
    16861 agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg tacgacttgt
    16921 ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag gaaagaaaaa
    16981 cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg tcctagaaaa
    17041 gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag aactgcgtcc
    17101 attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa aatccaagtt
    17161 ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta tttttgaaga
    17221 tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc tggtgatagg
    17281 acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag gaatcattct
    17341 gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc tagagtttct
    17401 atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc aactgttgcc
    17461 ttggaacaag gatgtacaac aacatttttc tcgtaactaa aaacgccaaa cattcccgat
    17521 tctattctat aggtcgggtt atttgacgta caggtactgt tctatggggc gcttacctcc
    17581 tagattgtag taataatgga aagggataga agtaaaacga gttgaatgaa ttttttgagg
    17641 gggagaatat tatgggcgac tcagaggaaa cccacattca acatacgttt gacagttttt
    17701 gtaagaaagt agtctgcaat gaggcgttaa atattcaaaa gaagtacgct agatttcgcc
    17761 aacggcaaat atcaatggaa atattggaac aaaaagggtt aggtacagag ttctattatt
    17821 tggaaccaaa tttgaatagt agtggtactt ttattgtcct tggtatgaaa atctttattt
    17881 ccaatgagga gttggcaaat gcgattgcag cattacctag tcaaagacaa gaaatcattt
    17941 ttatgtcctt cctcatcggt cttaatgatc gtgaaattgg cgatattatt gggaaaagtc
    18001 tcggcagtgt ttggtaccaa cgacaaatgg ctttggaaga gctaggaaag tatttgggtg
    18061 tcaaatatga agcaaaataa acgatatata ccattcatac cactaacgac aatcaaagcc
    18121 gcagcttctg gtgattcaat tgccatgtcc atcgttctgt caaaatatca aaattatatt
    18181 tcacggttag ccatgaaacc agtttatgaa gaagaaaaag gaatggttat gtatgtagat
    18241 gaatatatgc gtagacaact ggaaacaaaa ttgattgaga agatacttga atttgataca
    18301 actcgttaaa gtattttttc cctttccttt tcattctatg gaagtttttt atcgggtcaa
    18361 aataaaggat ttccataggc tgaaaatagc ctagcgctct ttgacaaatg aatagcaaat
    18421 ctatcagtac atgtagattt tgattctcta gttacgccat gacaaggtta gcttttaagc
    18481 gacaaatttt aatatgagaa tcgcgactac gataatgata cttccgtaac caaatcggcc
    18541 cgccattact ggggggaggt gagattccta tgtgctttgg caaaacagct gatagatcag
    18601 aaaaactact atttccatat ttcgattttg gttggcatat ttttcatgcc aaattctgtg
    18661 attttaatag catcggcaag atttaatttt tcttcagctc tctctccact tttaagtttt
    18721 tttactgtac tcaagttgat tcctgttccg aatgctattg cattagccgt agatttttca
    18781 agaatttctt taattatttt tatgtctgca attccttgtt tcatattaaa aattcctctt
    18841 ttctagtccg ttttgatgga gtactggagt gtttttttac aattttaagg ggtaccgtgg
    18901 tggtcgtttt gattttgaga gagaagggtg atggagttgg aagttaaaac aaatgttaat
    18961 tctctggaaa tagagttcta ttcaatttta caaaaaaaac tggaagatta cacggaaaaa
    19021 ttgcttgcag tcagtcattt acctctggta ttaacaaata ctgatttaaa gcgactattt
    19081 cagataagtg atagtacact gaaccgttta gtaaaacttg gtgagtttcc tccctgttgg
    19141 tacgggattc gaggacacta ttttcgtgat gatgttttag aatggatgag aaagagtgat
    19201 tcgaatgact atcgagaaaa attgcgacta cttcgatctt tataagttac attttctttc
    19261 taagatgcgg tacaatgatg acatagatag aaagatttcc aaagggtttc tgcttgttgg
    19321 gttccttcaa aataggagga atcaataaat ggaaaaaaca gatcgattca agaagcaaat
    19381 cagttctact ggtaggatca catggtactt tcaagcatat cgaacaacaa gacgtggctt
    19441 taattcaaaa cgtgaggcgc aactcgctta tctggaaatg gagaaacaac aaaggcttaa
    19501 gaaaaaaatt gttgcaagca atgacaagtt tagtgttgta ggtgagcaat ggttccaata
    19561 ttaccgttcg ttaaatgaac aaaaggaaat aacctatgat aagcgagagg aacaattaaa
    19621 aattattgtt cgatggattg gtgataaaaa gttgtctgat ttgacaccag agtgccttca
    19681 agaattgctt tttaccttaa aggaatgtgg cgttaatgga acaaatgttg gctatgccaa
    19741 aaatagttta caatcactta ttcaagttct aaatatgatt tttaaatatt gtcaaagaaa
    19801 gaatttgata agtgaaaatc caatgaagtc tgtgagaatg ccaaaatatc agttaacggt
    19861 aactgattta aaagaagcca taaataatat tgaggacaag tttcttactg tcgatgaatt
    19921 gagaactttt ttgaattatg gcattgttca tgaggaatta ccgatgtctg tacttttcca
    19981 tgttctattt tacactggct gccgagtcag cgaggcgctt gctctacagc ctggagatat
    20041 agatttcaaa agaaatgaaa ttctgttcta taagcaaacg gcagttaaag gtaagagtaa
    20101 ggattttcga attgaaacta ctaagacagt tagttctgct agacgagttc tagtaactcc
    20161 tctagttatg gaaaagctac aacagttgat taaggctttg ggtgagatga ggcaacatta
    20221 caggtttatt atagaagaaa catatttgtt tgtgtatctt gattctagta aacgtggtgt
    20281 tccttatcgt cgagagtatg taaatgatca cgtcaaacgg tgcgtgaatc gctcaggaat
    20341 taataagcca tttcatactc atttagctcg tcatacaatg gcaagcttag tagcggaaca
    20401 ctgtagctgg gaagtcttga aagatcgctt aggccatact gaccaaacaa cttcgaaaat
    20461 ttatcgccac ttaacgtcaa atgaaaaggt aaaacctctg attgctttta gttctttaga
    20521 gagttaaaaa aacggtgaaa gcactggtat tacaggattc ttgaaaaacg attttttaac
    20581 aaattagtca atatgtagtc agaggtaata aaaatccttt tatatcaata ctttaacgac
    20641 ttttcacaag tatcaaagaa atccgtcgtt aatcattgtt atcagactga gaaagaaccc
    20701 ttggtattca agggttcttt tttcttagca tgtaatttga acaccctgat ccagtttttt
    20761 ctttaattat tggtagggga gaataatgga ttagaataaa tctacctata agtagtagta
    20821 aaaaaaagag cactatgata atatctttct taagagtaac cgctttaata aaaaatccag
    20881 aatattctaa cataaatgga gtattcttag aatcaaaagg agaaagaaga tggtttatac
    20941 ttatacggag caagaaaaga tgaaagtttt agacacattc atgaatgatg gaaaagtaaa
    21001 acaagtacca agttcaacaa aaaagaaata catcctattg aaagaagtct tgaaacgctt
    21061 tgaccatgga gtgacttata cagaaacaga ggttaattcg atactgctga acgtattttc
    21121 tagtggcgat tatgtcgaac aaagacgtta tctaatcaca tttggctttt tcaaaagaag
    21181 ttcagacggt cgggcatatc agatgatggg aattgaaaat tagaagtttt aatttatact
    21241 cattgaaaaa agttcaagta tgcgatattg gttctacacc gcatacttga actttttttg
    21301 tcatctgtat ctataataca aagccaagaa cgttattcaa aatatggata gctatgataa
    21361 aagctaatga gcgattcgca gttaaataag caatcgtcag taaaatcgtt ccaggagcgt
    21421 acatgaaaaa atcaattgga tgcgttacat gaataaagat aattctcttt tatttgtacg
    21481 aataaatacc aaaacgtacc tcctaaaatt agattttgga cagatgccct ttaaaaagat
    21541 aacaagattt cgttcttgaa acaatatata tgcttttatt ccaatgaggt tgtgaaaaca
    21601 gtattttgtc ctgagcgtta gaaaattgaa aatggggaat agcaactcgt actttaccat
    21661 tttttaagtg aatacaagat cagctaattg aacaccgttt attcgtgatg agggacacaa
    21721 cactgcagtc gacacatttc tcttttatgt tcaaagataa caaatattta aaacaaagaa
    21781 aaatagtaga taatcccgtc ctaactcttt tattcttccc tcatagcttc caaatttaag
    21841 caatacttca attaatcaag ttttatcttt ttttttcaat aatcgtaaaa aaagagtatt
    21901 ttttatttaa aaataaaatc tttgtattaa aggacaagac taaaataacc ttagaataaa
    21961 aagagaaaaa aagccccaaa ctttctatac atcaataaaa taataaaaat taaatttaga
    22021 ataacgtgta aaggattatt tgatgatttt ttattgagag gagggatgaa aatgattaag
    22081 aaaactcaaa ataaaatcca acgtatatgt caaaaacatt ttgtttctag actcaaagct
    22141 gtatctaaaa gaaaatactt acatcccatt atgatttcga tcatcttgat ttttactttc
    22201 ttagggagct tatcaagtat ttatgtaaac gtttatacag aaacgtcaaa ttttgctaac
    22261 caaacgacag aggagacaaa tagtccaccc tattctgaaa tacagtccat agaaagcacc
    22321 aatcagtcta gcaacaacga gacaacctca accacatcct cgactcaact gattgatccg
    22381 acagaaaaag aaatcacagc agcaaaagca gcaggggaga aagcttattc agaaacaggt
    22441 agaagccaga catttagtac tgttgcggcc actgcgaatt tcaacggaca taccatacca
    22501 gatggatata cagtgatata tgccaaagat ggagtcattg ttgcggttaa cggatcgatg
    22561 agtgcggata aaactgcgtt acaaggcgta accaaagcaa tcgcttttat tgataaccgt
    22621 ccagatatcc ataatctgtt tattggctcc acagtaaatg gtactactat cacattccca
    22681 aaaagtatct atatgggggt ggctaccgct ggaaatacga tcgattcagt tacacccaat
    22741 gcggataaaa gttattcttt tacaccctca acatggggag ggcttcctgc cggaggaggt
    22801 atgagtactt tttcaatcgg tatacagtta gcaaatgata ttccattcac ggggtactcg
    22861 atcaaaaatg ggcagatcgt tgatggtaag ggaaatgttt caaattttcc aacctatcaa
    22921 gcagcaacgg ttcaggtcaa tgcgaatcgg acaggaactt tttcttatac agtcaatggt
    22981 ggagcagcaa aaatcggaac gtacgggact acattatctg gaatccatgt gggtgacaaa
    23041 gtggttatca ctccaacagc agtaacgggc tattcttttg cacaaacaaa cggttctttc
    23101 acagccctag aaagtaacgt cgatacggtg acctatacag gcaaacaaac accaatcact
    23161 attaagtatc aagactattt tggacagatg attgctcctt ctaaaataat gaatcttact
    23221 tacggaagtg ctgcacaaga cctgacgact aatacgccta ctatcaacgg atataccttt
    23281 acagctgttt cagcgataga aacaaagaac caatcagcaa tgagtgtttt agctagttta
    23341 gatacaaatg gaaatgtaat cgtaaaggac gcaaatggaa aacagatttc tgaagtcatc
    23401 ctatattata agacaaatgc aacagtatca atcaatgcaa atggcagtaa gtactatgat
    23461 ggtctttctg ttgtaccgat cgttaaatat gcctttaatg ataaaacaga atcaacatcc
    23521 cttttaaaca atgatctcag tattgatcaa atcacatgga atccaactga tttcaaagcg
    23581 atgaatgaaa agggagttga agtaactgcg cctactgaaa ttggaaccta tacgtcttgg
    23641 caattgacaa aaagcggact agctaaacta gctgccagaa caaactattt atttactatc
    23701 gtgcagacat ctgatgtcta tacgattaag caaatcagcg gtgcggtaga gctcagtggc
    23761 agtaagacat atgatggaaa agcaggtgtc ccgtcaatcc atgtcaagct agcagagggt
    23821 gtcactagtt cgcttactcc agtacaagtg gcacttagca gtatggatta cacagcagat
    23881 gcacaatcag caaaaaatat ggttaatgct ggcagctata caattaaact aacgaatagc
    23941 ggaatagaca aagtcaaagc agcaaatccc aagttgtcat ttactgatat agccaacaca
    24001 tcaggaactt ataccatcta taaagctaac gcagtgatta ctgtcgatga cgctgccttt
    24061 aattatgatg cccaaagtca ctcgatttcg gttggaaatc tccacgttac cggtgttgtc
    24121 ttaggcgaat cattggatta taccctaaca gttaacagcc ggacagatat tggatcacaa
    24181 atagtaacga ttagtttagg cgaaggaagt gtaaacaata attatacgat tatcgcaaca
    24241 gataccgcta agttgacgat caatccgact ctcttgcttc cttcgacagg tgggataggt
    24301 ctactgccct ttgtcttttt aggactgatc tttatttttt ctggcttctt ttattttatc
    24361 catcgtaaga aaaaagcagg tgaacagcga tgaaaaaaac aaaaaatgta cgtgtggata
    24421 tatttgctgt tctctttttt atcctactgg tcttttttat aggttctccg actgtgtcag
    24481 ctagagaatc acctattaca tttactttgc ataatcgaat gttcgaaagc gataagatgc
    24541 caagtatcag tcaaaacgac ggaataaata tcccggataa ctttcaagga gtaaaagggt
    24601 tgaacggcgt tactttcgct atatatgatg tctcggatga attttacaag ctgcggtcag
    24661 aaggaagtag tgtggaagac gcacaaagaa aactagcaca aaaaagtgat agtgaaaaaa
    24721 tactggcaga aaatgtgaca aaaacagtag acgaggaaga gggcattgct tcttttagtg
    24781 catcggacaa agatgagcaa ggtcgtgacg ctgtttaccg atttacggaa ataaagactt
    24841 cagattaagt aaaagaaaaa tcagcaccat ttgtcgttgt gcttcctgtg acagattcat
    24901 ctaatcataa attgacgacc atccacttgt atccaaagag cgaacaaaaa atacctgctg
    24961 cactcacatt aacaaaaaca gtagagaata agcagacaga ttttgcagat ggggataaag
    25021 tcccttatct catcaccgta acaattcctg agaacatcaa cgagatagga acctatacga
    25081 tcaaagatac agctgatcct cagttatggc tagaaccaga aacgatcgat tttttgattg
    25141 gtggagacaa gattcataca tttcacacac aaaaaataaa acatggtttt gtcttatcag
    25201 attctggaaa agatcttagt tcgtttgctg gaaaaaaatt aacgatccgt taccaaatga
    25261 cattgaaaga aaacagtgaa aaaacgacat ttgataacga tgtacgtttg acgactgaca
    25321 atcaaacagt agaaacacat cttgccatcc aaacaggagg caaacaattt gtcaaagtgg
    25381 atcggcaaac gaaacaaaaa ctagcagatg ctcaatttct agtcaaaaaa aatgaccaat
    25441 acttagcgca agaaaacggg ataaatcatt gggtagccaa agatgattca agagcaacga
    25501 tttttacttc cagtaaagat ggaacatttt ctgtccatgg attgtctttt ggaagttacg
    25561 aactagtgga gattaaaccg cctaaaggat atatattgag tcaatcaacc attctctttg
    25621 aagttgaaaa aggcagttat gatccgcttc atcctatacc attggaaatc atcaacgaac
    25681 caaaagcaaa tccgacgaat aatggaaaaa cgccagttca gatgcagaca aatggtccat
    25741 caaatgaaaa aaatggagga agctttccaa aaacaagcga agagatgctt ggaggtttct
    25801 caattttagg gttattgcta gtcctttcaa cagggacagc ttggttttat aaaaaacaac
    25861 aaaaaggaaa caataggagg gaaataaaat gaaaaacatc aaaacaaaaa tatggacgtt
    25921 tttaggaact gctatcatgc tgcttccttt cgttctcggc ctaggaactg cagaggtttc
    25981 tgcagccgta tcacctacac cagaaaatgt tacggttaat ttgcataaat tgaaattcac
    26041 ttcagcacca gaaaatcaaa taaacaacgg aacggaattg acatttccga atagcgaacc
    26101 gttgaacggc gttgaattca acgtatatga catcactgct acgtactatc caagtaaaga
    26161 cacagcagtt ccagcagacg ctacaccctt cgcaagtgta acgacttctg gtgaaggatt
    26221 agctaacctg actcttccag gtaaatctga tgggaaagat gctgtatacg tctttgtaga
    26281 aacaccaaag cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac cctagaccct
    26341 gacaaacctt tagacacgat tcacctatat gctaaaaatg tcatcaaaga aagcaatatc
    26401 aaaatccaga aagctgggaa cttttcaggg gtagataagt tagcaggggc gcaattccag
    26461 atcaaaaatt cagagaatca attcgtgacc ggttttgatg aaaacactgg tgtcgctctc
    26521 tatgatcctg ctgagtcagc gttgacattc acaactgata ctgaaggatc catcagtgtc
    26581 aatggattaa tagacggcaa ttatacttta gttgaaacaa aagcaccaga tggttatcaa
    26641 ttaaccaatc cacaaaaaga aactactttt atagtaaaaa acggaaaatt gcaacaaaca
    26701 accggtatca tccatggcgc agatgaagcc aatcttggga atccaaacgg tgtagtcaat
    26761 cgggaaaata caactacgga taacatgatt actgtagaaa atgtccaaaa ctacggtgat
    26821 ttccacttca tcaaacaaga tgtgaacacg caagaaaaat tagccgatgc agaattttct
    26881 attctaagag caaaagatcc aactgttaaa ttaatgaaaa aaatagcaaa ccttgcctca
    26941 ggagaatacc catatgcttg gtcagatgaa gtcacaggtt ctgactggga agaagtgaaa
    27001 ttgatttctg attcagaagg gaaattttca atcgaagggc tgaaacaagg ggcatactgc
    27061 cttcaagaaa caaaagcacc agaaggctac gcattaccta agaatgaagc tgcgtacact
    27121 gaatttacag tagatgatga tccaacaact tctgatgaaa caacgatcga taaccaacct
    27181 aaaggaatct tgccttcaac tggtggtatg gggatcatcg tattcatctt ggtaggtact
    27241 gctttagttg gaggagcagt gatctatttc aaaaaacgcc atcaagaaac agaagcttaa
    27301 actattggaa acaaaaatca tacgataaat aaacgatagt gattcaaatg gggagaaagg
    27361 ttgtgacaag tggtcggtct cttggtattc gctcaacaaa cggcaaaata ggaggaacta
    27421 ttttgccgtt tgcttcgcta atactcaggt caaggcgctg atttcacaac ctcttcatat
    27481 acatattaga aaataggtgg acatgatgaa taaaagcaga ataagaatac tggacatctt
    27541 catggcaatc attttagtcg ttgggatcgg gatcttttct tatccttttg tagaagattc
    27601 actgaatgat tttcttgccc agcaaatgat catccattat caaaagcaag catcgaataa
    27661 gaattcagag gaaataaaaa aacagcagga aaaaatgaca aagaaaaacc aacagttggc
    27721 agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg caaaagcatt
    27781 aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca tcgaaatccc
    27841 aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt tgcaaaaagg
    27901 aacgtcatta ttggaagaaa gttcttatcc gactggcggg aaaaacaccc atgccgttct
    27961 ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta agttgaaaaa
    28021 aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag tagaaaaaat
    28081 ccaagtggtc ttacctgatg aagtagattc tttgggtatc caaaaaggca gagatctcgt
    28141 gactttgctt acctgtactc cttatatggt caatacacat cgactgcttg tgacggggca
    28201 tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg atcaatggaa
    28261 aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg gcttagtttg
    28321 gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc ctttatcttt
    28381 ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg taaaaacgct
    28441 caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt ctgatgaata
    28501 cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac atgaggagct
    28561 cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga aattgaaaaa
    28621 aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga aagaatgaat
    28681 gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa gctgacgaaa
    28741 tggacgtgta aactgttatt tttggtttac tatctggaac gccatcttct atttttttat
    28801 gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat aacatttgga
    28861 gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat acgtaccatt
    28921 tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag tttgttcatt
    28981 attttgctca ggagctgcgg cttgtgcctg ctgagaggcc tgttcagctg ctgattgctg
    29041 tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgc ctcgcctgtt ctgcttgttg
    29101 ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt cttcttgctt
    29161 tttccgttct tcttctgctt cttttgcttt gattgtggaa tcaattgcag caagacggct
    29221 tgtcaactct tgtttgtttt caggtagcgc ttggatagct gctgaagcag ttgcatagtt
    29281 ttcttttgta ggatttgctt cagcttgtga caaagcagat tccgcttctg tcactttttt
    29341 tctagtgcag ctttttccgc tttttgtttc tcttcttttt tcttttcaat ttgttcttgt
    29401 tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga agcctgatct
    29461 gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa acttcctgcg
    29521 accaaaaaca atgcaactaa ttttttcgct aaatttcctc cttaagtaaa aaacaaccgt
    29581 tttcaataaa tgtccttttc aattgtttac ttacttgtta taataattgt gttatgaata
    29641 gtgattgagg aaacttaatc taatcccgtg ttgatacacg ggattttttg ctatagttct
    29701 gttattaagt atgcaataat aaaatatgta cgttatttta ttattagatt attgacattt
    29761 atataataaa cgattaaatg attcatatca atagaatact taggaaaaaa agagaaataa
    29821 taacaggttt atgcactatt gacggtgtga caatatgata tttgtatccg ttttttttct
    29881 tgtatgtagt atagtaaagt agatacagga ggagaaaata tgcagacttt tttaacaaaa
    29941 gtttctcaaa agaaaattaa aatgattcat ttactgcttg aaacagaacg ttggtgttca
    30001 atagaagaac tacaaaacga attgaaggta tcaagtaagt ctatcttgaa ttatctaaca
    30061 gatttagaag aattgttcca aaaatatcct gataaggtca ttttgaagaa tgagaataat
    30121 cggcgctttt ctatggaaaa gcaggagaac ttcccaatct acattattta tcttcatttc
    30181 tacagaaaat catataatta tcatttgatt gaattcatgt atcaacatcc tgagaaaaat
    30241 ttggaagatt atgcagacgc tcaatatacg agtgtatcca cagtttttcg ttatgctaag
    30301 ttattagtaa agtactttga aatgacggac gtcaaagaat agtcgccgcc agatagaagg
    30361 aaattagcag ttgaaaaagc tgccctgcat caagtaatac gaccagccaa acggaaacag
    30421 tgtctcatgt ttttgatttg gctggtctta ttaccgcaga tgcaagcttt tgccacacct
    30481 ctttcttgtc atttacagac tcaactatca atgctgcaag ctttccctta atgctgtaac
    30541 accagtaaga gttgtcaaga ccagcagact gactaggtaa ataccgctca aaaacaagat
    30601 tacggcagtt aaactataat gatccatcaa gaaaccgatg acgatagaag aaaaaccacc
    30661 aacagctcgt ccaaggttca taatgaaatt attcgccgtc gcgcggattt ctgttggata
    30721 caaactacta atcactgcgc catatccacc atacatgcca ttgataaagt agccgactat
    30781 cacagcggct gctaccaaac tccactggct atgggctaac acaagtagat agacagataa
    30841 ggatgaacaa attaaaaaca aagaaaagtc tagtttaggc cccaatttat ccatgattat
    30901 gccaaaagtc atcatcccca aagacatgcc taaaatagta ctgaccatcc acaatgaaga
    30961 cccagaaacc gaaagcccta atcgattttg cataattgaa ggcagccaat tcattaaccc
    31021 aaaatagccg gcaatttgga cagttaccat gatactaagg ccacatgttt gccaagcaag
    31081 atgttttgat tcaaacagtc tgctaagttt ggttttcttt tcggcagatt gtacagattt
    31141 gaattccttt ggttcagtca agccgcgacg aatgaacaag accatcaaca ccggcagtac
    31201 acctacgaca tacaacatct tccaaccaaa cgttggaatg atcacagcag ctaaaatcgc
    31261 agccaaagcc gcaccgacct gcgcaccaat accagcaacc gaagaagctc gtccaatttg
    31321 tttttttgaa aaactttcgg aaatcaagga catacacgcc ccgtattctc ctcccgctcc
    31381 tacacctgcg ataaagcgaa agagataaac caagtagatg tttgaagcaa aatacatcaa
    31441 taaagaagca atcgaaaaca gaaggaccgt ttgagaaaat actttcacac gtccatattt
    31501 atcagctatg atgccaaaaa aaatacctcc aatgagcatc cccaaattag tgatcgttga
    31561 aatgaaaccc gcttgcgtac cactaatatg aaaagaagta atcattgaag ataatgaaaa
    31621 agctaaaaac ataatatcca tattctctaa agcaattccc gccgatgtcg ataataatac
    31681 ctttcgctga tacgttgtca tttttatacc ccttttccta aaatttccct aaattgttaa
    31741 aagtatatcc aaaaaagaat aaaaaaacaa gactttttta tttgaaataa tacacaaaca
    31801 accgatatag ataatccagc ttagttttaa tagaaatgag tattgacttg gagtacactt
    31861 aaagttgtag aataaataaa atacataagt aaagtggcaa gaaattaaga gaaagagtga
    31921 tcagatgaat atcaaacaag tcagtgaaga aaagggcatt tcggccgata cattgcgcta
    31981 ttatgaacga atcgggttga ttccaccagt taatcggaca aaaggcggaa tccgtgatta
    32041 tacagaagaa gacctaaaat gggtcgactt tacgatctgt atgcgaaatg ccggattatc
    32101 gatcgagtct ttgattgaat atattcgcct atctagcgaa ggagaagcaa ctgtatttga
    32161 gcggcgtaag cttttgattg aagaaagtga acttttgacg aaagaaattg ctgaaatgca
    32221 agagtgtttg gaacgtttac aaggaaaaat caagaaatat gatcgtgtgt tatctagaaa
    32281 taaaatggaa tgtatcaagt aaaaaaatca aggagcatcg ctatggagtt acgcttacta
    32341 aaatattttt ggacaattgc agaagaagga acaatctctc aagccgcaga agttctacat
    32401 cttacacaac caaccttaag ccgacaactg aaggagttag aagaggaact aggaacagaa
    32461 ctgtttgtac gcgaaaaccg caaaatgatc ttgacagaag caggttattt tctaaaaaag
    32521 ccgagcagaa gaaattttag atttaacgaa tcaaaccact caagaattcg agaacagaaa
    32581 aaaacaatta ttcagcggcc gatttacgat cggctgtgtg gaagctgata attcgaatac
    32641 attggcacta ttactagaaa agttgatccg tgattatccc agcgttcaat ttcatatcca
    32701 cagcggaaca agtgatgata tcacggagtg actagaaaaa agcctgttag atttagcgat
    32761 tttgctagat ccagtagata cagagaaata cgaaacgatt ttgttaccaa gaaaggaaaa
    32821 atggggattg ctcattttag aaaattctgt tttagcaaaa aaagacgtga taacaaaaga
    32881 agactttgcg aatgtacctc ttttgatctc aaagcgtcca gaaatacagc gaatgtttgc
    32941 tgactggtta ggcataaaaa aggagcagtt gctgatcaca ggaacgtaca atttgatttt
    33001 caatgtattt gcgctaattg agaatcaggt aggtgctgct ttagtcatag aaggtgctgt
    33061 aagtcatctt tcaaagcgag tgacattcgt tccgcttgac ccaatgattg aaacgaactg
    33121 catgctagtc tggaaaaaga atcggatttt atcacctgta gtcaatgaat tgattcaccg
    33181 tttcaaagaa gcttttgaac agtaataagt atctctaatc gaagaaattt caatacaaaa
    33241 gcgagacgta tgacagtctt gcttttgtat tttttcattt ttaaatcaaa aaaaataaag
    33301 agatttacat aacttgttaa ataagcgctt acaaacataa gaagaacgga aatgaatcag
    33361 ctataataac cgttgttctg gattgtaatc attacaaaga aatgaagatt ataactttgg
    33421 aggtgttcga aatgaaagaa aaaaagcaaa aattcgttca aaaaacatgc cctttattca
    33481 atggttgggt atgattctgc tgattgtatt agctttattg acgatcattc cttttttatg
    33541 gatggtttct gcttcattca aagcaaataa tgaagtgttc acgattccga ttcaatggat
    33601 ccctaaaact tggcatttag agaattatct ggttatttgg tctcgtattc ctttagtgac
    33661 attttttaag aatacagctt ttttaagcgt tgtgatcacg ttgattcagt tgtttacttc
    33721 tagttttgct gcttacgggt tttctaaact aaattttaaa gggcgcgata ttttattttt
    33781 agcatatatc ggcacgattg ctgtcccttg gcagtcttat atgatcccgc agttcattat
    33841 gatgagaaaa gtgggtttaa catatacgtt gtggtcgttg gtgctattac aagcgttcaa
    33901 tgcttttggt gtgttcttgt tgaagcaata ctacagtagt atcccagatt ctttatgtga
    33961 gagtgcgcgg atcgatggct tatcagagtg gggaatctat cgcaagatca ttttacctct
    34021 tacaaaaccg gcgattgcca gtttgacgat catcacattc gttaatacat ggaacgatta
    34081 tatggggcca ttcatttatt tgtcttctac ggaaaataaa acaatccagt tagggttgaa
    34141 aatgtttatt gggctatttg atgcagagta cgcattaatc atggctgcct cagttgtttc
    34201 tgttcttcct gttgctttta tatttttagc gatgcaaaaa tattttgtag aagggatcgc
    34261 ttcatcagga atgaaggaat aggaggtgat tatgaaagag cagcgttttg acaaaaatgt
    34321 ttatatggaa ttcttttatt ggttgtatca atcattggca agtagccttt gtttttggtg
    34381 tgtcaatatc ttgtatatag cagctgcgtt ccttttgtcg atagatggaa ggaatgtctt
    34441 ttcctttatt ttttctttac tttttattgg tccaggttgc
//
LOCUS       DAFBQW010000022        29141 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFBQW010000022 DAFBQW010000000
VERSION     DAFBQW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.023"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..680)
                     /locus_tag="LK889_001389"
     CDS             complement(<1..680)
                     /locus_tag="LK889_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5095631.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELR"
     gene            complement(746..2035)
                     /locus_tag="LK889_001390"
     CDS             complement(746..2035)
                     /locus_tag="LK889_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK5095632.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(2122..2826)
                     /locus_tag="LK889_001391"
     CDS             complement(2122..2826)
                     /locus_tag="LK889_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBK5095633.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            3026..3292
                     /locus_tag="LK889_001392"
     CDS             3026..3292
                     /locus_tag="LK889_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5095634.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            3305..3463
                     /locus_tag="LK889_001393"
     CDS             3305..3463
                     /locus_tag="LK889_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095635.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(3460..3690)
                     /locus_tag="LK889_001394"
     CDS             complement(3460..3690)
                     /locus_tag="LK889_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5095636.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            3762..3917
                     /locus_tag="LK889_001395"
     CDS             3762..3917
                     /locus_tag="LK889_001395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095637.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            4074..4229
                     /locus_tag="LK889_001396"
     CDS             4074..4229
                     /locus_tag="LK889_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095638.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(4226..4450)
                     /locus_tag="LK889_001397"
     CDS             complement(4226..4450)
                     /locus_tag="LK889_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5095639.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            4717..5052
                     /locus_tag="LK889_001398"
     CDS             4717..5052
                     /locus_tag="LK889_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095640.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5285..6226
                     /locus_tag="LK889_001399"
     CDS             5285..6226
                     /locus_tag="LK889_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBK5095641.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            6228..7118
                     /locus_tag="LK889_001400"
     CDS             6228..7118
                     /locus_tag="LK889_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBK5095642.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            7161..7961
                     /locus_tag="LK889_001401"
     CDS             7161..7961
                     /locus_tag="LK889_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK5095643.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7976..8827
                     /locus_tag="LK889_001402"
     CDS             7976..8827
                     /locus_tag="LK889_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5095644.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8824..9141
                     /locus_tag="LK889_001403"
     CDS             8824..9141
                     /locus_tag="LK889_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095645.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            9134..9316
                     /locus_tag="LK889_001404"
     CDS             9134..9316
                     /locus_tag="LK889_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095646.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            9318..9788
                     /locus_tag="LK889_001405"
     CDS             9318..9788
                     /locus_tag="LK889_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095647.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            9785..10327
                     /locus_tag="LK889_001406"
     CDS             9785..10327
                     /locus_tag="LK889_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBK5095648.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            10324..10707
                     /locus_tag="LK889_001407"
     CDS             10324..10707
                     /locus_tag="LK889_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095649.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            10704..10934
                     /locus_tag="LK889_001408"
     CDS             10704..10934
                     /locus_tag="LK889_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095650.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            10941..11144
                     /locus_tag="LK889_001409"
     CDS             10941..11144
                     /locus_tag="LK889_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBK5095651.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            11141..11437
                     /locus_tag="LK889_001410"
     CDS             11141..11437
                     /locus_tag="LK889_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095652.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            11514..11927
                     /locus_tag="LK889_001411"
     CDS             11514..11927
                     /locus_tag="LK889_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK5095653.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            11940..12326
                     /locus_tag="LK889_001412"
     CDS             11940..12326
                     /locus_tag="LK889_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095654.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            12818..12890
                     /locus_tag="LK889_001413"
     tRNA            12818..12890
                     /locus_tag="LK889_001413"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12851..12853,aa:Lys,seq:ttt)
     gene            13276..13536
                     /locus_tag="LK889_001414"
     CDS             13276..13536
                     /locus_tag="LK889_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095655.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            13579..13752
                     /locus_tag="LK889_001415"
     CDS             13579..13752
                     /locus_tag="LK889_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095656.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            14141..14974
                     /locus_tag="LK889_001416"
     CDS             14141..14974
                     /locus_tag="LK889_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBK5095657.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            14967..16382
                     /locus_tag="LK889_001417"
     CDS             14967..16382
                     /locus_tag="LK889_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBK5095658.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            16394..17923
                     /locus_tag="LK889_001418"
     CDS             16394..17923
                     /locus_tag="LK889_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5095659.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            17841..18893
                     /locus_tag="LK889_001419"
     CDS             17841..18893
                     /locus_tag="LK889_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBK5095660.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            18895..19212
                     /locus_tag="LK889_001420"
     CDS             18895..19212
                     /locus_tag="LK889_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095661.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            19339..19962
                     /locus_tag="LK889_001421"
     CDS             19339..19962
                     /locus_tag="LK889_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBK5095662.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            19977..20867
                     /locus_tag="LK889_001422"
     CDS             19977..20867
                     /locus_tag="LK889_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBK5095663.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            20890..21105
                     /locus_tag="LK889_001423"
     CDS             20890..21105
                     /locus_tag="LK889_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095664.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            21117..21458
                     /locus_tag="LK889_001424"
     CDS             21117..21458
                     /locus_tag="LK889_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095665.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            21458..21829
                     /locus_tag="LK889_001425"
     CDS             21458..21829
                     /locus_tag="LK889_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095666.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            21822..22217
                     /locus_tag="LK889_001426"
     CDS             21822..22217
                     /locus_tag="LK889_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK5095667.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            22219..22596
                     /locus_tag="LK889_001427"
     CDS             22219..22596
                     /locus_tag="LK889_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095668.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            22597..23205
                     /locus_tag="LK889_001428"
     CDS             22597..23205
                     /locus_tag="LK889_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5095669.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            23205..23546
                     /locus_tag="LK889_001429"
     CDS             23205..23546
                     /locus_tag="LK889_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095670.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            23788..26577
                     /locus_tag="LK889_001430"
     CDS             23788..26577
                     /locus_tag="LK889_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBK5095671.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            26567..27307
                     /locus_tag="LK889_001431"
     CDS             26567..27307
                     /locus_tag="LK889_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5095672.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            27304..>29141
                     /locus_tag="LK889_001432"
     CDS             27304..>29141
                     /locus_tag="LK889_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5095673.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT        10360 a         4251 c         5923 g         8607 t
ORIGIN      
        1 tttcttaatt caccaatgta ttttttgttc gtcaacatat ttttaacact ttgataatct
       61 ctaacgattc catactgaga gcctaaataa taaacagttt ttcttaaact accactagaa
      121 agaaagtagt tgaatatatc tatcacgata ggagcatcat catttattac taatttctta
      181 ttttctatag agtagcctaa cggagttttc ccgcttacta cttcaccttg tgcaatttta
      241 tttgccataa cagctttgat gcgttctgaa tcaatttggg cttccaattc agcaaaactc
      301 attacttgtg caatgaaagt tctgccatac gctgtagttg tatcataata ttgttgagaa
      361 acagcattcc aagaaacatt gtgtttttct aaaatttctt gagtgtttaa ataatgtcgt
      421 agatttctaa accatctatc taattttgta aataaaatta gatctatttt attttctttt
      481 acatcttgta atagtttttg aaattcatca cgatttattt tttgaccaga aataccaccg
      541 tctacatatt cattaactac tttcaaatcc ttgtgttgtt cgatatattc atagagggtt
      601 tctttttgtt ctctaagact atctccatgt ttggcttgtt gttcagttga tactctcata
      661 tataatgcta ctcgtttcat gttatctcct ttcgaatgta tgttcttatc gttgtaaaag
      721 aaaagcccga aggctaatct ttttactaca catctgttaa ttctaatgat cttctgtatt
      781 gttcagcttg agctagttta gtaaaagata cagttccgtt ataattttct aaaacaactt
      841 tttcaatttc atcaagagat actttaaaga attcttttcg ttgattaact ttattgactt
      901 cgttttttct aaaatgcgaa tgtagttgat tttctaatgt tggtgcatct tctgaaaata
      961 tcattgcatg aacatcaaat tcaaaaggta cagaagcgct actaagttcc ttaattctat
     1021 ccataggttc taaccgtcta gtcattccta ttttatagac atcttcaccg aaagatccga
     1081 tattactgat gacataaaca tagccagctc tagtattaag ttctctttgc aatacatctt
     1141 ctttgacagt ttctagttct cttaatttct cttccagttc tttaatttta tcagcataga
     1201 gttctttttc aacatcagca ctagcttttt gcatgtatgc cattaactta gttatctctg
     1261 agttaaactg tcgttcttct ttatcaatgc gtttcttttc acgttctatt tcacgtctta
     1321 ccttttcttc ttcaatcatt tgttctttta ttgctttttg ctgttctttt tcatcgttaa
     1381 ctttaactat gtatgaatag ttgagttcta attcttgaag ttttaattca agtaattctt
     1441 ttttgaattc tgcttcatcg tttttaaaaa gcttgtttat tttatcaaaa gttcttataa
     1501 ttttttctct agttgaatct acgttagaaa tagtaagttt attgagaagt ccagttattt
     1561 ctgattctaa tggtataagc attttctttt taatattatt agcttctcta ttattcaaat
     1621 aagaaggaat attaattgca ccgtctaaaa taaaatcctt ttctttaatc tttaataaag
     1681 aaagtttgtt ttttatttcg gatgaagaag tttcatctat attcggaata attactgatt
     1741 tatagatagt gaccagagat gtgtcatatt tttgattaat ttcatcgtct aaccgctggc
     1801 tttcagcatt aagaatatca attttgtttt ctagcgttga caagttttta ttcaatttac
     1861 ttatgttttt ctctttttca gatataaacg tttccctagc atttaattct tgagaatatt
     1921 cctttacctt actctctaag gcagaagttt tatcttttaa ttttttagct tcagataatt
     1981 tgctactacc cataattatt aaaactattc caactaaaaa caatagtact cccattcaaa
     2041 acgcctcgct tcttttataa aataattgtc aaaatcatat aaacccttac cgaagttgac
     2101 ggctccggca tgggaatttt tttaaaatac aacttttccg ataattctaa tctgttctgg
     2161 cgatacaatt ctatcttcgt ataagtcgtt tatagaacgt aaaatgattg tttgattttc
     2221 gtaatcttta tatatctctt tacatgtaac tccgtcacca cctaattcca caatagcaat
     2281 ttccccatct tctacgtccg gttgttcttt aacgaatacg aattgtccat tcctgataac
     2341 aggttccatt gaatcgccta taataggtac tgcgaagtca gcagcacttg gaactctctc
     2401 aaattcttcc tcttcgacaa ccgtatcacc gtaagtaagt tctgttggat tggcagccga
     2461 agccgaattg tgaacagttg ggattttttc ttttttagga aaatctagga cattttcttg
     2521 tttttgttct tctaatcgat tacttgcaaa ttcataggta gcaactttat tttcgtcgtt
     2581 aagctgatta tatataggaa ggatattata tatctgatta gatatagata aatcaatatt
     2641 catcaaatca tctatatcaa cattgaatat cttagctatc tcatttaagg tttttaattt
     2701 tggtgtatat tttccttttt cccattcgct tattgaggat gaactttttc ttcctagttt
     2761 ttcggctagt tcttgttgat caatattata cttttctcta agaaacttta gattttcagc
     2821 gaacatagta acacctcctt gagttaattg taacaccgtt tcggaaaaaa tgtaacaggt
     2881 ttcttagaaa atattttagt gtttttaaag aagtatttac gcaaaataga aatcgatttc
     2941 aggaaaactg aaatatgcac ttgacttcgg aaaaaccgaa gtatataatt tagtcagaaa
     3001 gttaaagaga ggaggcgaat aaaatatgga taccgcgaag aaagaaaaat atacattgag
     3061 acaattaaga gctttaaagg gttattcaaa agaagaactg tcaaggaaaa gcaaagtgac
     3121 atcaagaaca atatttattt acgaaaacga tgtagataaa atgaggaacg gaaagtacgc
     3181 cactctagaa aaaattgcta aggcattggg tgttagagtt tccgatattt ttttagaccc
     3241 tgattcggaa aaaccgaaat caataacagg tgaaacaaaa caagtcagct aggaggtgtg
     3301 aatagtggaa gagaagaata taaaagtacc gactcttgaa gaatatacaa aaattgttgt
     3361 cgaaacagac gagcagaacc cgaaaacaat cgcagttatt acggcgaatg atattgaatc
     3421 atgcgaaggt tttcgagttc gaatgacccc tagatatgat taatgttctt tatcacgtgg
     3481 aggtatagga tcgtttccaa aactatcttt tgattgtatt tgtccattct ttttttggac
     3541 aataaactct gattgctgtt ttttagaaat ctttttacca tactcaacag catctgattt
     3601 attatcaaat agttttgtat cttcggaatt accttcacca cgtacagccc atttgccatt
     3661 tctaggagat acccattgat ttttacccat aacaattcac ctcgctttca agttaattct
     3721 attaagaggt gaaaagaaaa caaactgatg gaggtgagat tgtgagtaac aagccaaaaa
     3781 tcactttgaa tggcacacct aatcttcaaa gattagcaga tgaaatggct agaatacgtt
     3841 ctagacattg gggagttact gtggtggcaa ccataattga acaaaaagaa aagcccacac
     3901 agccggcaag caagtagtgg gcaccataga acaataatat tatatcacac actcgccact
     3961 tccgaaaaga ggagttgctg ctctcctttc aagtttgcca aagtccacgt ttcttcattt
     4021 tggcaactcc tcttcttgga ggtggtgacg gcgagagacg aaggagggat gacatgaagg
     4081 aagaatcaaa aatagggatt ttgaatgaga acaattacac aagattaatt attgaaacag
     4141 aagaagggga aaaagtagca gaagttacat tgaccgatgc aactcctgct gacggttatc
     4201 gtattcgatt aactcctaaa tatgattaac cttttggtgg gtgtggatcg tttccgtgac
     4261 tatcacggct ttgaattttt ccatctctgc catgaataaa tagctcagat ccttgatttt
     4321 tagaaatctc tctagcgatt tttactgctt ctgattttgt attagtatgt gcagtcgctt
     4381 tagaatttcc agcacctttg acattccaac caccattgga attaggtaca acatgttgat
     4441 tttttcccat agttttcacc tccatttcta tagaaatatt ttatcaaata gaaatggaga
     4501 aattcaataa acgaaaggag ggatcaaatg acattacaag agcatcaaca cttgatgcga
     4561 aaactaaatc aggaatacca cgaggaaatg aaacctagat tgctagggga acaatggcgt
     4621 gaacgccaga agaagtggtt agaaataaaa aagtgactca gccgtccaaa gcaatgagtc
     4681 acaaaccaaa aattatacct aaggagattt tatcacatga ataagaaaat tgaaaatctt
     4741 atcgaagaat tgaaacgtga atgccaaaag caaggtgttt ctattatttg tacagcccaa
     4801 aaagaagggg aacttaaaag tctcgtttat ggtgagacaa cagaaatttt actttgtctt
     4861 gctatgcaag aagaacattt agatgaaaat ctcccattgt cagcacatat tatgcgtaga
     4921 attgctgtgg atgcttacga acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat
     4981 acttttgtta ttaataacaa ggaagattta gctgatgtga tgactcgtat tctcaagggg
     5041 gaatttcaat gatgaatgaa aaaacaattg atctactcat tattttattt ttcacgttag
     5101 tgattgtagc gaaaagttat gtaatctttg gcttgttatt cgcaattacc atgcttgtgc
     5161 tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg agttagcaaa tcttgacaac
     5221 tatttaactg atcctgatta tgctaaacca ccatatgaag cgccaattga tgaggaggat
     5281 gaagatgagt aaatctacct tagaaatgag ccatcaagaa tggctcgaag accgcaaaag
     5341 aggtattggt ggctcagatg ttgcaaccgt acttggatta aacaaataca aatctcctta
     5401 tcaattgtgg cttgagaaaa cgggtcaaat tgaattgaaa gatttagaaa gcgaaccagc
     5461 ttattggggt aatgttttag aagaagtggt tgctaaagag tttcaggaac gtacaggcaa
     5521 aaaagtacgc agaagaaacc aagtatttga acatccatta catccatttt taagagcaaa
     5581 tattgatcgg gaagtagtgg gagaaaatgc cattctggaa tgtaaaacag ccaatcaatt
     5641 tctcggaaaa gagtgggaag gtgaagaggt accgcttagc tatctctgtc aagttcagca
     5701 ttacatgaac gttttaaaca aagactattg ttatatcgct gttttgatcg gtggacaaaa
     5761 attcatttgg aagcggattg aacgagatca agagctgatc gatacaatca ctgaacaatt
     5821 agtagagttt tgggaaacga acgttcttgg aggtatcgag cctgtaattg acggaagtgg
     5881 agcgactgct gacttcttaa aagaaaaata tgcagatgta gaagaaaatc aaacagctct
     5941 accatcacgt tttgatgaac ttatcgagca aaaaagagaa ctcaagcgga cgaaaaaaga
     6001 aattgaatca tctattcgtc aagtagacaa cgagatcatc agtgagctag gtaaacgtga
     6061 agccagtatc ggtatcacac aaagaaacat tatcagctgg aaacttgttc gtacaagacg
     6121 gatgaacacg aagaaactag cagagaaata tccagatgtc gcaaatgatg aagagattta
     6181 taacgttact gaatcaagaa ggctaaccga aaaggagatc aaataatatg gcaacaaatg
     6241 aatcgttaaa aaatcaattg gcagaaaagc cacagaaaca agttgcacca ggacagttag
     6301 ggcttaaagc tctaatgaat acaccaacaa tgagaaagaa atttgaagaa gtacttcatg
     6361 acaatgctaa tgcttttatg tcgaatgtta tgactcttgt atctaatgac agttatcttg
     6421 cagatagtga accgatgtct atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg
     6481 ggctagataa gaatttaggt tatgcatatt tagttccatt caatagtaaa aacaagcaaa
     6541 caggaaaatg ggaaaagaaa gctcaattca tgcttggcta taaaggatat atccaattag
     6601 cccaacgatc aggtaaatac aaagcattaa atgtgatcga agtttacgaa ggagaactaa
     6661 aaagctggaa ccgactgaca gaagagtttg agtttgatcc aaatggtaga acgtctgatg
     6721 aagtcattgg atatgttggc tatttcgagt tactgaatgg attcaaaaaa actgtctatt
     6781 ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa taaagatcgc gataagacaa
     6841 agttaagtgg tgtgtgggca tctgattaca atgcaatggc acgaaaaact gttttgagaa
     6901 atcttctttc taaatgggga atcttgtcca tcgaaatgca agaagccacc acatcggatg
     6961 agagagtcca aagggttcaa gaagacggca gcattattgc tgaaacagaa gttgaagaag
     7021 atattcctga aagaaaagaa gcagaggtta tatctgaaga aaatgaagat gtacaaactg
     7081 gattatttga tgcatctaat ccgccgttaa acaaataatg agggagcttt ctcccttgct
     7141 tttctagagg agaaatacga atgaacacag gatatgtcaa attacatcga aaagtgatga
     7201 attcattcgt ttggaccaat ccttatatgt ataaattatg gagcttgtgt ttgatgaaag
     7261 cgagtcatga gaatcgcaag atacttttta atggaaaaga gatacaagtg aacagcggag
     7321 aattcgtcac agggcgcgat gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac
     7381 atcaagtgaa cagcggttct gtatggagat ggctaaaaca atttgaaaaa aacggaatgt
     7441 tgaacatcaa atcaactacg aaatacagcg ttatatcaat aaaaaactgg tctttgtacc
     7501 aaagcagtga acaacaaatg aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa
     7561 acaagaatga aaagaattat aagaatgaaa agaataataa aacatcatcg tcgcaaccac
     7621 gcaaaaagcg tgtttacgac accgactcag tttactacat tctcgcggag gagttattca
     7681 aacagatttg tcagaatcag gaaatcaaaa agccgaatct gcaaagttgg gcagataaca
     7741 ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca ggtacgggga atgattgaat
     7801 ggagtcagca caatgtgttt tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa
     7861 aatacgacac aatggcagcg caagcaaatc gtgattataa aacaaaacaa actaaaacgc
     7921 ttgaatacga gaaatttagc acagatgagt tgcctatttg agaggaggcg taggcatgga
     7981 aactgttggg gaaatcatgg aaaagctgat acagaaagtg cttgtccaac gtggcgaatg
     8041 tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag aaccctgatg gctcagaacg
     8101 ttgtaagcca acctgtatgc aatgtgggta taaagcattg cgagttcagg aagacttaca
     8161 aacagaacga atttacaacg agagcctgaa agcaagagca atcaattttt tcaaaggtgg
     8221 ttctgttgtg cctaatcaag cgttgtttga ttgcacattg cagaattatc aaattgtcga
     8281 tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt gttaattcag tcttgttagg
     8341 aaatccaagt caccttgttt taactggaaa acaaggaaca ggtaaaagtc acctagcgat
     8401 ggcagcggct tgggaagtct tgaagcgatc aaactacgat aaaaaaatct tatttatcgg
     8461 gttacaggaa atgctggatc aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac
     8521 gattgaggga tcgttgattg cagatatcaa aacagcggat ttagtcatca ttgatgatat
     8581 cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg tttggcataa acacgctaaa
     8641 ttcgttcttg gatgcacgac agaacctagc aacgattatc acaacgaact tgcttggtga
     8701 agaactgaaa aaagcttacg gtacgagaac aatatcaaga atgtttgtca actctgatgg
     8761 atttacgatg gtattttctc aaacagcaga caagcgcata aaaccagtca aaggagcgat
     8821 cgcatgaatt tgactaaaca agaaaaaaga atctgtcgtc agataatgtt gcaaattata
     8881 ggtgatcgtg acatggacga attatctcaa caagagagtc gaatggttcg gaagataagt
     8941 aagctaatcg gtgcagatac gatggatcga tccaagccat tacccaaact gaattttgaa
     9001 tcatttacgt ttgagaaata tagccatctg agagaggtag gctatagtgt aaatgctatt
     9061 cgaaaagcac ttggggtagg aagtagtgta tgggcaaaat ggcggaaaga aaatgtaaat
     9121 gaggtaaatc aaaatgatta agctaatagg caaagagacg ctcaaggttt actatgtagc
     9181 ggaaacaaag gcagaagtca gccgttggct attgaaaatg tttaataaga ctagagccaa
     9241 tgcttcagta agtcagaaag caagtgtatc agaaacaggc atgcctgaag caatgtggat
     9301 ttcaaggagg aactgagatg gcaatgatac cgaagtttag agcgtgggat agagaaaaag
     9361 acagaatgat ttatcctagc acgacaggag tttgttttga aatgaatgat gatggtataa
     9421 gtgtgcttga tattgatgta gattatccca atgactatgg ttttcccaaa atagcttcaa
     9481 tcctcatgca atctaccact ctgaaggata agaatggtgt ggagatattt gagggggata
     9541 tagcgcttat cactcataaa tcaacgaact acgccgatac ttattggcac agttacgtag
     9601 aagtttttag agcaaaaaac ggagcctaca gaattcgcgg tgaacacatc tacgaaacag
     9661 aattatatag taatagaaaa caattgacgg tagcggggaa tgtttataat tcaccagaac
     9721 ttttgaagag agatcgattt atgactacta ccgaaaatcc agagttattg aaggaggaac
     9781 agcgatgaat aaacaggaat tgattgataa atatactgca gaaatatcta gattaagacc
     9841 ttattgccct aatagacatt taattagcga tcaacttaaa tatggtcttt ataaagaaat
     9901 cctagaagat ttaaaacaac tagacgaacc gcagaaacca gcggtgccta agtttgtggc
     9961 ggaatggata gaatacgcta aaaagaaagg cgatagtcta gctatttcat tcaagccgtg
    10021 gaacctctac ggtgttgagt atagcaagac tgatagatgg attgaagata atcaaggaac
    10081 gtttgctcgt gcttggctaa ttggctacga ggtcgagaaa gagccgttgt attatgtgaa
    10141 gttgccagtt gtgtatttta atcattggga tttagagacg tatctaatga aagatgatag
    10201 aggaaatata acaattgcag acaacaacga ttttgatgat atgaaattta cggaatcaga
    10261 aataaaagca attgatgaaa gatactggcc atttgctgtg ccagtggaag aggtggcgga
    10321 aggatgagca atcttaaagt tggagatatt gttttttaca aaggtgacga aggcatcatc
    10381 cgagaagtta tgacggatgg aaaagaaaat ttttataggg tagatgtcag cagaaaatct
    10441 atactctacc tgtatgaaga cgaattagat ttaggtactc aaaaactcaa cgacaaccag
    10501 aaaatggtgt ttgattatct taaatcaaac gttgaacaag gtggcaaatc agttatgtat
    10561 tcgatattcc ttctaggtga ttgggactct cgaattggag cagcaagaaa cgttgatata
    10621 gcttactgtg gattaaatgg taagcaggaa gcagaagtcc tatcagcatt cgctcaatgg
    10681 ggattagaac aggaggaagc ggaatgaaat acgaaatacc actaagtgaa gcagacgtcc
    10741 agtcaattat taacggtcgg gaggttaata aaaaacttcc tgatggtact gaattagtca
    10801 tcagacaaag ttatttgaaa gatatggcag ctccagtatt aattgatcgt tttaacgtga
    10861 ctgattctgt ggtagagaac catttaaaag aatttcgatc aagtatagac gacactttca
    10921 gattagggag ttgattgaca atgaacaaca gacatcgcag aataacaaaa ctaagaaaac
    10981 aggaactgaa tgtactaaag acaaagtttg aaaaagaata tggaatttca gcagaagaaa
    11041 catataaagt ggcaagtcag tgtgttgctg atgcgagtaa tgctattcgt aagtttggga
    11101 tttcgatatt aaatgatgat cgtaaatggg aggaaatgag atgaaactaa aagacggatt
    11161 ttacgctagt agtcacggca tcggcggttt aatgctagat atgccgacaa agaatcctaa
    11221 aacacgtaag aaaccaaaag tcaaagtcgg tgacatggtt cgctgcgaag cagaagggtt
    11281 catctatcca tttcgtggat atgtagaaca cgtctataat cactcagcaa tcattcgtat
    11341 tgaaaacacg atggaatgtg ataaatggac agctaaaagc aaagagaatt tagcagtggt
    11401 gagattggtg gatattgaac taatcaatga caaataaaaa agccggatcg ctccgactga
    11461 ttcaataaat tccacaagtt tattatatca cataaaaggg gcggtttgac ttgatgcaat
    11521 tgttacgaga ggtagctttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
    11581 ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg ccgattatta
    11641 cggacatgcc gaaggcaccg aagcacggca ataaggcaga agacgcgatc attcagatga
    11701 tggatataga agcagagaga gacgcgattt tagcggcttt gatggcgctt agtctgatta
    11761 gtcgtcagat actctactac agcttttgtg tgccagatag cttctcaaac tacaggatta
    11821 gccgtgaagt gggttattca gaaagaagca tacaacggat gaagtcggaa gctctgatag
    11881 agtttgcaga agcatataaa catgggaaaa taattgctta taaataggag gactatataa
    11941 tgtggaaaga ttatgtatca ttgaaagagt tgaaaaaaga tcttattttc aaaaaaatcg
    12001 tagaatggtc agagagtgaa ttaattttag aagatggaac caaaatggaa gttgtatgta
    12061 gcgaatcaga ttgctgcgca tgggccgaag gtgaattcaa aaatgtaaag cttgatgcag
    12121 tgattacaga tataaagatt tttgataaag gtaaccgtct ctataatggc gatggacata
    12181 gttcttatgc cgaagtcgtt gtttatcata atcgaaatga gattgctaaa gcggaatgta
    12241 cagcaaacga tgggaatgga ggctactatt acagtgtctg tgctttaaaa gtcaaagaaa
    12301 aactttgtgt agtgactgat gcataaaaaa tggcggtttt ttggcggaat gatggcggtt
    12361 tttagctatt taccagtgat attatggtag tgtcgaaaga ttagtgatag gtctgagaca
    12421 aaataaaatg taagggagga aatctccctc atcgtttaat taagcttcga tagacagcag
    12481 cggaaatatt aagaataagg atgtgaattt taactccttc taaattgttc ttattatcta
    12541 tcatccgttg ctgtctattg ttattatgtc actgtggcgg aaagggtaga cgcttaaaaa
    12601 taaggtcaat acgtcgaggg atagccttaa cgttttatga tttgaccatg caaggttcga
    12661 ttccttgcca gcgacttaga cgaaaggtag cttaaatatt aaccacatca acatcgtcag
    12721 cgagttgata aaggtaacta gcagactcta ggatttaagc cagtcttacc gattagtctg
    12781 ttatagtagc aagcttgcta gaggtagctc ctctggttga cgtgcagctc agttggtaga
    12841 gcgcctgact tttaatcagg aggtcgccgg ttcgaatccg gtcacgttaa taaagaacct
    12901 acggaaacaa tcaatcttat cggatgccga ttggttggct gaattaagca gtgtgtctgt
    12961 gaatcagtta ataggcataa aatactagca cagacgtgtg ccactctcag gtgtaggtta
    13021 ggagagaaac attagcaacc gagggttgga aatgggcgct caaagtacac gagcaaggcg
    13081 aggtcgatag taatcgatgg aatcggtgta ggttgctatt acataattga ttgggttaga
    13141 ttgagttttg ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac
    13201 gtccctgcct gtgcattaca tattagatca ctcgttgagt ggtcttttta ttttagaaaa
    13261 gagaggattt tgaaaatgaa tcatgagaag tttatcgaaa aatgcaaggc tattgtacgt
    13321 gaaagaattg agaatgagat tgctgaccta agtggagcag tacctgagtt tagtgttttc
    13381 atagtctggt catgcaaaac actacagaat agcaaagcat tagtcagcgc tagcttaaaa
    13441 ggagcaccgt attttgaaat tacgttgaac ggggacaaag gtgaaatcta tgtagatact
    13501 tatctcaaaa aatcaaatga atgtatcaaa gtctagcagg tgctaggctt tttctttaca
    13561 taaaggagga atgatctcat gaaacaatat accgctaaag atttcgagga aatgaagcga
    13621 ttaaagaagg actatgaaga agttgatatg gagctaactg ttggagtcat tcaacgaaga
    13681 ctgcgggtcg gattagagac agcaaaggct atttacaatg atctaaatgc tattgaagag
    13741 aagaatggct aatgaggaac tactggtata tatcgctaac taatgaatat cctcgaacca
    13801 ttgatgattg ttcagtgcgt gttgtgcgtt ctgtacaaat caaagggaag tactctattg
    13861 tcgaaatggg gagagaagca gaaccatgtg agattgatgc gtgcaagctc gtttattgcg
    13921 gtcatggttt ctatgatgaa ccaaacattc aaaataatat taacaagaat ttgagggatt
    13981 agaatgcaag aaatggctta aaaatctcca atcaggacaa atcaagttag acaaagtttc
    14041 cgatttaaag atattaattg aagcagatct aatgttgaaa gatattgaaa attagaaaac
    14101 aaaactcaac ctaagaagat tgcgaggtgg tgtgtattga atggcaagac aacgtgatcc
    14161 aagacgtgat gaagccaaaa gaatttggtt agaatccaac ggagaaaagc agttaaagga
    14221 aattgcatct gaattaaatg tttcagattc tcaggttaga aaatggaaat cgcaagacaa
    14281 atggagcgct gaattgaaaa gtaacgttac caatggcaaa agtaacgtta ctaatcaagg
    14341 tggcgctcct attggtaatc aaaatgctaa aggtaacaaa ggaaatagcc gagcttctcc
    14401 gccagtgggt aataaaaacg ctttgaaaac aggcgagtat gaaaccatat tttttgagac
    14461 actaagtgat gaagagaagg acatctattc tagtctgaat gataatcctt cttttgtttt
    14521 gtctgaagaa atacgtctac ttaagataag acaatttcgt atgatgaaga gaatccaaca
    14581 agctgaagct ggactaaatg atgaagaagt cgaacgattg cagcagctaa gaaagattaa
    14641 aaatccgatt gaaaaaaatg gtaaaaagct agaaatcaag cgtgaggtta tgcaagatgt
    14701 gcagattagc agaaaaaaac atcgcaaaat tgatgatatt ctttcaattg aagattcatt
    14761 gactcggatt agcaaccagt tagctaaagc catcaagcaa atgaatgaac tttatatgaa
    14821 tgaatacaga actgatttaa ttaaagctca gactgataag atccaagctg agacaaatga
    14881 aattggcgga aataattcag gtgaagaaat agaagaatgg aaacaggcag ttttaaatgc
    14941 cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt tattcctttt gctgatattg
    15001 gttctgccat tgattattac tatgataaac cagtagcttt ttgccaagat attttgcatt
    15061 tgaatcctga tgaatggcaa gaaaatgttt taaatgattt agctgaattt tcaaaggttt
    15121 ctgttcgttc tggtcaagga gttggaaaaa cagcattaga agcaggagca atactttggt
    15181 tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc tccgacaatg aagcaacttt
    15241 acgatgtact ttgggcagag gtagctaaat ggttaaatga tagcttgatc aaaaacttac
    15301 tgaagtggac aaagaccaaa atttatatgg ttggtgattc agagcgttgg tttgctacgg
    15361 ctagaacagc gactaaacca gaaaatatgc aaggttttca cgaggaccat atgttgattg
    15421 tggtagatga agcttctggt gtgtctgatc caattatgga agctattctt ggtacgctat
    15481 caggttttga taataagctg ttgatgtgtg gaaaccccaa taatattgaa ggtgtttttt
    15541 acgattccca caattcagac cgtgataaat acagagttca taaagtatca agctatgata
    15601 gtaaacgtac aaacaaagac aatatagaaa tgattcttaa aaaatatgga aaagaaagtg
    15661 atgttgctcg tgtccgtatt tttggagaat ttcccaaagg tgcgttggat tcatttatca
    15721 gtcttgaaac ggttgaattg gctacagaaa aacaaattag tgattcttta gtcaataaaa
    15781 caacggttgc tcatattggt gttgacgtag ctcgatatgg tgatgattct acgattctct
    15841 ttcctagaat tgctaccagg gcattggagt atgagaagta ttcaaaacgt agcaccatgg
    15901 aaacaacagg atatgtcatc aacatggcca agaatctaat gagtcaatat ccgagtattg
    15961 ataaagtgat gattaaagtc gatgacactg gtgtcggagg tggtgtaacc gaccgcctag
    16021 aagaacttat agaagacaaa cattatcctt ttgaggtgtt tggagtgaat aacggttcaa
    16081 catcagaaga cgatttttac gataatttag gtactcaact atggggaaac atcaaggaaa
    16141 tgttagaaga aaatatgaca gcaaatctta acggagaaca gcctgttatt gaattgcctt
    16201 ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt caaaatgaca agtagaagtc
    16261 gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa tattggtagt cccgatattg
    16321 ctgacgcact ggctttagcg ttttatgagc caccaagtca ctatcaattt attcaatttt
    16381 aggaggtgag cttttgatta gtacagtatt atctttagag agatataaga agctacgtgt
    16441 taaatatgca acacaaattg aagatggaat gtttgatccg aatggtttta tagaagatat
    16501 gaaaccattt tttgctgatc gcgaaagaaa atatctagct tatactagcg aaaagaatga
    16561 aatagataat agaccaaagc ctaacactga tattgtaaaa gttaataata aacttcatgc
    16621 tggtatgtat tcaattgtcg tagaccaagc agtcaatcat tttactggta tacctatcaa
    16681 atgggattac gatgtatcgg aacaaaaaag aacactcatt caaagattaa aagataaatt
    16741 cttaaaaaat gatatagaac ttccgacagt tccagaagct tttaacaaat taacaagcaa
    16801 tcttgattcc atgagattcg caatgcttga ttctgaaact gcaacttttc aaggagcttg
    16861 tggagtagct ttccgattgt tagagcctgt ggaagaagat gatggttgga agttaagagc
    16921 aagtaatatt gaaccttgga gagcagaaag atatggaaat gccggaatct atatcaaaga
    16981 aaagtatgac tcttaccaga aaaaattttt tcaagaaatg aaagttatga caagaaataa
    17041 aattctgaca tatgcttgct atggtgattt gaattttgtt acaagcggaa cgttcaaaaa
    17101 aattgatgag accgataacc ctttggggac gattaccttg gcagaattta aaaataatac
    17161 gaatcgttat tgtgattttg aagtagctga ggaaattgga gatgcaattg atcgtgcttt
    17221 gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg tacatgcttg ttactggcac
    17281 aacaatgagc aaaggcactg ctaaagaaat gatgaatcag ctagggatta tcaacttaaa
    17341 agaccctact gcaaaagctg aatatgtcac aaaaaattta gcaaaagatt tcaatgaata
    17401 tcatatggat ctattgaaaa aacagtttta caccatttgt aaagcaatcg attttaacga
    17461 tgaggtgttt aaatcaaaca gttctggaga agctcgcaag tggcaaatca ttagcctaga
    17521 agcgaaaaca aatactaaag agcaatattt tagagaagga ttgaaagaat gcgcagaaac
    17581 aattgctgct tttcttaagt ttcatgacaa agtagaaatt gagcctgaaa aaattatttt
    17641 tactttctct agatctttgc caactgattt aagttattta gctgaagctt tacctaaact
    17701 tgcaccatac gtatccaaac gaactattca gagccaaatt ccatttgtaa cagatgttga
    17761 ttatgaaaat gaaatgatgg aattggaaag tggaagtgct tatccagata gtgaatacaa
    17821 ttttggcgga ggtggcaata gtgacgatag aaacgaagta ttggaccaaa cgtcgagaac
    17881 tggaggatca agcaaggctc aaacaagaaa atcagacact taaaaaatta actagtgtat
    17941 ttcctgaagc actgaaagag atacaagcaa aactattatc acaagctgac ttacacaata
    18001 tcacttatcc agaaatgatg gagttttata gtacaagtaa tcagaaaaaa tatcgtgaat
    18061 atgtggaaaa aaattataag tcattaaaaa tgtatgatgc aaaatacaaa gagtttatcg
    18121 atgaattttt tccaccattt gactatgcaa aagtcaatcg cttattacaa atacgatcag
    18181 atgtatttaa aattcttgca gaatatgcga tggatgcaga tgtgaatcaa tatttttctg
    18241 atcgcttaga ggaaattctt caaagaacat attcttctaa tgctaatgtt tttgttcaac
    18301 ttttaaacgt tgatatacca aattatttac cagaaaatga aattaaacat tatttgaact
    18361 atccatggtc aggaaagaca ttttcaagaa gactttgggg aaatatttca tcgcttgaac
    18421 agaaactctc taatgctatt gtaaaaagtg ttgctagtgg agaaggcgtt atacacgcat
    18481 taaacaccat gagattagac tcagaaattt gtgacatgtt taagttagaa gaatcaaagt
    18541 ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc aaaatttgca caagatggta
    18601 ttgaaaaatc atatttagaa acaggtattg aggaatacaa cgtattgact gcaaaagatg
    18661 agagagtttg ccggatttgt ggaggaaagg caagtaagaa tccctataaa ctgaaagatg
    18721 ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg tagatgtacg gatgttccta
    18781 atttaccaaa attaggaaag gatattgatg aagaatatga ccgtttattt ggcgatttat
    18841 tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag tagaatgaaa
    18901 gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga agcctacaaa
    18961 acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa tggcaattat
    19021 gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc agttgataag
    19081 aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt aaaagaaaca
    19141 gttgattggt atgaaaagat gggatgtatt gttgttagaa ctgactacaa agaaggaaag
    19201 tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc aatactgggc
    19261 ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg tgaaaacgaa
    19321 tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat taatgccaat gaatttgcaa
    19381 tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc ttttgtggaa
    19441 tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc cgacaaacaa
    19501 ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat caaggaatat
    19561 gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact acaagctgaa
    19621 tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt tgcagaacaa
    19681 attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt tatgctaaat
    19741 aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt tacaagtgtc
    19801 ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat gaagtctgca
    19861 tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac attaacaaat
    19921 ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga aaaattatga
    19981 aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt aatgctatgc
    20041 aagtaccaaa tacacctttt ttaagctatt tatttggtgc tggaaaaaca gagccagcaa
    20101 actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct tctgaaaaac
    20161 ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac aactatactg
    20221 aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat gtgaatggag
    20281 tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa attgatttga
    20341 accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt cgtcgaatga
    20401 atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact gcgggtgcag
    20461 taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt tatatgggag
    20521 aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag ttagatggaa
    20581 aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa attggaaata
    20641 tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca aaaggaacaa
    20701 ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg agagcagaag
    20761 aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac tctcttgaat
    20821 acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag ggagttagta
    20881 ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa aaaagatgaa
    20941 gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca tcctaaaact
    21001 cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga tgataaagaa
    21061 gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt gatctgatgg
    21121 atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca gatttgagtg
    21181 aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca tacaacactt
    21241 gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg ttgtataaaa
    21301 taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat acaacgatta
    21361 gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt gatttgatac
    21421 gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag atattttaga
    21481 aactacttat gaagatagtt gtattattga aagactgacg gacattgaag attctaatac
    21541 aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt gcgctctttc
    21601 acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa atgtttcaac
    21661 tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag atcgaataac
    21721 aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc ctttttacta
    21781 tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat ctcaaatatg
    21841 aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt gctcaaaaag
    21901 aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct aaaccattaa
    21961 ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct agaaaactat
    22021 caagcggtcg aattgttgga gcagttataa acaatgaaca ttattttaaa tatgtagaag
    22081 aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta actcgtgcaa
    22141 cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca ataaaaatcg
    22201 ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag caaattaaaa
    22261 gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac taaagatttt
    22321 tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga tattcaaaat
    22381 gtttctttct taattgatat tgcattgatt gataataaac ctaatagaaa tttaattaat
    22441 gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga gcaaatatta
    22501 tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg ttttacatta
    22561 gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag aagtaaaacc
    22621 ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac agggaatttt
    22681 ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc aacagctaat
    22741 tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac agcttttggt
    22801 ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc tacaattact
    22861 ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc gcaagatggt
    22921 gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt cttttataaa
    22981 ggacagtttg ctctagattc agatgaaaca tcaacgattg aaggaagtaa agtaagtttt
    23041 actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata tatgtattca
    23101 gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt tacaaaagta
    23161 gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca aaatatcaaa
    23221 caacgattaa attaacgaaa aaagatgaag aaggtaaata tgagcaagta caatttaaat
    23281 ctgctgaatt tttaccagga acagttgtag aagatgctgc aggagttatg gaagaaatgc
    23341 aaacagcgac tgataaacaa tcagttaaaa aagctttaag tcgtgcttat tcatttattg
    23401 cagatacttt atttgaggga caatttactg gtgaagatta ttgcaaagga attgatgctc
    23461 gtgagattgc ctcattgaca ggaaaattat tgaagtctgt tactgcaggt tttgatgaaa
    23521 cttatacaga gacgaaaaaa aagtaagtga ggcgctcaag tcaccttcat ttaagtattc
    23581 gattacttac cgagaattag atataaagac gcaattactc gaagcaggtt ggacgttacc
    23641 agaaattgaa cgtactgact tggatgagtt aatgcgtctt tttgctttta gagatgcggt
    23701 aaaagagcat gaagatgttg agtactatga taatttcact caattttagg aggtgatact
    23761 ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt tactgccggt
    23821 atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag aaaaagtaga
    23881 aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt tctagctagc
    23941 ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat aagtggagcg
    24001 gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat gggatttagt
    24061 aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg tattcaagga
    24121 cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc ttctacaggt
    24181 gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct tggtttcggt
    24241 ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt ctcaaacgga
    24301 aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc tactctttct
    24361 gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt gtctcaaggt
    24421 aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa aggtggcgga
    24481 ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa aacctctata
    24541 caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg attaaataaa
    24601 gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt gggcagttca
    24661 atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc atttttaagt
    24721 aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat gccctttctt
    24781 gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat accagcaata
    24841 ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc attaaagatt
    24901 atgggggcaa tagctgctgc taattcattt gttttaatta ttggtgcagt cgttggtgca
    24961 atagctgttt tcggttactt tatggcaacc aatgaagagt ttagaaacaa ggttatatct
    25021 atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa ggactgggga
    25081 attgatactt ggaattctgc taaagaaatg gcatcaaatg cagttgaggg tgtcaaagat
    25141 gcttggtcag gaacaaagga atggttttct aacacctgga aaggtattaa ggatggggcc
    25201 acaggtttat ttgataaaac agtagagact tcaagaaatg cagtcgatag tgtaaaaaat
    25261 gcatggtcta atatgaagaa atggttttct gatacttggc aaagtataaa agattcagcc
    25321 agagaaaaat ggaatgaaat taaaggttct attatgtcgg ttgctggtcc attgattaca
    25381 ggtataaaga atgcattttt acatgttact ttttatctag agaccttatg gaataattta
    25441 gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc gccggttttg
    25501 tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaataatat gattgctgtt
    25561 tggaataata ttaaagaaag tgccataaat atttgggaat ctataaagaa tatatttgta
    25621 agttatttta caaatattta ttttgctgca ctcaatattt ggacagggtt caagcttaca
    25681 ttgataaata tttggaatga agtggtaaat caagctaaat cgatttggtt aaacataaag
    25741 tattttttta ttaatctttg gattgatata aaatattttg caattcagaa atggattgaa
    25801 ttgaagtttt ctattattca aacttggatc gatttaaaat ataatgccat tactacttgg
    25861 aataatatta aacagttttt caaagatact tggaaaaata ttaaagatac ggcatacaat
    25921 acgtggattt ctataaaaaa taccatgatt aatacatgga ataacattaa ggactctttc
    25981 tggaatattg ttactggaat tgttaattct gctgaaaatg catggacaaa tctaaaaaat
    26041 ggtgtttcaa atgctattaa tcgggtaaaa gaaatcttta attcgttaag ggaagtcaat
    26101 ttatttgaaa ttggcaagaa catcattgat ggacttataa atggtgtaaa agaaaagtgg
    26161 aatgctttga aagatactat caaaggtatt gcaggtaata tcaaaaattg gattaaagga
    26221 gctttagata ttcattctcc atcaagatgg atgcgtgata tggttggtaa aaatattgtt
    26281 caaggtatta tcgttggtat tgataaagag caaagtaagt tagatcaaac catgacaaat
    26341 ttagtaaaaa caccgtctgt tcaacctgta gtaaatggaa taagtcacct accaattaca
    26401 caaacaaagc aaaatatatc tgctagtgaa acaaaagaaa ttcatttaca tttaaatgtt
    26461 tatggagatt tacctgattc aatggttcgg aagattacca caaaaattaa aacagaattg
    26521 acaagacaaa tgaaacgaga tgctggtgca gtaggaggaa cattatatgc aacttaagag
    26581 aggacaattt tttattaatc aacattattc ttctgaattt aatgtgtata ttcaaaacag
    26641 gcctgcttct gtttcagcta gtcgagtaat tgaattgaga gagcgagaag gaaatgattc
    26701 tatcattatt gataaaggct attacagaaa tgtgactaga aagattgagt gttactataa
    26761 agcaccttca attgatgtgg tgcaggagtg ggaagatcga atcactgaat ggttagatat
    26821 gtcgtcttat agtgatttta ttctttacta tgatgaacaa tatatttatc aagctgtagt
    26881 aatagaagga cctgaattta aaggaacaag aaaaacagga aatattgtcc cttttgaatt
    26941 tacagtgagc attcgaccat ttaaggaaaa ttacaatggt agatttacta ttcgacaaac
    27001 agaaactttt gaaatttata acccagagaa gtattcttca aaaccgctta ttaaattgag
    27061 tggttctgga gatgcttctt tttatattaa taaggacaaa tatgatttga aatcgttgga
    27121 cagagaatta tttatagatt ctaaattaga agaggcttac cgaaaattag atggtaattt
    27181 agaacatcag gatcaagtca ctttattttt agattttcca tttttatatc ctggaaaaaa
    27241 tgaaattaaa tggacgaaaa atattcattc atttgaaata atgcctaggt ggtggagaaa
    27301 agtatgaaac caagaatata tagtcctact gaaacagatt tctcaacgaa tggcttagga
    27361 attttaaagg attgtacaag atgtgaaata tatgaagtag ctaatggaaa atatgaatta
    27421 gagttggatt atcctttagg aactagattt gatgaatatt ttgaaaatga ctatcaaata
    27481 aaagcaaagc caaacgatca agaagagtat catatctttt ttattgatga taaagatata
    27541 gatacttttt taaatacagt aactatttat gctcagagtc gtacaaatcg acttggaaga
    27601 cgggtagtca ctcttgcgga agtagactct aaaactggtc aagaagcgat atcaattatt
    27661 gaaaccaaaa tggataaaaa atctgacata cgactttatt ctgatattac ggccgtttct
    27721 agcacaatct ttgaagcgag aaatgtttta aattgtattg ctggtgaaca aggatcattg
    27781 cttcaatatt ggggtggaga aattaaacgt gaaccattta agctttcttt gttaaagcga
    27841 agaggtcgca ataatgttgg aacgattcgg tatggaaaag atatgtctgg tttaaaggtc
    27901 aaattagatt ggacaggtat aaaaacaaga cttattccat atgctgatcc tcaaagtgag
    27961 gtaggtacga ctaatcgaat ttatggttcg ccagtagata gtgcctatat taataactat
    28021 cctgatgtgt atacagagca tgttcagttt acagaagaac aaggagtaaa agacattaag
    28081 agcttaaata aaatagctaa aaattacttt aaaacgatca atcctggctg tgataagcct
    28141 aaagtttcta ttacggttga atttgataag ttgactgata gtgaagaagc gaaagaattt
    28201 gcgaagatta gaaattatgg tctgtttgat acatttaaaa tatatcataa aaaatataat
    28261 atttatcttg aatcaaaggt tagtggacta caatacgatt ctttatctga aaaaactttg
    28321 aaattagaag caggagacat tcaagttgct ttttatcagc aacaagctgt aactattcag
    28381 gataaattaa aagattatgc aacgaataat tatatgagtg attttaatga ttatgtttct
    28441 tcaatgatta caggtcaagg cagtgcagga ggatatgtaa gtttatggcc aaaagaaaaa
    28501 ccttccaaca tctttattat ggataatcca gatttagaaa aggcaagaga agtactaaga
    28561 atgaataaaa atgggattgc tttttctaag aaaggatggc gggggccttt tacttcagct
    28621 tggacattag atagtatatt taatgccaat tttatacaaa ctggattaat caaagcagat
    28681 atctttcaaa attcttttaa caagactgga gatgtattga aattagttaa tggactactc
    28741 caaatttgga acaacaaaaa gaaaataatg gagttaacta aaaaaggaat ggaattttgg
    28801 aattctaagg gttcaattgg aacgattgga acaactgatt ctgctggtaa tccttttcct
    28861 ggggcttcta ctccaacccc tattgaagat aattctttag ttattcgtac aaatggagac
    28921 ggcaaatata ttttgatttc tcctaaagta ggaaaaggat tagttttatt aggaaatggt
    28981 aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa aggaaaagaa
    29041 ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg tgggtatcct
    29101 agtgaattaa aaacagatgc agaaaagaga gcttggagaa t
//
LOCUS       DAFBQW010000023        29110 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFBQW010000023 DAFBQW010000000
VERSION     DAFBQW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.024"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1030)
                     /locus_tag="LK889_001433"
     CDS             complement(<1..1030)
                     /locus_tag="LK889_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5095674.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="LK889_001434"
     CDS             complement(1112..1801)
                     /locus_tag="LK889_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5095675.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LK889_001435"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LK889_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK5095676.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="LK889_001436"
     CDS             complement(2328..2831)
                     /locus_tag="LK889_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK5095677.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LK889_001437"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LK889_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK5095678.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LK889_001438"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LK889_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK5095679.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="LK889_001439"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="LK889_001439"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBK5095680.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="LK889_001440"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="LK889_001440"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBK5095681.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="LK889_001441"
     CDS             complement(6443..7462)
                     /locus_tag="LK889_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK5095682.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="LK889_001442"
     CDS             complement(7635..8540)
                     /locus_tag="LK889_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5095683.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LK889_001443"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LK889_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK5095684.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LK889_001444"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LK889_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK5095685.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LK889_001445"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LK889_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5095686.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="LK889_001446"
     CDS             9753..10397
                     /locus_tag="LK889_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5095687.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="LK889_001447"
     CDS             complement(10453..10956)
                     /locus_tag="LK889_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK5095688.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="LK889_001448"
     CDS             11553..12518
                     /locus_tag="LK889_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK5095689.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="LK889_001449"
     CDS             complement(12617..13789)
                     /locus_tag="LK889_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5095690.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="LK889_001450"
     CDS             complement(13776..14921)
                     /locus_tag="LK889_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5095691.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="LK889_001451"
     CDS             complement(14935..15867)
                     /locus_tag="LK889_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095692.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="LK889_001452"
     CDS             15984..17063
                     /locus_tag="LK889_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5095693.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="LK889_001453"
     CDS             17060..17689
                     /locus_tag="LK889_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5095694.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="LK889_001454"
     CDS             complement(17799..18026)
                     /locus_tag="LK889_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5095695.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="LK889_001455"
     CDS             complement(18129..20717)
                     /locus_tag="LK889_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBK5095696.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="LK889_001456"
     CDS             complement(20737..21396)
                     /locus_tag="LK889_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5095697.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LK889_001457"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LK889_001457"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK5095698.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="LK889_001458"
     CDS             complement(22169..23035)
                     /locus_tag="LK889_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5095699.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="LK889_001459"
     CDS             23144..23785
                     /locus_tag="LK889_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK5095700.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LK889_001460"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LK889_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBK5095701.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="LK889_001461"
     CDS             complement(25226..26125)
                     /locus_tag="LK889_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5095702.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDRGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="LK889_001462"
     CDS             complement(26127..26924)
                     /locus_tag="LK889_001462"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBK5095703.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="LK889_001463"
     CDS             complement(26902..27606)
                     /locus_tag="LK889_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBK5095704.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="LK889_001464"
     CDS             27737..28312
                     /locus_tag="LK889_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBK5095705.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="LK889_001465"
     CDS             28430..29107
                     /locus_tag="LK889_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK5095706.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6170 c         5231 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccagctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttccccgatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa
//
LOCUS       DAFBQW010000024        28827 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFBQW010000024 DAFBQW010000000
VERSION     DAFBQW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.025"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(42..680)
                     /gene="plsY"
                     /locus_tag="LK889_001466"
     CDS             complement(42..680)
                     /gene="plsY"
                     /locus_tag="LK889_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK5095707.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            807..1241
                     /locus_tag="LK889_001467"
     CDS             807..1241
                     /locus_tag="LK889_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK5095708.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1407..3452
                     /gene="parE"
                     /locus_tag="LK889_001468"
     CDS             1407..3452
                     /gene="parE"
                     /locus_tag="LK889_001468"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK5095709.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3464..5914
                     /gene="parC"
                     /locus_tag="LK889_001469"
     CDS             3464..5914
                     /gene="parC"
                     /locus_tag="LK889_001469"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK5095710.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6191..8422
                     /gene="pflB"
                     /locus_tag="LK889_001470"
     CDS             6191..8422
                     /gene="pflB"
                     /locus_tag="LK889_001470"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK5095711.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8666..9427
                     /gene="pflA"
                     /locus_tag="LK889_001471"
     CDS             8666..9427
                     /gene="pflA"
                     /locus_tag="LK889_001471"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBK5095712.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9512..10438
                     /locus_tag="LK889_001472"
     CDS             9512..10438
                     /locus_tag="LK889_001472"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBK5095713.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10620..11513
                     /locus_tag="LK889_001473"
     CDS             10620..11513
                     /locus_tag="LK889_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBK5095714.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11647..11772
                     /locus_tag="LK889_001474"
     CDS             11647..11772
                     /locus_tag="LK889_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5095715.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11906..12538)
                     /locus_tag="LK889_001475"
     CDS             complement(11906..12538)
                     /locus_tag="LK889_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095716.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12686..13228)
                     /locus_tag="LK889_001476"
     CDS             complement(12686..13228)
                     /locus_tag="LK889_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBK5095717.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13363..13752
                     /locus_tag="LK889_001477"
     CDS             13363..13752
                     /locus_tag="LK889_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095718.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13756..14520
                     /locus_tag="LK889_001478"
     CDS             13756..14520
                     /locus_tag="LK889_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5095719.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPNIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14649..15596
                     /locus_tag="LK889_001479"
     CDS             14649..15596
                     /locus_tag="LK889_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5095720.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15726..16466
                     /locus_tag="LK889_001480"
     CDS             15726..16466
                     /locus_tag="LK889_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095721.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16757..17425)
                     /locus_tag="LK889_001481"
     CDS             complement(16757..17425)
                     /locus_tag="LK889_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5095722.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17559..17846
                     /locus_tag="LK889_001482"
     CDS             17559..17846
                     /locus_tag="LK889_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095723.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18262..19800
                     /locus_tag="LK889_001483"
     CDS             18262..19800
                     /locus_tag="LK889_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBK5095724.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKVYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19797..20762
                     /locus_tag="LK889_001484"
     CDS             19797..20762
                     /locus_tag="LK889_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095725.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20749..22071
                     /locus_tag="LK889_001485"
     CDS             20749..22071
                     /locus_tag="LK889_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5095726.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22134..22238
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22312..23055
                     /locus_tag="LK889_001486"
     CDS             22312..23055
                     /locus_tag="LK889_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5095727.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23070..24062
                     /locus_tag="LK889_001487"
     CDS             23070..24062
                     /locus_tag="LK889_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5095728.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24074..24811
                     /locus_tag="LK889_001488"
     CDS             24074..24811
                     /locus_tag="LK889_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095729.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24801..25523
                     /locus_tag="LK889_001489"
     CDS             24801..25523
                     /locus_tag="LK889_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK5095730.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25607..26230)
                     /locus_tag="LK889_001490"
     CDS             complement(25607..26230)
                     /locus_tag="LK889_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5095731.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            26625..27316
                     /locus_tag="LK889_001491"
                     /pseudo
     CDS             26625..27316
                     /locus_tag="LK889_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            27524..27814
                     /locus_tag="LK889_001492"
     CDS             27524..27814
                     /locus_tag="LK889_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095732.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT        10004 a         4822 c         5949 g         8052 t
ORIGIN      
        1 tttgtcacac tcccgtttta tctctttgga atgaaaaagt atcaggattt tttcttgctg
       61 ctattcaaac caaacgggat tcgactttca gtatggtttc tgatacgctt gatattgtct
      121 ttatgccgat aaaagataaa ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg
      181 ggcttagcca aaatcacagg agctacataa ggaaggacga tcgtagataa agtgatcaat
      241 actgcactga tcatactgct caaactcacc atacttgtac aaaataaact aacgataaag
      301 atgatacaag aataaacaaa aaatacagga ttaaatccta aaagcattcc tgcacttgtc
      361 gcaacagctt ttcctccttt gaacccagca aaaatcggaa atgtatggcc aagaatggcg
      421 cataccccaa accaaagagg gttaatccca gaaaggtgga agatcatcgg caaacaagta
      481 gccaatgtac cttttaaaat atccataagc aggacaacaa tcccggctgt tttgcctaat
      541 acacgaaatg tattggtcgt tccagtatta ccgctcccgt gttgtcggat atcttttttg
      601 aaaaacaatt tgccaatcca gacaccggaa ggaatagaac ctaaaagata tgctaaaata
      661 agtaataaga tcaatttcat tttttaccct ctttcaagtt gaaaactatc ctttatttta
      721 gcatgaatag gtaacaggaa caattgaaaa aatgaaagat acaagtataa tagatggaat
      781 aaaaagaaaa gcaaggggtg gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt
      841 tcaactattt ggaagaagca aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac
      901 gaacaagtta catggttgca caggtattgc aagacaatgg atataaaatc ataccagtaa
      961 atccattatt ggctggtgaa gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa
     1021 tcgatgatcc aatcgatatc gtagatgtat ttagacggag tgaattttta cctgaaatag
     1081 caggtgattt tttgaaaact gatgctaaag tgttctgggc acaattaggg attgaaaaca
     1141 aagaagcagc tgagatgcta agaaaagcag gacgtacaga tatcgtaatg gatcgttgta
     1201 ttaagattga acttggtaag ttgaaagaaa accaaaaata aaaatgacca caaagaagag
     1261 atgatcgata gaggggaatg tcgaaaaagc ggttaggaga taattaaaat tgatttctcg
     1321 tctaccctct tttttttcac gaccttttct agtatgataa tcgtggtgcg gttttgccgc
     1381 aaagtattat tgaaggagcg tttgccttgg caaaaaaagt aaacaacgaa tacaatgact
     1441 cgtccatcca agtattggaa ggattagagg cagttagaaa aagaccaggg atgtacattg
     1501 gatccactga cagccgaggc ttgcatcatt tagtctatga aatcgtcgat aatgcagttg
     1561 acgaagcatt atcaggttat ggcagtgaga ttgatgtgac catccatgaa gacaatagta
     1621 taactgttgc agatagcgga cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa
     1681 cagttgaagt gatttttact gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca
     1741 aaacttctgg tggactgcac ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc
     1801 taattgtaac gatcgttaga gatggcgtag agtatcagca gaaatttaaa aacggcggta
     1861 aacctgacgg tactttgaaa aagataggaa aaacaaaaaa agcaaacggg acgactgtcc
     1921 attttttacc agatgataca atattcagta caacgaaatt ttcatatgaa attttagccg
     1981 aacggttaag agaatctgcc tttcttctaa aaggcgttaa aatctctctt tctgatcttc
     2041 gtggagaaga accagtcaag gaaatttttc attacgaaga aggaatcaaa gaatttgttg
     2101 attatctgaa tgaagaaaaa gatacattga caccagttgt ttatttttca ggagaaaaag
     2161 aaggtatcga agtagaggtc gcttatcaat acaatgacgg ttattcagaa aatgttttat
     2221 cttttgtcaa taacgttcgg acaaaagatg ggggaaccca tgaagctggt atgaaagcgg
     2281 cgatgacgaa atcttataat gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca
     2341 agaatctgga aggtagtgat ttcagagaag ggttagctgc tgtactttct atccgtgtac
     2401 ctgaaaatct tctgcaattt gaagggcaaa caaaagaaaa acttggtacg ccagttgctc
     2461 gtaccgtagt agataacgtc atcagtgaac aaatgggctt ttatttgcag gaaaatagcg
     2521 aaatgagcca gatgttggtc cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac
     2581 gtaaagccag agaagaaagc cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt
     2641 caggaaaatt gacacccgca caatcccgaa accctaagaa aaatgaattg tatttagtcg
     2701 aaggggattc tgctggcgga tctgctaaac aaggaagaga cagaaagttc caagcaattc
     2761 tacctttacg aggaaaagtc atcaataccg aaaaagctaa aatgcaagat atcttgaaaa
     2821 atgaagaaat caatacgatg atctatacga ttggtgcagg agtcggtcct gagttttcta
     2881 tcgaagactg taattacgac aaagtaatca tcatgaccga tgcggataca gacggcgccc
     2941 atattcaagt attactgctt actttctttt atcgttatat gaaaccgctg attgaagctg
     3001 ggaaagtata tatcgcgtta ccgcctttat ataaagtatc caaaggacaa ggaaagaagc
     3061 aagtcattga atatgcgtgg acagatgacg aattggcagc aatgattaaa aaagtcggca
     3121 aaggatatat gctgcagcga tataaaggac ttggagagat gaatgcggaa caattgtggg
     3181 aaacaacgat ggatccgact tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc
     3241 aagctgaacg tcgagtaacc acactgatgg gtgacaaagt agaacctcga agaaaatgga
     3301 tcgaaaatca cgtgcaattt acgctcgaag aagatggcag tatcttagat aaaaaagagg
     3361 atactgaaat ctctccttca gtttctaatg atttattaga tgaagaacga gcagataaaa
     3421 atgaaaacaa tcagttattt gaagtagaat aggaggtcac ctgatggaaa atcgacaaga
     3481 agtacaagaa ttaactttag aagaagtgat gggtgaccgc ttcggacgtt attccaaata
     3541 tattatccaa gaacgtgcac tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg
     3601 gatattattt gccatgaaca aagatggcaa tacgtatgac aaaggattcc gtaaatcagc
     3661 aaaatctgtc ggaaatatca tggggaatta tcatcctcat ggtgaccgca gtatttatga
     3721 agcgatggtc cgcatgagcc aagactggaa gttgcgggaa gtactgatcg aaatgcacgg
     3781 gaataacggg agtatggacg gtgatccacc tgctgcgatg cgttatactg aagccagatt
     3841 atccaagtta agtggagaga tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg
     3901 gaattttgat gatacagaaa aagaaccaac agtccttcca gctcgatacc caaaccttct
     3961 cgtcaatgga tcgacaggta tatctgctgg atatgcaaca gaaatcccga ctcataactt
     4021 agctgaagtg atcgatggca caatctatat gatcgatcat ccacaagcga gccttgaaaa
     4081 attgatggaa tatattcctg gacctgactt tccaacagga gggatcttgc aaggaaaaga
     4141 tgaaatcaaa aaagcttatg aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa
     4201 gatagaggca atgaagggta ataaacagca aatcgtgatc acagaaatcc catatgaagt
     4261 aaataaagcg accttggtga aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg
     4321 aatagctgaa gtacgagatg aaagtgatag aacaggtctg caaattgtcg tcgaattgaa
     4381 aaaagacgcc aatgctcaag gaattttgaa ttatttgttc aaaaacactg agctacagat
     4441 caattacaat ttcaatatgg tcgctatcga ccatatgact ccgcatcaag ttggactgaa
     4501 agacatttta agaagttata tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt
     4561 cgatttagcc aaagctcaaa aaagacagca tatcgtagaa ggattgatga aagctttatc
     4621 gattctggat gaagtaatcg caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa
     4681 gaatttagtc gacgtctttc aatttacaga agaacaagca gaagcaattg tcacattgca
     4741 attataccgt ttgacgaaca cggatatcac agagcttcaa aaggaatccg aaagcttgat
     4801 tgcgcaaatc acagagttga ataaaatcct ttcaaatgac aaagagcttt tctctgttat
     4861 gaaaaaagaa ttgcgagaag tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga
     4921 agacgaaatc gaagaaatca aaattgatac gcaagtctta gtggcacaag aagacgtaat
     4981 tgtcagtgtg acgcgagaag gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc
     5041 caaaccagaa gaaatcggga tgcgagaagg cgactacttg ctatactcag gtgaattgag
     5101 tacattggac catgttcttc tgatcacaaa taaagcaaat gtgatctacc gccctgtcca
     5161 tgaactaccc gatttgaaat ggaaagatgc tggtgagcac atttctcaaa caatctccaa
     5221 tctgtctgta gacgaatcga ttttagctgt tttcccatat caaaaaattc aagcagagaa
     5281 aacatttgta ttcatcagca agaatggatt gatcaagcaa acacgaatga cagagttcga
     5341 accatggcgc acctataaga gccgtccgct cagctgtatg aagctaaagg cggaagaaga
     5401 tgaactgatt gctgtgtatc tgaaagaaga tcagccaggc ttagatgttt tcttagtaac
     5461 gcatcaagga atgggtttgc gctatccttt aacagatgtt cccgttgttg gagcaaaggc
     5521 agctggagtg aagtctatga atttgaaaga tggagatagg attatcaacg ggctgcttgt
     5581 gttagcagaa ggtgatacgc cgatcgtaat cgttactcag cgaggagctg ttaaacgcat
     5641 gcttgctcaa gaaatcagcc aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga
     5701 gctgaaaaaa aatccgcacc gtattatcta tatgtcggaa ggaagatcaa aagaaataac
     5761 cttgatgaat caaaaagggc agcagttaac gattgaccca actgatttcc ctataggtga
     5821 tcgaacatcg aatggatcat ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt
     5881 tatcgatgct cctgagattt tcatcgagtc gtaactgttg tagtatttta atgaagagac
     5941 gccatctgta ttaatacagg tggcgtctct ttttcattca tttttaataa atccttatac
     6001 gtttgcaaaa aaagatgaaa gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt
     6061 taaatatatg taaataaaac ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa
     6121 gatagggtgc tgtgttatac taatcacgta aaaactgtta cataaaacag aaatttagga
     6181 ggtccataca atggagcaat ggaatggatt taaaggtacc aaatggaaag aaggcgtaga
     6241 cactagagac tttattcaaa ataactacac tgaatatcgc ggtgatgaca gctttttgga
     6301 aggcccaaca gaagcaacag aaaaattatg ggatgaattg caaaagttaa atgatatcca
     6361 attcgagcgt aacggtgtct atgatatgga cacagaaatc gtatcaacta ttacttcaca
     6421 cgatgcaggc tatttggata aagatttgga aaaaatcgtc ggtctacaga cagacaaacc
     6481 tttgaaacaa gcattcatgc cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag
     6541 caatggctat acaccaaacg atgaattgac acatattttc actgactggc gcaaaacaca
     6601 taatcaaggt gtatttgacg cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat
     6661 cattacaggg cttccagacg cttacggtcg cggacgtatc atcggtgact atcgtcgtgt
     6721 ggcattgtat ggaatcgact atttgatgga acaaaagaaa aaagatcatg atatgactgg
     6781 ttatcataca atgagtgaag aagtgattcg tttacgtgaa gaaatcactg aacaatatcg
     6841 tgcattagcg caaatgaaag aaatggctgc gaaatatggc tatgatattt ctaaaccagc
     6901 tgcaaatgca aaagaagctg tccaatggct atacttcgga tatttagcag caatcaaatc
     6961 acaaaatggt gctgcgatgt caatcggacg tatctcagca ttccttgata tctatatcca
     7021 acgtgatttg gataatggag taatcacaga aaaagaagca caagaattga tcgaccactt
     7081 agtaatgaaa ttacgtatgg tcaaattcgc gcgtacacct gaatataacc aattattctc
     7141 aggattccct atctgggcta ctttatccat tgcaggtatg ggattagatg gtcgttcatt
     7201 agttacaaag aatgatttcc gtctgcttca tacattaaca aatatgggac cttcaccaga
     7261 accaaacttg actgttttgt attctgaaca ccttccagaa ggattcagaa catatgctag
     7321 cagaatcgct atcgaaagtt cttcgattca attcgaaaat gataatcagc ttcgtgaaca
     7381 atggggttct gatgattgtg cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga
     7441 catgcaattc tttggcgctc gtgctaactt ggctaaaaca ctattatatg ccatcaatgg
     7501 cggagtcgat gaagtgactg gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga
     7561 aatactagac tttgaagaag tgaaacataa cttcatgaat atgatggatt ggttagcaga
     7621 actatatgtc aatacgttga acatcattca ttatatgcat gataaatatt cttatgaagc
     7681 agctcagctg gctttgatgg attctgactt gcaacgcaca tttgctacag gtattgctgg
     7741 gatctctcat gcggcagaca gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg
     7801 taatgaaaaa ggaatagcag ttgattttga agtggatggc gacttcccta aatacggaaa
     7861 tgatgatgac cgtgcagatg aattagtttc atggatcttg gaatacttca tgggacaaat
     7921 caaacgtcat aaaacttacc gcaactcaaa accaacaact tcattattga cgattacttc
     7981 aaatgttgtt tacggtaaaa atacaggaaa tactcctgat ggccgtgaag ctggaaaacc
     8041 acttgcgcca ggagcaaatc caagttacaa tgcggaacaa agtgggttat tagctagcct
     8101 gaactcaaca gctaaaatga attacggctg tgcattggat ggaatttcaa atacccaaac
     8161 aatcaatcct tcaggtcttg gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt
     8221 cttagatgga tacttctcaa aaggagctta tcatttgaat gtcaatgtat tcagtaatga
     8281 tttattgcgt gatgcaatgg aaaatccaat gaaatatccg aacttgacga tccgggtatc
     8341 tggttatgca gttaaattcc gtgacttgac aagagaacaa caattagatg tcttgatgag
     8401 aacttcacac gacagaatgt aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa
     8461 ggacgttgaa tagccatcgc atcaaaatcg aataatcagt atgcttattt gaaagaaacg
     8521 atttacaggg gcaggacttg tatgttccgt ccctgtaaat tttacacata acgaagcggc
     8581 aattgcgaaa aattttgttc agaaagcttc gcttcgacag ctaacttgta cttaaaatga
     8641 aatcaaagaa aagatgtgaa cttaaatgga agaaaaaaca atcggctatg ttcattctat
     8701 cgaaactttc ggttcagtag acggacctgg actgcgcttt gtcgtgttta tgcaaggatg
     8761 ccgcatgcgt tgccaatttt gtcacaatcc agatacttgg aatattggcg gagggaaaga
     8821 atacacagca gatgaacttt tagataaagc agaacgcttc agaccttact ggggagataa
     8881 aggtggtatt actgttagtg gtggagaacc tctattgcag atcgattttt tgatcgaact
     8941 atttaaaaaa gcaaaagagc ggaagatgca tactacattg gatacatgcg gaaaaccttt
     9001 tacgtatgaa gaacctttct tttcacgttt tcaagaattg atgaagtata cagatttact
     9061 gttatttgac atcaaacaca tcgataatga agagcataaa aaactaacgc attgggacaa
     9121 tgaaaatatt ttagaaatgg ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg
     9181 ccatgtacta gttcctgagc gaagtgatta tgatgaatat ttgatccgac tagacaactt
     9241 tattcaaacc ttgtcaaatg tagacagagt agagattctt ccttatcata cgatgggaaa
     9301 atacaagtgg gaaacacttg gtttgaaata tccattagaa gggatcgaac cacctacaaa
     9361 agaacgtgtc gaaaatgcta aacgattgct tcatacagaa gagtatacaa aatatttaac
     9421 gcgataggtc gcatcggaaa gaagataagt attgcttctt tccgtttttt tgctattatt
     9481 aaataggaga aatcacaaag gagcgaatga catgtcaaaa attttagttt ttggtcacca
     9541 aaatccagat acagatgcaa tcggggcagc cattgcgttt tcacacttgc aaaatgaatt
     9601 aggtaaagaa acagaagcag ttgctctagg tacacctaat gaagaaacac aatacgcatt
     9661 agaccacttt cagctatctg caccacgagt agtagagtca gctaaagcag aaaatgcaga
     9721 tgtcatgctt gtggatcata atgaattcca acaaagtatt tcagatatca gcgaagtaaa
     9781 tattttagct gtagtagacc accatcgtat tgcaaacttt gaaacagcta atccattgta
     9841 ctatcgtgca gaaccagttg gatgtacaag tacgattatt ttaaaactat ataaagaaaa
     9901 ccaagtaaca attcctaaag aaatcgcagg aatcatgctc tcagctatca tttcagatac
     9961 attgttgttc aaatcaccaa catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc
    10021 agcaatcgct gaagtcgatc taaacactta tggacttgaa atgttgaaag caggaacaaa
    10081 tctaagtgat aaatcagctg cggttcttct tgatttggat gcaaaaagtt tcccaatggg
    10141 cagttccaac gttcgtatcg ctcaagttaa tacagtggac cttaatgaag tgatggatcg
    10201 acaagcagag ctagaagcag caatggaaga tgaaatgaaa gcaaatggct acgatctgtt
    10261 cgtattagtt gtcacaaaca tcttagacag tgattcagaa ttacttgttg ctggtaaagg
    10321 aaaagaaaaa gtagaagaag ctttccaaac aactttgacc aacaaccgtg ctttgcttaa
    10381 aggtgtcgtt tcacgtaaaa aacaagtcgt acctcaattg acggctgtat ttagctaatc
    10441 aaatggtagt atgaagcatt caaaatgaat ggataagcga ttgatataaa aagaaacata
    10501 gaacatacta aaggccgaag aaatggagga gaataacagt caattatgac tcttgttctt
    10561 cctccttttt acgtaaaagc cttttaagta aaataaaacg gatagaaaag agaacatata
    10621 tgaactatta ttcaaaaaat aaggaaaact tttatcaaaa actgaccgat gatccgcttt
    10681 tttcttcatt gaccgattat ctatatgaac atcgagaaca agaaacgatt ctaagagaat
    10741 tgaaaaaaga attttcgcaa aataaatttt cccattttct tgatttattg atcgatgcag
    10801 gattaatcaa gagggaagaa cgaagatatc atttgaactt tccaatattt gatcctaaag
    10861 attacttgca acaagccact tcggctgctg aaaccatagc tgaacaattg aagagactct
    10921 ctgttgatga acaaaaatta gcgatgggag aagttatttg ggcgtattgt ttcgaagatg
    10981 aacggaaaga agcgtatttt tatggagtaa gaaacagccg agagacagaa ttgctgagga
    11041 caactgcagg aaatgagaag taccgtttta tcacactttc ctctatagaa cacttcccgc
    11101 ttactttggc taattacttt tttgtacaga agaatcagct tcctgtaacg aaggcattca
    11161 aagaattagc cgaacttatt ggagacgtaa atgaatcgta cttttttgat caaatagaag
    11221 ttatcgttga caggatacgt aagaataaat ataaaaatcg ccggcctagt attttccatc
    11281 agtctctact tgtgacgaat actataaagg aagaagaatc attcacatta gaattaccta
    11341 ttgtcgaaaa aaacaatttt gagattgaat taccaacatt ggatccttca ttgacaatgg
    11401 aggaaatggc ctttcttaaa cgacaaatct tttctgagct gagcaagaaa tttataccgc
    11461 atgcattttc ttatatcaaa gaatatggaa cagttttagt gagtaaaacc taagagagca
    11521 gtaagcaaaa attatactga ctcttgcagg aggagccttt ctcttccttt atggttaaaa
    11581 gaattatttg agtattttgc ctatctacca cctattagat tcactacatg gtctggaaac
    11641 aaaggaatgc aaaaaaatgg tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa
    11701 gttagatttt taaataatca atattatgat tcagtcaagt atggcatttt atgggaagaa
    11761 ctagctgatt agattaggaa gtccatttga taatccagaa tcactaagaa acgaatggcg
    11821 agtcattaat aatttatcca atcttgggtt attcttttag attgaagtgt ttaaaactaa
    11881 aaaagcattt atgaagtcgt aatagtcagg cttcaaaatg ctttttattt tgtttgtttt
    11941 ttctttgcca cataaataca cctagactga cgatgaagag catatccaac agatttcctg
    12001 cgacttggat cgtcgaaggg ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca
    12061 agctgtaaat aaaacaggtg acaggaacta atcgaaagaa gagcggacat ttcaattggc
    12121 taaaaatgag tgaaaaaata aaataagtaa aaatggataa aatcacgact aaaccattca
    12181 tgataaagaa gaaaagcgta ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa
    12241 acgttttaaa tgcaatcaat acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa
    12301 aaaaataggc accgaaataa acagaaaaca gtaagccgca aagagaagac aaaatcaaaa
    12361 aaaacggatt ttcttcggct tctgtgatat cagaatgatc caaaagcaac cagcctaatg
    12421 cactagaagg cgcatgtgag gtcttaaggt atgaataaca tagaaaaaat aagatcgtca
    12481 aagttgcaaa aaaagagcct aaatgtaaaa aattagtggt tctacgttga atttccataa
    12541 tatctccaaa ttaaattgat aagtatataa agtataataa caaattgatg agtatatgtc
    12601 gatgtttttt aatcaaaata ataaataaaa actgtctaac atttaaaatt taatttagat
    12661 attagacagt ttttaagttt gtgagttatt ttccttgtcg ttttctcact tttttagtcc
    12721 agaaaccacc tgaaatatat tttggctcat aagaaatgat aaaagcacgc tcttcgattt
    12781 ctttgatcag gcgatacagt tcacgttcgc tttttctagg agaaagtatt tcgagaacta
    12841 gccgctcacc ttccagcccc attgcagaag tctgtgtcac accataaccg tgttctctca
    12901 gtatacccgg caaactttta tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga
    12961 tgtatcctaa tgccaatttg tcttcaatca aaattccgac actgatacct actgcgtaac
    13021 ctaacgcata gacaaataaa ttcagtggat tatctaaacg atctaaaaca agggataagc
    13081 cgacgatata aatcgtgatc tcgaccatac tgacaagtgg agcaatcagt cgatagcctt
    13141 tcattgtcaa catgaaacgt aacgtgttca gagtgatata agcaaaatta atgaggaaga
    13201 tcatccccaa catttttgga tcaatcattt gtaaaactcc tttcatttat ataacaacaa
    13261 tatcaaaaaa aatcgtaagt gaagaaaaat tgaaacttta tacatacaaa aaaatgataa
    13321 aatgattata atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt
    13381 gtgttttgtg ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg
    13441 attcaacagc caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta
    13501 attttaagcg tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc
    13561 agttttttga atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt
    13621 tgttctctgt gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg
    13681 aacctgttcc agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa
    13741 ataggtgagt aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg
    13801 ttagaagttg ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac
    13861 ggatggcaga caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc
    13921 attcgtgtga tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct
    13981 attccatcct atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt
    14041 atcaagcatt tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg
    14101 atggggggaa tgacaacatg cgccttgttg gctaaacatc ctaatatagt agcgggcgtg
    14161 tgtttgatgg gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg
    14221 gaatataatt atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat
    14281 gatttgtcat tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa
    14341 gctgacgaaa aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag
    14401 tcttacggaa aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata
    14461 ccactaatgg aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa
    14521 ttgaaacgga aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa
    14581 taaactctgt taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg
    14641 aagagagcat gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg
    14701 tctctcgagt catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct
    14761 cagcaatggc tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc
    14821 ggtcgcagat catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca
    14881 tgaatttgct gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag
    14941 ttacaaatga tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa
    15001 aaaaagactt cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt
    15061 ctgaaggata tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat
    15121 atgccttaga aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt
    15181 ttgaaatttc aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta
    15241 aaatcttgcg gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg
    15301 cagattttac agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag
    15361 taagagggat tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg
    15421 atggggtatt tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta
    15481 tacgtatggg tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga
    15541 gtcaaggtta tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct
    15601 tattactata tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg
    15661 ctactgaaaa caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg
    15721 tttgaatgaa agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag
    15781 gcgtaaaatt aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga
    15841 ctgaacagaa tcagtccccg tttgaacaga tgaaagaatt tcatttcacg aacacgaaca
    15901 ccgagaaaat ccgtcgcaag cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg
    15961 aaaatttgga aaagcataaa ggaaaattac cggattatac cggccattac caatcagaaa
    16021 tcacatcgac aggacgaaaa aaactttttg gtgattcctc tcgttctgtc agacaaagtg
    16081 aaacaaggac agagtccaag catactacac gaaacgcatc tgttgacacg aaggtgcggg
    16141 agcgttctta ttttgttcct aaatatgtac ccgcatcgat tatcccagac cctagagaaa
    16201 gcaaaatttc tgaggatcat ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt
    16261 tgttcgatac agaaccgacg ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa
    16321 cattcaatcg aacaaaaacg ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa
    16381 aaagccatgg tatcttagaa cgctcattac aaggattgat cgatgatcaa ggaaactctt
    16441 tgggagaaaa tagctatttt aaataagtga tatacaaatt tatattcggc tacagttgga
    16501 aaggaaacgc tgcggcgttt ccttatttta tactctatcc gaaagtttat atcgtatcct
    16561 caaatttttt tgagtttaag cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa
    16621 taaaaaaaca aactattttc gcagatcgtt tgcaaaaata gtttattttt ttataaagaa
    16681 gattttactg aataatatca ttttagttta cataatgata tttctgtaaa ttaaataaag
    16741 tagtgaaaaa taagaatcag tgctctaact gttcaagagg atcaagtggt tcaaatgttt
    16801 ctgtagtctg ttttgcttcc aattggggca aaacagatcc aatttccaga tacttcactt
    16861 tattcaatgt ataagataga atagaacagc taagtaatgt atacagaaaa tgtgtgagat
    16921 ccaaataaag caaggagaca actaaaatac caaaagtaat caagaaaaga cttttgccaa
    16981 tcttaatatg aaaatatttt tcaatggctt gataaatgat atctgttccg cctgtgcttc
    17041 cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag aatacctgtc atcaaactat
    17101 tgatcagtag actatctaca gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga
    17161 aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca
    17221 atgagataag caataaaggt aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa
    17281 aaagattatg caaaagcaaa gcaataccag taaccccacc atctgccagc ttagatggaa
    17341 tattgattcc tgtcacagct aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt
    17401 ctgtaaataa atttaatttt ttcatttcta gcctcttttc ttttgaaaat tttcagcaac
    17461 ttctttatta tactgactta tactcttact tcaagaggaa aatgaaaaaa aattattttt
    17521 ggctgtttct ttttatttat caaataagga gtggaaaaat gcgagcagca gacttattga
    17581 caattcttac aaaatcaccc aaaaaaacag agtatcaagg agtttttttg gctcaagaac
    17641 aaaaactggt gcctgtgaca atggtccaga taaataaagc aaatcaattt atctttttat
    17701 ttgaacacgg caaagcacca cttgctatga aagacatctt tctttttttg atgaagaacc
    17761 gtgagaaagc tgtttattat cagtcaaaag aagaactcat tcctctttat ggagtgaaag
    17821 agatagaaaa taaagtggtg atataagtaa aaataagaaa acaagacgtt tatgatgtca
    17881 aaacaaccat tttttacatt tttggcaaaa aagtgtcatg atacagtata atattcgttg
    17941 aacaggccaa taaaggtgtt ctcccccacg agatttctac tttattggct gtttcttttt
    18001 attaaaataa aagttgtgtg tgtaaaaaag atatagctga ttccaagtga caatttcgta
    18061 tagattttta tggtattcgt agtgaaaaaa cgagaaccaa aaagttgatc cgactttgtt
    18121 gcttggattc tttttttgtc agcatattta gaggtatttt tttctttttt ttgttcgtgc
    18181 tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa agaaatgttt atacttaaag
    18241 aataacagaa aggaaggtcg catgaataat tttatacaaa cattaagtga aagaaaaggc
    18301 gagttgctgg aagctgcatt tcaacatctg tccatttctc tcatttcttt gctgattgca
    18361 gcactgattg ctattccttt agcgatttgg gcagccaatc ataaaaaaat ggcagaagtc
    18421 ttattgcaga tcacgggtgt gctccaaacg attccttcac ttgccttatt aggtttactt
    18481 atcccatttg tagggatcgg aactgttcca gcattgattg cacttgttat ttacgctttg
    18541 cttccgattt tccaaaacac gtatattggt ctagctgaaa ttgacccgtc tattgaagaa
    18601 gcagcggtag catttggtat gtccagaatg agacgcttgc tgaaagtaga gcttcccatc
    18661 gcattacctg tcattatctc aggtatccga actgccttag ttttaatcat cggtacggct
    18721 actttggcag cattgatcgg agcaggtgga ttagggacat ttattttgtt aggtattgac
    18781 cgaaatacac ctgttttgac attgattggt gcgatcagtt ctgccttgct tgcaattata
    18841 ttcagcggac tgattcgctg gttgcagcat aagaaacctc gatattccat cattacttta
    18901 gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc agtctgcctt gtttaaaaat
    18961 gaaaccatca cgattgctgg aaaattagga gcggaacctg aaatcctgat ccaaatgtat
    19021 aaagaattga ttgaagacaa cacaaaaaca gaagttgaat taaaaccgaa ttttggcaag
    19081 acgagttttt tgttcaatgc actggatcaa ggtcaaatcg atatctatcc tgagttcaca
    19141 ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa tgaaaagtgc tgatttgaca
    19201 aaagaaaaaa catatgacga agcagaagaa ttattgaata agcaattcga tatgacatta
    19261 ttaaaaccaa tggcttacca aaatacgtat gccttggcag tcaagagatc ttttgcgaac
    19321 gaacatcaac ttaaaaagat ttccgatttg gcagctatct ctgatcagat caaagcggga
    19381 ttcacactgg aatttattga tcgttcagat ggatataaag gaatccaaga agcatatggc
    19441 ttgaattttt caagtgtaca aagcatggag ccaagtttgc gttaccaagc aatcaataat
    19501 ggagatgtca atattgtgga tgcctattcc acagatagcc agctcaaggt gtacgatcta
    19561 gtgactctag aagatgatcg agggttattc ccagcttatc aaggcgcacc gctgatgaaa
    19621 aaagattttg ctaaagaaca tcctgaaatc gttaaagcac tgaataaatt aagcggaaaa
    19681 ataacagagg atcaaatgat tgatatgaat taccaagtca atgtcgaaaa gaaaaagccg
    19741 acagaagtag cacgaatctt cctgaaaaat gaaggattag taaaggagga agatcagtga
    19801 ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga tggaaagtat gtcatcgatg
    19861 acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt ggttggtcct tccggtagtg
    19921 gaaaaacaac tactttgaaa atgatcaatg gattatctaa accatcagca ggagacatct
    19981 acttcaaagg aaaatcgtta aatgaatata atctgcagaa aatgcggtgg aatatgggat
    20041 atgtgctgca gcaaatcgct ttgttcccga ccatgacagt caagcaaaat attgaagtca
    20101 tccctgaaat gttaggttgg gaaaagcaga aaagagcaga ccgtgtagat gaacttcttc
    20161 aaaaagttgg cttatctcct gatatttatc gagatcgtat gcctagagag ttgtctggag
    20221 gagaacagca acggatcggg atcatccgag ctattgctgc aagcccagat gtgattttga
    20281 tggacgagcc atttagcgca ttagatccga tttcaagaaa ctcattacaa gaattggttt
    20341 tgtccttaca cgaagaattg ggcacgacca tcgttttcgt tacgcacaat atggaagaag
    20401 caatcaagct aggtgatcgt attgcattta tgaaggatgg agaaattatc caatgtgaca
    20461 caccagagca gttattgatg aatcctaaaa acgactacgt tcggcatttc tttgatgaac
    20521 cgaaacagac gaaagagtgg cgagtagaag atttagtcgt caatggatat ttcttaaatg
    20581 aaataccaga aaatgctcgt caacaagttt gctttgatac gcctatgaaa gaagtatatt
    20641 cgcttctttc ggtgtatcca agcattgtga tagtagaaaa gcaaagaagt attgggtcgc
    20701 tgacaagtaa agaaatcttt gcttttttaa gtcgagagga ggaaggaaat gaaaacatat
    20761 gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg ctgcttatgg attgaaagaa
    20821 gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg gcgggacttg tcctaaccgt
    20881 ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg cacgtaatcg ggtaaaacaa
    20941 ttgagtggaa aaggttttaa cgagatccca actgccaact gggaagaact tcaaaaattc
    21001 aaacgaacat ttacggatcc agtcccagaa agccgaaaaa aacaattagc agaagctgaa
    21061 atcgatcatc tttcaggaac cgctcgtttt ttggatgatt catctattga agtcaacgaa
    21121 gaagtcttcc atgcggatta cttagttttg gcaaccgggc aaagacctac gatcttacca
    21181 gtggagggaa aggaatactt gaaaactagt gcagactttt tatcattgcc tgtactgcct
    21241 aaagaaatca tttttatcgg tggtggttat attgcttttg agctagctac gattgccaat
    21301 gctgcaggga gcaaagtaac cattgtccat cataatcaac ggccattaaa agaatttgag
    21361 gcgtctttgg tagaggaagc cgtccatcaa atggaagcat ctggcattca gtttgctttt
    21421 ggtgttgaaa cccaaaagat catttcagaa ggtactcgct atcgtttagt cggaaaagag
    21481 acggaattag tagcagatat gattttttgt gcgactggga gacagccgaa tacagaatct
    21541 ttagcacttg agcaagcgaa tatcgtcttt gataaacacg gaattgcagt caatgattat
    21601 ctgcaaacga gcaatccaaa aatttttgcg tgtggagata ttgtttcaag gaaaacccca
    21661 aaattgacac cagtcgccac ttttgaagga aattatgtag ctaagcggat aacggatgca
    21721 acaagcgaac ctatcaaata tccgattata cctacgatcg tctacgccag tcctaaactt
    21781 gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag atcaagtggt agagatggat
    21841 cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag ttgctaaggc tgaattgacc
    21901 tttgaccagc aaaattattt gattggtgct gcagtgatta gcgagcaagc agacgaattg
    21961 atcgatgatt tgacactcgt catcaatcaa aaattgacta aaaaagagtt ggatagctat
    22021 atcatggggt atccgacatt ggcaagtgat ctctcctatt tattgaaata atcagcatga
    22081 taactaaagc gcttgttgct ttttccttct tgtgctacac tttaaaaaat gaatacctgt
    22141 ttctggggat gtcgtagacg acatgagagt aagttgtgaa cttagaccca ttgaacctgt
    22201 cagttaatac tggtgaaggg agaaacatag cagacgcttg aagcgggtta ctatgtcgtg
    22261 cccatttcaa gcgttttttg ttattcatat tagaagaaag gaggtaacaa aatgaagaaa
    22321 aatctttatc cagcagtcag tatggcattc attttaatta tttggcagat tgttgtcata
    22381 gtttggaaaa tacctgcctt tattttaccc ggacctgctg ctgtattttc tgcattagcg
    22441 gcagatgcac aagtattact ttcccattct gtcacgacat taaatgaagc agtgcttggt
    22501 ttggtaatag ctagcgttct ttcttttatt actgccttgg caatggatta ttggaagttt
    22561 ttagaaatgt ctttttatcc gttactagtg atttcacaaa tgttgccaat catggtgtta
    22621 gggccattac ttaccctatg gtttggtttc ggtatgcttc caaaagtgat ccttgttgtc
    22681 ttgataagct attttccgat cgtagtttct ttttcggata cattgaggaa agtatcaaaa
    22741 gagcaaatca tctttttgaa aacgatgggg gcagatacag ggaagattta tcgaatatat
    22801 aaaataccag cgggtttatc cggcttcttt tccggtttaa aagtggctgc tacttattgt
    22861 gtcagcggag cagtcgttgg agaatggcta agcgctcaag caggcttagg gtattacatg
    22921 atacgcgtaa aaaacagcta ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt
    22981 ctattgagtt tattgctgaa tggcgcttgt tcattattga aaaaagggta ttatcgtatg
    23041 ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt atggctttta ttaccgctac
    23101 tgttgctttt atccgcttgt ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg
    23161 atttgaaaaa agtgacactt gttttggatt atgtaccgaa tactaatcat actggcatct
    23221 acctagcaaa agaaaaaggc tattataaag aggctggttt aaatgtccaa atcatcgaac
    23281 caggagataa ttcgacaagt attggtctag taggtgcgga caaagcacaa tttggtgtaa
    23341 gttatcaaga agatgtcact tatgctcacg cagatggaca gaatatacca gtcaaagcaa
    23401 ttgctacagt gatcaaacat aatacttctg gattcgccac cctatcagac agtaacatcc
    23461 attcaccaaa agattttgaa ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag
    23521 ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt ttcaaaactg acaatggtag
    23581 gaagcaatgg agaagggcca gaaagcttag gaaaatcctc agatatccaa tggtactttg
    23641 aaggttggga tatgatcaaa gcaaaagaag cgggtattga actaaactat atcccattga
    23701 aagaattaga tgaacggtta gattactata ctccggtaat tattacgaat gatcaactga
    23761 tcaaaagtga tccagaattg gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg
    23821 aagctattaa ggatccaaat gacagtgcta aactgttgca aaaatatgca aaagaaaatg
    23881 atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa aaattatacg gatgatcctg
    23941 agaattgggg attgatggaa gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa
    24001 acggattgat caagcaagat gtaccttcgc aagaactatt cactaatcag tttataaaat
    24061 aggagtgata gatatgtcaa tccaaatcag ccacttgaat gtagtcattg aaaacctgaa
    24121 aatattagaa gacatccatt tcactatccc taaagatacc tttacatcga ttgtcgcgcc
    24181 aagcggagcc ggtaagtcaa cattgctgaa aactttaaca ggagtccagc ctatgacttc
    24241 tggaacgatc aaagtagacg atcaaaaagt aactggtctc catacagcat ttagttatat
    24301 gccgcaagaa gatatgttac ttccttggct gagcgtctat caaaacgtta cgttatatca
    24361 aaaaatcaat catctaacaa tcaatgaaga acaagtaagg gattatctgg atatctttgg
    24421 gttaaacggc tatgaacatt ttttgccgga acaattatct ggcgggatga agcagcgaac
    24481 ggccttgtta cgaacaatca tgaatccttc ttcttacttg cttcttgatg aaccatttgg
    24541 agcgttagat gctatgacca gagggcagat gcaagactgg atgttgaagc tgcctaaaaa
    24601 agcaaagcgg actactttac ttgtgacgca tgatatcgaa gaagcaattt atttatcaga
    24661 caggattctt gtgctttccg cccgtccggc acatgtgatt gctgagattc aagtgcctga
    24721 aaaacgacgg tcacgtgaat ggctactgca gcaatcagag ttgaagcaaa cgatttatca
    24781 gttattggca ggtgaattag atgttaagtg atgctcattg tcatttagat gggagtcaga
    24841 gtttagcact ccttcagcag gaacacacga ttttgacgat catcaattgt gattcgccag
    24901 aagaatggaa ggaaaacaga cagcttgcag caagtaagac tcaagcctta agttacggta
    24961 tccatccttg gaaagcagat agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag
    25021 caaggatcat cggtgaaata gggttagata atatttggac gaatgtaccg atgactacac
    25081 aaaaaaaagt atttgagcga cagcttgctt ttgcagccat aaatgagaaa cctgttgtac
    25141 tgcacacaaa aggatgcgaa aaagagatct taaattatat ccaagcttac cctaatcact
    25201 atttgatcca ttggtattct tctttggact atcaacaaga ctatattgat ttaggctgtt
    25261 atttcagcat tggtcttgat ttgaaaaaga atccagcagt ctggcaacta gctaaagcag
    25321 tcccaatcga ccacttactt attgaaacgg atggactaca tgcagcagag tgggctttag
    25381 gggggatttt ttcagaaaaa gattatgtct cactgcttga agaacagtta aagacaatcg
    25441 ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac
    25501 gtttcattag attaggtaaa taaaaaagag atgtgacaca actagtgtcg cacctctttt
    25561 tggctttctt tgtataaaag taaactgttt gattaataaa tgaatcttag taccaaccgt
    25621 ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta acatattgtt
    25681 ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa gaatctaatt
    25741 ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg
    25801 cgatccattc ttttgcagag cttgtgcttg ctggagtagc agcagtttct gttacagcag
    25861 gagcaggttg tgtttcagca acaggcgctg cttgtttttc cgtttgaacc gtttcttgta
    25921 caacaggttg ttcagcttgg actggtgctt cttgaacggc tgcttgaggt tcagcagctg
    25981 cttgtgcaga gccttctgtt gggattgtta attgttgtcc tgagtaaatc aggttaatat
    26041 cagaaattga atttgattct gcaatggcat tgatcaagga gttatcacca acgtattttt
    26101 gagaaatcgt tgatagagta tcacctgatt gaacggtata agcttcgtcc gcatgagcag
    26161 tcgttcccat gaagaatgca gcgccggcag ccaaagttgt tccaaaaagt aaagttttaa
    26221 gtgatgtcat gaaaaatctc ctttattcgt taaaaagttt tatttgttgt gttcaacgag
    26281 gaatacttta acacgtgaaa atatttcata ggtaaaagaa gcatgacaat ttatgacaaa
    26341 tccgcgtttt ttgtaataaa tacagtaatc tatgaaatga aaagggttac gtttaataaa
    26401 acttgctttt ttgtttttgt tcaccataaa tatttattta agaaagtatt aacctgcatt
    26461 tttctctata aaaaagattt tttaaataga taaaaaagga ggattaaatg agtatttgat
    26521 agtatttata ttaaaattac atatgtaatt taaaaacgct tgacattgtg tttgagatca
    26581 cttactttta gcatgtaaaa gtattggatt aaggagctat gcaaatgaaa aagaaattta
    26641 ttactggtgg gattgtcgtt gcatttctat gtctaattgg ctacacaggc acgcggtacg
    26701 caaatgaaat tattttgtgg ggtggagaga cagatattga tacaatcaat gagaatctga
    26761 cacagcttga tcatacattg acagataaag agcaagcgat tacagatctt accgctcgac
    26821 tagcacaaaa agaacagaca ttgcaagatt caaaggcaga tgtccaagaa taccaaacaa
    26881 aagtaaatga attagaaaac caaaaaaatc aatcggcagc agaaaaacaa aacttagaaa
    26941 gtcaattagt cagcaaggat gcacaactac aaagcaaaca aaatgaaatc aatgtcaaac
    27001 tagatgagat caaccaaaaa aatgatgaaa tcaatcatct gaatgcagat tggacagcaa
    27061 gactacaaga agcacaagca aaactttcag aattacaaaa tactgtaaat ggtttgaatc
    27121 aacaaatcgc aacactgaca caagaaaaaa caaatgtaga aatcagttaa cagatacaca
    27181 aaagaaatta gacgaagcaa ctgctgaaaa ctcaagcttg aaacaatata ttcaaaagtt
    27241 agaaaaagca aaagatgaag tggaagatac agctgataaa tctcagcaaa ttgttgaaga
    27301 acatttgcgt aaataatctt tcttaacaag taaacctaaa ttagtaaaga agcaacagta
    27361 atctatactg ctgcctcttt tttttagtac atgtccttca tcttcgcagg aagtgccaat
    27421 atgattttaa gaaaggtaat agacaaaaag tcaaaaagat aaaacagagt ttcttcttta
    27481 caaagggaat gtatgttcgt ataatggtat tgaggtgaag gttatggaga aaaaatatgg
    27541 aacagtagat aagatccgta ctttgcaagt tagtgccaga ccgttgatcc gtttcagttt
    27601 aaatggggtc aattgtttga ttgcttccca tagtttgaac ttcctagctg aagtagacga
    27661 agggatgaag ttagtcgtta caggttatta taataataga aagcaatttg ttgtgagatc
    27721 ctatcatgtg ttagggaaac ccaaaattgt ggtagaatat gagaaaagtc tatacccaag
    27781 aaaaaaggtc cgacaagatg aaagaataaa ctagaaaaca agcggctgag acaaaaatgt
    27841 cctagccgct tgttttggaa agatggatgc agacgatggg atttgaaccc acacgagcat
    27901 acgctcacaa gaacctcaat ctatatataa ctacatgtat ttaagtattt gtgatgaatc
    27961 actaaacttt ccaaactcat ttgagggata ttgcttaaag attgaacaga tcaattcgaa
    28021 gccctattag tattcgctca atggggggat cagaggccat aaaaagagac tagtcattac
    28081 tagtctcagg tagtggaatt gtaggatttt aaacgttcct ggagagtacc ggtatgtaac
    28141 tcgaagaggt gattatcata gtcgtaaaag taaattgaag aagcctcgcc tttcacacga
    28201 gaacgtcctg gttcgacagt taaacctaac atttggattt ttgagacaaa cgaatctata
    28261 tcttgttcgt caattttaaa agcaatatga ttgtatgtct ttggcagttt attcgacgaa
    28321 ttctgcatta ctgcaatcca taggtcctta actaagaaaa atttttctgg atataatgaa
    28381 aatttttttg ttgactatcg tagatttctt ttgcatcaaa taaggtattt aacaattaag
    28441 tatttaaaag ataactttta agttatcttt aagtaatgct taaagctatg ttatactcta
    28501 ttaggaaata taagaaggag tataaaaatg aacaaacata atagaaaaat aacgaataac
    28561 aaagaactga tcttgttact aatagggatt tggggtatct ttttactctt taatatgtat
    28621 atgccgacta tgagagcgga tgacgtagtt tatgcaagta gattagatga acttggctat
    28681 ttgggtgctt cgatagaaca ttataagaca tggagttcaa gaattattat agaactattc
    28741 ttaatgttct tttctaaaca ctttatgcta tggaaattat taaattctac gattatgtta
    28801 ggaattgtag tattactttg caagtat
//
LOCUS       DAFBQW010000025        28683 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFBQW010000025 DAFBQW010000000
VERSION     DAFBQW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.026"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(22..1188)
                     /locus_tag="LK889_001493"
     CDS             complement(22..1188)
                     /locus_tag="LK889_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5095733.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(1200..1553)
                     /locus_tag="LK889_001494"
     CDS             complement(1200..1553)
                     /locus_tag="LK889_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK5095734.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1681..2703
                     /locus_tag="LK889_001495"
     CDS             1681..2703
                     /locus_tag="LK889_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBK5095735.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            2722..2883
                     /locus_tag="LK889_001496"
     CDS             2722..2883
                     /locus_tag="LK889_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095736.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(3142..3576)
                     /locus_tag="LK889_001497"
     CDS             complement(3142..3576)
                     /locus_tag="LK889_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095737.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(3686..5545)
                     /locus_tag="LK889_001498"
     CDS             complement(3686..5545)
                     /locus_tag="LK889_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5095738.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(5663..6822)
                     /locus_tag="LK889_001499"
                     /pseudo
     CDS             complement(5663..6822)
                     /locus_tag="LK889_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(7129..8553)
                     /gene="araA"
                     /locus_tag="LK889_001500"
     CDS             complement(7129..8553)
                     /gene="araA"
                     /locus_tag="LK889_001500"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK5095739.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(8569..9267)
                     /locus_tag="LK889_001501"
     CDS             complement(8569..9267)
                     /locus_tag="LK889_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK5095740.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(9224..10885)
                     /locus_tag="LK889_001502"
     CDS             complement(9224..10885)
                     /locus_tag="LK889_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBK5095741.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            11103..12503
                     /locus_tag="LK889_001503"
     CDS             11103..12503
                     /locus_tag="LK889_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBK5095742.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(12755..13834)
                     /locus_tag="LK889_001504"
     CDS             complement(12755..13834)
                     /locus_tag="LK889_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5095743.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(13920..14879)
                     /locus_tag="LK889_001505"
     CDS             complement(13920..14879)
                     /locus_tag="LK889_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK5095744.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(14891..16402)
                     /locus_tag="LK889_001506"
     CDS             complement(14891..16402)
                     /locus_tag="LK889_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK5095745.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(16424..16987)
                     /locus_tag="LK889_001507"
     CDS             complement(16424..16987)
                     /locus_tag="LK889_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK5095746.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(16993..17856)
                     /locus_tag="LK889_001508"
     CDS             complement(16993..17856)
                     /locus_tag="LK889_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5095747.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(17870..18766)
                     /locus_tag="LK889_001509"
     CDS             complement(17870..18766)
                     /locus_tag="LK889_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5095748.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(18941..20230)
                     /locus_tag="LK889_001510"
     CDS             complement(18941..20230)
                     /locus_tag="LK889_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5095749.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(20202..21719)
                     /locus_tag="LK889_001511"
     CDS             complement(20202..21719)
                     /locus_tag="LK889_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK5095750.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            21917..22807
                     /locus_tag="LK889_001512"
     CDS             21917..22807
                     /locus_tag="LK889_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBK5095751.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(22880..23704)
                     /gene="nadE"
                     /locus_tag="LK889_001513"
     CDS             complement(22880..23704)
                     /gene="nadE"
                     /locus_tag="LK889_001513"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK5095752.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(23716..25188)
                     /locus_tag="LK889_001514"
     CDS             complement(23716..25188)
                     /locus_tag="LK889_001514"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK5095753.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(25825..26187)
                     /locus_tag="LK889_001515"
     CDS             complement(25825..26187)
                     /locus_tag="LK889_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK5095754.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            26410..27162
                     /locus_tag="LK889_001516"
     CDS             26410..27162
                     /locus_tag="LK889_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5095755.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(27302..28225)
                     /locus_tag="LK889_001517"
     CDS             complement(27302..28225)
                     /locus_tag="LK889_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5095756.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(28240..28512)
                     /locus_tag="LK889_001518"
     CDS             complement(28240..28512)
                     /locus_tag="LK889_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095757.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         8478 a         5987 c         4883 g         9335 t
ORIGIN      
        1 tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt cttcataaag
       61 agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag ctgctgcata
      121 aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag tcagcagaac
      181 taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg gagagatcaa
      241 gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga tcatgaatgg
      301 acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc ctaagtaagg
      361 gatcaggttt aaaaaacccg caataatccc caacgtgaca gcatactcta aaccaatgac
      421 cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt gtcctcttat
      481 ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt tttgcatgcg
      541 tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca atagataaaa
      601 taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc cgacaaaact
      661 tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg ccgaattcat
      721 gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg ggtcccgcaa
      781 aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac tgatgaagct
      841 cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga ttagtgcaac
      901 aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct tttcaaagta
      961 atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag gcagacctac
     1021 gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa ataaaaagac
     1081 gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat ttaaaaacca
     1141 gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa ttcctcctgt
     1201 tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg atgaagatag
     1261 attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga tacgtgagct
     1321 actgtcttta aattcccatt ggccatcgga ccgacataat cgatcgtata tacctgatca
     1381 tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga tacttcttga
     1441 atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat cagcatgggt
     1501 tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat cattgcactc
     1561 tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc cgtgtggaat
     1621 aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga atcagctcat
     1681 atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg aatctatacg
     1741 atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt tgaagaaaat
     1801 agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac tagtgtggat
     1861 ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc ggtaacagag
     1921 tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact atacggggcg
     1981 aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga actgaaagca
     2041 gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga tcatgcgcat
     2101 gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct tggtacagat
     2161 cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac taccttcact
     2221 gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca attcgcttat
     2281 ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga aacaggtgct
     2341 tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga aaatggaggg
     2401 gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg aggacataac
     2461 tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa gcaaatcatc
     2521 tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa ttttgtcgtg
     2581 tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga aacaggatta
     2641 ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta ttttgatcgt
     2701 tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact acgggaagaa
     2761 ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga agaggaatat
     2821 cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa tgtagaaaac
     2881 taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc gtctatcctg
     2941 atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga aaaggagtat
     3001 ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca ttatcataaa
     3061 ggattttgat cactctatca taaaggtaat attacattta tattagataa ctctagtttt
     3121 tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt tttggatggc
     3181 agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga atagatcttc
     3241 atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg ctacatatga
     3301 aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg gacgcaaagt
     3361 actgactttc ataagcattg cccgatattt ttttgttcct tcttcaaata acaggatgat
     3421 ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg cgtttctgat
     3481 cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga cgatttgatc
     3541 gatggactgc acataatagc caatattcgg taacatgttc gttttcctac tttctccatt
     3601 gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg aaaaaattta
     3661 atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct gctaaactat
     3721 attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa atctcttgtt
     3781 gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac agttctcgtt
     3841 caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca tttgttgtct
     3901 gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag
     3961 tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt tcatgcaatg
     4021 tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga tgatcccccc
     4081 aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg
     4141 ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc ccacttccat
     4201 ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc atatgtgtct
     4261 gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt gtcattactt
     4321 ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc gtataggtca
     4381 tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc tctcttttat
     4441 acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg tttaacgctg
     4501 aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt
     4561 gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata tttgctgttc
     4621 caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct acttcataat
     4681 aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactagga tctgagaaac
     4741 tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta tttttttcat
     4801 cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa tatccttcag
     4861 gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa ccagtgttat
     4921 agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat gcttttcgca
     4981 acaaattatc gggattgttg aaattactga tataccctag cagtccaatt gccacaagca
     5041 gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga aacatgtacc
     5101 agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac agcatagtgc
     5161 cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct ggataaatcg
     5221 gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg atggctacga
     5281 catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa ataagaataa
     5341 tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca
     5401 acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta ctaaaaacaa
     5461 caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc tgtttggtcg
     5521 aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa taaaagaatg
     5581 ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat
     5641 gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag cggacctttc
     5701 gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca aaatcagtac
     5761 tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat atatctgcga
     5821 tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg gttttctttt
     5881 gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat ggataattga
     5941 ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact gccaaacgtc
     6001 cgctgtctgc caaagcttcc tgagtcgatc ccctagatga ggcaacataa gtatacggtc
     6061 attttctaag aactcttctt ctggaaaatc agtcaagtat ttagccagat gttgtttttc
     6121 caatgcttgc ataacagcat atttatcaac aatttcactt cttcctacat tgataagtac
     6181 agcatctttt ttcatttttt ctatattcgc ctgatcgatc aatccttttg tatcttctgt
     6241 caacggcagc atgactacga tgtaatccga ggtagaaaga actgtttcta aatctgcttg
     6301 ccgaacataa tccaactggg catcgcggat agaataaccc aagacatcca tccctaagct
     6361 atagcaactt agcgctactt ttgtgcctat cgctccaaga ccaagaagac cgatggtttt
     6421 gccttgcaat tctcgtccca cataagcact tcttttgttt tctgcctgtt ctaaaatatt
     6481 aggacctgtt agtgtttgta ccatacgact tgcttcgatt atcggacggg aagataagag
     6541 caaacagcac aaaacaagct cttttactgc attagcattt acacctggtg tattcatgac
     6601 gatcgtcccg ttttcagaag ctttttcaac gttgatcgtg ttcaccccaa caccagctct
     6661 tgaaattgct agcaaatctg gtgtaagcca ttcatctttc actttcgtac tacgaataac
     6721 tattccttcg gctggcaaat catctattat tttgaaatat tccccttgta acttttctat
     6781 ctcttgttct ggaatagcat caatcaaatg aacgtgatac ataaaacccc ctccttacta
     6841 acaagtatct cgaaatttct gcaattagac aactgtttac catttgactt cctagaagca
     6901 aaaagagcag ttagagcaga gtaggctgag tgataaaaat aaggctatga caagttactg
     6961 ccataagcct tattttcgag agcaaaatat tgagggtgag atatcttgat aaatggtgtt
     7021 ccgttagctg acattgataa taagactaaa tttcaaaaaa gttgaaaagc aaatttcgtc
     7081 aatctcttct tatttcttaa aactaagtgg ctttttcaca acctcttttt atctaataac
     7141 cactgtttcc aactcaacta attttgccca cgtttcaatt tgatctgttg ttagatttag
     7201 cgagacaacg gtatgatgac cgccacctgc ctgaatccat gaatggacac cttcatggaa
     7261 attcggttta gttttccata acacacgtgc aacaggtaaa ttaggtgctt cagtcgttgg
     7321 ttcaaaggct tctacttcat tgatcagcag tttataatgc gtaccgaaat ctgccatcga
     7381 tacgactacc ccttctcctg ctttcccatc aaatacaaga cgcgccggat cttctctatc
     7441 tcccattgat aaaggagaaa caacgattct tggtttcgtc gcagcaagcg tcggatctac
     7501 ttccatcata tgggattcca agattgcttc ttggccacta gctagctcgt aagtataatc
     7561 ttccatgaat cctgtatctt tccctcgagc catgattttc agcaaccgat caatcgctgc
     7621 agttttccag tcgccttccc cagcaaaacc gtatccttct gccatcaaac gttgtacagc
     7681 tagtccaggt agttgtttca ttccatacag gtcttcaaaa ttagtcgtaa aagcgttata
     7741 gcctccttct tctaagaaac ggcgaatacc gatttcctgc tgagcttgta cttttacatg
     7801 ttcctcccat gtcttttctt cataatcacc atagtcgaag gtatacaatt ccttgtactc
     7861 ttcaaataat tgatcgactt cttcgtctga tacgcgatcg attacttgta caagatctcc
     7921 gataccaaaa taatcaacag tccaaccaaa ttgaatctgt gcttccactt tgtctccttc
     7981 tgtgacaccc acgtttctca tgttgtcacc aaaacgagcc acttttattc caaaactttc
     8041 attataggct actgcaacat ccatccaatc agcaatctct ttttggatct caggtcgttt
     8101 ccagtagcca acgacgattt tattttgttt atttaatcga gcattgatga acccatattc
     8161 gcggtcacca tgagcggatt gatttaaatt catgaaatcc atatcgatcg ttttccaagg
     8221 aatgctttcg ttatattgcg tagctaaatg aagcaatggt ttttgtagaa gctttgtccc
     8281 gcggatccac atttttgctg gtgagaaagt atgcatccaa gtgatcacac ctgctacttc
     8341 ttctcgataa ttgacctctt tcatgatttt tgtgatcgta tcagcagtaa ctgctaattc
     8401 ttgaaggcgg attggataag gcaattggcc attttcattc agttcatcga ccatttcttg
     8461 tgcatgtttc tttacttctc gcagtgcttc ttccccatat aaatgctgtg atcctacaac
     8521 aaaccaaaat tctttttctc caatattcaa cataattagc cactcctttt atttgttttg
     8581 tccgtaatac gcattttttc cgtgttttcg taaataatgt ttatctaaaa tccgttgagg
     8641 cagctcttct gaaaatgaat tgagctgtct ggtaaataaa ttcatcttgc atacttcatc
     8701 tagtacgact gcgttcatta ccgcactttg cgcatctttc ccccaagtaa atggaccgtg
     8761 tccgtgtaaa agtacccctg gaattgctaa gggatcaatt ttgcgttctt tgaatgtttc
     8821 tatgataact tttcctgttt catattcgta cccgctgtcg atttcctgtt gtgtcaaaaa
     8881 tcgagcacaa ggtaccgatc cataaaaagt atctgcatgc gtcgtcccca tcgcaggaac
     8941 atccaatcct gcttgtgccc agatcgtcgc ccatgtcgag tgagtatgga cgattcctcc
     9001 gatttgagga aatgaacgat ataaaacagc atgagtaggc gtatccgaag agggattgag
     9061 tttaccttca atgatatgat tgtccaagtc cacaacgacc atgtcgtctg ctgtcatctc
     9121 ttcataactg actccgctag gtttgataac gaaatatcgt gtttcaggat caaaagcgct
     9181 cacatttccc catgtatatt tgacgagtcc gtgtttaggc aagtcaagat ttgcttggta
     9241 tacttcttct ttcagttgtt ccagcatttt ctatcactcc tttttcttgc aattgatgaa
     9301 tcgctgtttt ttcaatgtcg atcccctcac ggtaacgtct cataaatatc tgatagcctg
     9361 ctatttcttc tgctgaaggt gtgatggtga ttccttggtc ttgtccaaac acgtcttctg
     9421 ccaagaagtt ttctaaagac ttcccatgtc gatccatcat ataggctgct aataacgcga
     9481 ttccccacgc tccaccttct ccggctgtgt ccatcactgt aactggtgcc tccatagcag
     9541 atgctagaat ccgctgtcca acttctggtg ttttaaaaat ccccccatgc cccactaaac
     9601 gatcaatttg gacattctca gatttcaata tatccattcc gattctcatt gcaccaaaag
     9661 cactggctaa atggatccgc atgaaattcg ctaaatcaaa acggctacct ggtgtacgta
     9721 caaataatgg ccttccttca tttactcctg taatgttttc accggaaaaa taaccataag
     9781 aaagtaatcc cccgcaatcc gtatcgcctt ctagcgcttt gttgaataat accgtgaata
     9841 atttatctct gcttatctcc atacccaaag aattggtaaa ttcttcaaaa atactgaccc
     9901 aagcattgat atccgaagag cagttattcg tatgaaccat gcctactagt tctcctgaag
     9961 gtgtagtgac catgtcgatt tccggatgga cattttctag ttctttttct aatacgatca
    10021 tagcgaaggc ggaagtacca gccgaaatgt tccctgtccc tttggctaca ctgttcgtag
    10081 cgaccatacc ggttcctgca tccccttctg gcggacaaac cgggattcct ggtctcaaat
    10141 ttcccgtagg gtcgatcaga taagcccctg attcgcttaa cttgccagct tcttctcctg
    10201 ctactagaac tttaggcaaa atgtgatcca agtcaatcgc tacgtgcgct ttttcgatta
    10261 actgaccaaa aatatttttc atttcttgat ggtaggtttt cgttgaagga tcgataggaa
    10321 acatgcctga tgcatctcct acgcctagta ctttctcacc agtaagctgc caatggacat
    10381 aacctgcaag cgttgtcaaa aaattaattt ctttcacatg ttcttcttta ttcaaaatag
    10441 attgatacaa atgagcgata ctccatctct gcggaatatt gtattgaaat ttttccgtta
    10501 atattcgttc tgcttcttca gtgattgtat ttcgccaagt acgaaatggt actagtaact
    10561 gttccttgct atcaaatgct agataaccgt gcatcattgc actgaaccca atcgacccga
    10621 ttgtcgtaag attctcgcca tagatttcga atacttctgc tgccattttt cggtaactga
    10681 cgcgcagtcc tttccaaata tcttctaatg aataggtcca gataccattt tctagtttgt
    10741 tttcccactc ataactacca gcggcaatag gagtaaaaga tgaatcaatc aatacagctt
    10801 tgatcctagt tgatccaaac tcaatcccta aagatgtacg accatctatg acatctaaca
    10861 tactttgcgc atttacttcc ttcataatga tttaactccc tccttctcgt caaacctgat
    10921 caaaccgtct ttttagtagc gcttacaatg attattgtat acttttgtac gtacatgtca
    10981 agcagttatt tacaagaata caataaaaaa cacttgtttt gtacgtacat aagttataaa
    11041 atgaaattgc ttacaaaatc tgatcaaccg ccttctcgaa attctagaag gagagaatac
    11101 ttatgaaaaa agaaaaaaaa gtttcctcat cgtttattta tttttttggc gcatttggcg
    11161 gcattctatt tggatatgat attggtgtta tgaccggcgc cttacctttt ttacagcatg
    11221 attggggttt ggcaggtaaa gcttctttga taggatggat cacgtcttcg gtcatgttag
    11281 gtgcgatcct tggcggctct ttatctggtc ttctttctga taagcttgga cgacgaaaaa
    11341 tgattcttct ttctgcactt atttttatgg ctggttctgt cctttctgca tctgcccctc
    11401 acaatggttc ttattttctg attgcagcaa ggattcttct aggtttagct gttggcgctg
    11461 catctgccct agttcctgct tatatgtctg aaatggctcc tgctagacta aggggaagat
    11521 tgtcagggat caatcaagtg atgattgctt ctggcatgct cctttcctat gttgcagatt
    11581 atttgctaaa agggttgcct gaaacgatgg cttggcgcgt gatgttaggg ctcgctgctg
    11641 tccccgcgct gattttattt ttcggtgttt tagctctacc agagtcaccg cgttttctta
    11701 tgcaatctgg ccgcttggaa gaggcaaaaa gagtattgaa ctatattcgg acaccaaaag
    11761 aagctgaaca ggaattcgaa caaatccagc tgaatgtcaa acaagaaaag acaaccggaa
    11821 cttcttggca tactttgttt ttagaaaaat atcgttcctt agtcattgcc ggtattggtg
    11881 tagcagtatt ccagcaattt caaggagcta atgctatttt ttactatatt ccgctgatcg
    11941 tagaaaaagc gactgggcat gcagcgagcg atgcattgat gtggccgatc atccaaggaa
    12001 tcattttagt tgctggttct ttgctctttt tggttattgc agacaagttc aatcggcgaa
    12061 ctttattgaa aataggcggt agcgtgatgg gactttcttt catcctacca gctgttttag
    12121 gaactgtact ggatgctcat acaaattcgt tgttgattct cttgttttta tgtatttatg
    12181 tcgcatttta ttcttgcaca tgggctccac tgacatgggt aatcgtaggc gaaatattcc
    12241 cactagctgt ccgcgggcga gcatctggcc tagcctcttc ttttaattgg attggttctt
    12301 tcctagttgg cttgcttttt cctgtgatga ctgcttctat gtctcaagaa atcgttttcg
    12361 gcatttttgg gatcatctgc tttttagggg ttttatttat tcaagagatc gtaccagaaa
    12421 ctcgtgggaa atctttagag gaaatcgaac aaagtgcatc taaaaaaact tatcctaaac
    12481 gtatttctat ccatcattct taaagaggtt gtgacagaag tggtcagctt caagaaataa
    12541 gaaggaaccg cttagtttcc agtcacaagg aacaatttca aaaaacagct tctcatgttt
    12601 gatgaatatt gtgacttgtg accagttggt aggagaacac cgtttagtgg agaggtggtg
    12661 aaagaaacgt tcagccttga gcgatgacaa aagctctcac aaacattgcg acgagtaacc
    12721 gtaggagcaa taagaggcgg taaaaacccg tctcttattt ttcgttttct ttcactgttt
    12781 cccgttcaac tagtacaggt tcatagagaa tatttggctg tgcctgtcct ttttcaatcg
    12841 ttgtgacgat ccaccgtgcg gcatcttgtc ccaacttttc ttttggatgc gtcaatgttg
    12901 tcaatttttt attccctaaa tggcttaaag aagaatcgtc tgttcctacg atcgataggt
    12961 catctggaat atgatagcca ttttctgtca acttatctag cattaaacta gctatttgat
    13021 cgttatagca aatgatgcca tctaaagctt gttcttcttt caaccgttgt actgtttttt
    13081 cagcgattgt ttctcgtgtt tccgtggtga aagtaatgat atcttccgtc aaaaaacgca
    13141 cattatactg ttcacacgcg ctgataaaac ctttcatcct gtattttcct tgaagatcgt
    13201 cgatttttgt gatgaacaac agacgctgat gtcctttttt tatcaaatat tctgctgcca
    13261 taaaaccgac tttcacatca tctaaacaaa tataagaggc atttaactct tcataagcag
    13321 cattgatcat caccatcgga atgccttgtt ctctcaaagc aacataaagt gctaagtttg
    13381 ggttatactg attacttttc gtaggctcaa cgatcaaacc atctacccca taagcaatca
    13441 ttttcttcaa acactctttc tccaattcgt gatcgttatt cgtactggct aaaagcaatg
    13501 aatacccaga ttctcgtaat tcattttcga ttcctcggat aatcgacgga aaaatataat
    13561 cagagagata tgtggtgatg acaccgatcg tttttgttct gttcaatggt gttttttctt
    13621 tcttatagct gtcatctaca tatgtgcccg accctttttc tttacgcaga tagccctcat
    13681 ttaccaacac agcgattgct tgccgaatcg tatgtctgct gacgtcatat atttcttgca
    13741 gcttgaattc tgagggaatg gttgtattta cttcgtattc ctcacgaagg atcttttgtt
    13801 ttatctcttc tgcaatatct tgatattttg ccatttgcct tgcccctttt tatttgtacg
    13861 tacatatatc ataacgagtc tgcaaggttt attcaagcaa aaatcaaggt ttcttgtgct
    13921 cataaggcat acggaacagg ttcaccaaat tctggaaatc cttgctcatt ccacgtaaag
    13981 cattgcgcgt tcgcgtgccg attaggattg tctaacggat cccctttttg gttcttctct
    14041 ggtcgagcat gatagatcaa aatatcaaag cacccgtctt ctgaaaccgt aaacgagttg
    14101 tgaccaggac caaattgctt atttttttca gaagaaacga atactggctc cgataattta
    14161 tgccatgatt cttttaataa aagattcttg tgctcatcag cccacagcag gcccatggca
    14221 tagttttcat ctgttgcact accagaatac gtaatgaata ttttcccatt gcgagtaagg
    14281 actgctggac cttcatttac tgaaaaaccc cgtttttccc attcatactc aggtatagaa
    14341 aggaggatct gcttcccctt taacgtccaa ggattatcca tttcggatag ataaagatta
    14401 gaattcccag taatttcagg atcttgttgg gcccacacat aatatagctt tccttgagaa
    14461 gagaaagttg ttccgtctaa actgaaagtt tcccttttcg tttttatttg tcccttttct
    14521 atccaatctg tattcattgg atctatatgc tgattttcaa gtacgaacat gcggtgatga
    14581 tgagattcat cacgtatagt tttatgattg cttgcagcaa agtaaatata ccattttcca
    14641 ttgatataat ggacttcagg tgcccagatc aattcactca tcggtccttc ttcatgagcc
    14701 tgccatacaa tcaggcttgc agcatgttct aattcattca gtgactttgc ccttcttaat
    14761 tcaatcactt ggtagcctgg aaccgatcct gtaaaataat aataaccatc tgtatgttta
    14821 tatacccaag gatctgcccg ttgccgaacg ataggattat tataaagtgt cttcatcata
    14881 acaaatttcc tcacattttc tttattctaa ttacattcca agagtatttt tctatcccta
    14941 catttaattc gtcatgtttt tctattgaaa aatagtttat ttcaggtttt attttttctg
    15001 gttcttcacg tgtatttttg tccaacagat cgctactggt taatgtgtag tgctctcctt
    15061 gatctagcaa attgaaatca tgttcaatcg ttatcttttg ttgttcattt gtgcgattga
    15121 tcaagaaaat agtcagctga ttgccgttat ctacgatcac actgtccaca taaggaacat
    15181 tttttatctt ttcacaatca taaacagggc aaatgatttg ttgtttcaat acttgacctt
    15241 tagcatattt cgataagtgt tgcatcacat aataagttgg attcctccat gcttgtcctt
    15301 tagcatctgt cagtattaaa ggtattgtgt ttactaacaa tgattgacaa ctaatcttta
    15361 cgcgatctgc gtgtcgtaaa atagccagcc ctaatgaacc aaataataat gtatcttcca
    15421 tagtaaagta acaccaatcg atgggactac cgacttcaaa atccctatgt cttttttccg
    15481 gttgggcatg tacattccat tcatcaaacg ctagatacat tgttttctca cttctcaaat
    15541 atccttttac ataatcacaa gtagcaacga tttcttcaat ttgacgatcg aatcgataag
    15601 cttttgcaag gtatgtcgct gtatcatcac gctcattttt aggaggttta gtcaagtctt
    15661 cttcatcata cttatcaata tagttatgca aagctaaata atctacattc tcataagctt
    15721 cttctaatgc gatacgatcc cattcagggt acgatggtaa acgtggcgta gagcttccaa
    15781 ctaagattgt ttcaattgaa tcatccacta acttcatgac ttttgaggct tcatttgcta
    15841 acctaccata ttcataagca ctcttagccc ccacttgcca tggaccgtct agctcattcc
    15901 ctaaacacca taatttaacg ccaaaaggct ttttttgccc attttttatt cttaaatcac
    15961 tccagaaggt tccttttgga aaattacaat attctactaa attttttgct tcatcaattc
    16021 cttttgttcc taaattcacc gtgaatatcg gtttcgcttg tacatgactg atccatttaa
    16081 aaaattcgtg gagtccaaaa gaatttggtt ctatacctcg ccatgctaaa tcaatttttt
    16141 ttggtcgttg ttctttccta cctacagtat ccacccaatc ataaccagat gtaaagtttc
    16201 ctccaggata gcgaatgaca cctaattcta attcctttac ataagagagg acatcttgtc
    16261 taaacccgtc accatcagct gtttcatggc ccggttcata aatccctgta tagacgacac
    16321 ttcccatatg ctcaacaaat gagccaaaca gccgagaact gatctctcct accgtaaaat
    16381 cctctctgat ttttactttc acttcttcgt ccaacagctt tccctacctt tcatttccta
    16441 tagtcaagat aatagataca agtttccata cgccaccagc aaagataaaa ataaaaggag
    16501 ggaaaagaaa tatgagcagt acaaatccta ataacaatac gcaacaaaga atatttctca
    16561 aaaaatcaaa aaatactttc catgcacttt gttcaaaaat ttgtaaaaat gtgatatctt
    16621 tgttttcaga aatcacatat atccaggata aaagaaacat ggaataaata agtaaaacta
    16681 catatccact aattgaaagt acgattgtct gttcttctaa taattgatta acaagaaaat
    16741 aaataatgat cgttaacaga ctaaggaaca agttcttacc aaaatgttgc tttattcctt
    16801 gaaagaaaaa ataaatgata tttcctgatc cctcaccttt ccattgcgcc attacataaa
    16861 aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt acccagagaa
    16921 tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta gtctcaacta
    16981 ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa aatatttctg
    17041 tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag ccattacagg
    17101 tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta aagttctcat
    17161 ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat tcatcagcgt
    17221 gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt tcaaaaaaat
    17281 attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg gtaaagtaat
    17341 aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa tgatcaaacc
    17401 tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa tcatgaagac
    17461 ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt ttcctttgaa
    17521 acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag tgatcactgt
    17581 ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg ttgtccaaac
    17641 cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa atgagataat
    17701 gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg caataaccaa
    17761 caatataact agcatactaa tcgttcgaat gattttttta ccactaactg cttttcgact
    17821 attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc tatttcattt
    17881 gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca acaatgataa
    17941 acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta aatcctcgac
    18001 tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg gttgcaacat
    18061 atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa aaacctaagg
    18121 agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt gcaccatcca
    18181 tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag agaatcgtca
    18241 tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata gcaagggttg
    18301 gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa ggaatcgccc
    18361 ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat gaacaaataa
    18421 ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta gataatgcta
    18481 ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg aaatataaag
    18541 aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa ttttcaaaac
    18601 ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata gcaaatgttc
    18661 ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt aaaaacaaat
    18721 acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag tccacctcat
    18781 ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag atatccgaac
    18841 aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc attcagatat
    18901 ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc agtaattgat
    18961 tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct aataattttt
    19021 caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa cctggtaaat
    19081 atggctctgc atagtccatt tgtttcgata attcagatac aataggatca ttttttactt
    19141 cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata ctccattttt
    19201 taccaatttc agttgtattc caatatttca aaaactcata gacagcttct tttttattat
    19261 tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt tttttgtctg
    19321 aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct ttcaatccgt
    19381 tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt aaattgtcca
    19441 tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt ttttggatcg
    19501 cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa tcatcgttca
    19561 ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca tccttattga
    19621 agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt tctgtcaatt
    19681 cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac aaatcattat
    19741 tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta ccttctactt
    19801 cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca tattcccaga
    19861 aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct ggataattta
    19921 atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt tcattcaaat
    19981 tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt ttattaaatt
    20041 cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc caaaaagtca
    20101 aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa gctcctaaaa
    20161 tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc attttttgtt
    20221 cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt caataaacaa
    20281 ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac ttgttcactt
    20341 ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc aatcactccc
    20401 tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc acggttaact
    20461 gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata cggcacttct
    20521 tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac ctctccttgt
    20581 ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc ttctccgttt
    20641 gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc aatctttact
    20701 ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc atcttcaaaa
    20761 ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg ctcatcttgt
    20821 tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg cttcttacta
    20881 tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa ccgatccatg
    20941 tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata atattgatgc
    21001 agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc ccaatcacca
    21061 aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga atcatctacg
    21121 agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc tgttttatgt
    21181 ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat tccaaaaggg
    21241 ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg aaaattacaa
    21301 tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac agccatattg
    21361 atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac ttcattcgtt
    21421 tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg cccaatactg
    21481 tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat gggaatgttc
    21541 aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc tgatggatga
    21601 tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa tgaactgtac
    21661 aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt ggcttccata
    21721 actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt gtcaataata
    21781 taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt aataatgatt
    21841 aaaacaaata atttaatata acttttatta acaaatatgt taatatataa gaaaaacctt
    21901 ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg tatatgaagc
    21961 attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa agatgaatgt
    22021 caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc acgtgaaaaa
    22081 gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa tttcgagcct
    22141 tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt ttgatacgaa
    22201 agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa catgcggttt
    22261 agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta tggatcctcg
    22321 aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc aggcacctaa
    22381 ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa tttcttctga
    22441 atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa atggtgtgga
    22501 gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat atacgcctga
    22561 ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa ttaccaaaca
    22621 cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca tccctaaaga
    22681 gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg tggggggctg
    22741 tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa aagtttacta
    22801 cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct atgaagggac
    22861 ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt ggcaaatgtc
    22921 gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga gcgggaactt
    22981 cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg acttcatctg
    23041 caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat aatttttccg
    23101 gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg aataatggca
    23161 aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt tctgcagcat
    23221 gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc gtaatcatac
    23281 gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct gcttcgctta
    23341 atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta acatcgggct
    23401 ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca taaggaagac
    23461 gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct tccatcgtta
    23521 gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct agtacaaaag
    23581 ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt ttacgtattt
    23641 cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct gtctgcaggc
    23701 tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt tcattcgttc
    23761 catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt atttttgcgg
    23821 attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg tatattcttt
    23881 gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga tatcttgtaa
    23941 aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca ctggatggaa
    24001 catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt agtctccttc
    24061 agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg gagtagttac
    24121 tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat catcttcgat
    24181 cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga tcaattttgt
    24241 cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa tcgttgcttc
    24301 atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat ctaattgttc
    24361 ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc cttggaagtt
    24421 gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg atttcaacgt
    24481 atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg ccataaatgc
    24541 ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc tagctggtat
    24601 cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga tatatgccgc
    24661 tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa attccaatag
    24721 tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca acgtttggaa
    24781 attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag gcccttccac
    24841 ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac gaactgttgc
    24901 tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt ctaatccacg
    24961 caagtaagca atatcggaat cattgaacgt cagattctct aaataattga ctaaccgttc
    25021 caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt aacattcaaa
    25081 aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat tgatttgata
    25141 tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct tgtattactc
    25201 ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag gaaaagaaat
    25261 cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa aatgaactcc
    25321 atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga atacttattt
    25381 tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta cttgataaaa
    25441 taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga acaaatataa
    25501 ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat acgtctttcc
    25561 ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga atttttttgg
    25621 aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca
    25681 cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa caaaaatggc
    25741 gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg agcaggttac
    25801 cataaagatg attattagtc gttattaaat agaatccttc gtattttttt gttctatcac
    25861 tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct cctgtttcac
    25921 tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc
    25981 gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct ttttatcata
    26041 tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat aattaatagc
    26101 cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat ttaaattact
    26161 attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt cgatttagat
    26221 ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac aattgaacct
    26281 ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt cgtgagaaaa
    26341 aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca tttgtcagga
    26401 ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt cagtccgaga
    26461 accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa ttcttcaggt
    26521 tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt ttatcgaaca
    26581 atggcattca agttggatga tatccaagag gtcttagaca atccatcttt tgaccaccag
    26641 aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat cgataccctc
    26701 ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat gagtgacaaa
    26761 gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa
    26821 gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat
    26881 atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt atttgatctc
    26941 actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt ccaagctcat
    27001 aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa aggactagga
    27061 atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag tggtcatggt
    27121 gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac atgatccaac
    27181 aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt tagattttta
    27241 tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac tttaaatata
    27301 ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct gtaagaaaca
    27361 attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg aaaccaagaa
    27421 gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc
    27481 tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca actccaagct
    27541 ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc caaaaaacaa
    27601 gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt tttgagttat
    27661 aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct tgctgcttca
    27721 aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag cttgcctgtt
    27781 ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc aataaatcat
    27841 ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct gcaaattctt
    27901 caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc actttgtaat
    27961 cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac ggatgattgg
    28021 tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt ctggcatttg
    28081 cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt ttttcctcat
    28141 ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa cctgtgagct
    28201 ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa gattttcttg
    28261 taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc cttcatgcga
    28321 aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga tttcattcat
    28381 tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga
    28441 tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg ttatctctac
    28501 ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg aaacgcgtct
    28561 catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt attaccaaag
    28621 tctcttattt taaattgaat acattataat gatactgtaa atatatcttt attattagtg
    28681 aat
//
LOCUS       DAFBQW010000026        28599 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFBQW010000026 DAFBQW010000000
VERSION     DAFBQW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.027"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            234..1874
                     /locus_tag="LK889_001519"
     CDS             234..1874
                     /locus_tag="LK889_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBK5095758.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2028..3350
                     /locus_tag="LK889_001520"
     CDS             2028..3350
                     /locus_tag="LK889_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK5095759.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3412..3843)
                     /locus_tag="LK889_001521"
     CDS             complement(3412..3843)
                     /locus_tag="LK889_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5095760.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3907..4743)
                     /locus_tag="LK889_001522"
     CDS             complement(3907..4743)
                     /locus_tag="LK889_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBK5095761.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4933..5235)
                     /locus_tag="LK889_001523"
     CDS             complement(4933..5235)
                     /locus_tag="LK889_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBK5095762.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5261..5692)
                     /gene="ruvX"
                     /locus_tag="LK889_001524"
     CDS             complement(5261..5692)
                     /gene="ruvX"
                     /locus_tag="LK889_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK5095763.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5689..5955)
                     /locus_tag="LK889_001525"
     CDS             complement(5689..5955)
                     /locus_tag="LK889_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBK5095764.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6209..6934)
                     /locus_tag="LK889_001526"
     CDS             complement(6209..6934)
                     /locus_tag="LK889_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK5095765.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7300..7370)
                     /locus_tag="LK889_001527"
     tRNA            complement(7300..7370)
                     /locus_tag="LK889_001527"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7336..7338),aa:Gly,seq:ccc)
     gene            7487..8239
                     /locus_tag="LK889_001528"
     CDS             7487..8239
                     /locus_tag="LK889_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK5095766.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8255..8734
                     /locus_tag="LK889_001529"
     CDS             8255..8734
                     /locus_tag="LK889_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK5095767.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8985..10565)
                     /locus_tag="LK889_001530"
     CDS             complement(8985..10565)
                     /locus_tag="LK889_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBK5095768.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10558..11301)
                     /locus_tag="LK889_001531"
     CDS             complement(10558..11301)
                     /locus_tag="LK889_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095769.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11477..12253)
                     /locus_tag="LK889_001532"
     CDS             complement(11477..12253)
                     /locus_tag="LK889_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5095770.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12501..13337)
                     /locus_tag="LK889_001533"
     CDS             complement(12501..13337)
                     /locus_tag="LK889_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBK5095771.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13348..14277)
                     /locus_tag="LK889_001534"
     CDS             complement(13348..14277)
                     /locus_tag="LK889_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK5095772.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="LK889_001535"
     CDS             14470..14823
                     /locus_tag="LK889_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK5095773.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="LK889_001536"
     CDS             complement(14897..15553)
                     /locus_tag="LK889_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK5095774.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15779..17713
                     /locus_tag="LK889_001537"
     CDS             15779..17713
                     /locus_tag="LK889_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5095775.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17799..19022)
                     /locus_tag="LK889_001538"
     CDS             complement(17799..19022)
                     /locus_tag="LK889_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5095776.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19016..20914)
                     /locus_tag="LK889_001539"
     CDS             complement(19016..20914)
                     /locus_tag="LK889_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBK5095777.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20908..22026)
                     /locus_tag="LK889_001540"
     CDS             complement(20908..22026)
                     /locus_tag="LK889_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5095778.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22109..22942)
                     /locus_tag="LK889_001541"
     CDS             complement(22109..22942)
                     /locus_tag="LK889_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5095779.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22935..23852)
                     /locus_tag="LK889_001542"
     CDS             complement(22935..23852)
                     /locus_tag="LK889_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5095780.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24079..25203)
                     /locus_tag="LK889_001543"
     CDS             complement(24079..25203)
                     /locus_tag="LK889_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5095781.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25386..26294
                     /locus_tag="LK889_001544"
     CDS             25386..26294
                     /locus_tag="LK889_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5095782.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26435..28048
                     /locus_tag="LK889_001545"
     CDS             26435..28048
                     /locus_tag="LK889_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5095783.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5620 c         4868 g         9219 t
ORIGIN      
        1 tatttatcta tctaacatat cctttattct tctacttttc tttctttagc atataagaga
       61 tacgcatagt aatcaagcaa ggattgttga tataaaaaga acaaataccg ctattttgat
      121 ttcttttgac tctattactc atataaataa aatttattat acctgctctt tttgactata
      181 tcctcatctg actcctatac ttcgtttgta ttaaaactta ggaggaatca ttcatgctta
      241 cagggctaaa cttattaaat aatccttttc taaataaagg aacagctttt acaaaagaag
      301 aacgggcaaa atacggaatc actggtatgc ttccaagtac tgtccaaaca ttggaacaac
      361 aatctgtcca agcttacggt caatacttaa gcaaacagac cgatttggaa aaacgtatct
      421 tcttaatgaa tctattcaat acgaaccgga cattgtttta taaattgatg ggcgaacatt
      481 tagttgaatt catgcctgtc gtttatgatc ctgtcgttgc ggattcaatc gaacaataca
      541 acgaaatttt cttgaaacca caagatgctg tatttctttc aatcgacgaa ccagaaaata
      601 tcaaagcaag tttgaaaaac gcagcagatg gacgcgacat tcgtttgatc gttgtgacag
      661 atgcagaagg aatcttaggt atgggtgact ggggtgtgaa cggtgttgac atcgctatcg
      721 gtaaattgat ggtctacact gccgcggctg gtatcaatcc tgcacaagtc ttgcctgtat
      781 caatcgacgc tggtactaat aacgaagaat tattgaataa tccattatac ttagggaatc
      841 gtcatgctcg tgtagaaggc gaaacttact acgaattcat tgatcaattc gttcaatcag
      901 ctactgaatt attcccagaa ttgttattac attgggaaga ttttggacga ggaaatgctg
      961 caacgatctt agaaaaatac gaagataaaa tcactacttt caatgatgat atccaaggca
     1021 caggaatcgt tgtactagct ggtgtattag gtggattgaa tatatcaggt gagcaattga
     1081 aagatcaaac gatcttgaca tttggtgctg gtactgctgg tgtaggtatc gcaaacatcc
     1141 ttttagatga aatgatccgt caaggtgtac ctgaagaaga agcacgccaa catttctatc
     1201 aagtagataa acagggttta ttgtttgaag atacaaaagg attgactcct ggtcaaatcc
     1261 cattcgctcg taaacgctca gaattcacta atgctgatga attgacaaac ttagaagcag
     1321 ttgtaaaagc aatccatcca acgatcatga tcggtacttc tacccaacct ggagcattca
     1381 ctgaagaaat cgtgaaagaa atggcggctc ataccccacg tccaatcatc atgcctttgt
     1441 ctaatccaac aaaattggca gaagcaaaag caaaagattt gattgaatgg acggacggaa
     1501 aagctttagt tggaacaggt atccctgctg ctgatgtgga atataacggt gtaacttacc
     1561 aaatcggtca agcaaataat gcgttgatgt acccaggttt agggcttggt ttgattgctt
     1621 caacagctac acgagtaaat gctgaaatca tttctcaagc tagtcgagca ttaggcggaa
     1681 tcgttgatgt aacaaaacca ggagcagcta tcttgcctcc agtagcaaaa atcactgaat
     1741 tctctcaaac gattgctgaa actgtcgcaa aatcagttgt tgcacaaaaa ttgaatcgtg
     1801 aagaaatcac ggatatcaaa gaagctgttg aatctgctaa atgggttcct gaatataaat
     1861 ctttagaaga ttaagattgt tatagtaaaa gaacatacag aaactttgta tgacgtctaa
     1921 aaagaaaaaa gcgactgcgg caaaacagca gccagccaac tactgctctt ttgccgcttc
     1981 tctttggctt ttttcatacg atacagaata agaaaggatc tgtaaaaatg gcacaaaaaa
     2041 caacaaccgc aactgcagtt gaggaaaaga aaaaagaagg gttttggtca acaaaaatta
     2101 ccggcgtaag tttgcccctt tatttgatca tggtccttgt cctgatcatt gcaatggctt
     2161 taggaaaatt accgacgaat atgattggcc caatcgctgt tttggtcatt ttcggtaatt
     2221 tatttcattt tatcggaaca aaaatcccta tagtcaaaag ttatttaggt ggtggctccg
     2281 tttttgcgat ctttgcctct gccgcgattg ctacatttgg gatcttacct gagtatgtag
     2341 taaaatccac agaaaacttt gtcagcaata tgggcttttt agacttttat attgctgcct
     2401 tgattactgg tagcatcctt ggtatgaacc gtaatctatt gatgaaagct tctgttcgtt
     2461 ttattcccgt tgcacttatt tcaatggtcg tctccttttt tgccgtagga ctcgtaggta
     2521 tgcttatcgg aaatggtttt gcaaattccg ttttatatat ctcattgccc gcaatggctg
     2581 gtggtgttgg agccggagct gtacctttat caacgattta tgcaaatgtt ttaggtacgg
     2641 acgcttcttc tatcatttcc cgcctgattc ctgcgacagc aatgacaaat gtcctagcta
     2701 tcattggtgc agcgttagtt gctcgtgcgg gacaatcaat gccaaaattc aacggtaatg
     2761 gtaaattaat ggaaaaaggc gaccttggcg atggaaaacc tagaaatgta aaaccttcca
     2821 ttccacaatt aggtgtcggt ttgatggtat cattgacttt cttcatctta ggacaaatct
     2881 gtaactattt tgttcctaaa gtccatgctt acgcattcat gatcattatc gttgttatct
     2941 gtaaaatcgc aaacctatta ccagaatatt acgaagatgc agctatcatg ttcaacaact
     3001 tgattgtcaa aaacttgaca gcagctgttc ttgccggtat cggtattgcg ttattgaatc
     3061 taaatgtatt ggcttctgct ttgacatggc aatttgttgt cttatgtttg acaagtgtta
     3121 tcgcgatttc agtagtttca ggatttgtcg gtcgtttgtt tggtttatat ccaatcgaat
     3181 caactattac tgctggctta tgcaacaact cgatgggtgg tacaggaaat gtagctgtct
     3241 tatctgcagc taatcggatg gaacttattg cttttgctca aatgggcaat cggcttggtg
     3301 gtgccatcgt tttgatcatc tcaggtttct tgatgcaatt actttcttag tcaaaaaaaa
     3361 tacctgatca tcaagtatcg gcttgatgac caggtatttt ttgataaaaa tttacaggat
     3421 tttcaaaaat cttttttgca ttttatcgga atgataaccg atgcgttcat aaaatcggtg
     3481 tgcttctact ctgtattctg cagaattaag tcttattgct gatattcctc gtcttttcgc
     3541 ttcttctacg ccagtcatca aggcctttcc gattcctctt ttttcagcat gtttagacac
     3601 agcaagtgcc ataatattga acatcggatc gctgaaaatc gattcatagg actccgcttg
     3661 tacatagcca gctgcttttt tagaagtagg atcttcatag accaagaaaa aatttttttc
     3721 tggctccttc aacagtttct tcatttgtct caccgtaacg gttaatggta catcatatcc
     3781 taattgttcc gcattgattt ctcgtagtgc ttctgcatct cctaaacgca tttcccgcaa
     3841 catccaaatc accagccaat ttttattttt ttaaatgaat ttttttatca cagtatactc
     3901 cttttttcac ttaaaaaaag acttttcagt gattttgact tgcttgaatc cagatccttc
     3961 taatgtttgc aagatgacga aagcattgta aagattgatc aaaagaacca cgatgacaat
     4021 cgcttgaaaa ataaaggtca ctttttttgg agacaaaacc ctgctaaatc ttgctcccca
     4081 caaacctcca ataacagcag cggggataac ataaaacaac atagataaat caaagcgcat
     4141 aaagctactt gttaaagcaa tcgttaccaa ttttgccagt tgagaaaaga aaatcgtaga
     4201 tagagaatat agtgttgctt cttttattgg caatgcaaac atcagcatca acaaagaaac
     4261 attgactggt ccaccaccaa tacctaaaaa actagctaaa aagcccagaa cccccccaca
     4321 tataaggtac caagtccatg acgtcaaatg aaattttggc tgatgatgct tggtgtagaa
     4381 gaacgcaaaa accaaggtca ataccgtcaa aaaaatttgg atcagctgca catgcttctc
     4441 gttttcaaat aactgtaaaa aaacttcgaa tacaatgttg ccgactactc ctccaacaac
     4501 agctccacta gatacccaac cgacaatctg ccaattcagt gatttcccgc ttgatacttg
     4561 tcgcacagtc gaaacaatgg acatcgtgaa taccgcaact gttgagtaaa aagaaatccc
     4621 tgcaacagaa tgggcgccga taagatcaag aatcggtttg atcaacacgc ctccacccat
     4681 cccggaaata gcgccaactg tgttcgccag aacgatcacc acaaaataaa tcagaccaat
     4741 catcttgtat ttcctttctc ttgtttatat cttttagcga ttgttactct tttcttttat
     4801 tatacagtaa aagaaacaaa cgaggcttcg aaagcttttc tactttttaa ggaaatttag
     4861 tgctaaacga aaaaaccggg ctctactttt cagagagtcc agcttttctt acaccttatc
     4921 gattcttttg ttctattctt cttccgccat gaatgtattg aatacttctt cgatcatatc
     4981 ccattctgcg tctgtttcga tttgttctaa ctcgccttct gttccatctt cgttttcgat
     5041 gtatgcataa gcttgtaatt ctacatcttc atcttctgaa acaccagctg gatataaaag
     5101 aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt agcaagattt cgtataatgt
     5161 ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt ccttcgtgtt cgtgggaatg
     5221 gttatgttct gtcataagat tacctctttt ctttttatta ttaaaataat tgcttacctg
     5281 agccgtctaa ataattttgt aaaatcatca cagctgctaa tttatcgatg actttttttc
     5341 gtttcgctct tgaagtattt gcctgctcta ctagcatacg ttctgcctga acagtggtta
     5401 gtcgttcatc ttgataatca accggcaagg agaatagttc tttgatcttc tctccatatg
     5461 acatggaagc ttctgccctt ggtccgatgg aattgttcat attcttaggt aatccaacta
     5521 caaaacggtc tacttcgtac tgcgccacta attccttcaa ccgatcaaaa ccaaactctt
     5581 cttctgcctc attgattcga atgatttcta ctccttgagc tgtccagcca aagggatcgc
     5641 tcacagcaac gcctactgtc ttagaaccga catccaagcc cattactctc ataaattcac
     5701 tccatgattt gagaggtagt ctttgacaat tacttccatg atttcatcgc gttcgtgacg
     5761 gcgaatcaaa tttcgtgcat cacgataacg aggaatgtag gccgggtctc cagaaagcaa
     5821 gtagccgaca atctgattga ttggattgta gcctttttct tccaaagccc gataaacaat
     5881 cgctaatgtt tcgctcactt cttttttgcg actatcgtca aaaccaaaac gtacggtttc
     5941 atctgtaaaa cccatgattc tacacctctt tcccgcaagg taattccagc ttaccttcta
     6001 cacccttatt ttactcgaag aaagggaaaa ctacaaacaa atattccaaa cttctgaata
     6061 aattaagaaa cacgatttgt ctgatttttc cttttttctg tggctttttc aaaagccctc
     6121 tttccataaa gtatacgcta tatttctttg aaagtatgtt tttacacaat tttttcataa
     6181 agttcataaa aaattcgaat tgctgctact aatttttcgt tttcgttgct tgccctgtct
     6241 cataattgat ggtgtagcca tcttgaacat tgtagataaa gacattgaat tcaatttttt
     6301 tgtcttcaat gctttctgct tccaactgca cacctcttgc taccagttca tttccctcga
     6361 aatgaggagt gacacgatag cgtacatgat gatttgtttc cttgatatat gccgcaatat
     6421 cattttcgtg ggcaagcatg tacggagcat tcaacgaacg tgtccctgtc attaagtttt
     6481 taatgttatt attttgacca gtcaactgga aaccaatcaa atggctgcgg ttgtataacc
     6541 attgtccgtc acttaatttc ttttgtttcc aacctgtcgg atcaatatac aaaggttctc
     6601 tcttttcagt aggaaacaag tcttttccca acatcgcatt ggcttgtcct actctgttca
     6661 atttgtctat atttgaatag ctttcccatg tccctttgga aagactaaga tcctcttttg
     6721 taaactccgg ctgattccca ttgatttcta tttcttgatg ttttccatca tattctggca
     6781 catccgctac ttgagaagct tgcggtgtgg aatcaaaaat ggttgtaatt tgttggaaga
     6841 tatctgaatc tttttgtgtg gtggaaatac cgagtgcccc tgcaatcaca gcagcgacga
     6901 tccccacgat cgccttggtt gtttttttgc tcatttcttt actcctttac taacagttcc
     6961 ctaaatctta acatatgttc ccctataaat aaatactgga aagaaataga aacagtaaaa
     7021 aaataaaacc agtttttaga cggcttaaga atacaaaaaa agagacggat tcatcgtctc
     7081 tactcaatca caaaacacta tttacaaggt atttattatc attcaaaaaa tttttcttaa
     7141 tactcaaaac taaacggctt ctgtacgacc tcttggtaaa cagtgttcaa aagcttgcat
     7201 ctgcggtaat aagtccaacc aagtcaaaaa cataagaagc ttttttgctt gactattctt
     7261 attacttgat gcagggcagc tttttcacag cctcttttaa gcgggtgacg ggaatcgaac
     7321 ccgcgacatc agcttgggaa gctgggattt taccactaaa ctacacccgc gatacaaaaa
     7381 cagtatacgc ctctttcact ccttcctcaa gtttttttat tctttacgct tccgatatgt
     7441 tttgtagtaa aataaaacta atcaatcaat tattggagaa gatgctatga cgggaaaaga
     7501 attagatctg ttgctggaca cttgtttact cgctgggaaa atcatgatgg aaagcaatgc
     7561 ggagatgtat cgggtagaag atacgatgag tcgaattgct ttagcttctg gaaatttccg
     7621 tttggtcagc tatgtcactc aaaccggtct ctttattggt cttgatcgta cctcgaccat
     7681 ccgaatggaa caaattacca accgttcgat taatttagaa aaagtagtaa aagtaaatga
     7741 tctttcacgt aaatatgttg cttcagagct aacattagaa gaactttact gcgaactgca
     7801 agcaatcgac cgagaccgtt ctttctttcc tgtctggttg caaatcgtta gcgcggcagt
     7861 gatcagtgga acaattatgg tgttatttgg cggaaagatg accgatttac ctgtgacttt
     7921 agtcattgga ggattgggat acgcagctta tctatatagt ttgaaatttt ttagaatcaa
     7981 atttttatcg gaatttttat cttctttatt gattggcata gccgctatct acagcgtacg
     8041 ttttggctta ggaagcaatc aggatctaat cattatcgga tgtgtgatgc ctttagttcc
     8101 tggagttcaa attacgaacg ctatccgtga tttgctagct ggacattatg tctcaggagt
     8161 ttctcgcgga acagaagcga tgatgactgc tacgatgatc ggttttgcga ttgcttttat
     8221 tttccagctt ttttattagg aaaggaacac aagtatgggg acgatattta ttcagttttc
     8281 ttttagtttt ttagcgactg cggcatttgc cgttattaca aatgtaccta ggcgttcatt
     8341 gatttgttgt ggtctttctg gtgcttttgg ctggatgatc tactggatct gtgttcaaat
     8401 aggcggatct gctgcttttg gttcattgct tggttcttta ggagtagcat tcatcagcga
     8461 tcttttctct aaacgtttga aaatgcccgt aacgattttc aatattcccg ggatggttcc
     8521 tctagtccct ggtggtttag cttatcaagc ggttcgaaat ctcgttactg aatcctatcg
     8581 agaagcggct cattatgcgg tccaagcaat tatgattgct ggagcaatcg ctttaggtct
     8641 tgtattatca gaagtcttta accataacat ccgtaatttc aaagtaaagc gtgacgcaat
     8701 catgatgaaa cgaaaaaaac ggctttttaa ataaacgaag tgtaacaaag aaaaggttgt
     8761 gaaagagacg ttcagccttg agcgatgaca aaatatctca caaacattgc gacaagtaac
     8821 cgcaggagca ataagaagta attatgagta ttaaaaaaag ttttgtaaaa atagcttctc
     8881 acatttttac ataaaattga cttaatactg agaaactgac ttttgaacac cctctatctg
     8941 taaaaaaggg gctgcgacat gatttttgtc acggcccctc ttctttattc aaattttttc
     9001 caaaacgttc gttgataata aacgatccat agagcggaaa gtattagaac taaaccgatc
     9061 acaggaatat tcactaacca aaatggataa aacaaagcca acattccgta taagacgata
     9121 agaataatac tgatgatcaa tgaacccatc atagttgcga ctaaacccaa acttcctgaa
     9181 cctctgttaa ataattggtt aatattggtc cagttcagca gcagcaaccg ataatctcta
     9241 gcgaaataac gtagtgacaa taagtagcag ccgaataaag aaccaaatag caagctaggg
     9301 ataaaaaaca aaggcagctg aaaagacagt ccgcttaata atgcgatccc accagttaaa
     9361 aaactctgca atatccatcc gaatctgaac ttttctttta aatattggtt catagagatt
     9421 ggcaaagaac ggataaacag aaaattttct tgatccaaag aaataagatt gctgataaaa
     9481 gaagtctgat taactgttaa gaaagccaat gcgatcccta ccaggaaaac aacaccgatt
     9541 aacttattat ctaaatcagt taattttatt tcgcctccta gggcaaaagc aagaataaaa
     9601 atgattggag ttaacaagga agaagaaaga acttgcataa tcaaattagg ttcttttact
     9661 aattgagcat tgtaactaaa taaaagctga tgcacatttt ggttcttttt atgggttctt
     9721 tttattttga actcactgtt tgaatcagtc agttgctctg taagctttgg tactatcagc
     9781 tgttttacag caaggaaaaa tcccagagcc gtgatagcta gtattcccca acccaataaa
     9841 ccttgttgag aaagtggctg actggcagca tcatagaatg gcaataatat cgaaatcgga
     9901 tgccgatctg ttaactcatt ttcaatagtc cccgtttgat gattcatcca catgattcca
     9961 gcaaatgcga tcagcatcga gctgccaagc aagatactag aaactgtttt tctatgtttt
    10021 ttaaacactg ctgttcgagc caaaccaaaa acaatcaaac tagccagaca aaaaagaata
    10081 atcaaaaata gagaaaacaa aaggattgag ataatgatag taagcaaaat ccctagatca
    10141 gctcgccatc cggttaaaat aaacatgact agaagcggaa aaacaaaagg aatgatggtc
    10201 aaagcaacta ccaaaatctt tgctgaaaaa acctgttctt gtccaaaagg cagaggcaaa
    10261 aatacttgca ggtcatttcc ttcaaaaaag atattataaa tgactgatat actttgagtt
    10321 aaacctaaca taccaaaaag tgccacgtaa taagtaaaga aacctggcaa tttaacaaag
    10381 ttcatagcaa acatagcaaa tccgtaaatc agtaaaaaaa tcagccctga aaaaagattt
    10441 tgagacaaaa gatagatagt taatcgcttt cccttttttc ctttctctct tgctttattc
    10501 gtgagctgtg gattaacata taaaagatta actcgaatta attctaataa ttgtcgctta
    10561 tacatacggt ccatctcctg tcgcttgcct tccagccatt ttcaaataaa tcttttctag
    10621 cgtttcatca ggtgagccat tcaataattc actaactgcc ccctgataaa tcaattcacc
    10681 tttcttcaaa atagccagtt tatcgcataa ctgttgtgcg gtatccaata catgtgtaga
    10741 aaaaatgact gtttttcctt ttgcggcatg agccttcatc atttctttca aatcaaatgc
    10801 tgcttgagga tcaagtcctt gcaaaggttc atccaatacc caaatatctg gatctggcag
    10861 caacgtgccg atcaaaatcg ttttttgacg cattccatgt gaaaaacttg cgattgtttc
    10921 attttgatga ctatacatat caaaaagcgc tgtcaattct gctaaacgct tttctttttt
    10981 ttgtgtgtct aattcatagg cagctgaaat caagtcccaa tattctccag ccgtcaattg
    11041 taaaaaaata tctggggaat caggcacata accgattttt tgtttgatac tcaatcgatt
    11101 ttccgtcaat ttcattccat ctactaaaat cgttcccgat gatggttcaa taatactaac
    11161 taaactttta atcgtagttg attttccggc accattatgt cctaaaaatc caaaaatttc
    11221 cccttgtctt atcgagagag acaagtcttt taatgcttgt tttttcccgt aacttttact
    11281 tacattgatg aattcaatca tttctgtact ccttttgttt gttattactt tttattgtac
    11341 ccttcgttat tggtggagta aatgtcaaaa agagtgcata acaaaaaagg atagaaacga
    11401 ctaggctgct acttgggttg ttctcttcta caggaaaaca ctcttctaac aggtaagata
    11461 tgagtctttg ttttctttaa aatgattcat tttctatctt ttcttgcata aaattcttta
    11521 gtttatcaat acttctcttt cccatctcta gttgttctcc atttgcaaat gttactattt
    11581 cgtttactct gctaatagat gtaatgtttt cagtattaat gatataagct tttaaatctt
    11641 tacataaata ctcatattcg agtgattttt tatatttttt aattgtgccg tttacgagaa
    11701 tctcttcatc tttcaatact aggcctattt tatttttata acctggcatg ctcattagat
    11761 aaaggatttc atcgtaatgg atatagtgat cgctattacc ataacggaca gttgtccacc
    11821 tttttttcgt agcatgttga acaggatagt tttctttttc tttcaataag taactttgga
    11881 tgcttttttt atttttttca taagtgaatt cttttatcaa ataaccacta ggcgtaatct
    11941 ttcgattaat aacagttatt gctttattcg gatgagacgt tagataaaca attttacact
    12001 tttcattctt ttctctaagc atttcgccta gttcgatacc gctataatag gcgttcaaat
    12061 cgatgtctag gaaaaaaata tctgaggaat agatttcatt atttttaatg aattcaacaa
    12121 attgtataac gttcaatccc tgaacgatct caaatttcca aggacttaat aaaattctgg
    12181 attcttccaa aatttctact agcatttttt caaaaaccaa actatcatcc aaaataaata
    12241 tacgcgttac cataaaaagt acctccactc gatattttac tatcaaaatc atttcataca
    12301 acttttcaac atacatgtta ggaataaatt gtaattctct tcacaatctt ttccaacacc
    12361 tttgaaaccg atatcaaata ctttcaggat gctgcttcta aaataaatat caaacaaatg
    12421 aatacatcat gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct gtctgagcta
    12481 tgtttattta ttttgttctt ttaaaaagaa cgatacacat actgattttt cggagcagag
    12541 actttctcag cgtttgtaac aactacagat gggatttcac gcttgaatgg ttgataatag
    12601 tctgattgga tggttccttc tattttatac cattcatcat ttttaaccgt tactccttcc
    12661 ggcatatgcg tcaacaaacc aaagactcct gaatctgcca cacaatggat aatgccgaat
    12721 cggaacaaga agatatctgt attgctatcg ttcggtgttt ggtaaacaaa tccttcatag
    12781 ctgatttttt tgccaatgaa ttcgctaggg taattataaa ttagctccat aacttctaaa
    12841 tagttttcat ctgtgatctt gatagtttgc ttatccccat acttttcttt caactcattc
    12901 atttggtcta agtagtctga tttgttgaaa taaatactgg tatccggctt taagtattgt
    12961 gtctgcatct ccggatcccc tactgattct ttacttactg ggaaattaaa tcctttggct
    13021 tctacgatcg tagtgtcgag actaactgtt ggaaacaacg tccccacgat tactggcaaa
    13081 gctagcagta cataagcgat tccacgttga taaggttttg acaacccatg gtcatgatcg
    13141 tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc acaaatacag ctgaactaaa
    13201 gccaaaagag cagataaaat cattgaaaga atagctaagt agcgataatg gacattgata
    13261 tattgatcga gtttgccaga aacttgcaag tacatcatca attcgaaata cccagataaa
    13321 atcaaaaacc gcagcatatt ttccctccta aataaataat gtgaatccaa taacgctaag
    13381 ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag
    13441 tagatttttg atatcgacca ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc
    13501 gaacagactt aacaaagaag atcctataaa cgcatctgct tctgagcaaa gagacatcgt
    13561 aaaggccaat aacagcatga tgataatggc taaaatcttt gttgagccga gcgttagcat
    13621 aattctagtt ggtaaataag tctgcatcgc agctgcgatt aatccgccaa tgatcaaata
    13681 acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag tttgatggat
    13741 cagctgattt tttggttcat tcttctcact ttgttcttct tcatggacat gatgatggtg
    13801 attttctgct actttagacg tgattttttc tttcaataca gcgtcttttt gaaaataggc
    13861 aagccagaca ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc agcttgccat
    13921 ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc acgattggat tgatgatcgg
    13981 agcagtcagc ataaaagcaa acgctgtgtg aactggtacg ccttttttaa caaattgatg
    14041 aacgatcggt acgatgccgc attcacatga aggaaagaaa aatcctaaga tcgaaccagt
    14101 aaaaatagag aagatacgat tttcaggaag ccatctcttc acacgttctg gggtcaaaaa
    14161 tacttgtaag gcgcctgaga taagacagcc taataatacg aaaggtaatg cttcaataac
    14221 gatcgataag aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag aaaacatgag
    14281 tttcctcctt tttatttcta gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    15721 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    15781 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    15841 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    15901 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    15961 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    16021 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    16081 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    16141 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    16201 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    16261 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    16321 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    16381 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    16441 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    16501 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    16561 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    16621 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    16681 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    16741 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    16801 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    16861 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    16921 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    16981 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    17041 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    17101 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    17161 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    17221 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    17281 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    17341 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    17401 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    17461 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    17521 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    17581 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    17641 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    17701 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    17761 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    17821 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    17881 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    17941 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    18001 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    18061 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    18121 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    18181 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    18241 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    18301 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    18361 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    18421 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    18481 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    18541 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    18601 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    18661 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    18721 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    18781 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    18841 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    18901 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    18961 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    19021 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    19081 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    19141 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    19201 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    19261 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    19321 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    19381 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    19441 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    19501 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    19561 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    19621 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    19681 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    19741 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    19801 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    19861 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    19921 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    19981 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    20041 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    20101 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    20161 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    20221 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    20281 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    20341 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    20401 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    20461 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    20521 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    20581 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    20641 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    20701 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    20761 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    20821 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    20881 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    20941 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    21001 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    21061 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    21121 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    21181 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    21241 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    21301 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    21361 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    21421 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    21481 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    21541 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    21601 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    21661 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    21721 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    21781 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    21841 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    21901 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    21961 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    22021 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    22081 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    22141 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    22201 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    22261 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    22321 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    22381 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    22441 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    22501 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    22561 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    22621 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    22681 tggctgtagc tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    22741 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    22801 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    22861 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    22921 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    22981 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    23041 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    23101 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    23161 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    23221 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    23281 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    23341 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    23401 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    23461 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    23521 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    23581 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    23641 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    23701 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    23761 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    23821 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    23881 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    23941 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    24001 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    24061 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    24121 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    24181 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    24241 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    24301 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    24361 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    24421 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    24481 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    24541 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    24601 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    24661 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    24721 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    24781 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    24841 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    24901 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    24961 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    25021 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    25081 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    25141 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    25201 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    25261 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    25321 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    25381 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    25441 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    25501 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    25561 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    25621 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    25681 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    25741 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    25801 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    25861 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    25921 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    25981 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    26041 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    26101 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    26161 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    26221 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    26281 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    26341 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    26401 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    26461 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    26521 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    26581 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    26641 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    26701 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    26761 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    26821 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    26881 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    26941 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    27001 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    27061 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    27121 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    27181 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    27241 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    27301 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    27361 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    27421 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    27481 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    27541 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    27601 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    27661 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    27721 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    27781 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    27841 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    27901 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    27961 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    28021 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    28081 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    28141 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    28201 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    28261 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    28321 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    28381 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    28441 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    28501 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    28561 gtattaaata atttaatttt tttgttccta tttatttaa
//
LOCUS       DAFBQW010000027        26194 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFBQW010000027 DAFBQW010000000
VERSION     DAFBQW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.028"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(149..970)
                     /locus_tag="LK889_001546"
     CDS             complement(149..970)
                     /locus_tag="LK889_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5095784.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1067..1345)
                     /locus_tag="LK889_001547"
     CDS             complement(1067..1345)
                     /locus_tag="LK889_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095785.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1613..3622
                     /gene="metG"
                     /locus_tag="LK889_001548"
     CDS             1613..3622
                     /gene="metG"
                     /locus_tag="LK889_001548"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK5095786.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(4101..4688)
                     /locus_tag="LK889_001549"
     CDS             complement(4101..4688)
                     /locus_tag="LK889_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095787.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            4863..6185
                     /locus_tag="LK889_001550"
     CDS             4863..6185
                     /locus_tag="LK889_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5095788.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6340..7116
                     /locus_tag="LK889_001551"
     CDS             6340..7116
                     /locus_tag="LK889_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK5095789.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            7109..7678
                     /gene="rnmV"
                     /locus_tag="LK889_001552"
     CDS             7109..7678
                     /gene="rnmV"
                     /locus_tag="LK889_001552"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBK5095790.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            7671..8555
                     /gene="rsmA"
                     /locus_tag="LK889_001553"
     CDS             7671..8555
                     /gene="rsmA"
                     /locus_tag="LK889_001553"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK5095791.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            9006..9635
                     /locus_tag="LK889_001554"
     CDS             9006..9635
                     /locus_tag="LK889_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095792.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(9759..10868)
                     /gene="ugpC"
                     /locus_tag="LK889_001555"
     CDS             complement(9759..10868)
                     /gene="ugpC"
                     /locus_tag="LK889_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBK5095793.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            11077..11499
                     /gene="mgsA"
                     /locus_tag="LK889_001556"
     CDS             11077..11499
                     /gene="mgsA"
                     /locus_tag="LK889_001556"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK5095794.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            11822..12358
                     /locus_tag="LK889_001557"
     CDS             11822..12358
                     /locus_tag="LK889_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK5095795.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(12398..14161)
                     /locus_tag="LK889_001558"
     CDS             complement(12398..14161)
                     /locus_tag="LK889_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095796.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(14164..15879)
                     /locus_tag="LK889_001559"
     CDS             complement(14164..15879)
                     /locus_tag="LK889_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095797.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(16658..16882)
                     /locus_tag="LK889_001560"
     CDS             complement(16658..16882)
                     /locus_tag="LK889_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095798.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            17073..19850
                     /locus_tag="LK889_001561"
     CDS             17073..19850
                     /locus_tag="LK889_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5095799.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            19931..20350
                     /locus_tag="LK889_001562"
     CDS             19931..20350
                     /locus_tag="LK889_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBK5095800.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            20362..20832
                     /locus_tag="LK889_001563"
     CDS             20362..20832
                     /locus_tag="LK889_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5095801.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            20849..21628
                     /locus_tag="LK889_001564"
     CDS             20849..21628
                     /locus_tag="LK889_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5095802.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            21615..22436
                     /locus_tag="LK889_001565"
     CDS             21615..22436
                     /locus_tag="LK889_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5095803.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            22453..23502
                     /locus_tag="LK889_001566"
     CDS             22453..23502
                     /locus_tag="LK889_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5095804.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYIQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            23515..24519
                     /locus_tag="LK889_001567"
     CDS             23515..24519
                     /locus_tag="LK889_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5095805.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            25152..26171
                     /locus_tag="LK889_001568"
     CDS             25152..26171
                     /locus_tag="LK889_001568"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK5095806.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         8504 a         4612 c         5333 g         7745 t
ORIGIN      
        1 gttcgtacga gctttttctg ctattgttaa ttttttattc agaaagttta cgaatacatc
       61 aaaattctga aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt ttcttgaatg
      121 attaggtctg aatcttttgc tcacaacctt attcatggtg ccccggcttg agctgataga
      181 tttttgaagg acggccgatt cctacaggac gttcaccgat ctcatcgaaa tattggagca
      241 tagctttctt aaaattggaa tgatcgatcg ttttataatc gatccctaag aatttagcaa
      301 atacttttcg agcttccgta atcgtgaaat ctttacctaa aacttgtaga acttgcggct
      361 cgtgttccat tttattgact acacggttga atgctttcaa gatgatttga ctatggtcaa
      421 aagccaaagt atctttgcca gaagactctc cagtgactaa atcaagcgag atagccactt
      481 ctccgctaga taaatggatc ttgttactat gacgttctag tgtgaaccag cgtacttcct
      541 tggcatcatc tccggcgatt agcggttctt ctccaataaa ggctaagtag ctgactgtga
      601 cgacccagcc tcgcggatct cgattaggcg tactgaatgt atgcagttgc tcaatatttt
      661 cttttgaaat aaccacacca gtttcttcct ttgtttctct taagacgctt tctccagttg
      721 actcatcctt ttggacaaag ccaccaggca atgcccatga atttcgataa ggatgccctt
      781 ttctttggat caacaaaact ttcagctgat cttcttcttt gttatagcac atcagtacga
      841 tatcgacagt caaagagggc ttgtcatatt ctggacgttc ctgtttttga taccagttaa
      901 gaaattcttc ttcacttgct ttatgttcat aatataattt ttcttcttct ttagaagcaa
      961 aacggttcat ctgaccacct ccattgacag taaaagtact ttttacctta ttgtagcaga
     1021 ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt tatcatttaa atatgaaaaa
     1081 taaatggaag tatgagattg aataaaaaga aaagcagata aaacggcaat actttatacc
     1141 gctcttcacg aatggcaaag aagccacaaa taacgatata tacgtcaatg ataaaacgca
     1201 aaatccaagc tgctgtcata aaactgttca aatgcccatg gaggttaaaa taaccataca
     1261 tacgaatata aaaatagctg aatattaaat gtgctaatga aagaaaagca atactaagtg
     1321 taaaaaaaag atgtttcttc tgcatgattg ttccagtccc ttactaagat agatagtctt
     1381 tattatacca cgtgtgaggg gagaaataaa aagccgtttt ttagtagata tgaaacaaaa
     1441 caaaaacccg catttattca acctattcat ccttttaaag gatagattga acgtagatag
     1501 aagtcgttta tccagtcttt ttcattttga aaaaaagcta acagcatgct ttttttatgg
     1561 tatactagca aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc ccgtggcaga
     1621 aaaggaaact ttttatatca ctacgccaat ctattatcca agcggcaaac ttcatatcgg
     1681 caactcgtat actacgattg catgtgacgc aattgctcgt tataaacggt tgatgggctt
     1741 tgatgtgttt tatctcactg gagtggatga acatggtcaa aaaatcgaaa caaaagcaga
     1801 agaactaggt gtaaaaccgc aagaatatgt agataaaatg gcggctgatg tcaagaaatt
     1861 atggaaaacg cttgatatca gttatgataa attcattcgc acgacagatg attaccataa
     1921 agcagctgtt caaaaaatat ttgatcgttt attagaacag ggagatattt acttaggtga
     1981 atatgaaggc tggtactctg tttctgacga agaattcttt acagaaacac aacttgcaga
     2041 agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg gacacgaagt
     2101 agaattggta aaagaagaat catacttctt ccgcatgagc aaatacgcag accgtctatt
     2161 ggaatactat gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa acgaaatgat
     2221 caacaacttt atcaaaccag gattagagga tcttgcagtt tcacgaacaa cttttacttg
     2281 gggagtacca gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg atgcattatc
     2341 caattatatt actgcactag gatatggctc cgaagatgaa agtctattcg aaaaatactg
     2401 gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc tttcatacga tctattggcc
     2461 aatcatgctg atggcccttg atttgcctct acctaaaaaa gtatttgggc atggctggct
     2521 attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc ctgaaatgct
     2581 tgtggaacgt tatggtttag atgcactccg ttattatttg cttcgtgctg ttccgtttgg
     2641 gtcagatggt gtctttacgc cagaagattt tgtttcccgt gtcaattatg atctggcaaa
     2701 tgatctaggg aacttattga atcgaacgat tgctatgatc aacaaatatt gtgatggact
     2761 cgttcctgac tatgcctcac ttgtcactcc atttgatagt gaactgtcta cgacggctgc
     2821 taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg gaattcaata cagcattggc
     2881 tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag aaccatgggt
     2941 attagcaaaa gacgaagaaa agaaagctga attgaatagt gtcatgatcc atttagctga
     3001 atctttgcgt atcgcagcga tattacttca gccaatcatg acagaaacgc cagagaagat
     3061 cttcaatcaa ctaggattgg actctgaaac aatgaatctt gaaggattac actttggcga
     3121 atttccttca ggaacaaaag tagttgcaaa aggtacacca atcttcccac gtttagatat
     3181 ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa caaacgaaga
     3241 tacagtcaaa tgggatccag aagaaacaga acttgtttca acgaaagaaa aacaaatcaa
     3301 atttgatgta tttgaaaaag tagaactaaa agtagcagaa gtcatcaact gtcaaaaagt
     3361 agaaggggca gataagttgc tgcaattccg actagatgct ggagacagcc aagaccgtca
     3421 aatcttgtca ggaatcgctg aattttatcc tgatccaagc gaattgatcg ggaaaaaagt
     3481 cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc aaggaatgat
     3541 cctttctgca gaagcaccag atggctcttt acaagtaatc gaagcaccaa aatccatgcc
     3601 aaatggctca gaaatcgcat agaaattcaa agggggtatg acactagttg tgtcataccc
     3661 cctttttccg aaatttgaga agcaattttc gtaaattcct tcttattact tgaagccaat
     3721 catataatac agaaacagag aactatccaa aataaatcct agaaaaagaa gtacgagctt
     3781 cgctgatttt tgttaattta gaataatact aataggaatc gtaaaaacag aaaaaaagca
     3841 taatgataaa gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct
     3901 tacattcata aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag
     3961 cgttgatgtg ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat
     4021 gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc
     4081 tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa
     4141 cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc
     4201 gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac
     4261 cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt
     4321 gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc
     4381 tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc
     4441 ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg
     4501 atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca
     4561 acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct
     4621 ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta
     4681 aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa
     4741 agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa
     4801 attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg
     4861 gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg
     4921 gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt
     4981 ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat
     5041 tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg
     5101 aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact
     5161 ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac
     5221 aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt
     5281 tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat
     5341 taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc
     5401 agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt
     5461 tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa
     5521 tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag
     5581 tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg
     5641 gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc
     5701 tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg
     5761 ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga
     5821 caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag
     5881 cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga
     5941 tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg
     6001 aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg
     6061 gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta
     6121 taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag
     6181 agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc
     6241 tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt
     6301 gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc
     6361 catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa
     6421 atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg
     6481 caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct
     6541 ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt
     6601 gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa
     6661 gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt
     6721 catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat
     6781 atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac
     6841 ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa
     6901 gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg
     6961 gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa
     7021 aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat
     7081 gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt
     7141 cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga
     7201 aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac
     7261 tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat
     7321 tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa
     7381 gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa
     7441 aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct
     7501 tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga
     7561 cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg
     7621 tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat
     7681 atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat
     7741 ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag
     7801 aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat
     7861 tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt
     7921 tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag
     7981 atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt
     8041 taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg
     8101 aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta
     8161 tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata
     8221 tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag
     8281 attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa
     8341 aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata
     8401 acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg
     8461 aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg
     8521 cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt
     8581 gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt
     8641 ttcgtttgtt tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg
     8701 ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat
     8761 gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac
     8821 aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc
     8881 ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag
     8941 ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag
     9001 aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg
     9061 gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg
     9121 cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg
     9181 cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat
     9241 tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg
     9301 ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga
     9361 tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta
     9421 atatgcctga aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga
     9481 ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac
     9541 tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt
     9601 cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc
     9661 tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg
     9721 caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt
     9781 tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg
     9841 cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt
     9901 gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg
     9961 gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg
    10021 aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga
    10081 atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag
    10141 aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa
    10201 acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca
    10261 gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc
    10321 tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat
    10381 aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca
    10441 cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct
    10501 aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat
    10561 ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg
    10621 aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata
    10681 tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt
    10741 ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa
    10801 tcagttacag aataattttc tgcattgtca tattttttat atacgttttt cagagccatt
    10861 tctaccataa tacgttccac actttctttt ttagttttat taactattta aagtataaat
    10921 gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat
    10981 gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa
    11041 ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat
    11101 gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa
    11161 catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg
    11221 atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg
    11281 gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa
    11341 ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat
    11401 atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga
    11461 atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat
    11521 aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata
    11581 taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat
    11641 gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat
    11701 aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc
    11761 ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa
    11821 aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt
    11881 gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac
    11941 atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc
    12001 agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt
    12061 tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat
    12121 cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt
    12181 gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc
    12241 gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt
    12301 aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa
    12361 aacaagcagt cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt
    12421 gatagagccg tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga
    12481 caatagctcc ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat
    12541 gggcaatcac aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt
    12601 tctctgtcaa tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac
    12661 tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt
    12721 gaccgccaat cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag
    12781 aggcttttag ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat
    12841 tttctcgcaa tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac
    12901 gtaatgtatc acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg
    12961 ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc
    13021 ctactatagc aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct
    13081 gcttggggtc ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg
    13141 gttccaaggc ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa
    13201 atgctcgttc tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga
    13261 taaatggttt agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga
    13321 ttgacgaacc actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa
    13381 ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc
    13441 ctttttcata tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct
    13501 gaaaagcttt cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc
    13561 caataatcct ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca
    13621 caaggaaaat aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca
    13681 tcataaaaat aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg
    13741 cagaaattgc aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt
    13801 gggagttttg atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc
    13861 ttaattttgc aacggataag taagctaacc gattgcttaa cacttggatg agccattgac
    13921 tgactacgtt gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga
    13981 agcggacctg tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca
    14041 caactgttgc aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat
    14101 aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca
    14161 taactattct tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa
    14221 ttcttcatgg gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc
    14281 ttcttgaata gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg
    14341 tagcgcctta cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa
    14401 aatcagtaat gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc
    14461 gccagagaaa tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg
    14521 gacaaattct tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc
    14581 tttcttaccc cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg
    14641 aacaagagca atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc
    14701 cattataact gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt
    14761 tttcccgctt ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt
    14821 aatattttgg agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc
    14881 gataattgct tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt
    14941 ttctgtgtct agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac
    15001 gacgaggttt gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc
    15061 aagaacttgt ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa
    15121 acagatgatc ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt
    15181 ggaaacacgt aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc
    15241 tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg
    15301 ttcattgagc tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat
    15361 gataaaaaat aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc
    15421 ccagtcgatc acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa
    15481 taaacgaatg accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt
    15541 gatcaaggta tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt
    15601 gattagctga ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg
    15661 gcaaatgata gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc
    15721 aatgattttt cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa
    15781 aggcaggaca agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg
    15841 cttacgatag tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat
    15901 ttgtccacaa ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa
    15961 agagaacaag ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata
    16021 gactattttt tttaaaaaaa agaaaatgag aaaacatgcc agtctcttca ttcataggaa
    16081 cgtctatttt acttaatttt tctataaatg aattctgcat aaaaaatata tcaagtaaat
    16141 gtttatagat gaaactctat ttacttcttt tttaatttgt tcttgcaatc ttttttgcta
    16201 cgtaattttt gtaaaaaagt gggttgagag aaatctaatt gattttcaaa gaaggagtat
    16261 gaaaatgaga aaaatattta ttcgtttatt gtgggtacta tcttagagga ataaataata
    16321 gtggagaaaa ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc
    16381 ccgacatcaa gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg
    16441 taggaacatc aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg
    16501 tacgtaatca ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata
    16561 actcgttttt cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat
    16621 agctaaaaac agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat
    16681 agaacgcaaa aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat
    16741 ctacgccaat aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa
    16801 ctacagcgag ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt
    16861 ttttatattt atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa
    16921 attccagtat aaaaactgga aggaactgta cgttttttag aataaaaatt ataagggata
    16981 agtaggctgc tgtctataat aagatatatt tttcaattct ttattaaaga ttttattgac
    17041 aaaatgaatc cgttttctat gatggaggag gggtgaataa gttgttgaag aaggaaattt
    17101 tatgttactt agagaaccag acagcctttt tcgatttaga acatatgaac gaggtcttta
    17161 cagcacaaag gttagctgat cattttgggg taaaaagaaa cacagtcagt cattatttga
    17221 accagttaaa tgatgagcaa ctactagtca aaatcaattc tcgaccagtg atttatttcc
    17281 acaaagaagc ttttgaaaaa caatttttta agttacgcac gaatttttat ttttctatcg
    17341 atgatctcaa agaggaacag ccttttttcg cgcaaccaaa agacttattc tcttttatga
    17401 tcggatacga cgccagtttg aaagaaagta tcgaacaaat caaaacagct ctttactatc
    17461 cagatggcgg attaccactt ttgattacag gcgaaagtgg cacaggtaaa agttatttag
    17521 ttaatttggt ctatcagtat tgtctgcttc atgatctgct agaagattct gcaccattca
    17581 tcacttttaa ttgtgcacaa tatgcagata atcctgaact tttgactagc aatttgtttg
    17641 gacatgttaa gggagcgtac actggtgcag aagaaaataa aaaaggggct tttgaagcag
    17701 cagatggtgg gatcttgttt ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa
    17761 aactgttcac ctatcttgat caaggtgtga tttatcgtat gggagaacct aatttgtcac
    17821 gccggatcaa aacacgtctc ttttttgcta cgactgaaga gattactagt catttcttaa
    17881 caactttcat tcgaagaata cctatccgaa tcgaactacc tgcacttaat cagcgtagtc
    17941 gtaatgaacg gctagaactg atttactctt tttttctcga agaacaaaga aaaatgggga
    18001 gccctttgtc agtcagtgga ccagctttgt cacttttaac aagtcagaaa ttatccggaa
    18061 acataggaga gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat gctgaacagc
    18121 gtgaacagcc tgaaatccat gtcacgatcc atcatttgca aaaagaacta cttgcgcagc
    18181 ctattactaa aaatacagca cagcaaggaa tctatattac gcaagaccag accttagagc
    18241 aattaatgga gaagcaacag cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc
    18301 taattgattt caagaaaaat aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag
    18361 aagtgattca attatttgat tttttgctgt ttgagaccag tagacaagaa aaacacgagc
    18421 tgctggttta ttggacacaa tctatccgag agacctttcg acaaatggaa actgcctatc
    18481 aaatcaaatt cgatggaaac agtgtctatg ccttgagtta ctatctctat cagcgaagca
    18541 ctatcaaatg gttaccggaa gaacatgata ttatccaact aatcaaagaa ttagaacaac
    18601 aagtttctca gtcttatcca tctagttatc actatgtcca acgaattctg gaattaagta
    18661 agatcaaatt agatattgaa gacacaagta tggatcggat tttactgacg atctatctaa
    18721 aaaaagcgaa atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct gctcacggtt
    18781 atgcaactgc tagcagtatg gcaaatgtag taaatcgttt attacaagag gatctatttg
    18841 aatcatttga tatgccacta gatgtcacac cacagcagat tgcttcagaa atccttgatt
    18901 attgcgaaaa cagcgatgtt tcaaatggat tgatcatatt agtagacatg ggatctctga
    18961 aagaaatcca tcagtttttt aaaaaacagt tatccgtacc gcttttgatt ttgaataatg
    19021 tgacgacacc tttagcaatc acagtagggg aatgcctgca aaaaaatgta tcattagaag
    19081 agatagcaga agagactgtt cgacagatcc agccagaatg gcgactgctc tatccagagg
    19141 aaaacaaacc caaagcactc attactactt gcttcactgg aatcggtacg gcggtccact
    19201 taagtgaact gctagaaaaa agcttaccta cggcttgtca gttgaaaatc atcccatatg
    19261 aatatcagca attaaaagat aagaaaaaca gtgacccatt attttccata tacgaagtcg
    19321 taggaatgat tggcaccaca gatcctgata tcaccaatat cgactatctt tcattagaag
    19381 acctcatctc gggggaaaag atgtctgtac tagctgaatg gttggacact actatgaatg
    19441 cttcagagaa ggaaatattc aatcagcgca tcattcgtaa tttttcattg gaaaaagtac
    19501 tggattccgt gactattcta gatacagaga aggtgatggg agaaatcgat cattttatga
    19561 gagaattaga ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg
    19621 tccatgtgag ctgtctcatt gaacgattga tccgacaaat gccaatcgaa acctatcaag
    19681 gattagagaa atggaagcag tgtcaaaaag aagggttatc tgctattaaa tctgcgttta
    19741 gtgtcattga agagcattat agtgtcatga tccccaattc tgagattgcc tatatctatg
    19801 atattttgag tgaagataca gagtttctct cgatagaaga agaattttga acataagcaa
    19861 gttgttgcca ctaattttgg catgcaactt gcttttttag tatgtgtaag tgaaaagagg
    19921 agatgaaaaa gtggatagag aaattatctt agcttcccat ggaaaatttg cttcagggat
    19981 attagattca ttagagctga tctatgggaa aaatcattct attactgtat tcgattgcta
    20041 tactgatgag aattttgatt tagcggaaac agtaaggcag ctttttatga ggtacaaaga
    20101 taaagaaatc attgttctga cggatctttt tggcggaagt gtcaacaacg aattcttaca
    20161 atatatcaat cgggatcatg tctatctcgt tgccggattg aatttgccac tattgattga
    20221 gctagtatct cggttggacg ctgaagtaga gacaactgtt ttgatccagc aggtgctaac
    20281 tgattcaaaa aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa tagaagaaga
    20341 cgaattttag gaggaagaaa aatgattgta ttgacaagag tcgaccatcg gttattacac
    20401 ggacaagtag ctttttcatg gacacaaaca ataggcgcag actgtatttt gattgctaat
    20461 gatgatgtac ctacgaatga gatacgaaag acaacgatca aattagccaa gccacaagga
    20521 gtcaaactag tgatcaaatc aattgatgac tcgattgccg cgttgaaaag cggggtgaca
    20581 gataaatata agttattcat cgtagtggaa tcgatcgagg atgcatataa gctggcagaa
    20641 gcgtatccag aaatcaaaac catcaactta ggagggatca aggcaaaacc tggtactcgc
    20701 agcatcggaa aagcggtaaa tattttacca gaagaggagg aacgcttgaa acgattaatt
    20761 gacaaacaaa tagaagtaga aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga
    20821 gaagttttgt aagaaaggaa gaacctcaat gattgtacaa gcagtcttat taggaattgt
    20881 tgcatttgtc gcacaatcag aatatgcact agggacatcg ttgctttctc gtccgattgt
    20941 gacggggctg tttacaggaa tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc
    21001 aactttagag ttagcattta tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt
    21061 tgtgaccggt gggattttag ggacggcttt tgctattaca gctggagccg gtacagaaac
    21121 cgcgctttta ctaggtttgc cgattgctac gttgacattg atattgaaaa atatttactt
    21181 agggcttttg attccaatta tgaaccataa agcagacaat tatgcagaag aaggaaatta
    21241 taaaggagtc gagcatatgc acttgcttgc cggtatcggt ctttccttga tgcttggtgt
    21301 tgtggtaatg gtttcttata tggtgggaag caatacgatc ggaaatctat taaatatgat
    21361 tcctgacttc gttcaaaagg ggttgtcagt agctacaggg ttgattccag ctcttggttt
    21421 tgcgatgctt gctcgtttgc tgatcaacaa acaagttgca ccatattttt tcttaggttt
    21481 tgctatagct tcttacttag agattcctgt gaccggagta gctatttttg gtgcgatttt
    21541 agcagttgtg gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg
    21601 ggaggtagaa gaagatgaag aattctgaga tcaaaaacag caaaataacg aaaaaagagc
    21661 taaatcacgt attttggcgt tcttttcaaa tggagttttc ttggaattat gaacgtcaga
    21721 tgaatatggc ttatgtttat gcaatgattc cgattttgaa aaagttatat cagtcgaaag
    21781 aagaaatggc agcagctttg aaacggcatc tagagttttt taacacgacc ccacatatcg
    21841 ttacactgat tttaggaatc acggctgcaa tggaggaaga aaataaggat gatcctgaat
    21901 ttgatgtgac tgcgatcgat agtatcaaaa catctttgat gggacctctt gcagggttag
    21961 gagattcttt cttttgggga actttgcgat tgattgccac aggtgtgggt acctctttag
    22021 ccatgcaagg aaatatttta ggccctatct tgttcatttt gattttcaat attccgcaca
    22081 ttctgtttcg ctatctagca acaggttggg gatatcgctt aggaacgggt tttttgaaaa
    22141 agatccaggc aaacggtatg atggaaagtt tgacattagg cgcgtctatt attggactga
    22201 tggtcgttgg agcaatgact gccacgatga tcaccatcaa tattccttta aaaatcggtt
    22261 caggagaaaa tgctgtgact gttcaaagta tttttgatga tatcgttcca aatatcttat
    22321 gtcttggagc atttggcata gtgttttatc tattgaagaa agaagtgaaa ccattaacga
    22381 tcttgatcgg actagctttc tttggaattt ttggttcgtg gatagggatt ttttaaaaaa
    22441 ggagcagaga aaatgtttac gatgcaagat tatatctatg aagaaaagga agtactaagt
    22501 acgatcttga aaaagaatga tttttcgaca agagaacata tgaaaaaaac agtgaattta
    22561 ctgatcttag caacgggttc atcttataac gcttgtttag cagcgaagcc agcattggaa
    22621 tcttatggtg atcttactgt cgatattcaa gaaccttttt attttttcaa ttatggaaaa
    22681 ctttctcctt caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact
    22741 gttgaagcag taaaaatgat acaaaaacaa gggttacctg ttgttgctat cacaaatgac
    22801 gtgcaaagtc cacttgcttt agaagctgca caaattattg atttaggtgc cggggtagaa
    22861 tcagtaggct tcgttacaaa aggctattct gcgactgttt tacagttact actcttaggg
    22921 ataggaattg gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga ctacatacaa
    22981 cagttgcgca aaataatcaa ccatttacca gcgattattc aaaaaacgga agaatttttt
    23041 gatggatatc aaagtttatt tcgtttagca cagcgtttcg tcaccattgg atatggtcca
    23101 aactggggaa cagctaaaga agcagagacg aaattgactg aaacaatccg cgttccgtca
    23161 caaggatttg agttagaagc ttatatgcac ggcccatacc tggaggcaga tgcttcgcat
    23221 ttgctattct ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga
    23281 tatatgagtc ggtacgttgg agagactctc accattacca caaaaaaagc aagaaacgag
    23341 aaaacacttg gattagcaat agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt
    23401 ccgtttcaac tattcgctta taaaaccgca gtagctaaag gtattgactt gaacaaaaag
    23461 atttttgaag actttgatac agtcttgaaa agtaaattat aagaggtgga aatcatgctg
    23521 aaattcaatg aagaagaaca aatcaaagca ataaaaggcg cactagcttt acgtccgcaa
    23581 gtagaagaaa tcatcgataa actttatgcg gaaaaatttg acgctgtcta ttatctagga
    23641 atcggtggaa cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac
    23701 ttgcctgtct ttgtacagca tgctgcagaa tattacacta ctggaaacaa aagattaaca
    23761 aaagattcta tcgttatttt gtcctctgtt accggtacaa ctcaagaagt agtgaaagca
    23821 gtagaacaaa tcaagaaagt aggagcgaca ctgattggat ttatcgataa agcgaacagt
    23881 aagttgtctc agctatgcga ttttgtggtc acttatccag ctcctggaac agaacaaatc
    23941 aaatttttta tggcagcaga tcgattgatg tatttgaatg gagaattcga agcttattca
    24001 gaatactatg aacagctcga acgttattta ccaacagggt tagtggaagc agagaaaaaa
    24061 gcagatgcat ttggcttatc atttgcagaa aaacatcgac gtgatagtat gcattatttt
    24121 attggagcag ggaatcaatg gggagctgtt tattcatatg ccatgtgtta ctgggaagaa
    24181 caaagctggc ttccatcgaa atctatccat gctgccgagt ttcttcacgg gacgttagag
    24241 atcgtagaag aaacaacccc agtcacatta tttttaggcg aagatgaaca gagagtgtta
    24301 tctgaacgtg tggctaaact tttgccaaaa atttgcagca attatacatt gatagacaca
    24361 aaagactatc cagtagaagg tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta
    24421 ttgatgcatg tagtgacaca acgcatcgat gctcatgtgg agcagttgaa ctgccatcca
    24481 ttagagatac gccgctacta tcgtcagttt gattattagg atagactgga taaaaagaat
    24541 ataaaaaata aagagagcag cgataactgc tccctttatt tttttaaaat attggcatca
    24601 attacgttat aaaacacatt ttttgcatta tctacagttc aacttataat ctgtttaatg
    24661 ggacatgtgg gtgccaacca ttttttgtta tgcaatagta ctatttagtt gttgcatgga
    24721 tccgtagtaa aagtccaagt acaacgagtt gcgccagttt acatagcgtt ttgtatccag
    24781 aatgttgact aaaacaaaag ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc
    24841 aggacctgac agatttagaa atgttcaatc tgtctcttat gtattttaaa tcaatactca
    24901 ctaggagaag tttcctaacc tcctaatagt aatgctggta gtaacattgt caaaactgct
    24961 tcctcttagg aacaagcaaa gcataacgag tgtaaaaata aaatgtagac tattgtaaca
    25021 tatcattgat tatttcaagt aaaaacgata aaagaagtaa acaaacactt aaaaacaatg
    25081 gaaattagtc ccaactttaa gaaatgtata ctataatgga aattatcaat tatttatttg
    25141 gaggaatatc catgaaacaa aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt
    25201 tagcacaaac attggcagaa aacgggcatg atgtccgtat ttggggaaat gtacctgaac
    25261 agattgatga aatcaataag tatcatacaa atcagcattt tttacctgat ttgacgatcc
    25321 cagaatcgat tatcggatat aaagaattgg cagaagcagt ggataacgca gatgctattt
    25381 tatttgttgt tccaacaaaa gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga
    25441 atactaagcc aatcattata catgcaagta aaggactaga acaagatacg cataaacgaa
    25501 tctcagaagt tttggctgaa gaaattccag aagaaaaaag acaggcaatt gttgtattat
    25561 ctggtcctag ccatgctgag gaagtggcag tacatgatat cacaacaatt acagctgcaa
    25621 gtattgatca aaaagcagca gcctatgtac aaaaactgtt catgaatgat tatttcagga
    25681 tttatacaaa taatgatgtg attggcgtag aaactggtgc agcactgaaa aatatcattg
    25741 caattggtgc cggcgctatc catggattag gattcggaga tgatgcaaaa gcagcaatca
    25801 tgactcgagg attggcagaa atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga
    25861 catttatcgg tttgagtggt gtgggggact tgatcgttac ttgtacaagt gttcattctc
    25921 gcaattggcg tgccggtaac ttgttaggac agggacataa gctggaagaa gttctagaaa
    25981 acatgggaat ggtcgttgag ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac
    26041 agctaggtgt tgagatgcca attactcaaa caatctataa tgttttgtat aatggagaag
    26101 atatcaaaaa agcagcaaaa gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt
    26161 ctcttcgtta aatagatgat caatagttaa tcgt
//
LOCUS       DAFBQW010000028        26090 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFBQW010000028 DAFBQW010000000
VERSION     DAFBQW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.029"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            126..3653
                     /locus_tag="LK889_001569"
     CDS             126..3653
                     /locus_tag="LK889_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBK5095807.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="LK889_001570"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="LK889_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBK5095808.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="LK889_001571"
     CDS             7441..7782
                     /locus_tag="LK889_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5095809.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="LK889_001572"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="LK889_001572"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK5095810.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="LK889_001573"
     CDS             9139..11559
                     /locus_tag="LK889_001573"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK5095811.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="LK889_001574"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="LK889_001574"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK5095812.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="LK889_001575"
     CDS             12993..13730
                     /locus_tag="LK889_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5095813.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="LK889_001576"
     CDS             13744..14559
                     /locus_tag="LK889_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK5095814.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="LK889_001577"
     CDS             14572..15219
                     /locus_tag="LK889_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5095815.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="LK889_001578"
     CDS             15235..15891
                     /locus_tag="LK889_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5095816.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="LK889_001579"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="LK889_001579"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK5095817.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="LK889_001580"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="LK889_001580"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK5095818.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="LK889_001581"
     CDS             18233..18751
                     /locus_tag="LK889_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5095819.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="LK889_001582"
     CDS             18858..19358
                     /locus_tag="LK889_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5095820.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19638..20558)
                     /locus_tag="LK889_001583"
     CDS             complement(19638..20558)
                     /locus_tag="LK889_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBK5095821.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20723..21736
                     /locus_tag="LK889_001584"
     CDS             20723..21736
                     /locus_tag="LK889_001584"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBK5095822.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="LK889_001585"
     CDS             complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="LK889_001585"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK5095823.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22821..23261
                     /gene="fabZ"
                     /locus_tag="LK889_001586"
     CDS             22821..23261
                     /gene="fabZ"
                     /locus_tag="LK889_001586"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK5095824.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23376..24137)
                     /locus_tag="LK889_001587"
     CDS             complement(23376..24137)
                     /locus_tag="LK889_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5095825.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24326..25420
                     /locus_tag="LK889_001588"
     CDS             24326..25420
                     /locus_tag="LK889_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5095826.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25506..>26090)
                     /locus_tag="LK889_001589"
     CDS             complement(25506..>26090)
                     /locus_tag="LK889_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5095827.1"
                     /translation="GKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIV
                     GIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKE
                     FSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRKVNQTFISSV
                     SNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8623 a         4523 c         5498 g         7446 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
    19501 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    19561 tagagcgtgg gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt
    19621 tgcatcgttt gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca
    19681 gaagctgtca attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt
    19741 ccgttcaatg cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat
    19801 ccaaagatag aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt
    19861 actcgatcaa tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct
    19921 aaaagtactt gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg
    19981 atgaaatgga cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg
    20041 acggttcctt caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt
    20101 tccatgataa taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg
    20161 attgccacac ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca
    20221 gataaaattg catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga
    20281 ctatgtaaag tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa
    20341 gacttatcaa tcagatattt tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa
    20401 atcagatata aacttttttg gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc
    20461 caaaaccgct gaaacgcatt tactttgctg acagcttgat ctgtcaatgt ctcgccagaa
    20521 gagtctgtcg taccggcagt tgctaataat gaaaacataa aatcctcttt tctaaaattt
    20581 gattctttct gtattgtacc aagaagtttt caaaaaagga aatagcaaaa ataactataa
    20641 tgaaggaaaa aagctgaaaa taaaatgaca aataatatgg ataatgataa aatgtggaga
    20701 aaataacgaa ggtggtaagt atatgcaaat cgattggaac aatctaggtt tttcttatat
    20761 caaaactcct tggcgtttta ttgccaaatg gaaagacgga caatgggaag aaggagaatt
    20821 gacagaagat aattacctga cgattcatga aggctctcca gcgcttcatt atggtcagca
    20881 atgttttgaa ggtctaaaag cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc
    20941 tgaacaaaat agtcgacgga tgaatcaaag tgctagacga ttgctgatgc caatggtccc
    21001 tgaaaagatg ttcgttgatg ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc
    21061 gccctatgga acaggcgcta cattatactt gcgcccttta ctgattgggg tcggtgaaaa
    21121 cattggtgtc catccagctc cagaatattt atttgtcgtc ttctgtacgc cagtaggagc
    21181 gtatttcaaa ggtggactaa aaccaacgaa ttttattgta tctgattacg atcgtgctgc
    21241 tcctaagggg actggtgcag caaaagtcgg cggaaattac gcagcgagtt tacttccagg
    21301 aacagaagca aaagaactgg attactcaga ttgtgtttat cttgatccag ccacacatac
    21361 gaaaatcgaa gaagtaggtg cagcaaattt ctttggaatt acaaaagacg gaaaattcat
    21421 cactccgcaa tcgccctcta tcttaccaag tatcacgaaa tattccttgt tgcagttagc
    21481 tgaagaacga ctaggactgc aagcaattga aggagatatt ttcattgatg atctagatca
    21541 atttgcagaa gctggtgcat gtggtacagc ggctgttatt tctccaatcg gtgggattta
    21601 tcacaataac aatcttcatg tattttatag tgaaacggaa gtaggaccag taacaaaacg
    21661 gttgtatgat gaattgacag gtattcaatt tggagacata gaagcaccag aaggctggat
    21721 tgaagtcgtg gaataatttc aaagtaatga gttgaaaaaa acgtaaaaag aaaaaatcag
    21781 gaagagactt taaaaagttt tttcctgatt tttatatgtt ttactttaaa gaatgcaaca
    21841 aaagtttatg aaagatggac acctttgtca acgtagataa tatcaccgat gattccgctg
    21901 gaaagtgggc tgattaagaa agcacaagca ttaccgactt cttcgatcgt cacaccttgc
    21961 tgatcaggcg tacgagattc agataactgg atcagttttt gatagtcttt aacacctgtg
    22021 acagctaatg ttttgattgc cccagctgag attccattga cccgaattcc tttaggcgag
    22081 aactcagctg ccaaataacg gatagcagct tcaagagaag ctttcgcaat acccatcatg
    22141 ttataattcg ggattgcgcg ttctgaacct agataagtca tgctaacgat cccgctgttt
    22201 ggtgcaagaa acggttgtgc gtattttgat acagcaatta aagagtagct gctgatatct
    22261 tgagctaatt gatagccgtc tctggaaata tcagacacat ttccggacaa ttcttctttg
    22321 ttggcgtaag caactgcatg gacaaggccg tggatctccc cagcatattc accgatggca
    22381 ttcattgctt tttcgatgct ttcgtcgcta gaaacatcac attctacagt gaatgcttct
    22441 tcgccgacca atttgactaa tgattttttc attcgttcat tttggtaagt atagataatt
    22501 tctgcccctt gtttcaacat tgcttcagca caaccccaag caatgctttt tttgttagcg
    22561 acgcccataa tgacgatttt ttttccttct agaaatgaca taatgactct cctttgtttc
    22621 tgataaaaat ttgatagtcg taaaaaaata gagaataata gctaaaataa cttcttgatt
    22681 tcttaattat tagtcaaaat gttttgattg tcaaactatt ttttcttaaa ttaaaaaaag
    22741 attgctaaat atttaggaat atgatacttt gatagtcaaa gtaatgaaaa tggcaattgc
    22801 ctgaaagaag gagtttaatt atgtcttacg tattatctgc tactgaagta atggaattga
    22861 ttcctaatcg ttatcctatt tgttatattg attatgtaga tactttagaa ccaggaaaaa
    22921 agatcgttgc gacaaaaaat gtaacaatca atgaagattt tttccaaggt cactttcctg
    22981 acaatccagt gatgccgggt gcgttgattc tagaaacact ggcacaagca ggttcgatac
    23041 ttatcctaaa atcagaagag tttctaggaa aaagagctta tatcggtggg atcaataagg
    23101 ccaaattccg tcaaaaagtt gttccaggag atgtgctgaa atgtcatttt gaaatcacca
    23161 aaatgaaggg atcagtcggt acagcgttat ctgcagcata tgtagatgat aaaaaagtct
    23221 gtgagtgcga gttcacgttc atcgtcaacc aaaatgcttg actataaaac ctgatatagc
    23281 aaaacaaaag gaagcttact tgctttttat gtaaaaagaa gacgcctgaa tcctagcata
    23341 cgttgtcatc tatgcaggat tcagacgtta tttttttatt tttgttttgc ttcttgcagt
    23401 atattgattt tatcgatttt tacttcttct tttggtttgt ctgaactatc tgtttcgata
    23461 tttgcaatag catccacgac atccattcct tcaatcactt ggccgaagac agtataatgg
    23521 ccatctagtg aaggataacc gccgttttta taagcgtcga tgattgcttg aggatagtcg
    23581 tcttttaaaa gaccatcatg catatcatct gtattttgaa cgataaagaa ctggctgccg
    23641 ttactgttag gatcttgtga gcgagccata gataacgcac ctcttatatt ataaaggtgg
    23701 ttgttgatct ctgtttcaaa tccttctccc caaatgcttt cccctccagt gccatcgcct
    23761 ttaggatcgc caccttggat catgaaatct ttgatgacgc gatggaaagt cagtccgttg
    23821 tagtaaccgt cttttgcatg tgtcataaag ttttcaaccg cttttggtgc gtgttcggga
    23881 aataatttga ttttgattgt gcctttattc gtcaccattt cgaccagatc ttcattttcg
    23941 gcgacatctt tcgtcaattg tggtaattcc aatgcgttca aatcgacgct actggcagtc
    24001 gtgctttccg cagtagatgt actttttgaa ctttctgtag acgaactaga agaagtcgtg
    24061 ttgtttgcag aacaaccagc agcaaacagc aatgctgctg ctaaactaat acttaaaata
    24121 actttatttt tcttcataac tgttcctttc caatttacta ttttgttatc tttaatgata
    24181 acaaaataga gggaaaatac ctacttaaat tattggaatt tatcatttat tttaaaagaa
    24241 aaagaaaagg tgtagaccat ttactttgtg tttgctataa tgtaagtgga tacaaaaacg
    24301 aaaagatgca ggaggaaatg gtaatatggg aaaattagga atctcgattt atccagaacg
    24361 gtcaacattt gaaaaggaca aagcgtactt ggatttagct cataagtatg ggtataaacg
    24421 ggtttttacc agtttgctgc agatcaatga tgataaagaa aaagttcttt cagaatttaa
    24481 agaagtagtt gattacgcga atagcctagg catggaagta atggttgata tcaatccagc
    24541 gttatttgaa cagttggaga tttcttacga tgacttatct tttttccata agatgggtgc
    24601 ctacggggtc cgcttagata tcggattcac aggagcagaa gaagcaaaaa tgacgcgcaa
    24661 tccttttggt atcaaaatcg agatcaatat gagttcgggc acgaattacg ttgacaacat
    24721 catgagttat tcgccaaaca cggataattt attaggttct cataattttt atcctcatcg
    24781 atactctggt ttaggatacg aacactttgt tttttgttca gaaaaattca gaaaatataa
    24841 tttgaatacg atggcattcg tcaattcgca atctgctgag tttggccctt ggcctacgca
    24901 agatggcttg tgcacgctgg aagaccaccg agatcttgaa attgcgacac aagtgaagca
    24961 tttaatcctt acaggattga ttgatgatat ttctattgga aatgcgtacg caagtgaaga
    25021 agaattgaaa gaaatggcag aagcatttaa cgcggattat ccaacattga aagtagatac
    25081 ggaagaaggt attacagaaa acgaacgaat ctgcttgttt gataaccttc atagttatcg
    25141 aggggatcgc tcagaatata tccttcgttc aacgatgact cgtgtatact acaaagataa
    25201 agatttcccg cctcataata cgcgagatat gcatcatggg gatgtactga ttgacaatga
    25261 agggtacggt caatacaaag gagaaacaca aatcgccttg aaggatatga aaaacgacgg
    25321 acgtgtcaat gttgtaggaa gaatctccga tgacgaatta ttcctccttg actttttgaa
    25381 accatggtca agctttaaat taattgaaaa taaaaaatag ataattagtt attgcagaaa
    25441 taatccgcta gaaatagcca gttatttctg cttttttatt ttgttttttt ataggttatg
    25501 atttgtcaaa ttaagctaga ataaaatgct tcttgtacat agctcaaaaa tatttcgatt
    25561 ggtgttctgt aattcaatga ttttcttgga atatgattac gttgattgct gacactggaa
    25621 ataaatgtct gattcacttt tctaaaatcc attgatttcg gcagtccatt acgacgcaga
    25681 atcccgttag aattctcgtt taatgggcgt tgagaaggtg ttccagggtc cgcaaaatat
    25741 atatcaatat catgttggtt actaatggct ttccagttag aaaattcttt tccacagtca
    25801 aacgtaatag atttaaagaa gtttttaggg aagcgagaaa accattgatt aagggcagtt
    25861 tcaatatcta atgccttacg gccgttgggt ttaatcgtga taatgacttt agataatctt
    25921 tcaactaaag taatgacggc acttttatga tgaatgccaa cgatcgtatc accttcaagg
    25981 tgaccaaact cagaattgaa atcaggataa ttatcaggtc gatcatggat tgagcgctga
    26041 tactgttgtt ttccccgttt ttcctgatgg ccattgggtt ttcttttacc
//
LOCUS       DAFBQW010000029        25806 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFBQW010000029 DAFBQW010000000
VERSION     DAFBQW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.030"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            6..848
                     /locus_tag="LK889_001590"
     CDS             6..848
                     /locus_tag="LK889_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5095828.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1795
                     /locus_tag="LK889_001591"
     CDS             848..1795
                     /locus_tag="LK889_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK5095829.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1810..2043
                     /locus_tag="LK889_001592"
     CDS             1810..2043
                     /locus_tag="LK889_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBK5095830.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2374..2958
                     /locus_tag="LK889_001593"
     CDS             2374..2958
                     /locus_tag="LK889_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5095831.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3089..3756
                     /locus_tag="LK889_001594"
                     /pseudo
     CDS             3089..3756
                     /locus_tag="LK889_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            3767..5182
                     /locus_tag="LK889_001595"
     CDS             3767..5182
                     /locus_tag="LK889_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5095832.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            5200..5769
                     /locus_tag="LK889_001596"
     CDS             5200..5769
                     /locus_tag="LK889_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBK5095833.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5879..7633
                     /locus_tag="LK889_001597"
     CDS             5879..7633
                     /locus_tag="LK889_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095834.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7593..9359
                     /locus_tag="LK889_001598"
     CDS             7593..9359
                     /locus_tag="LK889_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5095835.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            9882..10151
                     /gene="rpsN"
                     /locus_tag="LK889_001599"
     CDS             9882..10151
                     /gene="rpsN"
                     /locus_tag="LK889_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK5095836.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10166..10345
                     /gene="rpmF"
                     /locus_tag="LK889_001600"
     CDS             10166..10345
                     /gene="rpmF"
                     /locus_tag="LK889_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK5095837.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10377..10754
                     /locus_tag="LK889_001601"
     CDS             10377..10754
                     /locus_tag="LK889_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095838.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10775..10924
                     /gene="rpmG"
                     /locus_tag="LK889_001602"
     CDS             10775..10924
                     /gene="rpmG"
                     /locus_tag="LK889_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5095839.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10948..11931
                     /locus_tag="LK889_001603"
     CDS             10948..11931
                     /locus_tag="LK889_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK5095840.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11961..12083
                     /locus_tag="LK889_001604"
     CDS             11961..12083
                     /locus_tag="LK889_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK5095841.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12101..13624
                     /locus_tag="LK889_001605"
     CDS             12101..13624
                     /locus_tag="LK889_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK5095842.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFRDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13648..13887
                     /locus_tag="LK889_001606"
     CDS             13648..13887
                     /locus_tag="LK889_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095843.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14080..14541)
                     /locus_tag="LK889_001607"
     CDS             complement(14080..14541)
                     /locus_tag="LK889_001607"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5095844.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14736..14954)
                     /locus_tag="LK889_001608"
     CDS             complement(14736..14954)
                     /locus_tag="LK889_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095845.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15072..16688
                     /locus_tag="LK889_001609"
     CDS             15072..16688
                     /locus_tag="LK889_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK5095846.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16929..18269)
                     /locus_tag="LK889_001610"
                     /pseudo
     CDS             complement(16929..18269)
                     /locus_tag="LK889_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18545..18700)
                     /locus_tag="LK889_001611"
     CDS             complement(18545..18700)
                     /locus_tag="LK889_001611"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBK5095847.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18996..19328)
                     /locus_tag="LK889_001612"
     CDS             complement(18996..19328)
                     /locus_tag="LK889_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5095848.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19842..20033)
                     /locus_tag="LK889_001613"
     CDS             complement(19842..20033)
                     /locus_tag="LK889_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095849.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20127..20513)
                     /locus_tag="LK889_001614"
     CDS             complement(20127..20513)
                     /locus_tag="LK889_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5095850.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21207..22412
                     /locus_tag="LK889_001615"
     CDS             21207..22412
                     /locus_tag="LK889_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBK5095851.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22429..22863
                     /locus_tag="LK889_001616"
     CDS             22429..22863
                     /locus_tag="LK889_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095852.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23480..>25806
                     /gene="esp"
                     /locus_tag="LK889_001617"
     CDS             23480..>25806
                     /gene="esp"
                     /locus_tag="LK889_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBK5095853.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         8893 a         3698 c         4831 g         8384 t
ORIGIN      
        1 ataatatgct tcaaaatttt attaatggtc tgtatgactt tcacttttta cagaatgctc
       61 taattacatc tgtagttata gggattgttt ctggaacagt aggttgtttt attattttaa
      121 gaggaatgtc tcttatgggt gatgctattt ctcatgctgt tttgccagga gtagcaattt
      181 cttatatttt aggtattaat ttttttattg gtgcgattgt atttggtcta ttagcttcta
      241 tcattattac ttttataaaa agcaatagtg taataaaagg agatacagca attggtatta
      301 ctttcagttc ttttttagca ttaggagtta tccttatagg agttgcaaat agctcaacgg
      361 atttatttca tatcttattt ggaaatattt tagctgtaca agatattgat aagtggatta
      421 caattggcgt ttctgtttta gtgttggtta cagttgttct attttttaaa gaattattaa
      481 tcacttcttt tgatccatta atggcaaaag caatggggat gaatgtcagt ttttatcatt
      541 atctattaat gattctatta actttagtag cagtaacagc tatgcaaagt gtgggaacta
      601 tattaattgt tgcaatgtta attacaccag ctgctacagc atatttatat gcaaacagct
      661 taaaaactat gatatttatt tcggcgacag tcggagcgtg ctcatcattg ctaggtttgt
      721 tcattggtta tagttttaac atagcagcag gatcaagtat tgttttaaca gcggcattct
      781 tatttgtaat tagttttcta atctcaccaa aacaaaaatt tttaagaaag aaggagagaa
      841 agttatgatg agaaaatgga aagcagtact gggaagtctg gggattttga ttgctctttt
      901 tatattcggg gcatgttcaa caaatagtaa agacaaagat acagtggctt cgaacgaaaa
      961 attaaaggta gtagttacta attcgatttt agcagatatt actgaaaata tagcaaaaga
     1021 taaaattgat ttacacagta tcgtacctat tgggaaagat ccccacgaat atgaaccttt
     1081 gcctgaagat gttcaaaaaa cttcaaaagc agatttgatt ttttataacg gtgttaactt
     1141 ggaaactgga ggaaatgctt ggtttacaaa attagtaaaa aatgcgaaca aagaggaaaa
     1201 caaagactat tttgcagcaa gtgatggcat agatgttatt tacttagagg gtcagagtga
     1261 gaaagggaag gaagatcccc atgcttggtt aaatttagaa aacggtatta tttacgctaa
     1321 aaatattgaa aaacagttag cggaaaaaga tcctgataat aaaaaattct ataaagaaaa
     1381 tctagataag tatattgaaa aattggattc tctagacaaa gaagctaaat ctaaatttgc
     1441 ttcaattccg aatgataaaa aaatgattgt tacaagtgaa ggatgcttta aatatttctc
     1501 gaaagcgtat aatgtgcctt ctgcttacat ttgggaaatc aacactgaag aagaaggaac
     1561 accagatcaa ataaaacact tagttgaaaa attacgcaca acaaaagttc cctccttatt
     1621 cgtagaaagc agtgtggacg atagaccaat gaaaacagta tcaaaagata ccaatattcc
     1681 tatctattca acgattttta ctgattcaat tgcagaaaaa ggacaagatg gtgatagtta
     1741 ctatgcgatg atgaaatgga acctggataa aattgctgaa ggcctttcga aataatttgg
     1801 agggtaggta tggtacaaat taaaacgact actatactaa tttatttact agcaattcag
     1861 gaattatcaa aaagaaaaaa gaaattaaaa aataatgatt tagcaaaagt tctaagtgtt
     1921 agtccagcat ctgtaagtga gatgctggct aaacttagat atgaggacta tttagatata
     1981 ggatttcaat taactgataa agggaaaaaa tttattgacg attacaaaaa aggttttaag
     2041 taaaagactt tatttttagg gaggataaga aaatggctat tggagtgatt atgataggac
     2101 ttaccctaat agctggatat aattttttta aatctttctc ggattatttt agaaaaggtg
     2161 aatgatttag atgagtctat taattcttgt aggattgctt tttgtatcag ttattttgct
     2221 ctttctcatt agtttagctc taaggaatga gttcagtaat agacgttaac gattgaaact
     2281 tcaagtcctg tgtaagataa tatagttaat tcatcttatt aggaggaagt acccatagaa
     2341 accagagatt atattacaac tcaccaaaaa ttaatagaaa gtggactaag ccattttgaa
     2401 aaagaaggat ttcagcaaac taagataagg caactgtgct tgaccgcagg cgtaactacg
     2461 ggtgcttttt ataaacattt tacttctaag gaagcgttat ttgaagaaat tattcagccg
     2521 catattaatg cgcttattaa tatgtataat gaagagttta ccaaatacct cttaaactta
     2581 gaaaatatag atatagagaa aagttggaga gagaacttca atgaattaga tccatttatt
     2641 aattatattt ttgagaataa acgtatattc gatttggttt tattccaatc agctggaacc
     2701 aaatatcaag atattgttga aaaaattaca tcatttgtga caaaaaagac agtctgttgt
     2761 ctagataagt taaagactat cggaatgatc aatcaaaatt tagtctttaa tgaagaagaa
     2821 atccacttct acatttactc attctacgct acattctatg atatattgaa gcatagctat
     2881 tcaaaagaaa agactatgct attaaccaga agattgtatc aattttctgt tcctgggtgg
     2941 attgaattac tgagttaatt aaaaaaaagc ttgcattgag aattgttctc agttatgctt
     3001 tttgataaga tcgcactgtt atctgaataa cggtgcattt ttgtcagctt aagataacag
     3061 tgcgatctta ttttttggag gtgaaagagt gtcaaaaatc aatccgttaa ccttgattac
     3121 tttaaatatt tttttccggt cattattttt ttaggaaagg gaaatatatt tcaagtgggg
     3181 tgtttcatta ttgctacagt tttagtattg atgtttcgta aaataaagac atgtatttat
     3241 ttcatcttga tttattcagt tctgttttta gtaatgaaaa ttattgatgc tatcaagata
     3301 gagtttttta ctatattttt tgggacatcc ttatatattg tatttcgaat gttgccggtc
     3361 gtaatgatct catggttgtt ggttagtgtc tatactagca atgaattatt atcctcttta
     3421 gaaaaaatac atttaccgaa aaaaataatg ttaagtatta cagtagtgat cagattcttt
     3481 ccgacttatc gttctgaaat aagaatgatt aaagaatctt taaaaatgag aaacattcgc
     3541 ttgtcttttt ttcagcctct gcaatatttg gaatttctgt tagttcccgt tcttatacgt
     3601 gcaactatga ttgctgaaga aatgaccgct aatgcaataa caaaggggat agaatctcct
     3661 gtgaaaagaa gttcttacta taaaacaaac atgagcttat tagactatat catcctgttt
     3721 tccgttacta tttttttctt aagtattttg atttgaggtg aaagaattgt ttgaatttaa
     3781 caatgtttct ttttcatata aggatgttaa ggaacaagcg cttgttgacc ttaatttttc
     3841 aataaaaaaa ggcagtctta cattgattac tggttcttct gggagtggga aatccacact
     3901 attaaagtta atgaatggac ttattccaag actttatgac ggaaaattgg ttggggaaat
     3961 tttatttgaa aataactcgc tacaatcctt aacacaagaa agtatatcaa aaaatattgg
     4021 ctacgtaagt caaaatccta gaggacaatt ctttacaaca aatacaacga gtgagttagt
     4081 attttcgatg gaaaactttg gatttacaaa agagcatatg gataaaagaa tcaatgaatt
     4141 agctgaatta ttagatcttt caaatatatt agataaaaac attttttcgc tatcaagtgg
     4201 tgaacggcag aaaatttcta ttggatgtag tttatcgcta aactctaaat taattatatt
     4261 ggatgaaccg tcttcaaatt tggacttcaa agcgactaag aaacttgctg acttactttg
     4321 tgatttaaaa aataaaggat ttactattgt tatagctgaa catcgttttt actatttgaa
     4381 agaattaatt gatcaagttc tatttgttaa aaatagaact gtaaaaacta caactattca
     4441 gttgttaaaa actgaaaaca ataacgcatt aaggaaattt gacattttta atagttcgat
     4501 tgaagttccc tatatcgaaa aggtaagtgc agatgtatta aaagttgaag aaattactta
     4561 tgaaaatatt ctaaaagaca taaattttga tgtcaaagtt ggagatgtta ttggtttggt
     4621 agggaaaaac ggtgtgggga aaacaacctt actaagaata ctcatgaaga ttattcgccc
     4681 taccaaagga aagataatcg aaaataaaaa aaatttgtca tcgccgtttt tagtaatgca
     4741 agaaatggat taccaatttt tcacagaatc agtgaggagc gagttagccc tggggaatga
     4801 aatagttagt aaagatcagc aagaaaaaat tttaaaaaaa atggaacttt ataaaatgaa
     4861 agatcaatta ccgtttgatt tatccggtgg agaaaaacaa agattattgg tttctatagc
     4921 ttcattgtca aaaactaatt tatttctatt tgatgaacct acaagtgggt tggattatat
     4981 caatatggaa aggattgctg accttattaa agacttaaaa gaaaagggtg cagtagttat
     5041 agccactcat gatccggaat ttctttataa aacatgcaat agaattattt atttaaaaaa
     5101 tggtggaata aaaaaagata taaaacttag agtagaagat tcaactttga tagaacagat
     5161 ttttaatgac ataacgaaat agaattgagg agaatatata tgaaaacaaa agattatatt
     5221 tttattggtt tagctacagt tatttcccta gtaatttact ttttttcaat aatggttagt
     5281 agtattggtg gggcgtttgg tcacagcata tccccaggga tttttggatt attatctgga
     5341 atcatatttg tttatattag ctataactac cctagaaaag gaatttttac tatttacaca
     5401 attgttttat tgttattttt tacgttaatg ggcggggcgt acttaccttg gtttatcagt
     5461 tcgattagta tggcgatctt agctgatatt attctatctg tatttggata tgatcgagca
     5521 attcctcaag ttgtgtcttg ggttctaatg caactaggca gtgctgccgg acagtggatt
     5581 cccatatggt tctttacaga tcgttttcgt caagactgga tagatagagg acaatctgct
     5641 gcaacaatgg atgcaatgat ccactacgcg gtgggtatat ggggaattat ttcagtgtta
     5701 gttgtagcaa gtttatctat gattggtgta ttgattggaa gaaaagtctt aaaaaaatac
     5761 aaaaaataga acgtagttta ccttctatct gtttgtacat ctctaattta cgctattacg
     5821 gaaagcatta acagttgtaa acgaataaga gaagaaattg aatgcggggg aggaaaaaat
     5881 gaaagtatac aaaaaattat ttagttatgt ccctaaaatg aaatactacg gatggttagc
     5941 cactttattt tctgattttt ctgtcgtatt aacagtctat ggatattaca gtatttacaa
     6001 atttcttcag tctctaatta ttgtaggaga tgaatattta gctaagagtt atgcggttca
     6061 aactgttgct tggttaactt taggagcttt attttatatt ttttctggct tattttctca
     6121 tatccttggg tttcgcttag agactaattt gagaaaaaag ggaattagcg gtttaacaga
     6181 agcaagtttt cgtttttttg atttaaattc ttcgggatat attcgaaaga caattgatga
     6241 caacgcggct aaaacacata tggcaattgc tcacttgatt ccagatagtg ctcaagccat
     6301 agtaacacct gttttatcag ttgccttagg ctttttcatt aactttaaaa ttggattggt
     6361 tctaatcgcc atgttagtaa ttggtgtatt cttgttaaag aaaatgacag agaatacaaa
     6421 ttttattcaa aaatatcaag aatcgttaga tgtgttaagc tctgaaacgg tagaatatat
     6481 ccgcggaatt caagttataa agatatttgg tgctaaagta acatcattta aagccttgaa
     6541 taaagcaatt aacgattatg ctaagtttgc atataagtat tctttgagtg ggaaaacacc
     6601 ttttgttctt tttcaatggc tattttttgg catagtttgc attttgatag ttcctataac
     6661 cttttttata acttctctta ccactcccaa gatcttagct gttgacttgt tgatgttgtt
     6721 ttttttaagt ggaattattt ttgtttcttt catgagaata atgtatgtaa gtatgcactt
     6781 gtttgaagca agatatgcag tcgaaacact agactccctt tatttagaaa tgaagcaaga
     6841 ccaactaaaa tatgggaacg agacacagat taaaaattgt gacattgcat ttgaaaatgt
     6901 cagcttttca tacaaagaag agtgtgtttt agaaaatttc aacttagttt tagaacaaaa
     6961 aagaatttat gcattagtag gaaaatcagg aagcggaaag agtacgatag ctaaactaat
     7021 ttcaggtttc tacaatgtgg ataagggaca gattaaaatt ggtggtaagc ctttaaatca
     7081 ctattcaaaa gacacaatta ttaacacaat tgcttttgtc tttcaagata cgaaactatt
     7141 caatcagagt atttacgata acgtttctat agcaaatcct caagcgccag caagtaaagt
     7201 atttgacgcg ctaagattag ccggttgcga atcgatttta gaaaaatttc ccgaaagaga
     7261 gcatacctta attggtagta aaggagttta tctgtcagga ggagaaaaac aacgtatagc
     7321 tattgccaga gcaattttaa aagatgcaaa gattgttatt atggatgaag ccagtgcatc
     7381 tattgatcca gataatgagt atgaactaca aaaggctttt caatatttga tgaaagaaaa
     7441 aacagttatt atgatagctc atcgattatc cagcattaaa tctgtagatg aaattcttgt
     7501 cttgtcagaa ggaaaaatag tagaacgtgg aaatcataat gaattaatgg ctaaacatcg
     7561 agtgtataaa caattggtca ctaattatga gaatgcaaat gattggaggc ttgacaatga
     7621 aaaattttta taaaaaaaaa tttgctttaa ctgatcaggg cgcagaagct ttaactaaag
     7681 cttctatttc tagttttttt gtatactgca ttaatatggt accagcattc attataatga
     7741 tgctgataga cgaattagtt ttagaaaatg caaagccccg ttggctttat tttgcagtat
     7801 cgtttgttac tttgcttttc atgtattggt tgttagatag ggagtatgaa aacttatata
     7861 atagtactta taaagaaagt gcgcatttaa gagtgcaaat tgcagacgat ttgtcaaatt
     7921 taccattatc ctatttttca aaacataatt tatcagattt atctcaaact atcatgtctg
     7981 acgttgaagg tattgagcat gcgatgagtc atgcaatacc taaagccggc ggtatggctc
     8041 tgtttttccc ttttatttca gtgatgcttt tggttggtaa tgtcaaaatg ggattagctg
     8101 ttattttgcc aacgttattt agttttgtct taatcttgtt atcaaagaaa tcccaaacga
     8161 aagccaatac taaatattac gatactttga gagaaaactc ggaagaattt caagaaacta
     8221 ttgaattgca gcaagagatt aatagcttta atctatctaa aaaagttcaa gacagacttt
     8281 tcaaaaaaat ggaagagagt gaaaggattc atttaaaggt agaattaagt actttttcag
     8341 tcatggcctt atcctctatt ttctcatatg ttagtttagc agtagtcatt ctagtaggtg
     8401 ttcacttact gttaacgggt gaagtgacta tactctacgt cgttggttac ttactagccg
     8461 caataaaaat aaaggattcc ttcgattcta tgaaagaagc agtgcttgaa atattttatt
     8521 tagctcctaa aatacagcga attagagcta tgaaggaaac cagtatccaa gagggatcag
     8581 atagtcctct gaaatctttt gacattgagt tgcgagatgt ctctttctca tatgataaca
     8641 ataccccaat tttagatcat atctctttta cggctaaaca aggagaagtg actgcgttag
     8701 taggtgctag tggttccggg aaaactagta tcttgaaatt agtgtctaga ctgtatgatt
     8761 atgatgaagg ctgtatttta attgatgggt atgatattaa acgtgtatct ccagcttctt
     8821 tgtttagcaa aatcgctatt gtttttcaag aggtaacttt gtttaataca agtattttgg
     8881 aaaatatacg tattggaaat agtcaagcgt cagatgagga agtgaaaaaa gcagctcgtt
     8941 tagctaactg cgaagacttt attgaaaaac tacctgacgg ttatcataca ttggtcggag
     9001 aaaacggaag ttcactttct ggaggagagc gacaacgcct atccatagca cgggcttttt
     9061 tgaaaaatgc gccaatttta attttagatg aaattactgc cagtttagat gcggaaaatg
     9121 aaaaacgtat tcaggaaagt ttaaatcgtt taatacaaga taagacagta ctaattatat
     9181 cgcatcgctt gaagtctatt gaaaaggtaa ataaaattgt agtgatggat caaggaaaac
     9241 tagttgatca aggtactcat aatgagttgt atcgaaggtc agaaatatac aaaaatctca
     9301 taaagaaaac taaactatct gagaaattcg tctatgaaaa agaagctcaa tcacgatgat
     9361 gaaataggct ttaaattaac ctacaatttt tgggatatag ttataattct ttttcaaaaa
     9421 gaataatttt attaaacagc atctaatttt gaatacacga aagaatcagt atgattgaat
     9481 tagttcactt aatgtctatt cttataatat aaggaataat caaagtttta aaaaattggc
     9541 gataaaccag ttaaacataa aagattgaat aaaaagttat agtttctttc atattgccgt
     9601 tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt tgattggact aataaaagtg
     9661 aattcttatt tttcagagcc taaactgcat attcttttaa atgatttgat tatctctatg
     9721 taccgataaa ttcgttttca aatagttatc tattcatatg agttttattt catgttattt
     9781 attgttgaca agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga
     9841 tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa
     9901 ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat
     9961 taaaagccgc aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc
    10021 gtctgaggct tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta
    10081 tgtcccgtat taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag
    10141 caagttggta aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa
    10201 gaaacggtta agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc
    10261 gaatggtgat tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa
    10321 agtaattaaa ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg
    10381 aaattattta cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc
    10441 aatcgctaga taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa
    10501 cgggtttacc gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc
    10561 aaggcttatc atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg
    10621 agttatttca atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag
    10681 tttcacaatt ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc
    10741 ttgaagatcg ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg
    10801 tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg
    10861 gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta aagaagtgaa
    10921 atagctaagt ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt
    10981 ttaggggctg gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg
    11041 gaagaaatta ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta
    11101 gttcatagcg aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc
    11161 agtgatttat tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca
    11221 atttcacaaa tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca
    11281 cttttaacag cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt
    11341 gatagtgaca gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg
    11401 gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta
    11461 cagagagaaa tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa
    11521 acgattcaaa aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttgaa
    11581 agtgattcat cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat
    11641 gagtttaaat caattctctt aacttcagat caagacataa aaaaagattt tttactccaa
    11701 tggattgact ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt
    11761 gctttacaag agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt
    11821 caatacacca ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat
    11881 gaagaaagga tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta
    11941 gtgattgggg gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta
    12001 aaagcccgaa tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa
    12061 ataaaatgaa gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg
    12121 acttattggg atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa
    12181 agtatcgaat gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga
    12241 tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc
    12301 agaacctcat gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt
    12361 ttttgtatat cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa
    12421 gaaagctggg cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga
    12481 agaagaagat catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg
    12541 ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt
    12601 gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa
    12661 acggttggat gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac
    12721 gcaacacgca gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc
    12781 tggactaagt cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt
    12841 taaaaaaaat aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa
    12901 aactttggcc aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac
    12961 aaaagaacaa atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc
    13021 gttagaaaaa acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga
    13081 tgaaaagaca gttgctaatg ggtactttag agatgccgat gtgaaggatc gcgaattgag
    13141 tgattataca ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga
    13201 agttttcgat tacaaagcaa aaatcaataa agacatgaca gctgccgagt acaaagatta
    13261 ttatactact ggctataaga cagatattga tactatcaac attaaagaca acaccattga
    13321 ttttgtagtc aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt
    13381 gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc
    13441 tggtcgtttt aaatatattc aatttagtga tcatggaatt gcaccaagta aggcagagca
    13501 tttccatatt ttctttggtg gagaaagtca agaaaaactc tataatgaat tgcataattg
    13561 gcctactttc tatccagctt cattgtctaa acatgaaatt gctcaagaaa tgatggctca
    13621 ttaaaattca atttttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc
    13681 aagaatattg atgcttatcg gcgttatcga tttgcaaagc aaatgagatt aaatgggagt
    13741 acattagctg atattgctgc aactttacat gttagtattg acacagttca tagagtatta
    13801 aaacgggatc ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca
    13861 aaacggcata atagcttggc caattaattg ataataccag agtagatcat ttttccctta
    13921 tttggatttg atctgctttt tattatgtct tgaattgtca aatcaagcgc aacactttat
    13981 caaagaagac ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt
    14041 tttcaatcat ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca
    14101 agtattctcg aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg
    14161 atctggaaaa taaaagggca tattattcaa tgcttctgta actgcgaaat gattggagaa
    14221 ttcttttcct ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag
    14281 ttcaatcatt ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa
    14341 aaggaagcat gattttctgt ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc
    14401 tacaatatcg gctttccaat gaccaaaatc acttctatca tctgcggatt ttggacggcc
    14461 atggatcgaa tgagtaaggc aaatctttcc acgagactcg acatgatttt ttgtatgtct
    14521 tgtttttcct cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc
    14581 tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg ataaaactaa ttgaaaagtc
    14641 catatgttca tgcttcaaac gattcgagat ttcctctggc gaccactgaa aatcgacaaa
    14701 taaatgacga acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt
    14761 ttttcttcga taagcataat ttttttccgc tttggaaggt gaatagtttt tttcttgttt
    14821 cttctcagtt catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta
    14881 atagaaaatc cttcttcgtg aagcagaaag attctttctc gttcgagtat agtaagatga
    14941 cgatactggc tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg
    15001 gatatcgatt atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa
    15061 agaggttccc tatgaaaaaa atacctatta caatcttaac tggctatctt ggttcaggaa
    15121 aaacgatact tctaaatcat attctgtacg gtgatcacgg aaaggaaatt gctgtcattg
    15181 taaatgacat aggggaagta aatgttgatg gggtattagt cgacaagaaa ggttttaaaa
    15241 gaactggaga aaaacttgtt tcgatgccga atggatgcat ttgttgtaca ttacgcgaag
    15301 atttgattgt tgaactagat caattagtgc aattttctga cttgaatcaa attgttattg
    15361 aagcttcagg aatcagcaaa ccaattccaa ttattcaagc aattatatta gctaaaaacc
    15421 ctttagacat tgatttgacc caacgattag agattggtgc cattattata gtagtggatg
    15481 taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag
    15541 gtaatgtagt tgaagaagaa caggatattc gtggcttgtt aattgatcaa ttcgagtgtt
    15601 gtaacatatt acttttaaat aatagtgact tagtgtcaac caagacgatt tacaaaatta
    15661 acggtctaat tgctaagtta cagtccaatg ccaaaataat taggacaatt aaaggtgaag
    15721 tagacttatc gaaaattcta aatacaaatc tttttaatat agatgagact tcctattcag
    15781 tgggctggtt caaagagtta gagcttggct atcaaaatca tattcctgaa acagaagaat
    15841 atggtatctc aagttttatc tatcgaaatc ggttactcct tgacgcacaa aaatttgccc
    15901 atcttacgga taaaaatttt cctggcaata ttacaagagc aaaagggttt ctttggtttg
    15961 cccaacatag tgatttttcg acgatctttg aaacatcggg tagctataaa agtattgaat
    16021 cattaaatta ttgggtagct agtttaccta aaattgagtg tcaaatgatt atacaaaaaa
    16081 atcccgaatt gaaggaagcc tggcatccgt tgtttggtga acgcattaat aaactagttt
    16141 ttattggtat gaagatggat caacaatgta ttaaggaaca acttgagggt tgtttaactg
    16201 attctcttaa tgcagataat ttccgaaaaa tttcaccatt tccatggaaa aagtctaata
    16261 tgtttagcca gatatcgtta atgaaatttt taagaaaagc tggtgtcaag ataggcgatg
    16321 tcataattat tggcattttg attattacct cttttattcc cttatttgta ttctctatag
    16381 aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc catcgttcga attgatggca
    16441 aggaaatgga tcgcttcgat ttggataaga tcaatcataa actcgtgaat tatcatcctg
    16501 cgaagggaca atataatatt gttgagatta aagatggaag aattcgagtt aaagaagata
    16561 atagtcctga tcaaatcgcg gttagaactg gatggatctc aaaacccggt caaacgagta
    16621 tttgtttacc ccataagtta gttattagta ttgagaaaaa agaaagcaca gattactaca
    16681 tttactaatc tggaaataga tgaggaggtt tttaataaga aaaagatatt attattaata
    16741 atagcagggg ctttattaag tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca
    16801 acaggcacaa gtatctttaa cgggacctgc aatggtttag ttaacagtcg gttagattac
    16861 ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac
    16921 tcacacggct agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg
    16981 gtagtattct tagttttctt aaaatatttt cctcgatata aaaagattcg ttctcggtag
    17041 ttttgaaagt ttctatagcc ataggcaatt cgttttagta ctttgatatg gttattcaaa
    17101 cattctatag ggccgttgga atagggaacg cgcaacgctc gtttgatttc tgtttggtat
    17161 ttcttgaagg tcgtgaacac tggttggtag tgctctggca actccttgta gtcttctttt
    17221 aggagcgcga cgaattcagg aacatcttgt cttctgataa cggaaagaaa atcctggtag
    17281 acttcgtagc ctcgctttaa ggaatcatca taagataata gccgatcgac tatctctgct
    17341 tctgtcagat gatctcgaaa tgacggacgc cagtagcgat gctgaccttt caattcccac
    17401 gcattctttt gaagtagttt ccagtatttc tttaagtgct tcgaccgacg atcccctttt
    17461 ttaaaggtct tcattacttg aattcggtgg ttggtaaaag cccgaccaat gtgttggcta
    17521 atatgaaagc gatcggcgac gattcgggca ttcggaaaac actctttcac caatgtgcga
    17581 tagggtgtat agaaatctga aaccactaga cgcacttgtt cacggacttt tatagggtag
    17641 cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt ccacaatatc aatcagttga
    17701 tgggtcttgc cgtccatcat gacaaagctc atggctcccg agacattttt cactgatttg
    17761 aattcatcga aacacaccac ttctggtaac agctggctca aagaaatggt tttaggccga
    17821 aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt gcttcatacg tgcgatcaaa
    17881 gacatagaaa gaggttcgct taatagctca agaatggctt gtttgactct gcgagcaatg
    17941 gagcagcgac gatcactaac tgaatcttct gctaaaaaag tctttccaca gtgtttacat
    18001 ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt tactgacatc attcaataag
    18061 atggatactt ttttccagcc ctatttaatg atcgtttgtt cattttgata gtggcgatga
    18121 aagcattcct ccggttgata ggtcaatgtg ccatcaaaag caaaacatgt ttcgtcattg
    18181 atgcgctctt tgcgaaaggc atcctcatga aaggtaagat ttaggtctag aatatctagt
    18241 aactctttgg taaactctga ataggacatc ttgaaacact actttgaatg gtttttgtcg
    18301 actttattct aaaggatttt aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat
    18361 tggcaacaga aggcgtcacc aacactagat attatagatc ccttattttc ttcgcttcaa
    18421 attgtcaagg agtcatcata aattttgtat ggattctttt gtttgggtag tagtcttcta
    18481 aataatcttg gattaacacc ttcatctcta ttttactttt atatttgttg gagtcgatta
    18541 attctcattt gattgatcga tgaaacgctt caattactgc attgtcaaca ggagtcccag
    18601 tttggctcac tgaatgtgtg atgtggtggt cgttcaacag ctcttgataa ataaaagacc
    18661 gatgctggct acctctatcg gaatgaatta ccgtaggcaa ttctccttaa taaatttgta
    18721 atggacggac gacaatctct ttttttatgt gtgaatctac catatagtaa cgtagaatac
    18781 gagttgctaa gtcaatatag gtggacaaat agaggcgacc gtcattacat ggaatgtaag
    18841 taatatccat cacccaaaca gcatcaattt ggttctgttc aaattatcga ttcaacattt
    18901 ttttaccatt tctttgattg ttttttctct accatgagaa gatttgcgtc gatgaaatcc
    18961 tcttgtgcat aaattcattt cacgcataag aacagctaca acacgtttat tgatttcgat
    19021 tccttcatcg taaagttttt gagtgatccc tggagatccg tatcgatctt tgtgttcgta
    19081 gaacactcgt cttcctcttt tagtcgttta ttttcctgtt caagaatgta gaaatctgct
    19141 ttatcaggta atctttttcc ttgtccagca aaagcatttt tcccgtatcg acgatattct
    19201 gaaacccatc gggaaattgt tggacgagag atattgtatt cttcagcaat tttcttggta
    19261 gttacttttt tctcaaggat atcctgtgaa atctttagtt taaatttacg atcatatgtt
    19321 tttctcaata gagttcattc cttccggtaa cgtaactgat agtcaagaaa agtgtatcat
    19381 agcccaactt agtatcaggg tttgtcaact aaactgtgga agttgataga taagagtttt
    19441 taacaaccaa aattctcttg actatttttg aaatcagaca aattttgatt ggacacatag
    19501 ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt ctagaggaga
    19561 agtcctcgtt gaagtaccat agcccagttc tggattcgac ctcctgccca cttcatacgt
    19621 aaataaagta attttaggag tgagactgtg acaaaagtta tttttaagct aaaaacgaat
    19681 taaaaaacgt tgataattaa aatcattcgt tttagattct atgaatcatt atagaacgct
    19741 gtaaaatcga tactttataa ttataaagaa gatagtttca aatttattgt tcgaaatttg
    19801 aaactatctt tttacttatg tcacagactc ccttagttga gttattcttt aatttcgaat
    19861 ttcgagatag ctagatatat tgcaagcata cattcttgat aacaatcttg atcatatttt
    19921 ccagttctcc aggattgctt atgaatcaaa gagctaaatc ttttgagaat tgcctcaaca
    19981 gcttcttcat ttccagcctt tgctaaaatg aaatcttctt ttaaagaatt cataaatcta
    20041 ccttcttccc atggacttat aatctatttc tttgggaagg aggagaacga gcaaagaagc
    20101 atacaaaatt agttattatg cagatattaa accagcattt tttgaatttt tttgtaaagt
    20161 cgatgtttta aatttgtcgc accctgtctt gtaatcccca gaacctttcc aatttcttta
    20221 tcagttttat caaagataaa tttttcgata agaaaaattt gttcaggttc tttcaacaaa
    20281 ggaaggattt ttgctaattt tatatcttca acaaggagag tcacatttaa aacattaatc
    20341 ggttgtttaa attttatagt atctttagta aacaaatatg gtgggagttg atcaagttgt
    20401 tcatgttttt gaatttcttc ttgtttttta taataagtat tagcggtgtt caaaatgacc
    20461 ttttcaaaga atcgaatcaa ctgggcattt acgcgttcgt cgttatctga catattgcac
    20521 acctcctcca ttaagattta aagggaaaag aggcaaaaaa cacgaaaaga agctaatgca
    20581 attatgctgc gctgcgaata acttctataa gctcacatgc attgatagga ctgctgatgt
    20641 ttattttcaa aaatgttttt ttagttgata atggatagtt atctttatac ttagaaaaga
    20701 aaattcatat atcttcttaa atatcaaaca tgtccttatg gctaagaaaa tgtcttgagc
    20761 tttttacgta taataaatct tttctaaaac ctccttttgg ataatattct tattcgtgta
    20821 tagattgtat tttttatttc ttcccccttc ataattaaca actagaaaag agttcgaata
    20881 gacacgtatt tgaacgaaaa tttttattgg attcccaaat tggaatattt agaaacgaaa
    20941 aattcctatt atctgtatag tgcttagata ataacggatc ataataatta ttgtctttga
    21001 actagatata tgtaaatagt attcatcatg atgaaaaatg atgagttctc ggtgataaaa
    21061 tgtacttttt acggtggaat atacatgaaa gcgataagtg gatattttat aatgaagtaa
    21121 gttagcttat tttgacagag gaatagtatt ttagataacg tataaaatac tatttaatga
    21181 tgtagaaagg aaagagagaa acaaaagtgt tttcagataa agaagtctta ttaaagtctc
    21241 ttcatgtttg tttaaagtta cctatcgtgg tttttaattc cgaattcagt attataaaag
    21301 agtatcgctc agatagaact acttattttt tttatgatta taaaaagtta ctaactgata
    21361 atattaaaaa aactaaagat ttccatttct ttactggtga ttttaatgag atgtttcttc
    21421 tatacatgtt taaaaccaga tattacttat ttggtccatt tagagcaaat aatattgata
    21481 aagttttttt taaactaaaa atgaacaatt tgaatgttgc aatggctgat agagaacgac
    21541 tctataattc attgcaaaat ttgactttgt attctttagg agacattcga gatattctga
    21601 ttttagttca ttattttttt actggaaaaa tagaagatct attccatgaa ccgttaattg
    21661 aatatacaga aaatctaagt aaaactatcg agcaaatcaa aatagataat ctgttatcac
    21721 aaaattacga tccagaaata tatctgtttc tttatgaaaa taaaatactg gaatatgtta
    21781 aaaatggaga tattcgaaat ttagaaaaca tggtttttaa tctgagtaat ggaataattc
    21841 caagtgttag tggagatacg attcggtctg aaaaaaatta ttctatcatt gtttttgaaa
    21901 agttagcaca aacgtctata acattaggaa tggatataat agaagcgtat caaagtagag
    21961 atgctttgat acaagaaaac gaattagctg tatcgttacc tgaggtctta aaagtgaggg
    22021 attctgggat tgtttattat actaaggaaa tcggaaaaac taagatagag catctttctc
    22081 ccttaatttc ttctgtcgtt caattcatcg gcttaaatat ctataaaaga attactgtaa
    22141 aggaaatagc taattatttt tctgtaagtg aaactaaatt aagagaaagc tttaagaatg
    22201 aaatgcatat tacgatttat aattatatct ctaagcgaaa aatttctacg gctaaaataa
    22261 tgttgaaatc aaaccatact atcagtgaag tttctttagg tttaggattt tcagattctt
    22321 cacacttttc gagagtattc aaaaagtacg caggtgtatc tcctaagcaa tatcaattag
    22381 gtttagtgga caatttcaac aacattagct gagaggtttt attttattat gagactatat
    22441 catttacgta gtttggagga gttgaggaaa ttggatttta acaaaaaatt tttttgggaa
    22501 atttctgatt tgagttgttt cgtagaatct gagcgtctaa gtaatggtaa taatagtgtg
    22561 ttaaatgaat atgacaaagt agtaattaat aaagtaaaaa gtttatgtga agaagctaga
    22621 gaatctatca gtcatattaa tggtagcaat tttaaacaaa cattatttaa tctgattaag
    22681 ctagatgcta aaatatcaag ttatttattc tttttgatac atgatgaatt tatggattat
    22741 caaaaattgg atcagttaat tgaaagagaa agctgggaag attattacgt gggtctaact
    22801 ttttcagaaa agttaaacaa ctacaagtta atagattata agtatttgag tgaaagcaat
    22861 taattattca tgtagagtac tagacacttt aaatatggac aattcaatta taaagaaaaa
    22921 gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt tgtttcgcta aaaacgagtg
    22981 aaaaaatgtt gataactcca atcattcgtt taagattcta ggaatcattc agaaatgctg
    23041 tgaaaccgat actttataat tataaagaag gtgatttcaa attccgaaga aggaatttga
    23101 agtcaccttt ttacttatgt cacagtctca ttcagtaatg cgggataagg ctccttattc
    23161 cgttatttga atactctgaa gtttaatcta agaaagggta atattttgaa aacatgcttc
    23221 atcgcataga aatgaactta atttcagaaa gaatacatac atatcttttt atatgttttc
    23281 agctgctgaa tggttaaaaa gcacaaatat ccgaaaaaaa catgaagaaa tgtacttttt
    23341 ttgttgtaat gttcatgaaa ttctctaagt aaattagtta acataaggtt agtaagattc
    23401 ctattctgtg aataatacgt atcagattta tcttttattc ttactaggaa agtaattatt
    23461 aagggagttg atttgaataa tggttagcaa gaataataag agagtatttc tagaaaaaac
    23521 aaagaaacga gtattgaagt acagtatcaa aaaattaagt gttggggttg catctgtttt
    23581 gattggtgtt ggtcttgtct tgggaacaac tgaactagtt aaagcacaag atgaaatttc
    23641 tccaagcagc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca aagtagcttc
    23701 ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt cggatttaca
    23761 atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg gatttgaagt
    23821 ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc ttcaattaga
    23881 tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag gaattggatc
    23941 ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa atatttggaa
    24001 ggtcaattat attcgtgcaa gtgatggatt atttgctggg gcagaaacaa ccgatacgca
    24061 aaccgctcct aatggggtaa ttacgtttga aaagagccta gatgaaattt ttaaagagat
    24121 aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg taagtcatca
    24181 agacgatgat aaaattgtac cgggtattga tagtacgggc tactttttaa cagattcaga
    24241 tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta aacatggatc
    24301 agtaagtgca aaatatgaga aacctaatac tcaaacaaaa gatggttcag gatccacagg
    24361 tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt tctcatattc
    24421 atcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac gattagttcc
    24481 atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat atgatgtttc
    24541 gtttgaatcc ggaacaaaag tagctgattt agcaatcgag cgacgggaag gtcatgaaaa
    24601 ttatggcata ggttcaataa ctaataatga tttaactaaa cttatcgatt ttgctaatgc
    24661 aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat tagatgaaat
    24721 cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg gtgaagattt
    24781 catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca cttatggaac
    24841 aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata aagaagagag
    24901 cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg atgttgacac
    24961 aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca aagcccaact
    25021 tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg aaaaagatgg
    25081 ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa gtaaattaat
    25141 cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac cagaagaagg
    25201 ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg tagttggatt
    25261 taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag atgcagagta
    25321 ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag gtattgtaac
    25381 tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa aagcttctca
    25441 agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa cgacagttca
    25501 aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg ccttttcaat
    25561 ttcagatgtc tataataagg acttcggtaa tgcaagtgtt gatgcaaaca ctggtattgt
    25621 tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa caattcctat
    25681 taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa ctgtaagtaa
    25741 agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac
    25801 cgcagg
//
LOCUS       DAFBQW010000030        24170 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFBQW010000030 DAFBQW010000000
VERSION     DAFBQW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.031"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            160..627
                     /locus_tag="LK889_001618"
     CDS             160..627
                     /locus_tag="LK889_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBK5095854.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            902..1138
                     /locus_tag="LK889_001619"
     CDS             902..1138
                     /locus_tag="LK889_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095855.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            1162..1503
                     /locus_tag="LK889_001620"
     CDS             1162..1503
                     /locus_tag="LK889_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095856.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(1496..1687)
                     /locus_tag="LK889_001621"
     CDS             complement(1496..1687)
                     /locus_tag="LK889_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBK5095857.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            1897..2544
                     /locus_tag="LK889_001622"
     CDS             1897..2544
                     /locus_tag="LK889_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095858.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            2804..3148
                     /locus_tag="LK889_001623"
     CDS             2804..3148
                     /locus_tag="LK889_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5095859.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            3153..3434
                     /locus_tag="LK889_001624"
     CDS             3153..3434
                     /locus_tag="LK889_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095860.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3537..3851
                     /locus_tag="LK889_001625"
     CDS             3537..3851
                     /locus_tag="LK889_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK5095861.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3829..5523
                     /locus_tag="LK889_001626"
     CDS             3829..5523
                     /locus_tag="LK889_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK5095862.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            5543..6721
                     /locus_tag="LK889_001627"
     CDS             5543..6721
                     /locus_tag="LK889_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5095863.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            6684..7370
                     /locus_tag="LK889_001628"
     CDS             6684..7370
                     /locus_tag="LK889_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK5095864.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            7370..8530
                     /locus_tag="LK889_001629"
     CDS             7370..8530
                     /locus_tag="LK889_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK5095865.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            8540..9415
                     /locus_tag="LK889_001630"
     CDS             8540..9415
                     /locus_tag="LK889_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095866.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            9412..9723
                     /locus_tag="LK889_001631"
     CDS             9412..9723
                     /locus_tag="LK889_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095867.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            9713..10066
                     /locus_tag="LK889_001632"
     CDS             9713..10066
                     /locus_tag="LK889_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK5095868.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            10056..10457
                     /locus_tag="LK889_001633"
     CDS             10056..10457
                     /locus_tag="LK889_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095869.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            10450..10854
                     /locus_tag="LK889_001634"
     CDS             10450..10854
                     /locus_tag="LK889_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095870.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10866..11471
                     /locus_tag="LK889_001635"
     CDS             10866..11471
                     /locus_tag="LK889_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5095871.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            11491..11853
                     /locus_tag="LK889_001636"
     CDS             11491..11853
                     /locus_tag="LK889_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095872.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11856..12038
                     /locus_tag="LK889_001637"
     CDS             11856..12038
                     /locus_tag="LK889_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095873.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            12055..15474
                     /locus_tag="LK889_001638"
     CDS             12055..15474
                     /locus_tag="LK889_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK5095874.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            15525..16262
                     /locus_tag="LK889_001639"
     CDS             15525..16262
                     /locus_tag="LK889_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5095875.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            16272..18563
                     /locus_tag="LK889_001640"
     CDS             16272..18563
                     /locus_tag="LK889_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5095876.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            18587..20677
                     /locus_tag="LK889_001641"
     CDS             18587..20677
                     /locus_tag="LK889_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095877.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            20694..20843
                     /locus_tag="LK889_001642"
     CDS             20694..20843
                     /locus_tag="LK889_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095878.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20840..21286
                     /locus_tag="LK889_001643"
     CDS             20840..21286
                     /locus_tag="LK889_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095879.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21288..21425
                     /locus_tag="LK889_001644"
     CDS             21288..21425
                     /locus_tag="LK889_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK5095880.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            21463..21756
                     /locus_tag="LK889_001645"
     CDS             21463..21756
                     /locus_tag="LK889_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095881.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            21753..21977
                     /locus_tag="LK889_001646"
     CDS             21753..21977
                     /locus_tag="LK889_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK5095882.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            21974..22999
                     /locus_tag="LK889_001647"
     CDS             21974..22999
                     /locus_tag="LK889_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5095883.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            23189..23262
                     /locus_tag="LK889_001648"
     tRNA            23189..23262
                     /locus_tag="LK889_001648"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23223..23225,aa:Ile,seq:cat)
     gene            23939..>24170
                     /locus_tag="LK889_001649"
     CDS             23939..>24170
                     /locus_tag="LK889_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5095884.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQL"
BASE COUNT         8581 a         3635 c         4974 g         6980 t
ORIGIN      
        1 agaaaaagac agaatgaagg cagtagcttt ggttgaattt gctgaggcgt ataaatcagg
       61 agaactcatt tcacgtacaa aataaaaaaa gtcggattcc tccgactatg aataatattt
      121 ccgacataag tattatatca taattggggg aatcagagga tggtactttt cgatgtaaag
      181 aaatatgaga caccaagcgc gaaagatgtt gatatggaac gcacaaaaca taatgtcgct
      241 gtgtttcttt cagcatatct atcagctaga tgtagagtag ggcaacctcg agaaccaaaa
      301 gtgacagcat cttattcctt ggttccacct tctacagctg atcatatatt tgaagcagaa
      361 agaatgctga tcgataaaga agaagcacag gaagaatttg agtatctgca taaattgttt
      421 gttcgaggct attcagcgat acagcatccg cataagcctg atgtgactga aaggcgcaag
      481 aagatattct atgatcgtta tatcaatggt ctgcccattt atgtaactgc tcaaaggaat
      541 aatactagcg aagaatcggt taaagtagaa tcaaacagaa ttatcatcca atttgcttca
      601 tcgttagaac tggttgcttt caagtagcca gtttttacac tttttatacc cttttattac
      661 caatttggtt tccattttat acctttttta taccaatcac ttacctattc aacgttgtat
      721 tatgatagtg tcgaaagatt aggaaacagg atcgacaaaa taaaatgtaa gggaggaaat
      781 ctccctcatc gtttaattaa gcttcgatag gcagcagcgg aaatattaag aataaggatg
      841 tgaatttcaa ctccttctaa attgttctta ttatctatca tccgttgctg tctattaatt
      901 tatgtattgg agggtatata aaatgaagat cgcttccgaa aaaattgatc aactaacaat
      961 tttagtaaac caaagaatcg aagatattat caaagaattc gaaggtgaaa agattaagaa
     1021 cataaagctt actttaatgg atcataagtt cgaattaaat tcgacaaaaa ggaagatcgt
     1081 tgagcttata gaagaagaaa tagctctcca gatcaatgag caacaattag ctaattaatt
     1141 tatgtattgg aggggaaatg aatggaagtc gaacaaataa agtatcaagg tatggatcgg
     1201 gaacggttga ttgatattat tgagcaacaa cgtttagaat tgatccaaaa agaagctgta
     1261 tgtaaagagt acaaaaaaca tttggaacaa gtaattgagt atcattcagt agaaaagtac
     1321 agatcagtgg tgcaaaaaaa tagagcaacg agtcctgatt ctcgtcaaga tgtatcgatt
     1381 agtatcaaga cgccagtatt taattctgtt aaatcagaaa agctagaaag tatctcaact
     1441 gctttggtag aaaaagcagc tgaaatgaac tccctagcaa atgaagttag tcgtcttatt
     1501 tagtcatatc gataacttga gagtctttgg tgattccgtt ttttatagaa ttaatggttt
     1561 gttcagcgga atatttagtc aagtatgttt cgctagttgc tactacttca ttgttattgg
     1621 acttaataac gaagtaatat tggccattag ttgcttctct tataacaaag tacaaatttt
     1681 tcaccaccta taatttattt cagcggacca ctcgctgata attaaaatta taagctatgt
     1741 ataaaatttc acaatatcaa tttgtcgctg tggcggaaag ggtagacgca ataaaggatg
     1801 aatgggaaaa gtcaatgagt aattatctga aatttacaag tcccctgttt agttatctaa
     1861 gattatttga taatattatt tctgaggtga tagaaaatgg tgggcgtagt taaatttctt
     1921 gggtttacat ttgatggata tggtacgtta ggcgaatggg taggtattat tttgatagtg
     1981 attacaattc aacaaacatc aaagtactac tggtattcta atagttctaa aattgtatca
     2041 agaataactg aaggaataac caaaaaagat attactgtcg tcggtaaata tgggtttact
     2101 ataactttat taaatgaagg gaaatctgaa accgcaataa agttttgcgg gattataggt
     2161 aaatataaca ttttccaacg ttttatcaga aagcattggt acaaatatat ttacaaatac
     2221 cctgtagtta aaaagcttat tgaaaaatat gtggaccacg aaatatctta tctcaatcca
     2281 acagacctta ttaatcaaga attccagaat attggatact tagaaaaagg tctaaaaata
     2341 ccaattgtaa atgaaagttt agttactgca atggtagcta acataaagaa cagcaagaaa
     2401 ttagaaaaga gatataaaaa atgtaaaaaa ttgaaatttt cctttttatt cttagaatat
     2461 aatggcaaag aatattatca agatatttat gttttgcgag gctcgaactt atataaagac
     2521 attaaaaata agatttcagt atgagatttt ttaaaattcg tagatcactc tttgagtggt
     2581 ctttttattt tgcgtaaagg aggcacgacg atgtatagac cacaatactt agaacagaag
     2641 catgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta tagaagacca
     2701 ataaagagcg atacatataa acggaaggaa agcaatgaag ttattccatt gtatggcaaa
     2761 agaatagcta agtattaaat aagattgcga aaggagatgg gacatgaccg aggaattcta
     2821 tagatggtta ttacagttga taagagaaga tcgtttggtt aagttctatc agtctcctaa
     2881 atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag
     2941 aagactaggt aagtatcata gagtagagaa cgttcatcat ataaaggaag tcaaggatag
     3001 acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga
     3061 agttcatggc agatatctta cagcgttaga taaacaagag aagaagatag aaagcttcgc
     3121 taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgacaat ggcagagagt
     3181 atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt aaacgtttga
     3241 tatacgttcg ctatgtaggt attagggatc tgttatcaga taactgttgt agtaaataca
     3301 aagtgaatca agtaagagaa gcgttgaata aagataataa cgtcgaaaga ataaaaaatg
     3361 tttttggata tagtattgaa gagattaatt attacattga cttcgctgaa gctttcattc
     3421 cgatggtgag ataacccccc cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg
     3481 agggggctgt caggaaaaga gattttttcg aactttatca tgaaaggagg gctaaaatgt
     3541 ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt acgccgctct ttaataagcc
     3601 aattggaaag tcagaaaaca aatattgagc cattcttaga taatgttgat cgttatatca
     3661 gtttatggga aacggcgata tcactggaag aagatatatc cgagaacggc attagactgg
     3721 agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat
     3781 tgatgttgga taaacttgcc attactcctg aattggtagg tgaagcaaat gaatcaattc
     3841 ctgagttata agcatattga aaattggttc aaagctatag aagaaggcac tatcaaggta
     3901 tgcaaagagc aattattgct aaaaaattat ctagaagaaa gagtctttac tagagaagat
     3961 atttacttcg ataagcagat ggtagaggat tcaatcaata taccagcaca atactttcca
     4021 ttcgaattaa ttccgtggga aaaatttcta caatgtttta tttatggtgt tcgatggaaa
     4081 aaagataaaa cactagtgtt caatagatat ctttcattaa tgggacgtgg taatggtaaa
     4141 actggttttg cttcttggaa caacttcttt ctactaaccg ctaaacacgg tattaaaaat
     4201 tatgatattg atatctatgc caataatgaa agccaagcaa agactagttt tgatgatgta
     4261 tttaaagtaa ttaaagatca tcctgattta gataaaaaag tatttaaagc tacgaaggaa
     4321 gttattcaaa atatcgctac aaacagcaaa cttcgttata acacggcaaa tgctagaaca
     4381 aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat
     4441 tcaatgataa atgtggctac ttctggtggt ggtaaaattc gagattatag agaattttat
     4501 gatacaacta atggtcatgt tcgtggtggt ccgcttgatg acattataga agaatcaaaa
     4561 atgattcttt ctggagaact tggaattgac aaggatggag cagaattttc tagtttgttt
     4621 ccatttattt gtcgcttgga taacgataat gaagttgatg atcccgacat gtgggaaaaa
     4681 gcttgtccaa ctattaatta caatgcagat ttaaaacgga aaatgtttca agaatactct
     4741 caaatgcaac gtaatgctgg tttaagactt acgttcatga ccaaacgaat gaacagacct
     4801 atggaagata cacgatttgc tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa
     4861 gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg attttgctga tagacgagat
     4921 tttgccagcg ttgggttgct aggaaaatac gataaagatg tttattttac acaacatact
     4981 tttatccacg aatcagccct tcgattacaa aacatcaaac gagaggttat agatatttct
     5041 atagatcaag gaaaatcaca gatcgttcat ggaaaaaata tagaagctga ttatattgta
     5101 ggttggtttc ttgaaatgag taataaatat tatattaaaa aaatcgctat ggatatgtac
     5161 cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt ttactgtgga aattgttcga
     5221 agcggatctg ttacacatgg tatgttaaaa gatctggttg atgacctttt tattaatcaa
     5281 cgtttatttt ttggtgacga tgcgattatg cgttggtatt gcatgaatgt atatgaagag
     5341 catatttcta atggaaatat acgctatgaa aaaatagaac ctgaaactag aaaaacggat
     5401 ggcttttttt cattccttca tggtttgaat tttttagatg atatttatga ttctgctcct
     5461 gtaacagtca caaatagctc agtagaaaat acaggaactg gatttactcc tctagtattc
     5521 taacttgaaa ggaggtgaga aagtggggat ttttcaaaag gcggtaggat acttcacaaa
     5581 aaaagcaacg gttcctttag aagaatactt ttgtaaattg caagttgatt ttgtgtatcg
     5641 aaaatttgcg attgaaactt gtattgattt gattgcaaat gcgatgagta aagcggaatt
     5701 caagtcatat gaagatggaa aaaataaaaa gaatgatctt tactataggc tgaatgtagc
     5761 tcctaataag aaaaataatg caacagaatt tagaaaaaaa ctgatcagga gattaatatt
     5821 ctacaatgaa gtattgatcg tttctccgtc taataattct agcgaaatat ttattgcgga
     5881 tagttgggat gtcacagaat atgcattgaa agatgatgtg ttttctcaag tgcaaattaa
     5941 caacatagtc cttgatagag aatttctaga aagtgatgtt atctatataa aatacgcaga
     6001 tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg tatgggaaac tcatttctag
     6061 tgccatgaat gtttacaagc gctctaacgc tcgtagatac gtactgaaag ggaatttatt
     6121 ccgaccgcaa gacaatacaa cacaagatca aatcaataaa atgatgacat cacaatttaa
     6181 ggcttttatg gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt
     6241 agaagatttc agcggaaact tccaaagcaa ttcaagagat ataaaaaact taatagacga
     6301 catctttgag atgacagcag cagcgtttca cgttccgaaa aacctactaa agggagacat
     6361 gagtgggtta tcggatcaag tggacgcttt tttaatgttc gaaatcatac cgattgctga
     6421 acttattcag gatgcgttta acgctagtct ctatgaagta gaagaatact tgtcagggaa
     6481 ttttgtacgt gtggatacaa ctatgatcaa gattactagc ttcaaagatt tggttgacgc
     6541 tattgatgta ggcattagaa atggagtatt tacaatcaac gaaggaagag agcgcgttgg
     6601 aaatgatcgc tctgataagg cgatggcaga tgaaatattt ataactaaaa acaatcaaca
     6661 agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac atttctagca gtaaagaatg
     6721 aaggcgcagt accgcaaatt tttattcagg gatttattgg ttctagttgg ttctttgaag
     6781 ggaatactga caagggaatc aaaaatattt tggatagtct aggtgatcaa gaagaaattg
     6841 aagtagtaat taattcaaac ggtggagacg tatttcaagg gattgctatt gggaacttac
     6901 ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg cttagccgct agtgctgctt
     6961 caattatcgc aatggctggc gatactataa aaatctacaa caatgcacaa ttgatgattc
     7021 accgcgcttc cacatacgga gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac
     7081 tggaatcaat tgataaatcg gtaaaggctt catataaaac acgattcaat ggcacagatg
     7141 aagcattgca agaacttctt gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt
     7201 atggattggt cgatgaaatt atcgatgcag aaaatagctc aggtactgaa gctaaaaaag
     7261 aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg
     7321 catttacagc agcattaaat aaaacatttg gacaaggaga tgtaaaataa tgacagttaa
     7381 aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg aaagctttta aagatggtaa
     7441 cgaagaatct tttagcgcag ctatggtaag cttatctaag gaaattcagg ataaaatttt
     7501 agaagaagca acagcaaaaa atcaagatca attagtatta atgaaccgtg gtcagcgtgt
     7561 attaactaca caagaaacaa aattctataa cgaagtagtg aaaaacgaag gttttgcagg
     7621 ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta tttgaagatt tagaacaatc
     7681 tcatccacta ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag aatggattgt
     7741 gtcacgtgga gtcaatccag catggtgggg taaactttgc gaagctgtta aaaaagtttt
     7801 agataatggc tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt atattcctgt
     7861 atgtaaggca atgcttgatt taggtccagt atggttagat cgttatgtcc gtactgtttt
     7921 agtagaatca ttgagaattg cattagaaca agcaattgtt gatggtactg gtaaagatat
     7981 gccagtcgga atgatgcgtg acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa
     8041 agcagaacct attacagctt tagatgctgt aactatgggc ggtttgatgg cgcgactatc
     8101 aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt
     8161 ggtcctaatt gtgaatccaa ctgattactg gtctaaagta ttcccagcta agactgtact
     8221 aactgctaat ggagaatacg tacaagtatt gccagtacca gtttcagatt tgcagtcaac
     8281 ggctgtgcca gaaggaaaag cagttattgg ggtagcctca gattacttca tgggtgtagg
     8341 atctacacta aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta
     8401 tctagctaaa caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga
     8461 tattagcgct ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac
     8521 acaagcgtag gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa
     8581 atgggagctt gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt
     8641 gcttttcagc agacacgatg atcagattcc tatttttttt gagaaggaat atggtgttga
     8701 agttcatgtg tacgatgatc tgcgggacga caaagagtat attccttcga ttaaaccata
     8761 tttatggtgg aaatatttag aagaagatca ttcgcgtgag gacgaccgat atttctatat
     8821 cgattcggat gtcattttca ataaaagaat taatttgcgc aaattgcctt ctaaagatga
     8881 tgtttggtat tgtagcgact gctgtagtta tctaagtctt gattatatta gaagctgtga
     8941 aaacggagaa aatattctaa aagatatggc aaacattgta aatgttacag tagaatcttt
     9001 ggaaactata aacactaatt caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa
     9061 ttattggaaa aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa
     9121 aacaaatata caaatctgga cagccgagat gtgggcacag ctttggaaca tgatgtattt
     9181 caatattggt cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga
     9241 aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt
     9301 atttttcaaa gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa
     9361 caagaaaaaa tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct
     9421 gaacaagtga ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac
     9481 gaagaagatg agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa
     9541 gtgagtacaa cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga
     9601 tgtagatacg actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta
     9661 ttaggtttca ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa
     9721 taatcgtaga ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac
     9781 caaaagaaat gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa
     9841 atttagaaat ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa
     9901 attaactaag aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca
     9961 atattttatc gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt
    10021 ttatatctat ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa
    10081 aagaacgttt aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc
    10141 tcaaactctt tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca
    10201 ttctcgaaac aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc
    10261 tatatctaac tttctattca gaaaatagag aagatttaac aggagattca ctagatatta
    10321 tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat catttaaaac
    10381 tagagaacca agatcgctat atcgatcaat tggtatttac gtttagacga ttattgaaga
    10441 gtgattgtca tggctaaaaa tagttgggag ctaaaaataa atggacatga tgaacttctt
    10501 gtgcggatgg aacgctattc aagcgagagc gaacgactga ttaacgaagc attgaaatca
    10561 aaaggttcgg ctattgcagt ggataggatt acagaaaaaa ttcctgtttc tgaagcagat
    10621 ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca atacattaat
    10681 ttgggtttca tcattagacc tacaagaaaa tttgagtatt taaaatatcc tgatttgggg
    10741 ataggtactt ctaaaagaaa tcagccggac gaatttatga gaagaggatt aggtcttgca
    10801 cttgatccaa ttacagaact tctgattcgt caattcgata aattaaataa ataggaggaa
    10861 caacaatggc taaaacaaca acagtaacaa cttttgacaa tataagtatt aaaagaattt
    10921 cttttaattt taagaatgca acaaatgcaa tttcaactga ttgtaatgga caattagatg
    10981 gcgaaacaga aatgcagaga attgtaaaaa aatgcggttc aacagaagta aaatcgaaat
    11041 ctaaaccaat caatatgacg gtaacaatta ctgcacatgt accgatggaa gtttatcgac
    11101 gcttcaatgg gttgaaacaa gatgaacgta ttaaatcggg aatttactct tatggtcctg
    11161 attccgtagg ggaagatttc tctcttgctg cagagatcgt ggatgatttc gaagaaaaaa
    11221 ataagctgat tggtatgtta gcatgcactt cgaatacagg attaacattc tctattgaaa
    11281 atggtgcgga tgaagtagct gcgttagaac tagaaacaaa agttatgcaa gatgaatttg
    11341 gtaaatttta tcatgaagca attgttgcag aacttgaaga agacttaaca gatcaatgga
    11401 tgacgaatct atctgctgat gtgattaaaa aaacttcagt tgtgacaact acggccactc
    11461 aatcacagta aaaaaaacgg aggtagcgaa atgaacgaag attactcaaa aattgaacta
    11521 aacgatggaa caattttgaa tttagaacct aaactgaata tcaagaaatt attgatgatc
    11581 aatagagatt ttaacacaga cgagtttgca aaaatgactg tgggaaaagg atccatggat
    11641 atttctgtta ttcaaggtgc aaaggctgtg tatattgctt accgccaagc gaacatgact
    11701 gattatattt cattcgatga atttatcgat aaatgggatt ttgatatggc tactgccagc
    11761 tatatttatc aattgatgat gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa
    11821 aaagcaaata aggaaaaaaa gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt
    11881 gggtcgatgt ctattcgatg ttgaccgacg ttttttctat gccttcagat ttggttttaa
    11941 gcgatatctg tttagatgac attttgcaaa tggcttacaa caagagtgct tatgaaggat
    12001 ggaaaaacta tgcaataaac caatcccaga aaaactaaag aaaggaggta aaaaatggct
    12061 aaaaagagaa cagaagcaga agtaacattc atagctaacg atgacggatt gaaatctacg
    12121 ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact agaacaagct
    12181 caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc ttctttagaa
    12241 aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gccaacgttt agccaatgcc
    12301 aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgagagaga actgattaac
    12361 caacaaacag cgcaacaacg tttgtcaaac gaaattgata aaacgagtaa tgcactagct
    12421 caagcaaaag gcgaaataca gacgtacgag tctacaatgc aacagttgga tagtgaacaa
    12481 aaaaatgttc aagctagtgc ttctctgatt gaatccgaat acaaaaaatg gcaagcaact
    12541 gctggtcaat cagcttctga atccgagaaa ttagcgaaag cccaagaata tgtttctcaa
    12601 caatctgaaa atgcagagaa aacgatagat atcctgagac gacagttaga agctacacag
    12661 tctgagtttg gcgctacatc cacagaagca atgcagatgg aggcgaagct taatgatgct
    12721 gaacgtgaat ttgaagagtt aggacaagct gctaaaaatg tagatacaac taacttggac
    12781 gatatcggaa gcaaaataga tatgaataat ctaatggaag cttctgacgt tttaagcgac
    12841 attggcgata agcttacaga attagggaaa caagcagtgg actctgctaa tagtgtaggt
    12901 agttcccaga gtaaaataca agctaatttt ggtttgacta aacaagaggc tgaagaatta
    12961 acgaatgtag ccagagacat ttattataaa ggttttggag aatcgttaga tcagtccaca
    13021 gatgcattga ttttggtaaa gcgtaattta ggcgatttaa ataatcaaga tttacaaaat
    13081 atcacggaac aagctatggt cctagaaaac accatgggcg ctgatatgga tgaaacgtta
    13141 cgtggtgtaa atggcttaat ggtcaatttc ggcttgagcg ctcaagatgc aatggattta
    13201 atggtttcgg gtactcaaaa cggtttagat aaaacgcacg aattaggcga caatatggca
    13261 gaatatagcc aattatggag tcaaatggga tattcagctg atgaaacgtt cggaatgctt
    13321 caaaatggtt tagatgcggg tgcttataac cttgataaag tcaatgactt agttaaggaa
    13381 atgggaatat cgttaacaga tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt
    13441 actagaaaag cttttgaaga gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat
    13501 tccatgattc aagattttag caatatggat ggtcaatacg accaattaaa taaagcttct
    13561 acaatttggt ctgcacttgg cgaagataac gcgatgaaag ttgtccaatc tttgactgat
    13621 gttaaccata catttgatga tgttagtgga tctgcacaaa aaatgaatga agattctact
    13681 actccgttac aagaattaaa cggaaaaata gctgaattaa aggattcatt agctcctata
    13741 ggcaacacaa tcatagatgc actcgaacca gtaattgatt ttctaggaaa gatggctgat
    13801 gcgtttaata atcttccaca accagtacag gattatgccg tagcgattgg cggattgact
    13861 gctgcattta ctttattaat gccaataata gttggcttca tggctctagg tggtcctact
    13921 acattaataa taggagcagt tattactgtt attgctggag ttatagcaat tataaaaaac
    13981 tggggcgcaa ttactgactg gtttaaggga atatggagta aattcactga ttggttgggt
    14041 ggtacttggg aaagtataaa agaaggtgcc tcatcagttt gggatggagt taaagaaacc
    14101 tggtctggat ttgtagattg ggttcaagat atttggcaag gagtttctga ttggtttgga
    14161 gagctatgga gcggattagt tgaaggagct tccaacatct ggcaaggagt ccaagagact
    14221 tggcaagcat tcgttgattg ggtttcaaat atttggaacg gagtcaaaga agtatggtcg
    14281 attatttggg cagacattgt aggaattgtt caaataccat ggaccttaat aacgtcattg
    14341 attcaagccg gtattaatat tatcgtgggt atttttgatg tagctggaca gttattaggc
    14401 gcagcttggc aagctgtttg gacacctatt tctgatttcc ttaaaaacac ttgggatact
    14461 atgacacaat gggtaagcat cgcttggaat ggaattgtaa ctacattcca tactatattt
    14521 gatccagtag tggcatggtg gaatggtata tggacagcta ttagtactac ggcttcaaat
    14581 atttggaatt caattagtgc aacagcttct agtatttgga acagtatcaa gaatacaatc
    14641 actagcttgg tacaagcagc tgctacagta attcaaaata tttggtcaac tgtatctagt
    14701 tggttaggtg gaatttggaa ttcaatcagc tctacagcat caaatatctg gaatagcgtg
    14761 actagtagta taagcaatgc tataaacgca gctaaaagtg ccattcaaag tgtttggaat
    14821 agtatatctt cgtggatcag cggaatttgg aacggtatca aaaacactgc tttgaatctt
    14881 tggaatggaa ttacaagcac tattagctct aaagtaaacg atggaaaaaa tgcaatttca
    14941 agcggttggt ccaatctaac aggtattgtt tccgacatat tcaataatgt taaaagtaca
    15001 attgctaaca tttgggaagg tatcaaaaag actgttagcg ctccaattga ttggatcaga
    15061 gataaaatca gcagtatctt tgataatttg aatatttcta taccacatat tccgttacca
    15121 cattttaaat tgagcggaga attcaatcca ttgaagggga aaatcccaac gttgggtgtt
    15181 gattggtatg cgaaaggtag tgtgtttaat tctccgaata ttatcggtgt cggcgaagca
    15241 ggacctgaag cagttttacc tttgaaaaga tctgtgctgc aagaaattgg tgatcgtatc
    15301 ttgagtagca catcagtttc atctagggca caaacgattc aacctgtgaa taactacgaa
    15361 ttcaatttca caattgatgg taacgcagat gaggttacta tgaagcaaac aactcaacaa
    15421 atcattgata gcattacaaa agttcaaaat gataatgctt cggcatggcg ttaaacagga
    15481 gagtatttct cctgtttttt tagtattaaa aaggatgtga aaaaatgact gattgtatac
    15541 attctataat cgatggattt cctgattatt tgcataaatt ggctttagct gaaagaccaa
    15601 ccataccttc tccaaaaaga cagagagttg aaacttctgt tttaggaagg ttaggtggct
    15661 tagtacaaga ttactcgttt gaagacatgt cgtttacatt gcactataac tatttagagg
    15721 atgtggaaga ccatcaagcg ttcaagcaat cgttttatat catgcgtcat tggttaaatt
    15781 atgcaaagaa attagaattc tctgatgatc ccaacgtcta ttatgttatc cagactatcg
    15841 atattgggga tgcagaaaac gatattgttg aatggggaga gttcgatgta aatattactg
    15901 cgaaaccatt cgcaagagtt caagaagatg tacctataac cgtagataaa ccacagtcat
    15961 ttaacttgct gaataatagt ttagaagaaa gttttccaaa gattatcatc actccttcag
    16021 ctacttcatg ccagttcatc ttaaatgatt atgtgtttag ttttgaaggc ttagtagtag
    16081 gaactgacgt agtcattgat agtgatttga tgctttgcta cgaagagcaa tcggacggag
    16141 atattttaga tcggtccaac aaaatgaaga ccatgcaata tccgacattg caagtggata
    16201 ttaattattt taattgtact ggtttgagca aaatacaaat ttatcgtaat gggttaaggt
    16261 aggtgaaata gatgatcgat aatttaataa ctatttacga taaaaacgac gcgaataatt
    16321 tagctgaaca tttatatgat acgcaaggtt taggcgcttt gtcagactgg ttaacagcta
    16381 ctgttagcaa taaattaaac ggagccgaga tatttcaggg tacttatcca ataagcggaa
    16441 ctaatgcaga tttgattgta gaaggacgta ttattcagtg ttatgtagat gaaaatcgag
    16501 caaaacagcg tctacggatt tattatgcaa agacttctgt aataggcaat acgatagaag
    16561 taaaagctga acctattttc aatgatataa gaaaatcggt gttgaataaa tatgacagtg
    16621 gaacagaaaa gatcactgct agtcaggcat ggcaaaacgc aaaaacttta gcgaaaccag
    16681 ttattccttc acagttttct ttctcgtcat tagtagatac gcttgctaat gtgaagatag
    16741 aaaaggcgaa ttttttagaa ttctttggtg gaaaagaggg atctattcta gatcgatttc
    16801 atggggaatt tctaaaagat aataacacat tacgtcatga aaaaaggcta ggcacggatc
    16861 ataaaatcaa agcgatttat actaaaaact taactggtct tgacttagag attgatgctc
    16921 aaagtgtttt agttggagtt tatccattca ttagcagctc ttcagaagga gaagacgaga
    16981 tcactctacc agaagaagtt attttcacgg attacgtgga tgattatcct gctggatatg
    17041 tttcttttgt tgattttaaa gacaaagcga ctgatgtagc cacattaagg gaagctgcta
    17101 aagactggtt gaaaacaaac atagataaac aaaaaccaca agtgagtggt tcgattgaat
    17161 tagtaccatt gaggcatcaa agaggctatg aaaaatttgt tgatctagaa aaagtttcga
    17221 tgggtgacgg agtagatgtg tatcatccac agttaaaagt gaatatgtca gcgagaattg
    17281 tggaatatac gtttaatgtt ctaaccaatt catacgataa attagttgta ggaaacgtca
    17341 aaacaaactt cttagaaaac acagagaata atgtcagcaa tttgattaat gatgccattg
    17401 atcaattgaa aaacggtggc gaaatcagtg atttactcaa tgatattgta gatcatcaaa
    17461 ctgatatgat tactggtcaa aatggtggtt atgttttatt agatcctaaa gaagcgccta
    17521 gtcgtatttt gattatggac acaccagata agaataccgc aaggaatgtt ttacaaatca
    17581 acaatgctgg tattggtttt tctaaaactg gcattaatgg aacatatgaa acggcatgga
    17641 cgttagatgg cggattcaat gcctcgttta ttacggctgg tgaaatagta ggaattacta
    17701 ttagaggtac tacattaatt agtgatggtg ctgattatag aacaagtatt gctaatggca
    17761 aaatgacttg gtactcaaaa aaagttaaca aagatattat ggagctagaa gcacgtgatt
    17821 atgtaagtgc tgatgccggt attgtatcat acaccatgaa aactggtggt ggtttcatga
    17881 ttagaaatcc acagggtaac ttggttttta gtacgtggga taatggtaat aacagaccgt
    17941 ttctatcttt tggtgcgccc aatttcaggt atagcaatgc tagttatgta acttctggcg
    18001 acggtagttc tttaagcatt aatggtagtg cgggtaactc atgggaattt aaggtagctg
    18061 gtaggactat gaaatttact agtgatggta tgctaacgtt accaggttgt ttttttggtt
    18121 catgggaaga tgggaaactt gctaggtttg aacaatcaac ggtacaagta tataaagatt
    18181 ttactgttag aggtactaaa aactcaactg taccaacaga acattatgga caacgactat
    18241 tgaacgctta tgaaactcca gaatattatt tcgctgatta tggggaagcc gttacaggtg
    18301 acgatggtaa agttcgtgtt gatattgacc ccatgtttgc tgaaacagta aatctaagtc
    18361 ggtatatgac acatgtgaca cctacagaac tagttttgtg tgctgttact catgaagata
    18421 ttgaccattt catcattgaa actagtaagc caaacgtatt agttagatgg aatttagtgg
    18481 cacaccgtct agggtatgaa gatattagat taaaagagga tacagcatat gatagcacag
    18541 tgcttgacca aaaacgtttt taaaacgaag acaaggaggt atataaatgg ctagcagttt
    18601 atataatttg gctttagatt tcagcaaaga attaaactac accaaagcta ttatggctcg
    18661 tcaaggtgat aaagggatta cggtgacggt taaaccgttt ctaaatggct tgcagatgga
    18721 tacgagtggc ggaacattta ctttaaaagg aacaacacca tctaaccgtt acgtagataa
    18781 tgttgcaact agtgtaacta gtgaagaagt cacgttttct cttgatggca catttatgag
    18841 tgaagcagga tattataaac actgctacgt agaatataga aaagacaatc aaattttaac
    18901 aacgcaagat atcatttttt tctcactagg agtgtctgac atttcgcaag gccaagccga
    18961 tgaatatgtt tcgcaattag aagagttgat tcgaaagtac aacgaaactt ttgatgcttt
    19021 tatggctgaa atcaaaggta gagtggatag cttaaatcaa cagattactg atttaactgg
    19081 tcaagctaaa acgctacaag acaagttaga tgctctgaaa gaagaaattt ctaagttagg
    19141 taacttacaa gtgatgtaca gtaacagcat cgacttcggg ggatatgatt atagcggtaa
    19201 gccgaattta atgtccaaac taaaatcgag cgattttaac gttggttacc acgggtcact
    19261 aactttggat aacgaaaagt tacattttac ttctgatggt acaggaagca ttattatgtt
    19321 tacgcgtatt aatacacctc agcttactag tgggaaaacc tatactctga gtgcgaaagt
    19381 tcgatttgat gaaggaacta caggagctat tgataaatta cgtttggtgt atcgtacatc
    19441 accaggagaa aagatattat tggaagcaaa tagtacaaat attacaacag atgatgtagg
    19501 gaaagaaata acaatcaaag gtacagctaa cgttaattat caaatcacaa atttagatcg
    19561 attttatatg agtattagct ttgttgacag ggataaaata aatggcggat ttaagttgta
    19621 cgacatcaaa atcgaagaag gctcaacagc cacaccgtac cagcctaatc tacttgatgc
    19681 accgtattat ttgagtaagg tggctttggg tgagaatcta attaaaccag aatcacaaca
    19741 accagttact aatagtaact atcttattaa cacctataac attaaaccaa tggtaaaagg
    19801 taagaagtat accatcacac ttgaaggaac taagccagca acacaggttt ttagaccatt
    19861 tttcacacga gcaacaggag atgcatggga ggttggtgac ttacaaccag tagaaggctt
    19921 aactaatgtg tggtctaaaa catttacagc agcagatgac tcacacccta ctaccccaca
    19981 agtgcagatt tatcaagtac caagcacaag tgtaggacaa tgtacaatta agtggttaaa
    20041 actagaggaa ggcaatactc gaaccccgaa tattagtgag tataaatatc gtggtactgg
    20101 tatgcgtgat tcaaacaatc caaaagatta tgtttgggat ctagcaccag aatatgtcga
    20161 agataatttg gccacagata ttaaaatttc tgaaattact ggtaaagcaa acaattatac
    20221 cgatgggaaa gtatcggaga ttaattcgca gttgactgct tcaattaatg aagtagacac
    20281 cacagctaag gatgctcaaa caaaagcgaa tgctaatgcg actgctatag atgaattaga
    20341 caataagatc gatgaacgca ttaatgatac agctactacc acattaacag ttacaaacgg
    20401 gaataccgga tcagcaaagc tttatcgtga aggaaaaaca gtttctatat attttgtggc
    20461 tttaaacgga aaacgcagtg gtggaaatga ttcaacgata ctaacaattc cagaaggcta
    20521 tcggccacca attagttttg agcaactggt tggctcgata gaccgttcta ctttgaacag
    20581 tgctcagtta tctattggtg cagatggagc cattaaatgg cgaagaaact caagttatgg
    20641 atcggattat acctttgcaa ttacttacac gatttagaaa agcgtgaatc gatatgaagg
    20701 cagcatatag accaattgaa ccttacggat tcgagcaaat cattgtgaat gatgaagaac
    20761 atttaccgga agaatgcaca gaagtcgaac caccgattcc aaattggaaa ccgaaattca
    20821 attactggga gggaaataaa tgaaaaacat ttggaaatat ggacgtactg gcggagagta
    20881 cgcaggaaaa gtattggacg acatgcttgt atccgttcct tacacagatc agccaccgct
    20941 tgaaggaatt cgtgctgatg gcgaaccgtt aacaatcgct gatcaaatgt ttgatcctaa
    21001 attgaatcaa tggattattt tagcgaacgc actagatcac aacgatttaa acaatctcaa
    21061 agcaatgtat gagtcgttag aaaatgagaa cggcgattta aaacagatca atgccaaact
    21121 catgctaagc gatgtagcaa ttaaacagga aaatactgca ttgaaagaaa aagcggatag
    21181 tttagcacaa atcaattcaa aaatgatgct tgcttcgtta caaaatagca aagacatttc
    21241 agaaattaaa gagcaactaa atccagcttc aaagggaggt gagtagtatg tttagtttta
    21301 gcgatgtgaa aatgatgtat gattggggct gtttcactaa cgaacaagta atggttttcg
    21361 ttccgttgtg cattactgaa gaaaaagcag ataaaatcat tagcaaagaa gagagcgcat
    21421 cttaattgat gtgcttttta ttttgattca aggagttgtc acatgattaa tttaggggaa
    21481 tggggagcga tagcaggatc aataaccgct atcgtttctt tgattttatt agtaataaaa
    21541 ccaattactg catctttctc gaagattact gagactcttt caaaagtaag tcacaattta
    21601 gatttgctga ctaaagattt agaatcgagc aaatcagatc gattgatgat tcatgaagaa
    21661 ctaaagaaac acgatgaaag attagataca catgcagaaa aattggtaga acacacgcaa
    21721 caaattaaaa ctttatttag ggagagaaga aaatgaataa taaaacgttc gaagtactaa
    21781 aatggttcgc actggtaatt attcccgcac tagctacttt cgtggggtta gttggtaaag
    21841 cgctcaattg gcagtacaca gatatctgtg ttgtcatcat tactggtttt ggcgcgtttt
    21901 tagggagtgt gttgggtgta tcaaatcgaa cctacaaaat gttctcggct gaaagcgaag
    21961 aaggaggaaa caaatgaaaa agaaaattac tattactgcg atgagcctgt taacggctct
    22021 ttttttatta cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc
    22081 gaatcaagga gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat
    22141 agatgcacca gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc
    22201 ttatactaca gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata
    22261 tgtttcgtgg ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg
    22321 cacatatgat aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga
    22381 tagtgcaaaa aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa
    22441 aggaatcatt tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata
    22501 tggttactta tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg
    22561 atttaccgat gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc
    22621 tggtgcagct aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg
    22681 ggaaatcgaa aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga
    22741 gtatattttc attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg
    22801 aatttataga ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt
    22861 atctttcaat atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa
    22921 tgatccagag ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt
    22981 aaatattcct aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac
    23041 aactataaaa attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata
    23101 aatagtcaca ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat
    23161 tgtttatggc tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg
    23221 ctcataaccg tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa
    23281 tcgttctgtg ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc
    23341 cactcaaatg agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca
    23401 tagttatact tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg
    23461 ggttaaattg tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca
    23521 taagtgtgtt acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt
    23581 ttgaaaaaaa gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt
    23641 ttgattttat agcactgata ctataaaata tagatatcat catattacac aatcttaata
    23701 ctaacttaaa aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt
    23761 tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat actttatata
    23821 gtcataacgc cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta
    23881 gactagacag aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat
    23941 gtcaagacgt caacgaagaa cctattcaaa agaattcaaa caacaaatcg tcgatctcta
    24001 tctcgctggt aagcctcgcg cagaaattat tcgagagtat gagcttacgc cttcttcttt
    24061 cgataaatgg atgaagcaag cacaatcaac gggctcattc aaagaaagag acaacttaac
    24121 accagaacaa gcagaattga tcgcactaag aaagaaaaat aagcaactcg
//
LOCUS       DAFBQW010000031        23803 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFBQW010000031 DAFBQW010000000
VERSION     DAFBQW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.032"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1250)
                     /locus_tag="LK889_001650"
     CDS             complement(<1..1250)
                     /locus_tag="LK889_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HBK5095885.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYL"
     gene            1523..3994
                     /locus_tag="LK889_001651"
     CDS             1523..3994
                     /locus_tag="LK889_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5095886.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4097..5833)
                     /locus_tag="LK889_001652"
     CDS             complement(4097..5833)
                     /locus_tag="LK889_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBK5095887.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5830..6534)
                     /locus_tag="LK889_001653"
     CDS             complement(5830..6534)
                     /locus_tag="LK889_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5095888.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDLHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6665..7168
                     /locus_tag="LK889_001654"
     CDS             6665..7168
                     /locus_tag="LK889_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBK5095889.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7128..7466
                     /locus_tag="LK889_001655"
     CDS             7128..7466
                     /locus_tag="LK889_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBK5095890.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7487..8905
                     /gene="ffh"
                     /locus_tag="LK889_001656"
     CDS             7487..8905
                     /gene="ffh"
                     /locus_tag="LK889_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK5095891.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9017..9595
                     /locus_tag="LK889_001657"
     CDS             9017..9595
                     /locus_tag="LK889_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK5095892.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9599..10120
                     /locus_tag="LK889_001658"
     CDS             9599..10120
                     /locus_tag="LK889_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5095893.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10199..10753)
                     /locus_tag="LK889_001659"
     CDS             complement(10199..10753)
                     /locus_tag="LK889_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK5095894.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11006..11281
                     /gene="rpsP"
                     /locus_tag="LK889_001660"
     CDS             11006..11281
                     /gene="rpsP"
                     /locus_tag="LK889_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK5095895.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11293..11544
                     /locus_tag="LK889_001661"
     CDS             11293..11544
                     /locus_tag="LK889_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBK5095896.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11669..12460
                     /locus_tag="LK889_001662"
     CDS             11669..12460
                     /locus_tag="LK889_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095897.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12630..13151
                     /gene="rimM"
                     /locus_tag="LK889_001663"
     CDS             12630..13151
                     /gene="rimM"
                     /locus_tag="LK889_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK5095898.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13141..13902
                     /gene="trmD"
                     /locus_tag="LK889_001664"
     CDS             13141..13902
                     /gene="trmD"
                     /locus_tag="LK889_001664"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK5095899.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14038..14385
                     /gene="rplS"
                     /locus_tag="LK889_001665"
     CDS             14038..14385
                     /gene="rplS"
                     /locus_tag="LK889_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK5095900.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14758..15420
                     /locus_tag="LK889_001666"
     CDS             14758..15420
                     /locus_tag="LK889_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095901.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            15499..18726
                     /gene="fss3"
                     /locus_tag="LK889_001667"
     CDS             15499..18726
                     /gene="fss3"
                     /locus_tag="LK889_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBK5095902.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18840..19154
                     /locus_tag="LK889_001668"
     CDS             18840..19154
                     /locus_tag="LK889_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5095903.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            19167..19541
                     /locus_tag="LK889_001669"
     CDS             19167..19541
                     /locus_tag="LK889_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5095904.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            19551..19895
                     /locus_tag="LK889_001670"
     CDS             19551..19895
                     /locus_tag="LK889_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBK5095905.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19966..21111
                     /locus_tag="LK889_001671"
     CDS             19966..21111
                     /locus_tag="LK889_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK5095906.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            21826..23685
                     /gene="ltrA"
                     /locus_tag="LK889_001672"
     CDS             21826..23685
                     /gene="ltrA"
                     /locus_tag="LK889_001672"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5095907.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         8092 a         4210 c         4937 g         6564 t
ORIGIN      
        1 tgtaaataga tccgtgcccg aaagttataa aagttgcgat aaccaaacgc aattcgttta
       61 atcagcttga tcttgttatt cagcccctca gtgacgccgt ttgagtggga caccttaaag
      121 gcatttgaaa tacccgattg gtagcgatgg aagatcgtga atttcttttt aaacgactga
      181 ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtacc tcgtttaacg
      241 aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc gttgtagctt
      301 aacagctcat caacgatttc tgtttgacta attggtcgtt taaacagtcg atgataatgc
      361 cgtttgagtg gctccaatgt cgtggaatct ttgagtaaga gtttccaata tcgcttgagc
      421 cgacggtact tcttctgatt ttcagagtgt gaggttcgaa aacgattcat gatctggaca
      481 cgtaattgat tgaagctgcg attgatgtgt tggacaatat gaaagcggtc agttacgatc
      541 tccgcatttg gaaagactgt tttaagcaac tgacaatagc tggcattcat gtccatcact
      601 aggtacttga cctttaaacg ggcctttcta ggaaagcgca ggaagtattg agttagtttg
      661 taaagtctgc gatcttcaag gatcgtaaat agctgattgg ttgtcccatc aacacaaata
      721 aaactcatgg aaccagaaac agaattcatt gatttgaact catccataca caacaccgtc
      781 ggaaggaagt tcatacgtgg ctggtaatgc ttcgttgctt cacgcaagat ccgtgtcacc
      841 gtcccatccg agacgaagta cttcttagcg attagtttac gtgattgatt ttcgtacagt
      901 tccatgagaa tcacctgctt cagttccttt gttagttgat gatgttcatc aactaagtcc
      961 gttttcgcag aaaaggtcct tccacaatca tggcagagaa aacgggtacg ttttaattct
     1021 aaaatagtta acttttcttt gactttcaac atctgagtgc gtgtcgtata ggaaccgttg
     1081 cggataatac gaaccgatcg acagtgttca caataggaag aagagtcatc caattttcct
     1141 ttaataaact gcgcttgaac gcctttatat tctttttcaa tcagccattc ttcatcaaaa
     1201 aataaatgtt tatctgttaa tccgagtaat tttctagtat gattatccat tgagagactc
     1261 ctttggtagg atgacgtttt ggtcgactaa attttaccaa aagtgagtct ctttttgagt
     1321 tgaaacgaag aaaaagtgtt agtagaaaat cgccttcgat tttccaccaa cacaaaatat
     1381 tatagaaccc tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg
     1441 aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa
     1501 cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa
     1561 agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt
     1621 aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc
     1681 cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat
     1741 ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt
     1801 accagttatc atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat
     1861 cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga
     1921 acaaacaata gatatagaag accttgtaat agatgacatt gttattttag agactggata
     1981 tcaaattcca gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt
     2041 gacaggcgaa gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt
     2101 cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc
     2161 ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc
     2221 tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt
     2281 ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc
     2341 agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt
     2401 aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac
     2461 attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat
     2521 gtctgttcaa aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt
     2581 ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc
     2641 tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc
     2701 ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc
     2761 cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga
     2821 aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt
     2881 gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc
     2941 aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga
     3001 taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta
     3061 cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt
     3121 ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa
     3181 tacagtagct atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg
     3241 cagtatagcg atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact
     3301 tgattctgac ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa
     3361 tattgagcga tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact
     3421 gttttcagtg atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag
     3481 tttgtttact atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat
     3541 cagagggaaa ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat
     3601 gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt
     3661 ttctacagca tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg
     3721 ttcaccgctg aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt
     3781 ttcggtcatc tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt
     3841 gctgttgagc attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt
     3901 agaatatttg tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa
     3961 aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa
     4021 aaagaacaac cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac
     4081 gagcagttgc tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa
     4141 gatgactgtc tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta
     4201 accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct
     4261 caaagatccg ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg
     4321 atagatcatc tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga
     4381 cagcattttc aattagattt ttgataattg gataaaggat atctgtttta gtagtaaatg
     4441 gttcgttctt tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat
     4501 tttgaacgat ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct
     4561 cgccatcttt tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt
     4621 tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta
     4681 aagtctcggt aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc
     4741 caacgaaatc cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc
     4801 ctagaatctg tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct
     4861 gccgaccttc tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat
     4921 gaatcagctg aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat
     4981 agcttaccac gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag
     5041 catcatcaat caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt
     5101 ctgtggtagt acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct
     5161 gatacaattc ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt
     5221 gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca
     5281 gataatagat cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac
     5341 ggcgaaaact ctcagttctc ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg
     5401 cttctaactg gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag
     5461 tagtactttt gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg
     5521 gacgggagtt tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag
     5581 tagcatccat taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt
     5641 tgttttgcgc tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt
     5701 ttgtcaggta gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt
     5761 gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt
     5821 ttttccattt catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa
     5881 gtactttggg tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtaaatc
     5941 tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg
     6001 gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt
     6061 tagttcaatc agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg
     6121 tccaatcgat agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg
     6181 tctgaatatt gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata
     6241 gtcgtcagca cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag
     6301 catcaaaatc ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc
     6361 catcgttgga agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc
     6421 taatccttcg cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa
     6481 cgtcaagagc gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat
     6541 ctcctttaaa taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat
     6601 tttacaaaag aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca
     6661 gtcaatgatt ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa
     6721 aaaagaagta ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc
     6781 agaagtgatc ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg
     6841 attcgatgtg tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga
     6901 agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct
     6961 agtcatcatg aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt
     7021 ttccggaata aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat
     7081 gactgtgctc tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa
     7141 agaccaatcg aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa
     7201 tgaactacat ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat
     7261 acgaagtaag ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg
     7321 attatgaaaa aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa
     7381 atatgactgc atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac
     7441 agcaaattca agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag
     7501 cttaactgaa cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga
     7561 agctgacgta aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa
     7621 cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt
     7681 gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat
     7741 cacacttggt tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat
     7801 gatggctggt cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt
     7861 aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc
     7921 gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac
     7981 tgaagtcagc ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa
     8041 cgactatgta ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga
     8101 gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat
     8161 gactggtcaa gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg
     8221 ggtcgtcatc actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc
     8281 agtgacaggc gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt
     8341 attccaccca gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat
     8401 tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg
     8461 ggaaaattca ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg
     8521 cccactggaa gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa
     8581 cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc
     8641 agcagaaaga gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg
     8701 ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa
     8761 aatgatgcag caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg
     8821 ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa
     8881 gaagaaaaag aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg
     8941 tctactgccg gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa
     9001 cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata
     9061 tcatgatact gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat
     9121 gcttgatatt aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt
     9181 cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat
     9241 cagtgaacta atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt
     9301 ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct
     9361 ttggtcgttt tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc
     9421 aacaacaagt cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt
     9481 tatcggaagc acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct
     9541 tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat
     9601 gcgaaaaatc atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga
     9661 tgatcttcaa tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta
     9721 tgaaacaatc gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt
     9781 ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt
     9841 acaattgcca gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca
     9901 aatgacagaa ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt
     9961 ggaacatcaa atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg
    10021 gattccttta ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg
    10081 attcggtgaa tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag
    10141 gcaactatat gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt
    10201 actctgttaa tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta
    10261 atgttccttc aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg
    10321 cttccggttg ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt
    10381 ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat
    10441 cccaaacact gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg
    10501 gcacagaacg gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct
    10561 cacgaaaacg agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa
    10621 atggcagatc atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa
    10681 aagcttctgc tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa
    10741 ttttttttgt catacgattt ttcctccttc aaactatcta taataatctt acaaaagata
    10801 agtaactgga gcaaataaaa acacttactg acaaaagcta catagaagat agattactta
    10861 tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa
    10921 gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat
    10981 aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc
    11041 taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg
    11101 tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa
    11161 agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa
    11221 catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata
    11281 aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc
    11341 acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat
    11401 ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa
    11461 gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat
    11521 atcatcgatg ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt
    11581 ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat
    11641 ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc
    11701 tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag
    11761 cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac
    11821 tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga
    11881 acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat
    11941 cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct
    12001 cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata
    12061 tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa
    12121 tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc
    12181 aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga
    12241 aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct
    12301 accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc
    12361 cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat
    12421 gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag
    12481 cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat
    12541 aattttggct taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa
    12601 aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg
    12661 taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag
    12721 aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat
    12781 taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc
    12841 cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact
    12901 ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg catgtattag
    12961 aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt
    13021 gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca
    13081 aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg
    13141 atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc
    13201 atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat
    13261 tattcagata acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg
    13321 ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat
    13381 gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca
    13441 gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat
    13501 gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg
    13561 ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta
    13621 gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa
    13681 tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat
    13741 cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg
    13801 cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc
    13861 aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct
    13921 ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta
    13981 cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg
    14041 aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc
    14101 cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag
    14161 ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta
    14221 cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt
    14281 gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt
    14341 gcattacacg gaaaagctgc tcgtatcaaa gaaatcagaa gataaacacg gaaaacttcc
    14401 ttggaaatct gaaatttgtg aaaagtcctt gtatatcaag ggctttttct tttttctcta
    14461 tcacagtttt aagccatttt tggctgtttt ggaccatttt tcaatcatat tcccttaaaa
    14521 attgtcctag attgtccgac attgactatc agtgtcgtca aaatagggca aaatttagta
    14581 gtcagcttga cgacaaatat aatacgaaaa ataccaaaac atttaccgaa tttagcaact
    14641 tttgaaccca agtttctcaa agggtcagct attagcaaaa aagacaaaac atgtactttt
    14701 ctactaaaaa gttgatgtga taggcttcta tctaaataac tattaggagt ttttatcgtg
    14761 aagcaaaatg atggacgtat catttggaaa aatcaaagtg agttaaaact gattttaaca
    14821 attaatgaat tcatcgaaaa acacggaatt acatctagcc gtcaatacca gaaaaagcta
    14881 tcagaaaatc caaattctgc accaagtatg tggttcatta acaaaaaata tggatcatgg
    14941 gagaatttat tgattagtat aggacgagaa aatactgggt atggaaaatg ggctagaatg
    15001 tcagaacaag aattattgga aatagtagaa gcctttatta agtgcgagaa gataacctct
    15061 caacgaatgt acgaacaaaa atcagtaggg aagaatattc cttcacttag taccattaaa
    15121 aagatgttag gagatatacg tccacttttt aaagaaaaaa atgatggatc tcgatttact
    15181 gatttcgaac tgctgttaga actaaaaaat gaaataatcc gattaaaact acaggatgat
    15241 ttatcaatga caaagtttcg aaaactagtt caatccccaa aactaccatc agttgataca
    15301 attatgaaaa gaacgaacaa aaattgggag gaattgatgg cagagattgg atttgattat
    15361 cgaagaatca aaatttataa gcagagaaac aacctatcca agacaaagaa aactaaatag
    15421 tatcactcta aatcagacgt tcaaaattaa aatttggacg tcttttttat tacccaaaaa
    15481 cgaaaggaga tttattttat gaaaaagcca agatttaaga attggcgttt gctcgcaaca
    15541 ctgagtctat tatgccaaac aattggtgga tcgcttggcc caacgattgc ctttgctgat
    15601 gaaattactc atccacaaac ggtaactgtg gaattggatt tggctcacca gtatgctgtc
    15661 gaaggtacat ttagcgatgg tcgtcccatg tcagaagtta ccgttccgca ctatgcagtt
    15721 tacaatggtg tgaagcaaga tattttctgt attgagccag gggtgccgat ttacaatgag
    15781 ttcactcctg gttatgagaa aaatccattg cctgatatgt cggagaaagc gaaattagtt
    15841 tccgttttat ggaaggaagc tggtacggat gtggatacac atattgtggc ccagaagatg
    15901 atttggcaag aagttaatgg ctacacgctt cactcaatta aacgttctga tggtagtgca
    15961 gtaaatatcg cagccattga gtcaaaaatc aatcaagcca ttgcggatta tcaaaagaaa
    16021 ccaagcttcc ataactccac agtgaaaacc gtgttaggtc aatcgactac tgtgacagat
    16081 acgaacggct tgaatttatc ggagtttgat gaagttgtcg agaatatcgc aaatattgat
    16141 tatcgtgtga acggcaatca actagtgatt actccaaatg ccaattcaaa agagagtggt
    16201 gaattaaccc taaagaagtc agctaataca ggaacaccgg tagcttataa aatggctggt
    16261 caacaaactt tgatggcggg ggcaattgat aagcccaata cctacacaat caaaattgat
    16321 gtggaaactg agggttcttt gaagattaaa aaagtcgata aagaaacagg aaatgttgta
    16381 ccgggaaccg ttttccattt agactttgga aaaacattac ctgcaaaaga cgtgaccacc
    16441 gataaagaag gcattgcgac attggatgag attcctcatg gcgcgaaagt aaccattact
    16501 gaaaaatcag tgccagctcc ttatacgatt gacactacac caatcaccgc aaccattaaa
    16561 tctggtgaga ctatttctgt gacttcaaaa aatactcgcg aaaaaggtca aatcattttg
    16621 aataaaatgg gggtggaaac aggaactgat ctttggaacg acaaatactc attagccgga
    16681 aatacgtttg ccattcgcaa agacagtcca accggtgaaa ttgtccaaga aatgaccacg
    16741 gatgaaaaag gtcatgcgga aacaccaaaa gagattgcca atgcgttgga attgggaacc
    16801 tattatgtga ccgaaaccaa agctagtaat ggctttgtga atacctttaa accagtaaaa
    16861 gtcgaattga attatgtcaa tcaaaccgtg gctcttgtta ccagtaacgt aaaagggcaa
    16921 aaccaagaaa ttactggtga aaccactttg acaaaagaag acaaagatac cggtcatgaa
    16981 actcaaggga aagctgagtt taaaagagct gaatacacct tgtttactgt taaggatgga
    17041 aaagcggtca aatggaccga gaactttaaa ccagaattgt tgaagggaac gaaagcttct
    17101 gatgaaaccg taactttgac tttagatgaa aagaatcaag ttgccgttaa acacttagcg
    17161 attaatgagt attactggca agaaaccaaa gcgcctgaag gatatacctt ggatgaaaca
    17221 aagtatcctg tatccatcaa aaaggtggat gatagcgaaa aaaatgctgt gattactcga
    17281 gatgttgcgg caaaagaaca agtgattcgc tttggctttg atttctttaa atttgctgga
    17341 tcagctgctg ggactgccga aactggcttc aatgatctga cctttaaagt atcgccattg
    17401 gaaggaacca gtgagattac aggtgccgaa gatgaagcaa ttacagctta taacgagcaa
    17461 ctaggttttg atggctatgg caagtttgaa aatcttcctt atggggatta tttacttgaa
    17521 gaagtagagg ctccagaagg atttcaaaaa attacgccat tagaaatccg ttctactttt
    17581 aaagaaaata aagcggatta cactaagagt gaatacattt ttacgattac cgaaaaagga
    17641 caaaaacaac caattaaaac ggtgaccgtt ccttacgaga aactgacgaa taaagcattt
    17701 tctgttagct tgaatcgttt gatgctctat gatttacctg aagaagaaga tagcttaact
    17761 tctcttggga cttggaaaga cggaggcaaa gaattgacag accttgattc taccgagcta
    17821 gttgataaat tgagctataa cttgcatgaa atcaaagaag actggtatgt ggtagcgcaa
    17881 gccattgatg tggaagcgac aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg
    17941 gtaattgccg aaacaaccgc aacgttggcg aataaagaga aaacaggaac ttggaaaatt
    18001 ttgcataaat taaccgcaga acaagttttg gataaaacca tcgtcttatt caactatgtg
    18061 tatgaaaaca agaaacccta tgaagcaggt gatgatccag tagcgaaaga tgctagcttg
    18121 aacaatcaag cccaaactgt caattgtacg gtggaacgcc atgtttccat ccaaacaaaa
    18181 gcccacctag aagatggttc gcaaaccttt actcatgggg atgtggtgga tatgttcgat
    18241 gatgtgtcga ttactcatga tgtactggat ggctcaaaag aagcttttga aacaattctg
    18301 tatgcattac ttccggataa tacgaccaaa gaaatttgga aatctggcaa aattgattat
    18361 gaagtgaatg acaaagaatt cactaaaacc gtacttgcgg aaaaagtgga cacaagtaaa
    18421 tatccagaag gtacaagttt taccttcaaa gaaattaact atggcaaaga cggaaacatc
    18481 aatgggaaac acaatgagga tctaaaagaa aaaactcaaa ctttaacacc taaaaaagtt
    18541 ccgacaacac caagtgcacc agaacaaccg gaaataccaa ctgttccaag tgactctcaa
    18601 gaatctagtc ctacagtaaa gacattccca caaacagggg agaaaaattc taatgttctg
    18661 ctgtttattg ggtttacctt gatctttgcg actgctgggt attatttctg gaatcgccga
    18721 aactaaggtg atgtgatgaa acaactaaat aggaaaaacc aacgtgcgcg accgcctcct
    18781 aaaaaaagtg gtcgcgctat ttttgtatcc aaaactaaaa atgaaaagag gaaaagaaga
    18841 tggaattgaa atttgttgtg ccagatatgg ccgaaacctt tggaaagata aggtatgccg
    18901 gcgaaggcga agttttaact gaaggatatg ggcggaatac tacagtaatt ggtcgtagtt
    18961 atcatttgta ttccagtaag caacgagccg atgatattga agtggtggta gctgcggaag
    19021 ccggcgaaaa ggattttgat caagatcaac cactaaaagc cgtgaatccc catttggttg
    19081 ccaaaggcta tgagattgag aatcgtgggt ttaccgacta cgtgttgtat gtcgatgatt
    19141 tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtt gtagattcag
    19201 gactaacgtt tgggaaatta cgattttccg cattacgccg agaagtacgg aagcaaaatg
    19261 aggatggaac ggttagtaac gaggtaaaag aacggactta taatttgaaa tcctctgcgc
    19321 aaggtcgaat gatccaagtt agtatcccag caaatgtacc cttacgcgag tttgcctatg
    19381 acgcagaggt agagttggtc aatccaatca tggatacggt cgccaattat gttttccgag
    19441 aaggaactac tgtcaattgg ttcattaagg ccgatgattt agtcttgaaa cgacaaccag
    19501 atcagggaaa ttctacaaac caaaatgaag gaaagaaata ggtgaagcgt atggaaatta
    19561 atgttgaaca tattttagat tgtctcgatc aatatggaaa aggtgagcta acggaggaac
    19621 aactcactaa agcattaacc tatgatgaaa aaatgtttct gattatgcat caagggttac
    19681 tggaagtagg taacgatgcc gaagaagatt ttgatgtact gaattggtta ggggaagaag
    19741 agtccttctt tatggttatc gaagtaaatg aaagtctatg tcgagaagcc gaaaccgtat
    19801 tggaagaaat tggtgtggaa atgccggatg ccattgaagt ttttttaaag caactagtgg
    19861 aaacgaaaca actccctata gcagtaaatg actagcataa ctgcttgaaa ttggatcaat
    19921 gaaatcctat gaagcttttt tcgattggag gtggaaacca taaaaatgta caaagggcat
    19981 cgtatacggg caggagatca gcacttggtt tatcactttg ttcttggctg gctactcgct
    20041 ctgttcatcg gttggatgag cgtcttttat tttcaagagc ttagacaatt tgatatttct
    20101 aagctatcac tttctactat agaaatcgtt cgttctataa aagatttaat ctatttgcta
    20161 ggaagtctcg ttctttcagg aagcacgatg ttgctttata tacacttttt tcaggatcat
    20221 tggcgcagtt tgtggcatcg acaaaagcta gcccggatga ttctagaaaa tcattggtac
    20281 gaagtaaaac aaactcagag tgaaggattt ttcaaagacc tcaatagtag tcgaaccaaa
    20341 gagacaatca gttacttccc caaaatctat taccgtatga aggatggttt actttctatt
    20401 cgcgttcaaa tttcactggg aaaatatcaa gatcagcttt taaagctgga aaagaagtta
    20461 gaaagtggtc tatattgtga actagtagaa aaggaactca aagactccta tgtggagtac
    20521 actttactgt atgatataat tgccaatcga attgggatag acgaagtagt ggcagaaagt
    20581 ggggctttgc gattgatgaa aaatcaagta tgggcttatg attctttacc tcatatgtta
    20641 atcgctggcg gcacaggtgg tgggaagact tactttttac tcactatcat cgaggcttta
    20701 ttaaaatctg atgccgagct ttttgttctt gatcccaaaa atgcggactt agccgattta
    20761 ggtacggtaa tgcctcatgt ctattcccaa aaggaggaaa tttctgcttg tgtggaagat
    20821 ttctatgagc gcatgatgac tcgtagcaag gcaatgaaag aaatgcccaa ctacaaaaca
    20881 ggagagaatt atgcgtttct tggacttcca ccaaactttt taatctttga tgaatacgtg
    20941 gtttatatgg aaatgttaac gacgaaggaa agtgcggtga ttttgaataa gctcaaacaa
    21001 atcgtgatgt taggtcgtca atctggtttc tttctgattc tagcttgtca aagaccggat
    21061 gcgaaatatt taggagacgg gattcgcgat caatgtgcga catgtttcta actgaaaagg
    21121 ttaggcactt aaaagaagaa aacataagtt tccttttaag gagaactgat attttgagca
    21181 gtggaatgat ggggtaacgc cctgaaacgc tcccttaata ctccgactgg ctgtttaaca
    21241 tatagctgtt agatggtcag aagctcggtg aagtcggctg aaagttaccg taaatatgat
    21301 aaattcatat acgaaagttg ttcagaggtg aatgatgtaa cctataggtc ggaggtctat
    21361 aaaatactca cggtgagaat gtaagtaata ctactgacga acttgcgaat gaaagaatct
    21421 atacacggac tgtatagaaa tgtacaggat tctaccgtat caacggaaca gaatgtatgt
    21481 tgtggtttag taagattatt ggttatgaac aaccatgcaa tgttacaggc attggaaata
    21541 tacaggttca aagcaagcac ctaaaagtat atataaagat aaaaatatcg aaacatggga
    21601 agctggcaat gtggaggaaa ctatattcca ttgaaacatt tgtgacgaag aataataagc
    21661 acatttatgc cagtgaaagt ggtggcacag taccaatgag gatatagaaa tatatcggag
    21721 gaatagccac tagtcaaaag aaatcgttaa ttaaatgatg tatttcatag aatcgagtaa
    21781 gacaaaataa acactacaac ctaagtaaac aaaggtagtg gtgctatgaa taaagtaaaa
    21841 acaaataaaa cacttcgtca tagtgaatac tatggcatga cacaaatatt tgatgaactg
    21901 tatgtaaaaa gtgataaaaa agaagttttt acagagctca tgacaattat tatgagtgat
    21961 gaaaatattc gactagcata cagaaatatc aagaataaca agggcagtta tacagcgagt
    22021 accgataaga aaagtattcg agacattaaa acactgtcag aagatacctt tctaactgag
    22081 gttaaagttc tttttcgctg ttacaaacca cgagcagtta gacgtgtaga aattcccaaa
    22141 ccaaatggaa aaacacgccc tctgggcatt ccatcaatct gggataggtt aatacaacag
    22201 tgtattttac aagtcttaga accgatatgc gaagctaaat ttcacaatta tagttatgga
    22261 tttcgaccaa accgttccac acatcatgcg attgctacct gcaatcgact ggcaaatagg
    22321 gggaagctac actttgtagt agatgttgat attaaaggat tctttgataa tgtcaatcac
    22381 tctaaacttt tgcgtcaaat gtggacgatt ggaatccgtg ataaatggct attaggtatt
    22441 attcgatcca tgctaaatgc tcctatagtc ctaccaaccg gtaaagtcga acaccctaaa
    22501 aaaggtgtac ctcaaggtgg tgtcctatcc cctttactat ctaatatcat attgaatgaa
    22561 ttagattggt gggttgcttc acaatggcaa aataccacga ttgagacaga aaagattatg
    22621 gataggcgag ataaaggcaa aggcattgat aagactaatc agtatcgggc attaagacga
    22681 acaaacttaa aagaaatata cattgttcgg tatgctgacg acttcaaaat cttttgtaga
    22741 aaacgaagtc atgcagataa aatatttcac gcaacgaaaa tgtggcttaa agaacggtta
    22801 tcacttgaaa ttagcgaaga aaaatccaaa attgtcaatc tcaaaaaagg taaatcagaa
    22861 tatctaggat ttgaattaaa gctaatcaag aaaggtaaaa aatacgtagt cgaaagtcat
    22921 atgagcaaaa aatcaatgac aagagttaaa atccaattaa agaaacaatt acgtaaagtt
    22981 gccagaccga agaatcattg cgaacaagca aaagaaattg gattatataa ttctatggtg
    23041 attggaatcc atcaatatta tggaattgca acctgtgtaa atctcgattg cagtaaaata
    23101 gcctatacac taaaaccgtt catcaatcac aaactgccga caaagaaaca tggaaagata
    23161 ttaaatacat ttatcaaatc gaagtatgga aaaagtaaag aaatgcgatg gttgaataat
    23221 gtgccaattg taccgctcaa ctatgttcag tttagacttg ctgtccccct atcagaggga
    23281 acttgcaagt acactgaaag tggacgaagc aagatacaca gtaaattaaa attagaccta
    23341 gcccttttac ttcacatgat gagaaatagt aattatgaaa gaagtattga atttgtggat
    23401 aacagaatat ctaaatattc tgctcaaaag ggaaaatgtg ctatcacggg taaatttctc
    23461 gaatacgaag aaatccattg ccaccatatc gtaccagtaa aacaacaggg tacagacaaa
    23521 tacagcaacc tcattattat tcacaaaaat gttcacgcat tagtccatgc tatcgaagaa
    23581 aaaatcatac acaaatacct gaatgtatta aatctaacca atgaacaaat agaaaaacta
    23641 aatgaattac gtgtcaaagc tggtaatagt gttttaacag tttaacttga aaaatatatc
    23701 atacttaaca ttcttttgat ggaacgccgt atgaagtgaa agtttcatgt acggtgtgaa
    23761 gcgggggaaa atctggagat gacatcaaag gattacctat cgc
//
LOCUS       DAFBQW010000032        23659 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFBQW010000032 DAFBQW010000000
VERSION     DAFBQW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.033"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            257..532
                     /locus_tag="LK889_001673"
     CDS             257..532
                     /locus_tag="LK889_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095908.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="LK889_001674"
     CDS             545..2623
                     /locus_tag="LK889_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBK5095909.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="LK889_001675"
     CDS             2783..4669
                     /locus_tag="LK889_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5095910.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="LK889_001676"
     CDS             4681..5628
                     /locus_tag="LK889_001676"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK5095911.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="LK889_001677"
     CDS             5648..6175
                     /locus_tag="LK889_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5095912.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="LK889_001678"
     CDS             complement(6238..6891)
                     /locus_tag="LK889_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK5095913.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="LK889_001679"
     CDS             7023..7865
                     /locus_tag="LK889_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5095914.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="LK889_001680"
     CDS             8023..8919
                     /locus_tag="LK889_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5095915.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="LK889_001681"
     CDS             8922..9635
                     /locus_tag="LK889_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBK5095916.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="LK889_001682"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="LK889_001682"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5095917.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="LK889_001683"
     CDS             10170..10397
                     /locus_tag="LK889_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBK5095918.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="LK889_001684"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="LK889_001684"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5095919.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11683)
                     /locus_tag="LK889_001685"
     CDS             complement(11183..11683)
                     /locus_tag="LK889_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK5095920.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="LK889_001686"
     CDS             complement(11962..12360)
                     /locus_tag="LK889_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5095921.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="LK889_001687"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="LK889_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBK5095922.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="LK889_001688"
     CDS             13473..14240
                     /locus_tag="LK889_001688"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK5095923.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="LK889_001689"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="LK889_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK5095924.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="LK889_001690"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="LK889_001690"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK5095925.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="LK889_001691"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="LK889_001691"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBK5095926.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="LK889_001692"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="LK889_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK5095927.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="LK889_001693"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="LK889_001693"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBK5095928.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="LK889_001694"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="LK889_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBK5095929.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="LK889_001695"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="LK889_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBK5095930.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="LK889_001696"
     CDS             22691..23566
                     /locus_tag="LK889_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK5095931.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6405 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgattt
//
LOCUS       DAFBQW010000033        23574 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFBQW010000033 DAFBQW010000000
VERSION     DAFBQW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.034"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            45..677
                     /locus_tag="LK889_001697"
     CDS             45..677
                     /locus_tag="LK889_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK5095932.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(679..1584)
                     /locus_tag="LK889_001698"
     CDS             complement(679..1584)
                     /locus_tag="LK889_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5095933.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1669..2328)
                     /locus_tag="LK889_001699"
     CDS             complement(1669..2328)
                     /locus_tag="LK889_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5095934.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            2644..3588
                     /gene="mvk"
                     /locus_tag="LK889_001700"
     CDS             2644..3588
                     /gene="mvk"
                     /locus_tag="LK889_001700"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBK5095935.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3581..4558
                     /gene="mvaD"
                     /locus_tag="LK889_001701"
     CDS             3581..4558
                     /gene="mvaD"
                     /locus_tag="LK889_001701"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBK5095936.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4571..5656
                     /locus_tag="LK889_001702"
     CDS             4571..5656
                     /locus_tag="LK889_001702"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBK5095937.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5653..6717
                     /locus_tag="LK889_001703"
     CDS             5653..6717
                     /locus_tag="LK889_001703"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK5095938.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6966..7769)
                     /locus_tag="LK889_001704"
     CDS             complement(6966..7769)
                     /locus_tag="LK889_001704"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBK5095939.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7789..8631)
                     /gene="kduI"
                     /locus_tag="LK889_001705"
     CDS             complement(7789..8631)
                     /gene="kduI"
                     /locus_tag="LK889_001705"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBK5095940.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(8647..9654)
                     /locus_tag="LK889_001706"
     CDS             complement(8647..9654)
                     /locus_tag="LK889_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBK5095941.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(9661..10305)
                     /locus_tag="LK889_001707"
     CDS             complement(9661..10305)
                     /locus_tag="LK889_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK5095942.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(10405..11163)
                     /locus_tag="LK889_001708"
     CDS             complement(10405..11163)
                     /locus_tag="LK889_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBK5095943.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(11290..12441)
                     /gene="fucO"
                     /locus_tag="LK889_001709"
     CDS             complement(11290..12441)
                     /gene="fucO"
                     /locus_tag="LK889_001709"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBK5095944.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(12523..12834)
                     /gene="rhaM"
                     /locus_tag="LK889_001710"
     CDS             complement(12523..12834)
                     /gene="rhaM"
                     /locus_tag="LK889_001710"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBK5095945.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(12836..13681)
                     /gene="rhaD"
                     /locus_tag="LK889_001711"
     CDS             complement(12836..13681)
                     /gene="rhaD"
                     /locus_tag="LK889_001711"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBK5095946.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(13695..14951)
                     /gene="rhaA"
                     /locus_tag="LK889_001712"
     CDS             complement(13695..14951)
                     /gene="rhaA"
                     /locus_tag="LK889_001712"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBK5095947.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(14948..16408)
                     /gene="rhaB"
                     /locus_tag="LK889_001713"
     CDS             complement(14948..16408)
                     /gene="rhaB"
                     /locus_tag="LK889_001713"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBK5095948.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            16561..17430
                     /locus_tag="LK889_001714"
     CDS             16561..17430
                     /locus_tag="LK889_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBK5095949.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(17556..18455)
                     /locus_tag="LK889_001715"
     CDS             complement(17556..18455)
                     /locus_tag="LK889_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5095950.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(18620..19444)
                     /locus_tag="LK889_001716"
     CDS             complement(18620..19444)
                     /locus_tag="LK889_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK5095951.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(19455..20750)
                     /locus_tag="LK889_001717"
     CDS             complement(19455..20750)
                     /locus_tag="LK889_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBK5095952.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(20766..21245)
                     /locus_tag="LK889_001718"
     CDS             complement(20766..21245)
                     /locus_tag="LK889_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBK5095953.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(21251..22279)
                     /locus_tag="LK889_001719"
     CDS             complement(21251..22279)
                     /locus_tag="LK889_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBK5095954.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(22296..23402)
                     /locus_tag="LK889_001720"
     CDS             complement(22296..23402)
                     /locus_tag="LK889_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK5095955.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
BASE COUNT         6779 a         5200 c         4238 g         7357 t
ORIGIN      
        1 ttcccgcttc tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac
       61 ctattccacc ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga
      121 tcttctctgg tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg
      181 tattaagaga gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg
      241 aatgcagtaa tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca
      301 gtttccacca agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt
      361 atcttcgaga ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa
      421 acttgaaagc tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca
      481 cttggaaaaa cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt
      541 ctctgaaggg attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt
      601 ctttggcggg actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt
      661 tgcagatcaa agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga
      721 taagaccaaa ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa
      781 ctggagattc attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca
      841 gaccgtcaaa aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc
      901 agagagctgg tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc
      961 gatcagcgca tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa
     1021 tgccttgatt tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa
     1081 accgatctct aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt
     1141 cagattaagc cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga
     1201 aatcaaaaac aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa
     1261 aagcgatcga attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc
     1321 gtcttctttt aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg
     1381 gtgcggaaca tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc
     1441 ccgttcattt ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt
     1501 taagttttta acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg
     1561 attaaaatag tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga
     1621 aaaaagtcgc tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac
     1681 tttggtttgg cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc
     1741 ttgaggatct tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt
     1801 gtattcttca taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact
     1861 tggatcaaag gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt
     1921 atcaaaagat acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg
     1981 aagttcttcc atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc
     2041 tcttcctaga tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa
     2101 tacattattg tcatctttca ttgcttgttt gatgcttgca aaacgtttat tcaaaaaata
     2161 aatttgcagc aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt
     2221 atcatcaatc gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa
     2281 acttgatttt ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct
     2341 cacttcatca ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta
     2401 gtagtgtcaa ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga
     2461 aaaaagtgtt ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta
     2521 aaataaagga ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc
     2581 aactcgtttt gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag
     2641 attatggcaa actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac
     2701 gcggttgttt atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca
     2761 ttccttgaag agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat
     2821 ttagccgaag ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa
     2881 cagcatgaca tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt
     2941 ggaatgggat caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta
     3001 gcatttcctt tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc
     3061 cacggtaatc ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt
     3121 acaaaagggc atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct
     3181 gatacaggaa tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa
     3241 acccagcctc atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca
     3301 aaacaagcaa tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa
     3361 tcacttctgg aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca
     3421 aaagataccg gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt
     3481 gcattagcac aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt
     3541 gctgctgaaa cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg
     3601 cgcatatacc aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct
     3661 tccaatgaac aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat
     3721 cttttctgat agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa
     3781 agcaacaaaa aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact
     3841 aaaagcttca gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc
     3901 cagcgggcta gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga
     3961 tctctctctt tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg
     4021 tggtttcgtc gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc
     4081 ttccgattct ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa
     4141 agaagtgtcc agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg
     4201 ctggttagat tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga
     4261 tttccaactt ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt
     4321 agctgcccag cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt
     4381 gcgacaattg agaaagcaag gcattccatg ctactttacg atggatgctg gccctaatgt
     4441 caaagtttta gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt
     4501 tagcaaagaa caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag
     4561 ggagattttt atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc
     4621 agttgttgaa acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac
     4681 tgtagaatct gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt
     4741 acgttggaca agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta
     4801 tatccttgct gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc
     4861 cttttatgat ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt
     4921 gggttctagc ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt
     4981 gaatttatct cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga
     5041 taatggttcc tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac
     5101 cttcgaccat ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc
     5161 cctggattgg ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt
     5221 gattggttgg acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc
     5281 gagagaagat aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa
     5341 cgaaatgatc aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa
     5401 aaaccgacaa ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc
     5461 tttgaacaaa ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc
     5521 aggtgggggc gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat
     5581 gagtgcatgg gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag
     5641 aaaggaaaac cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat
     5701 aaacaaaaaa acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca
     5761 gtttctgatg tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat
     5821 atcaatgcga tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc
     5881 gtcgcaagag aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc
     5941 ccttctttag ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt
     6001 gccaacatcg gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac
     6061 gcagatgcct tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac
     6121 cgtgatttta ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc
     6181 ctcatcgtaa aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct
     6241 ttaggagttc atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa
     6301 aacgcccgtc gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc
     6361 tcttcattgc ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt
     6421 atccgcaatg cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact
     6481 tcaggaaccg tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg
     6541 aagcaatggc aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg
     6601 gccctacacc aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga
     6661 gggatcgact tagtgaagta tggaagacgc acagaaaaga gcgtgggaca aaagtaaaga
     6721 acacttttgt ctcacgctct aaacacgtac aaacggcgga agtaaaagca actccttcgg
     6781 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaattcct tcttatttct
     6841 tggagttaaa tgcttctgtc ccagcctctt ttttcgaata aatgatgttt aatcagctgt
     6901 ccttcggtat tcgttcaatc cgctgatttc acaacctctc cactaaacag tgtactaaag
     6961 ctcttttaag gttgttttcc gatataggca agtatgcctc cgtccacata caagacatgg
     7021 ccgttaacga aatcagatgc gctagaagcc aagaagacag atggtccagc caaatcgatt
     7081 ggctcgcccc agcgtgctgc tggcgtacgt ccaacgataa attgatcgaa tggatgacgt
     7141 tcaccgtttt cttgtgtttc gcgtaatgga gcagtttgcg gtgtagcaat ataacctggt
     7201 ccaattccat tacattggat attgtattga ccgtattcag aagcgatgtt tttcgtcagc
     7261 atttttaatc cgccttttgc cgcagcataa gcactgaccg tttcgcgtcc taattcactc
     7321 atcatggagc agatattgat gattttaccg ccgccttttt cgatcatatc cggaataact
     7381 gcttttgaaa cgatgaatgg ggcatttaaa tccacgtcga ttacttggcg gaagtctgcc
     7441 gcatccattt cgatcatcgg tgtccgtttg atgatccctg cattgttgac tagaatatca
     7501 actgaaccta catcttcttt gatctgttga accatcgctt gaaccgcttc ttcatcggtc
     7561 acatcgcaga cataaccttt tgcatcgatg cctgcttctt tataatgcgc tataccttca
     7621 tcaactgatt cttgattcaa gttattaaat acgatttttg ctcctgctgt agctaaagag
     7681 cgtgcaatct caaatccaat accatagaca gcaccggtga ctaatgctac ttttccatct
     7741 aaacgaaaca tatccatact aaattccatc attttctctc ccttatgctt attttaattg
     7801 atccatttgc accatatcca tgtctgtata ggtgatattt tctccgcaca ttgcccagat
     7861 gaatgagtaa ttgcttgtac ctacacctga atggatcgac cagcttggcg aaataatggc
     7921 ttgttcattg tctacaacta aatgttttgt ttcatctggt ttgcccatca tgtggaacac
     7981 acgtgtatct tcattggcat aatcaaagta gacataagct tccatacgac gttcatgtgt
     8041 atgacaaggc atcgtattcc aagcgcttcc tggttctaaa atcgtataac ccatttgtaa
     8101 ttgacagctt tcgcatacat ttggatggat gtattggtag attttacgtt cattcagtgt
     8161 caggccttca ccagtctcca ttggttttac ttggtcgata ctgattttta catttggata
     8221 tttatgatga gctggaacag aactaatata gaatttagct gggttttcag catcttgaga
     8281 agaaaataca acatgtcgtg tttcttttcc gatatagtat ccgtcttgtt ttttcatttc
     8341 ttctttttgc ccatcgatct cgatgaatcc tggtccaccg atattgatca cacctagttc
     8401 acgacgctct aagaagtaat caacacctag ttctttgtct aatgtgattt ctaattcttt
     8461 ttcagtaggt gtcactccac caaagatcat tcgatcgttg tgcgtataag tcaaactgat
     8521 tttccctggt tcaaatacct tttctactaa aaattctttt ctcaattgtt ccgttgaata
     8581 atgacggata tcttctggac tgtgtgtata acgtgtttcc atttcttgca ttttcttcta
     8641 ctccttctat cgaatgattt ttccagagcc gcatgctaaa aattgtgcga cttcttcttg
     8701 actaaattgg ttgcagtccc catggatcgt atgcttcaag gcagaagctg ccgtagcaaa
     8761 atcaatgatt tcttgcggag acatattgga taaaatccca tggagcacgc cacctgcaaa
     8821 agcatctccg ccacctactc gatccacgat tggggtgatt ctatggactt gggattccac
     8881 atattcatgg ttcatccaca atgtcccctt caattcattt tcacttgctg aatgaacggt
     8941 acgcttggtc gaataaaaca cctgaatatt cgggaacttt tcctgcattt tttgataata
     9001 ataaattaga ggttgttctt ctgcttctgg cgctttctcg atacctaaca aataaagagc
     9061 atccattttt ccagctgagc agatatctac taaaggtaaa agctgatgga ttacttcgct
     9121 ggcttcttgc tgtgtccata attttccacg atagttgata tcgaagctga tcaggcagcc
     9181 agcttttttt gcagcttcta tcacctgttt cgtcattttt tgccattttt tagacaaagc
     9241 tggggtgatt cctgaaacat gaaatatatc tattccttgg aataactcct ctagcgacca
     9301 ttccaaatga tccatttcag caaaacttga tccggcacga tcatagatca ctgaggcggc
     9361 gcgttcgcca ctaccagttt ccatataata agtacctagt cttgatccac cagataatac
     9421 ctgtttcgta tctactccat aacattgcaa gtgctttttg actgcttttc ccaatgcgtt
     9481 atcaggtact tttgtagcaa aaatcgcttc atgtccgtaa ttagctaaag aaattgctac
     9541 gtttgcttcc cctccgccat aatgggcagc gaatcgttca cttgaatcga tacgggttcc
     9601 ctgctctgtg gacaatcgca acatgatttc tccgagtgtc actacttttc ccataagcag
     9661 ctacctcttg atttctttca tttttgctgc atactgttgc gcgacttccg ttactttgtc
     9721 aaaatctcct gtagctgcgg gtgctaaaag gttcccacct actccgactg ttacgacacc
     9781 agcatcgaac cactctttca tattgtccag actcacaccg ccagttggca tgatattgac
     9841 ttgaggcatc ggcgccttga aggcagaaat gatacttggt ccataagcgc ttccaggaaa
     9901 tagtttgacg atatctaccc cgcttttcag cgctgttttg atctcggtaa tagtcataca
     9961 tcctggtaag taaggaattt gatacagatt gcacatctct gccgtttctt gatcaaagga
    10021 agggctaaca atatactctg cgccagctaa gatagccaag cgagctgtga cagcgtctaa
    10081 tactgtgcct gcgccaatca ctatttctgg ttggtctttg tagaatgaca aaagttcttt
    10141 gattacttga tctgcttgag ggactgtaaa agtcaactca ataccagtta gaccaccttt
    10201 gatgattgcg tggcaagcat tcaaagcttc ttctttgctt tttccgcgta ccaccgcaat
    10261 cactccggcg tttttcaatc gagataaaat atcaattttt ttcatcttct ccacctattt
    10321 cctatttaga aattttattt tataacttgg aaatctatac ccatattata cggcagaaaa
    10381 gaaaaattgc aaacgctttt ttcattattt ttgaattctt tgctcgattt cgctttttgt
    10441 ttgcttgagg gcactgatta tttgttcttt tgtctcatct gttaaacgat atttcggcat
    10501 acttacacta aatgcaccaa tgatttgttc gtttttcacg attgaggctc ctatacaaaa
    10561 aatatcacgt tcgatttctt catcgtcaaa tgcgatgtgg gtttctttta ccgtttggat
    10621 ttcctttttc aaccgtaatg cattggtgat cgtatattct gtgtaaggga tcaacggatg
    10681 cttttcaaga taatcagtgt actgctcttc actgaattct gctaatacgg ctttgcccat
    10741 cgcagaattg tataatggtc gggtgattcc gatttttgaa gacatgcgga tcgtttgatg
    10801 ttttggatct agtttgttca cgtacaagat ctcattgttg tccaataccc ctaagtggat
    10861 agtctcatcg atcttttgct gcagctgttc taaaaacggt aaagtcaact ctaccaaatc
    10921 gatttgttca atgtttttat tcgcgtagcg aataagcttt gcacctaaac gatagttttt
    10981 ctctgaattt ttatttacgt agccgatcat caacaaagta tctagtattt tcaagactgt
    11041 agaagaggtc attcccacac cctttgcgat cgtctgcaaa gagacatctt ggttttccgc
    11101 taaatagtct agtattttag aggctttgat caatacagtt ccataaggct ttttattttc
    11161 catataatat tctcctattt ctttataaga aaaattatag cggaaatacc atcaatttaa
    11221 aagagatatc cagatataaa ctaccatttc tctttacagg caatttaatg ttgaagatcc
    11281 gcttcgtttt tacatcaaac tttgatataa agcaatgatt tcttctactg tcgtttctct
    11341 tggatttcct ggtgtgcata catctcgcaa agcatcttct gcaattgctg gaatatctgc
    11401 ttcttttaca cctaattcag aaatggtttc tgggattcca accgctttgc tcaaccgatc
    11461 gatctcacca caagccgctt ctcttacatc ttctaaagac atttcagctg ctcctttaat
    11521 ccctagaaca agagcgatct cacggtactt ttctccagta tattctttgt tatatttcat
    11581 gacagttggc aaaagtgctg cacacgctac gccatgagga atgttgtacc atgcacttaa
    11641 cgggtgagcc atcccgtgta ccagtcctaa accgacattt gagaagccca ttcccgcaat
    11701 atactgtcct aatgccattt tttctcgccc attttgatcg ccatctacag aagcttccaa
    11761 agaatggccg atgatttcga ttgcttttaa atgcagcata tcagtcattt cccaagcgcc
    11821 ttttgtgata aacccttcaa tagcatgcgt catagcatcc atacctgttg atgcagccaa
    11881 ttttttcggc attcccatca tcatatcgct gtcgatgaac gcgacgattg ggatatcatg
    11941 aggatcaaca caaacaaatt ttcggtgatt tacttcatct gtgatgacgt agttgatcgt
    12001 tacttctgct gctgttccag aagtcgttgg tatagctaat ataggtaaac atggattttt
    12061 cgtatcagca acaccttcta aactaatgac gtctgaaaac tctggattcg tcaaaataat
    12121 cccaatggct tttgccgtat cgattggcga acctccaccg attgcgatca aacagtctgc
    12181 ctctgcctct ttaacaaaag ccactccatc ttttacattt tggatcgttg gattaggtac
    12241 gatcccgtcg tataaagcat aagcgattgc tgctttatct agaagttctg ttacttttgt
    12301 tgctataccg tgctcgatca atcctttgtc agtaatgaca gctgctttgc cgaatcctcg
    12361 ctgctgaaat tcagggacga tcgactcaat cgctcctttt ccaaaatacg atgtttcatt
    12421 taaaatcatt cgattactca ttttctattc ctcctaattt gtattatttt tcacaataaa
    12481 aacgaaagaa ccccaagcaa ccggttgccc agttgcttgt tgctacagtt caaagactgg
    12541 catcaattcc caactgactg gagaggcatc tggatttgtc tccatgatat cttccatgta
    12601 cttccaccat ttttgattga tagatgtatt tgccattttt tcccataact tttcatcttc
    12661 gatcactaag taaccaaata acaaacttgt actcggatca agatagatcg aatagtcgat
    12721 gaccccgtgt tcacgaagtg ctgtacgcat ttctggccaa agttcatcat gtcttttttt
    12781 gtattcttct tgtttttctg ggtaaacttt catgcaaaaa gcttttttca ccatgtcaga
    12841 cacctgcttt cgtatgaact tcctccaaat aacctgcttt tggagtgaca ccaaacgcat
    12901 ctgctaaatg ccacaggttt tcgtccgtga ttgtttgcaa gattggtcct tgcgctttga
    12961 cgtacgtata gacttctgct gccttttctg ctgtctcgat caaaccaaag acttcatcca
    13021 tgtcttttcc tgacccgtag attccatgtt gtggccataa aaccaatcga gtttctttca
    13081 ttttttcagc tgttgcttcc ccaatctcat ttgttcccgg aaccatccac ggaatgatgg
    13141 aaattccttc tggaaaaaca actaagcatt ccgtacacat ttgccatagc gtacgactaa
    13201 aggaacgttc gtctaattca tgcgtaaaac tcatcgctaa tagatgacta gcatggcaat
    13261 gcataacgat ccgattatct gggtctactt ctaaacgggc gatatgactc atgaaatgac
    13321 ttggcaactc gcttgtagga aggcattcgt tttccagccc ccacaaaagt tccagtgttt
    13381 tgccatcttc agcaactcga acgattccaa gattctcggc aggttcgctc gctacatttt
    13441 taaaatatct tcctgaacca gttacaataa aatagcatcc tgctaaacgg ctggcatcaa
    13501 aaatcatcga gatgcggcgg atcacttttt caggatccaa gaacggtgtg atctcttctt
    13561 cttttagtaa ataagaaata ttcccgccat ttctttcatc ccatcccaag cgataaagat
    13621 tcgacgtcgt ttcgatcatt tctttcacat aaggcgcttg taaaatatct atttttttca
    13681 atatagccac actcctattt tcttaattcc aacacgtctt tttgatattc ctggacttct
    13741 tccagccaat ccaaaccaac tggaacttga tttttgtaac agaaataatt ccatacatcg
    13801 gcatacggga aatctttcaa ttcttccgta taagccattc ttgaagtaaa gtcaaaagcc
    13861 agctccattt ctttcaatcg ctcaactggt tccagcatag ctttcagcag tgctttttgt
    13921 gtatttctcg taccgatgac ccaagcagcg atacggttga tcgttgcatc aaagaaatcc
    13981 agaccgatat ttgttttttc caacaattcg ttacggacta attctttagc gatttcctgc
    14041 aattcatcat ccatcaaaac cacatggtct gaatcccaac gcactggacg ggaaacatgc
    14101 aacatgattc ctttgctgaa taaagctaat gaagataatt tattggaaat cacttctgtt
    14161 ggatgaaaat gtccagcatc tagacaaaca agtttgtcac gagtcaaccc gtaacccata
    14221 taaaattcat gggaaccgac tgtataagct tcagcgccta aaccaaatag tttgctttct
    14281 actgcatctt gtacataccg ttcatctaac tcttctgtaa aaatttcatc gagtgattcc
    14341 attaaacgtt cccgtggggt aaaacgatca atcggattat ctttcatccc atcaggtacc
    14401 caaaagttat tgatacttgt ttgcccagtt tctttcccaa agtactccgc gattttgcgg
    14461 cttcttttcc cgtgttcgat ccagtattca cgaacttctt tatctggatg agccaatgta
    14521 aagccgtctt tcatcattgg atgcgaaaag aaagtcggat tgaagtcaag accgatttct
    14581 tctttttttg cccactctac ccatttttca aaatgtttcg gctcaatttc atttagatcg
    14641 acagattctt ctgtatccaa atagatagca tgaaggttca atttgtgttt gcccggtatc
    14701 aacgaataag ccttctccag atcttgtcta agctcttcag gtgttcgtgc ggctcctgga
    14761 aaattccctg tcgccatgat tccaccggtc aattcgccat ccggtgtcaa gaaacctttg
    14821 acatcatctc cttgccagca atgcatagaa attttgatgt cctgtatctt ttctagtaca
    14881 gcatctgtat ctacaccgat agttgcatat ttttcttttg cttgttcgta atttctttca
    14941 atcgtgttca tacgattact cctttgattt tattcacgta ccattccatc gtgttttgct
    15001 tattttcatc tgggtaaaaa gtttcacatg gaaacgactg tttgattaca tttcttgctt
    15061 gttctatatt cgaaaactga cctgtactga gcatttgcat ggcaacattc ccgattgctg
    15121 tggcttcact aggtcctgct tctacaatga ttccggcata atctgctgtc aattgattca
    15181 gtagacgatt attcgatccg ccccctacga tgtgcagctt ttccaatttc gttccagtta
    15241 agtgttctac tttgatcagc tcttgtgcgt aacaaagagc aagattatca tacacacaac
    15301 gagcgatagc tcccgatgag acagggactg gctgctctgt ttcaaaacag tattgctgaa
    15361 tctcttctat catatttggg ggattcaaaa atcgagggtc attgatttcc atcacttgct
    15421 gcagcggttt ttcttttgca gcaagctctg ccaattccgc ataactgtac tcgtactctt
    15481 gcattcgggc aacttcctgg atcaaccaca tccccatgat gtttttcaaa aaacgcaccg
    15541 tgttatgggc accccattca ttcgtataat tttcagaaaa agcattgcga gaaacattgg
    15601 cgatctttgt ttcaatccct aaaagcgacc atgtcccact gctgatataa ccccaacgtt
    15661 ctccttgacc tggcgttcct aatacagctg acgccgtatc atgacttgct actgtgataa
    15721 aggtcacatc tggcaaatca tattttgaaa attgttcttt ttgcagatct cctagtatcg
    15781 ttcccggctc tgtcaatggt ggaaataatt ccttcgagac accgattttt tcaagaagtt
    15841 ctatttccca ttcttttgtc cctgcattca gcagctgcat agtagatgca ttagttttct
    15901 ccgtcaccat gttaccagta aagacatacc ctaaataatc aggtatcagt aacaatttat
    15961 gtgcttttgc caaattttct ttttcttcag caaacaattg gaacaaggta ttgaaaggct
    16021 ggatctgaat ccctgtctgc tcatacaact tatctagaga atagatttca gaaaactgtt
    16081 ctactgctcc attggtacgc ttgtcccgat aagcaatcgg atcatttatc cgatcccctt
    16141 gttcatcaat cagacagtaa tcgactgccc atgtatcgat ccccgcaaaa aggcgatcaa
    16201 tccctgtctg ctttaatttt tcaaatccaa tcaaaatctc tttgataaga taatcgatct
    16261 tccagcgatc gtgaccgtct ttgttccgaa agccgttttt gaaccggtgt acttcttcca
    16321 agatcaaaga accttttgca tccctcgtac ttttcattag tcgtccgctt gatgcaccga
    16381 tatcgatggc aacaaatgtt ggattcattt ctccacctct tttactttat tgttgattag
    16441 cactttatta ataaagcgtt atcatttatt aacaatgcta tcataataca gaatcttata
    16501 ttttgcttca cactattttg gtatataatt gcattatctt gacaagttgg gagtgtttac
    16561 atggattact gggaaaaagc caaccactca tggtcggaag actcaaaaag agtgatcttg
    16621 acgcctacac aaagaagcag gaatttattt ttttatatcc aagaaatcgg ttacttcaaa
    16681 gcaccaatcc cttactatac tgaacgagca catttacctt cttacttgat aaagtttact
    16741 ttgtctggca aaggttcgct tacatataat gataatcagc aacagctaaa agctggagac
    16801 ttatttttta ttgactgcca agaatatcag cgttatcaaa cgatcagcga agaaccatgg
    16861 gaaatggatt ggatccattt gaatggtcag aacgtccaat cattttatca ggagttcaca
    16921 aaagacggcc ataacacctt ccattcaaca ggggatccat cagaaaacaa gatccaccat
    16981 ttgatcacag aattgatcca catgcaacaa ggcacgaatg cccgtaccga tttccagtcc
    17041 tctgtagcta tccacgagtt gctgaatgaa ctgattttgc aaaagtatca gctagatttc
    17101 gaagaagagg atattcccgc ctacgtttac aaactgaagc aatacttaga cgaacatttc
    17161 aaagaaacga ttacacttga tcaattagaa caacaattct tattgaataa atatcagctg
    17221 agcaaagatt tttccaaatt tatcggttct ccaccaatcg attatttgat cagcaaaaaa
    17281 ctttcttacg ctaaagacct tcttcggtat acagaattta cggttcgaat gatttccgaa
    17341 gaaataggga ttgagaattt tgcttacttt agccgcctgt tcaaagcaaa aacaggacta
    17401 tcgccgagag actttcgtaa attcagctga ttcatataat atgtacgtca ttttttcaaa
    17461 atattttgaa atatatagat aaaaataaaa taaccgcagt ttctgcttct atgtaaacaa
    17521 cagcagctac tgcggttatt ttttagtgat cattctcatt gttcatcttc aaaatgttca
    17581 taatgaacca actgtttctg tgtatcacgt ttcaaaaatt ctttaggcgt tatgccgaac
    17641 tctctcttga atgctttcag aaaatgcgga taagaagaaa aaccactttt actatataca
    17701 gcactagagc tgcggtattc tcgcaacaag tgcttggcat agatcagctt tttcttcatt
    17761 acatactgat gaaacgtaaa tccagtgtac tttttaaatt ctcgtgtgat atggtatttg
    17821 ctgacaaaaa actgcttttc catttcctct aaagaaagcg gatgagataa attttttgag
    17881 atatattgta acatttgttc cacacgctca ttaggaacta cagcattctg ttcttgcgca
    17941 ctctcttctt tttgtacaac ttgattgaga aaaatcaagt attctaccag agcttgttca
    18001 tattttatgt cttcaccgta aattttttta tcaggaggat ggtcgataaa tgataatttt
    18061 tgcttcaata cttcctgttc tatttgcaat acacgactcc tcttctctcc cattggttga
    18121 aagcaagccg atagattaga ccatggcgtg gaacgacttt ctagaaaaga aggggtgaca
    18181 aagatataga cacgctcgaa tacatctgaa gattggcgga cgatacgatg caaatcattg
    18241 gaatggatga gcagcacact tcctgctttg atcgtaaatt gtcgtccgtt caaataaaaa
    18301 gtcgcttctc cctttaatgt agcatggatc tcatagaaat catgatgatg atagatcgtt
    18361 ttattttggc tgacgacatc tttgacatga tagatttcat actgatcaga aaccatcgta
    18421 ttgacttgtt caccgctgat tttcttttgg agcatgtgcc gcacctcctt ttgttcactt
    18481 atgcacaatt ttattatacc acgttttttg taatcaaaaa gagctgcttt tatatactct
    18541 tataaaaaag aagagacgtg gcatcttcta gccccgcccc ctccattcta aatattagtt
    18601 aaccaacgtc ttctttacat tattcaagaa gacttcttcc ataacgaatc ccatcatctt
    18661 ttgtttcttc aagaaccaca tacaggtacg gacgattctt catgatgatt tctttgattt
    18721 ttgtgtactc gatcacaccg tctcccaaat ttaccggcac gattttttcc tgttcgacac
    18781 gaaaatcttt caaatgaacc gcacatatct tttcaccaaa acgttcaaaa gcttcttcta
    18841 ccaattggac ttgttcttga taatcactac ttgtgatcaa attggtcgga tctaaaatga
    18901 ttcctagata gtccgaatct actgcttgga tcagctgctc tacccttgct aaagaataca
    18961 ccggatgatt caaacctggt tctatcccca ccatcatttg gtatttttct ccagctttta
    19021 ccaagcgacg gataacggac acagcttcag caaatgcttc atctgtgaaa ttttcttccg
    19081 tatattggat ttccggcaaa acacatccag tttccgaagc aaccatcgac gcaccaaagg
    19141 aagaagcata gcgaagatac tgctcgaact ttgtcagcaa ctcttgtcgt atcgttaaat
    19201 ctggatgaat catattgata tagcaactta aaattcctac ggtcacttct tgcttttcta
    19261 aaactcgttt gacataattt cccatcccag aattgatctc atttgcgcca gaaggtattt
    19321 caggaaacga aagacctaag gcgagctgca gtgtatgaat cccttgttct tttgtttttt
    19381 ctgcaagatc ttctatcgtt tggacttcgg aaagatcatg tcctctaatt gtaatgttca
    19441 acataactat tctcctaact cagcatattg ggtagccaca tgacgatttg cgggaagtaa
    19501 gtaatcaaaa acaagacgac taagataacc ccataaaaag gaagaagcgg tttcagcatt
    19561 gcctctgctt ttacgcctcc tacacttgca ccgacatata gtcctgtacc tactggcggc
    19621 gtgatggagc ctacacacag attcatgatt gaaaacaacc cgtaatgtac aggatcaacc
    19681 cctacacttg tgacgattgg taaaaatatt ggtgtaaaaa tcaagatagc aggcgccaca
    19741 tccataaaca tacccaccag taataacaca agattgacta ataacagaat aataaattta
    19801 ttatccgtta cgcccataat caacgaacta attgcctgag gaataccggt aaacgccatg
    19861 gcgaaagaca tcatggaaga agtagccaat aagaacatga tcgtccctga cattgctact
    19921 gattgaaaca acatattagg tatatctttt agttttaccg ttttatagta aaacatagca
    19981 atcaacaggg agtaaatgac agctatcgct gaagcttcta ctgctgtaaa tatccccgtc
    20041 aagataccac cgataatgat gacaatcaat agaaggctag gaatcgcctc ttttattatt
    20101 tttccagcat gagccatctg tcctttttcg acaacagggt atttattctt tttagcaacg
    20161 atgaatgcta ctagcatgat tcctagcgcc cacaaagcgc ctacgagata acctcccata
    20221 aacaaagcag agatcgaagc accaccagca acaagtgaat acatgataaa agcagtactt
    20281 ggaggaatga ccatacctgt cggagctgac gcgatattga ccgctgtcgc aaacttccgg
    20341 tcatatcctt cttccacttg ctgagggatc aacactccgc caatcgcggt agaagcggca
    20401 atggccgagc tagagatgga accaaataaa gcattcccta acacattcgt atgagccaaa
    20461 gcccccggca cgcgcccgac gaacaacatc gcaaaatcca ctagtttctt agctatccct
    20521 ccattattca tgataatgcc cgagagaacg aaaaacggaa tagcgatcag cgaaaaacta
    20581 ttgatacttc ccaccatttt ttgagctgaa ctaaaagcag aaacatcaaa cggtataact
    20641 aacaataaag tcatcatcga tgagaatgca atacttatag caatcggcat ccctaataca
    20701 agcaacacaa caaatacaaa aaataacact aatccggctt ctattgccat tttattcctc
    20761 catttttata ttttttcttg aattgctggc ttgttgctta atgtatcgat tagattgatc
    20821 aggctataga taatgatgag tacccccgat acaggtactg acagataaac atatcccata
    20881 ggaatactta aagaaggcga gatctgcccc attgtcaatg tgacagcatt ccatccgcca
    20941 tacaccatga tcatcgcagc aaataccaga aataaacttt gaacaacaac ttctaaaatt
    21001 ttactttttt tgccagttaa tttttcactt aaaaccgtta cggctaaatg tgctttttgt
    21061 cccacaacat acgcggaaga caacatggca aaccagacaa gaccgaatcg aacaagctct
    21121 tccgtgaccg tatttggatt tcctaaaacc gttctggaaa atacttgcca taaaacaaca
    21181 gcgaccatcc caacaagcaa aacagctccc aggatctcta ataccttgtt taaaatcatt
    21241 ttcatcacag ctacgctcct tgctcttgga tcttttgata aaggtctttg taggtaggat
    21301 cgtttttgaa tatatcatga agtggggcaa ctgcctctcg aaacggtgta ttatctacat
    21361 cattgaattg gactccatgt tcttcttgtg ctaaagcttt ttcttcttca attgcttttg
    21421 caaaagcttc aatttcaaaa accgttgatt ctttcgctgc ttcttcaatg gctttttcct
    21481 gctcatcagt tagctgttct ttgatggcat cattcatgat gatgatatct ggcacacgtg
    21541 tatgctcatc ataggaatag tatttagcca caccaccatg tccagctgta tacaagacaa
    21601 attcattgtt ttctgatccg ttgatcaaat tggattgaag agacgtgtac acttcatcac
    21661 ttcccatagg tacgggcgaa ccgcctaaaa gctctaccat tttgatagcg gtttcgcttt
    21721 gcatcacacg gatcttcttt cccttcaaat cttccggcgt atggatcgga ccgtctacca
    21781 tataaaagct gcgctgacca gaatcataat aggtcaatcc ggtaaatcct aaatttttgg
    21841 tcgattggta gattggctcc attatttctt cattatccat caccttaaag aaatgttctt
    21901 catcattgaa tagataagga actgaaaaaa tcgaataatc tttagagaaa ctttccaacg
    21961 ctgaggcact tacttttgtg aaatctactg ctcctgactg tgtcaattcg atcagttcac
    22021 gttctccacc cagttgtcca tctgggaaat actggatctg tacggaacca tctgtttttt
    22081 cttttactaa acgcccaaat tcattcattg catcaattac tggctggctg ttgcttgcat
    22141 aagcaaagcg caaggtaact atctcattta catctgcatc tgcgacagtt gccacactag
    22201 aattatttcc gcagccgctt agacctagca gacctaggct aagcgaactt gctgcaaaca
    22261 gcagtgatgt ttttttcata tgggtttcct ttcttttaac aataagagac gagcaattct
    22321 gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg ctgtgattcc gatattccga
    22381 taaaaatcta aactgtcccc cattcctgtc ccgattgaaa cgttttgaac ttctcctttt
    22441 tcatcgattt gttcaagcaa tccttggata gcacgataag cgacttcttt atattcttgt
    22501 ggtaaataac gtttatggac cgctttcaaa ataccgtaag caaatcctgc agttgcagaa
    22561 gattctacgt aagaagaagg atcatccaac aacgtatgcc ataaaccgct ttcagactga
    22621 taagttttta atgcacgcac ttgtgcttca agtgtttcga tcaataattt gcgcaatgca
    22681 tcttctttcg ataattctaa gatttcaatg atctctggaa tagcaatcgt caaccagcag
    22741 tttccacgag cccaaaatgc ttcggcatag ttatgatttc cttcaaacgt ccagccgtga
    22801 taccacaatc ctgttcgttt atccatcaag tatttgatat ggatcataaa ctggtaacga
    22861 gcttcttcta aatattctgg gcgattcaac agtttcccga tttttgccaa aggaagcact
    22921 gtcatcatca atgtatcgtc ccacaactga ttcttatttt ccggtccata tgtggcatgc
    22981 tgtaacccgt cttcatttgt ccgaggcatg tcattcatga cccattctgc ccattgttct
    23041 aaataaggca aatagcgtga atctttcgtg tcttcataca agtaagccat agtcaatagc
    23101 ggtgccattg tattgacgtt tttgggcgga gcgccttctt ccatacgatc ctcaaaccat
    23161 tcattgacca cttgataagc gcgaggattt tttgtcatct ggtaattttt ataaatacca
    23221 tatagaccga caccttgtgg ccaattccac actgtccagc ttttgtcatc tactttcagt
    23281 ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga tcgttgtcag gttttcaacg
    23341 agcaggtcaa ttttttcttt cactaattct ttaggtacaa aaatctcttc tttaataatc
    23401 atctttacgc cactttttta aacgctttca ttttatatcc ttaatatagc agtaataccg
    23461 tccactataa atatcgcttt ttgaacaaaa actgtctaaa attgacctaa atgttaaata
    23521 acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa cttggagaaa caac
//
LOCUS       DAFBQW010000034        23571 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFBQW010000034 DAFBQW010000000
VERSION     DAFBQW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.035"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LK889_001721"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LK889_001721"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK5095956.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LK889_001722"
     CDS             complement(1172..1867)
                     /locus_tag="LK889_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBK5095957.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LK889_001723"
     CDS             complement(1880..2596)
                     /locus_tag="LK889_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBK5095958.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="LK889_001724"
     CDS             2926..3531
                     /locus_tag="LK889_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBK5095959.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="LK889_001725"
     CDS             3856..4179
                     /locus_tag="LK889_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK5095960.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="LK889_001726"
     CDS             4361..5731
                     /locus_tag="LK889_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBK5095961.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="LK889_001727"
     CDS             complement(5861..7162)
                     /locus_tag="LK889_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK5095962.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="LK889_001728"
     CDS             complement(7392..9350)
                     /locus_tag="LK889_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBK5095963.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="LK889_001729"
     CDS             complement(9527..9793)
                     /locus_tag="LK889_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5095964.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LK889_001730"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LK889_001730"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK5095965.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LK889_001731"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LK889_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK5095966.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="LK889_001732"
     CDS             13137..13700
                     /locus_tag="LK889_001732"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK5095967.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="LK889_001733"
     CDS             complement(13953..14474)
                     /locus_tag="LK889_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBK5095968.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="LK889_001734"
     CDS             complement(14477..15319)
                     /locus_tag="LK889_001734"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBK5095969.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="LK889_001735"
     CDS             complement(15312..16037)
                     /locus_tag="LK889_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK5095970.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="LK889_001736"
     CDS             16213..16509
                     /locus_tag="LK889_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095971.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="LK889_001737"
     CDS             17077..18669
                     /locus_tag="LK889_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5095972.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="LK889_001738"
     CDS             18682..19422
                     /locus_tag="LK889_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5095973.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="LK889_001739"
     CDS             19590..20939
                     /locus_tag="LK889_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5095974.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="LK889_001740"
     CDS             complement(21067..21657)
                     /locus_tag="LK889_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK5095975.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="LK889_001741"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="LK889_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK5095976.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DAFBQW010000035        23362 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFBQW010000035 DAFBQW010000000
VERSION     DAFBQW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.036"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..660
                     /locus_tag="LK889_001742"
     CDS             <1..660
                     /locus_tag="LK889_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5095977.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSDGQKIDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
     gene            1102..1689
                     /locus_tag="LK889_001743"
     CDS             1102..1689
                     /locus_tag="LK889_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095978.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            1689..2588
                     /locus_tag="LK889_001744"
     CDS             1689..2588
                     /locus_tag="LK889_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBK5095979.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            2588..3502
                     /locus_tag="LK889_001745"
     CDS             2588..3502
                     /locus_tag="LK889_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095980.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            3554..3769
                     /locus_tag="LK889_001746"
     CDS             3554..3769
                     /locus_tag="LK889_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095981.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            3959..4213
                     /locus_tag="LK889_001747"
     CDS             3959..4213
                     /locus_tag="LK889_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095982.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            4392..5441
                     /locus_tag="LK889_001748"
     CDS             4392..5441
                     /locus_tag="LK889_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK5095983.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            5618..5848
                     /locus_tag="LK889_001749"
     CDS             5618..5848
                     /locus_tag="LK889_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095984.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            5851..6108
                     /locus_tag="LK889_001750"
     CDS             5851..6108
                     /locus_tag="LK889_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095985.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            6325..7200
                     /locus_tag="LK889_001751"
     CDS             6325..7200
                     /locus_tag="LK889_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095986.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            7210..8004
                     /locus_tag="LK889_001752"
     CDS             7210..8004
                     /locus_tag="LK889_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095987.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            8033..9151
                     /locus_tag="LK889_001753"
     CDS             8033..9151
                     /locus_tag="LK889_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095988.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            9184..9504
                     /locus_tag="LK889_001754"
     CDS             9184..9504
                     /locus_tag="LK889_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095989.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            9759..10448
                     /locus_tag="LK889_001755"
     CDS             9759..10448
                     /locus_tag="LK889_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HBK5095990.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            10485..10652
                     /locus_tag="LK889_001756"
     CDS             10485..10652
                     /locus_tag="LK889_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095991.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            10672..12300
                     /locus_tag="LK889_001757"
     CDS             10672..12300
                     /locus_tag="LK889_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095992.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            12351..12722
                     /locus_tag="LK889_001758"
     CDS             12351..12722
                     /locus_tag="LK889_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095993.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            12807..13007
                     /locus_tag="LK889_001759"
     CDS             12807..13007
                     /locus_tag="LK889_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095994.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            13141..14367
                     /locus_tag="LK889_001760"
     CDS             13141..14367
                     /locus_tag="LK889_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095995.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            15151..16475
                     /locus_tag="LK889_001761"
                     /pseudo
     CDS             15151..16475
                     /locus_tag="LK889_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            16468..16815
                     /locus_tag="LK889_001762"
     CDS             16468..16815
                     /locus_tag="LK889_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5095996.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            17130..17987
                     /locus_tag="LK889_001763"
     CDS             17130..17987
                     /locus_tag="LK889_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK5095997.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            18007..18567
                     /locus_tag="LK889_001764"
     CDS             18007..18567
                     /locus_tag="LK889_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBK5095998.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            19456..20223
                     /locus_tag="LK889_001765"
     CDS             19456..20223
                     /locus_tag="LK889_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBK5095999.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            20249..20794
                     /locus_tag="LK889_001766"
     CDS             20249..20794
                     /locus_tag="LK889_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096000.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            20795..21361
                     /locus_tag="LK889_001767"
     CDS             20795..21361
                     /locus_tag="LK889_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096001.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            21376..21624
                     /locus_tag="LK889_001768"
     CDS             21376..21624
                     /locus_tag="LK889_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096002.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            21644..21883
                     /locus_tag="LK889_001769"
     CDS             21644..21883
                     /locus_tag="LK889_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096003.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            22254..22535
                     /locus_tag="LK889_001770"
     CDS             22254..22535
                     /locus_tag="LK889_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBK5096004.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            22525..22866
                     /locus_tag="LK889_001771"
     CDS             22525..22866
                     /locus_tag="LK889_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5096005.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         8929 a         2994 c         4707 g         6732 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttctg atggacaaaa aattgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggcttag
      661 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
      721 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      781 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aattttctgt ttcaaaaaaa
      841 gagaaatttg tagatttacg aaaataaact atcgaaagta gtatgattat gatcttaaaa
      901 tatcatctta agagaagaat tacgattgtt tataggattt acaaaatgaa taagtttgaa
      961 gagtgatttg gtcgcagact agatcactct ttttgtgtaa aaaaacttta cacttatttt
     1021 tgagtgtgtt aagatttttt atatagagtg acggtttctt gtcagagtgg atgataacca
     1081 tcaatattgg aggaataata agtggaaaac aataatgata ttagatattt tagcattggg
     1141 gaaagagtta caacagtaag tgaattatca aaggattata gagataaaga aggaacgatt
     1201 atttctctta aaaagaatcg aagaggtgtt tggcaatatg ttgttgattt aggggatggc
     1261 gttacagttg agtgtaaaga tagtcaactg atcagaaatg atggcagtca ttccattgaa
     1321 aaagcaattc ttgtattccc aaaagtatca agcaagatta tgaatgactg gttgcttgga
     1381 aaatttggtg agtacaataa aaaagaaatc gcagaagtga tcggcaaatc ttatccaaca
     1441 gtattagggt atctaaaaaa gataacgaac aaagaagcga aagttgagga tgttttaaca
     1501 gtaagagaac ttcagtcatt aatcgaatat cggaataaac aaataacaaa tcctgagtac
     1561 cttaaaaaag aagatggtct attgaaacaa ttggaacagg taaataaaat ccggaaaatg
     1621 caaaaggtag ctcctttaag cctagataac tttaaaaaaa tctatttgaa agagatcgaa
     1681 aaagactaat gtggagtgtc actttaccgt ttcaacctgt atctgcacca agaccaagtg
     1741 caagaaggaa tgttgctgat gcaactaatg acgtagcaga gaaaaggatt tcaacctatt
     1801 atgataaaaa gtatctcgat tatttgaaaa aaataaaaag ctttatcgaa gatcaaggtt
     1861 tgttagatga tgaattttac tcgatcgtaa atgatccgat gggtgcaatt atggaagtgg
     1921 acttttacta tcagatacca aaaagctata aaaaagtcta taacattatg aaaacgacag
     1981 cgccagattt agataattta gtaaaaagtg ctatggatgg tattttcaac atctcagcta
     2041 tacgagatag ccatatcaca gggttaattg cttttaaata caatgtggca aacgaaggaa
     2101 aaacagttgt gaggatcaag cgatattcag aatttgagtg ggatacttct gaaggattga
     2161 acggagatat ttgggaagcc acatttgatt tcaaaccagt agcaacgcca agaccaaagt
     2221 ctaaatttag aggaaatggg attatcactt actatccaaa agagtataac gactatttgg
     2281 aaaatatcaa gaatacgctt aaagagaaag atttggtaaa tgatcaactg aaaaaggtaa
     2341 tgaatgctcc tatgggggtt atagctcaga tagattattt ttaccaagtg agaaaagata
     2401 agaaaaagct tgattcactg atgcgtactt cacagccgga tattgataac ttagtcaaag
     2461 gaacattgga cggaattttt aacaaggaaa ttggcatcaa agatagcagg gtggtaggtt
     2521 taatagcttt taaatacaat acttttgaaa agtcgaaaac aaacgtgcga ctacaggaga
     2581 tgggctgatg aataataacg aaaaaattct tcatgtactt gatagctttg aaacaattca
     2641 agaggaacta aagaaatatc gagatgtttt ggaacaaaga tatgattttg tcaatcagca
     2701 aaagagtaat cacatggatt tcattctcaa tatgaatgac ttgaagaaga aattagttga
     2761 acgaaaagaa caagaaaagt tgatcaaagc ttactttgaa ctcggtgaaa aagaagttaa
     2821 aaaggcaatg gaactgaatg aagatcgcag ggttttagat cagctgttag aacaattatt
     2881 ggtgatgttt caaaaaggac gaatagatga ggatttaatt gaagaagggt tgagaaagta
     2941 tcctgcaaac tcaggtatcg gaatagtgct gaaagcgatt gatgaagaag aaattgaaga
     3001 tttcataccg gcagaggatt ttgaatcggc aatggaatat ataaaatatt acagccaagg
     3061 gatcactgct tttagagagt ttgatcctga agatgttatt catgacttga acaacctaaa
     3121 agaatggtgt gaaggttatg aagttgatga ttccggtttg gactacttga tcagtattat
     3181 ggaaatcgaa gaagagatgc cggataagcc tgatccaaca gatattttag aattaatcca
     3241 tgatgctaga aatccaattg catacatttc tagaggatat actgtattgg aatactataa
     3301 gccttatata tcagcgatga accatttaag acgtgtatta agggaaaaac gtgaataccg
     3361 ctcagtgtta aatgcatcga atagattaga aaaagctgtt tcagagctag atgcttatta
     3421 tcgtgaacat tacctgcaag caggtggtat gccaagaaat acgaaagcaa atatcagtag
     3481 gtatataaaa gaagctgagt aggcggagtt agcccataaa atggcaaaag gtaacctgaa
     3541 agaggcgttt tgaatgaaaa gggatgtttt tgaattgtac gtattagaac gtctagaaga
     3601 cggcatgatg gcacaagaga taattgatga aattgatgat atgatcagca gatttggtca
     3661 attggaattg aatgatggaa acaaaaattc ggtagtgcta caacaatttt ggttaaataa
     3721 tttttcgtat aaggatttta tcaaagaaaa ggcaaataga ttgtactgac taaattcacg
     3781 aggatcagtg atgagagctt attgctaaga gcagttaggc tcttttttgt ttgtgttaaa
     3841 ttattttatt ttacaataca catttataca caaaaaatgt tgacggatag tacacaaatg
     3901 tggtaggata tatttgtaag caagttagat taacaagtta aaggaaggaa gagcagttat
     3961 gaaaaaagaa gaactaatag atatgtttca aattgttgaa cgagcaaata atatgggaat
     4021 tatgtttttt gatcgtattt ctttaaagat ggatttaagc gttgcccacc aagaatttaa
     4081 tctacgatta aaagctttat tgatttctga tgatgttaat ttcgctcatg atgttgtagg
     4141 tatacagaac catatagata gagaaaacaa acgtatgggt gatggattct taccaagata
     4201 ttcaagttta tgatttggcg aaagattcaa gagtatgtgt cattattgat aggtactctt
     4261 gaattggaaa ggaggaatga atttaagaca acaacgatta tgatcttaaa acaatcgttt
     4321 atgtaattgg caatcttacg aataattttc atgcaataat gacaataaaa aatgaaaagg
     4381 ggtaaaatca aatgaaaaaa tttttagtag gaattctatt cacaatgggg ctagtgctat
     4441 tatcaccttc agtttcagca gaaagctcta tttccatgta tcgtatgtat aatcccaata
     4501 gcggagagca tctatacact agaagcgttg gagaaagaga caatctgaaa cgttatggat
     4561 ggcattacga aggtgtcgcg tggcaagctc caacaagtgg ttccccagtt tatcgcttgt
     4621 acaacagata taacggtgaa catttttaca ccgttgatgc cggagaaaga gataagattt
     4681 cgcacacagg atggacgtac gaaggtgttg cgttctattc ctacaacgga gcaaatagag
     4741 ttcctgtgac acgcttgtac aacaaatacg tgaattggca ccattacacg ttagatcaaa
     4801 atgaaaagaa cgtaattagt aaacaaggtt ggaagattga aggtacgggt tggtatgcaa
     4861 tgccaaacag cgcaccaaat cctacacctg cacctgctcc aagtaatgaa aaagtgttaa
     4921 atgttcctgt catttggcaa ggtaatacga tgttgtgtga aggtgcttcc ctgttagagg
     4981 gattgcacta caaaggagta gcgacaaatc aaaacttaac cagttttgta aactatatgc
     5041 caagagctaa tgacaataat ccatatcatg gctattctgg cgaatggcgc tacaacgtaa
     5101 atggtacgta tcaaggcatg atgcctgatc cagtagttaa atgggcaaaa aatgttggtg
     5161 gtaatgccgt taatatcact ggttcaggaa cgaatgggat caagaatgaa attcgcaatg
     5221 gtaatccggt tgtaacttgg attacttata actatgcttc acctcaattc aaacaaatgc
     5281 cttggggacg tgcagtctgg aatggacatg tggttctcgt ggatggcttc aaaaatggtg
     5341 cataccatgt tgttgaccct gtatttggcg caaaatggat caatgcaagc acgtatgaga
     5401 gagcatttaa tgtgactgga atggcggttg ctgtacgtta attgatagaa ttaataaata
     5461 aatgaaataa gagcttaatt actgaaatgg taaatgggct cttatttttt agaaaaaaac
     5521 tgttttttgt aatacgaatt tataacaaaa aacgttgaat gtagtacaca aaagtggtat
     5581 attaaaaccg tagagattat gcgaagaggt gattttgatg aaccaaagaa gaagaaaaag
     5641 gctagaagaa ataagcgagc gactatccga gttaaaggat gagttagaag taattcaaga
     5701 agaagaggaa gagtacaaag aaaatatacc tgaaaatcta cagggatcag aaagatatga
     5761 aaaagcagaa gaatccgttg atcaacttga agaagctgtt gatggtttat ccgacatatt
     5821 agaattgatt gaagatatag ctagctagag atgattaatg aagatttcag gagtgagcaa
     5881 aaaatgattg atatttcact gatagagaag ctactttttt caaataagta cacagccaaa
     5941 gagttggcta caagatggca aatatccgaa tcagctatta aagcctatcg tcaaaataag
     6001 ggaagcgtag gatggcgtga ttggaaagga atcagcattg gaaaagcaga agacataatg
     6061 aaacgtgaag gatttcaagc tgacaatgat tctaaaaaag ttgaatagtt taatagtcgc
     6121 caagaaaagt accaggacga ggggaaaggt agattacaaa ggaaaacact tttaaataaa
     6181 cttcgcaaaa gcatcaggtt atgagtggct agggctatac gaatgtgatg aaaacaaagg
     6241 gctttaatgt caaagaaaac gcaatagggg gggaacaaaa tttttaaaaa agatatttat
     6301 gtttggttta atggaaaaaa atatatgact actaacaaaa ggtggtcaga gctagtacaa
     6361 aatacagtta aaaaagtcat gccagatgga tatcatttca actatataca atgctcacaa
     6421 acaactgaaa gtatttattt ttcaatatta aatgagaata gactctatta tttcagggta
     6481 agttcacacc ataggaaaaa gagagaagat aatgttacga catttttatc gggaaaatac
     6541 aatggcttca aagagttaag tgaagccatt ggtgagtatc taaaaagtaa tagatttgtc
     6601 agaatttctg acaaacatat attgatcttg ttactttatt tgaataatag cttcaaattg
     6661 ttttggagag acgacacttt ctactatatt gagaacggca tggatattta ttctggtatc
     6721 aacggcatat taaaacatta tattaaggcg ttacttcgat atggggttct ttatcaagaa
     6781 gatttaagta accaaattaa gattagtaat tctggaaagt cattactggc aaaatataaa
     6841 aaattgcttt ctcctatcta caaaggaata gatatggaag ctgtaaattc tgaaacttta
     6901 tttaatttag ttgttgatga aaagtttgcg aatgaatttt ttaaaagtaa aaaaattgaa
     6961 agactaaata acctgtttcc tgaaaatgca ataaaagctt atgtagataa taataaatca
     7021 cgattataca actggtttat tcccaaacgg ttgcgaggaa aaatcaaaat tggcgatcaa
     7081 gtgaaagtaa ttaatctaaa tcatactaag atcgttaccg taacggagtt gtatatagct
     7141 gaaaaagaag atattacaaa aatgaaacca gttattaaaa tactagataa caaagagtga
     7201 gtggaatata tggtagcaaa aaagaatact ctaaaagaag cttctgaaaa atatcaagat
     7261 atgaaaagac gaggattaat accaaaggac aaaaaaggta tatggagaga atttgttaag
     7321 gaagtcagta gtgatcgaag tggcggtgac tttgttggac gatatacagc aaaaggttat
     7381 agcaggcgct taaggatcac tgttgaatat ggaacgaatg aatcagctac agagctgaag
     7441 caaaggtact tagatacacg aagagaagct tcaaaaaaat atgatgattt caagagcaga
     7501 ggtctaattc ctaagaagaa aaaggggatt tggcgtgagt ttttcaaaga agaagatagc
     7561 aatcggaata agggagactt cattggacga tacacagcaa aaggctacac taagaattta
     7621 gggatcaagg ttgaatacgg aactggtgaa agttcatggg aattgagaaa tcgctattta
     7681 aatacattgt tagagaaata taaagcagaa ttattggagt tgttcgatgt taatgatttt
     7741 atcgagatgg ctgaaaagta tggtcatgaa gaaggtttta tgatcaatga cattatggtt
     7801 attgataatt ttacggattt attagcactt gcgaaaaaag caagggattt aagagaaacg
     7861 cagatgtatc tattcagaaa taaaaacgga agaaaccgga gaactgaatc gagtgagatt
     7921 gaagattact atgatgcctt tacacacgat ctgggagcct ttgtagacga gataagtggt
     7981 gtaatgaaca aatatagggt ctagaataaa aaagccggag atgagcagat aaatgggaaa
     8041 caatagcata tacacacctg aaatgcagaa taaaattaaa agacttgtcg gattagggtg
     8101 gatgacaaga ggaaaattag attcttttag tacgatcaaa tatcttttga tggacattta
     8161 ccgtcagaac aagcaaacag gaatacattt tttgggctat gataagccgg aagatgcacc
     8221 tttctttgtt ccaagttcag cctatagagc cagtacctcc ggtagcatgt ataacttgga
     8281 tttgtttgag gaaggagcaa agatgatcga atatgatttc aacgaagcct attcgaggat
     8341 catgagaaat tatcctttgc cgtcaaacac ttatttgaaa gcaaaatata atacagatca
     8401 agctattttg agagcgataa atagaccaaa agtaagaccg tatgaaaatt taaaaacgta
     8461 tatgttcttt ttggttaatt ttgaaggaac agccaaagaa ggaacatatg ctaatccaaa
     8521 atcacagtcg atgtttgcgg attatggcga aaatcccaaa acagctaatg agcctatttg
     8581 gttatcggag attgagctaa cacttatcct tgattattat gatattaaag agatggaagt
     8641 cctagatagc cacacatttt tgacgaaaaa aggattactc aaagaatatt ttgaaagaat
     8701 cgagccatta aaaactgatc ctgatacgtt gaagctttat aaaaatctta gaacgaaagt
     8761 atttggtgct atcggacaaa aatcattaag agaaggagaa acaagcacct tcgataaata
     8821 cagtatgtat aatcgagcgt attcctccat ggtagcaggc gtatttagag atcgtatggt
     8881 tagatatgaa cagaaatacg ttcatagcgc atatgggcta ttaaaaattc gtactgatgg
     8941 attgtatttc agatccgaag taccagagtt tgaagagctg tataaacttg gagtagttaa
     9001 gaaaaaagtt catacaatat ccaaagaaga agtcgaaaaa gctttagaat ttaaaaacaa
     9061 aggatttgaa aataagggct cctttgggaa atctaaagat gaagttaagg gtgttgtgat
     9121 agacgatgat ttcgagtttg attttgacta aatactcttt ggaaaagatg gtaggaggta
     9181 aatatgaaga agaaaattgt ggaaggaaat gtaggtgtta ttattctaaa agattcaact
     9241 gactatgcta aatcaatggc actaaaagac gaaaaagttg cacgcatgga taaagattta
     9301 gttgaatttc tgatggatga tacagaaaga ttaaaagggg atcagttttt gagtttaatg
     9361 aaagacaaag gcttaaacac atatggttta tttgattata agaaattagc tatagagtgg
     9421 gtaaaagaag gagaaatatt ttttattgaa gaatgggaag gtactgagaa cttagttctt
     9481 catcagtatc ttttgaaagc ttaatctaag taaaacagtt ctgactagtt atgcttgtca
     9541 gaatttcaaa ggaagttttc cataaccatc atcattcaaa aataaaggat ttgaaaataa
     9601 gggttccttt gggaaatcta aagatgaagt taagggtgtt gtgatagacg atgatttcaa
     9661 gtttgatttt gactagattt ttttgcaatg tttaagagta aacaaggtaa gaactaagat
     9721 catgaagatt gagagtaaag aagatagagg acgaatgtat gagaacacac tatatttatt
     9781 tagaagataa aattgtagat gaaattccgg caaaaagtcg agaacaagct agaaaattat
     9841 ttattgaaaa atacaaagag atcaatggag atagtgaagt tccaaaagtg gtcgtaacag
     9901 ttagaaaaag gcaaacaaaa aagagaagta cgtttcatgt cagccctgat atatataatc
     9961 ttatagagcg acatagaagt gaagataata tttctatctc tcaatatatt gctacagtta
    10021 ttgatgaatt gatttcagga aagtatgact atgcttttga ttttgagggt gaaaaggatt
    10081 acaaaagtgt tgctgtcttt caatctgatt ttgagaagct aaaaaactat gctggcagga
    10141 gaaatgttaa ttttgcaaga ctactagaag agttctactc cgaagagttg ggagtaggaa
    10201 atgaatacaa gagagaaact tatgcgaaat tgtctcataa gttatcccaa gcgcttaaag
    10261 aagtgacaga agttaaagcg gaactagatg ctaaacttga agaagtgaaa aaatatcaaa
    10321 cagagaggaa gaacaacatg aagaatcaag tggaatttga cgaacagaaa gaatttgaaa
    10381 aagcaagaaa acgctataac aaaattatga atgaacttag ctatgaacac ttaacagttc
    10441 atgaataatt gagtgagggt acagaaaaat atacgatcgc agatatggtt aatgaagtgg
    10501 aaactctttt agcttctttc tttgaaaatg gtcacgtaaa cgatgaatgg cgaaaagaag
    10561 aacctaaaag ggttaacgct tatattgctc gattaaaaag gtttattgat aaatataagg
    10621 agaatagtga caaattttct aaagataaat aaggagagag tgtgattaca tttgccagaa
    10681 atcgaaggaa tattttcgcc aaaaataggt aaaaaacagt tagatttcag cagttttccg
    10741 agcgtaaata gagattataa tctaggtgat gaggtacaga tactaaatat tgatttcaaa
    10801 gaactagact tcgctgttgc tgtttccaac aaacccaagg cattggaacg agtgaaaaaa
    10861 atataccaag agaatgatca aaatatcaaa aatatgatca aaaagtatag acgagataat
    10921 tcaaaagttg aagacgtttt attcaacggt acggcaaaac atgatttgaa tgtttatatg
    10981 gcattaggag tggctttgta ctgtaattca attaatcaga cagatatttt ggttaatatc
    11041 gtgaggaata gctatcaaaa aatattaaaa ataagtagcg aaacaaaccc ttattatgca
    11101 atgaaatatc ttggggataa tttattaaat gataaaaaat tgatgcacaa cactgaagag
    11161 ctgttatcta atttggctgc gattaacttt ctattcaaaa caggttctga agattatttt
    11221 atagataacg tactaaaagg tgcttacgga acagcatttt atccgcttta caatatgaaa
    11281 cagcataaag ccgaacaaaa ggataacaaa cttgaaatta aaaagatgaa agaactatta
    11341 ggtataggat ttgtaaaacc caattcaaaa gtcactattg cttcgttagc aactactaac
    11401 aatgatggga aaatagtaaa tgggtttgac gattcagata tagatatatt gtcaaaatca
    11461 tatttttctt tgtatggaga ttttgcagat ttgatgggta ttgatagtta cttagtcaat
    11521 aattttgaga tcaagggtag cgagctgaat gatattgtgt atttcgtttt aatctctcta
    11581 tatgcaattg atcagaatac cgagtaccga caaattgact atttgctagt tttttatagt
    11641 ttgttatatc agttcgtttt aactcggttg tttaaaaatg ctaaggaaga attgatcctg
    11701 aattctgatt atgaaatagc aaaacaaatt gcaagtatta gaaaagaaaa caaatctgaa
    11761 gtaatcaaat taagaaaaga attaagtatt gcgagtaaag aaaacggtcg gttagaatct
    11821 ttagtcaaat cggcaaaaaa agaagaggaa cttttgaagc aagagattaa agaattaaaa
    11881 ggaatcagaa aagaacttga acagctgagg tttgaaaata aagtattaaa gcaattgcag
    11941 gagcaagaac atagacaatc attaaatggt aaaactgaag agccttcact gtctgaaatg
    12001 atagcttatc taaaacataa aaacattctt ttagtgggag gacatgtaaa ttttcaaaat
    12061 aaattaactg aactattacc aaatattaaa atgtttgatg taagcgaaaa taatggaaag
    12121 atacttaaaa atttgaatac gtgtgatgcg atttttctat atacagatta cttgaatcat
    12181 ggtctatatt atcgtgttat gagtgaaatt tctaataaag ataagatatt ttatttggat
    12241 aatcatgtga atatgaactt aacaattaaa cgtatttacg atcttttatc acttatataa
    12301 agagagaagt gataaatagg acacaaaatt tggaaagtag gtaagtcact atgagagaac
    12361 taactttatc ttctaaggaa ttagaaaaga aaactgcttt attaaacgaa atatttagtg
    12421 ctgattgtat aatccaaagt ttaaaagaaa gttcattttc aaaactaaca gatcaggaaa
    12481 aaatttctta tatgctaagt ttagataatc taggaactaa atatgatgaa tcagcaagta
    12541 gcgataagct tttgattgaa ttagtatcaa gtcttagtag ttttggaaaa atgaaaagat
    12601 ttaatgtgat attagaaatt tttgcttggt taatcccttc atttatttgg tttttttata
    12661 ttttcatagt tgaatctgag ttaatagcca aggtctctct tggactgtta attccgatat
    12721 aaataggttc agcaatttac tttaataact tgtatacacg tattcaaata aaaaagcagt
    12781 tattacatga aggattaagt ggtaatatgt taaaaagaat tgcatgggat aaagataata
    12841 aaacagatat tgagcttaaa gaatataaat acaattattt ctcgagatgt atagatagaa
    12901 agttactgtt taaaaaagaa ggattagaat ttagttttga tgaagatgaa tttgaaaaat
    12961 tagtgatgga ctggcgtaag aatttactgg atacggtaca attctaaaag caattgaggt
    13021 cgaaattccg atacttgaat catgatctat attatcgtgt tatgagtgaa atttctaata
    13081 aagataagat atttcatttg gacagtcatg tgaatatgag cttaacaatt aaacgtattt
    13141 atgatctttt atatgtttat aaacattaat gaggtgctaa aattgacaac aataactatg
    13201 acaaaaaaaa tagaagtata tgttgatgga tcacaagaag aaagggattc cttttttgat
    13261 tttttttgga aaaatagtag agaatatgta aaagcgttga atctggtata taaacagcta
    13321 atgtttgaag agttgataaa agatgcaatt aaagaaatgg atcaaggtta tgctgagaga
    13381 gaagaaagta ttcaaaatcg gattgaaaag ttagcatata ctgatccaat agatcaaaaa
    13441 aaacttgata aagcaaaagc tgaattatct aaattgagaa atgaaaaaaa taaagaagcg
    13501 agagaaatat taacgcaagc gatagggttg aagagtcaaa cgagaacacg agatgtagca
    13561 aaaaaattag actttgactt tggagatttt gttgattccg caaatatgaa agctagtaaa
    13621 gattttggca atgattttat cggaattgtg accggacaaa gaacgcctag aatatataaa
    13681 aatagaaaca aatatggatt actacctttt agaggtcgag atattaaaat ccataaagat
    13741 gataagtttt atatttattt acctaaaggc tttaggttta aagtgaattt aggtagcaag
    13801 ccaagaaaag caagagatgt tgcttcagtt ttacaacaca ttatggatgg cgattacaaa
    13861 ttaaatcaaa gtaatcttat ttttgatgga agaaaattat tttttaatct aacaatttct
    13921 tttgaaaaaa gctataatga aatggaattc aagaaaggta atgaattgat cgtaaacttt
    13981 gcagatttgt gtagcggtga atgtttaatt aatgataaat ctgtaatgtc ttttggtgat
    14041 agccaatata taagaaactt caaaagtaaa attttagcaa tgaaatcaaa agaacaaagc
    14101 cgaggagtct tttctaaggg cggtcatggt cgaaaaagaa aattagatca agatcgatgg
    14161 gaagcaataa agaaaagaga aagtaatttt gttaaaactt ataataatca atgttctgca
    14221 aaaatcgtta aaactgctat tcgcaataaa gctcaaatta ttcgtatcat tgaaccagat
    14281 aaggcttatg gcgaatgggc ttactatcaa ttgaaagaac aactagtaaa caaaggaaaa
    14341 agatatggga tagaagtcag aagttagtaa attaaacaat cgttgatttg gcaacgaaat
    14401 agtgagggaa aatccactca ctgaagggta aactccgtca agttgtgtga ccagacggaa
    14461 ggacgaatgt tgttgaggtt ttagaaccat ataaaacagc atgtttctca aatgagtact
    14521 ttaaattaat tgatggcgtt cctgctttag aaccatataa aacaacatgg atttaaaaca
    14581 agaagtaagt cagttctcct tttactttag taaggaaaaa taaagtattc tcaataaaac
    14641 ttaaaataag caaacctttg ttttaatagc taatcgaagt aaaacccaaa gccatatgaa
    14701 cccctgaatc agcgaaagtt tatgatgatt tggattacta atagtttagg aattagatgg
    14761 taaatctaaa ttaaacaatc gttgatttgg caacgaaata gtgagggaaa atccactcac
    14821 tgaagggtaa actccgtcaa gttatgtgac cagacggaag gacgaatgtt gttgaggttt
    14881 tagaaccata taaaacagca tagttctcaa attgtttaaa atgattgtca agttaaaaaa
    14941 atctgggatc atataaaata gtatagttct caaatatgat tagaaaccct attaaagagc
    15001 aatattaagc caacttactt aaatcgttta attaagaaca ataatctctt ttagtcagac
    15061 ttttagtctg acttttttgt atttccaaaa tttattcttt acaaaaacga acatacgttt
    15121 gtataattgc ttgtgaatgg gggaataaaa atgtttgatt acagtagaga accggtgaat
    15181 gactattttt taatcgacat gaagtctttc tatgctagcg tggaatgtat tgaacgaaat
    15241 ttagacccaa tgactacacc gttaattgtc atgagtagat cggacaacac tggttcaggt
    15301 ctgactctag cttcttcacc tactgcaaag aaaaaatatc atattactaa tgttagtaga
    15361 gctactgatt taccttatcc attaccaaaa gaacttcata ttgttccacc acgaatgaac
    15421 ttatatataa aaaaaatatg gaagttaatc agatttttag aaagtatgtc gcagatgaag
    15481 atttattgat ttattcgatt gatgaatcaa ttttaaaagt gacaaaatcc ttaaatttat
    15541 ttactagttc aggaacaaga agcgagagaa gaagagaatt agctaaaata atcaaagatg
    15601 agattcagaa agaaacaggt ctaatagcaa cagtcggaat aggagataac ccattattag
    15661 ctaaacttgc actggacaat gaagcgaaga gtagtaaaga atttatagct gagtggactt
    15721 atgagactgt acctgaaaaa atatggaaaa taaacgagat gactgacttt tggggcattg
    15781 gatcacgcat gaaaaaaaga ttgaatcata tgggaattat gagtatagaa gatttagcaa
    15841 attggaatcc ctacacaatt aaaagtcagt taggtgtaat cggactccag ttatatttcc
    15901 ataccaatgg aattgatcgt acggatattg ctattccacc agaaccaacc aaagaaaagt
    15961 cttatgggaa ttcacaagtt ctaccaaaag attatacgag gagagaagaa gtagaactag
    16021 tagtcaagga gatggcagag caagtagcca ttcgaattag acaacacaat tgcaaaactg
    16081 gttgtgtacg cttagcaatt ggtacgtcaa ttttggaaga aaaatcaggt ttctcgcacc
    16141 agatgaaaat accaattaca gataatacga aagaattaca aaattattgt ctttttttat
    16201 tcaataagtt ttatgagggg caggaagtac gacatatagg agtaacttat agcagattag
    16261 tatacacaga ttccctacaa ttagatttat ttagtgatcc aagaaaaaag attgctgaag
    16321 aaaatttgga taggattatt gataagatta gacaaaaata tggatttaca gctattatac
    16381 atgcttcatc tatgttagaa ggtgcaagaa gtattacgag aagcacttta ataggaggac
    16441 acgcaggagg attaggaggt ttgcataatg attgaatata acgatagagg acgtttaaaa
    16501 tggtcgggat tttatttgtc tgatcatact gaaagaatca atgcagacaa tgtacaacgt
    16561 gctaatcata atttggcaaa agaacaaatg acgactgaag agattgcgaa aatactgaga
    16621 actgctgtca taaaaaatag acccgtctgc attcaaaaag aggaacgaaa cgctgaaggt
    16681 tactatccag atgatgtagt aggaaaaatt attggatatg acgagcttgg tgtatttgtg
    16741 gaagaagaaa aagttcacta tgatgaaatt aggaatgttt ctttctcgga attattaaaa
    16801 tggagtcaga tatgaacatt attaaaaaat acgaattcgg ttatattacg tatgaacagc
    16861 taatagagga attatgggga tatggtcaac aattaattaa tgaagtgggg attgattgct
    16921 tttgtttcta tattgaatcc ggagcaggat accataagaa cagatactat atatttccat
    16981 atccaccaaa atagtctctt agatattttt aaattttctc caaatacttt caattattgt
    17041 gaaatatttc cctttaatta atcaaatttt ttataaaagt aatatataaa ataaattgct
    17101 tgaagtatta tatttagata tcataattta tggaagaaaa attaattaaa aaggatgaga
    17161 ttataatggg gttactggtt ccgagagcta atacttatac tataccttta acgaaacgcc
    17221 aattaattgc taaaagatat caaaggatta ctagagccat caatagagag ttttggaatt
    17281 ctgaaagtga tactgctcac agtttatacg taggttctta tgggagagga acggcaatta
    17341 gtactagtga tatagatata atagttgaat taccgatggc agaatttgac agattcaaaa
    17401 attatttatc aaatggcccg tctaagttat tacaagttat aaaaaacgca tttcaggaaa
    17461 tactaccaaa tagtgatatt agagctgatg gacaagttgt taaaataaat ttccatgatg
    17521 gaatcaaatt tgaaatagtt ccagctttta atgaaaaaga ttattggggt gagtcaaaag
    17581 ggtttattta tccagattca aacatgggag ggaactggaa agctacaaat cccaaaaaag
    17641 agcaagaagc catgaaatta aagaatacaa aaagtaataa tttattatat gctacgtgta
    17701 aacattttcg acatgtacga gatacagaat ttacgagtta tcatctttca ggaatagtaa
    17761 ttgacagttt cgtatatgag gctatgggaa attggaaatt cgttgaaaat aacagtgggg
    17821 gtcaaaacat tagttctgta tcttatgaga cagctttatt agaatactat aactcacata
    17881 aagttatggg cggtttaaat ttatactctc ctggaagcaa tcaatttgtt aattctgata
    17941 gtagtatcat atgtttagaa aaagtattaa aaaaaatagc tttataaagt tttatggagg
    18001 aacaacatgg gagacgaaaa aaagcgcttg gaagttcaat tacgtgaagc atatggacga
    18061 gtaacgtata cttacaccac tcatttaaag caagccgata atattatttc tgacaataaa
    18121 attttaaggt gtcttcaaat tggtttgtca gcccttacta ctggaggggt acttagtacc
    18181 attattactg ataatagtga gttaaccttt gttgctggat tatgttcaac aatttctctt
    18241 ggattaaact tatactttaa gaattttgat cttgctgaag aagctaaaaa tcatcaaatt
    18301 gttgccgata aattatggtt gatcagagaa gaatacgtaa gcttactaac agatttaaat
    18361 cgtttaagtg ttgaagatat tgtgaaaaaa agagatcaat taatgaacga aactcatgaa
    18421 gcatataaaa atgcccctaa aacaaatagt aaaagctatg aatcagctca gaaagcatta
    18481 aaatctgaag aagagcaatt ttttacagaa gaggaattgg ataagatgtt accagagcat
    18541 cttagaaaaa agagttctaa atattgaact ctttttttat gcttaaaatt ctcttgtaat
    18601 ttttctattc atctatattt aagaagaaat atgatgatag aaacatctcc taaagccacc
    18661 gcaagccgtt ctaagcatag cttagcccga cattgctaga tatgctcatc tcaccatgaa
    18721 cgtttcctaa agccaccgca agccgtccta gccattgcct agtccgacat cgctagatta
    18781 gtgcatctcg ccataaaagt tttctaaagc caccgcaagc cgttctaagc atagcttagc
    18841 ccgacattgc tagatatgct catctcacca tgaacgtttc ctaaagccac cgcaaaccgt
    18901 cctagccatc gcctagtccg attttgcttg atcggttcca tttctgttta tttagtgggt
    18961 caacaccgta atcttaaata gccatcgcct atttaagatt actaggtctg ctttgaaggt
    19021 aaaaagtctt tcattcttca agacttttag gctttcaagc tacgcctgaa agcattgaaa
    19081 ttcatcgctc tttatttggt tttgccattt ataattcctt acgcccatta tctttttcgc
    19141 ctgtcatacc ttgtcagaag tgtttagagc ttatctcatc tttggattaa tacaagtggc
    19201 gtaaattttt ttctaaacgc aagcgttacg aaaataattt acttaaacac ttgtattaat
    19261 ccggcagata agaacgcatt ctaatcactc ccgacaaggc aagacaggcg aaaaagataa
    19321 ataaatgaac gaaaggaaga aacataaaat gcaaaaccaa atagcgatga agaaaaaacc
    19381 acctccctct aaaacaatcc aaacaaaaaa gaattttgaa aaaccaaaaa tcaaaaacag
    19441 aaagaagcgt tcaacatgag agatagaatt ttagaagtta atcaagaaat ggtaaacaag
    19501 ttaacaaata tcagtgggaa ctatgaaaag tacgatggta ctttacttga taactatatt
    19561 tttcacgata cacaaaatat cagaattggc agaagtaaac cgagagcgtt cattctcatc
    19621 aaagagaaat atttgaatga atggaatagc acctatgaaa tggttatgac tgataacttt
    19681 gaaaaggtat tggatttcag aaatgctttg ttgggttctg atatgggaaa tagcagtctt
    19741 gaatctattg aagttgaaaa caagaccgag aatgaaggtg aaggattgat taagaatgtc
    19801 ttgaaaatcg aacaaaaata tatcagtgaa gatagaagtt tttatagagt cacaagctcg
    19861 gttggtctag caaactttgc tactaactta attggaaaca atgctagtga atctttgcta
    19921 gtgatgtgtc tgaatacaaa gaatgaggtc attgcttata gcgaagtttt caaaggtgga
    19981 attaatcaat cagtagcgat accaagagag atttttcaac tagctttgct aaacaattca
    20041 gcaaaaatcg cagtatcaca taatcatccg agcggatatg aatttccaag tgaaaatgac
    20101 aaaagtttta ctacgaattt gaaaaacgca ggaaaaacaa ttggtattga attgttagac
    20161 catatcatcg taacaagtaa aaacacaagc tattactcat ttcaagaaaa tggttcttta
    20221 taaaaattag agaaaagagg aaaacaagat gggcgaaaga acacaagcgt taattacagt
    20281 caaatatggt aacagcaaaa cgaaagcaag cgtgtcgatt cattatcagt gggggtatgg
    20341 tcgatatatg ctgatggacg tattgaatca aggaatttat ttaatcaata aagcatacga
    20401 cttacgagat aaatcaagag aagaattagc aaaaggaatc tttgatagaa gtacaggagt
    20461 tatcaatttt gttacctatg agtctggagt gagtgcttat gatgaaacag ggtttggtgg
    20521 gcgctatgaa ccttatcaag aaacgattga agatgtgatg aagttttcag ataacaatga
    20581 tggctatgct catttagaga tggtgttcga tgaaaatgga tttagtctag atgataaaac
    20641 cagattgcga ttgtttgatt ttgagggtca acagatagga ctaaacaagt tttgtgaaag
    20701 cggaaaagag tttgctacga aagattttat taagggatac agattattgc tagaaaatta
    20761 tggaataaac ctagaagaag agagcgtgaa ataagtgttt gaaaaaagaa aagaccagtt
    20821 ccctaattta gaagcgaaaa gtcaaaaagt gattgatggt gaaatgattg aattattcca
    20881 aaaaagttat ggaaccatag aaactaccgc aagttgcaaa tactactttc aaggaaagag
    20941 aaacacgttt aaaggctttg ccgattctga tgaagaattg gcaaagaaaa tcattgattg
    21001 gcttgtcagc agtgaacacg tagcaaggga agataccgtt agaacaacga ttaatgggga
    21061 agtcctaaca ggtactagat gtgtttgttg taatcatgaa tttgtagaag atgaaggttg
    21121 ctaccgaatc aatgaatatg gtgattttat ttgccagagt tgccacgaga tagaatttcc
    21181 aaccgaaaga agttggtttg aatacatgct acaagattat gagatggaaa aagaaaatgg
    21241 tgaagtagtt ttttgtacca aagaggaatt gagtaagaga agcgatgatg aaattgaaga
    21301 ttggtgtgtg tggttagtag gggcagatga ttgcgttgca tattacacaa cagcagaata
    21361 ggagtgtgaa tcagcatgga aggaatggat tttctagatc atgaagattt ggttgatttt
    21421 ggttatacat ggaaaggaat ggtaggtatt agcagaagtc ttgctaatgc cttttacgaa
    21481 agaaattatg cagtatatgt tctttatgat gacgatacag aaagccttgt tgacgaggaa
    21541 tataaattgg atttagagaa tgttttatat ggaatagaaa aggaagattt agcaaaatat
    21601 attttttcat ggttgggtca ataagtgaaa ggaagataga aacatgacaa aatttaaaag
    21661 taagggtatg caatggtgtt cttattatta tgttggaaat gatgaagatt tattgagaaa
    21721 aacccaacga aaaggttatc cagataagat attgaaacaa ataggttatt acatgccaga
    21781 agagaggata gaaaaagaat tttgtgaggt tctttttgat agtgaacagc tattagtttt
    21841 gttaacccaa aataaattga tttgccaatt tgaaaataat taagatgagg tggtgaacca
    21901 tgaatgaaga taagatggta ttaagtgaat atgctagatt gagaactatt aattctttag
    21961 catctcattt aagttgttct ttcctaacaa aggtgctaaa attaatttac aaatgtgaag
    22021 attttgtaca agagaagcac aaagacaagc ctactgcgaa taggcttgtc tttttttcat
    22081 tcattacgat tccgtatgtg tgggttagcg acaatttgtt caattaccgt atgatatagc
    22141 atcttaattt gattagatta cttgcaatgt agtttattaa gagtataata taatcataaa
    22201 tgtggacaat ttgataaata atgagtgttt tatactcaaa aggagtggat gaaatgaagg
    22261 tcaaaaatcc aactgaaaaa agcagaatcc aagtcggaat agacaagaat ttaaaagaga
    22321 acgcagaaat gatattggaa gagctaggtt taaatcctac aacagcaata acaattcttt
    22381 acaaacaagt agtcgctaga ggtgagtttc cagtagagat aaagctaagt gaagaggaga
    22441 agcaaggaat cagattgcaa cagctaacta aggacatgcc agtagatgtt ctagatacag
    22501 atgaaaagtt agaagagtgg ttcgatgaag cctaatgaga tagtaacggt atatgttgca
    22561 tttacagata aaaatggtgg taaaagaaga cctattttgg tggttagtga taaagaagat
    22621 agagtagaat tctttggaat cacaagtcaa tatgagaaaa aatcagacca aataaagagg
    22681 gtgtattttc ctattgcaga atgggaaaaa gcaggactta aaaagcaatc atggattgac
    22741 gtagggtctt taaaggctat tcccaaaagc cgagagaaca tatcattcaa aaaggttggt
    22801 atgttatcag ctagtgatac gaaaaagcta aataggttta tcgagaaatt acaaagaaaa
    22861 gtctaacaag gcttttcttt tttgtttata tgtatattaa gatatacgtt tatataaacg
    22921 tatttatgta tatgaaaata tacgtaaata tatacgtggt tataaacatt aataaaacgt
    22981 taagaattgt taaataatga tatttagtaa tatacgaatt ataatatata ttttatataa
    23041 aatgtatgta aataaataaa atgtctgacg gatattgaat agcaataaat aacaataatt
    23101 cagacggata cggtgagtga agtagcttgc tacgaacgaa caggcagaat gaaacggcag
    23161 tttcattaga gcaagtcgct tgcgacgtag cgttaccgct gatgtaactg gcaagttaca
    23221 tagagagagt ttgcttgcaa acgaacgtta cctatttgaa tagaaataaa aaataacaga
    23281 ttagtgaatt ttgaatagga gccacaactc aaccaaccgc ttgcggtggg tgagtcccac
    23341 ggaggaaaag acgaacgaac gg
//
LOCUS       DAFBQW010000036        23052 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFBQW010000036 DAFBQW010000000
VERSION     DAFBQW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.037"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            78..2519
                     /locus_tag="LK889_001772"
     CDS             78..2519
                     /locus_tag="LK889_001772"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBK5096006.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2849..3553)
                     /locus_tag="LK889_001773"
     CDS             complement(2849..3553)
                     /locus_tag="LK889_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096007.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3740..3985)
                     /locus_tag="LK889_001774"
     CDS             complement(3740..3985)
                     /locus_tag="LK889_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096008.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4002..4853)
                     /locus_tag="LK889_001775"
     CDS             complement(4002..4853)
                     /locus_tag="LK889_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5096009.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5019..6023
                     /locus_tag="LK889_001776"
     CDS             5019..6023
                     /locus_tag="LK889_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBK5096010.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6264..6620)
                     /locus_tag="LK889_001777"
     CDS             complement(6264..6620)
                     /locus_tag="LK889_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5096011.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTIREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            6988..8175
                     /gene="tuf"
                     /locus_tag="LK889_001778"
     CDS             6988..8175
                     /gene="tuf"
                     /locus_tag="LK889_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK5096012.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8363..10954)
                     /gene="mprF"
                     /locus_tag="LK889_001779"
     CDS             complement(8363..10954)
                     /gene="mprF"
                     /locus_tag="LK889_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK5096013.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10980..11741)
                     /locus_tag="LK889_001780"
     CDS             complement(10980..11741)
                     /locus_tag="LK889_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5096014.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12019..12540
                     /locus_tag="LK889_001781"
     CDS             12019..12540
                     /locus_tag="LK889_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBK5096015.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12599..13459
                     /locus_tag="LK889_001782"
     CDS             12599..13459
                     /locus_tag="LK889_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBK5096016.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13644..15959
                     /locus_tag="LK889_001783"
     CDS             13644..15959
                     /locus_tag="LK889_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5096017.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16141..17982
                     /locus_tag="LK889_001784"
     CDS             16141..17982
                     /locus_tag="LK889_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK5096018.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18006..19115
                     /gene="rpoD"
                     /locus_tag="LK889_001785"
     CDS             18006..19115
                     /gene="rpoD"
                     /locus_tag="LK889_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK5096019.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19335..21434
                     /locus_tag="LK889_001786"
     CDS             19335..21434
                     /locus_tag="LK889_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK5096020.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21630..22493
                     /locus_tag="LK889_001787"
     CDS             21630..22493
                     /locus_tag="LK889_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK5096021.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22598..>23052
                     /locus_tag="LK889_001788"
     CDS             22598..>23052
                     /locus_tag="LK889_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5096022.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7761 a         4037 c         4806 g         6448 t
ORIGIN      
        1 ttagaaaaaa ataactggaa caagagctat ttgtttttac agcaaagaat ggtatatttt
       61 tgtaggaggt ggcttacttg aaagaagtcg ttatgataga tgctgcaaga acgccgatag
      121 gaaagtatcg cggaagttta agtccattta ctgcagttga attaggcaca cttgtgacta
      181 aaggattatt agataaaaca aaactgaaaa aagataaaat cgatcaagta atatttggaa
      241 atgtcctgca agctggtaat ggtcaaaatg ttgccagaca gattgctttg aacagcggtc
      301 tccctgtaga tgtcccagca atgacgatca atgaagtttg cggatctggt atgaaagcag
      361 tgatcttagc acgccagctg atccaattag gtgaagcaga acttgtgata gccggcggaa
      421 cagagagtat gtctcaagca cctatgctga aacagtatca gtcagaaaca aatgaatatg
      481 gtgaaccaat ttccagtatg gtcaacgacg gattgactga cgcattttca aatgcacata
      541 tgggattaac cgcagagaat gttgcaacac aattttctgt gagcagagaa gagcaggatc
      601 gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt gtattttctg
      661 aggagatcat cccagtcaag atttctgatg aagacgtgtt atctgaggat gaagcagttc
      721 gtggaaatag tacattggaa aaactgggca cgttacgtac agtattctca gaagaaggaa
      781 ctgtaacagc aggaaatgct tccccgttga atgacggtgc ctctgtggtg atccttgcat
      841 ccaaagaata cgcagaaaat aataatctgc cttatttagc aaccatcaaa gaagtagcgg
      901 aggtcggtat tgacccaagt atcatgggaa tcgctccaat caaagccatt caaaaattga
      961 cagatagatc aggaatgaat ttatcaacaa tcgatctttt tgagatcaat gaagcatttg
     1021 cagcttcttc tatcgtagtt agccaagaac tgcagttaga tgaagaaaag gtcaatatat
     1081 atggaggagc aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt atcttgacta
     1141 cattagctta cggattgctt cgtgaacaga agagatacgg tattgcttca ttatgtattg
     1201 gtggaggttt aggcttggca gtcttgttag aagcgaatat ggagcaaaca cataaggatg
     1261 tgcaaaaaaa aaagttttat cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa
     1321 aaaatgtatt gacacaagaa actgcgttga tttttcagga acaaacactt tctgaagagc
     1381 tctccgatca catgatcgaa aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc
     1441 agaattttca aatcaatggc aagaaaaaat ggattccgat ggcaacagag gaaccatctg
     1501 tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc
     1561 agcggctgat gcgagggcaa atcgttctat caggaaaatc ggagtatcaa gcagtgatca
     1621 atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt tatccttcaa
     1681 tcgtgaaacg cgggggcggt gttcaagata tctctactcg ggaattcatg gggagtttcc
     1741 atgcatactt atctattgat tttttagtgg atgtaaaaga tgcaatgggc gctaacatga
     1801 tcaactcgat tcttgagagt gtggcaaaca agttacggga gtggttccct gaagaagaga
     1861 ttctattcag tattttaagt aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa
     1921 tcccattcga acgcttaggg agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc
     1981 aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac aacaaaggga
     2041 tcatgaatgg tatagaagct gtagtggcag ctacaggaaa tgatacacgt gcggttagcg
     2101 cttcgatcca cgcctacgcg gcaagaaatg gattatacca aggattaaca gactggcaga
     2161 tcaaagggga taaactagtt ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg
     2221 gagcttccaa tatcctgcca aaagcaaaag catctttagc tatgttagat attgattcag
     2281 ccaaggaact agcacaagta attgctgcag tagggttagc tcaaaacctg gctgctttgc
     2341 gtgcattagt tacagaagga atccaaaaag gccacatggg acttcaagct cgttcattag
     2401 cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag
     2461 cagaaaagat gaaccagcag acagctatac aaatattgga aaaaatccgg gaaaaataaa
     2521 aaagcaatat ctttagaaga ataaagcaag atttacagtt attttattta ctgcagatct
     2581 tgcttttttc tctatcagaa aatatgttta ttattagaat tttttatagt taaggttagg
     2641 catcttaaga aatcaggcaa ctccaagata tcttcaggta gaagagaagt cgcctaacat
     2701 tgtaggatca taggtgattt gttggacagg tcaagctgat atttgagcct gatgttagaa
     2761 ggaatttctt tttacttgct gtagaaatta ttatatgact aaaaaggctg ggattttaaa
     2821 tcccagcctt ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt
     2881 gttcgatcaa aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga
     2941 taacgaactc attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat
     3001 ttttttcatt aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag
     3061 caacggcttt tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat
     3121 tttcagttgt gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt
     3181 ctgtaactgg tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat
     3241 tccagtaaga cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt
     3301 ctcctgaacg aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc
     3361 gtgtatcttc atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat
     3421 caagcatgac ttgtaacggc aaaccataac gtcgcaagct ctcttcttct agagaacgga
     3481 ataattgtcc tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct
     3541 gttttttctt catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag
     3601 ccaaatgaaa aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt
     3661 tgcgtatttt cccgtttttt gaaagaaaaa taaaaaatgg aacggattat gtatatatac
     3721 ataatccgtt ccattctttc tattttttcg gtatcaaggc aaaaacaggt agttcaggtg
     3781 aataaatgac ttcatgttcg tgccagggtt tgaccattag cgaatgatcg aaccgttttt
     3841 tcagttcttc gatgatttga tcatgtgtcc agtttcttgc aggaaaatgt tggattttct
     3901 ctgcatcgat caaaaagaca aaatcggaat gaaaagtctg ggtaacttct tccgagagag
     3961 aattcaagcg ttcgattgga tccataaaat cacctttcga cttaaaattg ggtagtatct
     4021 gggtcttttg ctttgccgac tacaccatct aactgatcga tttgtttcat ttcttcttct
     4081 gttaattcaa aatcaaataa ttcagtattt tctttgattc tgcttggtgt cactgactta
     4141 ggaagaggaa ggaattcgtg ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg
     4201 tcatgtgctt cagcgatttg tttcatttca ggcacatcga agattttacc agtgcctaat
     4261 ggactatatg cttcgaggat catatcatgt ttttttgcat actcaactac agttgtctgt
     4321 aattcgcctg gtgcaaggaa aacttgattg accatcggca taatttttgc tgtttttgct
     4381 agtgtatcta gatggtgggg taagaaattg ctcacaccga ttgctttgat tttacctgct
     4441 tcatacagtt cttcaaatgc tcgccatgta tctgcatttg cttgttccca attatcacga
     4501 aaagcaacag ggtttggcca gtggatcaaa aacagatcta aataatctgt ttggagcttc
     4561 ttcaatgatt cttcaaaaga agacatcaca agttcatagc tgtggtttgc attccataat
     4621 tttgttgtca agaatatctc ttctctagga attccacttt ccttaatcgc ttggccgacg
     4681 ctttcttcat tcttgtatcc ttgggcagta tcgatatggc gataacctgc agccagcgct
     4741 tctttaacag aggaaacagc gacatcccca tcaggtgttt gccatgtgcc gaatcctact
     4801 accgggattt cgtaaccatt tgatagacga taagtgcttg ttaatgaatt catactttca
     4861 gctccttttt tattcgttca actataagtg tagcgcattt ttcgaaaaaa agaatccatt
     4921 ttgtttctac tcttgaaatt tttgtttaaa agaaaatgat tttgattctg gtatttttga
     4981 ttttttttcc gtaaactaag acagaaggga atgagaaaat ggatatctat ttaaaaaaag
     5041 caattctgca tattattgac agagaaactg gttctccagt cttttcacaa aaagagctgg
     5101 atctgacaaa agaatatatt cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg
     5161 ctcaaacaaa aactggacaa ctagcagaag aaagcatgat cgttgaatca cttaaaatcg
     5221 ctcaagaaaa ttttgtggaa gcaagtgaag caatcgttca gcggtggttc gatatctacc
     5281 aagaaagtga agaagcacct agtgcagatg tatttgttgt cttgtatgaa ttagatacag
     5341 tgatgtactt atctttattg aaagtcaatt atcgtgaggc ttacactcac tttgttgaag
     5401 cagacgaagc agggatcgac aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa
     5461 cacaaaaagc agatgaagca ttcgctgtga atttgactga ttggacctac gaattaattg
     5521 aaaaaaaata tgagttttct ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa
     5581 gtgttcctgc accttcttta gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc
     5641 ttggaaaaaa atttgagaca gaagaatttg atatcatggc tgatttgaaa gaagctgtct
     5701 atgatacgat agaagaaaaa ggtcggttgg atcatgagat gattgcggaa aaagtcttca
     5761 aggaaaacat cacggcaaaa atggctttta aagaagaagt acaagagcaa ggatttgtcc
     5821 cagaagcacc tcctgtaaaa gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa
     5881 aattcaagct atcaaacggg atcgaactga tcgtaccagt agatgtatat cgcaacccag
     5941 atttgattga atttgtgaat aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg
     6001 atgaagtatt gaatcgttta taaaaatgac tagaatatta ggtgttcaag taccagtgat
     6061 cagttaaatg aattgtgtca agagaaggat cggaaaagac gaatataaca aaaaaagagc
     6121 gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     6181 cagaagcaac tccttcttat ttcttggagt taaacgcttc tgtcccagcc tctttttatc
     6241 taataaaatt ttaagaccat ttactaatta tcgatttttt ctgccgcatt tttaggcact
     6301 tcagaagcct ttatttcttc ccagcttaat acacctttac gaggattgac tttgagttta
     6361 agatacgcat tcatctttaa tggatgttct ctttcttcgc gaatcgtcac ctgttttttt
     6421 tcaccatctt tattataagc cgcttgcttg taggtataag tcgtgaattt ctctccgtta
     6481 tctgcttggg tagctgacat ttcgccattc gtcgtgattt ttgtataata agcttcgccg
     6541 ccgtaaaaat aatgataagc tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt
     6601 acgccaatta attttttcat gtacttcacc tctttcctta ttattaacga aatagaagat
     6661 ttcgtctatt gaaagttgtt ggcaacagct gaattttaaa cttttcctca ttttgttctt
     6721 attgcttcaa aagaaagtta aggactatct tgaagttttt ctcaaaaaac acattttttt
     6781 atttataaaa ggtacttttt tcgcgtataa gacagacgag tgctcttagt tgactttgac
     6841 caatattgac gtataataag cttagttcga atcttacaaa aagtaagtcg gacgtattct
     6901 ttttgattta gtcaaaaaaa gactataata aaaaggtgag agctgaaatt tttcagtgat
     6961 ctatattatt taggaggttt gtaaattatg gcaaaagaac attatgacag aagtaaacca
     7021 cacgttaaca ttggtacgat tggacacgtt gaccatggga aaactacttt aactgctgcg
     7081 atcactacag tattaggcaa aaaaggccta gcaaatcctc aagactatgc tagtattgat
     7141 gcagctccag aagaacgtga acgtgggatt acgatcaata ccgcacacgt agaatatgaa
     7201 acagaaaaac gccactacgc gcatattgat gcaccaggac acgcggacta tgtgaaaaac
     7261 atgattacag gggcagcaca aatggatggt gcaatcttag ttgtttctgc aactgacggt
     7321 ccgatgcctc aaacacgtga acacatttta ttgtcacgcc aagttggtgt aaaatacctg
     7381 attgttttct tgaacaaagt tgatttagtc gatgatgaag aattgatcga tttggtagaa
     7441 atggaagttc gcgagttatt gagcgaatat ggtttcccag gcgatgacac tcctgtgatc
     7501 aaaggttccg cattaaaagc attgcaaggc gatccagatg ctgaagctgc tattatggaa
     7561 ttgatggata cagtagatga atatatccca acaccagaac gtgatacaga taaaccatta
     7621 ctattgccag tggaagacgt cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt
     7681 attgatcgtg gtgctgttcg tgtcggtgat gaggtagaga tcgtagggat caaacctgaa
     7741 acacaaaaag cagttgtaac aggtgtagaa atgttccgta aaacgttaga ttacggggaa
     7801 gctggggata acgtaggcgt gttgttacgg gggatccaac gtgacgatat cgaacgtgga
     7861 caagtacttg ctaaaccagg ttccattact ccacatacaa aattcaaagc agaagtgtac
     7921 gtgttgacaa aagaagaagg tggacgtcat actccattct tcaacaacta tcgtccacag
     7981 ttctacttcc gcacaactga tgttacagga acaatcacat tgccagaaga tacagaaatg
     8041 gtcatgcctg gtgacaacgt gacgattgat gttgacttga tccatccaat cgctgttgaa
     8101 aacggaacga cattctcaat tcgtgaaggc ggacggacag taggttcagg tatcgttaca
     8161 gagatcgaag cataagaaac gcgactcgaa tattttggta atgaaaaatg tttaaaataa
     8221 aagagcggtc caaacaggat ttctgtttgg accgctcttt gttcttcaat tgtataagaa
     8281 gagattgtag aagcatgaac tttcgtttta cttcagtctc ttttattcat catgagagtg
     8341 ttcatctatc ggtatgcagt ttttaatact ttcttcgtat ccattttttc aaaccactta
     8401 ttctaccttg tgtctgctca actggcgcat tatcaataat cagcaaagca atcataacat
     8461 atgcaatcca actatctcta gaatatagag tataacgtga gacccagttt gtagcgtact
     8521 tgcttttata ttctcttaat ccatggaaag aataaaaatg agaaccaaac tcgtaaacca
     8581 agtatgcaat cctttcctgt aaaaaactct ttctggaagt tccgacattt gaaagagggg
     8641 ccatccctaa attgaaccat tggatatgtt cttctttcat ataggcaaac aaatgcaaga
     8701 acaagaaatc catactgcca gaaggtgctt ttttagaatc atatcgcatc agatcaatcg
     8761 ttcctacttt attgttcgtg taagtaggca tgatcgttgc aaaagcgaca atttcttctt
     8821 gggtattttt gacaactgca ataggcgctc tttgtagata atcttcagag aaaaagccta
     8881 aagaaaaccc tttttccttt cgtgaaccca accaattatc agagatattt ttgaaagtac
     8941 tcatttgctc agtagaaaat ggtggttgta agacatcaaa tgtaaatcct tctcgttcga
     9001 tccgattcaa gacagcacga gtgcccttca tttttttccc tgaagttgta aaactattca
     9061 ggtctacata agcttcttcc cccatcttaa tgaaatcgta gccgaattca tgcaaaatca
     9121 taacaatctc ttcgcttgtt tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt
     9181 cgataaaagc ttcgattgct tctggaaaat cttctttctt accagaagga tcacccatta
     9241 caatacattt attattgaag caagcgaatt ggagaagaac agttggttca ccgtcttttt
     9301 cataggcaaa catccgctta tcttttaaga agatcaattg actatcggag tttcctccat
     9361 aagttgtaag gatcttcagg gccttttctt cattaaaggc ttcgccaatc tgtttctttt
     9421 ctccttgaag gaaatgaaca aatagaacga tcatgaaact aacagcgatg atggccaaca
     9481 atcccgaaaa ccaaatcttt tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc
     9541 gatgagggaa atccggcaga ttgtataccc caataacgat gtaaagcaag gacagtgcac
     9601 caataatgat tccatcgatc gtacgccact cccatgaata gatcaattgt tcccgaaata
     9661 attcgttttt agaagcaaga atgatcagaa gcagaataga caagaaaacc accggtgtga
     9721 aactaaaatc acttaataaa acataaagta aagctagaac aatcaagaag attgtaggca
     9781 aatatgctcg tttcacacgt gctgcgatcc ctctgcccat aatgattaaa agaaatccca
     9841 atagtatact aggaaattgg ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc
     9901 agcgaaactc tgtaaaagct tgcgggatag tggcagaaag tactagcatg attccagaaa
     9961 agtataataa gacaaccacg attttatgcg caatttctgt tgctaattgt ttagggatac
    10021 cagaataacg ctgatcaaaa gtggaaccga tatttttgaa gaaaaagacg attccaatca
    10081 aaaacggaat gatgtagtag aacaggcgat agagcaagat ccacacaacg actatttctc
    10141 ttggcacacc aatagcggac aacccgataa tcatcattac atcgaaactc ccaatttctc
    10201 ctggaatcat agacacgatt cctatcactg aagcagcaat gaataatgga attgtttgcc
    10261 acagatcaat ccggatttcc attaaataac caacagaaag gaaactaaac aggacaccgg
    10321 tccattccaa caatgaaact agcaatagct gtccttgagt tttaagtgag gtaccaccaa
    10381 ggaatccttt ctttttgaaa gtagtaaata aaaagacgac aggaaaatat agaccgccgc
    10441 cgatcaacca tatccaatat tgcttcaaaa aaggagcaac tggcgtgaag actacaagta
    10501 aaaaagaaag cagactgtaa atagaaagtc cagccatcaa aaacaagaaa actttcgata
    10561 atgcttggat cacgtttctg ctctctttct tttgtccata tagttcagag cgaagcccca
    10621 cactaacaaa cccgccgaaa ccggcaatat tgttgatcgt attgatcagc cagcttgtct
    10681 caaacaggta acgaggattc tgcttttgtt caagcatccg attcaaaata atatcataac
    10741 caaccatagg taacacagag ataagaccaa tcaatagcat tagtccgctt ttccatacag
    10801 gtatagtggc aaacgtttct cctaattgct ggatagatag agttttaccg atggacatca
    10861 attcaccgac aataatgaca atgacagaaa tcaaaaagat tgttttgaac agtcctaggt
    10921 ggtttttcag ccattgtatg gtatttttta acaaataaat caccttctat tcctaatagt
    10981 tatgtttcag aaagaaattg aagaacttct cgattgaagc gttcaggatc ttttctagct
    11041 aattcatgtc cttgttcttt cacaagcaca aaggaagctt ttggtatagt tttagctata
    11101 tacaaagaat gttttagctt gattacatct ttttttccca cgatgatcaa agtgggggag
    11161 ttgatttttt ttaaatcatt ttctgtaagc ccgatgtcat gcagcaaaag tttgataact
    11221 aaaagatttt tgcgaagact tgggcgaaat aaagagagta accataccca tgcataatga
    11281 atattactga tgactctggc agaaaaacga acgcctttaa caagcgtgtt tccagaattt
    11341 aaaatcaatc gatgaacttt tttagggaag ctgctggcaa ataccagtgc tagatttgct
    11401 ccatcactaa atccaagaaa atctgcctga tcgatttttt ccagcaacat gatcgtattc
    11461 agatcctcgg ccattaactg aaaattcaac atagatgctt cgtttgtcga acgaccatgt
    11521 cctcggctat ctactagata cacggtatag tagcgttcga gtacagggac ctgttctgaa
    11581 aaaaagcggc cgctgccgtc attgccatgc agtaaaaaga gagggaagcc ttgaccgctc
    11641 ttttcatagt aaatttcgct cccatctggc atttttgcat atttcttttc ttttttcaca
    11701 taatcaccta ctgtgtaaag tataacgatt atatcagtca ttgggcaatt gtttttactg
    11761 aaaaacaacg atcattttcg taagatgtca aaaaatacag gaaaattcat tttttcttaa
    11821 ggcgtacaca gaaatcaagg aagaaatagc caagaagata aagaaatagt aagataaaaa
    11881 aagatgtcaa aataactagg tgatgaaatg aagcgaaaac gattatctat tatccgtatt
    11941 cttttttaca tgatcattct gacggttatt ggtgcggtgt tgtttttatg aggaagaact
    12001 tatcgttcgg taaaacgagt ggaaagctat caaatagaaa taacaacagc tgcaaaaaat
    12061 tatcatatgg aaaactacga gaatttgatc aaggcgataa tattgaccga atcaaaagga
    12121 aaagggacag atctcatgca gagctcggaa agtctgcatg gagaagctga gatcattgat
    12181 aatccagctg acagtattga tcaaggtgtc aaatatttgg cggaaatgat agtagaagca
    12241 aagaaacaag gatgcgattt atggacagct gtccaagcat ataattttgg gaaagattat
    12301 attgcctatg taaaaaatca tggcggagaa aacacagtag aattagcaga agcgtattct
    12361 aaagatgtgc tgagcccact tttaggaaat gaggaaggga aacaataccg atattggcgg
    12421 atccagtcct tgctttacaa tggcggctat ctctatcata atggaggtaa catgttttat
    12481 cctgatattg tcaaaatgaa tgaaaaaata atagagacct atgaaaaagt ctttaagtaa
    12541 aatagcagga aatgttcaat catgaataaa attaaatgaa agaaggagtg cgcaaatagt
    12601 ggaaaaaagg caaaaaatgg aaaatcaaga agtggggtac acaaccaaag actggatgtt
    12661 acggtttgtg aagggaatgt ttatcggttc aggatttatc ttgccaggag tcagcggtgg
    12721 tgcattagca gctgtctttg gcatgtatga gcggctgatt tcttttttag cccaccttac
    12781 aaaaaatttc aaagaaaatg ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg
    12841 catctttcta ctttcttttt tagtcagttt tttattaggt gcgtatgaaa caacgatatt
    12901 atggtttttt gtcggatgta tcgctggaac tgttcctgcg ttatggaaag aagcagggaa
    12961 aaaaggaaga gatacttctg atgtgatcat catggttgtt acttttgttc tggcttattt
    13021 gtttttatta tacggcgctc aattatttga cgggcaagtc gatcagaatt ttggaacatg
    13081 gctgattgcc ggcggattga ttggtttggg tatgatcgtc ccaggattga gcccttctaa
    13141 ttttttggtg tatatgggat tgtataaagc gatggctgac ggatttaaag aagtacgact
    13201 tgaagtgatt gtgccgatcg gaattggtgg gattctttgt gttcttggat tgtcaaaaat
    13261 catggattac attttctcaa aggcatatag caagctattc catttcattt taggtgtagt
    13321 ttttgcttca acgattatga tcattcctac cgattacgcg ggattgggag ttgctggggt
    13381 actagcttgt cctgttcttt ttatagccgg tacagcacta ggttggtgga tgagccgttt
    13441 ggaggaacaa tataagtaaa gatagattga taataaaaac agcacacggg tgattcaaac
    13501 aaaaaggttg gcatcttttt tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa
    13561 acaagttgtt tttaatcttg gaacacgcta gacttaaaca cgtgattatt tttgaaagtt
    13621 ggcggaaaat ggagaaaaag aaaatgaatg gacttacaca gcaagaggta gttcagaaaa
    13681 tggcttctgg ccagcaaaat gactaccagg aagatgcatc aaaaagtaca aaacaaatat
    13741 tcagcgataa tatattgacg ttattcaatt ttctgaactt tggtattggg atttgtctgt
    13801 tatttgtagg tgcgtattcc aacatggcgt atttagcaat tattgtcgtc aatatcgtta
    13861 ttggaattta tcaagaaata catgcaagaa atcttgtacg gaaattatcg attgtttccc
    13921 aatcagaagt actagtgatc cgggacggta aagaacaatc gatttctact aaagagctgg
    13981 tattaggaga tttggtaaaa atcggagcag gagaacagat accagccgat ttacaggtta
    14041 tatctggacg ggcagaagca aatgaagctc ttttgacagg cgaatctgat ttgattgtca
    14101 aagaaactgg agctgaattg ctttctggaa gctttttgac aagcggttct gtcatggctg
    14161 aagttatccg agtaggtgca gataattatg ctgtccgctt gactagcgag gcaaagactc
    14221 acaaagctat tcgttctgaa cttgtagatt cgattcgcaa agtatccaaa ttcactagtt
    14281 tcgtgattat tcctttgggg atcatactat ttttggaagc actgttcatc cgcgatgctt
    14341 ctatcaaaat ttctgttatt gcatctgcag cagctttgtt aggcatgctt ccaaagggtt
    14401 tggtattgct aataagtatt gcattaacga caggggtaac caaactggca aaaaaacgaa
    14461 tccttgttca agacatgtat tcaattgaaa cgctggccca tgtcgataca ttatgcttgg
    14521 ataaaacagg gaccattact gaagggaaaa tgagtatcca gcgggtagaa gcactacatc
    14581 cgctgtacga gaaagccatc ccagaaatta tggggacgta tctggcagaa agcagtgata
    14641 acaacatcac tatgcgggct atgagagatt attttgagct ttctaatcgg tatgatgtga
    14701 caaacaaagt cgcattttct tctgatcgca aatggggcgc gatggaattt ccagaactgg
    14761 gagtcgtgta cgtaggagcg cctgaacgtt tggtggaaga agatcggctt ccggacagtc
    14821 taaaaacagc ccaagcgaat ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc
    14881 aattgaacga agaaaaacca gcatttgttg agccggttgc tctgtttgaa atcgatgatc
    14941 caatccgaaa aaacgctcaa gaaacactag cttatcttca tgaggaaggt gtggatctga
    15001 aagtgatttc tggagacaat ccttttactg tctccaatat cgctagacgt gcaggattac
    15061 cgaattatga tgcgtatgtc gatctttctc aaattactga agaaatggaa gtgcgagaag
    15121 ctgctcatcg ctatacggtt tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa
    15181 atgaattgaa agaaagcggc agaacggttg caatgacagg cgacggtgtg aatgatgttt
    15241 tggcgttaag agaagcagat tgcagcattg ctatggcaga aggtgatgga gcaacacgtc
    15301 agattgcgaa tctggtattg ttggactctg attttactac attgccagaa gtcctgtttg
    15361 aaggacgccg agtggtcaac aatgtgacga aagtctcagg catctttttt atcaaaacga
    15421 tttattcatt tattttatcg attatatgtg ccgttacagc aattggattt ccgtttatcc
    15481 ctatccaaat cacgctgatt gatttagcga ttgaagggta tccttccttc tttttatctt
    15541 ttgaacaaga taaaagaaaa atcacttacc gttatttgcc tacagcactt attaatgctt
    15601 taccaaatgc cttgcttgta gtagtgaata tcattgcggt gtatctactt ggaaaaacat
    15661 tcgcatgggg aaatgctgaa actactactt tgatgtatta cttgctgatt gggatcagtt
    15721 gcatggcagt tgtaaaagct tgttatccct tcaacccatt gcgcatcttc ctagcagtaa
    15781 cgacgatcgt agggatatat gtagctgcaa tgctgttcca tggaattttg gaagtaaact
    15841 tagggttggg agagagcgta ggatacttct ttgtattcat gctcgtaaat attgttcttc
    15901 gagtattgta ctggaaggct gatattcaaa atcagttact gaaaagagta gcaaattgat
    15961 gcttaatgag atagaaatgt gttatacttg aaaaagcaat gaaatatgat acaatcaaac
    16021 atgtataatt gtgtccttta aaatcgcact gatttcagtt agtgcgattt tttaccggtt
    16081 attctcctga aatatcttct gttaaaccca aaagatatca atagagcagg gggtgaaaaa
    16141 atggctcaac ggattcctca agaggttatc gaaacagtac gcagtcaaac gaatatcgtt
    16201 gaggtgattg gacaatatgt ccagttgaaa aagtctggta aaaattactt ggggctgtgt
    16261 ccttttcatg aagaacgtac accgtctttt tctgttgcag aagacaagca gatttttcat
    16321 tgctttggtt gtggtaaagg cggaaacgtg tttacttttc ttcaagaact agaaggtcta
    16381 agttttccag aagcagtggt aaaagtggca gacttcgaac agataccgtt agaagatcaa
    16441 tggcgtcagc gtactatagc tgtccaaaat cctgaatctg ctacggggaa actgattgct
    16501 gctcatgaaa aagcagcgga aatctatcat cacatgctat tgcatacaaa agctggacag
    16561 ccagctttag aatatttgca aagtcgtggg ttgtctttag aattaattga agaattcaac
    16621 ataggttttg cacctaatga gcgttctttt cttcagcaag tattcaagaa tgaatcaatt
    16681 tcaaatgaat tgttagaacg ctctggattg tttgtcacgc atgatgatgg ccgtatgtct
    16741 gatcgttttt atcaaagagt gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt
    16801 ttttcaggac gctggcttgc acctgaaaaa gggcaggaaa aagatcagcc caaatacttg
    16861 aatagtccag aaacagaact gttcaataaa agacaagttt tatttaatct tgataaagca
    16921 agaagcgata tccggaaaaa cggagaaatt ttgttgttcg aagggtttat ggatgtgatt
    16981 gctgcatggc aggctggtat caaaactgga ttagcttcta tggggacaag tctaacagct
    17041 caacagatcc aacaaatgga aaagctgacg aaagaattgg tcttttgcta tgacggagac
    17101 aacgcaggtt ttgaagcaac caatcgaggg attgaattgc ttcggcaaaa cagtcgatta
    17161 gcactttctg tcgtagtcgt tccagaaaag cttgatcctg atgaatatct ccgtaagtat
    17221 ggagaagcat ctttccaaga attagtggct catgggcgcg aaacagtctt tacttttaaa
    17281 tcacgttatt taaaacaagg gaaaaattta gataatgaaa aagacaagat cgaatacctc
    17341 gaagaattac tagttgaact tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta
    17401 tctcaactgt caacagagtt tcagatcggc agagagacga tacaaaaaca actgcgcgat
    17461 cttcgaagga caaatcagca aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt
    17521 acgagacaag aaactgcacg gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag
    17581 attctattat atcgtgcatt taaagaagtt tctgtccgta atattttaaa agaacgagaa
    17641 tttcagttta ttcatgatgc atatgcagaa gtttattttt tattcgatgc atatgtctca
    17701 caacattatg aattcaacct tgcagagttt ttagactttt tgcaagatga aaatttaagg
    17761 cggttggtgg tggaaatcga atatttaccg gtggcagaag aaagttcgcc acgagaaatc
    17821 caagacttgc tagatgtgat cagcaaatct ggcttagctg atgaaataac attaaaaaaa
    17881 caaatgcagc aagaagccag acgaactgga aacaaacagc ttgagcttga attgacgatt
    17941 gaaatcatca atttaacgaa acagttaaaa caagccaaat agctactcaa ggggggcctt
    18001 cttttatggc aaatggaaca gataaaaaaa cgtatgaagc agcagtagca gctttcatca
    18061 aagaaaacaa acccaaacga caagttgtat atgatgattt atccaatcag cttgctacac
    18121 cattcacttt gaatgcagat gaaatggaca aattgatcca aaagattgaa gacgcaggta
    18181 tcagtgtggt ggatgaaaat ggtgatccat caattcacag cttgaagagt gctgagaaaa
    18241 aagctgaaca agcaaaaaca gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc
    18301 cggttcgtat gtatctgaaa gaaattggcc gtgtctccct tttaactgcg gaagaagaag
    18361 tagcgttagc attgaaaatc gaagaaggcg accaagaagc gaaacagcgt ctggcagaag
    18421 caaaccttcg tttagtagta agtatcgcta aacgttatgt aggacgaggc atgcagttcc
    18481 ttgacttgat ccaagaagga aacatgggac tgatgaaggc agttgaaaaa tttgactacc
    18541 gcaaaggatt taagttttca acctatgcta catggtggat tcgtcaagcc atcactcgag
    18601 caatcgctga ccaagcacgt acgatccgta ttccagttca tatggttgaa acaattaata
    18661 aattgatacg tatccaacgt caattgctgc aagaccttgg aagagaacct acgccagaag
    18721 aaatcggtgc ggaaatggat cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc
    18781 aagaacctgt ttcccttgaa acgccaatcg gtgaagaaga tgactctcac ttaggtgatt
    18841 tcatagaaga ccaggaagca acaagtccag cagaacacgc agcttatgag ttactaaaag
    18901 aacaattgga agatgtacta gatacattga ctgatcggga agagaatgtc cttcgcttac
    18961 gttttggttt agatgatggc cggacgagaa cattggaaga agtagggaaa gtatttggtg
    19021 ttactcggga acgtattcgt cagatcgagg ctaaagcatt aagaaaattg cgccatcctt
    19081 ctcgttcaaa acaattaaaa gatttcttag aataagcgtg gactattgac cagaagagaa
    19141 atcagtaaaa ctgtttggtt tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa
    19201 gctttttttt ctataggaag gaaattcatt cattattttt aagtaaatcc taaaaataag
    19261 ctattggcgt gacgtataga atatgataga ttaaatggta gcgtaaacct agtaaaggag
    19321 ccgatcgcta agtgatgaaa aaatgtccaa attgcggatc gaaaaaaatg gagcagaata
    19381 ctatctgtcc agactgcggt catcaaataa ctgcagaaga tcagcaaata aaagaatcaa
    19441 ttgaagaaac aaatacagat gcagctatgc aggataaatc aactgaagat gcagtctttc
    19501 ctgatacaga attaaacgat ccaatcgaat ggtcggaatt gaaagattta cctctagaat
    19561 ctgtaatgga attattcgaa aaagcagaac cagaagacga agaaccacct gctcacgatc
    19621 aagtaactaa tatagagaaa aatgaacaga agaaagcaac tgttcatgag acttcggaag
    19681 aatcaggaaa gaatcaagaa gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac
    19741 tgaaagaaat cgtcgataat gaggaagaaa attcgatact atccgcttat atcaaagcac
    19801 atcgtgaaga tacaaaagaa gagcacgcga aagagctgct caaaatgatc agtgaaaaaa
    19861 ttgcacagga aaacaaagta gagccagaaa aggaacaaga aactgaaaaa gactctgata
    19921 gagaaaacgc cacaggtaaa gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag
    19981 aaaaaactga agtatttact gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg
    20041 aagcagagaa aaaagaaata ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa
    20101 taaatagtga atccgatgct gaaaatcaat ctgtaagcaa agagggtact gatcttgaag
    20161 caaaaataga aaaagaagag aaccagccta agctgtttga agcagaagcg aaatcaataa
    20221 caatcgtgcc agatactgaa aaaacacatc aagaatcaaa cttagaaaaa acagatccag
    20281 ataaagcgca aaagaaaaaa tcaaaaaaag taccttatct cgttttagca gcagtacttc
    20341 tactcggtgg aggaagctgg gcttattatg accatcatca aaaagtagaa gccgaagtag
    20401 cagcagaagc aaaaagacag caacaaatgg aagaacttca tgagaaactt gctgcctttt
    20461 atacagatga tcaccatcaa tttattcgtg cagatatgat tcatcaagat ttatcaaaac
    20521 taaaaaatga tttatccagt cttaagaatg aaaaaggata ttcagagtta gaaacaactt
    20581 accaagatat ccaatctaag atccgagata tccaaaaagt gaatgagtgg ttcatagcac
    20641 ctgccatcgt cgatgatcat ctagcagatg acatcaagtt gaaagcagat aaagagatcc
    20701 aagcaatcga aacggataac acagattttg gaaaattgat tcaacaagca caaacagaag
    20761 ctaaaaatca atatgctgaa ttgcaaacag ctaaagaaaa aacagctata gtctttaaag
    20821 acggaaaagt agtggattca gcaacacgcg accaatacaa gacggcaaaa gaagcagtag
    20881 aaaaagtgaa aaatacagat ctatctgctc agttgaatga acaattgaaa caagtagata
    20941 agatgctcac tgataaagag aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga
    21001 aaaaagcagc tgaagaacaa gcaaaacaag ccgctcaagc agcagctgca gctcaagccg
    21061 ctcaacaagc tgcggtcgca caagcagcac agcaagaagc ggcatcctcg accccttcct
    21121 cttcaaactc agcgaatcag ccaatcaaat ctgccagagc aagtgatatt gccgatgcat
    21181 ctaatccagc atggacgtgg gcaccaggtg tccaaagcaa agtgattgct acatgtatcc
    21241 aacgtggata tatcgttgaa ggaggttaca gattagaaaa agccagaatc gaaaatgggg
    21301 aaggctatta taatctatat gccacttcaa caaaatctgc attgatgaaa ggcattggag
    21361 aaagtgcttt gccattttac atcgtaacca tcaactgtaa aactggatgg ttcggaggaa
    21421 atgggagcaa ttaattttag gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt
    21481 tattcgaaaa aggggagtat gacacaactg ttgtcatact ccttttttca cctttccaaa
    21541 ccaaggctca gctattcaag tagataacat tatgttatga tagcaaagaa atgtattaag
    21601 aaaacctttc actatagagg gagaaaaaaa tgaacgattt attagcaact atatttacag
    21661 gtattattat tgatgaaaat gaaaaaaact actttgtcca aaaaaacgga caaacctttg
    21721 gcttaagcaa atcagaaggt gaacacaaaa tcggtgaagc agttgaagga ttcggttacc
    21781 aaaatcaaaa acatgaaaat cggatcacta cacaaattcc taaaagccgt atcggtcatt
    21841 acgcttttgg aatcgttact gctacacgaa aagatttagg tgcgtttgtc gatatcggtc
    21901 ttccagataa agactttgtc gtatctcttg atgaattgcc gacaatgaca gagctatggc
    21961 ctaaaaaagg ggatcgcttg atgatttccc ttagagttga tgcaaaagac cgtatttggg
    22021 gcagtttagc agatgaaaaa atattcaaat ctttaagcaa atatggaaca gaagaaatga
    22081 aaaacaaaga tgtgatagga acagcatacc gcttgaaatt aacaggtacg tacgttttaa
    22141 cggaagattt ctatcttggg tttatccatc cttctgaacg atataatgaa cctcgtctag
    22201 gcgagcagat aaaaggtcga gtgattggtg tccgaccaga cggtgtactg aatctttcat
    22261 tgaagccaag agcatatgaa gctatatcag atgatgcaca aatgatttta accattttag
    22321 aacgttctgc agatcagcaa atcaacttta cagataaatc ggatccagaa gaaatcatga
    22381 aagcttttgg tatcagcaaa ggagcattta agcgcgcatt aggtaatctt ttgaaacaag
    22441 gattgattac acaagaaaac ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc
    22501 ttgcatgttt ttcttaacta gcttataata attttaatta acatagtgtt atatcttttt
    22561 ttatttgtaa ttattataaa ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc
    22621 gatgaaaaaa acaaaacagc agctacatga agcggggttt aagctgacac cgcaaagaga
    22681 agctactctg cttgtgcttt tggaaaatga gaaagagcat ttatctgcag aagaaatctt
    22741 ctttttagtg aaaaaaaaga gtccggaaat tggacttgct actgtttatc gaacccttga
    22801 aatcttgaca gaattgaaaa ttgtcgataa agtaagtttt aatgatggat tagcccgtta
    22861 tgatttgaga aaagaaggcg ccaaacattt ccatcatcat ttactatgct tgaactgcgg
    22921 cacgatcgag gaagtagaag aggatctttt acttgaagtg gagcaagtag tagaagaacg
    22981 ttatcatttt ttagtaaaag atcatcggct aacgttccat ggaatctgtc aggaatgcta
    23041 tgaaaaagaa aa
//
LOCUS       DAFBQW010000037        22857 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFBQW010000037 DAFBQW010000000
VERSION     DAFBQW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.038"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(104..1336)
                     /locus_tag="LK889_001789"
     CDS             complement(104..1336)
                     /locus_tag="LK889_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK5096023.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="LK889_001790"
     CDS             1591..2160
                     /locus_tag="LK889_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5096024.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="LK889_001791"
     CDS             complement(2338..2778)
                     /locus_tag="LK889_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK5096025.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="LK889_001792"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="LK889_001792"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK5096026.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="LK889_001793"
     CDS             3732..4655
                     /locus_tag="LK889_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBK5096027.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="LK889_001794"
     CDS             4731..5870
                     /locus_tag="LK889_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBK5096028.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="LK889_001795"
     CDS             5863..6663
                     /locus_tag="LK889_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5096029.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="LK889_001796"
     CDS             6663..7490
                     /locus_tag="LK889_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5096030.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="LK889_001797"
     CDS             7468..8202
                     /locus_tag="LK889_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5096031.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="LK889_001798"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="LK889_001798"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK5096032.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="LK889_001799"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="LK889_001799"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBK5096033.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="LK889_001800"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="LK889_001800"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK5096034.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="LK889_001801"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="LK889_001801"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBK5096035.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="LK889_001802"
     CDS             11787..13820
                     /locus_tag="LK889_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096036.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="LK889_001803"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="LK889_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="LK889_001804"
     CDS             15353..16159
                     /locus_tag="LK889_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096037.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="LK889_001805"
     CDS             16171..17391
                     /locus_tag="LK889_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096038.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="LK889_001806"
     CDS             17381..18967
                     /locus_tag="LK889_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5096039.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="LK889_001807"
     CDS             19006..21144
                     /locus_tag="LK889_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5096040.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="LK889_001808"
     CDS             complement(21512..22495)
                     /locus_tag="LK889_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBK5096041.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4712 g         7070 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtg
//
LOCUS       DAFBQW010000038        22132 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFBQW010000038 DAFBQW010000000
VERSION     DAFBQW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.039"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..523
                     /locus_tag="LK889_001809"
     CDS             <1..523
                     /locus_tag="LK889_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBK5096042.1"
                     /translation="GGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGIVA
                     NCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFGS
                     LIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTVL
                     LLPLFFQPFAGLF"
     gene            523..2109
                     /locus_tag="LK889_001810"
     CDS             523..2109
                     /locus_tag="LK889_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096043.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2128..2349
                     /locus_tag="LK889_001811"
     CDS             2128..2349
                     /locus_tag="LK889_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBK5096044.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2346..3107
                     /locus_tag="LK889_001812"
     CDS             2346..3107
                     /locus_tag="LK889_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBK5096045.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3487..3996
                     /locus_tag="LK889_001813"
     CDS             3487..3996
                     /locus_tag="LK889_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK5096046.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4071..4610)
                     /locus_tag="LK889_001814"
     CDS             complement(4071..4610)
                     /locus_tag="LK889_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBK5096047.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="LK889_001815"
     CDS             complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="LK889_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK5096048.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5623..6474
                     /locus_tag="LK889_001816"
     CDS             5623..6474
                     /locus_tag="LK889_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK5096049.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            6502..7137
                     /locus_tag="LK889_001817"
     CDS             6502..7137
                     /locus_tag="LK889_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBK5096050.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7157..7930
                     /locus_tag="LK889_001818"
     CDS             7157..7930
                     /locus_tag="LK889_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5096051.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8338..9135
                     /locus_tag="LK889_001819"
     CDS             8338..9135
                     /locus_tag="LK889_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBK5096052.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9113..9526
                     /locus_tag="LK889_001820"
     CDS             9113..9526
                     /locus_tag="LK889_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5096053.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9510..12155
                     /gene="mutS"
                     /locus_tag="LK889_001821"
     CDS             9510..12155
                     /gene="mutS"
                     /locus_tag="LK889_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK5096054.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12173..14281
                     /gene="mutL"
                     /locus_tag="LK889_001822"
     CDS             12173..14281
                     /gene="mutL"
                     /locus_tag="LK889_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK5096055.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14303..14863
                     /gene="maf"
                     /locus_tag="LK889_001823"
     CDS             14303..14863
                     /gene="maf"
                     /locus_tag="LK889_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBK5096056.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(14948..16936)
                     /gene="treP"
                     /locus_tag="LK889_001824"
     CDS             complement(14948..16936)
                     /gene="treP"
                     /locus_tag="LK889_001824"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBK5096057.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17159..17872
                     /gene="treR"
                     /locus_tag="LK889_001825"
     CDS             17159..17872
                     /gene="treR"
                     /locus_tag="LK889_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBK5096058.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            17949..18395
                     /gene="msrB"
                     /locus_tag="LK889_001826"
     CDS             17949..18395
                     /gene="msrB"
                     /locus_tag="LK889_001826"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK5096059.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18565..19167
                     /gene="ruvA"
                     /locus_tag="LK889_001827"
     CDS             18565..19167
                     /gene="ruvA"
                     /locus_tag="LK889_001827"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK5096060.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19180..20181
                     /gene="ruvB"
                     /locus_tag="LK889_001828"
     CDS             19180..20181
                     /gene="ruvB"
                     /locus_tag="LK889_001828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK5096061.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20210..20788
                     /locus_tag="LK889_001829"
     CDS             20210..20788
                     /locus_tag="LK889_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5096062.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20840..21322
                     /locus_tag="LK889_001830"
     CDS             20840..21322
                     /locus_tag="LK889_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBK5096063.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21439..21813
                     /locus_tag="LK889_001831"
     CDS             21439..21813
                     /locus_tag="LK889_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096064.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7237 a         3991 c         4790 g         6114 t
ORIGIN      
        1 cgggggcaat tttttgacta tgctgattac tcctgcgaca gtggcacttg ccatccctct
       61 ttataaaaac ttccgtttac tgaaagagaa tttttatcct gtaatggctg cgattttagc
      121 aggaatagtc gcaaattgct tggttagtgt agggttcggt tacttcttct ctcttcataa
      181 agagatggtg atctcattac tacctaaatc tgtaacgacg gctatttctg tcgatttatc
      241 tcatacgatg ggcggggtga atgccgtgac acttgctatt gttgtctcta caggtatttt
      301 tggttcgctg atcgccactc atattttccg cctattcaag atcgaaagtc cagttgctcg
      361 cggtgtggcg ttaggaagta cttctcatgc tattggtaca gcaaaagcaa tcgagattgg
      421 tgaaatagaa ggaatcattt ccggacttgc gatatgcgtc aacggaatat tgaccgtttt
      481 attgctaccg ttattctttc agccttttgc tggcttgttt tgatgcgaaa accactattt
      541 tatgctacaa taaccaaaac gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct
      601 tccgagtatc ttaaacaatt tgataaagat ctatattata agattctcct cctagaaagt
      661 tttgaggatc aagcttggca taccgctgca caattagcgc aagttgttca gctggatgcc
      721 cgcagcgtgt cgaaatacct gaacgagctg tctaaaaact atcagcagtt tagcggtaag
      781 acacaccctt tgtttacaaa aaatcaccgt tctggttata acttttatga tacgctcgat
      841 tcaattgaac atgagcgttt tttgatctat ctcgtgcaaa gtacgctgaa attccagttg
      901 ctgcacgaca ttttttttga agaattccat acaatgtacc aatttgcgca aaagcattac
      961 attagtgaat ctactgctca tcgaaaaatc aatgaatgga aacagcaact gcaaacttac
     1021 ggaattcggc tgcaacgagg tacatatatc gctcaaggcg aagaagaaat cattcgactt
     1081 tacttgcata tgacattctg gcagttgttt cgagggaaaa tctggccatt tgaaacgatt
     1141 tctcaaatgg acgtaaagaa tatggcagaa catatcatgg ctttttttaa tgttcgtctg
     1201 aacgagatca aaaaaagacg attggaatat atgctaggtg cctttttttt aagaaaatca
     1261 cagaagcatt atgttgtatt gaatgagaaa aaaagacggc tgatttctga caatctgttg
     1321 ttccagcgat tttgccaggt catggagccg gtatttccta attattttca agtagaagac
     1381 gagttaggcg cattatttct tgtactgatg actcgtgaag aatattatag tgatcctaag
     1441 atacggaaga aaatttttga ttttcatcaa caagcaaaaa caccgccctt tacagccctt
     1501 tcagaagcaa aagccgctct ttctctttat caggaagagc aggggctgcc agcagaaaat
     1561 ctaaccttcg aggcagaaaa ctatttattc agcagtcatt ttttcgccta tctttttcct
     1621 aatgcgaaag aaacgattga tggcaatagt agtgatttta ttaatcattt agttattgaa
     1681 aacaaggaat tgaaacaatg gctcgtacat ttttttgaaa gtagacacaa acatcccaat
     1741 catctggcat ttaaaaatca tgcgtttttg atggggcggt atctgactgt gtttaaaaca
     1801 ttaggagcct ttactccaca gcttccaaag atcacgatcc ttttaatgac agatttccca
     1861 ttatttgaag agcaactgtt agaggaggga ttacggacat ttttccgtaa tgagtatcag
     1921 ctgatttttc tgccaacaga ttatcggggg agagaagttg atcttctgat ttcgacaagt
     1981 aaagtccacc gcaaaccatg ggcggatctt gactatttta ttgtgactga agaactaaaa
     2041 ttaattgatt acatccaact atctcaaaaa tttgaaatga ttcaaaaaca aaaactatta
     2101 aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc agaattgaaa
     2161 aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt tgttctcgta
     2221 tccgatgatg aatttgatgg ctgtccggtg acgccgtact tattgatcaa aacaagtgac
     2281 gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga ttgggcgatc
     2341 gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc acaggagctg
     2401 cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct tttgtatttg
     2461 gattagatca aaagttcggt aaaatggaag aaatttcttt gcgatttcct gaaacttttg
     2521 cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta tcgaaatgcc
     2581 aaaatcattt tggtgaagtt acgattttac tgaatacagc ggggatattg gatgcctaca
     2641 aacccttgat ggaaactgac gaatcgctgt gggatctgat ttatgagacg aatgtgaaaa
     2701 gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccaaaaa agcggaacga
     2761 tcatcaatat ggcttcgatt gctggcttga ttgctggtgg aggtgggatc gcttatacaa
     2821 gcgcgaaaca tgcgattgtc ggatttacca aacaactcgc tctggattac gcaggacagg
     2881 gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat gcggctgatt
     2941 ttgaagggaa aggtgagatg gcagagtggg tggcaaatga aacaccagtc aagcgatggg
     3001 cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa gcaagctaca
     3061 tacaaggaac gatcatccca atcgacggcg gatggttgtt aaaataacct tgtgtttttt
     3121 tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa aaaaagtcga
     3181 tgactgttcc cgacattcga tgccgaacag ccagttcaaa aaattctttt taataatcgt
     3241 taatattcga aactaagcgg cttttcacga cctcttgata taaacgatat tcaaaagctt
     3301 gcaactgcgg taacaagtcc agccaaatga aaaatataag gagctatttt tatttgacca
     3361 ttcttattac ttgttgcagg gcagcttttt cactaccttc tgcgacactt cagttggaat
     3421 tttcttccca tcctttggtc gcggtcatta ccaagataaa attatcttgc aaaggagaaa
     3481 tagaacatgg aaaaaaataa tacccgttca gccaaacgct ggacaacgac agatgtgact
     3541 cggatggcgc tagtcactgg tttatatgtg gcagtgacgc tggttttgtc tgtctttagc
     3601 tttggagtga ttcagattcg tctctcggag atgttcaact atctgtcctt gtataacaaa
     3661 agatacatcg tcgcggtaac agcgggagta gctttggcta atattgcttc tcctcttgga
     3721 ctgatagatg ttgcagtagg aagcgtcagc acgctgcttg tattgcttat caactataaa
     3781 atcactagtc gcatcaaaaa catgaaaatc aaaatggccg tgacagcatg cgtatttgct
     3841 ttttccatgt ttacagtagc tggtcagcta acgattttat atcaactacc ttttttcttg
     3901 aattggtggg tgattgcttt aggagaattg gcatcaatga ttgtcggtgg gataattatt
     3961 tattggatcg gtaaaaaagt ggatttgtca aaataaagat ctgtttaatt taaaataggg
     4021 cagcatggaa gagactgaat gttgagtctt cttccatgct gcttttttga ttaatttgta
     4081 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     4141 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     4201 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     4261 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     4321 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     4381 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     4441 ccaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     4501 gtttgccctg tcaaaggaac caaaccaaaa ggaatggtca tctgagaaag gacagaaata
     4561 atcgcggcaa attccgcagc aagtatcaat tctttcaatg ttcggttcat aacgccctct
     4621 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     4681 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     4741 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     4801 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     4861 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     4921 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     4981 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     5041 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     5101 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     5161 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     5221 gtattctgat tgtttgccac ggctattagg tccatgttga cctggtgcgt aagggcgacg
     5281 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     5341 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     5401 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     5461 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     5521 ctgcattatt ttacacaaat tacagtatat gatagatcat ccacgaatgt caagataatt
     5581 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gtatgtgcaa
     5641 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     5701 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     5761 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     5821 gcgatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     5881 atgttcagct ggggtctggt catgattcta tacatgaacg ccgaattagg aacatcgaat
     5941 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     6001 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttctaatt
     6061 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     6121 gtaaaactag caaaaccttc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     6181 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgtcggaaa agtgatcaca
     6241 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     6301 cctgccttta cgcttactta ctttgcttct cacggacaga tggatggaat gacagtcagc
     6361 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     6421 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     6481 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     6541 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     6601 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     6661 ttagcttttg ttcattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     6721 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     6781 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     6841 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     6901 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     6961 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     7021 aaagcaattg gttgggcatt acgcgattat agtaagaccg atgccgattg ggtaaaaaga
     7081 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     7141 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     7201 agattcaaac aaacaagctt tagatagcac aagagaagca ttggaaaaat tacgcaaagc
     7261 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     7321 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     7381 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     7441 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     7501 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     7561 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     7621 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     7681 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     7741 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     7801 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     7861 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     7921 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     7981 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagctttcg
     8041 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     8101 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     8161 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     8221 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     8281 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
     8341 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
     8401 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
     8461 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
     8521 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
     8581 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
     8641 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
     8701 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
     8761 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
     8821 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
     8881 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
     8941 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gactgcatta
     9001 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
     9061 ttggatgata cgacaggtca tgcaaaagaa atccagctga ttcagatcaa cgatgatcga
     9121 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
     9181 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
     9241 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
     9301 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaaa aagaagcaat
     9361 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
     9421 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
     9481 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
     9541 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
     9601 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
     9661 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
     9721 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
     9781 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
     9841 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
     9901 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
     9961 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    10021 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    10081 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    10141 ttttgacaag cgaactagtc gatccgctgg aaaaagaagc aaccggcaaa ctattgactt
    10201 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    10261 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    10321 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    10381 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    10441 aagcaagaca agaaatggtc gcttctttgc tagacagcta ttttgaacgt atcgatttgc
    10501 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    10561 atgtcaatgg gcgagacttg atccagctga aaacttcttt agaacaagtg ccaatgatca
    10621 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    10681 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    10741 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    10801 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    10861 gaatcaagaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaattacaa
    10921 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    10981 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    11041 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    11101 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    11161 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    11221 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    11281 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    11341 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    11401 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    11461 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    11521 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    11581 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    11641 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    11701 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    11761 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    11821 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    11881 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    11941 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    12001 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    12061 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    12121 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    12181 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    12241 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    12301 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    12361 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    12421 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    12481 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    12541 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    12601 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    12661 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    12721 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    12781 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    12841 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    12901 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    12961 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    13021 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    13081 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    13141 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    13201 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    13261 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    13321 cgtagaagaa ctaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    13381 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    13441 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    13501 aaagaaagaa tttcccattg tggataacat ggagaaaaca aatgaagaac aagcagataa
    13561 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    13621 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    13681 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    13741 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    13801 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    13861 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    13921 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    13981 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    14041 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    14101 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    14161 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    14221 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    14281 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    14341 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    14401 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    14461 aactgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    14521 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    14581 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    14641 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    14701 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    14761 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    14821 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    14881 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    14941 actctgacta agattttact tcagctttac cgactgcttc gcccgggcga acaagtcctg
    15001 attggtcaag agaaagtcct gcaacttttt cattattggt caaagcaaca ataatgtcag
    15061 ttttcttgcc agcttgttct atttctggca aatccatttc agcaatcagg gtctctggtg
    15121 tgacaaggta tccttctttc acgaatacat tgaaaggaac accctttagt tcaacagtat
    15181 ctaatcccat gtgaacaagt acttccagcc cctcattaga aagaatacca atggcatgtt
    15241 ttgtttcaaa gacactcgtc actttaccgt ttactggtgc atagactttg ccgtttgacg
    15301 gttcgacagc ataaccgtca cccatcattt tttgtgagaa aactgggtca tttacttcag
    15361 ttatttcctt gatggtccct gcagcgactg cgaataatgt ttcttttgtt gcaactgccg
    15421 taccagttgc tgaaacaggt tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt
    15481 gaagttgtac atcagcagca ttttcaggaa cagcaggatc aatcttattc aaaattccct
    15541 gacgtctaaa gatgatcgtc aagccaaatg gaatgatgac agcaatgatc attgcgatga
    15601 tgaaaggtac ccatgtttct gcttggatag ctagtattcc tggcaaaccg cctactccga
    15661 tcgcgttagc tcgaacaccc atcaagttag caaacatgcc agctaatcca gagccgacca
    15721 ttgcagcgac gaaaggatag acgtatttca aattgattcc gaacatagca ggttcagtta
    15781 cgcctaagta acaagaaatc attgctggaa tcgagatttg ttcttctttt ttattcccac
    15841 gatgtaagaa gatgatggct aagactgcag agccttgggc gatatttgat aaagcaatca
    15901 tcggccataa attcgttgat ccgaaatctg caatcagctg catatcgatg gcattggtca
    15961 tatggtggag accagtaatc actaaaggag catacaggaa tccgaatacg gcactaaaca
    16021 accagctgat agaagacgtc aatccagtat ttacgatatt tgagatccag cttccgactg
    16081 tccagccgat aggacctaaa ataacgtgtg cagctaaaac agttggtaac aatgaaaaca
    16141 atggaacaaa aatcatcgag atcgactgag ggatatattt gcggaagaag atttctagat
    16201 acgctaacat aaaccctgcc agcatggctg gaataacttg cgcttgataa ccaatcatct
    16261 ggacttgagc aaaaccaaaa tcccaaaatg gaatatctgc tgcggctgtg gaagctacac
    16321 tataagcatt taataattgc ggagaaacta acgtaattcc taaaacgatt cctaatattt
    16381 gtgtagtacc catctttttt gcaatactcc aagtgatacc tactggaaga aaatggaaaa
    16441 ttgcttcacc cggtaaccac aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa
    16501 cgattgttcc accaagactt tcaaactgga taccttcaag tacatttctg aatccaagga
    16561 tcaatccacc gacaatgatt gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa
    16621 tagcacgttg aacgggatga aggttttgtt ttgcagctgc ttttccttgt tctttggaaa
    16681 ccccttcaac tccagatact gcaacgaagt cgttataaaa agtagaaaca tcgtttccta
    16741 tgatgacttg gaactgtcct gcattcgtga acatcccttt tacgctaggt atatcttcga
    16801 tcgctttttc gtccgctttt cctggatcat tcaggacaaa acgcatacga gtcgcacaat
    16861 gactaactgc agcaatattt tcttttccgc cgatatcatg taacagcttc tcggcatctg
    16921 cttgatattt tgccatatca ataaacccct ttctcttaac ttgtctatgc aagttgtttc
    16981 ttcatttact atactttttt tcggagagaa ttgcaagcgt tttattaaaa agtacgagag
    17041 aaaatgatat cgttttaaaa agcttaaaat aagcttttct taaaaatagg aacattgaaa
    17101 tacaaaaaga gctgtagtat agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat
    17161 gaataagttc aatgaaattt ttttagattt ggaacagaaa attttagcga aagaatatcc
    17221 tccgcattcg cttttaccca gtgagaacca attgatcaaa atttatggtg tttcacgaga
    17281 aacaattaga aaagcgctga atcttttgac tacggaaggt tatattcaaa aaaaacaagg
    17341 aaaaggttcg atcgtactgg atcggaatcg cttcgacttt cctatttctg gattgactag
    17401 ctataaagag ctacaggaga cccaacgtat tccaagtgaa acgatcatcc atacgttaga
    17461 agaaacagaa gtgacaaaag cattgaacga gatcactggc tgggagatcg gtgcgcctgt
    17521 ttggcatttg atacgcgaaa gaaaaatcaa tggcgaagta gttatattag acaccgatta
    17581 tctattaaag gaaatcgttc ctattttaac tccagcacaa gcacaaggat cgatctatga
    17641 atattttgaa aatgagcttt ctttgacgat tgcttacgcg caaaaggaga tcacagtaga
    17701 agaagtaacg gaaatcatga aaacagcgat ggatcttcac gatgacactt atgcagtagt
    17761 agtcagaagc ctcgttcatc tggaagatac ccgctgtttc gagtattctg agtcggttca
    17821 ccgattagat aaattccgtt tcgttgagtt tgcccgacgc cgaaaagtgt aaatagttca
    17881 aaagaaagca aattgttggc atctgtccac tttggcgcta tgataggatc acaaaggggg
    17941 catttttaat ggataaacca ataagaccag aaaaagaaga actgaaagaa aagttatcac
    18001 ccatggctta tgcagtcaca caagaaaatg caacagaacg accatttagc gggaaatatg
    18061 atgattttta cgaaaagggg atctatgtag atatcgtcag cggtgaaccg ttattcagct
    18121 ctgcagaaaa atatgatgca ggttgtggct ggccatcatt cagcaaacca ataacgaaaa
    18181 gacaagtgcg cgaaaaagcc gatttttctc atggcatgca tcgaatagaa gttcgaagca
    18241 aagaagcaga ttctcatctc ggacatgtgt ttaccgatgg accagtagat caaggtggtc
    18301 tacgttactg catcaattct gcctcactga aattcattcc atatgaaaag atggatgaat
    18361 taggttatgg ggaattcaaa tcacttgtag aataatggaa atacacaaag agcggtacaa
    18421 tacggggatt tactcgatcg tccgctcttt ttttattcaa gcgattgcta tttgaaagaa
    18481 acgctcccta gactcctttt ttcacaaggt gaaatgtgct acaataaagg gaacgtatgt
    18541 gtgcaaaaga aagaggacta gaatatgtat gaatatctga caggtgtggt tacctttatc
    18601 aacccctatt acatagtagt agaaaccaat ggtatcggct accagatcgc actaggaaat
    18661 ccttaccgct acagtagcaa actaaatcaa aaaataaaaa tttatgtcca tcaggtaata
    18721 cgtgaagatg cgcatcttct gtatggattt gattcgctag aagaaaagca actgtttttg
    18781 cgattagtaa gtgtttcagg aatcggtccg aaaagtgccc ttgcaatcat ggcaagcgaa
    18841 gaccactccg gtttgattca agcagtagaa tccagtgatg tcacttattt gacgaaattt
    18901 ccaggagtag ggaaaaagac agcacaacaa atgatcttgg atctaaaagg aaaattcggt
    18961 gagctgagta tcgatacacc ttttagtcta tttgatgaag caaacacaaa agacgctaca
    19021 gctctatctg aagcaatgga agctctctcg gctcttggtt acagtgatcg agaaatcaaa
    19081 cgtgtagaaa aacaattaaa agaagtagat aatcagacaa cggatgagta tctacgccaa
    19141 gcactcaagc taatgatgaa aaaataagga ggagaccaaa tgacagatga atacttgtta
    19201 tcaccagaat ctggcgaaaa tgaactgagt ttggaaaaat ctcttcgacc acagctgctt
    19261 tctcaatata tcggacagag aaaagtcaaa caagaacttc agatttacat cgaagctgcc
    19321 agaaatcggg aagaaccttt agatcatgtt ctgttgtacg ggcctcctgg attaggaaaa
    19381 acgacgatgg ccatggtcat tgctaatgag atgaccgtaa atattcgtac gacgagtggc
    19441 cctgctatcg aacgtccggg tgacttagta gcaatcttga atgaactgga agcaggggat
    19501 gtcctgttta tcgatgagat ccatcgatta cctagagtgg tagaagagat gctttattca
    19561 gctatggaag atttctatgt agatatcatg gtaggacaag ggccaaccgc acatccggtt
    19621 catttccctc ttcctccctt tactttgatt ggcgcgacga cacgggcagg tatgctttct
    19681 gcacctttac gagatcgatt tggcatcatt tctcatatgg aatattatga cgaacaggat
    19741 ctgcaggaga tcgttttacg ttcttcggat atttttcaaa cagagatcat tgaagaaggg
    19801 gcattggaaa ttgctcgccg atcacgagga acgcctagaa tcgctaatcg cttgctcaaa
    19861 cgggttcgtg attttgcgca agtccaagga gacgggaaaa tcgatcgtcc tatagcggat
    19921 caggcactta ctttgctaca ggtcgatcag gctggattgg attatgttga ccagaaattg
    19981 ttgcgaacaa tgatcgagtt atatggcggc ggaccagtag ggttaagtac gctttcagta
    20041 aacataggag aagagcgtga gacggtagaa gatatgtatg aaccgtatct gatccaaaaa
    20101 ggatttttaa agcgtacacc tagagggcga attgctactg cttatgccta tgaacatttt
    20161 ggttacgact atcatatatg atttttgctt ttagattgga ggaaaatgaa tgagtgatac
    20221 aacacgtacg aaacaattga tcaaaaacgt attgattgat ttgtgccaaa cgcaatcttt
    20281 tcgaaaaatc agcgtccaaa aaatcgcaaa cgaagcaaaa atcaatcgac aaacatttta
    20341 ttatcatttt acagataaat acgatttgct tcgttgggtc tattgtgagg atgcattgca
    20401 ttatttagat ccacaatgtc tttcacttga taattgggaa gagcaagcat tgaaaatgct
    20461 gaaagcaatt gctgagaaga aagaatttta tgcaaatact gtgctttctg acccagaaat
    20521 tttacaaaaa gagttttctc aactgatcca aaaatcattt atcaagattt ttgaacaaat
    20581 ggatgaagaa agacaacttt ctgcaagaga caaactattt tatgctcgtt ttttttctta
    20641 cggttgcagt ggtgtattag tcaattggat cacgaatgaa tatgaagaaa caccgatgga
    20701 aatcgctatg cagcttttcc gtttagccaa ggatacggaa ttatattctt atcgtcttta
    20761 tgcaaaagaa gagttggaag aacagtaata gaaaaatttt ttgttggaaa agcaataatc
    20821 gctaaaagga gatggggaaa tgacaaaaat tttatttgtt tgtttgggaa atatttgccg
    20881 ttcaccgatg gcagaaggct tgctgagaaa acgaatagca ttagaaggaa gggaagcaga
    20941 attttttgtg gattctgctg ctacaagcac gtatgaagtt ggaaagacac cgcaccctgg
    21001 aacgaaaaaa atattaaacc aagaaaaagt agatatgacg aatatggttg ctcggcaaat
    21061 cacaccccac gattttgaaa cttttgattg gatcattggc atggatcaag aaaatgtaga
    21121 agaactgaaa cgaagagcac cacgctcagc gcaggggaaa atccatttgt ttttaagtag
    21181 cattcctgga aaagaaaaag aaaatgttcc cgacccatac tataccaata actttgatga
    21241 gacatatcac ttgatcacag aaggcctgaa tcattggtgg aatatttgga ttgatgaaaa
    21301 taaaataaca aacaatttgt aaagttaacg ctttcttttt aaggcgatgt tagagcattt
    21361 ccttgttttt ttgtaaaatg ttaggtaaaa taaaattaat taagtaacac tttcttagct
    21421 aaaggaggag tgcattcgat gaacgaagag acagtatcgc caatgaagaa gcttacgaag
    21481 ttagttactc atacttcgta tcaacttttt gattcttttg taaatgacac tgaagaacat
    21541 gctaagaatg tacgattgca aggaaagttt attcttgagc tgcaaaaagc tgctactgaa
    21601 catagtctag ttatcttaca agtaagagca aatcgtttga gtagcaagta tgaaacgatc
    21661 tcaggctggg tggcgacaaa aacggtcgaa aaagaccgag tgatgatccg cctacaaaac
    21721 gaagagcagc agatccggat ggttcctgta gaacaaattg aaaaaataac aatcctttca
    21781 ccagcagggg aacaggaacg gttatcaaaa taatttatac cagatagaaa agcacgtatg
    21841 agcttgtacc atcaatcatt actaatcaaa aataaccgaa tgctttaagc tgatatcagt
    21901 aaaacggaac gttttaggat gagtatcata gcatgacagc attcggttga ttagttgaat
    21961 cgattggatg gtacaagttt ttttgataaa aaaaggtcct gctagccggg gagctagcag
    22021 gataaggagt taaaaatgaa aaagtgttgt tagggttgtt tgttggtatg cttatataat
    22081 agtcattagt tgtgaatatt ttgtgacgaa agttttcgaa agttgttaag tt
//
LOCUS       DAFBQW010000039        21779 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFBQW010000039 DAFBQW010000000
VERSION     DAFBQW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.040"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..579
                     /locus_tag="LK889_001832"
     CDS             <1..579
                     /locus_tag="LK889_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5096065.1"
                     /translation="QQNGKDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAA
                     SDHYKQFSIQDPIEARLTYKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADF
                     LSNNLLDGREIQLIFTAKTPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIV
                     PKTGSTHLVTISAVSLLLLVLATFSYAVLRYI"
     gene            606..1070
                     /locus_tag="LK889_001833"
     CDS             606..1070
                     /locus_tag="LK889_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096066.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            1067..1309
                     /locus_tag="LK889_001834"
     CDS             1067..1309
                     /locus_tag="LK889_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBK5096067.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            1327..1629
                     /locus_tag="LK889_001835"
     CDS             1327..1629
                     /locus_tag="LK889_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096068.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            1699..3762
                     /locus_tag="LK889_001836"
     CDS             1699..3762
                     /locus_tag="LK889_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096069.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            3762..6371
                     /locus_tag="LK889_001837"
     CDS             3762..6371
                     /locus_tag="LK889_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBK5096070.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            6368..8740
                     /locus_tag="LK889_001838"
     CDS             6368..8740
                     /locus_tag="LK889_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096071.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEIKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQ
                     FKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQNIVTREAEP
                     KPIQNVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLRDIKQDIKP"
     gene            8790..9122
                     /locus_tag="LK889_001839"
     CDS             8790..9122
                     /locus_tag="LK889_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096072.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            9123..9716
                     /locus_tag="LK889_001840"
     CDS             9123..9716
                     /locus_tag="LK889_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096073.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9879..11573
                     /locus_tag="LK889_001841"
     CDS             9879..11573
                     /locus_tag="LK889_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK5096074.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVGYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            12261..14138
                     /gene="ltrA"
                     /locus_tag="LK889_001842"
     CDS             12261..14138
                     /gene="ltrA"
                     /locus_tag="LK889_001842"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5096075.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            14269..14646
                     /locus_tag="LK889_001843"
                     /pseudo
     CDS             14269..14646
                     /locus_tag="LK889_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            14705..14968
                     /locus_tag="LK889_001844"
     CDS             14705..14968
                     /locus_tag="LK889_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HBK5096076.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            14968..15303
                     /locus_tag="LK889_001845"
     CDS             14968..15303
                     /locus_tag="LK889_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5096077.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            15377..16579
                     /locus_tag="LK889_001846"
     CDS             15377..16579
                     /locus_tag="LK889_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK5096078.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGGE
                     FRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQ
                     EFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPL
                     PFLNSSKK"
     gene            16595..17239
                     /locus_tag="LK889_001847"
     CDS             16595..17239
                     /locus_tag="LK889_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096079.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQANIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDNHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVVDFSYFTR"
     gene            17250..18056
                     /locus_tag="LK889_001848"
     CDS             17250..18056
                     /locus_tag="LK889_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096080.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            18078..18308
                     /locus_tag="LK889_001849"
     CDS             18078..18308
                     /locus_tag="LK889_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096081.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
     gene            18777..19073
                     /locus_tag="LK889_001850"
     CDS             18777..19073
                     /locus_tag="LK889_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096082.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLIEND
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
     gene            19075..19497
                     /locus_tag="LK889_001851"
     CDS             19075..19497
                     /locus_tag="LK889_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096083.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            19516..21108
                     /locus_tag="LK889_001852"
     CDS             19516..21108
                     /locus_tag="LK889_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096084.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIEGVD
                     QMQTIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            21129..21464
                     /locus_tag="LK889_001853"
     CDS             21129..21464
                     /locus_tag="LK889_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096085.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            21610..>21779
                     /locus_tag="LK889_001854"
     CDS             21610..>21779
                     /locus_tag="LK889_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096086.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDA"
BASE COUNT         8159 a         3382 c         4497 g         5741 t
ORIGIN      
        1 caacaaaatg gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct
       61 cctttaaacg caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc
      121 gtagacgctg cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt
      181 ttgacatata aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact
      241 tctgaaggca cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct
      301 gacttcttaa gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa
      361 actccattac aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt
      421 tctaataaaa caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt
      481 cctaaaacag gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg
      541 ttagcaactt ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga
      601 ataaaatgaa aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag
      661 tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag
      721 gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga
      781 ctacctggga atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga
      841 ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg
      901 aatttataaa caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa
      961 aaaaaataat ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc
     1021 acgatggatc acaatccaat aaatataagt tacaaaagac tagcgagtga ttgctagtct
     1081 ttttatttgt cctgaaggag gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc
     1141 cgcgtctaaa atcttagaac gccgcggtgt atctgaaaca cagtggaaac aagaagtcct
     1201 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
     1261 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
     1321 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
     1381 gttgatattt ttagagcatt acaaactgcc gcaactagtt taaaaaacaa tctgatgatt
     1441 tttgctgctg ctgcggccgt tgcttccatt gttattgggg gtcttttctt cctatttgga
     1501 cgaagaggag ctgaaaatgg gaagatgcac gtaggctatg tattgatggg ggtaggagtt
     1561 attttagcag cagcttctat tgtggtttcg ttatttggat tgtttggaca aacacctcaa
     1621 ggattataag aaagtaaaaa agtaggagta agaagtagtc gatacttttt actccttttt
     1681 tatgaaagga gaatactcat gtactttacg attgcaggca ataccaaaac agataaagag
     1741 ttaataaaaa aagcaacgaa ggaagtagga aagcgtactt cttttttacc gaaaacaatt
     1801 aaatattctg tcattgaaac ggaagaacag ctacatgtgt tagggaaagt actggaacac
     1861 gaaagtcgat cttctgttaa gttgactctc acgttacatg acacagaagg aaaaatagag
     1921 aaagctggta cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttctat
     1981 cctacattga tggatttaat ccgccttgat gtgtttaaag aaacggatga aactatgagt
     2041 gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc
     2101 gaccaaagaa aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt accatgggaa
     2161 cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa
     2221 gctgatattc cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg
     2281 gaggtagatc cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat
     2341 ccagttgaag cgaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa
     2401 ccacttatca gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc aactgtattt
     2461 gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta
     2521 tttttaaatg agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag
     2581 agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc
     2641 caatcaacaa attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt
     2701 caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag
     2761 aaagcaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa
     2821 attaaagagc gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt agcaaagatc
     2881 aataatttta ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa
     2941 gaacgtatta aagaacgaaa agaagaattt taccgaggtt tgcgtacaga taagctaaag
     3001 attagcaaag aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt
     3061 atggaaaaat acgaaaagat ccagcaagaa ttacaagtgc aaattccaaa atggaaaaaa
     3121 gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa
     3181 aaagagcgtg aacaattaga aataagaaaa cgggaacagg atcttgcgga acaaaaaatg
     3241 gcgatagaaa aacaacggtt agagcaagaa aaggaaaaag aagcagctgc tcgtaaggaa
     3301 cgtcaagaac aaatggatac attgcgacaa gctcagttaa attatctagc acaacctcaa
     3361 ccaacgcctt cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa tctaactcct
     3421 tcaccacagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt
     3481 ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct
     3541 gtggccacaa cgacgtcaat caaggaagaa gcccaagaac aagcaagaaa agagtatgaa
     3601 caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagaatc aagtatggaa
     3661 aatagcaaag agagtgatcc tagtaaggct aaagaaggat caagcgattc aactgtacaa
     3721 tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa
     3781 cgagacgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc
     3841 gctatactcc ttcaggagaa gagctaccag aaaaacatga caaacgtatt tctcaaaaga
     3901 ttttttatag tggcttagct tatcttcctt taaactatgt tgttacaaga ataccagaag
     3961 catttcatcg atttgagatt attccaggaa gtttacaagg agtgaatatt tttcagttac
     4021 aagaaatgcc gcagttattc cattttgatt caactggttt ttggaatcct ttgaccccaa
     4081 caacaagttt aggcacacgt ttagttgttt ctacgatttg tgttgttcca gctgtactta
     4141 tgggattgaa aatggacatg tggtttcgtc ctctagcaga tgggcaaaaa ggagataacc
     4201 gattaaaaac gttaaaggaa atccaacaaa tttatccgca aataccagaa tcaaagggag
     4261 gttttcctgg ttatggcgga ataccaatta ctcattataa aaagtactgg tatattcaga
     4321 cggatacagt aaatacttgt attgttggta cttcccgttc agggaaagga cagatagaag
     4381 ttttagcaac cattgacaat ttgtctcgtg cagaaagaca aagttcaatg gttgttaatg
     4441 atccaaaaac agaattatat gttgctagta aagatgaact agaagctaga gggtatgatg
     4501 tgtatgcatt taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca
     4561 tcgtgaaata ctggaagcga ggagacatag acacagcgac acagttaacc aatactttca
     4621 cgaatacgat ttattatgat gccaacgctt cagacaataa atactttaac gaaaatgcgc
     4681 aaaaagctgt caatgcgctt atttttgcgt tattggaaca agcggatcag acaggggatt
     4741 actccaaatt aacaccgaat aatctcgtcg aacttctttc tgagattggt ggttttaatt
     4801 accaagatcc tgataatgaa ttcaagcagc tcaatgcttt agatgaattt atgaatcagt
     4861 taccacctgg aaacatagca aaaaaacaat atggggcaac aaaaattggt tctgataaag
     4921 cgaaaggcaa cattctatcg acagcgatca caggactaaa tccatttaca ctgacgaaaa
     4981 ttgcgaaaat gacttctcaa tcaaccattg attataagtc cattggtttt ccgaagtatt
     5041 tggatctcaa actacacgag agtctgttaa accagcgaat tgttgtagag ttttatcatc
     5101 aaggaaaaaa atttcatgag gaacgtgtca aagtaggcta tagaggtttt tgtgaaatta
     5161 actttgattg tcatttagaa cagggagatc atgtttgctt aaaatacgat catcgaggaa
     5221 aagaatatgt tgcttggtat gagttttctc aaagagtatt gaaagacgaa gagggaaata
     5281 tcgtttatcg aaaaaaacga ggagaaaccc atttgccaga attagaaaaa gaagccgttc
     5341 taacattaga tgaggataaa agtaacttat tagtagagtc tattcggatg cactataccg
     5401 ataaaccaat ggctattttt atgctcacac ctgattatga tccatctaac catatcattg
     5461 ttagtatttt tcttagtcag ttgtataaag aattatctac gcaatgtgtt aagactaaag
     5521 gagataaatg tcaccgtagg attcatttta ttttagatga atttgggaat atgccaccaa
     5581 tggataatat ggaaggaatc atgacagtca cagctggtag aaacatgttg tgggatttgt
     5641 ttatccaatc ctatcagcaa ctttatgcga agtatgggga acaaaacgca gcgattatca
     5701 aagaaaattg tcagacacat atttatatta tgtcgacaga cgataatacc atagaagaat
     5761 tttccaaaaa agtggggaat aagacggttg aacaagagaa cagtaccatc aattcattag
     5821 gtatgaatac tcatatcaat cgcaatgttg attcagatcg catcttaact cctgaaagaa
     5881 tttctacttt tttagaaggt gaaacgattg tactcgctcc tttaaaacga caagatttga
     5941 aaggtcataa aatacggccg tatcctattt ttaatcatgg ggaaacgaat cttccttatg
     6001 cttatcaata tattgaagat ttcaatccat cgtgggatat caatgatata gacatacatt
     6061 gtacacatgc ggacttggat cttaaggctt tacaaattga ttacgattat tttctacctg
     6121 atatctatgc acgtcgactt tatcacaata tgcacgatca aatagaagag gaagctaaag
     6181 aaaacatttt tattaaaatc gttcaatctt tagacaactc aacggcacaa aagttactta
     6241 ttcaatttta tgaagagaag caaatagatc gtttgcaaga ataccttgaa aaattgcgtc
     6301 aaaatgaaga aattacagag gttataagga aacgactttt gctagaagct gaaaaggcgg
     6361 tgaaagaatg atcattggag gaatattttc cggcattgcc gactggtggt caggcttatt
     6421 tggggatggg gatgcagatc ccaaaaccgt aacgaaagtc ttacaagatt atggtaatta
     6481 tctgcagtat caagatgtat tcagttggat ttgtaatact tttttatggg gtttgattaa
     6541 agggttatat aatctcaata ctttactaga aaaaacaatt tatcaaagct ttgatttaaa
     6601 agacttattg aacgccgctg gtgttaatga actgtttcaa agtttgatta gtaaggtatt
     6661 ggctcttttt tgtgtcgtca cattaattta tttaggctta aaatttttta tgagtaaaca
     6721 tccaccaaaa atcaaaaata tcttggttaa tctatttatg gccatgattt tgatttttgg
     6781 tggtagttct ttaatcgatg aagggctaaa tattagtaaa aacttttacg gagacgtgac
     6841 tgcagcaaat aaagcagata aaactggttc acctgctttt caaattattc aaaataatgt
     6901 gattgatgtg agtacgttac tagaaaatga tccgaacaaa gtagaacaca taccaacaga
     6961 caaaagaaat gctctcacgg cgaaaaattt taaaacagcg aatatcaatg ggattattac
     7021 acctgatcaa gctaagaaaa tgtctgaatc aagtgatatt agtacagtac aaaaagatcg
     7081 gttaaaggca ttacagtatc gactagagtt agacgataat gggaaagaaa tccctgttca
     7141 aattagtgat gaaggtttag caaaatatgt gtatacttca ggctatcgcc gatacattag
     7201 tagtccaggt attattttag ctggtgaatc ttctttgtcg gtagcctatt tatttatctt
     7261 gtttacgatt gttacttgca ttattgagct ggtatttaaa aaattctatt tagtgattgc
     7321 cgcgtcaacg gactttgaga caggacaacg gatgaaaagt gctatagaag atatttctca
     7381 atccttcttg ttattggcat ttacgatttt agaattacga atctatacat taatgctttc
     7441 aggtatcggt gatttgcatg cggcaggaaa gatcaatgga ttcttgtatg ttgtcgcttt
     7501 aattgtatta accattgcgc tattcaaagg ctctcaatca gttacgaaaa ttttcggcgt
     7561 tgatacgagt ctgaaaaacg gcagcaattc tcttcttagc atgtttgcct taggaaatgt
     7621 tgcgaaaaat gtgggtagcg gggctaaaaa ccttgcaagt gctggtaaga gtggtttaag
     7681 caatttaaat gatattcgta aaaatggatt tggtaatcga aaagatgtta cccctgataa
     7741 cggggaagaa caatcagacg ataaagctac aactgtacct aatagtaaga aaacagtttc
     7801 cgaaatgttc gggaaagcga aaaatggtgt ttctaaagcc gcagaaggtc ttggttacgt
     7861 caatgaacgt gggttaaaag gaacagctga agatcttaca gaaaaagcca aagacacctt
     7921 agaagagaaa ttagacggca cgagagctaa agaattggct gaagctgtga agaatccatc
     7981 cgaaacacta gaaaaacaaa aagaaaaagc ttcagaaatc aaagaaggac taaaagatgc
     8041 gtatggtgaa ggacaagtca atgcagcagt aaaagctaat acaacaaccg aaaaagaaaa
     8101 tcaagaaggt gcagcgattc cttcagccaa agatcaccaa gatgagttgc attctaacga
     8161 aaattcagaa gaaggacaaa atggtcaatt accaaaaaca gatccagaac aatatcgtta
     8221 taaatctaac attaattctg aaaatgaaaa gcgcatttca gatttaccaa aacgaaatca
     8281 agaagtacaa acggatgatg attccatcga tctgccaaat gataatggca tcgaagttgg
     8341 aactgaaaaa tcaacgaaaa ttggtttgcc taagacacaa aatttaccaa tgaaagatag
     8401 tcgtttgaat caattcaaag agaatgatca aagctttgtt catcaatcac ctgcaggtac
     8461 tgttcaaatg aaatcaagtc gtttgcctga aaatgggaat ttgacaactg tttcttacaa
     8521 tttatctgaa agtggtgaga cagagaatgc gtccgttcaa aatattgtga caagagaagc
     8581 tgaaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg tggcatttac
     8641 tgcaccgaca aaaataaatg tgacaagaga taccaaaaac aggaacaatg cattgaaaga
     8701 acaactattg agggatatca aacaggatat caaaccataa aaggcctaca agttaggcct
     8761 tttctttata tgaaaaggag aaatcaatta tggataaata tataaacatg aaaaaccctc
     8821 caagatttag tggagaatgg aagtttttcg cttttttctt tttagatcta ttaacgcttg
     8881 gtggctgctt tttttgggcg aaaatggtca ataatcttgt ccacttacca ctcttttttg
     8941 cactcttgaa ttatggctta tggattgcga tagcgttagt gattgtttgg cgaccctata
     9001 ataacccagg tcaacgacaa atttttgcga ttatcgagtc tatttttttt gcggacaaga
     9061 accattatca tgcgttagat cctaaccgaa attatcatgg tacgtataag gaggatgaat
     9121 aaatgcgagt caaaggacaa aattcagttg ataagaaatc tattttgcag tttggaaaac
     9181 cagaagaacg atctatcttg gatttatttc cttataaatc ctgtacagaa gagggagtct
     9241 tagttactaa agaagatcat tttcaacgtt attatcgtgt cgtttccact gacgtggagg
     9301 gattaaatga acaagagaaa ctggaacgaa tgaatcagtt gaccaccgtc atgcgtacgt
     9361 atgtgccaac aattaagtta gttagtttga ctacagagac ggatctaacg gaacaaatcg
     9421 tgcaaaagcg gcagttatta gataagaatc gtatggaaca atccttagga agaaatctac
     9481 aacgattaaa aaaatatgaa aagaaattag tcgaagaaat ccaggaattg aaacgagcag
     9541 aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca atcaaggatt
     9601 tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat tttagggtta aaaccactct
     9661 acaaaaaaga attgatcaag attctgtatc gaatgaacaa tatgaatgat gagtaggaat
     9721 ggagaagaga gaatgaaata tccattgata tggttagaaa aagatgttcc gaaggaagag
     9781 accactagaa aatttcctca cactttaagc ctgggtaagt tgggaaaagg gcaatctttt
     9841 aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca attaaaacag
     9901 ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta ggaaatcggg
     9961 aaatagaaat tttgaaatcc aaagggtatg atttagattt tttaaaacaa gtgatgccac
    10021 aaggtgggtt aaattttgat gaagaatatg tgacgtatgg taatggttat tcacgatgtg
    10081 tgacagttta tcactacaca aaaaatccta cgcttttttg gttggccaat ctcatgaaca
    10141 atcctcatac agtggccaca ctcgatgtta gcacagatga aaagcaaaaa gtgattgatc
    10201 aaatcaatcg tgccttagat gaattaaatg atcgatcaga taatgaacga aaagcgactg
    10261 accgaaataa aagcgcagaa gaatacctgg aactagcgga atatgctcgt aaattgactt
    10321 ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga aacacgattc
    10381 aagaagtaga tgaacgagcg caggaactga ttgaaaattt aaaagggatg gattatcgtg
    10441 cagtgaccta tctgcacaaa acgaaaagag attggcaatc gatgtttacc atgttcgatg
    10501 aacaagctga atggtttgga aatatcaaag ggcaatcact accatcgtta aatattggcg
    10561 gaggattccc gttccaccac caaacgctaa aagatccagg agggactcaa tatggggaaa
    10621 ccatgacagg tggcccattt gtctggaatg cgacatataa aaatagtgtg cggttgtcta
    10681 ctaatacctt gcttctagga atgatgggag ctggtaaatc aaccgttctt aaaatgatcg
    10741 cggaagccca tttagctgca ggagacttta tttggggctt tgaaaagggg aaagacttta
    10801 tccccttctt aaaggaatat aacggcatta tggtgcgctt agacgggtct gacgggatga
    10861 tcaatccttt agaaatattt gccacacgga cgtatgatga agcatccagt ttatatgacg
    10921 atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaaca cacttagaca
    10981 aagtaggcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat
    11041 ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgc ggctttactt
    11101 cctcaaattc gcgttcaaat actgagacgc aagtcacagg gaaagatcca gaagcttatc
    11161 cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt gcttcccaag
    11221 agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt gagacgtatg
    11281 gcatgatttt tgatggtcac agtacgattc gacaccttga ccaacaacag ttggtctttt
    11341 ttgacattga ctctattaca ggcttagatc gaggggtaca acaatgtcaa atgtacatgg
    11401 ctatgacgtt gatttggaat caggcattga ttcaaggaag aagacaacga cgtttactta
    11461 aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt gatgagtgcc
    11521 ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc taaaaactat
    11581 tgtaataact cttataacga gaagtcaaat gagtgggtaa cgccacaaag ggcttccact
    11641 gatactccga catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag ctcggtgaag
    11701 tcggctgaag gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt tctttagagg
    11761 taaagggtgt atccgataag tcgggtgtcc aaaaaaactt aatatggtga gtatgtacaa
    11821 acgtactgac gaactagcga atgtactgtc cgaaaggcat agctcctaga aatgggagag
    11881 acagtgagca aaaaatcact cattgtgcta acttgtggtt gagtaaaagt cgttgttatg
    11941 aaagaccatg ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta gcatctaagt
    12001 taaggcatgt ggatagatat aagggaacag gggaagccat caacatggag agtgcatact
    12061 cgatgaagtg gtagtaagaa atatgtgaat gacaaataac ttactttagt gatggtgaaa
    12121 gtagagtcac gactgatgaa ggttctgtaa tggaactgga ggaacaggct cgattcgtct
    12181 gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa ggtcacgagc
    12241 caattggagc gtgataaacc atgacaaaag aaacaagtcc aaaagatagg aagttgcggc
    12301 atgcagaata ttatggcatg acggaaatct tcgataagat atactccaaa agtttgaagg
    12361 gagataattt caagaatcta atgcagatta tcatttctga taacaacatt gtcctagcat
    12421 atcgtaatat caagagaaat cacggtagtg tgacaagagg agaagatgga gtaacgatta
    12481 aagatattga aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag cgattcgcac
    12541 attataaccc tcggaaagta aggcgtgtag aaattcctaa atataatggt aaattaagac
    12601 cgttgggtat tccatcaata tgggatagaa tcgcgcagca atgcatctta caagtattag
    12661 aaccaatatg tgaagcaaaa ttcaataatc acagctatgg tttccgccca aatagatcag
    12721 cagaacacgc aatagcagat tgtatggccc gaatgaatca aagtcacatg gaatatgtgg
    12781 tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca tactaaactc atgagacaaa
    12841 tctggtctat gggtatacga gacaaacagc ttctcttaat cattaggaaa atgttaagag
    12901 cacccgtagt acttccaaat gggaagttga tatacccaac aaaaggaaca ccacaaggcg
    12961 gaatacttag cccattactt gcgaacatca atctcaacga atttgactgg tggatagcaa
    13021 atcagtggga ggaaaaagat gccaaagagc taaaacatca ttttaggaaa aatggagctt
    13081 gggataaagg taatatctat cacaagctca gaaagagtac aactctaaaa gaaatatacc
    13141 tagtgagata cgcagatgat tttaaaattt tctgtagaaa ttataatgaa gcgaaaaaag
    13201 ttttctatgc aactaaaatg tggttacagg agcgtctaaa gttaccaata tctgaagaaa
    13261 agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt acgatgaaat
    13321 tagtgaccaa aggtaacaaa aaggttgtta atacgcatat aggtacaaaa gcacaaagca
    13381 tgattaaagt gaaactaaaa aaacaaatca aacgaattca aaaaagtcct aatagcaaag
    13441 atacctttat ctatattgga gtctataaca gtatggttat tgggattcat aattacttca
    13501 aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc aagagaactc agaccaagtt
    13561 ttgaaatacg actacgagaa caaggaatct caaaagaagg acaatacgga aagtacgaag
    13621 gatacaagcc ttacctacgg tcaggacaaa tacgatatat aagagggttt ccaatattac
    13681 cagtagggta tattcaacac agacatccga tgtgtaaaaa aagaaatatt aataaataca
    13741 ccgaagaagg tagggaagcc attcataaga agcaacaaac cgtagaagaa tggaagatat
    13801 cgtggttgtg tagcaatcca gtgcttagta agagagcaac gattgaattt gcggacaatc
    13861 gaatttcaaa attcatcgct caaaagggaa gatgtgcagt aacaggtgag gagcttatct
    13921 tatctgaaat gcattgtcat catattatcc cttatcatga aagcaaaagt gatagctatg
    13981 aaaacctagt gattgttact gaggaggttc atcgggtaat acacgctaca caaagtgaaa
    14041 cgattgagga acttctaaaa tatctaaaat taaatccaaa acaaaaagaa aaactcaatg
    14101 agttgagatt aaaagttggt aacgaagaaa tatcttaata aatatttgta ggacgggaaa
    14161 acagaatgtc ttttatagac gagggaacgc cgtatgcggt gaaagtcgca cgtacggtgt
    14221 ggactggggg aaaagacgga gataatatca aactcttacc tatcagtatc gattaatcca
    14281 gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg taaagtagaa
    14341 gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa tatgtcttct
    14401 gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat ctttatgcgt
    14461 atggatgaat ctattttacg tcatatagaa ggattagttg gtaaatcaat gacgaactca
    14521 gaactagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt tggctcaaaa
    14581 gaaaccattc gtgttcattt tagtccgaac aaacgtcaat tgaaattgtt tgatggtgga
    14641 aagtaacttc ttgtattttg gtgtatacaa gtatatacta atgtgtgaga gaaggaggga
    14701 ttccatgtta gtaaaagaaa tcgaaatgaa aaaatgggga aattcacaag gtattcgttt
    14761 tggaaaagaa gaattgtcag ttctaggaga aactaatact gaaaatttaa aattcgaaat
    14821 ggtggtaaca gaaggaaaaa ttgttttaac tcctaaaaat aaaaagccag aaactttaga
    14881 tgaattgttc gccaattatg aaggagaacc cttaggaaaa gaagacaaat atgaatgggg
    14941 aaatatgcaa ggtagggaga tcatttaatg aaaaagaact atcatgattt atcgcaagga
    15001 gatcttgttt atattgattt aaatcctacc aaaggacatg aacaacgggg aaaacgacca
    15061 tgtattattt tatctaaacc taataaatat ttgaactata tgattggtgt cgcaccgata
    15121 acaacaaaag cgaagccttt tcctgtacat gttgatttga atggtacgca aaaaataaaa
    15181 ggacaaattc ttttggaaca ccatcgaatg attgacattg agagtagagg ttttcagttt
    15241 gtggaagctg tgccaaagga aattttagaa gaatgtattg aaaaaattaa attattttat
    15301 taaaagcagc gaagaataat tattatttct tcgctttttt gttgtttaaa attaaaaagt
    15361 taggagtact gactatatgg aaaatgatac aaatatagtt atggaacatt tgaagcgtcg
    15421 tgcaaagcga cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt
    15481 gatcactgtg ttcgtaaccc tttttctaat acttgcggcg ggttcgattg ataattcgga
    15541 ttccgattca agtagtggtg gcgaagcttt tacaggagag tattcggaag gcttaccgat
    15601 ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgccgtagg
    15661 tgcagcagtg aaatataaat tattaccaag tgtgattctt tcgcaatacg gttatgaatc
    15721 agcgtttggt acgtcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt
    15781 tgatggttgt ctgtttccta aaggaactgc acgaggaatt ggtggcattg aagggggctg
    15841 gtacatgaag tttcccaact caaaagctgc attctcctat tacggcttta tggtagccac
    15901 acaatcaaat tttaatgcat gtgtaggaaa taaaagccct ggcgcaagct tattgatctt
    15961 aggacgaggt ggttatgccg ctgcaggaat tactgaagat agtccgtatt atacaggttg
    16021 tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcca tcaaacattg
    16081 gggtgaaggt ggcaataaca atggcacaat tactggtgaa tggacgaatc cattccctgg
    16141 cagcagttta gataaaagtt ccttcagcgg tggtcaatta tttggaacga atccaggcgg
    16201 tgaattccgt ccgaacggct tccatgatgg attagatttt ggttcagttg atcatccagg
    16261 aagtgagatt catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc
    16321 aggtttgggc gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga
    16381 atttgcaaat agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca
    16441 agtaattggg atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg
    16501 tcaagcacaa ggacatgcat tcattgatga tggtacatgg atcgatccgt taccattttt
    16561 gaatagcagt aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt
    16621 cttaacagct gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa
    16681 agaggaacaa gcagcaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa
    16741 gctcaaaaaa aattcctctg aggcacaggc gaacatcgac aagttaaatc aaaccatcaa
    16801 tgagaaaaac caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga
    16861 agtaactgaa gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc
    16921 attaagcaga aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga
    16981 caaattaagt cctgcagacg ataatcacca acatgatgat tacggaagtt actcctttac
    17041 attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt
    17101 cgtatttgtt gattatacaa tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat
    17161 aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcgtagactt
    17221 tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt
    17281 tcaaaaaacg aaaaagttat cttcaatggc tgggaatctt tatgattctc ggctttttga
    17341 ttcttgctag tccgatttat acgaatcaga aggtagatat accagatgag gtaagcacga
    17401 acgccttttt aaacacgaaa gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc
    17461 aattagtttt tgaatttgcg atcgacgatg aagatcaaag cttgttaaaa gaactggcta
    17521 atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca atacgagcca
    17581 aagtgatcaa ggtgtctgat gattattttg cagtgaagct aacgcatgta ccagaagaat
    17641 atatcgccat gcgtttaacc attattcctg aaaaaattga tccaaaagtg gatatgcaag
    17701 aaccacaaga tctgatttat tacgtgcacg aagataaggt taaagatcaa gtgaaagatg
    17761 aagattatga acaacacgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag
    17821 cagctcaaaa acaaatggaa tctttccaag caaccattga tttgaatgaa gaattagtga
    17881 agaagctaaa agatcaattg ccttatcaag tcgcagatga tcaggaaaat actgaaaaca
    17941 agattaatag ctaccagcaa gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag
    18001 agatcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaag atttagaagg
    18061 acaggtggag tgaaaaaatg aagcgaacaa acttggctga caaagatact ttcgctgagc
    18121 aaaaagtatt gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag
    18181 tgaatttatc taacacaaca atatctgagt tgaagaatgg tcgaaagaat ttagatactt
    18241 taaaattcag atcgtttgaa gctctatatg tagctgctaa aaaatatttt acggctgaaa
    18301 ggaaataaaa acaggaggat tttatgtgaa aaaagtatat caaaaatgtt tatggatcag
    18361 ctatatcacc gtaacacaaa catcaagcac ataaaacatt taacactcct cctttctggc
    18421 tgaagatttc aaccaaacag gaagacagcg tatcaattat agcatacact ttttcttaaa
    18481 ttcatttttc atatgacaaa atatttcctt ctaatcgaca aagttagaag gattttttat
    18541 tgtctaaata cacaaataac cttaattttt aaagagcata aggaaacgtg accccacttt
    18601 taaaaaaaag tggttggata ggctgtgcct atcacgtttc tgaaaaaaat aaatgacccc
    18661 aaaataccaa tatataggat agtacaagga tagcaaagga tagtaaaagg ataggggtag
    18721 gatagcgaaa aggataggct ccttttcact atcctatatt atattggagg atatttatga
    18781 atgttaaaat tagagattta gatccaaaat tcatttctga aattgatcga agatgcgttg
    18841 aactaagtaa tcgtacagga aataaatgga gtcgtaacga ttatttaaaa ttactaatag
    18901 aaaatgattt tgatcgtcct ttgatggaat ataaaaaaga aaagtttgat cagttattag
    18961 aaaagttttc agaaattcaa agctataaca caaagatatt agaagaatat gttagccaaa
    19021 acaatcgtat tttagaaatc ttgatcgagc aaaatagagg aagtgaactt tgatatggca
    19081 aaggttatag acaaacacat ttactttcac caaacacata tcgatgtatt ggatgaaatc
    19141 atttctactc atgcagaggt taaaaactat agtgaagccg ttcggtttct atgtatgaac
    19201 tatgatccag aagtggagaa aaaagtaaat tccaatcaag caaagttgaa tggaatgagt
    19261 aaagaaattt ctattttaac cgaaatggtt tcagagtttg cagatcttca tttgaaagat
    19321 acaggaatac taattggtac aaattcaacg gtttaccaag aagcaaaaga acgtgtggaa
    19381 gggaaaatta aatctaatca aactaaaaaa tattcccctc agttgaagaa acctgtgaat
    19441 gaatctgttc atgccagagt agatccattt gatccaacga agattctaaa aagataaaag
    19501 tagaaggaga acgtgatgaa taaatcgcct gcagtcattc tcacttgtca atttaccttg
    19561 cccaataaaa aatctttttc aacatacatt gattatatga caagagaaaa agcgctggaa
    19621 gaaaaggaac gacgcacgcc cgacgaagaa caggaactgc aacgaatcaa gaatgcttta
    19681 gaggattttt atattcctga aggagaaact ttttcggaga gtaagacgga taaagaattc
    19741 agcgaaacgt cgaatgaagc tcaatctttg atgggaaatg gaatcaactt tgacaagtta
    19801 gaggaacaag attttaccaa atatctttct tatatgaccc gtcactatgc gttagaacaa
    19861 aagaaagaac tgacgcaaag tgaacaaaaa gaataccaac gcttgaacca agcaatgaaa
    19921 aaatatcaaa cagaacccca taaaaaagct tcaggaaatc tccctggagt tttttctatg
    19981 gcttcagacg aagtgaaagg aagtgatctg aaggagatta agcaaatctt ccaaagagga
    20041 caacaaaaag gctcaatcat ttatcaagat gttgtttctc atgataattc gtatcttgaa
    20101 aaaataggat tgtacgatcc caaaacagat actttagatg aagaaggatt aaaagcagct
    20161 ggaagaaaaa tgatggagac attattcgag aaagagcaac tgaacgaaac aggctactgg
    20221 atggcaacga ttcaccgtaa taccaaacat atccatattc attttgcagt tgttgaaaaa
    20281 gaaaatacgc gtaaacctta cacggaggtt gaaaatggtg tgagttatat cgtaccgaaa
    20341 ggtaagagaa gacaagcaac attagacgca atgaagacca attatgtaca tgagttagaa
    20401 cgtttttctt atgaaaagga tcgaatgatt ggtatggaaa aacgaaattt attgactcgg
    20461 aaaagtgatt tgcgtaacac cttaacaaaa gtggtaaaag agccagcgcg ttacgatcga
    20521 cacgcaatga acttattaaa tgaagtatat aaaaccctac ctgacaaacg ttcggaatgg
    20581 aattacgggg atgaaaaacg aaccaaactt tcaccataca caagagagaa actcgatgag
    20641 ttaaccaaat atgttttaac aaaagaacct gaataccaag aatatataca gatttcaaaa
    20701 gcattgaaag aagaagcaaa aagtctttat ggggaaagca agagagaatc aaagaatgca
    20761 gaaaaaaacg ccctctttga tttaaaaaag cgcacaggaa atgccatcct aacagaactg
    20821 aagagacaag atagtcaaat aaaaccatta atagaaggtg tcgatcagat gcaaactatt
    20881 tacaatgaac gatcctttcg aacacttaaa cagttgccaa caaatgaata caagtatagt
    20941 tacgaagaat tcatgaagcg aaggaaacaa ttccaacaaa gagttgtcac gcaacggagt
    21001 ttgcgtcaat taaatcgaac gattaatgcc catgaagaaa aataccacgc aagtaaagca
    21061 tacgaagagc aacaacgaag aattcaaatg gaacaagaaa gacagtaaag acagtaggag
    21121 gaatagcaat gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag
    21181 aacgaatcga acaacttcct gattgtttag aaaaagagca tttgttccag ataagagaaa
    21241 tgttgagaaa cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg
    21301 gtgtgtattg ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg
    21361 gcaagttagc accttattat actacttggg aaatgaatga agagggaatc aataactttc
    21421 catgtaaaag cattgaagag tccatttcac tcacagagtg ttagttgtta agtgaataaa
    21481 agaagtaaaa gagtcctgaa cgtgattgtc caggactctt tttttcaatg aattgttttc
    21541 cgccaactca ttgagtggtg cgatttgcga ctacattata tcaaaagaaa gagaagggag
    21601 gaagcagaaa tggttcattc aacgattgga gttgttgttg aaaaaagcag agacaatctc
    21661 gtttttgtga cagaaattca aacaggcaga tcttttgtcg tcacagacaa agcggcaaaa
    21721 gcttatcaaa gtagcgatat tttaacgtta aatctgacta cgaagacgtt cgttgatgc
//
LOCUS       DAFBQW010000040        20941 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFBQW010000040 DAFBQW010000000
VERSION     DAFBQW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.041"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            168..362
                     /locus_tag="LK889_001855"
     CDS             168..362
                     /locus_tag="LK889_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096087.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..929
                     /locus_tag="LK889_001856"
                     /pseudo
     CDS             631..929
                     /locus_tag="LK889_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1026..1460)
                     /locus_tag="LK889_001857"
     CDS             complement(1026..1460)
                     /locus_tag="LK889_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5096088.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="LK889_001858"
     CDS             complement(1692..2402)
                     /locus_tag="LK889_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK5096089.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="LK889_001859"
     CDS             complement(2392..3252)
                     /locus_tag="LK889_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK5096090.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="LK889_001860"
     CDS             complement(3257..3895)
                     /locus_tag="LK889_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK5096091.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="LK889_001861"
     CDS             complement(3910..4209)
                     /locus_tag="LK889_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5096092.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="LK889_001862"
     CDS             complement(4252..5736)
                     /locus_tag="LK889_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK5096093.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="LK889_001863"
     CDS             complement(5756..6217)
                     /locus_tag="LK889_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5096094.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="LK889_001864"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="LK889_001864"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK5096095.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="LK889_001865"
     CDS             7520..8290
                     /locus_tag="LK889_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5096096.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="LK889_001866"
     CDS             complement(8563..9390)
                     /locus_tag="LK889_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5096097.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="LK889_001867"
     CDS             9958..11193
                     /locus_tag="LK889_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK5096098.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="LK889_001868"
     CDS             complement(12192..12452)
                     /locus_tag="LK889_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK5096099.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="LK889_001869"
     CDS             complement(12479..13780)
                     /locus_tag="LK889_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5096100.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="LK889_001870"
     CDS             complement(14072..14530)
                     /locus_tag="LK889_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5096101.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15874)
                     /locus_tag="LK889_001871"
     CDS             complement(14543..15874)
                     /locus_tag="LK889_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBK5096102.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17327)
                     /locus_tag="LK889_001872"
     CDS             complement(15885..17327)
                     /locus_tag="LK889_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5096103.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="LK889_001873"
     CDS             complement(17314..18078)
                     /locus_tag="LK889_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBK5096104.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18182..19057)
                     /locus_tag="LK889_001874"
     CDS             complement(18182..19057)
                     /locus_tag="LK889_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5096105.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19312..19396)
                     /locus_tag="LK889_001875"
     tRNA            complement(19312..19396)
                     /locus_tag="LK889_001875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19360..19362),aa:Leu,seq:caa)
     gene            complement(19414..19485)
                     /locus_tag="LK889_001876"
     tRNA            complement(19414..19485)
                     /locus_tag="LK889_001876"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19451..19453),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="LK889_001877"
     tRNA            complement(19495..19567)
                     /locus_tag="LK889_001877"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19629..19713)
                     /locus_tag="LK889_001878"
     tRNA            complement(19629..19713)
                     /locus_tag="LK889_001878"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Leu,seq:caa)
     gene            complement(19734..19804)
                     /locus_tag="LK889_001879"
     tRNA            complement(19734..19804)
                     /locus_tag="LK889_001879"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19770..19772),aa:Cys,seq:gca)
     gene            complement(19850..19921)
                     /locus_tag="LK889_001880"
     tRNA            complement(19850..19921)
                     /locus_tag="LK889_001880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19887..19889),aa:Gln,seq:ttg)
     gene            complement(19933..20005)
                     /locus_tag="LK889_001881"
     tRNA            complement(19933..20005)
                     /locus_tag="LK889_001881"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19970..19972),aa:His,seq:gtg)
     gene            complement(20008..20081)
                     /locus_tag="LK889_001882"
     tRNA            complement(20008..20081)
                     /locus_tag="LK889_001882"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20047..20049),aa:Trp,seq:cca)
     gene            complement(20088..20168)
                     /locus_tag="LK889_001883"
     tRNA            complement(20088..20168)
                     /locus_tag="LK889_001883"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20132..20134),aa:Tyr,seq:gta)
     gene            complement(20170..20245)
                     /locus_tag="LK889_001884"
     tRNA            complement(20170..20245)
                     /locus_tag="LK889_001884"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20210..20212),aa:Phe,seq:gaa)
     gene            complement(20281..20353)
                     /locus_tag="LK889_001885"
     tRNA            complement(20281..20353)
                     /locus_tag="LK889_001885"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20318..20320),aa:Asp,seq:gtc)
     gene            complement(20363..20435)
                     /locus_tag="LK889_001886"
     tRNA            complement(20363..20435)
                     /locus_tag="LK889_001886"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20400..20402),aa:Val,seq:tac)
     gene            complement(20451..20522)
                     /locus_tag="LK889_001887"
     tRNA            complement(20451..20522)
                     /locus_tag="LK889_001887"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20487..20489),aa:Glu,seq:ttc)
     gene            complement(20537..20626)
                     /locus_tag="LK889_001888"
     tRNA            complement(20537..20626)
                     /locus_tag="LK889_001888"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20590..20592),aa:Ser,seq:gga)
     gene            complement(20666..20739)
                     /locus_tag="LK889_001889"
     tRNA            complement(20666..20739)
                     /locus_tag="LK889_001889"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20703..20705),aa:Asn,seq:gtt)
     gene            complement(20749..20864)
                     /gene="rrf"
                     /locus_tag="LK889_001890"
     rRNA            complement(20749..20864)
                     /gene="rrf"
                     /locus_tag="LK889_001890"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6601 a         4390 c         3332 g         6618 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcgtcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaaa ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
    18181 tttaatagat tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
    18241 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
    18301 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
    18361 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
    18421 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
    18481 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
    18541 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
    18601 cttctactgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
    18661 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
    18721 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
    18781 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
    18841 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac
    18901 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
    18961 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc
    19021 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
    19081 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
    19141 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
    19201 ttttttactc gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa
    19261 acaaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
    19321 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
    19381 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
    19441 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
    19621 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
    19681 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
    19741 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
    19801 cgccattata ctattatcaa tctataaaat aaagaataaa ttatagaaac tggggtagct
    19861 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
    19921 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
    19981 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
    20041 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
    20101 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
    20161 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
    20221 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
    20281 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
    20341 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
    20401 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
    20461 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
    20521 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
    20581 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
    20641 tcaatactct ttctcaaaat gagaactccg gcagtaggac tcgaacctac gacatcatga
    20701 ttaacagtca tgcgctacta ccaactgagc tatgccggaa taacatcagc gtggcgacgt
    20761 cctactctca caaggggcaa cccctcacta caatcggcgc taagaagctt aacttctgtg
    20821 ttcggcatgg ttacaggtgt atccttctcg ctatcgccac cacactgtgg tgttatcttt
    20881 tattgagtaa attttgttca ctcaaaactg gatttgaagt cacataagtt tttttccgag
    20941 t
//
LOCUS       DAFBQW010000041        20782 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFBQW010000041 DAFBQW010000000
VERSION     DAFBQW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.042"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(18..1574)
                     /gene="rny"
                     /locus_tag="LK889_001891"
     CDS             complement(18..1574)
                     /gene="rny"
                     /locus_tag="LK889_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBK5096106.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1985..3034)
                     /gene="recA"
                     /locus_tag="LK889_001892"
     CDS             complement(1985..3034)
                     /gene="recA"
                     /locus_tag="LK889_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK5096107.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3143..4384)
                     /locus_tag="LK889_001893"
     CDS             complement(3143..4384)
                     /locus_tag="LK889_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBK5096108.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4624..5202)
                     /gene="pgsA"
                     /locus_tag="LK889_001894"
     CDS             complement(4624..5202)
                     /gene="pgsA"
                     /locus_tag="LK889_001894"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK5096109.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5411..6334)
                     /locus_tag="LK889_001895"
     CDS             complement(5411..6334)
                     /locus_tag="LK889_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096110.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7073..8359)
                     /locus_tag="LK889_001896"
     CDS             complement(7073..8359)
                     /locus_tag="LK889_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK5096111.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8352..9620)
                     /locus_tag="LK889_001897"
     CDS             complement(8352..9620)
                     /locus_tag="LK889_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK5096112.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9872..10333
                     /gene="mscL"
                     /locus_tag="LK889_001898"
     CDS             9872..10333
                     /gene="mscL"
                     /locus_tag="LK889_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK5096113.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10392..10838)
                     /locus_tag="LK889_001899"
     CDS             complement(10392..10838)
                     /locus_tag="LK889_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5096114.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10931..11176)
                     /locus_tag="LK889_001900"
     CDS             complement(10931..11176)
                     /locus_tag="LK889_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBK5096115.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11290..11868)
                     /locus_tag="LK889_001901"
     CDS             complement(11290..11868)
                     /locus_tag="LK889_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5096116.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(11945..12475)
                     /locus_tag="LK889_001902"
     CDS             complement(11945..12475)
                     /locus_tag="LK889_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBK5096117.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12479..13339)
                     /gene="rlmB"
                     /locus_tag="LK889_001903"
     CDS             complement(12479..13339)
                     /gene="rlmB"
                     /locus_tag="LK889_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK5096118.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13332..13727)
                     /locus_tag="LK889_001904"
     CDS             complement(13332..13727)
                     /locus_tag="LK889_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBK5096119.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13724..15136)
                     /locus_tag="LK889_001905"
     CDS             complement(13724..15136)
                     /locus_tag="LK889_001905"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK5096120.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15133..15669)
                     /gene="cysE"
                     /locus_tag="LK889_001906"
     CDS             complement(15133..15669)
                     /gene="cysE"
                     /locus_tag="LK889_001906"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK5096121.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15755..15994)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15991..17451)
                     /locus_tag="LK889_001907"
     CDS             complement(15991..17451)
                     /locus_tag="LK889_001907"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK5096122.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17621..18778)
                     /locus_tag="LK889_001908"
     CDS             complement(17621..18778)
                     /locus_tag="LK889_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBK5096123.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18812..20182)
                     /gene="radA"
                     /locus_tag="LK889_001909"
     CDS             complement(18812..20182)
                     /gene="radA"
                     /locus_tag="LK889_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK5096124.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20245..20772)
                     /locus_tag="LK889_001910"
     CDS             complement(20245..20772)
                     /locus_tag="LK889_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK5096125.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         5473 a         4592 c         3706 g         7011 t
ORIGIN      
        1 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
       61 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
      121 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
      181 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
      241 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
      301 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
      361 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
      421 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
      481 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
      541 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
      601 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
      661 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
      721 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
      781 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
      841 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
      901 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
      961 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
     1021 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
     1081 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
     1141 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
     1201 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
     1261 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
     1321 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
     1381 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
     1441 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
     1501 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
     1561 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
     1621 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
     1681 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
     1741 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
     1801 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
     1861 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
     1921 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
     1981 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
     2041 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
     2101 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
     2161 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
     2221 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
     2281 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
     2341 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
     2401 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
     2461 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
     2521 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
     2581 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
     2641 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
     2701 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
     2761 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
     2821 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
     2881 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
     2941 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
     3001 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
     3061 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
     3121 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
     3181 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
     3241 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
     3301 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
     3361 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
     3421 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
     3481 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
     3541 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
     3601 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
     3661 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
     3721 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
     3781 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
     3841 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
     3901 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
     3961 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
     4021 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
     4081 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
     4141 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
     4201 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
     4261 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
     4321 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
     4381 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
     4441 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
     4501 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
     4561 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
     4621 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
     4681 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
     4741 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
     4801 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
     4861 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
     4921 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
     4981 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
     5041 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
     5101 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
     5161 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
     5221 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
     5281 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
     5341 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
     5401 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
     5461 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
     5521 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
     5581 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
     5641 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
     5701 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
     5761 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
     5821 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
     5881 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
     5941 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
     6001 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
     6061 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
     6121 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
     6181 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
     6241 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
     6301 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
     6361 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
     6421 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
     6481 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
     6541 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
     6601 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
     6661 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
     6721 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
     6781 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
     6841 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
     6901 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
     6961 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
     7021 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
     7081 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
     7141 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
     7201 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
     7261 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
     7321 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
     7381 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
     7441 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
     7501 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
     7561 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
     7621 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
     7681 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
     7741 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
     7801 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
     7861 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
     7921 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
     7981 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
     8041 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
     8101 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
     8161 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
     8221 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
     8281 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
     8341 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
     8401 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
     8461 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
     8521 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
     8581 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
     8641 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
     8701 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
     8761 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
     8821 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
     8881 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
     8941 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
     9001 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
     9061 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
     9121 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
     9181 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
     9241 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
     9301 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
     9361 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
     9421 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
     9481 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
     9541 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
     9601 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
     9661 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
     9721 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
     9781 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
     9841 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
     9901 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
     9961 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    10021 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    10081 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    10141 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    10201 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    10261 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    10321 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    10381 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    10441 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    10501 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    10561 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    10621 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    10681 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    10741 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    10801 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    10861 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    10921 cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat
    10981 ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga
    11041 atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg
    11101 tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag
    11161 ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt
    11221 ttcaagttta ccataaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    11281 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    11341 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    11401 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    11461 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    11521 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    11581 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    11641 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    11701 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    11761 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    11821 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    11881 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    11941 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    12001 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    12061 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    12121 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    12181 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    12241 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    12301 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    12361 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    12421 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    12481 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    12541 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    12601 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    12661 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    12721 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    12781 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    12841 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    12901 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    12961 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    13021 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    13081 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    13141 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    13201 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    13261 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    13321 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    13381 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    13441 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    13501 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    13561 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    13621 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    13681 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    13741 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    13801 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    13861 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    13921 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    13981 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    14041 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    14101 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    14161 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    14221 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    14281 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    14341 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    14401 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    14461 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    14521 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    14581 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    14641 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    14701 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    14761 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    14821 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    14881 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    14941 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    15001 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    15061 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    15121 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    15181 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    15241 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    15301 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    15361 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    15421 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    15481 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    15541 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    15601 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    15661 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    15721 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    15781 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    15841 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    15901 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    15961 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    16021 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    16081 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    16141 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    16201 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    16261 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    16321 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    16381 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    16441 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    16501 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    16561 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    16621 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    16681 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    16741 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    16801 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    16861 attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga
    16921 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    16981 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    17041 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    17101 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    17161 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    17221 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    17281 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    17341 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    17401 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    17461 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    17521 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    17581 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    17641 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    17701 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    17761 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    17821 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    17881 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    17941 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    18001 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    18061 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    18121 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    18181 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    18241 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    18301 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    18361 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    18421 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    18481 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    18541 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    18601 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    18661 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    18721 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    18781 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    18841 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    18901 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    18961 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    19021 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    19081 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    19141 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    19201 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    19261 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    19321 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    19381 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    19441 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    19501 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    19561 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    19621 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    19681 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    19741 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    19801 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    19861 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    19921 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    19981 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    20041 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    20101 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    20161 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    20221 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    20281 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    20341 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    20401 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    20461 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    20521 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    20581 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    20641 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    20701 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    20761 tctttttctc accggttttc ct
//
LOCUS       DAFBQW010000042        20618 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFBQW010000042 DAFBQW010000000
VERSION     DAFBQW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.043"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..189
                     /locus_tag="LK889_001911"
     CDS             <1..189
                     /locus_tag="LK889_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5096126.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="LK889_001912"
     CDS             complement(1055..1585)
                     /locus_tag="LK889_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK5096127.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="LK889_001913"
     CDS             complement(1582..2199)
                     /locus_tag="LK889_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5096128.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2805..3002)
                     /locus_tag="LK889_001914"
     CDS             complement(2805..3002)
                     /locus_tag="LK889_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK5096129.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYK"
     gene            complement(3180..4712)
                     /locus_tag="LK889_001915"
     CDS             complement(3180..4712)
                     /locus_tag="LK889_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5096130.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="LK889_001916"
     CDS             complement(4848..5933)
                     /locus_tag="LK889_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5096131.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="LK889_001917"
     CDS             complement(5948..6667)
                     /locus_tag="LK889_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK5096132.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="LK889_001918"
     CDS             complement(6664..7974)
                     /locus_tag="LK889_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK5096133.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="LK889_001919"
     CDS             complement(8001..8591)
                     /locus_tag="LK889_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBK5096134.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="LK889_001920"
     CDS             complement(8909..9970)
                     /locus_tag="LK889_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5096135.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="LK889_001921"
     CDS             complement(10538..11605)
                     /locus_tag="LK889_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5096136.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="LK889_001922"
     CDS             complement(11625..12104)
                     /locus_tag="LK889_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBK5096137.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="LK889_001923"
     CDS             complement(12121..12582)
                     /locus_tag="LK889_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5096138.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="LK889_001924"
     CDS             complement(12596..13846)
                     /locus_tag="LK889_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBK5096139.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14208..15674)
                     /locus_tag="LK889_001925"
     CDS             complement(14208..15674)
                     /locus_tag="LK889_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK5096140.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGDQCRTKVL"
     gene            complement(15995..16759)
                     /locus_tag="LK889_001926"
     CDS             complement(15995..16759)
                     /locus_tag="LK889_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBK5096141.1"
                     /translation="MIDLHCHILPGIDDGAESLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16787..17485)
                     /locus_tag="LK889_001927"
     CDS             complement(16787..17485)
                     /locus_tag="LK889_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBK5096142.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17497..18276)
                     /locus_tag="LK889_001928"
     CDS             complement(17497..18276)
                     /locus_tag="LK889_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBK5096143.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(18292..19236)
                     /locus_tag="LK889_001929"
     CDS             complement(18292..19236)
                     /locus_tag="LK889_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5096144.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19282..20061)
                     /locus_tag="LK889_001930"
                     /pseudo
     CDS             complement(19282..20061)
                     /locus_tag="LK889_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20488..>20618
                     /locus_tag="LK889_001931"
     CDS             20488..>20618
                     /locus_tag="LK889_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK5096145.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTD"
BASE COUNT         6584 a         4090 c         2656 g         7288 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatctattt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatcccccct ctgaagtaca gtttttactg tcttaaataa aattttaaaa
    14281 tctaacccca tactccaatt ttttatatat tccgtgtcaa gttttgctac ttcttcaaaa
    14341 tcagtaatat cacttctacc acttacctgc cacattccag taattcctgg cttgatagca
    14401 attcttgcac gatgatgata atcatattct tcatactctg atttaaggca tggtcgaaat
    14461 cctataaggc tcatctttcc tgttagaacg ttgaaaaatt gagggaattc atctaaagac
    14521 gtggatctaa taaattgacc gattcctgtc ttttttgttc catctggaag tattttattt
    14581 ccgataattc ttggatcaaa ctccatctta aacataagat tattgctcat cttattttct
    14641 ttcattaatt ctacttttct ttcttctgca tccatataca tacttctaaa cttatacatc
    14701 ttgaacggtt taccattctg acctatacgt tcttgtgcaa agaaaattgg gcctggtgaa
    14761 ttaatataaa taagtggagc aataaagata aaaataattc ccgttgcaat acatccaaca
    14821 atacctgcaa gaatatcaat tgttcgtttt ataaatgcct gttgagttgt cataacattg
    14881 atacttgttg taagtactgt atattctcct atacgaccaa tagattgttt tcccaaagca
    14941 ctatctgata cttttgcaag attcaaatgt acagtaaggc ccatttcaga gcatcgttca
    15001 ataaattctt tcatacacga agtactttta ttcaaaataa taaagacttc atctacccat
    15061 ccctgacata agtaatccgc tgcatttttg gaatcagaaa caactgtcac tccctcaaca
    15121 tcttcaccaa tcatgctttt atctatgata atgacaccat ttattttata cattttatag
    15181 ttatgctctt tcacattacg aataactact tcagcaatac tactagatgt aactataata
    15241 agtgatttgt tcccctcatt tttcatttta tgtttcaagt aatgtttcca taagaggcga
    15301 gttatatatg taattacaac gtataaacct cccatcaaga aaagaacgac tctagaataa
    15361 tcatttccac cctgaacact aatgagatac aaacttccta acagcaccaa cataatcgct
    15421 tgctttacag aagaagcaaa ttctatatag taccctcttt ttaatacacc tctatatgct
    15481 tcaaacatga aaataactaa aagatctgcc agttcaatga agactgccat gttttgatac
    15541 attggaatca cataaggatt ccaatctacc tggcgtaaga catacgatag ataaaaagca
    15601 agctggatac acaataaatc taaaataata aaatcaatat gtttaaccca tccccttgaa
    15661 cttttcttgt acattccttt ttactcccct ctttttaata aaataaattg tattacttgt
    15721 tttctctcct aaatatcttt ctgaaggatt attgactcga caaattggct tgtggatatt
    15781 cttatatata gaacatccgt aagccaactt aaaactcctt aacaaaattt ttttactcat
    15841 attgtagatc aaaacaagta tccaagaaat catattttat ttcttttttc tgcttactca
    15901 acaacagcta aaagaaaaat catcataaac aaattcaact aagccaacga tccccttgtg
    15961 aaatcaagtt ttttcttgat ttacgaataa agagctaaaa taaaccaaac ttcttctttt
    16021 ttacctcttc atagactgga taactgattt catccccgtt gatcagatcc tttgcgactt
    16081 gtttcatctg tactactttt tccttaccaa agtcctttgc aatctgctca tagcactctt
    16141 ttaagtaaaa ggtgcgctta gagaccccat gggcatcaga agcagccatc tgcacgagat
    16201 tatgtttgac catcgttttg gctgttttct ggatgtcttt gccaaatacc ccaacataac
    16261 ttggcgcagt gagttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt
    16321 cttcgcgaaa atgcacgttg cgttctggat ggacgattac tggtgtcttt cctagttcta
    16381 acaactcaaa aaaaagccgt tctgcatata acggcacttc cagtgtcgga aattcgatca
    16441 gaagatatgt atcatctaaa tctgtaaata atatttcatc tcggtggatg tcttcgatga
    16501 gcgttccggt aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct
    16561 ctaattcggc ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt
    16621 ttccattatt gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt
    16681 cagccattgc aatacttgct tctaagcttt ctgccccgtc atcgattcct ggtaggatat
    16741 ggcaatgtag atcgatcatt tctcttgtct cctctcttct tattccttaa tttccgtagt
    16801 aataaccagc atctttgcta tgttctgctc cgttgtacac aacacctaag actcgtgctt
    16861 gtaccatctc cagcagttct tttgctttag tcaaggattc tttacgtgac atattttcac
    16921 gtacgaccaa taaagtgcca tccgttttta aagacatgat ctgtgcatct gttacggcta
    16981 ctactggtgg catatcgaat atcaccacgt caaataactg tcttgcttcg gataggatct
    17041 gatccattct tggcgagctt aacaattctg atgggttggg tggcttaggt ccactaggca
    17101 aaatcgacaa gttctcgact ggcgttcgtt ggatcacatc tgctacactt cctgatgaac
    17161 taagtgctgt acttaatcca ctggcattgt tcagctggaa tgtcttatag acgacgggtt
    17221 tacgtaaatc tgcatcgact agtagaactc gttgacctga tttagcaaaa acgactgcaa
    17281 tgtttgctgc agtggtggat ttcccttctc caggacctga tgaagtcaca acaatcgttt
    17341 tgatttgctg accagctgcg gaggcaaact ggatattcgt ccgaatcgta cgatattgtt
    17401 cagcgattgg tgaagaggga ttggtgagtg tgaccaaact gactgcatga gtttgggctg
    17461 ctttttgttt ctgtgttcgt gccattttct ttcctcctag actcttgagc ggcttctgcg
    17521 tgatggcgct gttttatcgt ttgtagcttt tggttgtgtc tgagctggtc cttttttacg
    17581 gataccagca ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc
    17641 taatgtatct gtcacatatt tttcatcttt cactgtccga tcgagtaatt ccaataagaa
    17701 tgccagacct acacctacca tcattcctaa cattaagcca gtcattaaat tcaatttatt
    17761 atttggcgaa acaggtgtcg tattggcaac tgcacctgaa atgatcgaga ttttatcaac
    17821 attcatcaca tcttttgcgt tctcttggaa aacttgtgaa gttgtgttag cgatctgttg
    17881 agcagccact gcattcgtac ttattgcctg aatcgagaac atttgtgaat tttctgcctg
    17941 attaactgag atggcatctt tgatttgttc agcagtcatt ttcacattgt cttctgtttc
    18001 taaacgatct ttgacttcat tgagcactaa atcactgacg atcatatctt tatacgtatt
    18061 gatcatttgc aggttcgtat tgacatcatt gacatttgca gtgtctgatt gtggcagtgt
    18121 cacgatcaat tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc
    18181 tgagatggcc aagccagcaa acattgagat gatgattatt accaagtgtt tgcgtaatgt
    18241 ttcaaaaatt tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt
    18301 ctctagattc atttgatctt ttaattcttt tttcacacga tcaagttctt gatcactgac
    18361 tttttgatag gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttaat
    18421 cgtaccaaat gcctcccgat aatctaaagc aatttttttc atttcatcaa agttcatgtc
    18481 tgttttcata ttcgactcta gagcattcaa aacggattga tagttgctga caccgtttag
    18541 actcaatact tttttcgcta cgccttctac gatctttctt tggcgccctt gtctaccgta
    18601 atcaccattt gggtcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa
    18661 tgtaatttta ccgattggaa agtcatagcc atcttgtgag aaagtgaggt cattattgac
    18721 ttcgatacca ccaactgcat caacgagttc ctttagccct ttcatattga tagagacata
    18781 gtgatcgatt gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc
    18841 gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt
    18901 atcacgagga atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat
    18961 catcgtatct gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa
    19021 tagaacagag aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt
    19081 tcgttccact gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc
    19141 tcctactcct acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat
    19201 ttttttccct cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg
    19261 cttgaatcaa cgttttaact gtcaatagct tatattgcta tagcccattt gctcagcaac
    19321 agtttgtgct tttgttgcgt atgctgcgct tattttttca ttccggatct gctctatttg
    19381 actgagcacc gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa
    19441 catcgtatcg aataactgat ctagttgcgt gacttgcgcg atctgaatat cagcgaatcc
    19501 tagatattgc ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg
    19561 tacacgagcg acatctgcac ttgtcacctt ttctttgatg atgacatcgt ctacagcttg
    19621 ctgccagttt gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt
    19681 tgattctgca ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc
    19741 ttctttaggc aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga
    19801 tagcttattt gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt
    19861 ttcttttgta ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg
    19921 attggtgatg gcagattctg cataattgat ccgttgctaa tgttgccgct gattattgat
    19981 atggtatcca aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga
    20041 cttgacgaat ggcttttcca tagattcact cctaagtatt gatgatagat aaattaagat
    20101 ataattcact tacataaaaa tgacagcttt ttatttaaaa aaacataatt aaactaatat
    20161 attcacagaa atatttgtaa acaagttttc cctatataat cataccacaa actgttttct
    20221 tgactagggc aatttacgat ttaaaagtaa ataaaacatt aataagataa aaattaatcg
    20281 atttcatttt tatttccata attttcatag gtgtttttaa aagttacaca aaaaatagaa
    20341 taacaaaaaa attaacattg aattaataat agttataccc gtggtgctag ttaatttttt
    20401 cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata tagtagtctc
    20461 aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga acattataca
    20521 gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata ccacaattgt
    20581 gtaccagata agattaaaaa tcgaagaaat actgatca
//
LOCUS       DAFBQW010000043        20183 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFBQW010000043 DAFBQW010000000
VERSION     DAFBQW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.044"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="LK889_001932"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="LK889_001932"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK5096146.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="LK889_001933"
     CDS             1013..2260
                     /locus_tag="LK889_001933"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK5096147.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="LK889_001934"
     CDS             2336..2680
                     /locus_tag="LK889_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096148.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="LK889_001935"
     CDS             complement(2797..3123)
                     /locus_tag="LK889_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096149.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="LK889_001936"
     CDS             complement(3496..4698)
                     /locus_tag="LK889_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5096150.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="LK889_001937"
     CDS             4815..5330
                     /locus_tag="LK889_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5096151.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="LK889_001938"
     CDS             complement(5529..5975)
                     /locus_tag="LK889_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK5096152.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="LK889_001939"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="LK889_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK5096153.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="LK889_001940"
     CDS             complement(7854..8483)
                     /locus_tag="LK889_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5096154.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="LK889_001941"
     CDS             complement(8467..8931)
                     /locus_tag="LK889_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5096155.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="LK889_001942"
     CDS             complement(9418..10686)
                     /locus_tag="LK889_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK5096156.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="LK889_001943"
     CDS             complement(10917..11654)
                     /locus_tag="LK889_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5096157.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="LK889_001944"
     CDS             complement(11721..12290)
                     /locus_tag="LK889_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK5096158.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="LK889_001945"
     CDS             12418..13290
                     /locus_tag="LK889_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5096159.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="LK889_001946"
     CDS             13632..14504
                     /locus_tag="LK889_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5096160.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /locus_tag="LK889_001947"
     CDS             14501..16459
                     /locus_tag="LK889_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5096161.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="LK889_001948"
     CDS             complement(16710..17561)
                     /locus_tag="LK889_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5096162.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="LK889_001949"
     CDS             17684..18151
                     /locus_tag="LK889_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK5096163.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="LK889_001950"
     CDS             complement(18414..18896)
                     /locus_tag="LK889_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK5096164.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="LK889_001951"
     CDS             complement(18996..19859)
                     /locus_tag="LK889_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK5096165.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3664 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga tgaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actacttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc ttt
//
LOCUS       DAFBQW010000044        20036 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFBQW010000044 DAFBQW010000000
VERSION     DAFBQW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.045"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(56..2653)
                     /gene="adhE"
                     /locus_tag="LK889_001952"
                     /gene_synonym="adhC"
     CDS             complement(56..2653)
                     /gene="adhE"
                     /locus_tag="LK889_001952"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK5096166.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="LK889_001953"
     CDS             complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="LK889_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK5096167.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="LK889_001954"
     CDS             complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="LK889_001954"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK5096168.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4798..5496
                     /locus_tag="LK889_001955"
     CDS             4798..5496
                     /locus_tag="LK889_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK5096169.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5664..8303)
                     /locus_tag="LK889_001956"
     CDS             complement(5664..8303)
                     /locus_tag="LK889_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5096170.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8922..9953)
                     /gene="queA"
                     /locus_tag="LK889_001957"
     CDS             complement(8922..9953)
                     /gene="queA"
                     /locus_tag="LK889_001957"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK5096171.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10146..11321
                     /locus_tag="LK889_001958"
     CDS             10146..11321
                     /locus_tag="LK889_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5096172.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11325..11966
                     /locus_tag="LK889_001959"
     CDS             11325..11966
                     /locus_tag="LK889_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096173.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            11963..12880
                     /locus_tag="LK889_001960"
     CDS             11963..12880
                     /locus_tag="LK889_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5096174.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            12877..13548
                     /locus_tag="LK889_001961"
     CDS             12877..13548
                     /locus_tag="LK889_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096175.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(14089..14577)
                     /locus_tag="LK889_001962"
     CDS             complement(14089..14577)
                     /locus_tag="LK889_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBK5096176.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(14778..15044)
                     /locus_tag="LK889_001963"
     CDS             complement(14778..15044)
                     /locus_tag="LK889_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK5096177.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(15044..15307)
                     /locus_tag="LK889_001964"
     CDS             complement(15044..15307)
                     /locus_tag="LK889_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5096178.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            15783..15968
                     /locus_tag="LK889_001965"
     CDS             15783..15968
                     /locus_tag="LK889_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5096179.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(16110..17933)
                     /locus_tag="LK889_001966"
     CDS             complement(16110..17933)
                     /locus_tag="LK889_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBK5096180.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(18531..>20036)
                     /locus_tag="LK889_001967"
     CDS             complement(18531..>20036)
                     /locus_tag="LK889_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBK5096181.1"
                     /translation="FIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPL
                     LSNLQNRLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLD
                     QVIDTIGTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANT
                     MLNGNMDTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNE
                     KMPEVETAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKES
                     DFFTQPVELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNF
                     TKREQFVARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVY
                     VLAALFDNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESA
                     GGIYWPNATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         5688 a         4253 c         3765 g         6330 t
ORIGIN      
        1 ttttccaagt tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc
       61 cgttgtaagc atcaatgatg atctgtttca attcagagat caatggttct ttcggattag
      121 ctgtcgtaca ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg
      181 tttcttgtgt gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt
      241 cataaacagc cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta
      301 agaattttgc gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca
      361 tcgcatgttt ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat
      421 ttgtccgtcc atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa
      481 tacctaggaa tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt
      541 cacgtgattc catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga
      601 ttgcttgtag gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga
      661 ttgcatgtgt taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga
      721 cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt
      781 tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg
      841 atgttgtcgg gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt
      901 tacggatatc caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga
      961 acatccacat tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat
     1021 ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg
     1081 gttcaacatc tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac
     1141 gaactgtatc gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt
     1201 caacgttttc cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg
     1261 gtaatttgaa ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt
     1321 ttacagcaga gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca
     1381 atgacggaat catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca
     1441 ctaaaatacg acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg
     1501 aatggataac tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa
     1561 acgcatggtc cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg
     1621 atagcggata atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg
     1681 gtacactgat tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat
     1741 tgaccgcata ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt
     1801 ttacgatata aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat
     1861 ccacgatcac agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga
     1921 tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac
     1981 ctggacctac acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc
     2041 cacctgtcgc taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga
     2101 tcgaaggatg ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat
     2161 cacgtaccac tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa
     2221 cgattggatt tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat
     2281 tcgttgttgg agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt
     2341 tttggacatc ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat
     2401 attcggacgc atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct
     2461 ctacagccat cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca
     2521 cgatatggtc tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt
     2581 tttctgccaa atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta
     2641 ttactttagc catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg
     2701 tatatcatac aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta
     2761 tttttaaata taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat
     2821 ttattattat ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct
     2881 tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt
     2941 taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt
     3001 gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt
     3061 gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca
     3121 aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca
     3181 actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag
     3241 ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat
     3301 agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg
     3361 tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt
     3421 taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa
     3481 aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt
     3541 agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca
     3601 catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat
     3661 ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct
     3721 tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg
     3781 tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa
     3841 cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat
     3901 ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga
     3961 tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat
     4021 ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt
     4081 tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc
     4141 atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac
     4201 atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata
     4261 tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc
     4321 ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa
     4381 tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt
     4441 gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt
     4501 ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag
     4561 cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc
     4621 caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt
     4681 taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca
     4741 gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg
     4801 caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa
     4861 gctgttttac taaatctaat ccctacattg attggtattg ctactattct tcttattatg
     4921 attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg
     4981 acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc
     5041 gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat
     5101 cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg
     5161 atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt
     5221 cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc
     5281 caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg
     5341 gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta
     5401 gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa
     5461 gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag
     5521 ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc
     5581 aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag
     5641 aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac
     5701 ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga
     5761 ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc
     5821 gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg
     5881 actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct
     5941 aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata
     6001 gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca
     6061 tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt
     6121 ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca
     6181 tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac
     6241 agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata
     6301 gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca
     6361 aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc
     6421 cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca
     6481 gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct
     6541 ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat
     6601 aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct
     6661 ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca
     6721 gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg
     6781 atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct
     6841 tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca
     6901 ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat
     6961 gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt
     7021 cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt
     7081 gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata
     7141 acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat
     7201 atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc
     7261 catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata
     7321 acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc
     7381 gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt
     7441 acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc
     7501 agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg
     7561 cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga
     7621 tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga
     7681 cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct
     7741 tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat
     7801 tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg
     7861 gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg
     7921 ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt
     7981 tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc
     8041 agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc
     8101 gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc
     8161 atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc
     8221 acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct
     8281 attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt
     8341 acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt
     8401 tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat
     8461 tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca
     8521 gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca
     8581 aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt
     8641 aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat
     8701 gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac
     8761 accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg
     8821 aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt
     8881 aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca
     8941 tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc
     9001 aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa
     9061 ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc
     9121 catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga
     9181 attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc
     9241 tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta
     9301 tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca
     9361 acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc
     9421 gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga
     9481 cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa
     9541 attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact
     9601 gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt
     9661 ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc
     9721 gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata
     9781 actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc
     9841 tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt
     9901 tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca
     9961 ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca
    10021 gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt
    10081 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
    10141 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
    10201 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
    10261 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
    10321 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
    10381 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
    10441 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
    10501 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
    10561 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
    10621 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
    10681 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
    10741 cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata
    10801 caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag
    10861 atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag
    10921 caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac
    10981 gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag
    11041 aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg
    11101 ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg
    11161 ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg
    11221 catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg
    11281 ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc
    11341 atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg
    11401 ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa
    11461 aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt
    11521 tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct
    11581 ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt
    11641 ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg
    11701 ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg
    11761 tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt
    11821 ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa
    11881 ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg
    11941 cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc
    12001 tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac
    12061 catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg
    12121 aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac
    12181 tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg
    12241 aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc
    12301 taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt
    12361 tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa
    12421 agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat
    12481 caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc
    12541 gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat
    12601 tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga
    12661 tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc
    12721 tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa
    12781 aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca
    12841 acgtttctta caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat
    12901 gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt
    12961 atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg
    13021 gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat
    13081 atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg
    13141 ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat
    13201 acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg
    13261 atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg
    13321 gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc
    13381 ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg
    13441 attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta
    13501 ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa
    13561 aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt
    13621 aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct
    13681 atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc
    13741 gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc
    13801 atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc
    13861 aattcttgca tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt
    13921 cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct
    13981 tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca
    14041 gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca
    14101 tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac
    14161 caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg
    14221 tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc
    14281 cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac
    14341 gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc
    14401 tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc
    14461 tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt
    14521 cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact
    14581 tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc
    14641 cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt
    14701 ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa
    14761 ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta
    14821 ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg
    14881 ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc
    14941 aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa
    15001 tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat
    15061 caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta
    15121 aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa
    15181 tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat
    15241 ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat
    15301 tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca
    15361 aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa
    15421 agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg
    15481 gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa
    15541 gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga
    15601 ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg
    15661 attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat
    15721 tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa
    15781 aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt
    15841 atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct
    15901 gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt
    15961 acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata
    16021 gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag
    16081 agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg
    16141 acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg
    16201 agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag
    16261 ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg attttcaatc
    16321 gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct
    16381 gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca
    16441 taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg
    16501 actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga
    16561 agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt
    16621 ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg
    16681 attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt
    16741 ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg
    16801 ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg
    16861 atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct
    16921 tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca
    16981 gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa
    17041 tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt
    17101 tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag
    17161 atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat
    17221 ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct
    17281 cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc
    17341 aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca
    17401 aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga
    17461 ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt
    17521 gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac
    17581 atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca
    17641 ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat
    17701 agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg
    17761 gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat
    17821 gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca
    17881 ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa
    17941 actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat
    18001 gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa
    18061 acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt
    18121 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    18181 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    18241 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    18301 gcttgcatct gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg
    18361 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    18421 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    18481 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    18541 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    18601 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    18661 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    18721 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    18781 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    18841 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    18901 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    18961 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    19021 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    19081 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    19141 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    19201 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    19261 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    19321 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    19381 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    19441 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    19501 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    19561 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    19621 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    19681 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    19741 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    19801 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    19861 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    19921 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    19981 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaa
//
LOCUS       DAFBQW010000045        19996 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFBQW010000045 DAFBQW010000000
VERSION     DAFBQW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.046"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            4..816
                     /locus_tag="LK889_001968"
     CDS             4..816
                     /locus_tag="LK889_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBK5096182.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LK889_001969"
     CDS             813..1613
                     /locus_tag="LK889_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK5096183.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LK889_001970"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LK889_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBK5096184.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LK889_001971"
     CDS             3110..4819
                     /locus_tag="LK889_001971"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK5096185.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="LK889_001972"
     CDS             5031..9383
                     /locus_tag="LK889_001972"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBK5096186.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="LK889_001973"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="LK889_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK5096187.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="LK889_001974"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="LK889_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK5096188.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="LK889_001975"
     CDS             11221..11514
                     /locus_tag="LK889_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBK5096189.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="LK889_001976"
     CDS             11511..11822
                     /locus_tag="LK889_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBK5096190.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="LK889_001977"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="LK889_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK5096191.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="LK889_001978"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="LK889_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK5096192.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="LK889_001979"
     CDS             complement(14623..14844)
                     /locus_tag="LK889_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5096193.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="LK889_001980"
     CDS             15292..17655
                     /locus_tag="LK889_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBK5096194.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="LK889_001981"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="LK889_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK5096195.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="LK889_001982"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="LK889_001982"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBK5096196.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6863 a         3512 c         4389 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaaaacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcc
//
LOCUS       DAFBQW010000046        19834 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFBQW010000046 DAFBQW010000000
VERSION     DAFBQW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.047"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            6..305
                     /locus_tag="LK889_001983"
     CDS             6..305
                     /locus_tag="LK889_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5096197.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LK889_001984"
     CDS             378..1739
                     /locus_tag="LK889_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK5096198.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LK889_001985"
     CDS             1761..2021
                     /locus_tag="LK889_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096199.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LK889_001986"
     CDS             2035..2787
                     /locus_tag="LK889_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBK5096200.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LK889_001987"
     CDS             complement(2881..3486)
                     /locus_tag="LK889_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5096201.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LK889_001988"
     CDS             complement(3539..4513)
                     /locus_tag="LK889_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBK5096202.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LK889_001989"
     CDS             complement(4891..5610)
                     /locus_tag="LK889_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5096203.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LK889_001990"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LK889_001990"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK5096204.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LK889_001991"
     CDS             7161..8558
                     /locus_tag="LK889_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5096205.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LK889_001992"
     CDS             8639..9079
                     /locus_tag="LK889_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5096206.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LK889_001993"
     CDS             9227..9553
                     /locus_tag="LK889_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5096207.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LK889_001994"
     CDS             9554..10303
                     /locus_tag="LK889_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096208.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LK889_001995"
     CDS             complement(10383..10838)
                     /locus_tag="LK889_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK5096209.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LK889_001996"
     CDS             complement(11265..11540)
                     /locus_tag="LK889_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096210.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LK889_001997"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LK889_001997"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK5096211.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LK889_001998"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LK889_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK5096212.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LK889_001999"
     CDS             14770..17436
                     /locus_tag="LK889_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5096213.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LK889_002000"
     CDS             17800..18552
                     /locus_tag="LK889_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5096214.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LK889_002001"
     CDS             complement(18574..19728)
                     /locus_tag="LK889_002001"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBK5096215.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6337 a         3473 c         4060 g         5964 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcaggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taat
//
LOCUS       DAFBQW010000047        19338 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFBQW010000047 DAFBQW010000000
VERSION     DAFBQW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.048"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            148..585
                     /locus_tag="LK889_002002"
     CDS             148..585
                     /locus_tag="LK889_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096216.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            680..1564
                     /locus_tag="LK889_002003"
     CDS             680..1564
                     /locus_tag="LK889_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK5096217.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1788..4955
                     /locus_tag="LK889_002004"
     CDS             1788..4955
                     /locus_tag="LK889_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096218.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4975..5520
                     /locus_tag="LK889_002005"
     CDS             4975..5520
                     /locus_tag="LK889_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBK5096219.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5548..6003
                     /locus_tag="LK889_002006"
     CDS             5548..6003
                     /locus_tag="LK889_002006"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBK5096220.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            6000..6758
                     /locus_tag="LK889_002007"
     CDS             6000..6758
                     /locus_tag="LK889_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK5096221.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6844..7584)
                     /locus_tag="LK889_002008"
     CDS             complement(6844..7584)
                     /locus_tag="LK889_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5096222.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7645..8898
                     /locus_tag="LK889_002009"
     CDS             7645..8898
                     /locus_tag="LK889_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5096223.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNTFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9220..9447
                     /locus_tag="LK889_002010"
     CDS             9220..9447
                     /locus_tag="LK889_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK5096224.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9570..9896
                     /locus_tag="LK889_002011"
     CDS             9570..9896
                     /locus_tag="LK889_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK5096225.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9964..10677
                     /locus_tag="LK889_002012"
     CDS             9964..10677
                     /locus_tag="LK889_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK5096226.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10987..11838
                     /locus_tag="LK889_002013"
     CDS             10987..11838
                     /locus_tag="LK889_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5096227.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11959..13824
                     /locus_tag="LK889_002014"
     CDS             11959..13824
                     /locus_tag="LK889_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5096228.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13872..15281
                     /locus_tag="LK889_002015"
     CDS             13872..15281
                     /locus_tag="LK889_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBK5096229.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15389..16090
                     /locus_tag="LK889_002016"
     CDS             15389..16090
                     /locus_tag="LK889_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5096230.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16249..16728
                     /locus_tag="LK889_002017"
                     /pseudo
     CDS             16249..16728
                     /locus_tag="LK889_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            17576..18151
                     /locus_tag="LK889_002018"
     CDS             17576..18151
                     /locus_tag="LK889_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBK5096231.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18151..18591
                     /locus_tag="LK889_002019"
     CDS             18151..18591
                     /locus_tag="LK889_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK5096232.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18607..19308
                     /locus_tag="LK889_002020"
     CDS             18607..19308
                     /locus_tag="LK889_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5096233.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         6486 a         3217 c         4138 g         5497 t
ORIGIN      
        1 taaatcaaca gtagtttaat tactacttga tttagttctt tttattttaa aaattttttt
       61 tacttagaat ccagcgaccc tagatatttt tctgtataat cttaaataaa gaaaacaata
      121 tcaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct tttatttcta
      181 gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg aagtacaatg
      241 gatcatttgt atataaaata tattcatcaa ttttccgttt tggtgttagg cacactaatt
      301 atagcgggga ttgtcgcatt tattattcaa ggccgtgaca tattaacagt acatcaagag
      361 tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa caaaaagtgg
      421 aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc taagattttt
      481 actttattag gtatacaatg cctatcattt tcttttattt cctatatcat tgttttaatt
      541 ttcctcagtt tcatagtatg gatgttttat cttgtcaaaa aataaatgaa tgtttcatga
      601 acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta aagaaatcaa
      661 tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg
      721 tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg tgtttgatga
      781 agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc
      841 tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca acgaaggaat
      901 cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat tgggtacggt
      961 gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga tcaatcattt
     1021 ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact attttatgaa
     1081 tgtcgtcgca attggctcta tccctgaatc aatcaatgat gtagatgcgg agaagaagac
     1141 gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt ccacacaatc
     1201 ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca gtaccttgct
     1261 gataggacta actaactcag tcggcggttt tgaaacatta cttccaaatg caaaagtgga
     1321 tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata cgcttaaaac
     1381 attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct accttacttg
     1441 tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg ttgatggaga
     1501 tgaaggggat aaattacctg tgacaatcaa agtattgccc tcacatttac aggtttattg
     1561 ttaaaatgga ggttgtgacc gaggagttgg cgggagaaca ccgcttgttc gggataaggg
     1621 actgtgacaa gacttttgtc acagcctttt ttttgtacaa aaaagaaatt tacacctaat
     1681 agaaaaattt ttccataaaa atacatattt taaatatgaa agtacaattg gaaatagatt
     1741 aaaacgataa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatgtg
     1801 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
     1861 ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
     1921 ctactttttc agaagaatcg attacttctg aaactacaga atctcaaagt gaggcttcta
     1981 ctaataaaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
     2041 ctgatagcag tgaagcggca ctggcaagag ctgtttcagc ggatgggtac agcgaagctg
     2101 ccacaacaga agaactggcg cagcttctcg cagacgaatc agtaactaag atccgactga
     2161 ttcagccttt gactttagac agggaattag agatcaaacg agatatcgtt attgattttg
     2221 gtggattcgc tcacaatttc ggcacacacc atatttatat caatgagata cctcagtcga
     2281 tcgagttcca gaattttaaa ggaacagctg cacatccagg tggattgaca ccaaatatcg
     2341 atggaaatgc cattatcatt gcttatatga ccgatttttg gggaagcaga tatcatttta
     2401 cgggagaaat aaaatttacc gggacacttg atttgtatga tgggtccaaa ttaggactga
     2461 tttacgctcc tcgtgcaact gtcacattag atggtgtttc cggtgtgttg gatgtccaac
     2521 cagtgaagga aggactgaat gccgctccgg gtaaagccta tttttgtaga agctatcaat
     2581 tgaatgtgat caatggtagt caactttatg gaccgtatct agggaaattc tacggttttc
     2641 tagattcagg agatgagact ggtagtggaa aaacagcgcc aggaatccat atcttaagcg
     2701 gttcaaaggt ctctcttgat tatgatcggg cagatggcgt tagcgatgaa ggagaggcag
     2761 tcgatacgct acctggtaat gtcatgtttg aagtcagtgg ttcgggaagc gaattttcgg
     2821 taaatacaaa gatcaatatt accaatgaca acaatcgcgg aatcatccaa atgagaggtt
     2881 caggatcgaa agtcactgta tcgaatggtg ggaaaatcac catcaataca gcaactacgg
     2941 gcggatttcg cttgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
     3001 cggtcaaaat ggctggagat gacgatcaac ccgggaacaa tgggatgcgt tttgtaggaa
     3061 aaggcctttc tttgatcgta gaaggagccg gatcagttat cgacatcgat aagcagagtg
     3121 ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa gctgacagta accaatggag
     3181 gctcattgag ggtaagaaat acaggtgatg gcaaatctta cgtgaacaga ctcaatcaag
     3241 gaaatcaagc gatccaattt tatgacgctt ctggttttct agaaagtcca ggttcagctg
     3301 attttacagt cactggagaa aaatcaaata tcgatatacg cgcagattcc ggagcaactg
     3361 tagatacaac tggagatatt gatttaaatt ttacggcagg tgaaaaaact tatttagtca
     3421 tggtcgggaa aacaggggaa gataagaccg gcatcatcag tggggatgtg ttgaatgtgg
     3481 acttagaatc tcctacctat tttgatttcc aaaacaaacg gattggaagc gagaccaatg
     3541 gcataggcgg ctgggtattc caaggaaatg gagattcttc tttaaagatc gcccattcag
     3601 aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa ctttgaaaaa gacccggatt
     3661 atttttctga tatgacagac ttatcgatca atgggaccga cttaggcaat ttcgtatcaa
     3721 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
     3781 atcgaatcag tgccaataat cagctcccga tgattgaatc cttaagagtg ccaacgaatg
     3841 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
     3901 atgcttggac aggcgaagta gaagtgactt tacgtattac ttacgcagat acgacgaaat
     3961 catctgtaga aattactgct ctcacgcaag gacaaacaga tgaaaatgaa ggggcaggat
     4021 ataacccatg gggagaaggc gaacaaggcg gcttgttcga agttcaagta cccaatgatg
     4081 agagattaaa aacggaggat caagtgcaag ttgttgctgc taaaaaagta agaggtggcc
     4141 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgaacctg
     4201 ctaatttaag taccacattg atcaatgcag cttctcgcac gatcacaggt acaggtgaag
     4261 caggagcctt ggtttcattg aaaaaaaatc aagctgtttt gtcagaaaca actattgatg
     4321 atacagggga atttgttctt tctatcccag aaaatcaact ttttgcagga gatgtattag
     4381 aaattgtatt aaacgaccaa gcaggagaag ctactgctaa gggtgtgcag aacaagccaa
     4441 gtaccaacga tgaaattggg aatcacaatc caagtgatac gccagttgtt tatcatgatg
     4501 ccccgccttt tgcacctgct acgaagatcg tagtagaagg tggtctctca tttcttgccc
     4561 cttccaaact cgattttggt cagatcaaag caactggttt gacacaagaa gcatttggcc
     4621 aaatggatat acaggaaaaa ttatggattt atgatcagcg agaaacgaaa aatccgtgga
     4681 tcttaacgat aaaattatct cagcctttca caaatgatgc aggattttct atgcaggatg
     4741 tcctatattt caagaaagga gaggagtatc aattagtgaa tgaagaagaa ctggaaattg
     4801 tcagaggaca aaattcaacg aatgtgcaag cagattattc agaatattta aataacggtc
     4861 gggcgtttaa acttgtctta accaaagaaa atcagatagc tggtaattac tcagcagaga
     4921 taacatggac attagcagat gcgccgacag aataaagatc aaggaggatg attgatgaat
     4981 cgaatatgga ataaaagtca tcgaaacaac tgctctttac ttatgatcct tctccttgga
     5041 ttcatccttt ggcagacacc tgtccatgcc tccgaattca attttgcggt agattttgaa
     5101 ttaccggcta atcagattga taagaacaaa acatatataa atgtaaggat ggagcctggg
     5161 aaaacacagg aaatccccta cacggtcttt aaccgaacag ataaatcagt aacagttgag
     5221 acattgatcc actccgctac gacgaattca aacggtgtga tcgaatatgg agaatcaaaa
     5281 agtaaatttg atgaaacatt aagttatgat atgaaaaact tagcaaaaac agtgggagaa
     5341 ataactgttc ctcctcaaag tgcggtcact gagacacttt cactgaaagc gccagaaaaa
     5401 gagctggctg gtgtggtcgc aggagggatc acgtttcaag aaaaaaaaga gaacgtttct
     5461 aacaaaagta cagttggtgt cgtcaatcag ttttctcaag tgaaagcaat cttgatttga
     5521 aatagcgaaa aagagtcttc cccagatttg aaattaaaaa aagcttatgc ggatcaaagc
     5581 gatggaagaa atgtgattaa ggcgaatctt caaaatccaa atgcttctta tatgttcggc
     5641 ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag agccttacct ttcaataaaa
     5701 aaagaaaaaa tagatgtagc acccaataca aattttgatt tacttgtccc attagaagga
     5761 aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg gtgcttggaa agaaaaacgc
     5821 tggaatctcc ataccgattt tgaaataact gatgatgcag cggaccaatt gaatgaaaag
     5881 gatattgatt taaaaaatca gccagaaaca tttgactggc gatggctgat acttgttgga
     5941 gtcattctta ttggcttatt tgctggatta tttattttaa aagaaagaag gaaaaagaaa
     6001 tgaaaaaaac aattgcatta agtaccgttc tagctggaag tctattatta tgtacacaaa
     6061 gtgtttttgc tgagaataag agctcagcca ctacagaagg aacgatttct gtgaccaaac
     6121 cagatgctgc aaatcctgaa gacgttgttc cagttcctcc tgttgatcca aatgatcctg
     6181 atactccttt tacacctgac gaaccaaatc agggaacatc cggattactg acgattgacc
     6241 aagctccaga tcttgatttt ggaacaatca agttaggtac agcaaaaaca gtatatgctg
     6301 cattacaaag cggaacggac agtactggag cacaaacaca agtacagaat ttcgtacaag
     6361 taacggataa aagcggaaat tatgccggtt ggaaattatc agtcaaacga acagaattta
     6421 ttggtacaac atctgatacg aataaactaa ctggttcaaa ggtgaaattc aaaaacgccg
     6481 tgattcgaaa tagcgcaact aatagcagtg gaatgccatc taccgtttct ggaactggcg
     6541 ctgaaggaat cgaaatccca gcagatcagg cagtagatct agtaactgca ggagcaaatg
     6601 aagggattgg cacatggatt tattcattag gagagaatgt ggaacaaggg agacaatcag
     6661 tggaattatt tgttcctgta tccaattatg ctgtagacaa ctacgtttca acattcacat
     6721 ggtcgttaac agatgctcct acagaagagg aagcgtaaaa tcatatcaac gatttcaggg
     6781 agtggagccg attcaaggcc cactccctga aatctgctat gcttacgtct cagctcttat
     6841 tcatcaactc gaagcagttt tttggaagtc atggaaacgt tcctttgtct tttcaaagat
     6901 ctttcgacca acgagtaagg agtcaccgtt gaaaaattct aacacataat gagcagaatc
     6961 aattttctgc aattgcgata agttgataca gtaagatttt aaaagtaaaa agtaagcata
     7021 cttttctaac tctgtcttca attgttttaa agaagtattc actaatttat cttctgtagt
     7081 tgttttgacc ataacttggt ttttcaaacc tttgatactt tcaatatata aaatttcccg
     7141 aggatacagg acgagacttc cacctttttg atcaataaag attttatcga catttacgat
     7201 ctgctgttct ttccaattga tctgttctag cacttgacga atttgttcag ataaattcct
     7261 gtcttttctg atatatccaa tcggaaggat tttccgattg atgatctcta ctgcaaaatt
     7321 cgtataagcg gtgaaaaaaa caatattact atgtgcattt tttttacgta tttgttcggc
     7381 gacatcaata cctgtgagtg attgcttcaa gtcgatatcg ataaagaaaa catcgtgatt
     7441 atcaatagtg cccaactgga tcaactgatt gatttgagag atagaagtga gctgcacgat
     7501 attctcgata tgatgattga ccaattctct gatgatccat tcgctgtgaa actggttatc
     7561 ttctatgata aaatactttt tcatatgcag gctcctatta aaaaaatatg tgtacatttt
     7621 gaaacaaaac aatggaggaa taagatggtc gcaacagtga cactttattt gacctttact
     7681 ttgattttag atttaggtat ctatttattc ttttcgaaac aactgctagt aaaaaacagc
     7741 tttggttgta tagctttgat ggtggtgaca cttttacttc ctatccctaa agtgagcttt
     7801 ccttacctag ttggcttatt attgatgttt cttttttatt ttactacgaa aaacctgttt
     7861 atcagctgtt tgatcagtaa tctttttttt gctatcactg gactcagctt ttttctggtt
     7921 tttgatctga tggattattt aggaaagaga catcttggaa cagcaagttt ttttgcacta
     7981 cttatcgcat ttattttcta tctattagta aaaagtatag acaaacagac gagatttctc
     8041 aacttgcttt ttacttatcc aactgaacac tattttggag gtatttgtat tttattaagc
     8101 tggggcgtga tttacttggt attgggaagt gttcacttcc gctcatctaa ttatctgctt
     8161 ctatctcttt tgtcgatagt catcagtttt ttttctttgt ttttttccat tatgttgatc
     8221 agtttccaag taaaagaaag acaacatcag gaatcgcttc aactgtatca aataaatgaa
     8281 gaatattacc atcatctaga ggaattcaaa catgattata aaagtctact gttttcatta
     8341 aaaattactc ttcaggaaaa taaaaaagaa acgttggaat ttttagaaat attagaagat
     8401 cattctgcag ctatttttga aaaaccgcaa atacaacagc taacaaatat tgtatcccca
     8461 gctgtcaggg gtgtcttttt aaaatttcta gaaaaagcag aatcggagaa aatcccgatc
     8521 caagtagtta ttcctgtcga agtgaaaaca atcccgattg atctggttgt ttttattcgc
     8581 tgtttatcta tttttatttc taacacattc gatcatcgtg tagccgatca gagtccgatt
     8641 acgatcaatc tgctggaaga aaaagaggga tcacgtttta gtatagcgaa caaggcaaat
     8701 ccaacgaatt tgacgctatc ggagatggtc aaaagaggaa aaacctctaa aaaaaatggc
     8761 gggttaggtc tatacagtgc gaaaaaaatg ttggatgttt acgaaaatgc agaactaaaa
     8821 atcgaatttt caaaacagaa tcactatttt tttgtagaaa tgtatctggc atctagaaag
     8881 agcaaatcag cctactgact tactgtcaaa ggataagttt gaggaaagtc tacacggaga
     8941 tagagacaac aacacagcat attcaaaaga aacagaagcg tgatgaccgt tgtcaccctt
     9001 ctgtttcttt tgctatgtag ttaaacaaag tagttgacac ttccgctact tagtattagt
     9061 acgaaagtat acttcatgat actaagtaga taagaagtga aaagcggaac ccacgatcgt
     9121 caaaatgcta actgctttgc ttacaccaga taaagggaag agcaatatcg cttattttag
     9181 gcgtggagaa gcaagcaaat ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga
     9241 gagttagaac aagatgaaag tatccatcga aaagcctata accaagtccc gcctaaagta
     9301 gagtactcgc tcaccgaaga aggaaaatca ttaagagaga ttctagtagc gttgtctgtt
     9361 tgggaagaag aacgtatcga aaaacaaaaa gcctcaaggg aagaagacac gctactgcat
     9421 acacatggtg gctacctaaa ttattgattt tctatgacat ttattttaaa taaaagaaaa
     9481 caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa
     9541 gtaagaagtg attggaagaa ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta
     9601 gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatc tctatttctt
     9661 tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa
     9721 cacaattaga acgaatcaaa gaagaactgg cagatgtatt gatctattca tacatgatgg
     9781 cagacaacct tcacctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt
     9841 tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat
     9901 gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag
     9961 aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg
    10021 ttattagtag gactagcttt gattttcaat gaacagatca aggattattt tgtcagagag
    10081 acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat
    10141 aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg
    10201 tcgcaaatga gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca
    10261 atcaatttac cggttttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca
    10321 ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg
    10381 aaccctgaat tgctgtttac accgctagaa aatttagaga tgggtgccaa gatctatttg
    10441 acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca
    10501 gatacccaat tactgaatga agtcgaggga aaagaaattg tcacactaat tacttgtggc
    10561 gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacatcgata
    10621 aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat
    10681 actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa
    10741 accaatttgc tgaaaaaaag agaaacgcaa agaggtgcat ctctgatttg gttgttttct
    10801 agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa
    10861 ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca
    10921 gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga
    10981 agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc
    11041 ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaagat tggggacgag
    11101 atttcgccag aagcggtcca aaaagaattt gttttaagag attcttttgc taaaaaccaa
    11161 ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc
    11221 gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc
    11281 gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta
    11341 ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg
    11401 ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt
    11461 cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca
    11521 atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa
    11581 tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa
    11641 ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca
    11701 aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa
    11761 atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa
    11821 aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat
    11881 aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa
    11941 agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta
    12001 ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta
    12061 aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc
    12121 gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa
    12181 tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat
    12241 attttgaatc gtctgatcga tattatctct agtatattta caccttttct aggggcgatg
    12301 gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca
    12361 gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta
    12421 cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta
    12481 gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct
    12541 atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata
    12601 ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt
    12661 ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta
    12721 acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc
    12781 aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa
    12841 gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa
    12901 caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg
    12961 gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct
    13021 tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct
    13081 ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga
    13141 ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc
    13201 attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt
    13261 gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac
    13321 aatcaattag aaaacaaaca tgcgaatgca ggagaaccga ttacttcagc acggcatacc
    13381 ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt
    13441 tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca
    13501 cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg
    13561 actgacggag cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat
    13621 ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt
    13681 tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg
    13741 aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa
    13801 gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa
    13861 atttttatgg ggtggagcaa ctgctgccaa atcaattaga aggggcttat gatcgagacg
    13921 gaaaaggact atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg
    13981 gcagtgaaga atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag
    14041 gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca
    14101 aatgttatcg tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc
    14161 ctaacgaagc gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata
    14221 ttgaacctgt gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg
    14281 gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag
    14341 agagatacag cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc
    14401 actttccagc tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata
    14461 ttttccaagc atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg
    14521 aattgcgttc ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg
    14581 atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct
    14641 gcacggatgt ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat
    14701 atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat
    14761 atccagtgga ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc
    14821 cacaagcagc aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg
    14881 caagtgaatg gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat
    14941 acaatcgtta ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata
    15001 tggaagagga tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg
    15061 aagcgatggc taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat
    15121 gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg
    15181 tggatctaga cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt
    15241 ggtataaaga agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa
    15301 aagaacacac ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt
    15361 tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt
    15421 cgcagataaa attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttgcc
    15481 gacagaaagt gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat
    15541 cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct
    15601 aagcaatcgt ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa
    15661 ctcccgatat gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga
    15721 agatcctttg ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt
    15781 gaacaatcag ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag
    15841 cagagaagaa tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat
    15901 tgactcattt tctgatgatt tctcggcagt atttttaaca gaagaagaac aaggaattct
    15961 gaaaacagac gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga
    16021 aatgattgaa tattcggagg cggtctacca tacggagctg catccttacc atattgatta
    16081 tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    16141 gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc gcagatacac
    16201 ggctgtttct ggattaccat ttattctgga taagggggtg tggcacgatt ttcagaagga
    16261 atctatgaac caatcactgt attgatttta gcttcgatgg taaccccatt cggcttgacg
    16321 atcgcttatt tcttagggaa aatcattcgt aaaaatatac tcaatcgtca agaaatcgat
    16381 acattgaaga ccgcttttcc tatggggatc tgtcagatca cagagggatg tttccctatc
    16441 gtattgaacg acttggcacg caatgtcata gcaacgggag tcggtgggac agtaggtggc
    16501 ggattaagca tgttttgggg cgcagatagc cgtattccgg catcgggaat gtttgcagtc
    16561 gctacgatga ccagaccatg ggcattcatt ggtgcattgt tagctggttc ttttgtcaca
    16621 ggaataacca ttttattatt gaaaaaacca gtagatccga atgcagaaat catccaagaa
    16681 gaaaaagagg aagaagacat atcttgggat gatttgacaa ttagttagga gaggcagaat
    16741 ggattacaag aaaaaagtac aatagatgta ccttgagaca ggaatggtat gaaagaatca
    16801 gagttagaaa gaatcgaata tacagatttt ggtttaggag cgatcgaaac tgtccagagc
    16861 atcggcaccc tccttgaaat gacgatgaag gaaaaatgag aacctttcgt tgtcgaaagg
    16921 gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta ctattccaaa
    16981 aaggaaagaa agttattata cagtaaaaaa agaaattatt cttcatgcta gagatcagta
    17041 cacgttcgag cccaatatca aacactagtt ccaggcaggc gcaacaggtg cggttatcac
    17101 tgaattttct tccagcagtg acgattcaag tgatattttt acaaatccag ctatttatcg
    17161 ttaacaaaaa gatttttagg agaagctgat gaacaaaaat gatcaaattt tgatcgttta
    17221 gttcgtcggc ttttttattt agagaaacat catttatcag tgaaacataa cacgagaaca
    17281 aaatgatttt ttcgttaata aaagaaacaa gtatttgttg tttaaaataa tacacgttat
    17341 tggcatatag gacgaaatcc cagtatcttc tataagtaag tcatttcata tcgatgaaaa
    17401 cacgttattt atttttgttt tatctataaa atggctgaaa aatgattttt ttctaaatac
    17461 tatatatagt ggcgtctatc ctatatacca ctaaaaaact acaaaatcta gtcttttatt
    17521 ttaaaaaaac acgtgtagaa tgaagataga cttttgagtt agggagtgtt tttttatgaa
    17581 acttactgtt ttttgcggtt cccgttttgg taataaagaa agttacaaat ttattgcgca
    17641 aacgttagga aaatacatgg cacaagaaga aatcgaactg gtttacggtg gttcatactc
    17701 cgggattatg ggagtgatta gtaaaacagt actggaaaat aacggaaaag tcacaggtat
    17761 ctatccgaat gggctttttg aaactgaatt gcctggaaaa gatgttacta catttattcc
    17821 tacagaaacg atcgatgaaa gaaaggtatt attatttgaa aagggagatg ctgtcctcgt
    17881 tttccctgga ggtctaggta cattggagga attctcacaa cttctttctt ggatagcaat
    17941 cggtctgacg cccgacaagc caatcggaat attgaatatt ggcggttatt acagcggctt
    18001 gcaacaatta ttagaaactt ttgcaaaaga ggattttatg gataaaaaat ggttggatca
    18061 agtaatcttt tccaacaatc cattggaact ggtcaatgaa ttacgagcag tatctattaa
    18121 aaatcagtta ttattgaagg aggcaaaata atgagtcaaa tatatttagc aggaccattt
    18181 ttttcagagg agcaaatcga tcgagtaagt cgtatcgaaa aagcgttgga ggaaaacaaa
    18241 acagtcacaa gtttctactc tccacgtcat caccaagaaa gcaattatga actattcagc
    18301 gctggatggg cgcaggaagt atacgaaaaa gatatggaag aactgacaaa tgcagaattt
    18361 gttgttgcta tccttgactt tgagcatcaa acaatcgatc caggaacggc ttacgaatta
    18421 ggtgtggcaa ctatgttgaa aaaaccgatg atcattgtac aagaagagac ggtacctacg
    18481 aacttgatga ttacacaaag tctccatacg tatttaaaat cagaccaagc tgtacgtgaa
    18541 tatgattttg aaacattgcc tgttgaaact tacgtaggcg agtacttata gaagaggaag
    18601 ggttgtatgt cagtgatttt gatttcagga acgattggtg caggtaagag cagtttgacg
    18661 gatatgcttg caaaagagat tgattctaaa ccattttatg aaaatgtcga agataacgaa
    18721 gtattgccgc tcttttattc aaatcctgag cagtacgctt ttttattgca aatcttcttt
    18781 ttgaacaagc gatttttagc aatgaaaaat gctttagtga acgatgacaa tgtgctagac
    18841 cgatccatct atgaagatag cctgttgttc catctaaacg ctgatttagg tcgggtaacg
    18901 gatatcgaag tacagcagta cgatagctta ttagatacga tgctaaacga gttagatgat
    18961 gttgctccaa aaaaacggcc tgatttgatg gtccatatca aagtttcttt ggacacgatg
    19021 ctagaacgta tcaaaaaacg tgggcgtgac tatgaacagc tagaaagcga tgagacgtta
    19081 tatacctatt atgaaacgtt gaacacccgc tataatcaat ggtatgaaga ttttgatgta
    19141 tgtcctaaaa tacaagtgga tggggataaa ttcgattttg tagaaaatcc agaagatgca
    19201 aaaaaggtga tccgtcagat ccaagaaaaa ttagctgaat tggaaggaaa gcctgctgga
    19261 aaatatttta cggataacca gcatgcaaaa ggtcaagaat taggctaaaa ataaaaaacc
    19321 aaacgacggg aaagaaaa
//
LOCUS       DAFBQW010000048        19190 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFBQW010000048 DAFBQW010000000
VERSION     DAFBQW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.049"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="LK889_002021"
     CDS             complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="LK889_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK5096234.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1819..3042)
                     /locus_tag="LK889_002022"
     CDS             complement(1819..3042)
                     /locus_tag="LK889_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBK5096235.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3073..3912)
                     /locus_tag="LK889_002023"
     CDS             complement(3073..3912)
                     /locus_tag="LK889_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK5096236.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3929..4609)
                     /locus_tag="LK889_002024"
     CDS             complement(3929..4609)
                     /locus_tag="LK889_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096237.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4611..5684)
                     /locus_tag="LK889_002025"
     CDS             complement(4611..5684)
                     /locus_tag="LK889_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5096238.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(5740..5978)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(6021..6254)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6331..7002)
                     /locus_tag="LK889_002026"
     CDS             complement(6331..7002)
                     /locus_tag="LK889_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5096239.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="LK889_002027"
     CDS             complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="LK889_002027"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK5096240.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10426..10863
                     /gene="ndk"
                     /locus_tag="LK889_002028"
     CDS             10426..10863
                     /gene="ndk"
                     /locus_tag="LK889_002028"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK5096241.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10885..11751)
                     /locus_tag="LK889_002029"
     CDS             complement(10885..11751)
                     /locus_tag="LK889_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5096242.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRAL
                     EKKLLAIAEPSN"
     gene            complement(11911..14004)
                     /locus_tag="LK889_002030"
     CDS             complement(11911..14004)
                     /locus_tag="LK889_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5096243.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(14753..15376)
                     /locus_tag="LK889_002031"
     CDS             complement(14753..15376)
                     /locus_tag="LK889_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096244.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(15535..16927)
                     /locus_tag="LK889_002032"
                     /pseudo
     CDS             complement(15535..16927)
                     /locus_tag="LK889_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(16959..17930)
                     /locus_tag="LK889_002033"
     CDS             complement(16959..17930)
                     /locus_tag="LK889_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5096245.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            18304..18729
                     /locus_tag="LK889_002034"
     CDS             18304..18729
                     /locus_tag="LK889_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096246.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         5547 a         4066 c         3232 g         6345 t
ORIGIN      
        1 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
       61 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
      121 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
      181 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
      241 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
      301 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
      361 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
      421 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
      481 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
      541 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
      601 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
      661 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
      721 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
      781 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
      841 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
      901 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
      961 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
     1021 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
     1081 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
     1141 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
     1201 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
     1261 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
     1321 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
     1381 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
     1441 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
     1501 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
     1561 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
     1621 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
     1681 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
     1741 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
     1801 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
     1861 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
     1921 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
     1981 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
     2041 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
     2101 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
     2161 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
     2221 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
     2281 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
     2341 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
     2401 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
     2461 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
     2521 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
     2581 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
     2641 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
     2701 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
     2761 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
     2821 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
     2881 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
     2941 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
     3001 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
     3061 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
     3121 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
     3181 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
     3241 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
     3301 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
     3361 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
     3421 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
     3481 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
     3541 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
     3601 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
     3661 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
     3721 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
     3781 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
     3841 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
     3901 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
     3961 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
     4021 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
     4081 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
     4141 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
     4201 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
     4261 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
     4321 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
     4381 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
     4441 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
     4501 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
     4561 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
     4621 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
     4681 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
     4741 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
     4801 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
     4861 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
     4921 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
     4981 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
     5041 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
     5101 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
     5161 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
     5221 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
     5281 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
     5341 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
     5401 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
     5461 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
     5521 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
     5581 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
     5641 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
     5701 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
     5761 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
     5821 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
     5881 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
     5941 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
     6001 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
     6061 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
     6121 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
     6181 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
     6241 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
     6301 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
     6361 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
     6421 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
     6481 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
     6541 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
     6601 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
     6661 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
     6721 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
     6781 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
     6841 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
     6901 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
     6961 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
     7021 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
     7081 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
     7141 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
     7201 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
     7261 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
     7321 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
     7381 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
     7441 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
     7501 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
     7561 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
     7621 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
     7681 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
     7741 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
     7801 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
     7861 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
     7921 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
     7981 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
     8041 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
     8101 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
     8161 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
     8221 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
     8281 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
     8341 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
     8401 ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca
     8461 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
     8521 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
     8581 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
     8641 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
     8701 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
     8761 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
     8821 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
     8881 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
     8941 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
     9001 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
     9061 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
     9121 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
     9181 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
     9241 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
     9301 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
     9361 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
     9421 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
     9481 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
     9541 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
     9601 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
     9661 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
     9721 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
     9781 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
     9841 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
     9901 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
     9961 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    10021 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    10081 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    10141 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    10201 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    10261 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    10321 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    10381 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    10441 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    10501 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    10561 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    10621 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    10681 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    10741 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    10801 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    10861 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    10921 ctctaaggcc ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    10981 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    11041 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    11101 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    11161 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    11221 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    11281 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    11341 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    11401 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    11461 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    11521 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    11581 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    11641 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    11701 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    11761 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    11821 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt
    11881 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    11941 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    12001 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    12061 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    12121 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    12181 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    12241 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    12301 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    12361 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    12421 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    12481 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    12541 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    12601 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    12661 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    12721 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    12781 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    12841 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    12901 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    12961 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    13021 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    13081 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    13141 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    13201 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    13261 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    13321 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    13381 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    13441 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    13501 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    13561 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    13621 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    13681 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    13741 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    13801 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    13861 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    13921 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    13981 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    14041 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    14101 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    14161 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    14221 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    14281 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    14341 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    14401 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    14461 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    14521 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    14581 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt
    14641 caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
    14701 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc
    14761 cataggattg atattgtcct tgtgatgact cattcgtaat aattgaggct tgattttctt
    14821 cacttcccgt tatcgtttgt ccactgccag aatctgaagg acttgttcct gtcgactcgt
    14881 ttttttgaga agagacagtt gttttcttga cttgttcatt tgtctttttc cattccgcta
    14941 aacgatcggc gaccactttt ttgtctactt tttcagaata agcggtgatc acttttccat
    15001 ttttcaaacg ggctactttg tcgccatcat gagtcaaaag caaaacggtc aaatgcttgt
    15061 ctttaaattg atattgtctg agagaaatcg cactaagtac gtcatcgata ttaataaatt
    15121 gattttcttc ttttcctttt ggcaaataaa tgtccaattc gcctttttcg taattggcag
    15181 ttattttata ctccgcccac caataagttg agaattcttt tttgaaatta tctactgatt
    15241 ctttgcttgc tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg
    15301 aaaatgctgc ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg
    15361 gtcttttttt cctcatgctt tcctccactc ctatttaatt ttgactattc tattatatac
    15421 aaaaaatgaa atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt
    15481 tcacaaaatg gtaagaatgg agaatttcac gcatttgctc tcaactattc tttcttaagc
    15541 ctcaaatccg tttttatctg atacgtcttt gaaccaatga cccgattttt tgattgttct
    15601 ttttccatct tgtgctaagt ctactgaaat aaagccatat cgattcttat atgcattggt
    15661 ccacgaccag ttatccatac atgttatgat agcctaaaca attggcacct tcttgaatgg
    15721 cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca tcgtggatca
    15781 tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt tcagaaataa
    15841 agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag attccttgtt
    15901 catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga
    15961 aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt gcttttaccc
    16021 ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg ataaccgtca
    16081 aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca atcaattcac
    16141 ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta
    16201 tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt ggtgtcagat
    16261 tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg atggctaacg
    16321 ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct gactcttctg
    16381 gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca ttatgagtaa
    16441 accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat ttttcatagg
    16501 cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga
    16561 aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca aaataacgat
    16621 gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc
    16681 attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt
    16741 gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg
    16801 gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct gaaatccttc
    16861 cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga aattgatatt
    16921 ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc atgtatgata
    16981 ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg ttttaacagc
    17041 tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat tttcggaagg
    17101 atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt
    17161 ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta attgctgaca
    17221 aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta
    17281 gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac cgcttctttg
    17341 gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat aaatccgatt
    17401 tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc tcgatcattc
    17461 actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat aactggaata
    17521 ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa gacaatcagc
    17581 ccatcgatca catgcttcat caggtattca attttttgtt caattttttc tggctgatcg
    17641 gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc gatccctgag
    17701 acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat agagaagctt
    17761 cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct
    17821 tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta acgggaaact
    17881 gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat acattctcac
    17941 tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac cgcattcttt
    18001 gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga gcaacttcct
    18061 ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat atagcatttt
    18121 tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt
    18181 ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca
    18241 tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa tgggggaagt
    18301 attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc
    18361 tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta
    18421 aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg taaagatatg
    18481 gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat
    18541 cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc
    18601 aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaaaca
    18661 acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg caacatctac
    18721 agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta
    18781 aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg attattgtac
    18841 atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct tgagcatatg
    18901 cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt atcctgttca
    18961 aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact atggctgatt
    19021 tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa aacaaacagt
    19081 ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat atttgaatat
    19141 aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt
//
LOCUS       DAFBQW010000049        19183 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFBQW010000049 DAFBQW010000000
VERSION     DAFBQW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.050"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(35..1186)
                     /locus_tag="LK889_002035"
     CDS             complement(35..1186)
                     /locus_tag="LK889_002035"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK5096247.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1199..1636)
                     /locus_tag="LK889_002036"
     CDS             complement(1199..1636)
                     /locus_tag="LK889_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5096248.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1651..3417)
                     /locus_tag="LK889_002037"
     CDS             complement(1651..3417)
                     /locus_tag="LK889_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK5096249.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3681..4358
                     /locus_tag="LK889_002038"
     CDS             3681..4358
                     /locus_tag="LK889_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096250.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4361..5488
                     /locus_tag="LK889_002039"
     CDS             4361..5488
                     /locus_tag="LK889_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096251.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5688..6128)
                     /locus_tag="LK889_002040"
     CDS             complement(5688..6128)
                     /locus_tag="LK889_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5096252.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6139..8172)
                     /locus_tag="LK889_002041"
     CDS             complement(6139..8172)
                     /locus_tag="LK889_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5096253.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGLFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8174..9877)
                     /locus_tag="LK889_002042"
     CDS             complement(8174..9877)
                     /locus_tag="LK889_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5096254.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9971..10921)
                     /gene="manA"
                     /locus_tag="LK889_002043"
     CDS             complement(9971..10921)
                     /gene="manA"
                     /locus_tag="LK889_002043"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBK5096255.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10960..11985)
                     /locus_tag="LK889_002044"
     CDS             complement(10960..11985)
                     /locus_tag="LK889_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK5096256.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12194..13063)
                     /locus_tag="LK889_002045"
     CDS             complement(12194..13063)
                     /locus_tag="LK889_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBK5096257.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13522..15414)
                     /locus_tag="LK889_002046"
     CDS             complement(13522..15414)
                     /locus_tag="LK889_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096258.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15411..17138)
                     /locus_tag="LK889_002047"
     CDS             complement(15411..17138)
                     /locus_tag="LK889_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096259.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17154..17804)
                     /locus_tag="LK889_002048"
     CDS             complement(17154..17804)
                     /locus_tag="LK889_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK5096260.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18008..18304
                     /locus_tag="LK889_002049"
     CDS             18008..18304
                     /locus_tag="LK889_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK5096261.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18496..19116
                     /locus_tag="LK889_002050"
     CDS             18496..19116
                     /locus_tag="LK889_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK5096262.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
BASE COUNT         5453 a         3954 c         3381 g         6395 t
ORIGIN      
        1 aatttcgtca cattcccctt tttcgtatcc tattttaagc gacttgcttg gcataacggt
       61 tcacgttttg cttgatattc ccgattgttt tcggatcatt gattcctgtg acttcttgaa
      121 tcaattgttc caagttttca tggatcttca tttcatgcaa ttgacggctt tgttcatctt
      181 caggatcata gtaattaaaa ataatcccca ttgttgccgt caaatgtggg caagtcaagt
      241 tatgttcttg tagttctctc actggacgtg taaatctttc ttgataacct aacttacgta
      301 atggtgtccg agcgacacgt gtgatcgcat cagagatatt tttgttttgg aagcgatgaa
      361 tgattttttc aatatacgca tgatgctgct ctgcatcaaa cccccatttg gcaatcagca
      421 aacttcctgt ttcctgcaaa actgatttta attgtgccac aactaacgca tcttgcattg
      481 cttcatcaat ggtctggtaa cctaacaagg cacctgtata agcgacagtc gcatgaccag
      541 tattgacact aaatagtttg cgttcgatat atggttctag atcatctaca tataaaaccc
      601 cttttaattg gattttttta ttttttcttt ggctatcgtc aatgacccat tctttgaaag
      661 gttccacttg gacgaataaa ggatcttcat ggtgttgtaa tgggacgatt cgatcgactg
      721 ctgcatttgg aaagccgata tatttgtcag taaatgatgt ttccggaaga tatttataca
      781 cttctttttc tagaaatgct gaaccgccaa tcatattttc acaggcaatg atatctaaag
      841 atgttgtgct tccttccttt tctctttctt ggataccttt tgcaatcaac tcagcaataa
      901 aaggtaagat attcgggcca atggcggtag taactaaatc tgcttcagcg atttcttgga
      961 ttactttttc tggatttgtt tgattgttga ttcctttgac attttttaca gtgatttttt
     1021 cttttgattc atctgctaat tcgatttcgt attcttttct ttcatttaaa gcatcaatga
     1081 tcgtgtgatt cacatctacg aaagtaatct caaatccatt agctgccaaa acctctccaa
     1141 taaatcctcg accgatattt cctgcaccaa aatgtactgc tctcattttt ctctctcctt
     1201 aagcaattgc tagattttcc gttacttctt ctttgctgag cgcgtctgct agctggacaa
     1261 cattgtccat atcactgcaa taaagtgcga tttgctgtac aagttgcaaa tgctcatctc
     1321 cgactcctgc aataccgaac aaaacagtcg caatcttttc ttcttcttcg gtaccaaaat
     1381 ctacaccatc tggtacttga acgacacaga tgccggattt tttcacatat ttttttgctt
     1441 catctgttcc atgaggaatc gcgataaaat tccccatata aacagataac attttgtctc
     1501 tatctaccat ggcatcgata tattcctcat caacgcaccc tgcttctaca agcatctgac
     1561 cgcaatagcg gatcgcttct tctttggttt taaaagatcg attgagctcg atcatgtcga
     1621 atggcaaact ttgcatgctg ttttcctccc ttaagctgtc tgtttcaaat attgtgcaat
     1681 cgattcgtat ttttgcggat tgaccatgct gttgactgat aaatgaatgg attgaggtgc
     1741 agccatctgt actttttccg ctaaactttc tttggctacg atcagtgaat caggttcatc
     1801 ttttaaatct tggatattga cttttcttaa cgggatagtt aaatggttct tgtctaatat
     1861 atctttgagc acggtcatcc ccattgttgc tgatccggaa cgatcatcgt ggacaagtag
     1921 aatctctttg atttgtttga tgtttgctga aagaaactct tctgctggct cattctcaat
     1981 ttcagaaact gatgttttct gcttgctgct taattcttga atgatttcat catatcgagg
     2041 tgaattcaaa aagttttcta ctgagacaaa acgcgcgctc ggtgcttttt gttttgctct
     2101 ttcttgtagt tcgacctgag taacgatcaa tgtattcgag tcatctgtca gttggctgat
     2161 tgcttgattg gtcaccgttt gaggcaagtc tgcttcctgt actttttttc tcaagatcga
     2221 agcgcccatc gcacttgatc ccattcctgc atcacaagca aaaatgattt tttgtacgcc
     2281 agagaatgta gctccttgag gaataccttg ggcacctttg gaagcttgct tagctgcctt
     2341 tgtctcttga acagattttt cgaagtcttc atttgtatct ttcgcatccg ctttcaaaat
     2401 tactgcagaa ataccaaacg aaacagctgc ggcaacaatc acgcccaaaa taacaggaag
     2461 gtaagaattg aacggtgtca tcgctagaat cgcaatgatc gaccctggag aagctggcgc
     2521 tcttagtcca gcatccatca attggaaaat gaaacttcct gacattcctc cgcctataac
     2581 tgataagaat aataacggtt tcatcataac gtaaggaaaa tagatttcat ggatcccgcc
     2641 taaaaagtga atgatcatgg ctcctggtgc tgaagatttt gctgctcctt tgccaaacaa
     2701 agtaaaagca agtaatacgc caagtcctgg accaggattt gcttcaagca agaatagaat
     2761 agattttcca gcctctgcta cttgttcagt tcccaatggt gtcaaaatcc catgattgat
     2821 tgcattattc aaaaataaga tttttgctgg ttcaataaaa atacttgtca atggaatcaa
     2881 atgggcagat aaaatgatct ctacaccttt tgccattgct tgagtcaatg catcgacaac
     2941 tggaccaatc gcagaaaaac ctaataacgc taatccaaaa ccgataagac ctgctgaaaa
     3001 attattgact agcatttcaa aaccggattt gatccgtttt tggaatagct gatcaaattt
     3061 cttgatggca tatccaccaa gaggtcccat gatcatcgcg cctagcatca ttggaatatc
     3121 tgttccgaca atcacaccca tcgttgcaat tgccccaaca actgatccgc ggtcaccagc
     3181 aattactttt ccgccagtat aaccgatcaa taaaggaatc agataagtta gcattgggct
     3241 taccatggta gccaaagcct catttggtaa ataaccatct gggataaata gagccgttaa
     3301 tacaccccag gcaatcagtg ctccaatatt tggcatgacc atgcttgata aagtactgcc
     3361 taatttttgt accctcgctt ttagcgaaat gttgtttttg ggaatcgtcc gttccatttt
     3421 gtctctccct ctctcctttt gatactctaa ttgtatccgc ttactaaaaa gagaaatagt
     3481 cgtactttgg cacgaaactt tgtgccaaag gtttgagtat gtcagcaaaa taaaaagatt
     3541 tcggtatcag aaaacttcaa ttttaaataa aactgttatt ttaatttttt tataatatgc
     3601 aaacaataac ttttgttagc atttaattta gatgttttcc attctattat gttattaaat
     3661 tttatattgg aggcctatct atgacattac tgatcaaaaa catgaacatg aaacgtggta
     3721 aaaaacgtgt gattcgacaa ttgaatcttt tagtagaatc aggagaagta gctgctttga
     3781 ttgccccgaa cggatttggc aaaacaactt tgctgaatgg cattgcccaa ctgctgccaa
     3841 caacttatga tcaattgtcc cttcacaaca tctctcctac tgcttataca gattatcgcc
     3901 ggtctttctt ttttctagaa tcaacgaatc agttatccga ctatctatcg gctgttgatt
     3961 atttgacttt gatcaaatac tattggcaga gcaaagtaga atgccaaact gttttccaac
     4021 gattgaagat gactgagtac cttactttgc cgattggaaa aatgtctcta ggaatgaaac
     4081 aacatgtctt gcttgctgct tacttgatca gtgattcatc tgtcatgtta ttcgatgaac
     4141 cgttgaacgg attagaccca ggaagtattg atatcttgaa tagcctcatc agacaatggc
     4201 agaaagaggg gaagacgatt cttctttctt cccacgctat cgcgaatatc caatcgattt
     4261 gttctaaagc atgttttcta aaaaatggag aaatcgtcac agaatcctca aatatggaag
     4321 agattttagc tatttatcgt aaattatatg aggtgtaact atgtctttac ttacatttga
     4381 acgcaatctc ttactaaaaa acaaagtaaa ctttttattt cctctattgt tggtcgtgct
     4441 ctttgcattc ccactttttt ttgatcataa acttgcttat acagaattcg atgaactgaa
     4501 tcataactat gaagagatgc aacgtttgat tgaaacgttg aaagaagatg agaatgagaa
     4561 agaatttgtg gaaagtttag agaaaagcaa caaactgatc gaagcaatct tacatgcaaa
     4621 aaacacaggg aatgtccagc aaatggtcga agcaacttat cattatgaaa aagatatact
     4681 ggatcgactg atttctggtc aaagacaagg aattccaatc attgaacaac aaaaacgtgt
     4741 cgagttgctc cgttatatga aagaacatca gatccaaaga tattctattt ttgacttgcc
     4801 agcgcatctc cctttagcaa attactacga aaatattttt tcaggaatga tttctagttt
     4861 tttgatttta tgtataaccg ccttgtttct ttcttccatc atctcttatg aaaaaagaaa
     4921 gcaggtaatc tctctagtca atctattacc ggatagtatg gtaaagaaac atagcatccg
     4981 ttttaccatt tattatggtg cagctatgct aagtctcgtg atgccatttt tgatcgtctc
     5041 tatactagta ataatcaaaa atggtttggg agacttccgt tatcctgtag gaacgatcat
     5101 tggtcaggag atacgaatcc ttccgatgta tgagtatctt ttccagagct ttctgttcct
     5161 gttgttgtgg gtgctctttt taagtacaat cagttttctt ctatctgctt tatttgaaca
     5221 ttctctagtc aatcttttag gtacacttct ttgtcttttc ttagcagagt atcgcttgtt
     5281 ttcttcgatt ggatggatag aatcaatcag tcattacttg ccaacttctt atgttgattt
     5341 tcaaaatgtg atcataggag gagatatttt ctcgccacta gcttcagaac aggttacttt
     5401 catgaatggt atcttgacac ttggtatatg gagtatcgtt ttattattca taggtatggg
     5461 cactatctat ataaaaaaga gctattaaaa cacaaaaata ataggggcgg tgacaaaaac
     5521 cttgtcaccg tcccctattt caaataaacg gttttctaga ataaatagtt ttcaaaagct
     5581 tgcatccgca gtaataagaa cctcccaaca aaaatagctc ctcttatttt tatcttggtt
     5641 gaagttatta cttgatgcaa ggcagctttt tttactgtga acattggtta tatatgtcca
     5701 tgttcaaaaa tcatttggac ttcttcaatg gttttcgcat cactgagcgc cattacttgg
     5761 tcgatgtctg aacagaaaaa ggctaaatct tggagcaccg ctaactgatg ttcttttttt
     5821 aaagccacta caaataagag tgtcgcgatt tttggttcct cttttgttcc aaagtccaca
     5881 ccatcaggca cttggactaa atagattcct tcttctacta cttcttcatc tttgtcagtc
     5941 ccatgtggca aggctacgaa attcccgata tagacactcg ctttttcttg tctttcgatc
     6001 attgattgga tatatgccgg tttgattccc ccttgttggg tcatccactg gccgatctcg
     6061 gtcaacgctt ctttccaatt ttgataggat tgatcgagca atattttttc tgctggtagc
     6121 tgtatcatct tttcaccttc atttctttat ttcttcaatc aggattttcg ctaacaactg
     6181 ataaacgatt tctttatttc ctgagcggaa gatctcggtg ttcaagtcat tcatgatgat
     6241 tgctccactg atctttccta aaagaatccc gtctttttct tggattggcg aaggcgccag
     6301 cataactaac atacgggaca aatcgactgg ctgtctatcc atcccttcaa tggtcaacgg
     6361 atgtttcaaa ttgaaaatac aaaagatcgg tttagaaatc aatgagctgg atgtatggaa
     6421 taaccccata tttgtatgag gaataccgat aggtgcttgt tcatatcgct taagcagccg
     6481 gcgatgaacc acctcttcat ccgttactag tccttcagga aaataaggaa ataagctgcg
     6541 aatcgtttcg gaaatcgtct caggattatc cagttcttgg ataaaaaaaa gaccgagtaa
     6601 ttgattgatt tgatccatga aatgcattgt ttcctcataa gtatcttcta tcatccactc
     6661 gttattgctg ttatacgtaa ttttttgcct ttttttcttc tcaatatgcg aaaaagcttc
     6721 ttttagctgc ttgacttcat cttctagcag tagcggggag actaaaatat attttccacc
     6781 gtatcctggt aaaatagatg tagaaataac aacatcatat tcttcttgta aatcgatttt
     6841 ttttaaatca gcaatccgat agaaatgaat ttgttggatg aagggaaaat accttttcaa
     6901 tcgtagttcc aacatgctag tagaagctaa tccacttggc gaaaatcctg caatactgat
     6961 attgctttct ttcggacttc gttccaatga attcgcaaaa tgaaggacca tataagcgat
     7021 ctcttcttcg gataattttt tttcttgaaa aatccgctct actgctttac ggattgcatt
     7081 cgcaacttcc tcgtattgaa ccatgatttt ttctaaaatc ggattggtca gtgtctcttc
     7141 ttgtaaaacg gctcttgaaa acacaccaga taaatgagtc aacagcattt tgtacaaagt
     7201 ttcatcttca taaaaatgga cattcgtctc ttcacttact tcttgaatca gttgttttac
     7261 taaataagca agctgtgtat cgaacgtctc ttcaaagaaa ttctgatcaa atgagttcgt
     7321 gaagtcattt aataatcctg caaaaaagac gatctcattt actggataaa gttgttttgt
     7381 ctcagaagcg atccgtgaaa acaatcgttt agctgtctgt agtaattctt tgtttgccag
     7441 actattatat gtctcatttg tcagtaaatg atttttagtc acacgctcca tcgtcaatgt
     7501 cagcatcaaa accaaatgtt caagctttct atcacttaga ttggtctttt tttggatcaa
     7561 ttcttccccg attactttgc gtgcaagaga tagatgttgc ggatcaacga atcgaaaaaa
     7621 ataattcagt gatgcatcga aagaaatact atgaaaaagt tcatattcat tgatttctaa
     7681 ttctaataca ttcgctgtca gaagccggcg gtatttttcc ggtccttcga tctcgtatcc
     7741 ctggttccga atgatttcca aaggcagttg gcgaatactt tcttccaact gtttgatatc
     7801 tgcataaaaa gtggtattac tgatgcgata tgattccatg aaatgagcaa gactcaacgg
     7861 ctctgtcgtc agtaacaact cggctaaaat cgcgtgttgt cgtttagcag tggataagtc
     7921 attacttttc ccatctgtat tttcttttaa ttgctctaat acttcttctg atgcctcaat
     7981 gacaaatgag cctctagctg cttttttcaa agaagcattt accgatttca atgagacttc
     8041 aagattatct aattcgcggt aaatagtccg tttactgacc ttcaataaat tcatcatttt
     8101 ttggatagat acaggtgacg ggctactgag aaattctgct aataattgtt tttctctggg
     8161 cgataaatac atcttatatt tctcccttca cgagtttttc gtattcattc gtagctaccc
     8221 aatgttcaac tagaactagc cgagggatct tttcttctag ctgttcagtt acagctagtt
     8281 ctctttgtgc gatataaatc gtttcttctt ggattttgat tttttctatt gattcttttg
     8341 agataacaaa ctgttttttc gctttttttg ctaattgact taaaatcgtc atgcccatag
     8401 tttgtgagcc gcgtctgttg tttttataca aaaatacaat attagcaggc gacttttttt
     8461 gttctgtgat atctgctatc tctttttttt cttcccgctt tggaaattgg atcgcttctt
     8521 ttattcgtga cagcaagata tctgtttcca aaaagtgatc cagtacatag atttgacttt
     8581 gaggtacttt ttctcttgca agacccgcta gttcatgttg cacgataacc agctgattta
     8641 cttggttatt caattcatag atagagctgt atccgacaga aagatcgatt ttttctgctg
     8701 ctaatttctt tttcagcagg gcggctccca tagcacttga tcccatacct gagtcgcaag
     8761 caaaaatgac ggaatgaatg atagatggtt ccaagatttc tcccctttct ttgaccgttt
     8821 tgttctcttt ttctttattt ggtaatttca atagtatcgc tgcacaaatg aaggtcacaa
     8881 atgtactgat tgctatccct atacttaccc cagctagttg tggataaggc gcattggcaa
     8941 aaatcacgag tagtgaccca ggcgatacag gtgcagctaa tcccgtatct aaccattgaa
     9001 atatcaacgt gccgcttgcc ccaccagcca ttacagctaa aaatagatac ggtcgcagca
     9061 agacaaatgg aaaatagatt tcgtgaatcc cgccaatcgc atggatcatc aatgctccac
     9121 tcgcatttac tttttcctgt ttttctgcaa aacaaaggat agaaagtaag atccctaaac
     9181 caggtccagg atttgcctcc atcaaaaata aggaagagta cccgactgtt tgtgagaatt
     9241 cgattcctaa aggtgtcaat acaccatgat tgattgcatt attgaaaaat agtactttta
     9301 gaggttctaa gatcagactg acaagtggta acagattcat tgtgactagc caagacacaa
     9361 cggaggcgag caacaagtga acactattta aaactggttc aataaaaacg attccaatta
     9421 tacaaataac gctaccagtc agtcctacta gtaggttgct gacaagcatt tcatatccag
     9481 ccttgaattt cacgtggaat ctagaaacaa attgttcata tacgatgccg gcaagcggcc
     9541 cgataatcat cgctcctacg atttgtggag cattggaacg agcgatcaac cctaaagaag
     9601 caattgcacc ggcgactgcc cctttttgtc cccctaccga tttcccgcct gaataactga
     9661 tcaagatggg taaaagcagc tggatcatcc aatggcatac ctcttctaat gtggtgtgca
     9721 aatcgccaga cacacctttt gttgctaatg acaagattcc ccaagcgata aacagactta
     9781 gatttggcat gatcatttca tttaagtgat gtccagcttt tctcagttgt ttcatagaca
     9841 cgcgactgct gttgtttgct ttccatttgt attccatctt tttcaccata cttctattta
     9901 ttgatggacg gattttatgt agaaaaagca gcaaacgaaa aaccgtctgc tgctttcagc
     9961 tatctgggaa ctatttgcct ggttcagtcg caatgattgc tagttcacct tggaccgccc
    10021 actcagaaac gccatttgga ataatacagc ttgttccttt ttctaatgga tatgtttttc
    10081 cgtcaacgac aaggtctcct gctccttcga ttaccgtcat caatgtataa ggggcttgtt
    10141 ttttgaaaga tgtgattcct ttaatatccc actcatatac attgaaaaac tcagtttcta
    10201 aataagtgac gatcgatgaa ttcccttttc ggacttcttt gatttgtaat tctggtgttt
    10261 tcgcagggac agttgttaca tcgatcgatt gttggatatg cagctctctt gtttgtcctt
    10321 gcgcatcttt tctgtcgtag tcatacacgc ggtaagtcgt gtcagaactt tgctgtgttt
    10381 ctaaaatcat gattccttta ccaatagcat ggatcgttcc gcttggtaca taaaagaaat
    10441 cacctttttt aactggtact ttcttcaaaa ggtcatccca gcgcccatct ttgatcatct
    10501 cagctagttc ttctcttgtc tttgcatgat gaccatagat gatttctgca ccaggttcag
    10561 catcgatgat ataccagcat tcagtttttc ctaattcacc ttcatgtttc atcccatagg
    10621 catcatctgg atgtacttgt acagatagat catcttctgc gtctaagatc ttgatcaata
    10681 atggaaacac tggttctgaa ggatggccaa acaattcctt gtgttctgac catagttcat
    10741 ctaatctttt ccctttgaat tcgccatttt ctactacact gacaccatga ggatgcgcac
    10801 tgatcgccca atcttctccg attttatcgt tagggatatc aaaaccaaag actgaacgta
    10861 aacgacttcc tccccaaatt ttttcttgaa aaactggttt taaaaacata ggttcctgca
    10921 taattcaaac tcctctcatt tttcatagct attatagcat tatttcggta ataccgcttt
    10981 ctctttcaaa aagttttcaa caatttcatc tcgacatatg agatactccg cacgatgatc
    11041 gacaggatgt acgcgatttg acaaaaatat caacgcttct tgtgttttta tatcaatcaa
    11101 gaaaaacgta cctgtataac cagtatgaaa aagaatcgca tgaccgtctt ctacatcctt
    11161 ttttaagtcc catcctaaag aacgatagcc attttttttc ggtgtttgat cttgcagtaa
    11221 agaatcaatc gtctcttctt ctaaaaaacg accattacgg atttttccct tgttcaaata
    11281 catttggaca aaagtaatgc tgtctttcaa atcaataaag agacccgcgt tacctgcatg
    11341 ttcagccaaa actcttgctt tcggatcatg agtttgtcca cgcaaaacct cacccgaagg
    11401 cagttgttct gtgggaacga tttttttgga aggaaaagga agaaataagc tgttcatcat
    11461 tcctaatggt tccaatactt gttcttgaaa gatttccact gcagtttctt ggtaaatttc
    11521 ctctagcatc aacccaagaa gaatcgtacc ggcatctgta tattttactt cttttcctag
    11581 ttgctcacct ggttctagct tcaaataagc ggcgatcaat tcttcctgag atagcttatc
    11641 tctgtttggt atccatgttt tgatatcaga agtatgtgtc agtaaatgac gcaaagtgat
    11701 tgtctgattt tcaaattcag gaagatattt atgaagcggt tgatcgagtt ctatctttcc
    11761 atcttctaat agacgtaaaa ctaatgaagt ggttgcaacg actttcgtca gtgaggctgc
    11821 atcaaacaac agatcttctg tcatcagctg cttttcagga acgacttggg caaagccggc
    11881 tgtaaatgtt tctacttgat tttcttttat gaaggcgact gctacacctg gataaaatcc
    11941 ttcttccatc tttttcaaaa taagctgttt cgtgtgtgga tacatctttt tctcctgctt
    12001 ttctatcaga ttcttacagt ccaagcttaa agcttaatct atgaggaatc aacctttttt
    12061 acacaaaaaa actgcgcgaa aaaccgcgca gtttcccccg gtatactatt ttctctttct
    12121 atctctagaa taagcgatag tatgaccgtt cataaaatga cgagacatgc gacaatcgtt
    12181 aagaaagaaa aagttaagcg tcgcatcgaa tgtcagtttt attcttttct tttggctcac
    12241 tgacgaacca ccattcgatt cctatagcat catagattgt gagtactgtg tgctttttgt
    12301 ctaagaagaa atcaactgat agttgctgca agtgagcaat cagtccttcc atgctttcag
    12361 tatcgatcat gaatttcaga aaatcaagtc caatcacttc atctgtaggc aaatcaattg
    12421 cgcctccgct agcttctgtc aaaccaacat ggaaatctcc ttcattcaat acatggatgc
    12481 cctcttcttc attatgtacc ttcaaaccaa gtacatgatt aagaaattgg cgttcttttg
    12541 ttaagttagt aacatttaaa tggatttttt caaaataaga acctgttgat agtttactga
    12601 atttttcttc agattttctt aataatgctt cttgatccat tgtttctgga atttctcttt
    12661 ggtcgttctt attctcttca tggtaaattt ccaattcatt ctcttctggg tctacgagca
    12721 aaatccccat acgtccatga tcttctaaag cttctttgat tggataattc attttacgga
    12781 tacgacaaag gatatctgcc atttcttctg tgctagggat aatcaatgaa aaacggttca
    12841 gctttttgat cacccccatg tgatcattcg ctctaggact ttcttccagc agcaacattt
    12901 cacttccagc ttcttctgtt cctaaaatgg ctaattcgtt ttcttctctt ttcaaatcaa
    12961 agccaacaac gttcttgtaa aaatcaatca ttttatctcg gtctttcacc cgaatggcta
    13021 cactttttaa ataggtggca gcccccaatt gaaaatcttt catatttctt gctcccctct
    13081 acgtcaagct ttattttaac gatggattct tcttctttca agcaataact ttgattttgt
    13141 taaaaataat gtgagaaaat tcgcattttg cttgtttttt gtaaattaat gagtaaaaac
    13201 tcttataaaa agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca
    13261 aaaatagctc ctcctatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga
    13321 acaccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagatacaaa
    13381 acggaaaaat agcttctcat attttgccgt tttttgagct aataccgaag gacagctgat
    13441 tgaacaccgt ttattcggaa aaagggactg tgacacgact tttgtcacag cccctaaaaa
    13501 aaataataaa tttgtactat tctatgaagc ttcttcttca gaaaactgac tgttataaag
    13561 atctgcatag aatccgtctt tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga
    13621 accatgattc atcacaataa tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac
    13681 aacgaaactt gtccggtttt ccaataggcg gttcatcgct ttttgaatca gtatttctgt
    13741 tcgtgtatct acactagatg ttgcttcatc aaggatcagg acatctggat ttgctaagaa
    13801 tgcccgcgca attgtgatta gctgacgctg cccttgcgaa atattgcttg catcctcgtt
    13861 taatacagtg ttatatcctt ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt
    13921 agcagcctca atgatttgtt cgtctgttgc atcgtcgttc ccatatttga tattgtcata
    13981 gatacttcct gtaaacagcc aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc
    14041 atcacgtgaa agatcacggg tatctacacc atcgtaacga atactacctc cagaaacatc
    14101 gtagaaccgt tcaagaaggt tgatcagtgt cgtcttccct gcaccagtcg gacctacaat
    14161 agcgaccatc tcaccaggtt tgacatccaa gttaaaatca gtcattagca agttatctgg
    14221 tgtgtagccg aattgaacat tttcaaaacg aactttgtat ggtgtatctt tttcttctgg
    14281 aaggccagat ttttcattgg acatctcttc ttcatccaac acttcaaata cccgttcggc
    14341 tgaagcaact gttgattgga tagtgttcat caaacttgca agttgagtga ttggttgaga
    14401 gaactgtgtt gtatattgca agaacgcttg aacatcccct aatgtcatgt tcccatttgc
    14461 aacttttact ccgcctagta cagcgacgaa cacatagcct agatttttaa cgaaattcat
    14521 caacggcatg atgattgccg aaataaattg agctttccaa ccagcggcat acagattctg
    14581 attttctttt tcaaatactt cctggtcttt ttcagtatga ttgaatgttt taacaatggt
    14641 atgaccacca tatgtttctt ctacttggtt attcaataaa cctaaacttt tttgctgatc
    14701 tgcgaaaaac acttgtgatt tcggcgctac gatcatcacg atgatcaagc ttaaagggat
    14761 catcaataac gcgactaacg tcaattgcca gctgattgaa agcatcatca cgatgacccc
    14821 aatcaatgtg actgcactag ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat
    14881 gttatccata tcattgattg ctcgagacat gatatcccca tttgtatgtg tatcataata
    14941 ggaaatcggt acacgattca ttttggcttc caaatcatga cgtaattcat aaactgttcg
    15001 ttgggaaaca cgtgtcataa taaattgctg taaaaagttg aatacagcag aaatcaaata
    15061 catcacgatt actgtcgcaa tgatctgcgc gattttatca aagtctatcg gaaattttgt
    15121 tacttgttgg ccttgttgca tctgttgagc gccagccatt acacctttga atatttctgt
    15181 ggttgctttc cccaagacct ttggtgtttg gatttggaag atcacggctg ctattgctag
    15241 tacaaataca gcgatgatcg caaccatacg tttagacata tagccaaaca atcggcgtac
    15301 cgttccccag aaatttttag gcttttcacc ttttacaccg agatttctgc caggtccacc
    15361 atgtcctgga ccgcctgctg gtccatgaga gggacgtgtg tttgttttac tcatgctaag
    15421 tcctcctctc ttagttgtga atgaacgatt tcttgataag tttcgtttgt tgctaatagt
    15481 tcttcatgtg ttcctttgcc aaccagtttt ccttcatcta gcacaaggat catatctgca
    15541 tccattactg tacttactcg ttgagcaact aaaacggtga tactttcttt catattcgtt
    15601 ttcaatgctt ggcgaagatt ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca
    15661 aacacataga tatctgctgt tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt
    15721 ccaccagaga agtttcctcc tccttgttcc acatggctat ctaagccatc ttccatctct
    15781 gaaacaaaat ctttagcttg tgcgatttct aatgctttcc aaatcatttc atctgttgca
    15841 tccggcttac catactgcat attgtctcgg atcgttccag agaacagaac tgctttttga
    15901 ggaacaaagc ccattaattc tcgcaaatta tattgagtca tgtcactaat gtccgttcca
    15961 tttatttgga tcgaaccaga ttcgatgtca tataatcgag ggatcaaatt gactaaggtc
    16021 gttttacctg aaccagtccc ccctatgatt gcgacgatct cgcccgactt ggcttggaaa
    16081 tcaatatctt ccaatgcaag tttttctgct ccatgatatc gataattgac atgattgaag
    16141 gccaatgttg cgttttttcc agcaaaagaa actgttttag gattttcagg gtcatggata
    16201 ccgatttttt catccaacac ttcattaata cggtcagctg aagcttgtgc acgaggcacc
    16261 atgataaaaa tagcggaaag catcataaag ctgatcaaga tctgcatcgc ataagtcata
    16321 aatgcaatca gattaccgac ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa
    16381 gtgattgcta cgtttgtacc gctcatgatc aatgtcatca ttggcatcat caatgcgacg
    16441 atcgtattaa ctttgattgc cgtatttgta taatctttat tcgctaaatc aaaacgattt
    16501 tcttcaaagc gtgtcttatc gaatgctcga atcactctga cccctgtcaa tccttctcga
    16561 aataccaggt ttaaacggtc agttttcttt tgcatacttt tgaataatgg aacagcaaag
    16621 tacatgattc cgccgatcaa aataatcatt actgggatga caactaagaa gatttttgtc
    16681 aactgatgat ctttttgata agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg
    16741 atcatcatac gtaaaaacat ttgtgtaacc atttggattt gagtcacatc attggtcgtt
    16801 cttgtgatca aagaggccgt tccatattta tcaaacgcat cttttgtcag actctctgat
    16861 tttttataga catccgtacg caattgctta cccagtttct gtgattcacg agtagcaaaa
    16921 aatgtatttg cgatagcagc taagatactg atcaaagaaa agccgatcat cacaaaacct
    16981 gtatgccaaa tataatcgac atctccttgc gctacccctt tatcaataat attggaggta
    17041 agtgtcggca aatataaatc tgccagtact tggatcacca tgaaaactgc agctgcaatt
    17101 gctgaagtca aggagatccg ttttacgatt tttagcatct agttcccttc tttctattta
    17161 tatactccgt ttttcagcca agaaagtttc aaattgaatt cactgattgc ttgctctact
    17221 gagtaatctt cagatgttga aaataagcga tacgaatgcg caattgttgt aaatacagta
    17281 ttcatcacta attggatcag taattccaat tcatgatcat tttttacttt cagcttcgtc
    17341 cgatcgatca attcaagcaa ctcttttgca tcttgtttgt gttctttatg tggatcgtga
    17401 ggcgtttttt ggcgactcgt atgaggagcg acttttcttg aggcacggta atccatgttc
    17461 ataaacaagt tacgataaaa agaagcattc tcgccttcca aaatctcata aatcatttta
    17521 gaaaaatagg attcgaatcc tttgaataga tcaccatcgg attctttcat tagttgtaac
    17581 atatctttag agctgtcacg tctcattgtc tcaaagtaat aaaaataaag atcctcttta
    17641 tcttcaaaat actgatagaa actgcctcta gggatatccg ctaatttgac gatattagca
    17701 atcgaagctt cttttaaggg tactctagaa aactcgattc tcgcagcctc gattagtctt
    17761 ttttgttttt ctttaggcaa atggaaaaat gtctgtgtcg gcatatggat tctcctttct
    17821 ttcaagatga cacattgtca tatgacgcta tgtcattata acacctccta cctgttttgc
    17881 aagacatttg catagttttt ttcattatca tccgatacat taaaaaagtg ataagaggaa
    17941 tccttccatt tgaattaatg agagttgccc attaaaatag ccacatacta aacaaaggat
    18001 gagataaatg gcccaatcaa ttagtgtcca agacttcaat aaattgcctc tcgatgctga
    18061 cagcatcgtt ttagatacaa gaaatacctt agattacaca aaaaaccact taagccactc
    18121 gctttctatc cccgctgcta tcttgccaag acaattaaaa aatctagatc caaagaaact
    18181 ttattatatt ctaagctata caggcaggcg atcagaagca ttagccgatc aacttactgc
    18241 taatggtttt cgtgctgttt atatcattgg tgggatggaa gctcttcagt cgagtgttgc
    18301 ttgaatattg cttgttagat tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt
    18361 ttataccctg tcttttttta gttatttatt cttcaacctt atcaatatga cttgatagcc
    18421 ttttcttttt cgagtaaact gattttgtta gtcatagtta taacaatcat agcaataaaa
    18481 ggagaaggta agcatatggc atctgtttta gtagtcaaag gtcatcccct aactgcagaa
    18541 gaatcgcgca cagttaaggc attgacgtct tttttaacaa gttataaaga aaatcatcca
    18601 gatgatgaag tgactgtttt ggaactttat cgcgatgata ttccggaaat cgatgaagaa
    18661 ttattatcag gttgggaagc attacgtgct ggtgcagaat tcacttcctt atcagaaagt
    18721 caacaacaaa aaattgctcg tttcaatgaa ttgactgatc agttccttgc tgctgataaa
    18781 atcgttatcg caaacgcctt atggaatcta aatatcccaa caaaattaaa agcttggttc
    18841 gataccgtca atgtagctgg aaaaacgttc cgctatacag aaaacgggcc agaaggatta
    18901 gtgacaggta aaaaagcact gcacatccaa tctaatggcg gtgtatacaa cggacaagac
    18961 tttgcctctc aatatgtaaa aggtatttta aactttgtag gcatcgatca agtagatcag
    19021 ctattcatcg aaggaatcga ttatgaccct gaccgtgcag atgagttgat gcaaaatgca
    19081 ttagataaag ctgctgcttt aggtaaatca ttttaaaaat tgagataaat gcatacaaaa
    19141 gagacagagg aaaaaatctt caaagcagca tttgtgcctt gga
//
LOCUS       DAFBQW010000050        18895 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFBQW010000050 DAFBQW010000000
VERSION     DAFBQW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.051"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(21..251)
                     /locus_tag="LK889_002051"
     CDS             complement(21..251)
                     /locus_tag="LK889_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096263.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="LK889_002052"
     CDS             complement(687..1253)
                     /locus_tag="LK889_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5096264.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="LK889_002053"
     CDS             complement(1253..2209)
                     /locus_tag="LK889_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK5096265.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="LK889_002054"
     CDS             complement(2209..2865)
                     /locus_tag="LK889_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK5096266.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="LK889_002055"
     CDS             3235..5253
                     /locus_tag="LK889_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5096267.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="LK889_002056"
     CDS             5680..8094
                     /locus_tag="LK889_002056"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK5096268.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="LK889_002057"
     CDS             8442..9995
                     /locus_tag="LK889_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK5096269.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="LK889_002058"
     CDS             complement(10376..12160)
                     /locus_tag="LK889_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096270.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="LK889_002059"
     CDS             complement(12175..13896)
                     /locus_tag="LK889_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096271.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="LK889_002060"
     CDS             13996..14757
                     /locus_tag="LK889_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5096272.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="LK889_002061"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="LK889_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="LK889_002062"
     CDS             16664..>18895
                     /locus_tag="LK889_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096273.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DAFBQW010000051        18703 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFBQW010000051 DAFBQW010000000
VERSION     DAFBQW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.052"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(41..1021)
                     /locus_tag="LK889_002063"
     CDS             complement(41..1021)
                     /locus_tag="LK889_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5096274.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1045..2529)
                     /gene="galT"
                     /locus_tag="LK889_002064"
     CDS             complement(1045..2529)
                     /gene="galT"
                     /locus_tag="LK889_002064"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBK5096275.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2541..3530)
                     /gene="galE"
                     /locus_tag="LK889_002065"
     CDS             complement(2541..3530)
                     /gene="galE"
                     /locus_tag="LK889_002065"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK5096276.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3778..3945
                     /locus_tag="LK889_002066"
     CDS             3778..3945
                     /locus_tag="LK889_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBK5096277.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(4007..5818)
                     /locus_tag="LK889_002067"
     CDS             complement(4007..5818)
                     /locus_tag="LK889_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBK5096278.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5815..6180)
                     /locus_tag="LK889_002068"
     CDS             complement(5815..6180)
                     /locus_tag="LK889_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBK5096279.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6343..6738)
                     /locus_tag="LK889_002069"
     CDS             complement(6343..6738)
                     /locus_tag="LK889_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK5096280.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(6756..7718)
                     /locus_tag="LK889_002070"
     CDS             complement(6756..7718)
                     /locus_tag="LK889_002070"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBK5096281.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(7718..7930)
                     /locus_tag="LK889_002071"
     CDS             complement(7718..7930)
                     /locus_tag="LK889_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBK5096282.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(7951..8649)
                     /locus_tag="LK889_002072"
     CDS             complement(7951..8649)
                     /locus_tag="LK889_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK5096283.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(8669..9211)
                     /locus_tag="LK889_002073"
     CDS             complement(8669..9211)
                     /locus_tag="LK889_002073"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK5096284.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(9343..10347)
                     /locus_tag="LK889_002074"
     CDS             complement(9343..10347)
                     /locus_tag="LK889_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK5096285.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(10344..11333)
                     /locus_tag="LK889_002075"
     CDS             complement(10344..11333)
                     /locus_tag="LK889_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK5096286.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(11330..12136)
                     /locus_tag="LK889_002076"
     CDS             complement(11330..12136)
                     /locus_tag="LK889_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096287.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12302..13258
                     /locus_tag="LK889_002077"
     CDS             12302..13258
                     /locus_tag="LK889_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5096288.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(13335..13853)
                     /locus_tag="LK889_002078"
     CDS             complement(13335..13853)
                     /locus_tag="LK889_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5096289.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(13941..14090)
                     /gene="rpmG"
                     /locus_tag="LK889_002079"
     CDS             complement(13941..14090)
                     /gene="rpmG"
                     /locus_tag="LK889_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5096290.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            14318..14764
                     /locus_tag="LK889_002080"
     CDS             14318..14764
                     /locus_tag="LK889_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5096291.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(14959..16854)
                     /gene="asnB"
                     /locus_tag="LK889_002081"
     CDS             complement(14959..16854)
                     /gene="asnB"
                     /locus_tag="LK889_002081"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBK5096292.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(17179..18153)
                     /gene="bsh"
                     /locus_tag="LK889_002082"
     CDS             complement(17179..18153)
                     /gene="bsh"
                     /locus_tag="LK889_002082"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK5096293.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         5654 a         3931 c         3213 g         5905 t
ORIGIN      
        1 gctgcggtat cactccattt tcccacagct tttccatctt ttatttcgtt gattctcgaa
       61 taatcaactc tgtttccaca ataatctttc ttgaaaccgg tgctttggaa ttacttaatt
      121 gaagaatcgt ctccactgct agctctccca tccattcggt ataaactttt acagtagaaa
      181 gcgagggcgt tacatatttc gctacactga catcattgaa gcccatcaca gaaatatctt
      241 ctggtaccac taaacctttt tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat
      301 tagctgcaaa aagcgcatca ggaacatgcg gattcgtttt taaatactgc ttcactgctt
      361 gatatcctga ctcgacagaa aaatctgctt cgataattgg accttcgtcg tagccttttt
      421 ctgccaaaac ttgttgaaat gccataattc tgggatcttt tagccgctga tggtttttct
      481 tcgtatattc tgttccagca agcatagcga tatgttgcgc tttatgggag acaaagtgtt
      541 caatgacttt ttgaacactt gacttaaagt cgacaactag agagtcgatt ccttgagaag
      601 tgccgtcaga atctaccaaa agcaaggcat tttgggatcc tttcatttct aataactgag
      661 cttcgtcgaa ttttcctagt gcgatcgttc catctgccgg aagatcacta atggaatccc
      721 acgtttcttt caataaagta acgtttgctt cttctgcctt tttttcgatt cctaggcgaa
      781 tagataaata ataaagatct tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa
      841 gcgtttgctt agtatttgct gcatttttac gatgtttcgt ataatttaag cgttctgctg
      901 cttcaaaaat tttttgcttt gtggctaagc caacggaaag atcaggatca tagttgagaa
      961 ctcttgaaac agtagcagga gaaactcctg ccaactgcgc aatgtcttta attgttgcca
     1021 tttgtctctc ctcctttttt acgtttataa aatcgcagta aatcgttcaa acgctgcctg
     1081 tcctttttca tctcgcttga atactccagc gtcttccagt acacgtgcaa aaacctgacc
     1141 tacagatttt tgtacgacat cggtgaccgt atctttcgtc aaatcaggat gttctttttt
     1201 taattgttct gcccacggac gatgatattc agccatctgg ttttcttctc ctaataagaa
     1261 atgctctact tctactagtt ctggtttcaa acgaggtggt aaaacagcaa gccccattac
     1321 ttcgatcaac ccaatgttct cctttttgat atgttggaca tctttatgtg gatgaaaaat
     1381 cccatcagga tgttcttctg aaacattgtt gtctcgaagg acaaggtcca actcaaagta
     1441 attgtccctt cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa
     1501 agctctgata gatactgttt catccgaata tgctttccat ttttccaata ctgagttagc
     1561 tgcttcaata agcaagtttt tctctgttga ttgcaaacgg atcacagaca tcggccattt
     1621 cacgactcct gcgatgactt gcggataatc ttctagtttg ataagttttt ctatcggtgc
     1681 ttttgccata ggaaattcat gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc
     1741 tccaacaatt ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat
     1801 ccgaagcagt ctagcaaatg tttcctttgt gattttcatc gggcggtgtt gttctgataa
     1861 aatgatcgaa tgttcattgt aataagcata aggggaatat tggaatcccc agctttcacc
     1921 gtctagattc attcgaataa tccggtgatt ggtgcgggct ggatagttca agcgacctct
     1981 atatccttcg ttttccatgc aaagcatgca ggtaggatag tctactttta ccgcctcgcg
     2041 ttctgctgcg atttgttttg gatctttttc tggcttagat aaattgattg tgatttccag
     2101 ttcaccatac tccgactctg ccggaaattt gatgtttttt gcgatggctc ttgttttgat
     2161 gtagtcattt ttcttactta aattaaagaa atactcagtt gcttcctctg ggcttttcga
     2221 atagtgctgt gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa aatccattaa
     2281 ttgcgcttca aaaatctctt gttctgctgg caattctgaa agaacaccat tttctcttgc
     2341 cttagcgaca agctgatcca ataagtctac agattcttcc ttaactggtc ttacttcaat
     2401 gctttctaga gcatcttctc ctaccatccc taaaagacga ttctgtaaat ataagcggtc
     2461 catttccatc catccgccag cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact
     2521 gattgacata tctcattccc ctattcttca taaccatttg gatggctgac atgccaatcc
     2581 catgctgttt tgataatcgc tttgatatct gtgtattcag gtttccagcc cagaatctct
     2641 ctcgcttttt cacttgaagc gactaatcgg ctaggatctc cagctcttct tggtgctatt
     2701 tctgctggaa tttcttttcc tgtcacttct ctagctgctt ctagcatttc cttgactgag
     2761 tatcctttgc tgctgcctaa attgaagaag ttgctttcat ttccttcttt caagtattcc
     2821 aaagctaaaa tatgtgcagc aatcaaatct tctacttgaa cataatctct gatacatgtt
     2881 ccatctggtg tatcataatc gtctccataa acagccaagg cttttctttg accaagagct
     2941 acttgtaaaa tgattgggac aagatgcgtt tctggtgtat gatcttctcc gatagatgca
     3001 tctgcttttg ctccggctac attgaagtag cgcaaagcga catagcgcat gccatatgct
     3061 tggtcacacc acttcatcat tttttccatc atcaatttac tttcaccata cggattttta
     3121 ggattagctg gtgtatcttc tgtgattgga gactctttcg gctctccata agttgctgca
     3181 gtagaagaga atacgatttt attcacgttg aactcgtgca tgacttcaag taagatttgc
     3241 ataccgtata cgttgttgtt gaaatacatc aatggttttt cgacagattc tcctactaaa
     3301 gagcttgcag caaaatggat tactccttca atcggttcct tttcaaatac ggaacgtaga
     3361 aattctttat cccggatgtc tccttcataa aaatgagcat ctggatgtac tgcttgtttg
     3421 tgacctgtga gcaaattatc taccacagct acttgatacc ccttttgaac tagctggtct
     3481 acagcgtgcg aaccgatata accggctccg cctagtacta aaattgtcat aaaaaacact
     3541 cctttgatta gtaaaattgt gtttgttgat tctatataat ttaaaaaacg aagtttactt
     3601 ttatggtgaa aacaaacaaa gtaaaatttc gtaaattcat atggtaaatt tataataaaa
     3661 catttagtaa acattttcaa ggattttttg caaaggttta caaacaaacg taaaactagc
     3721 ctttttttct cttttgttcc ataatagata taacaaataa taaatgaggt ggattcaatg
     3781 aaaaaatttg tatctggaat attagtagga agtctggcaa cagccgctgc cgttgctggt
     3841 ttggttgctt ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga agcaatgatc
     3901 gaagaaaacc gtaaaaaagc aatgcgtaaa cgcgtttctc gttaataatt atttaaaaat
     3961 acgcatgaag actttctaaa aaacagataa gtcatcatgc gtatttttat ttgtcagtca
     4021 atataagttg tgctatttta tctgcctgtc tcagattcca tggatctgta taaggtctag
     4081 ctactgctgc agttagccaa tccacacctt caacaggtat tccatagcaa taaaagtcag
     4141 actggatttc ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca tctatagctt
     4201 ctgaatgata acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct
     4261 tcaaattttt tatgacagga ttacttgaac gagaaaagct tgtttgtggc aatcgagctt
     4321 caatcaaata gcgactgctg atttctttat cttgtagaga aataacatag cggttgtgct
     4381 tttgctgaat atcaaatggc ggttcgatca aatgaaaaat acccgcatct atcagtgctg
     4441 ctaattcttt cgtccgaatg attggcggac cgatcgttaa aaagctattt agatgagtaa
     4501 accatcccca tagtatttct ttgtattcct tagcagaaaa aacgtgccat tcaatagcct
     4561 gccggattgg atcacgccag tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc
     4621 aattgccttt ttccgcttct tttatctgcc cttcaataaa tattcggcta gcttctgaaa
     4681 agggctcatt ctgataggca agaggagttt ctaaatacga ccaatcccat ctataaggga
     4741 ttagtgctgg atgctttccg agacactcct ctgaagagag ggagagaaaa tcatgctcaa
     4801 aatttttctt tggaatcggc aagtgatatt cttcgatgat tttcttgtaa taaaacaact
     4861 caacgtcttt tttcagatag tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc
     4921 attgctccag gttttctttt gttagtaacc gtggccatgc cattgctccg ccttgctttt
     4981 gatttcttcc tcgagggtaa taagggagcc cccttcttga tccactgtat acaatcggtt
     5041 ccttgcctga tggatgatag accagttttc ccttttcgtt ctcgaagcgc cctcctcttg
     5101 atactgtaaa caatccgaca taatcaaaaa aacttaagcc tagacctctt aaaaagacat
     5161 tttctttagg cggtatcttc gaaagatcta catctgcagg attagaagga agctgataca
     5221 gtaaaacatt tttctttgcg tagtctaccc acactctttc ttcttctgtc tcatcatttt
     5281 cccagtgacc gcttgctaaa acaagctgat cgatagaata gctttgctga tctgtctgta
     5341 aaagataacc atcttttctt ttgcttactt tttttactaa agaataaaca aactgaatag
     5401 aagctggaaa ttcttcttgc aactttgaga aaaaccagcg ttgatagagt ccgtataaac
     5461 atcgagtaga ttgctgatct ttttccagac gattgatttc ttctataatt agttgcttat
     5521 ttttggttac cgtctgttcg atataatctg tagcaggtcc tttactccat tgaaacaagt
     5581 tgggtccctg tgttaccaat cctccactgg acaatgtttc atctgtaaaa agcgtcactt
     5641 gttgagaaac agaattcatt aataactccg gtggctgatt caaccgccaa actcgacctc
     5701 caggaccatt gggatcgaaa agaaaaagtt caagagaatg tctgtcctga ttattatgga
     5761 gaatcctttc tgctacactt aatcctctag gtccggcacc aatgatccct attttcattt
     5821 gcatgcctcc atttcgataa attttttcaa taaaacagca tggttctgat gttcattttt
     5881 tgcaccataa acaaaagtaa tgactggatg ttctttcata agttgactga gtttaaaaat
     5941 agcttgcttc ttttcttctt cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc
     6001 aaatcgttct ggtacatggt caaaagtttt gcgtgcttca ttactaggtg caatttcttt
     6061 caaccacaga tctaactgcg ctttttcttt tgaaattcct cgtggccaga atctatccac
     6121 gagtattcta tagccatcct ctttttcagc atcaaggtaa acacgtttta atttgatcat
     6181 agttgtttcc ttctttctac cttttcgctt agcttatata tgtagataat agcacaaaat
     6241 aaaaaaaatg aacggcacaa ctatccattg cgccgttcat gtgatcttgg tcaaaatcta
     6301 atgattgatt cggtttttaa agctgtcata caatcatctg ttttatactt ttttactttt
     6361 gatttttcca gaccatccgt catatccacc cttaagtaaa taaatctctt gataacctgc
     6421 tttacgtaat ttatttgctg tacggatgct caaacttttc ttttgatcgt aaatatagac
     6481 aggctggtct tttcgaatag atcctaatga atctttcaaa acagtgtaag ggatgtttct
     6541 tgcacctaaa atatgcccag catcaaacac atctttttcg cgaacatcga ttacttgtgc
     6601 ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac gtttggccat
     6661 tactcgtaga tagacttccc aaaggatgat tgcaagcaca atcaatagca aaatgatatt
     6721 aattacccat aaaaccatgt aatgcctccg tttttttaag caaatttcaa ggctaatgag
     6781 gcagcaccaa tgacaccagc ttcattacca agctctgcta atttgatttt tgttgattct
     6841 cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta cacggctacg taaaaattct
     6901 cctgctgccg atactcctcc acctaacacg atacttgacg gatttaatgt attccccaag
     6961 tttccgcaag ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga
     7021 tcgtcttctt gagccatttc aaagatatct ttacttgtta cttcttctcc gttgtctagc
     7081 attgctttca gttttgaatc ccctgcgtat tcttctgcca agaaacgacc taggcgtacc
     7141 acacctgtag cgctagatac agtttctaaa catccgcgtt taccgcaagt acattcgaac
     7201 ccttcaggat ccacagtgat atgtccgatt tctcctccag ctccgctcac gccgtgaatc
     7261 aaatttccgc cagcaacgat acctccgcca acacctgttc ctagagtaac gaatacaacg
     7321 tctggatcgt tttctccagc acctttccat cgttcaccta aagcagcaac attcgcgtca
     7381 ttatcgatag cgaatttgat tcctgttcct gcctcgatca gttttttcac aaattgaact
     7441 tctgtccagt ttaagttata cgcaccaata accgttccag cttcgctgtc cacgcttcct
     7501 ggggtaccca tgccgattcc gataaagtct tcggctttca tattatataa attcaaacga
     7561 tgattgattg attcaatgat ttcaggaacg atatgcttac catcatccaa aatattggta
     7621 tcgatgctcc atttttgctg aatctctcct tctggtgtta aaattgcaaa ttttgctgtt
     7681 gttccaccta aatcaatccc aataatcttc ttgtccatta caaatttcct ttctttcggc
     7741 tttcttcaat tcggtgctct ctttttaata tgcgccacgc tgtcatgtat gtatcatgat
     7801 caatcaaacg gctatcatac agattacgca attcgatagt cattaattca atgtcataga
     7861 tgcgtgcacc cacatagata aagatgccaa attttttgaa cagctgctgt acatcatata
     7921 agctctccat ctttaagcac ttccttcata ttatacaagt aaaccatatt tttttaaacc
     7981 cagtaaaata catattacaa aaagaaatac aaaaactagt gtagaaagaa tcctttggtg
     8041 tatcgaatag cttgtctgtt gtttcggtag acccaaaaca ttccctaaca ctaatccgcc
     8101 aattagccca ccgacatgtc cccaaacatc tacagaggta tccagaagac caaaaacaaa
     8161 tgtcaacaca ataaataaaa gaaaatgtct gactagctgc tggatcgctg ggttatgttt
     8221 aaaatgaaac cctaagataa acaacgcacc gaacaaaccg aatatcgaag tacttgcacc
     8281 accagacaga acattcgctt cgttaaaagc aaagcttgct gtatttccca ttatcccgct
     8341 gaacatataa atcaagaaaa aacgccaatg gccatagatt gcctcgattt gctgtcccat
     8401 aaaatacagt atcagtgagt tcattacgaa atgcatcagt ccaaaatgga tgaagattgg
     8461 tgtcactaaa cgccaccatt cattgaagtg aacaagataa ggaccataca ttccggcata
     8521 ataagggatc tcaagtcgcg gaaataattc catcatgagg aagaccgctg tctgtatccc
     8581 taaaaaagca taggtccata acggtcgtct taaccatgca cgcaatttta tttgctgttg
     8641 ataattcatg aaaacctccc atttcatttt agtcaataaa caattgttgg atcttctgat
     8701 caaagctctc cggttgccat tgttcattca attgttcacg aaaaacaagg ctacatgttt
     8761 ttttatgaaa attatttaaa taccggtcgt aataaccacc gccaaaaccg attcgataac
     8821 cggctctatt gtagataata cctggtacaa tgaggagatc gatcatctcc ggcaaataga
     8881 gcttctctga ttgtggctca agaacaccaa aactcgaacg tacaagaacg gactgttcat
     8941 ccatttcata aaacgccatc tgacgtttcg gcaaagtttt aggtacaact attttctttt
     9001 gttctttttc tgctcgttgg ataaccggct gtgtatccag ttcaaattca ttcgataaag
     9061 tcgtccctat cacttttgct gacttccagt actcgctggc gaatagctgt ttatagattc
     9121 gctgttcttt ttcctttttc ttgccacttt tggccagatt tttcagcttt tcgattcctt
     9181 tccggcgtaa tcttactttc aattggtcca aaaactcacc cttccttctc tcataaaatg
     9241 gtataggaaa aaaagaaaaa tagcaatcga actttcaaat aaatgcataa atagaataaa
     9301 aagaagacct agaaatgatt ggccttcctt ttttaatgaa tttcaactgt tcagcgcgtg
     9361 tttttgtctg ataaaaagtg catacagaaa atatggcgca ccaaaaaggg caatcaaaat
     9421 tcctgtaggt acttccccac ttagtagaat cactctagca accatatctg cactaagcag
     9481 aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag ttattttctt tacgtacgac
     9541 tagcttcgca gcatgaggag ctagtagtcc tacaaaagat aaactacctg tcaccgctac
     9601 actaccgctt gccaaagcaa cagctaacaa caaaaggatt ttttgacttt tagccacgtt
     9661 aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc aatacaatcc tctgtgcata
     9721 aagtcctcct agcggaatta agataaccgc ccatggaagc aatgcccaaa cataaggcca
     9781 gctcgcaccc cagattgatc ccgctaacca tgcattcaca aatccataat tttccttaga
     9841 taattggatc gtgcatagca atgttaatgc agaaaggccg gcattaacgg caataccgga
     9901 caataagatt cgattcatac ccaaaaagcc atttcgatca taggccgcaa gatagacaca
     9961 gcaagcagct aaaacaccac ctagaaaggc tacaactagc aatagaaacg gcagcgaata
    10021 gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct gctccggcat taatgcccaa
    10081 tatactagaa tctgccaatt catttcttgt tactccttgc aagatataac cagaaacgcc
    10141 aagacaagct cctgctaata aactgatcag taatcgagga actcgaaatt ctctaacaat
    10201 cagcgccaaa gaagatgatt ctttccctat gaacgtggca aaaagctgat ccaggtaaat
    10261 aaaaggcgta ccaatcatta aactacctat agacaggatg acaagtacaa aaagcaagaa
    10321 atagaacgtc caatatattt ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac
    10381 ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt caaaaggggg attgatcatc
    10441 ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga
    10501 acataacgat agtccgttcc aaccattccg cgaacaactg gcggaaccaa cagcccaata
    10561 aatgaaacag gtccgactaa cgaaacggca atggctgcta acactgcaac tagacagaaa
    10621 acaatcaacc gcgttcgttt aggatgactt ccaagagcta atgcatgtgt ttcactcaaa
    10681 tgaatcaaag atattgcttt tcctaatgaa aaagaaacca aaacagcaag tatatataaa
    10741 ggaaacaata ctgctaattg tttccatgtg atattggcag ctccgcctac aaaccaaaaa
    10801 gcaagatcct gctgcagatt aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa
    10861 gaactaatag ctacgccagc taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac
    10921 tgcaaaaaac caaatacgag caaagttaca gcaaaagaac ctgccaatga cattagaaac
    10981 acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa gggccattgc caacgaagca
    11041 cccgcgttga ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac tccttgcatc
    11101 aatgcaccgc tcaatgcaaa acaactccct acaagaaatg ctcccgccac acgaggcaaa
    11161 cgaatatttc gtatgatttg gtgactttgt tttgatctat caaaatcaaa caacgcctgc
    11221 caaaaatctg cttttcccac agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc
    11281 aaaagaacaa aacagaccaa aagcaggagt ctatttttct tcatagtcct cataattgat
    11341 cctcctgctc ttttggcaac aaatcatagg ttagaaaaac tggtttattt gttcttggaa
    11401 gcgtgacaat cgttgcatca atctcaaata tttttttcat acactctttt gtgatcactt
    11461 cttcaggtgt accttctgtg atcaacttac cgtttttcat tgctaataaa taatctgaaa
    11521 aacgtgaagc atgattaatg tcatgaatcg acatcaagat cgttgtttgt ttcatttgat
    11581 tgatttcagt caataactgt aggatctcta attggtgtgc cggatctaaa aatgttgtcg
    11641 gttcatccaa gatcaagata tctgtttcct gagcaagcgc catagcgatc cagacacgct
    11701 gggtttgtcc accagacagc gttgcgatat cccgcatacg aaatccagct agacctgtag
    11761 catccaacgc ccactcaatt gcttcatgat cttgcgtgtc catgccactg aacagttttt
    11821 gataagggta tcgtccgtaa gatactactt cttctactgt catacctgta gggtgttcac
    11881 tagtttgtgc taataatgca attttttttg caagttcttt tgaactgtat gtagagatat
    11941 cagagttatt gacaatgatc tttcctgatt tcacaggtag tatacgggta aaacttttca
    12001 ataaagtaga ttttccactt ccatttggcc caatcaaact agtgattttc ccttcaggaa
    12061 tcgtaacaga catgttttca atgatttttt tgttttcgta gccggctgca atgtcagctg
    12121 ctcttaatct cgtcatttca cccatccttt cgctgaaaat cattctcaat tatgacgctg
    12181 tttcaacatt tcgtcaagcg ctactttcat atctatagcc aaatgaagcg gttgacagcg
    12241 ttcgaaatat caggaataat aaccaatgag aaagattctc aattatgtta ggaggaaaga
    12301 aatgaaatta aaaacaaaag tattcacatt atctagcata ttgctgctgg ctggagtact
    12361 gagcgcttgc tccccctctt cagataaaga aacagctaca actgccaatt cgacgacttc
    12421 atccaatagt actcatacag tcacagatac acttggacat aaagtagaga ttccaaacgc
    12481 gcccaaacgg atcatcggca gttacttaga agactatctg attgcactag gggaaaaacc
    12541 tatcgcccag tggactgttg gcagtggttc cattcaagat tatctgcaag atgatttaaa
    12601 ggatgtccca acgatttctt atgaccttcc ttatgagaaa gtgttaagct ttgagcctga
    12661 tttgctgttg atttcttctt ccgctactgt cgaaggcggg aaatacgaac aatatagcaa
    12721 aatcgcgcca acttatgttg taaaaaacgg aacagatgtc acatgggaag aacaactaaa
    12781 agatgtcggc aaagctttgg caaaagaaaa agaggcagaa aaaatcattt ccgattatca
    12841 aaaatccatc aagaaaactc gagaagaact agcagataaa atcgagaaca agacagctgc
    12901 agtgttgtgg gtgaccaaca actcagcctt tatggtcagc aaaaatcggt caagtggaag
    12961 aatcgtatac gacgatctgg aatttggtgt tcctgatcta gtggaagagg tctccaaaaa
    13021 agccacttct gactggtctg ctgtttcctc tgaaaagcta gctgaattag atgctgacta
    13081 catcatcctt gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa gctggaagaa
    13141 tctaaaagca gtgacagaca atcatgtact gcaattcgga cctgaatcta gttggctata
    13201 taacggacct attgccagtc aggaaatggt caatgatatc aaagaaaaac taaattgatt
    13261 ttaactaaaa aaagcatctt cacgattagt cgtgagatgc tttttttctt attaacaaac
    13321 tagacgcata gattttattc ttcttttgaa gcaaccctat tttttactct ttcccatttt
    13381 tgaaaacgat gggcatatag attttcttga tccgttatcc cctcatactc ctctataagg
    13441 gcgaagtctg aaaaatcaat attcccaata tacgtatcgc cttcaaacgt gctatcgatc
    13501 aatgttcgcc agatccgacc agtgtaagga agaagttctt gaaaaatccg actaccaccc
    13561 gaaacataga tatcttctga gatcgtttga ctatacgaca aaagttcatc gatcgaatgc
    13621 attacttccg cattttcttt cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg
    13681 cgataaggaa gtgaaagatc ccccattcct tcataggtct ttcttcccat cactaaaatt
    13741 cgatccattg tgtgttcgcg aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa
    13801 ccatttttgc cgatcagacc atttttatct tgtgcccaca tggaaataaa catattatcc
    13861 gctccttatg attttagatg ttttgagata aaaaaagtct caattctttg accagcaagg
    13921 aattgagaac tttaaaaaca ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt
    13981 gggcaatatt tttgtttttc caaacgatca ggattgttac gtttgttttt gcttgttaga
    14041 tagttacgtt ctttacaaga agtacactct aaagtgatgt ttacgcgcat gtttttccct
    14101 cccattttta caagaatcct ctaagatgac tctcagactt gattatctta ccatgatttt
    14161 ttctagattg ctagtgtttt tcacgatttt gttaagtaaa actacttgac gataaataca
    14221 acggttttac ttctcaaaca aaatcttttt ctttttgtat aaataccctt acacttgttt
    14281 atatagatta tgttttttaa taaaggggga tactaatatg ccatggaaca tgaaggactt
    14341 tcctgcatca atgaaaaact tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa
    14401 tgcattatta gatgaagggt accctgataa ccgtgcgatc ccaattgcca ttgatcaagc
    14461 aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa
    14521 tccaagcaag acggatgaac acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga
    14581 tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc
    14641 aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa
    14701 caaagaatca actttgttca tttataaaaa agatgggaca aaagaaaaag aaatcgatta
    14761 ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac
    14821 aatggtgttc ctctgccaac tccgaaccat aagtcataat atctataaaa cgggaagagc
    14881 tgttttttga aattgtttct tatcactcag tgttaaacgg taggagaaaa cctctttttt
    14941 attgatattt gattttcatc aaacagtcac atagtcgatt gcttctgctt gcggaatcgt
    15001 ttcatctaca aagtagactt tataccatgt caagaaactg aagattgtcc agatttttct
    15061 ttgctcttct ttacgtcctt cgtgatggtc atctagcaac ttcatgattt tttcttgatc
    15121 aaaaaattct tttgcaaaat ctgcttcgaa taatgctttg acttgttcgt agccgtgttc
    15181 ttctttcaac caagctttga ttggcacagg gaatcctaat ttcacacgat tcgaccattc
    15241 ttccggaaga tgtttgttcg cagcttgtct gaaaatatct tttgtgttgt tttgattcaa
    15301 taaaaactta cttggtattt gttgagccaa tttcatgact tcgatatcca ataaaggtac
    15361 tcggacttcc aaagaacttg ccatagaaag tttatctgcc tttaacaaga tatcttttgg
    15421 catccattga tgaagatcga catattgcat cttattgact tcatctttga ttccttctgt
    15481 tttctcgtaa tgaacagtca tgatttcatc cacagatggt gctgtttgat attttggtgt
    15541 taacacttct gatgcttctg attcttcgaa cactcgagca tgaccgataa aatattcttt
    15601 tgcaggagca agtgattcat ataaatggat ccgtccatgg aaatttttca tttttccgat
    15661 tttacgtcct aaattatagc gtgttccttt tggtaatttt ttcaatcctt gagcaatcac
    15721 acggatgaat tttgaattcg tatggaatcc atacgtttgg tatcccgcaa acagttcatc
    15781 cgccccttct ccagattgga ctacccgcac actttgtgaa gcaagatttg ctaaaaagta
    15841 caaaggtacg caagaaggat ttgaatcagg ttcatctaag tgatactgga tcaaagggaa
    15901 tgctttgaac gacatgtctc cgtcgatcac tgctgctgta ttatctaagt ccaatagttc
    15961 ggttagtttt ctggcttcaa tcgcttcatt ataggtttta tcatcaaacc cgattgagaa
    16021 agaatgatct ggtttcaata cagaggttac ataactggaa tcaaccccgc tggataagaa
    16081 agaaccaact tctacatcac tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc
    16141 gattttttcg atccattctt gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca
    16201 atattgctga atgtctattt ttccatcttt atacgtataa taatggcctt caggcaaacg
    16261 atagacatct ttgaagaatg tttcgccgtt taatggagaa tattggaaag tcatataagg
    16321 tttcaacgct tctttgttca attctttgtt aaaatcagga tgaggcagaa aacttttgat
    16381 ttctgaacca aacataaatg ttccgttcat ttctgcataa taatatggtt tgatcccaaa
    16441 gtggtctctt gcaccgaata actcattctt ttcattatcc caaatagcaa aagcaaacat
    16501 tccgcggatc ttttgcaata attctgttcc ccattcttcg taaccatgca aaaggacttc
    16561 tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc aattcttctc tcaaaggctg
    16621 gtaattatag atttcgccat taaaaataat gatctttgtc cgatcttcat tgtaaatagg
    16681 ctgagtaccg ccctctaagt caataatact taatcttctg aatcctaaag caacatgttt
    16741 atcgatatat tctcctgaac tattaggtcc acggtgaacg attcgatcca tcattgtatt
    16801 aataattgtt tttttgttgt ccttgtcgtt aacaaaacct acaataccgc acatttaact
    16861 ttcacatcct aatataaaat tactgtttat ttcattcata tgcatcttat catagaataa
    16921 atagcggacg aatattttct ctcttacaat ttcgtcacgt gaaaacaaaa gatgaaagtt
    16981 tctgttttta cagaaaataa agtcccaata tctaatttac catgttttct tttttgaggc
    17041 taccttattc tcttcgtgtt aaaaaaatat tagtggatgc tatttccaaa aaaaccagca
    17101 aaaaaagaca cgacctaaag atcgtgtcta attatgactg tatcagaaaa tatgatacca
    17161 aacggatcta ttcattaatt aattgacata gtttatctgt tgctctttta tgactggata
    17221 actgaccaat tcatgacgat ccaagtcttc tttattcata tttacagcag taatttgact
    17281 gtcttcatac gtacgataat aataaatccc tttatcaacg ttacagcaag aagaataaat
    17341 cgtatattcg tattttcttt ctccagtatc gcatactcct ttttgttgct cgactgagcc
    17401 tagtatatga aaaaattgac tgatactttc agattctgaa tcacctgatg cagcattcat
    17461 tttagtaaat gtggctttta caaatcgaga aacagaagag agatctccag gtaagcccaa
    17521 tcctcccatc ccacgactat aaacattcaa gctgatctgg tttgaaaaat gattttctgg
    17581 tgttccattt gatagcgagc gataattgtt cagattaaat aattgataat caaatggcgg
    17641 attatttgtc aacacaccga caggattatc atataaatga agtccatctt tcatactttc
    17701 aattactatc gatttttctt tatcagctaa taaccagtgt aaaggtgaca agggtagttt
    17761 ttcactaaaa tcgatattga ctagattgat agtagccaat aattctttag cttcgtctat
    17821 ggttgagcat tgccctagta tccatggaat aaactcaaat ggtgcaacgt tatcttttcc
    17881 ttcttgtaat tctttataat cagcattgcc aggaaaattc agtcctgcca tactcaaccc
    17941 tttttcattt gtagcctcat aatagagtgg atagttggat accccagctg ctattcctat
    18001 cattgcataa tgtttatcta aattttttac ctttctaaaa tcgaaacgat aatttcttgg
    18061 agtaatagtg accacttcat tataagacat ctcataatca aaattccttc caaaataatg
    18121 gtctttcgtt gcataagtga ttgcggtaca catcgttccc cgtcctaaaa caaatcgttt
    18181 ttacactttt atcttacatc tagtattttt tttatcaaaa aaaggccaaa gaagattgat
    18241 ttactagcat ttataaaaaa tagtactcag ggggaatcaa aaaatattgt aggttaaaag
    18301 tacaacaata aggggctgtg acaaaagtct tgtcacagcc ccttattccg aacaaacggt
    18361 gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaac gaaaaaatag gaaaagctat
    18421 ttttgtttgg ccgttcttat tacttgatgc aggacagctt cttcacaacc tctaatcgtt
    18481 gatataagga aggtacagga tttgaacctg cgcgccggta ttagccggtt cgccggattt
    18541 cgagaatagg gtttaaagct gattttaagt gtttttcgct tattatcagc tttttattat
    18601 ctaaaaaaac aacttttaga gacatccata tactgcttgg gggagtcaaa aggggagtca
    18661 tttttatatt ttattcgcaa tttaaatcac ttagagtatg tac
//
LOCUS       DAFBQW010000052        18699 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFBQW010000052 DAFBQW010000000
VERSION     DAFBQW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.053"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            161..994
                     /locus_tag="LK889_002083"
     CDS             161..994
                     /locus_tag="LK889_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK5096294.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            1010..1747
                     /locus_tag="LK889_002084"
     CDS             1010..1747
                     /locus_tag="LK889_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBK5096295.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1902..2870)
                     /locus_tag="LK889_002085"
     CDS             complement(1902..2870)
                     /locus_tag="LK889_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK5096296.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            3102..3935
                     /locus_tag="LK889_002086"
     CDS             3102..3935
                     /locus_tag="LK889_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK5096297.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3969..4808
                     /locus_tag="LK889_002087"
     CDS             3969..4808
                     /locus_tag="LK889_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5096298.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4834..5394
                     /locus_tag="LK889_002088"
     CDS             4834..5394
                     /locus_tag="LK889_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK5096299.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5593..7314
                     /locus_tag="LK889_002089"
     CDS             5593..7314
                     /locus_tag="LK889_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBK5096300.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7479..8621
                     /locus_tag="LK889_002090"
     CDS             7479..8621
                     /locus_tag="LK889_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5096301.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8637..9848
                     /gene="thiI"
                     /locus_tag="LK889_002091"
     CDS             8637..9848
                     /gene="thiI"
                     /locus_tag="LK889_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK5096302.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            10421..11068
                     /locus_tag="LK889_002092"
     CDS             10421..11068
                     /locus_tag="LK889_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK5096303.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    11197..11413
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11461..14106
                     /locus_tag="LK889_002093"
     CDS             11461..14106
                     /locus_tag="LK889_002093"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK5096304.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            14416..15729
                     /locus_tag="LK889_002094"
     CDS             14416..15729
                     /locus_tag="LK889_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK5096305.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            15719..16372
                     /locus_tag="LK889_002095"
     CDS             15719..16372
                     /locus_tag="LK889_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5096306.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            16427..17098
                     /gene="radC"
                     /locus_tag="LK889_002096"
     CDS             16427..17098
                     /gene="radC"
                     /locus_tag="LK889_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBK5096307.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(16993..17667)
                     /locus_tag="LK889_002097"
                     /pseudo
     CDS             complement(16993..17667)
                     /locus_tag="LK889_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            17762..>18699
                     /locus_tag="LK889_002098"
     CDS             17762..>18699
                     /locus_tag="LK889_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5096308.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTK"
BASE COUNT         6282 a         3170 c         3889 g         5358 t
ORIGIN      
        1 gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg
       61 tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg
      121 atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt
      181 atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac
      241 ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa
      301 aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc
      361 ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga
      421 gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac
      481 agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc
      541 ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt
      601 ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga
      661 acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct
      721 gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt
      781 ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc
      841 ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact
      901 gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg
      961 ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa
     1021 atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt
     1081 tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag
     1141 aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga
     1201 gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta
     1261 caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt
     1321 caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa
     1381 tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa
     1441 aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga
     1501 tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata
     1561 tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga
     1621 gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac
     1681 aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa
     1741 taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg
     1801 aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt
     1861 cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa
     1921 atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt
     1981 tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct
     2041 tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg
     2101 attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca
     2161 cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc
     2221 ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc
     2281 agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg
     2341 atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg
     2401 tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct
     2461 tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca
     2521 ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca
     2581 agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata
     2641 cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg
     2701 tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg
     2761 atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt
     2821 tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact
     2881 tccttacgaa taattttgca tagccgttaa atcttattgt atactatata aaagagtttt
     2941 gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa
     3001 tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac
     3061 aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa
     3121 catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg
     3181 cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct
     3241 cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc
     3301 aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg
     3361 cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag
     3421 tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta
     3481 gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg
     3541 atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca
     3601 tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga
     3661 actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag
     3721 agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac
     3781 atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt
     3841 ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag
     3901 atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg
     3961 cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt
     4021 caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat
     4081 gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat
     4141 tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg
     4201 accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc
     4261 gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac
     4321 aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga
     4381 tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag
     4441 agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa
     4501 agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata
     4561 tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa
     4621 ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag
     4681 gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact
     4741 ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga
     4801 tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa
     4861 gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca
     4921 gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca
     4981 gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta
     5041 gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa
     5101 caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc attactagga
     5161 atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca
     5221 gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag
     5281 atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg
     5341 attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag
     5401 tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt
     5461 gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat
     5521 tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg
     5581 gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc
     5641 gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat
     5701 gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat
     5761 gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa
     5821 tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa
     5881 aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct
     5941 acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg
     6001 gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg
     6061 aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa
     6121 cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac
     6181 cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct
     6241 ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt
     6301 gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa
     6361 gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt
     6421 gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat
     6481 tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa
     6541 tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt attgattgag
     6601 ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt
     6661 ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt
     6721 caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta
     6781 gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc
     6841 gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt
     6901 tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt
     6961 gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa
     7021 gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca
     7081 catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat
     7141 cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa
     7201 gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct
     7261 ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa
     7321 aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta
     7381 tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg
     7441 ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg
     7501 caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta
     7561 ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag
     7621 caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag
     7681 gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg
     7741 ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat
     7801 taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg
     7861 tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga
     7921 ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc
     7981 ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat
     8041 ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg
     8101 gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag
     8161 ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta
     8221 aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa
     8281 aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa
     8341 ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg
     8401 tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta
     8461 aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg
     8521 ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta
     8581 ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga
     8641 aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa
     8701 gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca
     8761 aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag
     8821 ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag
     8881 aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg
     8941 ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta
     9001 atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc
     9061 aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat
     9121 cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc
     9181 ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag
     9241 tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa
     9301 aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag
     9361 tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga
     9421 caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt
     9481 tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg
     9541 tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac
     9601 ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg
     9661 aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg
     9721 tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct
     9781 tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat
     9841 tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa
     9901 aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc
     9961 atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta
    10021 tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc
    10081 gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt
    10141 tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta
    10201 tcagaggttg cgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagt
    10261 tcctcatatt ttacgtttgg ttgagcttat taccgcagat gcaagctttt gaacaccgtt
    10321 ttatttgtct cacacccttt tccttcctac ttgtaacctt gctcccgtgt tagaaacatg
    10381 ttacactaaa tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa aaacagatac
    10441 actgccaaaa gcaactgcga aaagattacc tttatattta cgctacttga agatgctgga
    10501 tgactcaggt ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc aaattccttc
    10561 tgcaacgatc cgcagagact tctcgcaatt aggcgagcta ggcagaagcg gatatggcta
    10621 tgatgtccct tttcttatcg atgtattcaa taatatcctt aatactaaag aagaaaaacg
    10681 aatcgcatta gtaggatatg gaaatcttgg caaagcattg aaacacaaca attttagaag
    10741 aaatgaaaat ctaaatatcg tgtgcgtctt tgataatgat ccagcactga tcaatcgagt
    10801 aattgatgga gaaatgatct atccgattga tcgtttcgcg gagatagcca aagcaaaaaa
    10861 tgtgacagta gcaatctcta cggtaccaag taaatattca caatctgcta ttgatgaaat
    10921 tgtcaaagga aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa ctgtcccagc
    10981 ttatgtcaat gttcaataca ttgatttgac aaccgagttg cagacgctta tttactttga
    11041 tgagaattac tcagaagttt tttcttagga aaatttgagt aggcttattg atcttgcgag
    11101 aactcgtcgt aattctttca aaaatttttt agtactaaat gaagtggttt tttcacgacc
    11161 acttgttgac taaaaaaaaa aaccattgta gaatgaagca taacgctgaa caagaggagt
    11221 aactggcaca ttcattctta gagagagaac tatctgctga aaggttctca atgacaacca
    11281 gtgaaggtag cttgggagtt tctgtcttga atggagtaga gatagacgag tcgtgatcgt
    11341 taactcactt gagaggcaat aatgattgat tattgcaatt taggtggtac cgcgtaaatc
    11401 atacgtccta gaatacgaaa gtattttagg acgtttttta gtttaaagga ggaaaacagc
    11461 atgacggaag aaaataacct atcaacaaaa tataatccgc aagaagttga agctggacgt
    11521 tatcagcatt ggttagaaca agatttgttt aaaccgagcg gagataaaaa agcaaaacct
    11581 tattcaatcg taatcccgcc cccaaatgtt actggaaaac ttcatttagg gcatgcttgg
    11641 gatacgacct tgcaagatat gctgattcgt caaaagagaa tgcaaggata tgatacttta
    11701 tggctgcctg gtatggacca tgcaggaatc gcgacacaag caaaagtaga agcgaagctt
    11761 agagagcaag ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga tcaagtgtgg
    11821 gaatggaaag aagagtatgc gagccacatc cgcgaacagt gggctaaaat gggcttatct
    11881 ttagattaca gtagggaacg ttttacatta gatgatggat tatctgaagc agtacgtaaa
    11941 gtttttgtta ccttatacga aaaaggtttg atttatcgtg gtgaatatat catcaactgg
    12001 gatcctcaag cacgaacagc tctatctgat atcgaagtta ttcataaaga tatcgaaggc
    12061 gccttttatc atatgagcta tgaactagca gatggatcag gcgtagtaga aattgcgaca
    12121 acccgtccag aaacgatgtt gggagatacg gcgattgctg ttcatccaga agacgagcgt
    12181 tatcaagcgc tcatcggcaa aaaagtgatt ctacctttag tcaataaaca aatcccaatc
    12241 attgcagatg aatatgtaga tatggaattc gggacaggtg tagtaaaaat cactccagca
    12301 catgatccaa acgactttga ggtaggtaat cgtcatgatc ttccacgcgt aaatgttatg
    12361 gatgatgatg gtacgatgaa tgaactagca ggaaaatatg ctggaatgga acgttttgct
    12421 gctcgtaaag caatcgtgca agatctgaaa gaaatcggtc gattgattaa aatcgatacg
    12481 atgatccaca gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc tcggctgtct
    12541 actcaatggt ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa ccaagaaaca
    12601 gatcaagcag tggaattctt ccctccaaga ttcaatcaaa cgtttttacg ttggatggaa
    12661 aatgtccatg attgggtaat ctcacgacaa ttatggtggg gacatcaaat tcctgcatgg
    12721 tatcacaaag aaacaggtga aatgtatgta gggatggaag caccagcgga tagtgaaaac
    12781 tgggtacaag atcctgatgt tttagacact tggtttagtt cagctttgtg gcctttctca
    12841 acaatgggct ggccagatac ggaaagcgaa gattataagc gatacttccc tactagcact
    12901 ttagtaaccg gttatgacat cattttcttc tgggtaagca gaatgatttt ccaaagcttg
    12961 gaatttactg gcgaacgtcc atttgagaat gtcttgattc atggattgat tcgtgacgaa
    13021 caagggcgga agatgagtaa atcattaggt aatggaatcg atccgatgga agtaatcgaa
    13081 aagtatggag cagatgctct ccgctggttc ttatcgaatg gttctgcacc aggacaagat
    13141 gtccgcttca gctatgaaaa aatggatgct tcttggaact ttatcaacaa aatctggaat
    13201 gcttcacgtt tcgttatcat gaatatcgaa ggaatgaccg tagaagagat cgatttatct
    13261 ggagaaaaat cagtggctga ccggtggatc cttactcgtt tgaatgaaac gatcgaacga
    13321 gtaactgagc tatttgatcg ttttgaattt ggtgaagcag gtcgacaact gtataacttt
    13381 atctgggatg atttttgtga ctggtacatc gaaatgagta aagaagtact ttatggagat
    13441 gatgctgttt caaaacaaac cacccgtagc atcttagtcc atacacttga tcagatccta
    13501 cgtttattgc atccaatcat gccattcgtt acagaagaaa tatgggaaca tattcctcat
    13561 cagggaaatt ctctagtagt ggcagaatat cctgttgtac gtccagagtt tgatgatgaa
    13621 acagcttcta aagggatgga agtattgaaa gaattgattc gagccgttcg aaatattcgt
    13681 tcagaagtga atacaccatt gtctaaacca attacgttgc taattaaaac aaacgatgct
    13741 aaaatcgatc aatttttagt tgaaaataca agctatatcg aacgcttctg taatcccgaa
    13801 gagttgacga tatcaagtga gatcactgcg ccagatctag cgatgtcagc tgttctgaca
    13861 ggtgcagaaa tcttcttgcc gttagcagga ttgatcaata ttgaagaaga aatcaaacga
    13921 ttagaaaaag aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg aaaactttct
    13981 aatgaacgtt ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg tgcaaaagaa
    14041 aaagattatt tagagaagca aacggctgtc aaagaacgaa tcgaaagttt gcgtactatt
    14101 caataagaat agaaaaagag agtgtgaaat cagctagcta ttactaaaag ctagctgatt
    14161 gaacatcgtt acaggaaaga gtttgagcct cactcttagc ttcgattcat aaggagatgt
    14221 gacataaatt ttgtcgcatc tcttttttag tctactctta ttatgtagga gaaggaggga
    14281 ggtggatcat gtatcaagag tcaataaagt agttgataaa atcgttcctc tctctgtttc
    14341 ttttctcttt gtcatacttt tatttttctt atgtgttcta tttgttataa taaaagagaa
    14401 atgggggaat tgttgatgac gattgaagaa gcaatagaat ggatccatag tcgattacca
    14461 tttggttcac gtcctggcct tgaccgagtc gaagctttat tagaagtggt agggcatcca
    14521 gagagaaaag taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc tactgtagct
    14581 tatttgagaa gcctattaga agaggctggg ctgacagtag gtacatttac ttcaccttat
    14641 atcgaaacgt tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga caatgatttg
    14701 atccagcttg taaaaaaata tcagccactg atcaaacaac ttgatcaatt tgaagcagta
    14761 gctggtatta ctgaattcga gacattaaca gcaatggcat ttgattattt tttgatgaag
    14821 aaagtggacg tagcgattat cgaagttggc ttaggcggct tattggattc taccaatgta
    14881 gctgtaccaa tgctgacggg aataacaacg attggacttg atcataccga aatacttgga
    14941 gatacaattg aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg cgttccagta
    15001 gtaacaggaa atattgcccc agaagctttt actgtcatcc aacaaacagc taaagcaaaa
    15061 aatgcaccgc tttatgtata cggggaaaat tataaaataa actatctgca tcctgatgag
    15121 atgtggggag aagtatttga attttttaat gaaataggaa agatccctca attaaccact
    15181 ccgcttttag ggcaacatca agctgaaaat gcaggagtag ccatcgaatt gtattttgtt
    15241 tactgtcaat tggaaaagct gcctttccga gaaaaagatg ttttgaaggg attgaagaaa
    15301 tctcgatggc caggaagaat ggagcgtttg agtaacgagc cattagttgt tttagatggt
    15361 gcccataata cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga attcaaagga
    15421 tatcaaatca acattttgtt ttccgcctta cgaactaaaa atgttagaga aatgctagaa
    15481 cgattgcagc aagtaccaaa tgtccatatt tatttgacga cctttgaaca tccaaaagcc
    15541 atcgaattaa gtgaagtatc agatttagca agtgagagag tatcaatcgt ttcattgtgg
    15601 caattcggat tagcagatat attggaaaag atgacaaatg aagacgtatt agttgtgacc
    15661 ggttcacttt atttcatttc agaagttcgc catttattaa tacaattagg aggagatcat
    15721 gaagtataat gcagtgattt ttgacatgga tggtttgctg ttcgacacag agttagtgta
    15781 ttacgaagcc acacaagtag tagcagacaa gatgggtttt ccttatgata aagacctgta
    15841 tttaaaattc ttaggtgttt cagatgaaga agtatgggcg aattatcata tgattttctc
    15901 ggaatttggc aaaacaagtg tacaaagatt catagatgat tcataccagg aaacgcttga
    15961 acgtttttcg caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc tctctttttt
    16021 agaagaacat cagattccca aagttgtcgc ttccagtaac caacgagcag tcattgaact
    16081 tttattgaag aagaacaatc tgtttgaacg atttgagacg attgtttctg cagaaaatgt
    16141 tcaacgagca aaaccagacc cagaaatttt cttgttagct aatcagaagc taggtacagc
    16201 gaagaaagaa acattgattt tagaagattc acaaaatggt attttagcag cttctgcggc
    16261 tgagatcccg gtagtcatgg taccagatct gcttccacca tcccaagaat tgatcaaaaa
    16321 aacagctggg gttgtttctt ctttacatga aatccctgca ttgttaaaat aaaaatccat
    16381 ctcttttcgt atcttatttt agataagata gaaaggagat gtttttttgg gaaaacgatt
    16441 gatcaaagaa gtacctacta gctctttacc aagggaacgt atggaaattt atggcgcgga
    16501 agctttatcg gatcaggagc ttttggcaat cttactgcgt accggtcaac acccttatag
    16561 tgtgatgtcg attgctggaa atctgttaaa aacgtttgga ggactggcct ctttacgaca
    16621 ggctactttg catgagttag aagagattcg agggatagga cgcgtgaaag caatcgaaat
    16681 caaagccttg atcgaattag gtcgacgaat tcagacggat catgaaagag cggctccacc
    16741 ggttcgtacc agctacgaat tggcccagca attgattctg gaaatgaaag atcacaaaca
    16801 agaacatttg gtttgtattt atttggacac gaagaatcaa gtgatcttaa aaaaaacagt
    16861 gttcatcggt tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc attatgctgt
    16921 acgttattgt gcagcaagga tcgttttagc gcataatcat ccttccggaa tatataagtt
    16981 agtacacata aattatataa ggaaatttac tgttatattg ccatctggaa atactattat
    17041 gttatcgata attgaacgcc ataatgcatt tttttcttct tccgaagatt ctttataaat
    17101 ttcatcgaat tcttttgata gaataaattc gacatttttt atatctattt tagctggctc
    17161 tacttcaatt tcatttaatt ctttcattaa agcttctcga tcttttttgt attcctctaa
    17221 gtctataact tcattcaagt aggctttctt caatctatct aatttattct caatattttt
    17281 cttactattt ttctttttaa taatctttgg ttgttttttt gaatagttta tttgtatttg
    17341 ttcaaatttt tgtttagtag cttcaaaaat ataacgttct aaattttttt cgtaaaaagc
    17401 gcgcttattt gtgcaacgta atttattatt tcttcgtttt acacatctat aacaagtcct
    17461 ttcgtatata gattctgtac cgtctttttt cttatatctt gctttaatag tttgaccagc
    17521 tactgatccc tgacagtcag aacattttaa taaaccttta aaaatgtaat cacgttttgc
    17581 atttgttttt aaatttttag gtaaggcttt ttgaacagca taaaaaagtt ttttatcaat
    17641 aataggagga cagtaatttt tattattcgc cgattgtaaa attaagctag acaactaaaa
    17701 aaagtcattt gtgctacact caaaatagtt ccgacgaaag aattataagg agtgagtaca
    17761 aatgacctat aaccatctta caccaactga actcgtaatg atagaagctt atttcaatca
    17821 atcacaacct gtttcaaaag tagccaatct gttacaacgt tctcgacaaa ctatttataa
    17881 ggtctatcgt tttcttaaat ctggtggaag cgcaatgagc tactataaac aatataagaa
    17941 aaacaaacgt aactgcggaa gacatccgat tgttttacct gatgagcaac aggaatacat
    18001 tcaaaaaaag gttgttcaag ggtgggctcc tgatgtcctt attggtcgag cagagtttcc
    18061 tattgcttgt tcggtacgca cactgtatcg aatgtttaaa gataaaacct ttgacactca
    18121 cgatttacca atgaagggaa aaagaaaacc aaacggtcat aaagaaaaaa gaggaaaaca
    18181 agggtttcgc cggtcaatcc gagacagaaa gacagactat acccagtttg atcatgaatt
    18241 tggtcacctg gaaggtgaca ctatcgtggg tggaaaacat aaaagcgcgg tcatcacatt
    18301 agttgaacgg ctatctaaag tcatcattac cttgaaaccc gaaggcagac aggctaaaga
    18361 cattgaaaat aggctgaatc aatggtttcg ttgtgtccca aaaaacttat ttaaatccat
    18421 cacatttgat tgtggaaaag aattctcaaa ttggaaaaag attagtaata ccaacgatat
    18481 ttcaatttat tttgcggatc caggaacacc ttctcaacga ggattgaatg aacattcaaa
    18541 tggcttatta cgtaaagatg gtttgtataa agctatggat tttaatggtg tatctgaaac
    18601 ctttatccaa tcggtggcat cgaaacgaaa tcatattcct agaaaatcat tacactatcg
    18661 gacaccaatg gaagtatttt tgagttacgt cacaaaaga
//
LOCUS       DAFBQW010000053        18656 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFBQW010000053 DAFBQW010000000
VERSION     DAFBQW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.054"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     misc_feature    111..236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            280..804
                     /gene="infC"
                     /locus_tag="LK889_002099"
     CDS             280..804
                     /gene="infC"
                     /locus_tag="LK889_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK5096309.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            876..1076
                     /gene="rpmI"
                     /locus_tag="LK889_002100"
     CDS             876..1076
                     /gene="rpmI"
                     /locus_tag="LK889_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK5096310.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1129..1488
                     /gene="rplT"
                     /locus_tag="LK889_002101"
     CDS             1129..1488
                     /gene="rplT"
                     /locus_tag="LK889_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK5096311.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1740..3047
                     /locus_tag="LK889_002102"
     CDS             1740..3047
                     /locus_tag="LK889_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK5096312.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3124..5253
                     /locus_tag="LK889_002103"
     CDS             3124..5253
                     /locus_tag="LK889_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBK5096313.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5228..5917
                     /locus_tag="LK889_002104"
     CDS             5228..5917
                     /locus_tag="LK889_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096314.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(6325..7011)
                     /locus_tag="LK889_002105"
     CDS             complement(6325..7011)
                     /locus_tag="LK889_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5096315.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(7147..7341)
                     /locus_tag="LK889_002106"
     CDS             complement(7147..7341)
                     /locus_tag="LK889_002106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096316.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(7391..7831)
                     /locus_tag="LK889_002107"
     CDS             complement(7391..7831)
                     /locus_tag="LK889_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBK5096317.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(7835..8680)
                     /locus_tag="LK889_002108"
     CDS             complement(7835..8680)
                     /locus_tag="LK889_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBK5096318.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            8829..10247
                     /gene="pepV"
                     /locus_tag="LK889_002109"
     CDS             8829..10247
                     /gene="pepV"
                     /locus_tag="LK889_002109"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBK5096319.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(10304..11251)
                     /locus_tag="LK889_002110"
     CDS             complement(10304..11251)
                     /locus_tag="LK889_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5096320.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11474..11824)
                     /locus_tag="LK889_002111"
     CDS             complement(11474..11824)
                     /locus_tag="LK889_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK5096321.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12024..13103
                     /gene="pepA"
                     /locus_tag="LK889_002112"
     CDS             12024..13103
                     /gene="pepA"
                     /locus_tag="LK889_002112"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBK5096322.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13247..13567
                     /locus_tag="LK889_002113"
     CDS             13247..13567
                     /locus_tag="LK889_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK5096323.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            13589..14053
                     /locus_tag="LK889_002114"
     CDS             13589..14053
                     /locus_tag="LK889_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5096324.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            14258..14863
                     /locus_tag="LK889_002115"
     CDS             14258..14863
                     /locus_tag="LK889_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBK5096325.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(14902..16191)
                     /locus_tag="LK889_002116"
     CDS             complement(14902..16191)
                     /locus_tag="LK889_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBK5096326.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            16619..17098
                     /gene="rlmH"
                     /locus_tag="LK889_002117"
     CDS             16619..17098
                     /gene="rlmH"
                     /locus_tag="LK889_002117"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK5096327.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            17183..17692
                     /locus_tag="LK889_002118"
     CDS             17183..17692
                     /locus_tag="LK889_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBK5096328.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            17792..18442
                     /locus_tag="LK889_002119"
     CDS             17792..18442
                     /locus_tag="LK889_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096329.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
BASE COUNT         5997 a         3373 c         3837 g         5449 t
ORIGIN      
        1 ggaatcactg ctagaaagtc tgctaatcta atcattttca gaaaaggaag acaaactgta
       61 tgaaaaacat tgacaaaaag attagttttt gctatactat gatggttgag aaataaaagc
      121 agaagcaccc gcttctcgcc ttagttgata atgtcactgg gctagatagt tttcactggt
      181 tctattaggt gaagtacgcg cgggttctgt aagagaactc gtttttttat tgccgtttat
      241 tcggtaagct tttctttctc tcataatatt tggaggtgaa tgaccatagc aaaggatatg
      301 atggttaacg acggcattcg tgcacgagag ttacgattga tcggttcaga tggtgagcaa
      361 ttaggagtta agacaaaagc agaagcattg aatattgcag aacaagcaaa cttggatctt
      421 gtgttagttg cccctggtgc gaaaccacca gttgcgcgaa tcatggacta cggtaaatac
      481 cgtttcgaac aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta
      541 aaagaagttc gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa acttcgtaat
      601 gcacgtaagt tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt caaaggccgt
      661 gccattaccc ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg
      721 gatattgcaa cagtggaaca aaaagcgaaa atggacggac gcagcatgtt cttgacgctg
      781 gcaccgaaaa acgacagtaa gtaatttgaa tgactaactg gctgttggac agccgatagt
      841 cggataacga aagattttga ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg
      901 gatcagcaaa acgtttcaaa cgtactggta aaggcggatt gaaacgcttc cgcgcgttta
      961 caagtcaccg tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca
     1021 tggtttctag tggcgacttc aaacgtattc gccaacaact agcaaaaatg aaataagaag
     1081 tacaaataac agaatctgaa gatttatttt tattaggagg aattatacat ggcacgtgtt
     1141 aaaggtggaa cagtaactcg taaacgtcgt aaaagagtgc taaaattagc aaaaggttac
     1201 tacggttcaa aacacacttt atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac
     1261 tacgcatatc gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt
     1321 atcaacgcag cagctcgtat gaatggcttg agctactcaa aattaatgca cggtttgaaa
     1381 gttgctgaaa tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa cgatgcagca
     1441 gcatttactg cattggctga ccaagcaaaa gaagctttgg ctaaataagc taaacaatta
     1501 taaagaggaa agaagataac gaaaagttat gttctttcct ctttttttat ttacaatcct
     1561 ccatcaaatg aaacaggaca ataacgtata agtctaaaat agcataatta aggtttttta
     1621 attgaaaact gaacatttat ggtcaccctt taatgaaagc gctaactaca taagttagaa
     1681 tgagtttgac tgatatatta gtttgcttat cagaaaaaca aaatggacgg aggaataaca
     1741 tgagtgatat tattattggt attttgttag ttatctcatt ctttttcatg gtctggtact
     1801 gtgtaaaagg ctacaacttg atggtcggct ttgccatcat ggctactgta tggatgggat
     1861 tagcgctagt cggaaacacg ttttcaccaa acccggcgat ggaagggcaa ggagtaattg
     1921 atgtgttgac gcatatctat acgacggggc cagctgagta cgccaaatcg atcttggtaa
     1981 atgtcttctt tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc
     2041 tgattcgaaa ggtagttgaa cttggagggg acaagccaag aatcacgatg agcttactat
     2101 gtattgtaac cgctgttata ttcatgtcga tgacaggaat cggaccagtg atttctattg
     2161 cagttattgt gttgccaatc ttgatgtcat taggtatttc agtaccggtt gcactgttct
     2221 catttatggg ctcgattatg gctggaatct ttgctaatat cgtcaatttc aaacaatatc
     2281 aaacgattta tgctggtttc aatccagcgg ctgaaagcta tacttataat gactatttcc
     2341 aaattggtat gattgggatg attgtaagct tagttgtggt actaacggtc gctaatatat
     2401 caatgaacaa aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg
     2461 caccaatgat ctcatggtta gccgtattat taccagtttt aggggtcgtt cttttagatc
     2521 ttccaatcat cttaggtttt attctagcag gtatctgggc attgctgttt actggaaaat
     2581 tgcgcggagg ttataaagaa atctgtcgtc agtttgcgaa attatttaca gatggcgcag
     2641 tagatgttgc accaatggtt ggtttcttga tgaccttagc gatgttcaat aattcagctg
     2701 cgtatgcttc tccttatttt tcagctattt ttggagattg gataccgcaa tcaccattag
     2761 tcttggcaat cgtatttgct atattgacac cactaggatt tttccgcggt ccaatgaatc
     2821 tagtcggttc aggctcagct attttagctg tcgtattagc tgttaatcca acgatgtcgc
     2881 cagccttttt gttcccgtta tttgcaatca caacgattgc tcctcagcat ttagatatta
     2941 cacaatcttg ggttgcttgg ggattaggtt atacaaaagt aacttctcga gaatatatga
     3001 agaaatccat cccaactggt tggatcatcg gagcgatctt tgcctgatca cgttcttatt
     3061 gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag
     3121 aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg gtacacatac gaaagcagtc
     3181 gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat
     3241 gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc aaaattgttt gcgggaaaat
     3301 aatatcagtc cagaagatgt tgtgtttgtt gcacattcta ccacacaagc gactaatgca
     3361 ctgattgaag gagacgtagc aaaagtcggc gtaatcggga tggcaaaagg tggattagaa
     3421 ggttttttag caaaacaaca aactagattg aacgatatcg atttagggaa taagaaaaaa
     3481 atagagattg tcaatgcatt cttacctgtc aaacacttaa atgtagaccg tgtttcagaa
     3541 acgatttctt ctttagaaag agaaagagcg gaggtcttgg tttcttctat ggcatttgga
     3601 gtagataatg gggaaccaga acgagttgtt tatgaagcag catcggtaaa atcgatccct
     3661 acaacgatgg cttctgacat cacaaaatta tatggattga caagaagaac tcggacagca
     3721 gcaatcaatg catctatttt accaaaaatg ctagatacgg caacctctac agaagattcc
     3781 gtacgagaag caggggtcaa tgtttctttg atgattatgc gcggagacgg cggagtaatg
     3841 gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc tttccggtcc agcagcatca
     3901 gtcatgggct ctttgatgta cttgagagcg tctaatggtg tttattttga agtaggcgga
     3961 acaacaacca atatcggggt cattaaaaat ggacgtccag caatcgatta ttcaattgta
     4021 ggtggccacc caacatatat ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt
     4081 tcgatggtac gggctaatca gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt
     4141 gccggtctag attatgcagt atttacggag acagaaaaaa tcaaaggtcc taaggttgaa
     4201 tttttctctc ctaaagaagg agaccctgct gattacgtca aggtagtgat ggaagacgga
     4261 gaagaagtga ctattacaaa tacatgtgca gccaatgtcc ttggattagt gcaagaagaa
     4321 catttttctt atggaaatgt cccatctgca cgaaaagcca tccaagcatt agctgattat
     4381 tgtcatacga cggttgaaga catcgcagaa cagatcatgg aaaaatccta tgctaagatt
     4441 gaaccggtga ttttagaatt ggcagacaag tatcacttgg agaaagatca gatttctctt
     4501 gtaggtgtag ggggcggcgc tgcctcattg attacttact ttagtaataa aatgggtgtg
     4561 aaatattcga ttcctgaaaa cgccgaagtg atttcttcta ttggtgtagc acttgcaatg
     4621 gtccgagatg ttgtagaacg tatcatccct tcgccaagta aagaagatat acgatcgttg
     4681 aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta caccagaatc gatcgaagtc
     4741 cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta tcgctactgg ttctacggaa
     4801 gtgaaagcaa ccgatctaac caaggagatc acaacagaag aagcactaga gcttgctgca
     4861 gaggatatgc gattaaataa aaatgaagtt tgcttattgg aaaatacacc tttcttttat
     4921 gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa
     4981 ggatttatca aggtccaacg tggacatgct tcatgtatga aaacaactgc agccaattat
     5041 atgacagctg tcgaacaact ttgggaagat atggcagttt atcaaactga attgattgcc
     5101 cgaccagaat tttatctctg cttaggagca agagtctctg attttacagc gacggatctg
     5161 gaacaactac agcttttgat ggatctggaa gtatctacta tggaaccgga ggaagaagtg
     5221 atcgtcgttg ccggaaatat caaacaaaca taagttagaa gagttactaa acgaattaca
     5281 acagatcccc gaagaaattt ggggattcta ccagtttcgg cgagatttat tttggaaaaa
     5341 gattcctttg tccaagcaga aaattcttat acaacaatca atcgattgtg gtatcgaaac
     5401 ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta ggagaaattt gtgaacaaat
     5461 ggctgttcca atcgtttcct gtgaatcaga gcaaataaac gaacgaatca cttttgcaac
     5521 ttatgcagaa gatgaaggga ttcgattgat gacggagccg ttagaaaaat taaaatgttc
     5581 aggactagct tcgatctcta aagaaacagc gcaagctttg attattggac atgaactatt
     5641 tcaccacatc gaagcttctg ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg
     5701 gcggttgcct ttttatacac atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat
     5761 gagtttttca aaagagatga atcaaagcga tttttcgcct tatgtattag aggcagtctt
     5821 actttggcca tataatgaaa ttcgtagcca agggatctta gaagaaataa aaaaaataga
     5881 aaaaagatgt acggagtatg atttagatta caaataaaga gactgtgaca aaagtcgtgt
     5941 cacagtcctt tttttccgaa taaacgatgt tctcctgtca actcctcggt cacaagtcaa
     6001 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagatgaa
     6061 cggcaggctc acgacctctt ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt
     6121 ccagccaagt taaaaacata agaagctatt tttacttggc tgttcttatt acttgatgca
     6181 gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat cagctgaccc tcggtattag
     6241 ctcaaaaaaa acggcaaaat atgaggaact attttgccgt ttctttctct aatactcagg
     6301 tcaaaacgct gattccaaaa cctcttattt gatgggtctg tcctgaatat cttcaaaata
     6361 atcggggtaa ttatgaatca cgtattcttg ctcctcgata atcatatgta aatgcatcgt
     6421 gcaaagaaat ccgacttcat caaagttatg atcaatcatt gattgaagca ttgtctgatg
     6481 ttcatctaat actcgacccc agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg
     6541 gttcaagtgc gtactataac tttctagcca atcccaaatt tctcgttttc cagcaatttc
     6601 ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa gcttttttat tttgagcgat
     6661 tactgcctga tcggtttgtt ctagtatttt ttctagttta gcttgatctt gcttggtcat
     6721 tttcgcactg cattcttgca ggatcggttt ttccaacttt tcccgcgtat aacgggctaa
     6781 tgaagcagat cggatatcga tttttgaaac ataagtgccg gactgtggaa tggtttttac
     6841 aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc ggtgtacgcc cgatagctag
     6901 ttgttcttct aacactttat cggagatttt acttccaggt gctaactcgg aataaatgat
     6961 ttgtttacgt atagattcat aagcttgctg ttgcaaatta ttgataacca tttaaccttc
     7021 ctttctatgc agatataatc tattataaca taaatcgctt acaaatcgtg tgggcataaa
     7081 aacacagtct gcaaaaaaag ccctgatagg catgcctatt agagcctcga aaagaaaaat
     7141 attgaatcaa tctggtcgta cctcttgtat cggtttgaca cgaccgccga ttcttttagc
     7201 aaaagcttcg gattcttttt tgtctttgaa aatgagcaga ttgtctaagt ttttttcccg
     7261 agtgcgacag ccagttttat cgatgtaacc gtgcattact ttaacgacat acatggggat
     7321 cacctgcttt tttcttttca ttatacgaac tactcgacac aatacaagaa aaatgcttag
     7381 acggtcttta ttaagaattt acatcatcca aagaagcgta cgcttggaat aactgatcta
     7441 actccggatt gactttgctc agcttcacta atcgttgcga atcagaaagt aaaaaacagt
     7501 gtttactact gccagtagtt tttaattttt tgtcttcagt tcccacaatt tcgtagctaa
     7561 aatccaaccg tgtccccgta tacttttgga tagttggatg gatgctcact ttttctccgt
     7621 aatggatcat ttctttatag ctgcaattta cttccagaac ggggatgatg atcccagatt
     7681 cttcgatttt tttataggga aaccctagat gtaccaataa ggctacccga gcctcttcaa
     7741 accagcggat ataattgctg tgatgaataa tccccatttg atctgtttca taataaaaag
     7801 gttctcgcac ataacctgta aatttctcca tgactcatgc ctcgttttcc attgattttt
     7861 tacaaaaata attcatccat ttcggaagtg cttcgctgat ttgtgtcggc aaaacaatat
     7921 attgttcttt tgccagctgg tctccaatga aactatgaag ataaatagct gctgcaatcg
     7981 tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat catcccagct aaagtgtcac
     8041 ccatgccgcc cgttgccata gcagcagaac ctaaaggatt ggtgtagaag tgctggtgac
     8101 cataaatttt tgtctcatga cttttcaaaa cgagcacagc accgatttcc tgttgcttct
     8161 ttttgttttc tttatcccgt tgttcttgta tcggtagaga actcaaccgt tgccactcca
     8221 tttgatgagg tgtaagtaca gtttgttcgg gatagggtag caaaagatga ttagcagcaa
     8281 gtaaacttaa agcagagcca tcaatcacca gccattgttc ttttgattgg ttttctagta
     8341 cttgtttgaa acgttccaaa ctcttagaag aagtacccaa tccgggacca atcaagagta
     8401 catctgcttg ctttattacg gaaagaaact ggtcagtatc cgtccaaact acaatcattg
     8461 cttctggcag atgactgtga agagcactcc gatttttttc atcagttact acagtcgtga
     8521 gacccgctcc gctgtgtacg gcagctaaag cactcataat gattgctccg ccatattgtt
     8581 cattgccgcc aatcaaaacg atacgtccaa atgttccttt atgtgaaatt tttggacgag
     8641 gtgtgatgat tcttttcact agttgttcgt ttatcagcat ctaaaaacct ccttttactc
     8701 attatagcgt atattttttt tagagaaaaa cagataagaa aaggaattgt cagatttttt
     8761 gaactatttt tttctgtaat ttgtggtatc atgaacacga agacttaaat aagtggagga
     8821 ttaactttat gacaattgat tggcaaaaag aagtagaagc acgtaaagaa gacttgcttg
     8881 aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga tgactcaaaa gtaacaccag
     8941 atgcaccatt tggacctgga cctcgcgacg cattgaaaca tatgttagct tatggcgaac
     9001 gtgatggatt taaagtaaaa aatgtcgata actatgcagg gcatattgat cttggcgaag
     9061 gagacgaaac gctagggata tttggacaca tggatgtcgt tcctgcagga gatggctggg
     9121 acacagatcc ttatgaacca gtaatcaagg atggaaagat ctttgcacgc ggatcaagcg
     9181 atgacaaagg tccaagtatg gcagcttact atgcgatgaa gatcattaaa gaacttgact
     9241 tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga tgaagaaagc ggctggggcg
     9301 atatggctta ttatttcgaa catgaagaag aaccagattt tggtttctca ccagatgctg
     9361 aattcccaat catcaatggt gaaaaaggaa atgtttcttt agctttacgc ttcaaaggcg
     9421 acaatgcagg agattatgtc ttgaaatctt ttgtttccgg acttcgcgaa aatatggtgc
     9481 caggaacagc aactgcagca cttcaagtac caagtgcaga tgcagctatc gcgatggaag
     9541 aagcatttta ccaattcatc gaagcaaatc cagtaagcgg aacgattgaa gcggacaata
     9601 cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg tgcaagccca caatctggta
     9661 tcaatgcagg ttctttcttg gcattgttct tagataacta tgaattccta ggatctgcta
     9721 aacaatttat ccatgtagca gctgcttatg tccatgaaga tttttatgga gaaaaactag
     9781 gagtcgctta tgaagatgaa aaaatgggca aattgacaat gaatgctgga ctatttgctt
     9841 ttgaagaaaa tggtacagag gaagcgaact tcatcaatat gaatttccgt tttccaaaag
     9901 gtgtaacagt agacggattg caaagtgaca tcgaacaaac agtggggcaa gaaggcgcaa
     9961 cagttactcg aggtgctcgc gtaatggaac cacattacgt accaatggat gatccattgg
    10021 tggctacatt attgcaagtc tatgaagacc acactggaga aaaaggctac gaacaaatca
    10081 tcggtggcgg aacttacgga cgtttgttga aacgtggcgt tgcttatgga gcgatgttcc
    10141 caggatatac tgatacaatg catcaagcaa acgaatttat gagtcttgat gacttgttca
    10201 gagcaacagc aatctatgca gatgccatct atcgtttggc gaaataatta attaaaaaga
    10261 ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt tttttacttt ctactgtggt
    10321 attcatgata aacatgatat agattcaatt catattcttt cttagcgttc gtgacgactg
    10381 tatccaaaat caaccctgtt actaatgaaa gaagtgctaa agtcatcaat ccagtactta
    10441 aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat atattcgcga atcacaggaa
    10501 tccccgtgac caatccaaat aaaaagaaga agatggtcca aatcccaaag aaaagcaaag
    10561 gcttataatc tttaaacatt ttaataatca tcatgatcac tttgaatccg tcagagaatg
    10621 tattcaattt tgattcgctg ccttcaggac gatcacgata atcgattggg atttcaacga
    10681 acttgaattt tttatctaaa gcatggatcg tcaattccgt ttcgatttga aaaccagaac
    10741 tcataatagg aaaacttttg acaaagatac gattgaatcc tcgatagccg gtcatgacat
    10801 caagaatttt tgttttatac attctagtaa ttgtatttct aacaaggtta tttccaaaat
    10861 cgtggaaagg tctcttattc tcatcaaagt aggttccatt tgataggcga tcaccaatga
    10921 ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc atgtacagct tctgcaggat
    10981 aagtatcatc gccatctacc attaaataat agtcggcatc gatatcaaag aacatttggc
    11041 ggatcacatt tccttttcct tgacgaggtt ctttttttac gattgctcca ccttgaacgg
    11101 ccaattcgta tgttcgatcg gtcgaattat tatcatagac ataaatatct gcttctggta
    11161 attctcgttt aaagtctgca atgactgttg aaattgttgc ttcctcattg taacaaggaa
    11221 tcagaacagc aattttttct tgttgttcca tttttcactc tccttaacta tagaaaacaa
    11281 aagctaaatt aaaaatacct cataatcaaa ggaaatgcta gtttttcccg ctaaatatca
    11341 tttatttaaa aagaaaatac tggaatatat gtttggaaag ctctatctaa aaaaccaccc
    11401 actacaaaag aaggacatga tacctctctt ttgaatagtg ggtgcgtatt cgttctctca
    11461 cttttttgac gtattaatct gttgcacctt tttttcttga aatttctaat acggttcctg
    11521 tatgagcatc tgctaaaaat tcataataaa cagtttcgcc atcttctatt cgagaaagac
    11581 ctccccgata agcactcgac cgaacggcga atttcctaat aggttccttt tcaaaagaga
    11641 tccaagaacc ttcgattgca ccttctttta gaaaagatga cttgatattt tcaagtactt
    11701 ggtctgctga caatactttt ttgcgatgat accaaatcgt tgaagctacc ccgctcaata
    11761 atcctagacc gattccaact gctaacccgc ctttaaaata gcttagttcg ctttcttcat
    11821 acatgtggac atacctcctt cttctcactt gttactcctt gtgcttattt taccacactc
    11881 tcatattttc tttgaaataa ataaccgcta ccagaaaaaa taagctataa taaatacgat
    11941 cggtctgcag aaaaaatcat attttatccg cagatcaata taagtctgag aggagtttag
    12001 tctgaaattt agacgaatag catatggaag aaaaaacatt tcaacgaatc aaagagttaa
    12061 cagaattaca aggaacaagc ggttttgaac aagatgttcg cgcttatatg gaaagtcata
    12121 tggaaccgct ggtagatgag ttacagttag atggtttggg cggcattttt ggtctgcgcc
    12181 atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc acatatggat gaagtcggat
    12241 tcatgctgac acaaatccaa gaaaacgggc tttttaaagt cgtacctcta ggcggctgga
    12301 atccttacgt tgtttctgct cagcgtttta cacttaaaac ctctactggt aagaattatc
    12361 catgtatttc ttcttccgtt cccccacatt tattgcgagg aacaagcgga caaaaatcag
    12421 tagaagtaac cgatattttg ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt
    12481 acggtgtcct tccaggagac acaatcgttc cttacgctga aacgatcaaa acagccaacg
    12541 gaaagaatat tatcagtaaa tcatgggaca accgctatgg ctgtacaatg gtattagaag
    12601 ctttagaagc gttgcaaaat gaaacattag gtcatacatt gattgcaggc gcgaatgtcc
    12661 aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa taaattcaag ccagatctgt
    12721 tctttgcagt agactgctct gcagcagatg atacagtgac gaaaaatggc acattcggac
    12781 atttaggaga aggtacattg atgcgtatcc aagatccagg tttgatcatg cttcctcgtt
    12841 tgcgcgagta cttgctggat atcgctgaaa caaacaatat cccttatcaa tactttgttt
    12901 caaaaggcgg tacagatgca ggtgctgctc atacacaaaa cgaaggaatc ccaagtacag
    12961 tgattggcgt agtgggacgt tatatccata cgcatcaaac gatgttcagt attcgcgatt
    13021 ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg tttggataaa tcaacagtga
    13081 atacgatcgt atacggaaaa taatgcgata gtgaaactat aaaatctggc aaaagattgc
    13141 tttttgccag atttttttta taaaaagagg atcaatgttt gttagttcga aagaaaggtg
    13201 tataatttaa ttgaaaaata cataaacaga aagtagagga caaagaatga ttatcccaaa
    13261 tagtttagaa gaattagcag gctacgtaga aactggaaag agtgtatttt tctttactgc
    13321 cggctggtgc ggagactgcc gttttatcaa accgcaaatg cctgaaattg aaaatgactt
    13381 ttctgattgg caatttattg aagtagaccg tgacaagtac atagatgtag ccgctgaatg
    13441 gaacattttt ggtatcccaa gttttgtcgt gatccaagac ggaaaagaat taggtcgtct
    13501 agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg ttcctagaaa gtgtaacaaa
    13561 aggctgacat ctgttaggag gggtcaagat gaatcaagaa tataagaata ttttagtagg
    13621 tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa gcggtagaag tggctcgccg
    13681 taataatggg aaagtatatg tagcaaacgt aatcgatcag caattttata actttatggg
    13741 ctatgcacct attgatcaaa atctagtcga tcagcaaact gaaacagcca aggaaatgat
    13801 cgaagaatgt aaagaatatg ggaaatctgt tgattacaca aatatagaag gaattattgc
    13861 atatggttct gttcgtgaat ccatggcagt caaattgcca aaaaaatatg agatcgatct
    13921 aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc tttatgacag gaagtgtagc
    13981 cagctacgtg atacgtcatt caccttgtga tgtcttgatc gtttctgacg aagaagaaca
    14041 gccaagtaat taagcaagtg gaacttgtac gaccttttta atcaattaac taaaacagta
    14101 aagggactgt gacaaaagtc ttgtcacagt cccttgttac gaataaatgg tgttccagaa
    14161 gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc agtgaatgca attttttgtt
    14221 aagataaatc tcagcaaagg ataatggagg aaagaacatg atttttgctt ataacaaagc
    14281 acacgtcgga gacaccttga tggttattat agccgacgat aaaggaacag aaaacactgt
    14341 tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa
    14401 ctttttccat atctccgatc atctaacaat cgaagggaac ggccaagtaa gcttaacaga
    14461 tgaggaatta gcgaaattga atcgattgat caaagaagca ggattcgaag aacagcttga
    14521 agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa acatgcgtcc cacatcctga
    14581 ttctgaccat ttatcgatta ctgagacaga agtagacaat ggtacagtat tgcaaatcgt
    14641 ttgcggtgca cccaatatcg aagctggaca aaaagttgtc gttgcaaaac caggagctat
    14701 gatgccagat ggaatgatga tctggccagg aactttgcga ggggtagata gctatggaat
    14761 gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca gcaaaaaaag gaatcttagt
    14821 attgcctgag gatgccgtag tcggagaagc atttccaaaa taaagaggtt gtgacaagtc
    14881 tttgtcacaa cctctttttt attaattttc actattttga ttttgtttta atgccgattg
    14941 atcgatcgtc aattcaacag tagttgtttt ttctttcgag ccttcatagt aagtgatttt
    15001 gacagaatct cccacttttt tcttatacaa agcagattga agttctacac cagaagaaac
    15061 ttctttgtca tcgatcttag taatgacgtc gtattgtttc aatccagctt tttcagccgg
    15121 tgtagctgtt tggacatttg tcacgatgac accgttagtt acagatgaag gaatcttcaa
    15181 gatttgttct tgttgctgag tagaaacggc agataagtcg accattgtga tacctagagc
    15241 tggacgtgtc acttttccgt ctttttccag ttgattgatg atattcacta catcgttact
    15301 tggaatagca aatcccattc cttctacgct tacattagaa gtagattcag aagtacttgc
    15361 gatcttactt gagttgatac cgataacttg tccttcgata ttaactaaag gaccaccaga
    15421 atttccaggg ttgattgctg catctgtttg gatcgcattg atattgactg tttcgttcga
    15481 ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg attcctgaag tgacagaatt
    15541 tgcatattca gatcctagtg gggaaccaat cgcaatcgca ggctcaccaa ccttcagagc
    15601 acttgagtca ccaaaagagg caactgtttc gactttgtct gaattgatct tcaacaccgc
    15661 taaatctgaa taagcatctg ttccaactaa ttcagctttt accttcgtac cgtctttcat
    15721 caagacttca agaccttgct ggccatctac tacgtgatta ttcgtgacta cataagcagt
    15781 attcccgtct tttttgtaga tcacgccgct tccttcacta taagcttcca aattactatc
    15841 atctgagctg ctactttctt cttgctgccc gaatagtccg ccaaaaccgc ttgattggtt
    15901 ttgactttgt aagttgataa cagaaacgac agcgtcttga actttatcta ccgctttggt
    15961 gatatcagaa tctacattga ctttgatatt ttccacaact gtttcaccgg cagaattctg
    16021 attgccgctt gtagcagtag aactattgtt gccagtgccc atcgctgcat aaaaaatgcc
    16081 agctgtcagt agtcctccaa cgatccctcc aacaagcccc agacctaact tacgccagag
    16141 gctattattt ttactttttt tcatctttgg tgtcacattt tttctatcca tgtgttcctc
    16201 ctccaattac attcatttat ctactgttag tatagtctta tttgttgact ttagtctact
    16261 ttgagactgt gaacttttta tgaaaaactt taggaaaaat cattgttttc agatgaaaat
    16321 atttgatttt ctttttccac agatactgtg taaaactacg cggataggat tggaaaaata
    16381 agggataaaa catatccaca gcctgtgtat aaagtggata aattcttata aaacgtatgt
    16441 ttgcctaatg aatgtgatga aataaggtaa aataacactg ttttctgtgg ataaacctgt
    16501 ggatgttgtg cataactgtt ggggaaatct ttttaacaaa aatagccaaa ataaacatat
    16561 agacgaaaaa attgattata ataaacaaag tgagcatgaa gaccaaagga gagaaaacat
    16621 gaatatcaaa attatctcag taggaaaatt aaaagaaaaa tacctgatcc aaggtatcaa
    16681 tgagtatgtc aaacggctga atgcctatgc aaaaatcgaa ttgatcgaag ttcctgacga
    16741 aaaagccccg gagaatttaa gcgaagcgca aatgagacaa gtaaaagaaa aagaagggga
    16801 acggatctta gcgaaaatca aagaacaaga atacgtctat gccttagcaa ttgaagggaa
    16861 aaatcctaca agtgaatctt ttgctaaaca aatcgaccaa ttaggtatcc aaggaaaaag
    16921 ccaccttgtt tttgttatcg gcggctcttt aggactcagc gaagccgtca tgaaaagaag
    16981 caatgctcag atctcttttg gaaaaatgac gtatccacat caattgatgc gtctgatcct
    17041 tgtcgagcaa atctaccgtg ccttccggat caatgcgggg gcgccttatc ataagtgatg
    17101 cggtttttat cattgatcct aaaaatctta gcaagacaaa aagtacgtaa gaaagtaaag
    17161 actaaatact tcggaggaac aaatgaataa caatgatagg ttattgcgat tgcgttatgc
    17221 gattgatttg aaagatacgg atttgattaa agcttttgag ctaggcggtg taccactgac
    17281 taaagaagaa gctcaggcgg tactgaccaa agtacaggat aagaacaaag ataatgcaga
    17341 aaataatgtg tatgaaaaaa ccatcaacaa ccaagttttt gacgcattcc taaatggcct
    17401 gattctcttg gcgcgtggtc cgcaaaaaga taagccgata gtagacacgc cgcaaaaaag
    17461 caaggaaatc aaatatatca acaatgtgtt gctaaaaaaa ttaaaaatcg ccttgtctat
    17521 gaccactgat gatattttag acgtttttgc tgaggcagaa atctatccat caaaaggcga
    17581 aatcggggca tttttgagaa aagaaggcca acgaaatttt aagccttgcg gagataaata
    17641 tatgcgtaat tttctcaagg ggttaggaat ttataatcgg agaaaagtat aaagaaattt
    17701 catgagaaat caaaacaatg gttgttttga tgagagctag tttttgtatg agattaaaat
    17761 attatcttaa tcgaggagct ggattggttt tatgaatgat gaatataaga atgatgaaga
    17821 taagatgctt tttgaagaga tagagaatcg atgtaggctc aattttgagc ttcggggaaa
    17881 gatgtcttta atacaacaaa aaaagtattt agcgaataaa tcagagttta ctctgggaca
    17941 tgttgaaaaa ttaatctcgg attggattag tagtagaagc gaatttacaa aaataaaaca
    18001 gccgattaaa tttgacatga aaaaactttt gttaaataag agtgagatag gtaatagaga
    18061 tcaatatatt agagctaaag ggcaagaaat aattgattca ttaggtgaaa tgaggtcata
    18121 caattatttg tatgtaacac atagagctga tggaatggtc attactgtgg ggaaatcttc
    18181 ttctaatgat atttttttag atggagatct tttttatcaa ctcaatacaa atcacttgtc
    18241 tggcactgaa aatataattt taaggacgga atatggcaat gaaatatttg caaaatatga
    18301 tgagatttta aaaaattatt tggactgggc ttggataatt ccggttgagt caggagatgc
    18361 taagaaactg gagagattat tgggcgatga actaattaat aaaaaagtac caattttaaa
    18421 ttattattcc catagacaat aaaaggctat ttttcatgat aatactagtt ataactgtaa
    18481 aaatggaaga aaatctggga aagaagtttt taaataagtg tattgttcga ttttaataaa
    18541 taataagcaa taccatccgt ctgtgttgcg caacatcgta ctacaatgaa tattttttca
    18601 caaaaagaaa atcatccatc gaatgaatgg aaggttttag tatacacgag gttaat
//
LOCUS       DAFBQW010000054        17107 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFBQW010000054 DAFBQW010000000
VERSION     DAFBQW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.056"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            7..1398
                     /locus_tag="LK889_002120"
     CDS             7..1398
                     /locus_tag="LK889_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK5096330.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="LK889_002121"
     CDS             1414..2454
                     /locus_tag="LK889_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5096331.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="LK889_002122"
     CDS             2473..3558
                     /locus_tag="LK889_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBK5096332.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="LK889_002123"
     CDS             3591..4553
                     /locus_tag="LK889_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK5096333.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="LK889_002124"
     CDS             4546..5979
                     /locus_tag="LK889_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK5096334.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="LK889_002125"
     CDS             5976..7046
                     /locus_tag="LK889_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096335.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="LK889_002126"
     CDS             7033..8454
                     /locus_tag="LK889_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK5096336.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="LK889_002127"
     CDS             8467..9474
                     /locus_tag="LK889_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5096337.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="LK889_002128"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="LK889_002128"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBK5096338.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="LK889_002129"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="LK889_002129"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBK5096339.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="LK889_002130"
     CDS             11608..12285
                     /locus_tag="LK889_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK5096340.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="LK889_002131"
     CDS             12275..13417
                     /locus_tag="LK889_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK5096341.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="LK889_002132"
     CDS             13430..14407
                     /locus_tag="LK889_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5096342.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="LK889_002133"
     CDS             14407..15315
                     /locus_tag="LK889_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBK5096343.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="LK889_002134"
     CDS             15316..16068
                     /locus_tag="LK889_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBK5096344.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="LK889_002135"
     CDS             16073..17020
                     /locus_tag="LK889_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK5096345.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6164 a         2190 c         3090 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaa
//
LOCUS       DAFBQW010000055        17004 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFBQW010000055 DAFBQW010000000
VERSION     DAFBQW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.057"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..124)
                     /locus_tag="LK889_002136"
     CDS             complement(<1..124)
                     /locus_tag="LK889_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBK5096346.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="LK889_002137"
     CDS             complement(228..539)
                     /locus_tag="LK889_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK5096347.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="LK889_002138"
     CDS             complement(541..738)
                     /locus_tag="LK889_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBK5096348.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="LK889_002139"
     CDS             complement(1132..2859)
                     /locus_tag="LK889_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBK5096349.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="LK889_002140"
                     /pseudo
     CDS             complement(2852..4045)
                     /locus_tag="LK889_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
     gene            complement(4232..4876)
                     /locus_tag="LK889_002141"
     CDS             complement(4232..4876)
                     /locus_tag="LK889_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5096350.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="LK889_002142"
     CDS             complement(4970..5104)
                     /locus_tag="LK889_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBK5096351.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="LK889_002143"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="LK889_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBK5096352.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="LK889_002144"
     CDS             complement(7235..7708)
                     /locus_tag="LK889_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBK5096353.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="LK889_002145"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="LK889_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK5096354.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="LK889_002146"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="LK889_002146"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK5096355.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="LK889_002147"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="LK889_002147"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK5096356.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="LK889_002148"
     CDS             complement(11755..12894)
                     /locus_tag="LK889_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5096357.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="LK889_002149"
     CDS             complement(13203..13916)
                     /locus_tag="LK889_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK5096358.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="LK889_002150"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="LK889_002150"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK5096359.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15104..16636
                     /locus_tag="LK889_002151"
     CDS             15104..16636
                     /locus_tag="LK889_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5096360.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16827..>17004)
                     /locus_tag="LK889_002152"
     CDS             complement(16827..>17004)
                     /locus_tag="LK889_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5096361.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5322 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc tttttatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgaa aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaacc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    15001 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    15061 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    15121 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    15181 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    15241 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    15301 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    15361 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    15421 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    15481 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    15541 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    15601 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    15661 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    15721 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    15781 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    15841 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    15901 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    15961 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    16021 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    16081 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    16141 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
    16201 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    16261 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    16321 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    16381 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    16441 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    16501 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    16561 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    16621 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    16681 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    16741 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    16801 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    16861 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    16921 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    16981 ataaagtaca taaacataaa taca
//
LOCUS       DAFBQW010000056        16700 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFBQW010000056 DAFBQW010000000
VERSION     DAFBQW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.059"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            176..403
                     /locus_tag="LK889_002153"
     CDS             176..403
                     /locus_tag="LK889_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK5096362.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            419..1585
                     /locus_tag="LK889_002154"
     CDS             419..1585
                     /locus_tag="LK889_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK5096363.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1820..2863
                     /gene="hrcA"
                     /locus_tag="LK889_002155"
     CDS             1820..2863
                     /gene="hrcA"
                     /locus_tag="LK889_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBK5096364.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2957..3520
                     /gene="grpE"
                     /locus_tag="LK889_002156"
     CDS             2957..3520
                     /gene="grpE"
                     /locus_tag="LK889_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK5096365.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3571..5400
                     /gene="dnaK"
                     /locus_tag="LK889_002157"
     CDS             3571..5400
                     /gene="dnaK"
                     /locus_tag="LK889_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK5096366.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGKRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5551..6717
                     /gene="dnaJ"
                     /locus_tag="LK889_002158"
     CDS             5551..6717
                     /gene="dnaJ"
                     /locus_tag="LK889_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK5096367.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7085..7822)
                     /locus_tag="LK889_002159"
     CDS             complement(7085..7822)
                     /locus_tag="LK889_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5096368.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7824..9326)
                     /locus_tag="LK889_002160"
     CDS             complement(7824..9326)
                     /locus_tag="LK889_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBK5096369.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9585..11579
                     /gene="uvrB"
                     /locus_tag="LK889_002161"
     CDS             9585..11579
                     /gene="uvrB"
                     /locus_tag="LK889_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBK5096370.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11589..14405
                     /gene="uvrA"
                     /locus_tag="LK889_002162"
     CDS             11589..14405
                     /gene="uvrA"
                     /locus_tag="LK889_002162"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK5096371.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14668..15933
                     /locus_tag="LK889_002163"
     CDS             14668..15933
                     /locus_tag="LK889_002163"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBK5096372.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYAGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15937..16668
                     /locus_tag="LK889_002164"
     CDS             15937..16668
                     /locus_tag="LK889_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBK5096373.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
BASE COUNT         5504 a         2901 c         3661 g         4634 t
ORIGIN      
        1 tttttatgaa taaaataagg acaaaattgt ttaggaagaa tcatgagtgt aaaaaaatca
       61 tccaagaagt cgattcttgg attttttagt ggatttgttc gccaaatacg tttagtatgc
      121 gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa
      181 aatagctttt catatttttg cagattttga cttaatgccg aaggtcagct ttgcgattac
      241 cgtttatcga gaggctgtaa aagcaccgtg tagctccgat gacaataagc aagcattttt
      301 agagctaggt ttccttgatt ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg
      361 cgattttaag aaggtcgaaa acaggcacga cttttcgacg taaggagaaa gaatttcaat
      421 gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg tacattcata tcccattttg
      481 cgagcatatc tgctattact gtgactttaa taaagttttt ttagaagggc agccagtaga
      541 tgagtatgtg gaaatgctgc tgaatgaaat gagattaatg ctggaaagtc atcctacagc
      601 gaatttggaa acactttatg taggaggcgg aacaccaact tctttatctg ctaaacaact
      661 tgatcgacta ttatcaggtg ctagagagat cctgcctttt caaaatggta atgagtttac
      721 tgtagaagcg aatcctggtg atttgacccg tgaaaaactt caagtgatga aaaactatgg
      781 cgtcaatcgt ttatccatgg gagtgcagac atttgacaat cgcttattga aaaaaattgg
      841 acgtaaacat actgcagaag atgtgtatca aacaatgcaa tttttagaag cagaaaattt
      901 taccaatgta agcatcgatt tgatttatgc attaccggga caaacactag aaggatatcg
      961 ggatactttg gaaaaagctt tggctttgga tctgcctcac tattctttgt actcattgat
     1021 cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg aactgcctga
     1081 tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa agcatggacg
     1141 tcaccaatat gaaataagta actttgggct ctccggacat gaatctcaac ataacctcat
     1201 gtactggaac aatgaccact attttgggtt tggagctggt gctagcggtt atcttggaga
     1261 tattcgttac cgcaacaaag gaccgatcca acactattta cgcccattac gagcaaatga
     1321 acgcccggta ttggaagaag aaaaattgac acgaaaaaat caaatagaag aagaaatgtt
     1381 cttaggacta agaaaaaaaa cagggattac taaaaaacat ttctatgatc gctatcaaaa
     1441 aacaattgaa tcggtgtacg gtgatgtgct aagggaatta gagcaagaag ggttacttgt
     1501 taatgaagaa gaccgtgttt atttgacacc aagagggact tttttgggga acaatgtgtt
     1561 tgagcgcttt ttatttgaaa aatagtttga agaatatttg ttttttatcg aaacttgaaa
     1621 agaaaaagtt tagaaagttt gacaatgagg tattgaagta ttgatataac tggatttaca
     1681 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
     1741 gacaggagaa gggcttcttg ctatatttat aagtgaatta gcacttagaa tatatgagtg
     1801 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
     1861 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattaatgt ctgagggagt
     1921 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
     1981 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
     2041 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
     2101 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
     2161 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
     2221 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
     2281 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
     2341 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
     2401 taaacttcgt acggagattc cgatgattct tcataaatat ttccagacga cagaagggat
     2461 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gaaaaggttt atgtcagtgg
     2521 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
     2581 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
     2641 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     2701 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     2761 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     2821 acttgctgat tattaccgct cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     2881 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     2941 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     3001 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     3061 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagatagata
     3121 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     3181 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     3241 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     3301 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     3361 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     3421 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     3481 tcgcgtgtta agaccaacta tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     3541 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     3601 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     3661 gaaggaaaac gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     3721 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     3781 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     3841 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     3901 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     3961 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     4021 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     4081 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     4141 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     4201 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     4261 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     4321 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     4381 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     4441 gtacgtcaag cgttaaaaga tgcaggtctg tcacaatcag aaatcgacga agttatttta
     4501 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     4561 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     4621 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     4681 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     4741 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     4801 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     4861 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     4921 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     4981 acgatcaaat cttcttcagg tctaacagat gaagaaatcg aacgtatggt gaaagatgca
     5041 gaagcaaacg ctgaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
     5101 gacgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
     5161 gaagaagtga aaaaagcaga aacagctcgt gacgaattaa aagccgctgt tgaagcaaac
     5221 aacatcgagg aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
     5281 gttaaactat acgaacaagc tgcacaacaa caagctcaag aaaatccaga agcagctcaa
     5341 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
     5401 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
     5461 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
     5521 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
     5581 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
     5641 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
     5701 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatacga ccaatacggg
     5761 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
     5821 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
     5881 ggaggcggtc gatcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
     5941 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
     6001 gaagaaatct gtcatacttg tggtggaaac ggtgcaaaac caggcacaca accaactact
     6061 tgtcataaat gtcatggtgc aggtacgatc aatgtagaac gccaaacgcc acttggacga
     6121 gtgatgagcc gtcaaacttg tgatgtttgt catggcacag ggaaagaaat caaagaacct
     6181 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
     6241 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
     6301 aacggcggac catttggtga tttgtatgtg gtctttcgtg tagaagaaag tgatatcttt
     6361 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
     6421 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
     6481 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
     6541 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
     6601 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
     6661 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
     6721 ttctagtaaa aatataaagt agacttgccc aaattttcta tttctagtaa aaacgctaag
     6781 tcattaataa ttaaaaacca gacgaacaat catacgttcg cctggttttt ttgtagttgt
     6841 catgtttgta ccaacaaaaa agccagcttt agaataagat aatcatcata gtttctgaag
     6901 gtggtatcga ctatgtactt gatatcgtca atgaaccgat ggtactttct tatgcgatgg
     6961 aacaggtaga gcgattgctt gtttgtgcag gagaacaagc gatgcggctg gtacagttaa
     7021 gtgaaaaaac aagaaactaa aaatgcccgg tcgtcatgaa gcaaccgggc atttttggtt
     7081 tttattaaat attcaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt
     7141 caaagatttg ttgaggcgtt ccgtcttcta aaaagtttcc gtcgtcaatg aacatgacac
     7201 gattggccac ttctttagcg aagcccattt cgtgcgtaac gataaccatc gtcatacctt
     7261 gttctgctaa ctttttcatg acgttcaaga catcaccgac catttcgggg tcgagtgcag
     7321 atgtaggctc atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca
     7381 cacgctgttt ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac
     7441 caactgtctg cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca
     7501 ggtccatcgg tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt
     7561 gctggaatac cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca
     7621 agtgggcgcc gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac
     7681 gcaaaaatgt actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt
     7741 tgatggatac attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct
     7801 caactaagat tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt
     7861 gtcaatgctg tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt
     7921 tgtgttgtac gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg
     7981 gaaatgatcg ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg
     8041 atgcgtattg cttgtggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa
     8101 cttctggaag cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct
     8161 gcgatataag cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc
     8221 gtaaatccaa cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga
     8281 attcctcgga tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct
     8341 acactgaata atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt
     8401 aggagaattg ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca
     8461 agaaatgttg attcatcagc agtggaagac tcactagaag tttctgaacc atccgctaca
     8521 tattctttga taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg
     8581 aacatattaa gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca
     8641 gattcttttt cgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc
     8701 ggataatcat ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca
     8761 gcagcatcaa aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca
     8821 gcactttcgg taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg
     8881 atcgaagtat ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa
     8941 aaatcatagc ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct
     9001 tgtcctgatt ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta
     9061 aatccttgca gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc
     9121 tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct
     9181 tttttgggct ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg
     9241 tcagcttgtg caacatgacc aaacgcaagt gtcacaagtc ctgtcattaa gaaaacaaat
     9301 gagtgaaata aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt
     9361 ataggcgaaa agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga
     9421 gtttctaaca ttttatgtta taaaacgtaa catgtataat aagataaaaa agtggcgaaa
     9481 gtatgttcgg tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta
     9541 ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata
     9601 cctcccgaca ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga
     9661 ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga
     9721 ctggaacagg gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac
     9781 tgattattgc tcataataaa acattggctg gtcagctgta cggggaattc aaggaatttt
     9841 tccctaacaa tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt
     9901 atgtgccttc aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca
     9961 aattacgaca ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat
    10021 ccgtttcatg tatttttgga ttaggttcgc cttttgagta ccaaaaacaa gtagtttcga
    10081 ttcgacaggg agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat
    10141 tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg
    10201 agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg
    10261 tcgaacgtat ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg
    10321 tggcaatctt cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag
    10381 cgaatatcaa agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc
    10441 tggaagctca acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg
    10501 gctacacgtc gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac
    10561 cgccttatac gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc
    10621 acgtaaccat gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc
    10681 tagtagatta cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag
    10741 agtttgaaaa acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac
    10801 atgaacaaac agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag
    10861 tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac
    10921 gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc
    10981 tcacagatta tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa
    11041 cgctggaacg aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg
    11101 gaatcaattt gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg
    11161 atgcggataa agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag
    11221 ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc
    11281 gtctagcgat ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac
    11341 atgggatcgt tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag
    11401 aatcggaaga tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag
    11461 aaaaagacac gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg
    11521 attttgaaac agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg
    11581 aggacaacat ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa
    11641 atatagatgt aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg
    11701 gaaaaagttc cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga
    11761 gcctttctgc ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca
    11821 tcgacggatt gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt
    11881 cgacagtagg tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag
    11941 gacatccgat ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg
    12001 cagatcaagt aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg
    12061 taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc
    12121 gcgttcgagt cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca
    12181 aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc
    12241 gcctatttga ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg
    12301 tgatcggtga agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta
    12361 ctgttggcga actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag
    12421 actgtgatgg tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa
    12481 cgaagacact tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc
    12541 ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag
    12601 aattaccgga agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc
    12661 attatgaaaa tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga
    12721 agaatatcga gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc
    12781 tttatatgac agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc
    12841 tatctgttca aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag
    12901 cagtagattt cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga
    12961 ttttaaaaga agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa
    13021 ctttgagtcg cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta
    13081 cacaaattgg atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat
    13141 tgcatcaacg cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca
    13201 acaccctcgt tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg
    13261 acgtcggacc tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag
    13321 aagtagctaa tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc
    13381 ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag
    13441 aaaacaatct gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag
    13501 gtgtgtctgg ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc
    13561 aaaaattaaa taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa
    13621 gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc
    13681 cagcgactta tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg
    13741 caaaaatgcg gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg
    13801 aagcttgtcg cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg
    13861 ttccttgtga agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca
    13921 aaggaaaaag cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca
    13981 agcacattcc aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg
    14041 tgacgatggg gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg
    14101 ccagtgaatt gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga
    14161 caggacttca tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg
    14221 ctggtaacac agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata
    14281 tcattgattt aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac
    14341 cagaagaaat cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg
    14401 tataaagagg atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg
    14461 ttttattaat atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta
    14521 tagataaaaa cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact
    14581 ttcctttgat tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa
    14641 caagagatta tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc
    14701 cctatattat taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca
    14761 tacggcaaaa tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa
    14821 attgtcaatg tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg
    14881 cttcgtttgg caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt
    14941 ggtgatggat atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc
    15001 tgtccatata ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa
    15061 gtttgtgaag aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta
    15121 gtaaatggac atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca
    15181 aacagtgtgg agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag
    15241 actagagaag agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa
    15301 atgattgtac aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat
    15361 gtagatgaag atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct
    15421 acaccagcat cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat
    15481 caaaccatta aggcattttt ggaaaaaatc gaatatgcag gctttgccaa tttcgatatg
    15541 aagtatgatc ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt
    15601 agcagtttct ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga
    15661 gtgttcaaca aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga
    15721 ttatggatgg gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa
    15781 aaactagctg agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa
    15841 gaccgatcaa tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga
    15901 ttcaaaaagt attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta
    15961 ggcggaatgg gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa
    16021 gctacaaaag atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat
    16081 cgtacagctt atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat
    16141 attgagaaac aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac
    16201 tacttttttg aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa
    16261 gcagcaaatg aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa
    16321 ggaagtatga aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg
    16381 attcctgata ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa
    16441 tcggattatt tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta
    16501 aactgtgaaa aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa
    16561 gataatcatt acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt
    16621 gcattagctg aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt
    16681 tggggcaaat atcctaaaag
//
LOCUS       DAFBQW010000057        16669 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFBQW010000057 DAFBQW010000000
VERSION     DAFBQW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.060"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1350
                     /locus_tag="LK889_002165"
     CDS             <1..1350
                     /locus_tag="LK889_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5096374.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="LK889_002166"
     CDS             1484..2827
                     /locus_tag="LK889_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5096375.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="LK889_002167"
     CDS             2939..4390
                     /locus_tag="LK889_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBK5096376.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="LK889_002168"
     CDS             4739..5371
                     /locus_tag="LK889_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBK5096377.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5959..7398
                     /locus_tag="LK889_002169"
     CDS             5959..7398
                     /locus_tag="LK889_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5096378.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7693..7911
                     /locus_tag="LK889_002170"
                     /pseudo
     CDS             7693..7911
                     /locus_tag="LK889_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8160..8375
                     /locus_tag="LK889_002171"
     CDS             8160..8375
                     /locus_tag="LK889_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096379.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8592..8848)
                     /locus_tag="LK889_002172"
                     /pseudo
     CDS             complement(8592..8848)
                     /locus_tag="LK889_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9035..9946)
                     /locus_tag="LK889_002173"
     CDS             complement(9035..9946)
                     /locus_tag="LK889_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5096380.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9965..10969)
                     /locus_tag="LK889_002174"
     CDS             complement(9965..10969)
                     /locus_tag="LK889_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK5096381.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10966..13089)
                     /locus_tag="LK889_002175"
     CDS             complement(10966..13089)
                     /locus_tag="LK889_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5096382.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13094..15541)
                     /locus_tag="LK889_002176"
     CDS             complement(13094..15541)
                     /locus_tag="LK889_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5096383.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15528..15917)
                     /locus_tag="LK889_002177"
     CDS             complement(15528..15917)
                     /locus_tag="LK889_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5096384.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15977..16480)
                     /locus_tag="LK889_002178"
     CDS             complement(15977..16480)
                     /locus_tag="LK889_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK5096385.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16493..16654)
                     /locus_tag="LK889_002179"
     CDS             complement(16493..16654)
                     /locus_tag="LK889_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096386.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         5384 a         3107 c         3072 g         5106 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     5581 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     5641 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     5701 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     5761 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     5821 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     5881 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     5941 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     6001 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     6061 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     6121 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     6181 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     6241 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     6301 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     6361 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     6421 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     6481 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     6541 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     6601 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     6661 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     6721 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     6781 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     6841 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     6901 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     6961 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     7021 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     7081 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     7141 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     7201 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     7261 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     7321 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     7381 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     7441 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     7501 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     7561 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     7621 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     7681 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     7741 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     7801 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     7861 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     7921 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     7981 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     8041 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     8101 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     8161 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     8221 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     8281 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     8341 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     8401 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     8461 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     8521 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     8581 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     8641 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     8701 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     8761 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     8821 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     8881 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     8941 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     9001 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     9061 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     9121 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     9181 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     9241 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     9301 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     9361 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     9421 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     9481 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     9541 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     9601 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     9661 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     9721 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     9781 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     9841 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     9901 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     9961 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
    10021 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
    10081 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
    10141 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
    10201 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
    10261 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
    10321 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
    10381 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
    10441 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
    10501 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
    10561 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
    10621 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
    10681 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
    10741 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
    10801 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
    10861 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
    10921 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
    10981 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
    11041 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
    11101 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
    11161 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
    11221 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
    11281 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
    11341 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
    11401 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
    11461 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
    11521 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
    11581 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
    11641 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
    11701 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
    11761 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
    11821 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
    11881 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
    11941 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
    12001 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
    12061 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
    12121 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
    12181 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
    12241 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
    12301 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
    12361 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
    12421 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
    12481 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
    12541 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
    12601 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
    12661 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
    12721 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
    12781 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
    12841 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
    12901 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    12961 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    13021 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    13081 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    13141 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    13201 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    13261 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    13321 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    13381 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    13441 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    13501 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    13561 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    13621 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    13681 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    13741 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    13801 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    13861 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    13921 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    13981 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca ccccgttccc
    14041 cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa actaagagat
    14101 tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct agcgcattgg
    14161 taactgaacc cttgacccct tgtgaggcca atgccggttt caggtaaaca ttgcgccccg
    14221 tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga gtggcgccaa
    14281 aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc atgtatcgct
    14341 tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc cggactagtt
    14401 ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt agttcatcta
    14461 ctgattcggc acttactcga accacatagc tcagtttgta catggattct ttggactgat
    14521 ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg gaatttgttt
    14581 cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc ttattgcgaa
    14641 cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg tcaattggga
    14701 atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc atttccccca
    14761 caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt tccatgcata
    14821 agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg taacgtttca
    14881 ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct tcaaatggtg
    14941 tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt gtgactcgtc
    15001 tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg gtatatcggc
    15061 gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga
    15121 caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg acctcttcat
    15181 ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta tcgccaatca
    15241 tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct gttaatcgcc
    15301 cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct gtggcaattt
    15361 gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag ttatcgtgga
    15421 cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac tcatagtagg
    15481 caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta atcgggtaca
    15541 tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg ttctttgact
    15601 ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat
    15661 cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac tgccaccggt
    15721 atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac atgggcaaat
    15781 acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt cacaggaaaa
    15841 ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat actggtgtag
    15901 ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag aaatcaacta
    15961 cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt accaaataat
    16021 tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    16081 ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca tcactcatat
    16141 catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc tcaaaagaat
    16201 tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct tcaagttcct
    16261 ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca atctcaaagg
    16321 gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaatcca atccgttctt
    16381 tcacttcatc aaaatcgacg ggtggggtaa accatgcccc caccaactca ccttcattgt
    16441 attttccaag gttcgcaata taaacacgca tctgttccat cgtgcttccc cctcaggcac
    16501 cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca ttaaagacca
    16561 atccgaccgc aatcaaagca atcaccaaga agccaatcag tttggaaaac tcgcgtttaa
    16621 atcctaaaaa caaaccaatg accacaatcg ccagtaagac taaggattg
//
LOCUS       DAFBQW010000058        16523 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFBQW010000058 DAFBQW010000000
VERSION     DAFBQW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.061"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(141..416)
                     /locus_tag="LK889_002180"
     CDS             complement(141..416)
                     /locus_tag="LK889_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBK5096387.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /gene="der"
                     /locus_tag="LK889_002181"
     CDS             complement(659..1969)
                     /gene="der"
                     /locus_tag="LK889_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK5096388.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LK889_002182"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LK889_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK5096389.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="LK889_002183"
     CDS             complement(3515..4195)
                     /locus_tag="LK889_002183"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK5096390.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="LK889_002184"
     CDS             complement(4244..4879)
                     /locus_tag="LK889_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5096391.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="LK889_002185"
     CDS             complement(4945..6348)
                     /locus_tag="LK889_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK5096392.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="LK889_002186"
     CDS             complement(6335..7366)
                     /locus_tag="LK889_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096393.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNGFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="LK889_002187"
     CDS             7397..7618
                     /locus_tag="LK889_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBK5096394.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="LK889_002188"
     CDS             complement(7783..8520)
                     /locus_tag="LK889_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBK5096395.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="LK889_002189"
     CDS             complement(8673..10013)
                     /locus_tag="LK889_002189"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBK5096396.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="LK889_002190"
     CDS             complement(10250..11773)
                     /locus_tag="LK889_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK5096397.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="LK889_002191"
     CDS             complement(12151..12762)
                     /locus_tag="LK889_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5096398.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="LK889_002192"
     CDS             complement(13092..13808)
                     /locus_tag="LK889_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK5096399.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LK889_002193"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LK889_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBK5096400.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="LK889_002194"
     CDS             complement(14367..15164)
                     /locus_tag="LK889_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBK5096401.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="LK889_002195"
     CDS             complement(15134..15505)
                     /locus_tag="LK889_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK5096402.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LK889_002196"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LK889_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK5096403.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5503 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaaccgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacttgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttc
//
LOCUS       DAFBQW010000059        16437 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFBQW010000059 DAFBQW010000000
VERSION     DAFBQW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.062"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(22..1200)
                     /locus_tag="LK889_002197"
                     /pseudo
     CDS             complement(22..1200)
                     /locus_tag="LK889_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1230..1640
                     /locus_tag="LK889_002198"
     CDS             1230..1640
                     /locus_tag="LK889_002198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096404.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            1721..2341
                     /locus_tag="LK889_002199"
     CDS             1721..2341
                     /locus_tag="LK889_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096405.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3042..3326
                     /locus_tag="LK889_002200"
     CDS             3042..3326
                     /locus_tag="LK889_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096406.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            3329..4246
                     /locus_tag="LK889_002201"
     CDS             3329..4246
                     /locus_tag="LK889_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5096407.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4239..5021
                     /locus_tag="LK889_002202"
     CDS             4239..5021
                     /locus_tag="LK889_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096408.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5022..5702
                     /locus_tag="LK889_002203"
     CDS             5022..5702
                     /locus_tag="LK889_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5096409.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            5695..6591
                     /locus_tag="LK889_002204"
     CDS             5695..6591
                     /locus_tag="LK889_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5096410.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            6786..8435
                     /locus_tag="LK889_002205"
     CDS             6786..8435
                     /locus_tag="LK889_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK5096411.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9029..9964
                     /locus_tag="LK889_002206"
     CDS             9029..9964
                     /locus_tag="LK889_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096412.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            9957..10727
                     /locus_tag="LK889_002207"
     CDS             9957..10727
                     /locus_tag="LK889_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096413.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            11143..12126
                     /locus_tag="LK889_002208"
     CDS             11143..12126
                     /locus_tag="LK889_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBK5096414.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            12573..13607
                     /locus_tag="LK889_002209"
     CDS             12573..13607
                     /locus_tag="LK889_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5096415.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
     gene            complement(13696..14019)
                     /locus_tag="LK889_002210"
     CDS             complement(13696..14019)
                     /locus_tag="LK889_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK5096416.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(14006..14713)
                     /locus_tag="LK889_002211"
     CDS             complement(14006..14713)
                     /locus_tag="LK889_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK5096417.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            14799..15140
                     /locus_tag="LK889_002212"
     CDS             14799..15140
                     /locus_tag="LK889_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBK5096418.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(15311..16159)
                     /locus_tag="LK889_002213"
     CDS             complement(15311..16159)
                     /locus_tag="LK889_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096419.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         5203 a         2831 c         3454 g         4949 t
ORIGIN      
        1 gtccttctgc aaaataatgg gttactcagt aaacattgaa gctaatgtat ccgtcacttg
       61 ttggaagcct ttatggcttc tatttaagaa tttttgattg tatgtatcaa agatggatac
      121 taggaaacgc tctagtgatt cttcattttg aaactgctct tttctgcggc tgtatttctt
      181 gatttgtttc ttgaaagatt cgattaaatt agttgagtaa atgcttcggc ggataccagg
      241 cggaaactca taaaaagtca ataaatcttg attttttatc agtgactgcg tcactttagg
      301 ataagttttt tgctacttct caatcatact ccctaaaaag gtatttgcct cttcctttga
      361 gctagcttga taaacagcct taaaatcatc acagatttct tttcgatctt tgacacgtac
      421 tttatgagca atattacggg aaacgtgaac acagcaatgt tggtatttag ctttaggata
      481 aatctggtga atcgtatctt tcatgccttt taagccatcg gtaataaaca gtaagacatc
      541 atgaacccct ctggagtaaa tgtcctgtaa caactcattc caaacgtagg tcgattcagt
      601 cggagcaatc gcataactta gtacttcttt agtgccgtct tctcgtatac caatggcaat
      661 atagattgct tctttggaga cggtttgccg ttttaaagga atataagtag catccatgaa
      721 aatagcgaca tacttatcat ttagagttct aactttaaat gcatttactt cttcagtcag
      781 cactttagtc atgttggaca tggtttgggg cgtgtagtga tgcccataca ttttctcaat
      841 caagtctgcg atttccgaca tggtaatacc ttttcggaag aggtgaatga cggtctcctc
      901 taaggtatca ttcgtccgtc tataagcagg aacagtctgt tgcttgaact cgccgttgcg
      961 gtcgcgcgga atctggagat gaagttcccc gtactcggtc ttgaccgtac ggtcatagga
     1021 gccgttacga gaattccccg tgttaaaacc aatgcgatcg tacttttcgt aatcgaggaa
     1081 agccgttaac tccgttttga gaagcgtatt gacagctttt tccaaatgga cacggaataa
     1141 ttcattcaaa tcgcctttgt tcgctagagt ctttagaatt tctgtagtaa aatcgttcat
     1201 atggaagtcc tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc
     1261 ttttttctat ttacacaaaa tattttacac tctcgtcaaa tataaaaact agtacgaaat
     1321 taatttgtaa attatttaag cttgataaaa taagcaatat cgatgatgta aagtccaaga
     1381 tattaacaag aaaagtaagc gaaccagtaa agctaacggc acaagataaa aaaaatggaa
     1441 ttgttcttta tggtgatgat gcaaagtata actataattg ggatacctcc tcagaagctg
     1501 aggtattaag tattcctact accgatactt caggggaaaa agtcaccttt aaaataaatt
     1561 ttataacggc aatttctaag ttggagtatg ggaagcaaaa aaagagagat aacaatgatc
     1621 aatatctacc taaaaaataa cgaattaatg atgcatatgt taaagttgtt tttttgaatt
     1681 aaacaactca atttattcag ttatatgttc atcgcagcaa gtgaacatta atcgagttat
     1741 aaaattaata ggagctaaaa atataaataa agttcgatca gaatttattt ttccagaaga
     1801 tttttttaac tggttttttt atcaattcag tgaatgcaag cgactcttag gtgagaatta
     1861 ttacttaaaa ggaatcggcg gatttatggg gaatgtggct gatgaacaaa atattataaa
     1921 aggaatatct gaacaaacat cagagcttac agtaactaaa gcatttataa gtaatggtga
     1981 aagtttgaaa aatgtaactg cgcgaattag aaaagaaaat gaagtagaca ttgtttttgg
     2041 aattgaccat gaatgtaatt cagtgatata tgtaacacaa tcagcaaatt taattctttt
     2101 tgagaatggt tgcaaggacg attttatggt ggtctatttg tacgatatac taattccaaa
     2161 acttcgttcc ttatatatgt cttctgaaag tatttttgtg agtgaacaaa agggatcttt
     2221 ctcgaaaaaa ataggtattg aagtaattga atcgattatg tgtcataatc atattacgct
     2281 agaagaatta gaaattttga tagggcaaaa aaataataac acgaaaatac aagtttctta
     2341 gaaatcattt tttattgaga tatctagata aatgatttat tcttcaatca accaactcgc
     2401 caagctgagg taatgttttg aggtaatgat aacatacaac tttttatacc ctagtaaaac
     2461 tctataaaca aagacttatt aatttcgcat gaaatacatt cctgtaaaat gcaataaaat
     2521 agagttttgt ttaaccaggt cttaagaaag cccgtaaagc ttcacagttc tcaaaacgtt
     2581 aattgcgatt atcgttcgag ttcacgagtt acgagaatcg tactgggcga agcaatgctg
     2641 agcgcagtta ccttaatcct aacttttctg gcactttcca caatttggaa agtgcctttt
     2701 ttgtgttttt agctatgtta ataggtagga ttcttggtct ataatatcat tcgatctata
     2761 aacaaacggg aaaaagataa agtatcatca aacaatatta tttatccgct tctgaggttg
     2821 tgacagaagt ggtcagcttc aagaaataag aaggaattta caaaaattgc ttctcatgtt
     2881 tgataaatat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt attcggaaga
     2941 agggattgtg aaaagtcttt tgtcataacc tcttatttgg tagctaattg taaatcccaa
     3001 acggcatgta ttatttttca taagtccgtt tttaaggatt aatgttttct tttgcttttt
     3061 atatggtctg gcttcagcga gaagttccaa cgcatataca aggacgtctg atgtcaacga
     3121 taacaacgat taccatggtg gccagtccac ttggattagc actgtttggt gcgctctttg
     3181 attggcagac aactcaacca cgtctgcgag acagtctgat ttttcttgaa attcaattgg
     3241 aaaatttaca ctttctttat acatttctgg ctgctttctt tatacccgtc aactatactt
     3301 cacttatcaa taagaaagaa ggataagtat ggattttgga aatgaaacgg tcttgaaagc
     3361 aactggtata accaaaaaat acggtgctgc caaagcatta gataaagtat cgatcgaaat
     3421 caaacgtgga atgatctatg gtttgattgg ggagaatgga gcgggtaagt ctacctttat
     3481 gagaactatt atgggtttga tctcgattga tgaaggttcg atcgaattat ttggaacgac
     3541 ggatttgcag gcagcaagac gacgtatggg tcaatcgatt gaaacccctg ctctttatcc
     3601 agaattaact gcaagagata acctacgtat ccaagcagcg aacggcggtg tgtcggatag
     3661 agaaatcgaa gacctgctga aaatgatgcg attggaaaat acagggaaaa agaaagcaaa
     3721 gaatttctct ttaggtatgc gccagcgttt agctattgca aatgcactga tcacgaatcc
     3781 agaatttttg attttggatg aaccgacgaa tgggatggat ccagcaggga tggcagaaat
     3841 gcgagaaatc atccaacgtt tggtaaaaga acggggcatc accgtcttgc tttctagcca
     3901 cttgctagat gaattgtcac agatcgccac tcactacggt attttgcatg aaggacactt
     3961 gatcaaagaa ttatcgaaag aagaattggc acaagaatct cgtcaattta taaaaattga
     4021 cacttcggcg acggaacaag cagtaacggt tctcgactct cttggttata gggattattt
     4081 tgtccaatct agccgtgtta tccagctttt tgaaggaatt gaccaagtag cagccatcaa
     4141 tcaagcattg gtagaggcaa aagttcctgt ggatggtatc cacctagttg ggcaaaaact
     4201 ggaagattat ttcttacaat taactggagg caatccacat gtttaatcta ttaactgctg
     4261 aaaaaataaa agtatggaag agccgtaaaa tgtgggtctc actaggactg atggtcgtac
     4321 ttccgctttt ttattcttgg aacgaatggt atatgcatac aaaatacggc aatgaattaa
     4381 accaagcaac ggatacagtc atcaacggtg caacagggat tttaatggtg gaaaaaatgg
     4441 ctgcatggat tcttctggct ttcgcagcat ttgcgtgctt ttatattggg gaagaatttc
     4501 aaaacggtac gattcgcaat acgttatctt taggacggaa tcgaatgact tattatgtgt
     4561 cgaaacttct cgttacgctt ttgatcacaa cacttggcgt ggttttgatt acggggctag
     4621 ccatgattgc ttacacactt gctttcggat ttggtgaagt tgagggaatc aaaaactatg
     4681 gaaattatgt tctaaaagta tttcctgtat tgcttctgct gatattagcg acattatctg
     4741 ttccagtggc tttgacattc atcactagaa gtaccagtgt cagtttgctt ctttcttttc
     4801 tttatatcat gggaacggcc ttcgttcccg gtgttttcgc aaaaatcaaa ggcttggaat
     4861 ttctaacgga gtggtttacg gaaacgtggc tcatgtatac ggactttgcg caacaagcaa
     4921 cttattcgca agcaccgaaa atgattctgg tcagtctagt aacaatcgtg gtatcatcag
     4981 ctctaggaat gtttattttc caaaaatctg acattaagta ggtgttcctg ctggaaacta
     5041 ttttgattat cgaagacgat gaagctgttc attctttact ggaggaggtt cttgaacaga
     5101 gatacaaact tcttgatgct tattcaggta cggaaggaaa attattgtta agcacatatc
     5161 ctgtcgattt gatccttcta gatctcatgc ttccagggct ttcaggtgaa gcattgttag
     5221 cagaaatccg acagacatcg aatgttccga tcatcgttct ttcagcaaaa agcgatcaaa
     5281 gagataaagt ttccttgttg gcagcaggag cggatgacta tgtgacgaaa ccatttgata
     5341 tcgaagaact gttgttacga atcggcatcc aacttcgtca tcagacttct gtccaagtaa
     5401 aagatctgtc gcaacgcatc tattacaagg aaatcttact ggacaaagag tcgagggatg
     5461 taaatgtgaa tggtgtggct gtccatttga caggaagaga attcgatctg ctgaagcttt
     5521 ttttagaaca tccgaaaaaa gtattttcgc gagcaaatct ttatgaaaca gtttggcaag
     5581 aaccatattt tgatagtgag aaaacagtga atatgcatat ttccaatctt cgagggaaac
     5641 tagcaaaggc acatgctgtg tatattcata cggtgtgggg agtggggttt agatttgatt
     5701 aagtggctgt tgcttctcgt tgttatcttt atgggaagtt ttattttctt cctgcaaagg
     5761 gaaatcaaga gattaactaa gaaaatcgcc actctttctg aacgacaaga atatggtggt
     5821 cgtctttcag tcgaatttcg agaaaagaat ctaatggacc tagtagatgc actgaataca
     5881 tgggtggatg aaaatgaaca aaaaggacaa gcatttcaag aaatggaaga aaacatcaga
     5941 ctatcgatcg tcggcctttc tcatgatctc agaactcctt tgactgctat taacggttat
     6001 gtacaattac tggaacaggt agaggatacg gagaaacggg agaagtattt aacgatcatc
     6061 aaacaatctg ttgcaagatt actcagtatg acggatcagt tttatgatct tgctcgaata
     6121 gagacgaagc aaaaagaaat ggatctccat acgctttctt tttctaaaga ggtggaagaa
     6181 gtatttttct ctttttatga acaattttct gagaaaaata tcgaggtagc gttcgctgaa
     6241 cactcgataa atacaactgt attagcagat ccgctcatgt tgctgcgagt gctgcaaaat
     6301 atcatacaga atttattgcg ttatgcagat ggacaagcaa aggtgacatt ttcaaaagag
     6361 gattcattca ttcgattaac agtggccaat cctatcaaac caggagcgca aacctctgtt
     6421 gaaaaagtct tccagcgttt ttataccgaa aataccagcc gcacaaatac ggaatctagc
     6481 ggaatcgggc tttacctgtc aaaaaaattg gtggaaggaa tgggaggaga gatgaccgct
     6541 agattagacg ggggcatctt ctccatctcc gtcaagttgc ggagggttta attggctcta
     6601 atttacctga agtgaatgac aaaaaattgt atctcgtttt aaagacatct tcttttttgg
     6661 aaggtgtttt tattttggtg tgattgatac gaattaggta acgaagacag ttatttttgt
     6721 accccctttt caaatagcgc aacagtgcta tactaaaact aacaaatcaa ggaacggaga
     6781 gggatatggg ccaaagagaa tttatcgttt taattattat tgcagttgtt attttattag
     6841 ttgttttaga cattttttca cgtttgaaag taaaggaaac agttcgtagt aattggggaa
     6901 aaattcctta tcaaccgaga tttgataaag aagaaagttt gaaggatgcg tggctcacgg
     6961 agaaaaaatt ccgttcatgg gatagtgaaa tcgacgatct tacttggtat gacttggata
     7021 tgttcgaagt atttgaaggg atcaacagta cctattctag tgtaggttca gaagcgttgt
     7081 atcagcgttt acgtagcttt gattttggag aagattatca gttagaaaaa ctaattgctt
     7141 tttatcagga aaatcctcaa ctgagagaaa gaatccaata tcaattcgct cgtttaggaa
     7201 aaaaagatca taattttgca aaacaatatc tagcggatgg aaaaagtaaa aagattggac
     7261 gcgtaggttt atttattttt cttggcttgc ttccgctggt gggtgtaggt ttgcttcttt
     7321 tagggcaagt actaggaatc tatctagcaa tcggtggctt agtattcaat acggtctatt
     7381 atctagtgaa gaaacaaaca ctagaaacag agctgaatag catgagttat ttggtttcaa
     7441 cgatttcagc cgccaatcag ttagcaaaaa tgacgacacc acttcaaaaa gagctgaaac
     7501 agcaaactaa accattaaga gacatcccta aattaggttt ttcatttcga atgaagagcg
     7561 ggagtgaagc agaattgatt tttgaatatg tcaacatcat gttcatgctg ccatttatct
     7621 cctatcattt cgttctcact aaattagaaa agcattcaac tgaagccatc aagctgtggg
     7681 aattgctggg agaattggaa gttgcagcag ccgttttgaa ttatcgcacc tatatgccga
     7741 tcacttgccg accagagttt actgagggcg gtgtgacagc gaaagatatc tatcatccat
     7801 tgttaaaaga agcggtagtt aatccagtca actggcagaa gaatacatta gtcacgggtt
     7861 ccaatgcatc aggaaaatct acgtatgtca aaagtatagc catcagctgt attctagcac
     7921 agacgatcca aacagcgatt gcagaacagt ttaccctgca acctggacat gtgttgactt
     7981 ccatggcaat cgaagatgat ttatttgaag gcgacagtta ttttgtttca gaaatcaaat
     8041 cgatcaaacg tttgctggac caagtggctt ccaaagaacg ctgctactgc ttcgtagatg
     8101 agattttaaa aggaacaaat acgatcgaac ggattgcttc ttccgcttca gtcgttcggt
     8161 ggcttgcgga gtatccatct ttagcatttg tcgccacaca tgatatcgaa ttgacagaga
     8221 ttttaaagaa ttattgtgag aatgtccact ttgaagaaca agtaacagaa ggaaaaggaa
     8281 tcagttttga tttcaaatta aaagaaggac cttcaaggac ccgaaatgcg attgccttgc
     8341 tgaaagtttt gaattatcct aaatccatag ttgaacaggc ccaaaaagaa agtttgttgt
     8401 tcgatgaaca gcgtcagtgg tatccgtttg attaacttta tcaaacgaag catgatgcgt
     8461 gggtccaaaa agacgattat caaaagaagt acattgatgc caaaaaagtg gcagaattca
     8521 atgggaacga gtctgctgtc cagatgatct tggaagattt agcaaaagac gatacaaaat
     8581 aaaccacgct gtcttccttc tagcagcttt tatcgaaaaa acttagcatc agcgacgaga
     8641 cgcacgttcc atggcaaagt tctccgtaaa acaaacagcg aaaacagaaa ttgaaaataa
     8701 ctatttccat tttatttatt tcggttaaaa taaaagaaaa aagctttcga taaaaggacg
     8761 gaggaaagct taacatgaat aaaaaagaca agaacctcag ttataaggat cttctatatg
     8821 tcatcgtagt agttgctgtc ggtactttta tgattttaaa agttccagag ttgtttagat
     8881 agttattcta tcgaagatga tcatgaacta gaaaacacat ctcctctttg ttagcaggtg
     8941 tgtttttgtg tggaagccaa gttgtttttt catctgctgt attccatgat acaattaaga
     9001 atcatgtcta taaggagata ggaaataaat ggcaaatgcg cttgaaataa ataatttgaa
     9061 aaagatctat ggaagtgggg tcgaagcatt gcggggaatc gatctttcgg tgaaagaagg
     9121 agatttctat gcgctattag ggccaaatgg agctgggaag tctacaacga tcgggattat
     9181 tacttcttta gtcaataaaa catctggaaa agtgaatgtc ttcggttatg atctggacaa
     9241 ggatctagtt cgagcaaagc aacagattgg attggttcca caagagttca attttaaccc
     9301 ttttgaaacg gtccagcaaa tcgtcgtgaa tcaagcaggc tactacggtg tctctcgcaa
     9361 agaagcgatg aaacgcagcg aaaaatattt gaagcagtcc aatctttggg aaaagagaaa
     9421 tgtccgtgca cgtatgcttt ccgggggaat gaagcgaaga ctgatgattg ctagggcatt
     9481 gatgcatgaa ccacgtttgc tgatcttgga tgaaccaaca gcaggagttg acatcgagtt
     9541 gagacgcgag atgtgggagt ttctacgaga attgaacgaa aatggtacaa cgatcatttt
     9601 gacgacgcat tatctagaag aagctgaaat gctttgtcgc aacatcggaa tcatccaatc
     9661 gggtgaactg atcgagaata caagtatgaa agagcttctg tcaaaactgc aatttgaaac
     9721 atttatcttt gatttagcgc catatgatac caaacctgtg attgaaggat atgcttgtac
     9781 atttgaagat gagcagacaa tcgctgtcga agttgaacga aatcaagggg tgaacggaat
     9841 cttcgatcag cttacaagac aaggaatcaa agttctttct atgaggaata agtcgaatcg
     9901 tttagaagaa ctgttcttga aaatcacaga agaaaataat catacggagg agaaaaatgt
     9961 ttaatcttta ttttactgca ttaaaaagtt tagctatcaa ggaaacgaac cgctatttgc
    10021 gtatatgggt ccaaacactg gttccgccag tgatcacgac atccttgtat tttgttattt
    10081 tcggaaaaat gatcggtggg cggatcggtg acatgggtgg tttttcatat atggaattca
    10141 tcgttcctgg tttgattatg atgtctgcta tcactagttc gtatgccaat gtttcttcgt
    10201 ccttcttctc tcaaaaattt cagaaaaata tcgaagaaat cttggtagca cctgttccta
    10261 cgcatgtgat tatttggggc tttgtgattg gtggtgtggg tcgcagtatt ctcgtcggct
    10321 ctctcgtaac gatcatttcc ttgttctttg taccacttca tgtgtattca tggttcatgg
    10381 tcatcattac attattgatg acggcaatct tgttttcatt agcaggactg ctcaacggga
    10441 tttttgctca gtcttatgat gatgtatcga tcgtgcctac atttgtcttg cagccgttaa
    10501 cttatctagg tggcgttttc tatgccatct ctatgttacc gcccttttgg caagctgttt
    10561 caaaagtcaa tccgatcgtt tatatgatct ccggtttccg ttacggattc cttggaacga
    10621 tcgatgtgcc agtagcaaca tcgatgatcg tacttgtgtt attcatcgtt gttttgtacg
    10681 ccgttacttg gtatttgatc aataaaggaa gaggattgag aagttagaaa agaggttgtg
    10741 aaatcagcgt tttgacctga gtattagaga aacaaacggg aaaatagttc ctcatatttt
    10801 gttgtttttt gagcgaatac cgagggtcag ctgattgaac accgtttagt ggagaggttg
    10861 tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc tcctcctatt
    10921 tttcgtttgg ttggacttat taccgcagat gcaagctttt gaacaccatt tattccggaa
    10981 aatggactgt gataagattt ttgtcacagt cttttttttg ctcaatcaga aacatctttt
    11041 aagctgttca ctttgcttct ttttttgttt cctagtataa tgacaatgtt tttgagaaag
    11101 attgcgactt cactgcttga tagaatggag agtaacaaat aaatgactac taatttttgg
    11161 aaagagctgc caaagccttt ttttgtttta gcaccaatgg aagatgtaac agatgttgta
    11221 tttcgccatg tcgtaaagca tgcagcagca ccggatgtct tctttactga gtttacaaac
    11281 tcagacagtt tctgtcatcc agatggaaaa gacagtgtac gaggaagatt aacatttaca
    11341 gaagatgaac agccgatggt tgcccatatt tggggagaca agccagaatt ttttagagaa
    11401 atgagcattg ctatggcaga aatgggcttc caaggaatcg atatcaacat gggctgccca
    11461 gtccctaatg tagcagaacg tggaaaaggc agtggcttga tccttcgtcc agaagtagcg
    11521 gcacaattga ttgaagcagc taaagctggc ggtttgcctg tcagcgtcaa gacgcgtatc
    11581 ggctatacgg aaatgtctga gatggaagaa tggatcagtc acctacttca tcaagatata
    11641 gccaatcttt ctgtccattt gcgtacgcgt aaagagatga gtaaagtaga tgcacattgg
    11701 gaagtcattc ctaaaatcat tgctttacgt gaccaaatcg ctccacaaac actaatcacg
    11761 atcaatggag atatccccga tcgccagaca ggagaagaat tggccgagaa atatggcgtt
    11821 gatggcatca tgattggccg agggattttc aagaatcctt atgcatttga aaaagacccg
    11881 agggaacata cagaaaaaga actgatcgga ctacttcgtc tgcaactaga tctgcaagat
    11941 cattattcag aaatcatccc gcgttccatc acaggactac atcgtttctt taaaatctat
    12001 atcaaaggat ttccaggagc aaatgacttg cgagttcagt tgatgaatac gaaatctact
    12061 gatgaagtac gagagatctt agagaccttt gaaaaagaac atccaacgct tttcagtgaa
    12121 caatgatttc ttgcttgaac accgtgaatt agaggtcgtg aaaaagctgt cctgtatcaa
    12181 gtaataagaa cagccaaaca aaaatagctc ttcatatttt gtcgtttttt gagctaatac
    12241 cgagggtcag ttgattgaac accgtttata tcagaggttg tgagcctgcc gtttaactct
    12301 gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt tgatgaatat tttgacttgt
    12361 gaccgaagag ttggtaggag aacaccgttt ataagaggtt gtgacaaaag tcttgtcgca
    12421 acctcttttt taaccaaaaa agtgcaaata gtttacttta agtaagagaa taattatgat
    12481 gaataaaaaa ggtggaacaa atagcatact atatatattg aaatgaacac tgtatggatg
    12541 ctccaacttg atgaagctaa ggaggaattc gtatgtcaga aaaggaatta atgaaggtag
    12601 tcggttttta tgaagggttg ccgatcgatg atccaaaaag ctttattgat gaaaaagaac
    12661 agatacctgt acccagttca cgggatcttc tggtaaaagt caacgcagta tctgttaatc
    12721 ctgtcgatac gaaacttcgc caagataacg ggataagaaa tgctttacgt atcttaggtt
    12781 ttgatggagt cgggaaagtt gttgctgtag gtgacgaagt gcaaaaattt tctgtcggcg
    12841 atcgcgtttt ttacgcaggt acaacaagag caggaagcaa tcaagaatac caactagttg
    12901 acgaacgtat tgtcgctctt gcaccaaaaa atctttcaga tgaagaagct gcagccttgc
    12961 cgttgacctc attgaccgct tatgaattgt tatttgaaaa atttggtcta actccagaag
    13021 aaaatgccaa tcgcgggaaa aaaatcttag tgatcaacgg atccgggggc gtcggttcga
    13081 ttttgaatca actagcgcat tgggctggcc tagaagtcta cgcgactgca agtccgaaga
    13141 attttgaatg gctgaaaaaa acaggtgtag actatccaat cgattatcat cacgacttaa
    13201 gtaaaagttt tcgtgaatta ggaatcgatc aagtagagta tgtcgctgta ttattcgata
    13261 ttacccgcta ctttgatcaa atcaagcacc ttgtccgacc atttggacat gtgggaacga
    13321 tcgtagggat caaagagccg ttagatatag gtgtttggaa aaaccaatct atcagcttcg
    13381 actgggagta tatgttcgct aaaacagact ataactatga aatcgaaacg caaggagaag
    13441 ctttagcgca cattgctgct ttagcaaatg aagggattat ccgcagtaca ttgagcaagg
    13501 tctattcaga tgggatgaat gcgaagaact tgaaacaagc aaccaaagac gtagaaacag
    13561 gacatatgtt aggaaaagtc gttgttagtg gtccgttcaa cggataagca agaatacgcc
    13621 gatttttttc ttatttgact ttgataaaaa caagagcgca tgacagtaat ttgccataag
    13681 ctcttgtttt tttatttata ataagcgaat caacgaaagg gaaagaattc ctgccaagac
    13741 gatcaccaat aagctttttg aaagaatagc agctaaaagt gtaggaactg aagccaaagc
    13801 attttcgaga ttgatctgcg gcagatgtcc gatattttgg gtaaatagac tgctgaacca
    13861 taaagcagac ataatgacga tcgggacaaa acttaaatac tcaagcaacg gttgcggtaa
    13921 atcgaatttt ttcagtaaga caaatggtag gaccctagaa agccaagttg ctaaagtaca
    13981 acctaaaata gtcagcaatg taaactcaaa agaaggcatg tttgatccac acccccaatc
    14041 cgcacccaat cagtgttaca actacaatca ctaagttgct tgggataaaa atcagtccga
    14101 catataccag aatcaaagtg agaccaatca ggatcaactg taatcgttta gaggtgtttc
    14161 tatcagaaat gacttgaaga taaagtaacc cgataaacat agcgacgatg gcgaaatcca
    14221 aaccaaactt tttcggatca gtaataaaat tccctaaaag tgcgccaaca agagaagaac
    14281 ccacccacgt caaataagaa atccaattag ctgtattcat ccaacgaaag tttaactttt
    14341 gtccagtata gttcaattta ttcattccca gtgcgaaact ttcatctgtc agcaatgtgc
    14401 caatcagcag attttgaagc agccgatttt tcttgaaata aggagcaatc gtcatactca
    14461 tcagaatcat tcgggaattg actagaaagg tcgagaaaac gatggaaagc aatgggctgt
    14521 gactagcaag catgctgact gtgataaatt gggcagaacc agcatagacc agcagggaca
    14581 tcaacgtgac gactagtgga tgaaatccgg cagctttccc aacgatacca aatgcaagtc
    14641 caataccgat ataaccgaaa acggtaggta acgtgtcttt gatcgcagtt tttatatcta
    14701 acttttcatc caataacaaa actcctttag cacgacataa attttcataa ttgtcgttaa
    14761 acttgtgaat attctaaatt tttaatgagc atttgtcaat ggtgggattg aaacgtaaag
    14821 aactagcggg tcgcagattt acacgtattg tttcggtggg aggaggcgcg aaaaatcttg
    14881 catggctaca aatccaagct gatattttcg atgcaacggt ctattgttta agtgtggagg
    14941 aaggacctgc attaggcgca acaatgattg ctgcactagg gttagggtgg ttcgacacac
    15001 ctgaaagttg ttgtcagaat tttatccaat atacagatgc agtggaacct ctcttacaaa
    15061 atgtagaaaa gtatcataaa atctatcaac aatgccggaa aatctatcca gcaacgaagg
    15121 aaatttgtca tgacttataa aacaaaacgc gtacctaata gtcaaatgac taatattggg
    15181 tacgcgttaa gtcttcttta cttcatcctt tgcatgattc gtgtttttaa agcaaggtct
    15241 gctgtatttc ttgtggattt aggatggttg ttatcgttta cttgctattt ttcattgaaa
    15301 tcgtctttct ttaccgtttc tttagttttt tatctatttt tttcttttgg ttagaaaatt
    15361 ctttttcccg aaagtcttct ttcaggattt ttatagagta gcgttttgaa taagaggcgt
    15421 cagtatgatc gacttggcag acgaaccaag agaacatttg ccttttttct gcatgaggtg
    15481 cttctgctaa taattctttc cataaataat aacagtcctc tatcagaaga agggttcgtt
    15541 catcctcagg ctcccaatag aatgtttcga gagtagcaat cacttcattg atcaattcga
    15601 atgcgggaag ataggcattt tgctgaataa gcgattcaac tgcttcttgg atgaactttc
    15661 gcatctccat gatgaacttc gctgttttcc tgtttttgtt tttcttgata atatgatcgt
    15721 atttatggat gatagttgtt aatttatctg aataatttat tgcatgaaat ggcatcttaa
    15781 ctttgaagcg ttcgataaag tgcggatgtt ctataagaat ctctgttaaa agctcccgta
    15841 caagagagga actagcggag gaaacaaggg acgagagttc ggaaagtgag tagttggatt
    15901 tttttattgg acgttcttcg taagcgaata agacagcagc catgtgtttg catttttttc
    15961 cttctatggc atatagacaa tcacaagtca tgttggtctt ttcatcccaa gtgatcacta
    16021 ccttataatc ttccgtgcca gaaactgttg cggacaagta cttgcttgta tagttcaaat
    16081 gacggacaaa accgtgcaga tgatagctgt aaccgcgttc caaaacgttt tcaggaaaat
    16141 actcaagcca attattcatc tagttctcct ttcatgaaac acttatttat atttagtata
    16201 aagaaacgtt taagaaaaaa gtagctgatt ttacatattt gataatcact tttgctcgaa
    16261 ttgtcaaaaa ttcattctcc ttttcttttc ttagagaatg tgagaaaaag taaaagaata
    16321 gcgtgtttta aagatggaac tttttttgtt ttttatttat ctgacaaaaa tgaagtgatt
    16381 ttgttagaaa taaataattg ttccaattga taaaagaggt atgatgtatc gcaaaga
//
LOCUS       DAFBQW010000060        15858 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFBQW010000060 DAFBQW010000000
VERSION     DAFBQW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.063"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            212..1381
                     /locus_tag="LK889_002214"
     CDS             212..1381
                     /locus_tag="LK889_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5096420.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1706..3025)
                     /locus_tag="LK889_002215"
     CDS             complement(1706..3025)
                     /locus_tag="LK889_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5096421.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(3022..3675)
                     /locus_tag="LK889_002216"
     CDS             complement(3022..3675)
                     /locus_tag="LK889_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5096422.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            3963..4896
                     /locus_tag="LK889_002217"
                     /pseudo
     CDS             3963..4896
                     /locus_tag="LK889_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4804..5520)
                     /locus_tag="LK889_002218"
     CDS             complement(4804..5520)
                     /locus_tag="LK889_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBK5096423.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(5570..5779)
                     /locus_tag="LK889_002219"
     CDS             complement(5570..5779)
                     /locus_tag="LK889_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096424.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            6056..7329
                     /locus_tag="LK889_002220"
                     /pseudo
     CDS             6056..7329
                     /locus_tag="LK889_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7436..8467)
                     /locus_tag="LK889_002221"
     CDS             complement(7436..8467)
                     /locus_tag="LK889_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096425.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8474..9304)
                     /locus_tag="LK889_002222"
     CDS             complement(8474..9304)
                     /locus_tag="LK889_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096426.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(9301..10116)
                     /locus_tag="LK889_002223"
     CDS             complement(9301..10116)
                     /locus_tag="LK889_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5096427.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(10113..10937)
                     /locus_tag="LK889_002224"
     CDS             complement(10113..10937)
                     /locus_tag="LK889_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBK5096428.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(10927..12027)
                     /locus_tag="LK889_002225"
     CDS             complement(10927..12027)
                     /locus_tag="LK889_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5096429.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            12205..12990
                     /locus_tag="LK889_002226"
     CDS             12205..12990
                     /locus_tag="LK889_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBK5096430.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            13023..13406
                     /locus_tag="LK889_002227"
     CDS             13023..13406
                     /locus_tag="LK889_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK5096431.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            13482..13613
                     /locus_tag="LK889_002228"
     CDS             13482..13613
                     /locus_tag="LK889_002228"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5096432.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            13582..13818
                     /locus_tag="LK889_002229"
     CDS             13582..13818
                     /locus_tag="LK889_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5096433.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(13896..14867)
                     /locus_tag="LK889_002230"
     CDS             complement(13896..14867)
                     /locus_tag="LK889_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK5096434.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            15519..>15858
                     /locus_tag="LK889_002231"
     CDS             15519..>15858
                     /locus_tag="LK889_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5096435.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
BASE COUNT         4889 a         3358 c         2748 g         4863 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac tcaaaaagat agcgtaaaat
      121 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
      181 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
      241 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
      301 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
      361 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
      421 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
      481 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
      541 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
      601 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
      661 aagaacgtta gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa
      721 gcgacagact gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac
      781 caaagaagtt cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt
      841 tcttcaagat cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt
      901 aagctgtatt gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg
      961 tgtgcatgta tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg
     1021 tgaggatttc aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga
     1081 ctttatgact aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc
     1141 tgccctatta accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa
     1201 cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc
     1261 taatgaagaa tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt
     1321 tttaggtaga attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta
     1381 actgtaatta acagaatgga ttttccattt acacataatt cttgacgcta ccataatcta
     1441 aataaccatc atattcatct actacttata taattgagtt ttattaaact ttttaagttt
     1501 ctattctatc aaatatttaa acaatctctg catccgaaaa gactacctcg tatagtttta
     1561 ctaacaatat ttttattagc gtgttcaaat cctgatttct tgtatcaatt attttctgtt
     1621 cactttcaat aagaaaaaag tctttacttc catggataat aataagtctc tatttttcaa
     1681 taactattgc aaaattatat cttaattaag aaaatatttt tattttaaag ataaaaaatt
     1741 cttcatcctg caatacttta tattctatat tgtaattatt cagaatatta cttacaatat
     1801 ataaacctaa tccattacta ttttccttat ttaaatcaaa tttaacatca aatattttat
     1861 ccatattcga aattttattg ttaccgtatg aattctctat atataaccaa tcattaacta
     1921 tcccaatatt aattacccca tttacatcag tatattttac cgcattacta atcaaattag
     1981 atagaataat ctttaaagct gtttttccta tataaacatt ctcgtctaat atataattat
     2041 tgacagttat tttcttttga tttgctaata tttcatactt ttttaatatg tcttctaatg
     2101 tatcatttat tttcaaatat tcttcatcat tattcaaatt ttctatagaa tgaactgata
     2161 atatttgaga aatatttttt gttaaactat caacgatttc aatacattca ttaatataaa
     2221 tatctctttc tttatatttt ccaatattgt atttcatgtt ttccagtatt attttaagac
     2281 tagcaagagg ggttttaagt tcatgggatg cacctttaaa aaagtcatat tttaactttt
     2341 ctaattttaa aatttcttta tttttaaatt ccaaatcgtc aattgttctt aataaagtag
     2401 aatacaaatc attaatttgt tgttttaatt ctccaacttc atcattagag ctaactttta
     2461 aactcatttt tttatcaagt ttcatcattt tatcagtaac aatttttatt tcttgtatat
     2521 tattttttat tgattttgca taaattaaag aaataatagc agaaaataaa attgatatta
     2581 ataaagaata tggtaaaaat ttaaggctta aatctttcgc atctttttgc atatcagctg
     2641 tagaaacaaa ttgtagattt gtttttttac catcattaag ctttatttct ctttcttcaa
     2701 ttatcaaaga attactatca ctttctaaat taacatttat attatctttg atttgtattt
     2761 cattcttatt atttttttct tttatgaata cttttatttc actactcttg gaataaaact
     2821 ccaaagtttg ttctatatat tttatttctt ttccattcat attactagaa atttcatttg
     2881 ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa
     2941 ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt atgaacattt
     3001 ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt
     3061 agttatacag tctaattgta atttttttct aagttctttt atatatacat ctattactcg
     3121 atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa
     3181 cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg catttacatc
     3241 tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata
     3301 ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa
     3361 gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat
     3421 tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct
     3481 aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa
     3541 atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt
     3601 atatccaaat tctgaaagat attcacttat tccctctctt atcatattat catcttcaac
     3661 agttaatatt ttcatatata cctccactta agcctataca ctagatatta tacagcatat
     3721 tatggctcta taatatctag tgttggtgac gccttctgtt gccaatactt tttctttttt
     3781 ttaataaatg caaacagaca ccttgaaatc ctttagaaaa aaggcttcta taatattttg
     3841 tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa
     3901 agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct
     3961 ctatggataa tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg
     4021 aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat
     4081 tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt
     4141 cctatacgac acgtactcag atgttgaaag tcaaagaaaa gttgactatt ttagaattaa
     4201 aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag
     4261 ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg
     4321 aaaatcaatc acgtaaacta atcgctaaga agtacttcgt ctcggatggg acggtgacac
     4381 ggatcttgcg tgaagcaacg aagcattacc agccacgtat gaacttcctt ccgacggtgt
     4441 tgtgtatgga tgagttcaaa tcaatgaagt ctgtttctag ttccatgagt tttatttgtg
     4501 ttgatgggac aacccatcag ctatttacga tccttgaaga tcgcagactt tacaaactaa
     4561 ctcaatactt cctgcacttt cctagaaaag cccgtttaaa ggtcaagtac ctagaatgcc
     4621 agctattgtc agctgcttaa aacggtcttt ccaaatgcgg agatcgtaac tgatcgcttt
     4681 catattgtcc aacatatcaa tcgcagcttc aatcaattgc gtatccagat catgaatcgt
     4741 tttcgagcct cgcactctga aaatcagaag aagtaccgtc ggctcaagcg atattggaaa
     4801 ctcttactca aagattccac gacattggag ccgctcaaac ggcattatca tcgactgttt
     4861 aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt tgtgcacaat ggtgtcctga
     4921 acgtacagcg acaccttcca tatccagagc agtcgccaca tcacggggat ggatgccatc
     4981 caaattaaaa gcaaccacgc cagtatgatg ttcaggatct tgcggtccgt aaatggtgat
     5041 accgccgatc gcttgtaatt taggtaaaac gtaggcaacc agttcttgct cataacgatg
     5101 aatattctcc ataccaatct cattcaagta atcgattgct cctgcaatgt tcggagtacc
     5161 agcttcaaat ttccatggca gctctttcca tgaactatcg taaagattga caaaatctat
     5221 catttcaccg ccgaattcga ctggctccat ttgttctaac aattgacgtt tgccatataa
     5281 tacaccgatt cctgtcggcc cgcacatttt atgcccgcta aaggcgaaaa aatctgcatc
     5341 caacgcttgt acatcaactg tcatatgcgg tgctgcttgg gcaccgactg gactatcctc
     5401 aactacaaca gcctgttcaa ccctcagccg tttcaagcac aacaaataac cttctggatc
     5461 tggtttgccg acctgaagat catcgatcgt cacaaaggaa tcaatttttt ccttaatcat
     5521 cggatgtgct tcaaaaaaat agtctagctc ccgcagttca gaattgagac tactccgatt
     5581 ttccagtgct gctgatccgc tgctcaaaat gttcagcatc cactggtctc ccaaccgctt
     5641 gacttatccc ttcataaaag gacagttctg ctgccgtccc aacaccttgt tgatagtctg
     5701 caaaaggaat tttatagccg aactgctcca gtaaataggc ttcagtaaat agtgatgaat
     5761 actctcgtaa aacatccatt cactgtcaat catcgtccca tcaaaatcaa aaataagtgc
     5821 tttggtcatc ttctttcccc cttacccaaa acaagcaaaa gctgtgtcct gctctctcta
     5881 cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt gaaagaaatg tggcgctata
     5941 atttctagga ctgatagtaa atcattctat cagtcctttt gagcataaaa aaacaaaaca
     6001 tacagaagct ccgttagaat tgaatcacca caacacaatc aaaggagcgt tctgtatgcc
     6061 tgatcttcat cttatcaaac tattactagg aatcaaagat ggaaacattc aaatcactca
     6121 tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt gtttctgcca cgctcattaa
     6181 aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa catgggctga tcgttaagaa
     6241 cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg aatcaacgga cttatcttgc
     6301 tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc tattttacgg cgaataccta
     6361 cattgttgaa aaaaactgtt ttatcgccaa acaagtacat tataaaatat tggaggaatt
     6421 gactgaaaaa caagcaatga cgacgatttc taaacattgc ggcgtttctt ggtcaaccgt
     6481 ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg aaaataaatt ggttgcctcg
     6541 ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa gtcggtccct attctttttc
     6601 ttgtatggat ggcgatactg ggaaattact ggatattctt ccttcacgta agaagaaaga
     6661 tctcatttct tattttatgc aatttgaacg acgtgcacgt ttaaacgtaa aaatactcgt
     6721 aacagatatg aacgcctctt atgctttgct gataaaagag tgctttcctc atgccaaact
     6781 cgttgtggat cgctttcaca tcgtcaagca cctcattcgc agttttaagg atatttgact
     6841 atgagtcatg aaaagcttcg accgaaacga tccaatacaa gccaaacact atcgacaagt
     6901 gaaggcttta tctcgacttc tgatcaaacg acaggacacg cttgtttatg ataaatggac
     6961 aaaatggcgt aattttggtt gggcctattt aaccgaaagt gaagtcgtag aacgtctact
     7021 ttctacttct gatgaactac gaatagctta tgcttactat caagaaattc ttcaagcttt
     7081 ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa acaatgccca agtcggttcc
     7141 aagagaactg catcatataa aaaaagcgtt tataaactat gaatccggca ttcgtttagc
     7201 cttagagttg ccttactcaa atggcaaaat agaaaatctg catacccaca tcaaagcatt
     7261 gaaacgaata gcttacgggt taggatgaga accagaattt tcttaatcaa tcatctgatc
     7321 acttattaaa cataaaaaaa catctaaagc agaaaatcaa ttctacctta gatgttctta
     7381 tatcactcta tcagtcctat ttgacaaaga gccagaaatg tttgccgact atacactagc
     7441 tttgttgtgc aaaatgctga gtatcaattt ccacagggat tgctagaata tctttcagct
     7501 gaatgtataa ggcatcgcta attttctctt cccgatcgtt caaccgatcc actaaaattt
     7561 ctttgccctc cagcgtttga aaaacatacc gttgaatgtt gcctaaaata aatgattcct
     7621 tccatagaac cggaatctta accccctgag gaaccggttc aaattgaatc agctccggtc
     7681 ttaaataata aatgcagcga ttgcccttag cggaaaactc gtgggtatac tgattcagct
     7741 cataccattc gaaacgatta tagttgccga taaattccgc aacaaatcgg ctctgaggat
     7801 tctgatacaa ttccttcgag gtggaaacct gagccacccg cccagcttcc atcacaaaaa
     7861 tccgatcact gattcgcatc gcctcttctt ggtcatgagt cacaaaaatc atcgtcatcc
     7921 ctagcgattg ctggatagcg cgcaaatccc gctgcagctg ttttctaatt cgggcatcca
     7981 aagcacttaa tggctcatcc agcagcagga ctttcggttc agtgaccaat gagcgagcca
     8041 gtgccacccg ttgtcgttga ccgccagaaa gaaactggac atttttatct gcctgctccg
     8101 tcatatccac caatgatagc atctcaaaaa ccttttcctg aatcacctca ggactcagct
     8161 tctttacctt caaaccaaag gctacatttt caaaaacagt catagttgga aacaatgcat
     8221 actgttgaaa aacaaagccg atattgcgat ccttcgtagg cttatcggca atattctcat
     8281 tgtccagcac aatctcaccg ctctttggtt gttccaatcc cgtgatacag cgcagcaacg
     8341 tagactttcc gcacccgctg ggacctagta aagtaaccag ctcacccttt tcaatctgaa
     8401 tttcatcaat agctaaaatc tgtttttcat caaaaaacag attgagatca tgtatttcta
     8461 aaaacatttt ccgtcacaac tcctttaagg gcttcttttt cgactgcttc tcttccctcg
     8521 tctgcttaag ggacagctgt tgcttttgtc gataagtcag cataaaggta gttaaagcaa
     8581 ttcccaccat aaacagcaaa tacaaaacca tcacagcgct agccaaatgg ccgttttcgt
     8641 tcatccgccg cagcatatag atgcgtaccg tctgaaatcg accgccaatc aacaggttcg
     8701 tcagcatgta ctccccaaaa accgaagaaa aaatcatcag agaaactagg gtcacaccga
     8761 tacggatgtt tggaaacagc acctggataa taatcgtaga catcggggcg cccaaggttt
     8821 ctgctgcatc cactaattcc ttcagattaa ttaagcgcat ctggttgtta agactaagat
     8881 agacaatcgg caacgccgta ataaacaatg cacctaccaa cactacaaac attgggatac
     8941 cagtttcagc atacgttcga agcaaagccg ttaccaaaat cactcctggt aaagcatacg
     9001 gcagcaggag cagcttttcc agccaacgat tcaaacgagg gaaatacaaa tgaatccaaa
     9061 taatggtggg aatcatcaac aacagaatcg tcaccaagac cacacctgct aataaaagtg
     9121 agcgacccac cgcagcaata aattccctgt ccgtcagcag ctgttggaac cattgcaagg
     9181 taaaatcact aggtaaaagc gattttgacc atttagtgga gctggcatac aaaatcgttc
     9241 caataatcgg cagcaagtaa tacaggacaa agcagaacaa aatcggtttt attaactttt
     9301 tcacgtctgc accaccttgc ctttcttttg aataatcaac attgccgtta ccagcaggac
     9361 acctaaagcg actgccaacg cactgcctaa attaggcttg gcgaacacat ctcccgacac
     9421 taaagccgca attcgaatcg ttaaaagatt tacattactg ctggtcaggg catacgctgt
     9481 ttcataggtc cccatgccat tagcaaataa gatgacaaag gtgctggcaa tcgatggtcg
     9541 caaaaaaggc aaagccacct tgaaccaagc atacgctgat gaagcgccca gcaattgtgc
     9601 ggactccagc cattcctttt tcacttcctt catgctggga tacaaaaaca agacccctaa
     9661 cggaatttgg aaataggtat acgtgatggt caatccccac caagaataca aattgaagtc
     9721 cgctagaaat ggcaaaatca ttttcagcac acctacattc cccaacaaaa taataaaaga
     9781 gaatgccagc ggaatcccag caaaattagc tgccaaattg gaaacaaagg tgattttctc
     9841 ctgcaattta gtcgatagat tcattaagca taaacataaa ataaacgtcc cgattaaccc
     9901 aatcacactg gagctgacag caatcagtac gctgttaaca aaggcctgtg tgtaatattt
     9961 gttgctgaaa atatcactgt aattggccaa tgtccactgg ttagttaaat cactgtgcaa
    10021 gctgcacaga accataaaaa gcagcggcag cagtaaaaat gcacagaaga taaaggaggc
    10081 aggaaccaga accaagcgtt ttttcatctc tttcatcccc tcatcaattc ttgaatgtcc
    10141 tgcattttct ctgacggtgc aattcctaat aaccagcaaa acaatggagc cgccagcagc
    10201 tgtggtacag tttgttcctt cagcttttgg cgtttatttg aaatcaaata aaaaggaact
    10261 tcacgatgtg cagccaagct gccgccatgc agtccgtgcg catccatccc gtgatccgca
    10321 gtcaccacga cttgataacc tttttgcagc cattcaggaa ggtaaatagc caataatctg
    10381 tccgcctgat taaccgctgc tgcatattca tgagaatcgg cggtaaattt gtgaccgaca
    10441 tcatcaatat tcatcgaatg tacgaccaaa aagtctggct gatactgatt cactaaataa
    10501 tgggcatccg caaataaatg cgaatcaggg taagtgtcct cactataaaa gatgccatgc
    10561 tgaatcagtc gctcctcctg atgctggatg cgatcaacca aacgattata gggggcttga
    10621 ttgtacaact cgctgtacca gtggtaagcg gcggctcctg tttttccgcc agcttcctta
    10681 actaattcaa acagcgacca ctctttggat cgctgagcat ggtaattact atagatgcca
    10741 ttttcatagg ctggaacgcc ggtcatcagt acttcgtata acggacgtga attgctgggc
    10801 agctcacttt ttaccttcaa taagcttgct tgctggtgct caactaaatg attcatgaac
    10861 ccaagctggg cgacggctgt gtcgtagcga cagccgtcta gtataactac aatcagtttg
    10921 tcaagtctac tttgcatagg tcagtacctc cgcctgccaa agctctggaa gctctacaga
    10981 ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc gcttcataag catcctccgg
    11041 cagtaatttt tcagctactt ctttaggcag ctctaccttg cgaataggtc gcgcatagcc
    11101 ctttgccaaa ttgatttgcc cttcatcaga taaaatatat tcccgtgcta gctttgccgc
    11161 attgggatga ggggcatttt tattgattaa ggttgtatac ccgctaatga tggagccgtc
    11221 tgttggcaca gttacctcaa aacgctcgga gtcaatttgg tcacgataat ttagtccatt
    11281 gaagtcccaa acaactgcca catcgatttc acctttttca agatttgcta cgctggcatc
    11341 aacggtggac aaacggcctt tttctgccat ttttttgtag taattcagac ctggctgaat
    11401 atccttttcg tcacctccgt tggcaatcgc cgcagccaat acagcaaatt gtgcttggtt
    11461 ggcaatcgtc acatccccca cgctaacttg atagtcaccc tcttgcagct ccttccagct
    11521 agtcggtgct tgcttcacat tctctttatc ggtgataaag gaaatggttc ccgtatagct
    11581 taacaaccaa tccccatcat catcctttgc ccaatccgga atttcctccc aatagttggt
    11641 cttataaggt aaggttaact ctttgctttc agccaacgga ccgaagctga taccgacatc
    11701 accaatatcg gcggttccat tttttccttc tgattcaaac ttagccaatt cttctgcact
    11761 ggacatatcc gtatccacgt gacttaagcc gtattctttt tcaaggtcgt tccaagtgcc
    11821 tacccaattt gcccaagtat ccggcattcc caagctggca atctctcctt cttccttggc
    11881 tttttctata atcgtatcta aagagtcgtc tgccgcagcc gattgcttcg ttgcttcacc
    11941 tgagccgcag cctgtcagca ggatacttac tgttgctgca caaaataaac cgatagtcat
    12001 gttacgaatt ttttcattca ttttcattca ttcttctccc ctttcgataa ttccattcta
    12061 acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt ttcatgaaca atgtaaaggt
    12121 tgtatatggt ttgtaaattt cgacgaattc ccgttaacct tctaaagctt ttgcggtatg
    12181 atagtagaaa aaaggaggct ccctatgcag tattatgatc agcatctcca cacgtatttt
    12241 tcctttgatt cagaggaaaa atttgaaaac tatttagcct atcagccgga gtttttcgtt
    12301 tctacggatc actttgattt aaacaatcca tctaccggaa aagatgacat tccggattat
    12361 ccagcctata taaacaaact ggactcttta aaagaaacct attccaccaa atttctgcga
    12421 ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga tcgtggatta cctcgctcag
    12481 catccttatg atttaaagct ggtaagtatt catcaaaatg gccgctttga ttatatggac
    12541 gatgtggttt taaccaaaga tcccattcaa actaccaagg aatattttga tcagatgttg
    12601 caggtcttga cggattttcc agaaggagat attttgactc actttgatta tggattacgc
    12661 cgcttcacct tcacagcgga agaattcgcg gagcattttg aggctcagct gctaacgatt
    12721 ttcaaaaagg ttgttgaacg agaattggcg atggagttaa atgccaagag cttcttgaaa
    12781 tacggcaacg aagaccttta ccgttatgcc gttcctttgt atcaaagtgt cggcggaaag
    12841 ctctttacct taggctcgga tgctcatgtt gccgaagatt atcagctggg ctttaaaaaa
    12901 atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc tagtcattca aggaaaagaa
    12961 cgccgcctga cgccgctgcc gattttgtga ttcctatata atagaagaaa gtggtgaaaa
    13021 aaatgagcaa cctgtgggac tacaatcaag aggcacccat ccattattta attgcgcgac
    13081 attgggatgc gttgaaaatt gaggcggttt gccgatcctt gttagcagcc gttccaaaac
    13141 aacaactgga aaattttttg gtggctgaca gcttgcagcg ggaaaaagtt caggcttatt
    13201 ttgcagcctt taaagatcag cctttggaat atttgcatgc gcagtttcat cttttttatc
    13261 aagtagccgc cccagatgac tacaacgatt tacgaggaca gctgcaattg acctttcaag
    13321 cggatgaaac cgcctatacc gttttattgg gaatggctcg ccttggtgat caggcgaagg
    13381 tcgagtggcg gattttcgat atttgatggc aggatcaagg cgtttcagtt gcaaaccttc
    13441 ctctctttca attagaaaaa ccgcagcagg aagaagtttg tgtgctggtc accgatgctg
    13501 gtgaaattcg tctccgctta tttcctgacc aagctcccct ggctgtagcc aactggcgct
    13561 ccctagctga gcaaggcgtt tatgacaaca cacctttcgg ctcgtgtcat taaggaattt
    13621 gttattcaag gtggcgcttt ggatggttct ggtgatgaag ctgtttccag ctatgagggc
    13681 tttttcgctg atgaagtgca tcgcggactt tatcatttta atggcgcctt ggccttaggg
    13741 gaccacggcc cccacaccaa cggcaaccag ttttttattg tgcaaaacac aaaggcgcag
    13801 gctgacctgc taatgtaaag caagcatact tggagcttgg aggactaccg gagctggatg
    13861 gtcgttatac tgtttttgga caggtatacc aagggttaga aatcgtagca gcgatcgcag
    13921 ctcagccgac aaacagtgaa gatgtaccga ttgagccagt gttcattcga caaattaact
    13981 tcgaaaaaac atcgtaaaaa gacactcact gacttcagca aaagctctat tcttgagctg
    14041 gctgcagcta gtgagtgtct ttcatttatc cataagataa agcttggatt caataaataa
    14101 ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa ccgtgcgagc cagttgatct
    14161 gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt ctgccaacgg atactccagt
    14221 cccaatgctt ggtaaccgtg cataccatag cagtggtacg gtagtaattc taacggtacg
    14281 aaccgatcaa gatctgccac aaaacaagca atcgtcagca gctcggtcag gttcatattg
    14341 atacctggaa ccacaggaac tcgaattcgc acttcgcagt taggatactc aattaaaaag
    14401 cgacgaatat tcttcaaaat ttgttggtta tctaagccgg tccattgttt gtggagcttg
    14461 ctggtaaaca tttttacatc cacaaacaat gtatccacat agggagctac cttttggatt
    14521 tcattataag caccacaaaa gctggtttcc agtacggtat tgattccaat gtatttcgat
    14581 ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg cctcgcctcc gctaatggtc
    14641 actccgccat ccgagtgaaa gtaaaaaaca gcatccttct caatctcatc cattacctct
    14701 tcagcggtca tggtctggcc aaatcctttt tcaatctgct tgctttgtga ttcgggaatc
    14761 gaacaccagg cacagcgtaa tgggcagccc tttaaataaa caacggtccg taaaccagca
    14821 ccatcatgta gagaaccctg ttctattctt aatactgttc cttccatata gatttttgaa
    14881 ttacacacta gtttaaacta aaacacacaa ggaaaaactt accatttaat cataaaatta
    14941 gctcatataa atatactagg atgtaaaata gactggtcaa aatctggtca aaaaagagga
    15001 ctaatacagg tagtcctctt ttttgttatt ttaatattct aaatcatttt ttattggttt
    15061 attcccttat tcgatgtaga ctcaaatcac tatatcacta gatatcaaac ggcgtcatac
    15121 ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt gaataacatt caaaatcaat
    15181 aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa aaaatatata cagccttgta
    15241 ggtaaagtac cacagggctg tatatttttt atttcagatt accttaaatg attccattta
    15301 ctttttatct cattttttcc tactagttag tatcaaatac tctcctattt tctcaataaa
    15361 ctatttttgg atacatggta tctgaaacag ctaaaacctt gtcattacag caaaaaatca
    15421 cgttatccag actgtagaca aagtccattt tatggatgat gtctacagtc ttttctttta
    15481 taatagattt atgaaaacga atgcaaggag tgatagctat gatgtctaaa aacaatacga
    15541 atggtagaaa tcaatttgcg atgcttacga ttgatgatct agtttctcaa gatcatctcg
    15601 ttcgaaagat tgatgccgcg cttgattttg aatttattta tccaatcgtc gaagccactt
    15661 actccgattt aggtcgccca agtatcgatc cagtgattct gatcaaattg gtgtttattc
    15721 agtatttatt cggtatccgt tccatgcgac aaaccattaa ggaagtcgat accaatgtcg
    15781 cttaccgctg gtttttaggc tactccttcg aggagaaaat cccccatttc tcaacttttg
    15841 gaaagaatta tgttcgcc
//
LOCUS       DAFBQW010000061        15471 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFBQW010000061 DAFBQW010000000
VERSION     DAFBQW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.064"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1126)
                     /locus_tag="LK889_002232"
     CDS             complement(<1..1126)
                     /locus_tag="LK889_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK5096436.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1209..1544)
                     /locus_tag="LK889_002233"
     CDS             complement(1209..1544)
                     /locus_tag="LK889_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBK5096437.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1554..1928)
                     /locus_tag="LK889_002234"
     CDS             complement(1554..1928)
                     /locus_tag="LK889_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5096438.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1941..2255)
                     /locus_tag="LK889_002235"
     CDS             complement(1941..2255)
                     /locus_tag="LK889_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5096439.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2368..5595)
                     /gene="fss3"
                     /locus_tag="LK889_002236"
     CDS             complement(2368..5595)
                     /gene="fss3"
                     /locus_tag="LK889_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBK5096440.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(6009..7175)
                     /locus_tag="LK889_002237"
     CDS             complement(6009..7175)
                     /locus_tag="LK889_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5096441.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            7200..7526
                     /locus_tag="LK889_002238"
     CDS             7200..7526
                     /locus_tag="LK889_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096442.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            7679..8731
                     /locus_tag="LK889_002239"
     CDS             7679..8731
                     /locus_tag="LK889_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5096443.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            8807..9025
                     /locus_tag="LK889_002240"
     CDS             8807..9025
                     /locus_tag="LK889_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096444.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            9012..11585
                     /locus_tag="LK889_002241"
     CDS             9012..11585
                     /locus_tag="LK889_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096445.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKF
                     DSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSC
                     N"
     gene            11576..11773
                     /locus_tag="LK889_002242"
     CDS             11576..11773
                     /locus_tag="LK889_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096446.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            11777..12388
                     /gene="mobC"
                     /locus_tag="LK889_002243"
     CDS             11777..12388
                     /gene="mobC"
                     /locus_tag="LK889_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBK5096447.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            12373..13764
                     /locus_tag="LK889_002244"
     CDS             12373..13764
                     /locus_tag="LK889_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK5096448.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            13980..14294
                     /locus_tag="LK889_002245"
     CDS             13980..14294
                     /locus_tag="LK889_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096449.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            14287..14781
                     /locus_tag="LK889_002246"
     CDS             14287..14781
                     /locus_tag="LK889_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096450.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            14849..15163
                     /locus_tag="LK889_002247"
     CDS             14849..15163
                     /locus_tag="LK889_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096451.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            15262..>15471
                     /locus_tag="LK889_002248"
     CDS             15262..>15471
                     /locus_tag="LK889_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5096452.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         5204 a         2602 c         2782 g         4883 t
ORIGIN      
        1 gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc aaaatcagaa
       61 agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa atcaccgcac
      121 tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt aaaaagttgg
      181 gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt tctttcattg
      241 ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa atttcttcct
      301 tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca tttttgggat
      361 cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg aggaggaaat
      421 aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa tcataagccc
      481 atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg tctatcccaa
      541 ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct ttgagttcct
      601 tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt aaaagctgat
      661 cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct ttcattcgat
      721 aatagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc aaatctttga
      781 aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga atcatccgag
      841 ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga atataaagca
      901 atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct tttatagacc
      961 aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta aactcttgaa
     1021 aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga acaaagtgat
     1081 aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg atggtttcca
     1141 cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt tatgctagtc
     1201 atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa aaagccttca
     1261 atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg ccgacataag
     1321 ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca gtctagtaca
     1381 tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat aatcagaaac
     1441 attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc gccttttcca
     1501 tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt cacctatttc
     1561 tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa gactaaatca
     1621 tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt ggcaacggta
     1681 tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg taagggtaca
     1741 ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa attataggtt
     1801 cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac ttctcggcgt
     1861 aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat accttctgtt
     1921 aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa cacgtaatca
     1981 gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt cacggctttt
     2041 agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac cacctcaata
     2101 tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac cgtagtattc
     2161 cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt tccaaaggtt
     2221 tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt tcattgttag
     2281 ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac gttggttctt
     2341 aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat aatagcccgc
     2401 cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt tctccccggt
     2461 ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa cagttggtgt
     2521 ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta aggtttgaga
     2581 tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat cgtaattgat
     2641 ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt ttttcgcaag
     2701 cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag atttccaaat
     2761 ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag cttcttttga
     2821 gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca catcaccatg
     2881 agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa catggtgttc
     2941 cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg ctactggctt
     3001 atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga caatagtttt
     3061 atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg ttttctcttt
     3121 gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt catctttttc
     3181 ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc agtcttcttt
     3241 gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca gagaagttaa
     3301 ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt cctcaggcaa
     3361 atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca gcttctcgta
     3421 aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg taaagacata
     3481 ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt ctaatggcgt
     3541 aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc cataaggaag
     3601 attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg tggtcgcttc
     3661 atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa aggtcagatc
     3721 attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga aatcaaagcc
     3781 aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat ttttttcgct
     3841 atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat atccttcggg
     3901 cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg caacttggtt
     3961 cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct tcgctaactc
     4021 tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa acaaggtgta
     4081 ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt tgtcttcttt
     4141 cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac tggtaacaag
     4201 ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg tattcacgaa
     4261 cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg cattagcaat
     4321 ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa cgatttcacc
     4381 ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat tgtcattcca
     4441 aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt tttcccgtgc
     4501 gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca tgggggtggt
     4561 atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg taccatgagg
     4621 aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg caggtaaagt
     4681 ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt ctttatcgac
     4741 ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt aggtattagg
     4801 tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg cgacaggtgt
     4861 tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat tggcatttgg
     4921 ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat tttccacaac
     4981 ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg attgccctaa
     5041 tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt caatggcttg
     5101 atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc gttttattga
     5161 gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa tatgagtatc
     5221 tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat cggacatatc
     5281 tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga ctcctggttc
     5341 aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa cagtgacttc
     5401 tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg ctaaatctag
     5461 ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg taggagcaat
     5521 tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata atttcttttt
     5581 ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat aagatgctcg
     5641 cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat agaatggatt
     5701 tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa atatactgcc tttaaattag
     5761 aacacatttt agaacacaaa catttcaaat ttaatcaaat ataataaaat atatttacgg
     5821 gaaaaccttt ataaatcaag gtttttcaaa cttattcaat gatacgaaga aaccaggtct
     5881 taagaaagca cgtaaagctt cacagttttc aaaacgttaa tcgttgttat accaacgatt
     5941 tcagacactt tctcattgcg agaaggtgtc tttttttcgt tctggtaatg agaaaggtaa
     6001 tgacaatatt ataaaccagt gtaagcaatg aatttttcta acgcatttcg ttgagctttt
     6061 tcagtaacat gagaataagt gttaagggta gtttcagcat ttttatgacc caatcgatct
     6121 tgtgtttctt tgatacttgc tccaccttct agtagaagag tagcgtgagt gtgccggaat
     6181 ccatgaatag taacgtgctt aaatttatat ttcttctcta aacgtttcaa tatattattt
     6241 gaatagtccg catgcaaagg ttgattaatg tcaccgtctg catttacata ggtaaagata
     6301 agttgatttt ttgtatttgt atttatgcca aactgcaaaa gctctcgagc ttgttctttt
     6361 ttccagattt ttaacatttc aatagtttgt gcatcaattg gtaatattcg ttcggaagat
     6421 atattttttg tatttttttg aatcctgaca gtttttcttc ttgcattctt ctcttcattt
     6481 cgaatagttg catagctctt tctaacatac attagctgtt cttcaaaata aatgtcagac
     6541 caatgaagag cagctagctc tcctttacgt ataccagtag taatcaaaag ctggaagatt
     6601 aaaatatgtt ttaaaggctc ttctttattg agaatatccc aaaaagcttt tagttcttct
     6661 aatgaaaagt aattttcatt ttctttcttt tcccgctttg gtagatttgt atttgccata
     6721 ggatttcggt gtaatagctc tgcaaaaatg gcatactcaa atatttgaga gctgtaagac
     6781 ttaagctgtt tgatattctt gaattttaaa gcccattcat tgactgcatt ctgacagtga
     6841 acaggagtaa tcttagaaat ggacttattc ccgaattttg gtagtatatg caagcggaaa
     6901 aggtcttttg tacgttgata tgtcgtttct tgtacacgat tcctataaga aggcaaccat
     6961 tcttcataca ctgttataaa caagtcatta gaaggcttat gatagccggt atcttcaatc
     7021 tcgacctttt ttcttgcttc gtgtaacttg gcttcttttt cagttttaaa cccttttttt
     7081 acatagtatt cttgctttcc attttcatct ttacgaacat acaagcggac actatatcgt
     7141 gcaccgcttt ttgttgtgta cttttgtatt gacataacta atctctccaa tctgataact
     7201 tggcgagttg gctgattgaa gaaatagaaa atgagaggag aaaaataatg gatgcaggaa
     7261 ttacagtaat gttagataaa caacaaacga atcaattgaa aaattatgtt tttgaaatga
     7321 caaaagaggc tattgatcaa gcaagaattg ataccggatt agaaagacca tttctaaaag
     7381 gaaaagagat ggctaaatat ttgaatgtat catatacaac ttttttgaaa tttaaaagaa
     7441 tgggattgcc agttatttta ttagaaaaaa tggagttgtt ttcaaaagaa gaatgcaaaa
     7501 aatggatatt gagtcatcaa atttaaaaaa cacctcgttt tttaaatttg atgaagtaga
     7561 aaaaattaaa aagagtgttc ccgtcagttt gcagatcggg ggaacactca ataataaagt
     7621 tctgagaaaa ctttatttga atctattata gcaaataaaa tcagaagcag gtgtgacaat
     7681 gaatgaaaaa gaagttgaaa gcttagttga aaagcaaggc tattctaatt ctgatatata
     7741 tgcagaatta tactatcaat atcctatttt tttaaacgta aatgaaaaat ataagaaaat
     7801 gaaactaagt tctaagttag tttatatgtt actaaaagat agaaatagac tttcattgaa
     7861 aaataacttg tatgatgaga atcaaaaaat ttttttgata tttgaaatca aaaatctcgt
     7921 agaaatttcg ggaatgtcaa aaaatacaat cttatcagca ctagatgaac ttcaaaaagt
     7981 taaacttctt gtgaaaagaa aaaatggctt caataaaagt aaaatgcaaa tgttcccaaa
     8041 tttttattat ttgttaaagc ctgttctgtg caaagatgat gtttataaaa ttaataagat
     8101 aacccatcaa gaaaatagac aaaaaaactt gatagttcaa aatttgaact atggaacaaa
     8161 tcaatattct gttgatagtt caaaatttga actatgcgac acatcaatga aaacaagaga
     8221 caaaaacaat tttgctgata gttcaaaaaa tgaactagaa ttatataagt cttataaaga
     8281 tagtaaagat attaaagaat cacataatga tgatcttctt gaatcacttt caactaaaaa
     8341 taataaatta gatgaagaat tgatcgaaac atttattgaa gaaaatcttc ttaaacaaat
     8401 atggggagaa agtattgtga attgcttaaa actggctagc aatagtgatt atcgtcaatt
     8461 cgacaactgg tacaaaaaat tcaaatatgc gattagatct gctgaaaaag aacaaaaaat
     8521 tcaactgaaa attatctatg aaatttgtaa taacaagtat tttgtagatc atgttagaga
     8581 acagctatca atgactttac gtgacttgat acgaagagca aaaacagatc atcgtatcaa
     8641 acaaaaagac gattatatct ttgcttctct aaaaaataaa gcgttagaac taattgaatt
     8701 gcaaatcagc gaaggaatag ataagcaata agaatagaat tgagtcaaat gaaatggagg
     8761 ggtatctcca cttcatttga gaactttgaa tataggaggg gttattttga ttaaaaatcg
     8821 tttaatagat caagtttggt gtctagatca aaatacagat agtctcgaat taatcgagac
     8881 tatttgcttc aacactatag ttctcgattt aagagctgaa aatgataatt gtgtaactca
     8941 tgtcataatt aatcaaaaaa tggctcagaa gctctctgag agcctcagaa aatgggctaa
     9001 gggaggtaac gatgaaaata attgaaaaaa agattgccaa taaaaaaata aattggttgg
     9061 ttgttaattt tcttgttctg atagctatga ttatttttac aagtaatatt gctcatgcga
     9121 gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat aattctcttt
     9181 atagacctta cctaaaaaag tacaatggag atttccttag tcagttcggg atttggctcg
     9241 gatgggctgt ggtaaaaggc atgtttactg tgacagattc gattcaaaat atgattccag
     9301 atgtgctgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt tatcaatcag
     9361 ccgtaaatac aattgtcatt ggattaatga tcctttcatt gatgtttgtt ggttacaaaa
     9421 tgattactgg aaaaggaaca gtggatttaa aatcggttgg aatgaacgta gtgatgtcta
     9481 tagcattaat acttttaatg ccaaccatga tttctagtgg tattcaattt gctaaaacat
     9541 tttataagga ctctactacg attacaaatt cagatgacgg agtcgcttgg tcattgataa
     9601 aacaaggagt aacagattta gcatatatta ataaaacaga tcagtactca agcattgata
     9661 aaacggagga tcgaaataat ttaacacgaa aaaattttca acagacggat ctcacacaag
     9721 tgttaacgga taaagtgatt gataaattgg aaaaagaaaa tccaattgcg gacaatcttc
     9781 gttatgaatt agttgaaaat tctaataatg aatttgttgc tacaaagttt tctgacaact
     9841 tcttatcaac attttctgat agcttgaaat caggctatta ccgataccaa gcaaatctat
     9901 gggggatttc aattgggttg acagcgttag caatcgccta tgtgttcagc gcgtttgtga
     9961 tcattacagc aatactagaa cttgcattta aacgtgtgtt aggtgttctt gtttttgcaa
    10021 cagatattga gacaggacag cgttcaaaag ttgttctatc agatattcta caatgttact
    10081 taactgttgg gtttcaagga tttgggttat caatgtttgc tatgtttatc aattttctga
    10141 actcaggaca aggtatctca acaaatattt ttataaaaac aattgcttat atctgtgccg
    10201 tttttgtttt aatcaaaggc agtggaacag tcatgagata ctttggtgtt gatattggac
    10261 tcaaagaagg ctatgggcaa cttgcttctg cttttggaat gggcgcaatg ctgtttagaa
    10321 aaggatcaaa tggatttaat cgtgctaaag gaagcggaga aggagaagat cgaaaaccag
    10381 aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa aactggaaga gctttgggat
    10441 atgcccatga acgtggatta tccggtttag cttcagatgg agctacaatg gctagtgaaa
    10501 gagcaacaaa accatttaag agtatgcgtg atatagctaa tgataccaag aataagttta
    10561 aagaaggttt agatgatggt acagtgtccg ctattaataa aaatagcaaa cctatgttag
    10621 ctaaaaataa agaagatgaa actggaaaat atgctgattc cgtgccaaca cgtctttcag
    10681 atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat tatgtctagt tctgaacgta
    10741 tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa tcctgcaagc gcaattcagc
    10801 aaaaagtcca acaagatatt gaagaacgta aaaatgctat gcacggtcaa gctaagtctg
    10861 ctgaagaatt aattaatcag aagcgtcatg aagcgaaata cacgcctgaa gcaatgaatc
    10921 gcgaggaaat aatgttaagg aaacgtgtag aagggcggac aggcgctaat attgacggaa
    10981 aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa ttcaaataca ggattagaaa
    11041 aattagtcaa agaaaatcca caaaattcta caagtagtca tggtggccga tcagtagatg
    11101 tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg acggaatgtt gatgtaagag
    11161 agaatttaca aagttcttca aatgctcaag gtactaagac aattgatgtt cgagaaaatc
    11221 tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt tcgagaaaat gtacaaaatt
    11281 ctacaagtag tcaaggattc aaaacagtag atgttcggga gaacattcaa aaagatagtg
    11341 gagaaatgaa agaaaaaaca caaaaaatca atatcgttga agacagaaag tcagcgaaaa
    11401 aatttgattc aaatcatgaa acagtgatta ttgatagtga aatccgtgaa aatgataagg
    11461 gatcaaaatc aagacgaaga ttcacttatg aagacaatga gttattcaga gatacactta
    11521 atgatcctaa tccacttttc gataatttat taagaaataa aaggggggga ttgtcatgca
    11581 attaattcta aagattagta cgcatgattt cttagaactt gacgaattat gtgaatcagt
    11641 aaagaacgca acaaatgaaa aaatcacggt cactaattat attgaactga tgattcaaga
    11701 agaattaaag atgaaaaatg aaatagagag caatttcaaa tgttgctctc tagaaaaaaa
    11761 tgtgagggga taatcaatgg atgataaaca aaaaatgtac gaaaaaaatc gcaaacatag
    11821 tgaggcctta aattttcgtg tcgataaaaa gacttatctt gaactgacat tgattgcaaa
    11881 acaaagggga atttctagag cagacgtttg ccgtgagcgg ttaaagggac tcaaatatga
    11941 accgccgttg ttagctgaag atgatagggt agttttaacc aaacagttaa atcaaattgg
    12001 taacaatttg aatcaagcgg taaaggcatt aaatatcatt gctaaatatt ttagagaaga
    12061 atctgaagat aaaaaagaaa agagcctaca ttttagtttt gatgctagcc gttacaacat
    12121 ttttaaagat gaacttgcga atagcatagc aaaaaagttg atgattagtt atccagatga
    12181 catttttttg gagtccaatc ggagtgcccc ctcttttttt aaattatcag aagtagaaaa
    12241 agaatatttc gaatattgga tagaaaagca aactagaccg ggtacgaaag aagagtattc
    12301 ccaaaaagtt gatcggaatt taattcactc aattcagatt attgcaagtt taaaagatga
    12361 ggtgaaacaa ctttgggagc tactataaca tcgaaaggac gcaagaaaaa tttatcctcg
    12421 ctattacaat atgcagctaa tccttcaaaa aaagaaaaat gtgttgcagt ctcaggaata
    12481 aattgtagtg atgatagtga ggaagcactg caagaaatga atttagtacg taccaagatg
    12541 aataaatcta aagatatagc acaagcgttt catgttattc attcgtttga taagagtact
    12601 tctaaagagt taagtattga taaaatgcac gaaatagctg tcgaatttgc agagaaagct
    12661 tttccaaatt cgcaaatcat tgttgcaagt cacaatgata aagatcattt tcatagtcat
    12721 cttgtcatta ataatattaa catggaaaca tgtaaaaggt tgcgtattga ccctaaagat
    12781 ttagaactaa tgcgaaaaat taacgatcaa gtcctcaatg aacatggtct tgaatcagtg
    12841 aataatgagt actatgaaaa cttagaaaag agaaccaatc tttattcaaa cgatccttct
    12901 aagatgaaat atggaaaaat taattatcaa gagtttgtgc aaaatgcgat cgaagcaacg
    12961 ttggaaaaat gcgaagtgaa ttcttttgaa gatttttcgg atcatttggc aaaagaatat
    13021 aacattgaag tttatcaatt ttcaaaaaca aataacaaac ttggctatgt tctttacaaa
    13081 caaggtgtaa atttctatga gcgtagaggt gagttaatta ctttaggaag agataaacaa
    13141 tctagaaaag aaaaaagtca gtatgtcgaa cgtactttta gtgcaagaaa gttagggaaa
    13201 agatattcat ttgaatctgt ttctgaacaa cttgaaaaga atttagaaga atcacatata
    13261 aaaaattttt ttaatatgga aagagagcaa gttatgtcgc taattagtgg agtaaattac
    13321 aaaactggtg agttaaatgt accgtttgtg gaattagatt ccatcgcaca aaagcaattt
    13381 agcgaccgtg aaggcgatga aaaattcatt aaatatgatg acttcttgcg agaggtagac
    13441 aagcattttg taaatacaat gtctttagaa gatgatcaag aatacacgat taaacttcat
    13501 acggatgtga atcctcaaac tttacgtagt gcgaaaagta aaacaatttc tattaaaaag
    13561 actgatcaga aagagttatt tctaccggca aagggttttg aaaattgttc tcatacacct
    13621 tctgctaaac agattcacta tcaaatcagt cgcataatga atgacgatgt aaaaatgtat
    13681 cgtgctaaaa aggattatga attcacacaa caaatgattg aaaatcagca aaaaaggcaa
    13741 caaaattttg aaatggaatt ctgaatgggc ttgttagtgg actttccagt tttttgccta
    13801 tgaagagttg cttggaaaga agcagttttt aggacatacg ccagctaccc atctacttgc
    13861 cagtcaatcc gccccgctca cgctaggcta ctttctggca agtcagatgg cgggtaaatc
    13921 tctgagggag caagctccct acagactccc tcccaaaaaa agaaaggtgg aagaaaaaaa
    13981 tgaactatcc atattttaaa gtctcggctt cagaagaaac taaagaaata ttcaacaatt
    14041 tttacaacca aaacaaagga atttttggtt caaaggcaaa tatgtttcgt gttatggttt
    14101 caaatttacc agtgttagct agtccatcaa ataataaatt taatgatcct gaaagcatta
    14161 aatttgaaca aaaaatttca gaactagaat caatgataag taatgaagta attgaaaagc
    14221 tggatgacat tgatcaaaag ttatcgtatt ctctaaaaaa caaatacaaa acggaggaga
    14281 aaaaagatgt ttaaagttta cttcccgttt cagtggctcg ttaaaggaat tcttttagtg
    14341 ctactaggat tgattctttt tggaagttat ctaacaatta aagccagaca ttttccagaa
    14401 gaaaagccaa taaaaattgt aacaaaagat ttaaaaatag gtgaaactta tcaggcagat
    14461 ggggttgaac taacatttaa aaatattgaa gtgaccgaaa aaagaaatga atttgcacaa
    14521 catgatcgtg tattacaagt gacctataca cttataaatg atacagaaaa tccgatacgt
    14581 ccacaacaat ggtcgattga aaataaggat ggtgaagaac tttatgagta tgatatgata
    14641 ttggataatc cagagtatat tgaaagtggc aaagaagaaa cagtaaatta ctattatgaa
    14701 ttaccgattg aaaggcagga ctaccaaatc gtagtacgta gagagaatgg ttacatagtt
    14761 gggttgccat ttactttata agttttattt tgttagtagt ttttaaggta atatcagttt
    14821 tgtgattttt gaaaatggaa ctgggagaat ggctgaggaa aataaagttg ttccgtttga
    14881 aacattagtg ataaataagg aagagtttga gcaacttttt cttgattttt ttattcaata
    14941 tgacatgatt tatgatttta gttcgtcact agatttattt gaattagaag cagaaataat
    15001 agaaaacagt cgagagaaga atggtaaaat cattatttca gtagaaaaat tcaatgaatt
    15061 attctcacaa acatttgaaa tagatccaga ctattatcaa ccacaagaag tttttcgaga
    15121 gtttagaaat aagattattg gttttagtca aactacaaaa tgaaagctta ataaagcaag
    15181 tgttgataga attattcctc aacgcttgct ttattttatg ggcacttcat aatagaggta
    15241 tagagaagag aggtattcat tttggatact attactccaa caaacttcaa agaaaatata
    15301 ggtaaagtgt tgcaagaagt agttcattca aattctgaaa tagaaattac tagggattct
    15361 aaaaatggat tgaacgatgg cgtcattatg atttctaaga gaaaatggga gttgttacaa
    15421 gaagaacttt atttacaacg aacgggtaca tgggcttatg tatctgattt a
//
LOCUS       DAFBQW010000062        14989 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFBQW010000062 DAFBQW010000000
VERSION     DAFBQW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.065"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1206)
                     /locus_tag="LK889_002249"
     CDS             complement(<1..1206)
                     /locus_tag="LK889_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBK5096453.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFI"
     gene            complement(1512..2459)
                     /locus_tag="LK889_002250"
     CDS             complement(1512..2459)
                     /locus_tag="LK889_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBK5096454.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            2649..2918
                     /gene="rpsN"
                     /locus_tag="LK889_002251"
     CDS             2649..2918
                     /gene="rpsN"
                     /locus_tag="LK889_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK5096455.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(3094..3456)
                     /locus_tag="LK889_002252"
     CDS             complement(3094..3456)
                     /locus_tag="LK889_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5096456.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(3467..4588)
                     /locus_tag="LK889_002253"
     CDS             complement(3467..4588)
                     /locus_tag="LK889_002253"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK5096457.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(4604..4954)
                     /locus_tag="LK889_002254"
     CDS             complement(4604..4954)
                     /locus_tag="LK889_002254"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK5096458.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(5084..6595)
                     /locus_tag="LK889_002255"
     CDS             complement(5084..6595)
                     /locus_tag="LK889_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5096459.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(6917..8269)
                     /locus_tag="LK889_002256"
     CDS             complement(6917..8269)
                     /locus_tag="LK889_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK5096460.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(8422..9498)
                     /locus_tag="LK889_002257"
     CDS             complement(8422..9498)
                     /locus_tag="LK889_002257"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK5096461.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWGKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            9694..11439
                     /locus_tag="LK889_002258"
     CDS             9694..11439
                     /locus_tag="LK889_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBK5096462.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(11719..12156)
                     /locus_tag="LK889_002259"
     CDS             complement(11719..12156)
                     /locus_tag="LK889_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK5096463.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(12140..13513)
                     /locus_tag="LK889_002260"
     CDS             complement(12140..13513)
                     /locus_tag="LK889_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096464.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(13835..14323)
                     /locus_tag="LK889_002261"
     CDS             complement(13835..14323)
                     /locus_tag="LK889_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5096465.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(14345..14947)
                     /locus_tag="LK889_002262"
     CDS             complement(14345..14947)
                     /locus_tag="LK889_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5096466.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4234 a         3224 c         2702 g         4829 t
ORIGIN      
        1 aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc
       61 ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat
      121 tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga
      181 ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc
      241 tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc
      301 ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc
      361 ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt
      421 caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg
      481 gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact
      541 ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga
      601 taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat
      661 ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg
      721 cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc
      781 atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc
      841 ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc
      901 ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga
      961 ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc
     1021 actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata atgctttgat
     1081 gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat
     1141 cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga
     1201 ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac
     1261 ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct
     1321 cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga
     1381 aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa
     1441 agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat
     1501 tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca
     1561 actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa
     1621 acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga
     1681 tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga
     1741 tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt
     1801 tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc
     1861 agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt
     1921 tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg
     1981 atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg
     2041 atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct
     2101 gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact
     2161 tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt
     2221 gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg
     2281 aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc
     2341 acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca
     2401 tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg
     2461 gtcaccatct ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaaatg
     2521 tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt
     2581 aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga
     2641 atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg
     2701 aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta
     2761 aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc
     2821 ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata
     2881 aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa
     2941 aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta
     3001 gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt
     3061 atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc
     3121 gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg
     3181 gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac
     3241 ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg
     3301 tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag
     3361 attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga
     3421 aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt
     3481 ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat
     3541 ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg
     3601 ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa
     3661 tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc
     3721 catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt
     3781 ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca
     3841 gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc
     3901 cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg
     3961 ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta
     4021 taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat
     4081 gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt
     4141 ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa
     4201 tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt
     4261 cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag
     4321 gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt
     4381 catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt
     4441 caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg
     4501 gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt
     4561 tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa
     4621 tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag
     4681 gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg
     4741 taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc
     4801 cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag
     4861 aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa
     4921 gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta
     4981 gaacactctc attctaccaa attataacca ttttgccaca aataagtcca caaataacaa
     5041 aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac
     5101 ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat
     5161 tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat
     5221 tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca
     5281 atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt
     5341 ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt
     5401 gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac
     5461 ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt
     5521 tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag
     5581 acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat
     5641 cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag
     5701 caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca
     5761 ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta
     5821 atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag
     5881 gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga
     5941 catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg
     6001 gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa
     6061 gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc
     6121 ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca
     6181 atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac
     6241 gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac
     6301 gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac
     6361 gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca
     6421 ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt
     6481 ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag
     6541 cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa
     6601 tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg
     6661 gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc
     6721 ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc
     6781 gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac
     6841 cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct
     6901 gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca aataagccca
     6961 tgctgttgct gccttttagc ataatactat ctgttgctgc aagattttgt ttgatagcat
     7021 caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt
     7081 tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct
     7141 caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca
     7201 gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta
     7261 ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat
     7321 ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc
     7381 gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg
     7441 ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg
     7501 tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg
     7561 ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca
     7621 caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc
     7681 cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt
     7741 ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca
     7801 aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat
     7861 tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta
     7921 gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt
     7981 tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa
     8041 ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt
     8101 ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata
     8161 aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag
     8221 agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc
     8281 ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt
     8341 ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc
     8401 cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta
     8461 tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt
     8521 ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt
     8581 caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc
     8641 taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac
     8701 ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa
     8761 tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac
     8821 agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg
     8881 ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac
     8941 actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg
     9001 atcaaatact tttttaggat tttctttcca ttgattctta agtactggta cataaggaac
     9061 ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc
     9121 actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat
     9181 cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt
     9241 gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc
     9301 ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgcc
     9361 ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc
     9421 atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata
     9481 tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc
     9541 tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc
     9601 ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat
     9661 aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc
     9721 ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa
     9781 aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata
     9841 ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa
     9901 acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca
     9961 gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct
    10021 gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag
    10081 atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg
    10141 atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa
    10201 gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa
    10261 caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct
    10321 ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta
    10381 atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa
    10441 caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt
    10501 ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag
    10561 acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt
    10621 cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt
    10681 ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg
    10741 ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa
    10801 ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat
    10861 gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt
    10921 catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca
    10981 ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct
    11041 tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga
    11101 ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta
    11161 ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa
    11221 ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca
    11281 ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt
    11341 tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc
    11401 gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga
    11461 atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa
    11521 ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag
    11581 ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt
    11641 ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc
    11701 ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg
    11761 atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc
    11821 actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga
    11881 gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt
    11941 gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc
    12001 gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac
    12061 actggtcacg ataaccacgt caagaccata ggattctcct aattttatga catcttcttt
    12121 tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat
    12181 gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa
    12241 gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga
    12301 ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga
    12361 tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag
    12421 ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac
    12481 taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac
    12541 tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc
    12601 cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt
    12661 tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta
    12721 gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct
    12781 ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat
    12841 caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga
    12901 atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat
    12961 ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat
    13021 aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga
    13081 gaaatagcca gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg
    13141 tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta
    13201 gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg
    13261 aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc
    13321 gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca
    13381 aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa
    13441 taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga
    13501 gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt
    13561 tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag
    13621 aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc
    13681 cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc
    13741 aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc
    13801 gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat
    13861 aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc
    13921 cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct
    13981 ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc
    14041 cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt ccatttatct
    14101 cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac
    14161 cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca
    14221 cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt atgactgcca
    14281 tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt
    14341 tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac
    14401 ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg
    14461 tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa
    14521 ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact
    14581 tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc
    14641 cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa
    14701 acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga
    14761 tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt
    14821 gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga
    14881 aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt
    14941 tagacatcta tattccccac atcttttttc atttattaaa actgcctcc
//
LOCUS       DAFBQW010000063        14837 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFBQW010000063 DAFBQW010000000
VERSION     DAFBQW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.066"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            417..1031
                     /locus_tag="LK889_002263"
     CDS             417..1031
                     /locus_tag="LK889_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5096467.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1804..3543
                     /gene="yfcC"
                     /locus_tag="LK889_002264"
     CDS             1804..3543
                     /gene="yfcC"
                     /locus_tag="LK889_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK5096468.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3700..4884
                     /gene="iadA"
                     /locus_tag="LK889_002265"
     CDS             3700..4884
                     /gene="iadA"
                     /locus_tag="LK889_002265"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK5096469.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4995..5198
                     /locus_tag="LK889_002266"
     CDS             4995..5198
                     /locus_tag="LK889_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK5096470.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5299..5961)
                     /locus_tag="LK889_002267"
     CDS             complement(5299..5961)
                     /locus_tag="LK889_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096471.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5974..7476)
                     /locus_tag="LK889_002268"
     CDS             complement(5974..7476)
                     /locus_tag="LK889_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5096472.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8024..9703
                     /locus_tag="LK889_002269"
     CDS             8024..9703
                     /locus_tag="LK889_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK5096473.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9830..10309
                     /locus_tag="LK889_002270"
     CDS             9830..10309
                     /locus_tag="LK889_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBK5096474.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10528..11208
                     /locus_tag="LK889_002271"
     CDS             10528..11208
                     /locus_tag="LK889_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK5096475.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11216..12547
                     /locus_tag="LK889_002272"
     CDS             11216..12547
                     /locus_tag="LK889_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5096476.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12562..13524
                     /locus_tag="LK889_002273"
     CDS             12562..13524
                     /locus_tag="LK889_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBK5096477.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQEDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13635..13973
                     /locus_tag="LK889_002274"
     CDS             13635..13973
                     /locus_tag="LK889_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBK5096478.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14052..14759
                     /locus_tag="LK889_002275"
     CDS             14052..14759
                     /locus_tag="LK889_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBK5096479.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4761 a         2578 c         3193 g         4305 t
ORIGIN      
        1 ttttttgtat tttcttgctt gcacttggat aagagattac ttacaataaa ttcagataga
       61 aacagctgaa taaaaaaagg ctccctgacc cttagttggc gctaagaatc agggaatgac
      121 cagtcacaca catacaccct gaccgatcat tttgccatag tcaatgcagt gcatcgacct
      181 aatttattgt acctaatcta gggtgaaaat tctaccccta tgtagatatt ttccatggtc
      241 tgcacgttgt tttatttgct atgtcaaggt attgggcgta ttcgttcaat gccttttttg
      301 tgtttgaaac agcaaagacc acatgtgtta ctgtctaact actgtttttt tatgtacttc
      361 cttagattag gcagctgttt ctattcatgt caagtatagc taggcgggag agataaatga
      421 gtttatgtta ttttgactat tatcaccaat tggaagagaa attaaggata gaagagccgc
      481 tgatgcttca agctggcact tggatcgcga aacaaattaa tcaaggtggt agattgcaga
      541 tatatgctag ccgcacacta agtggtgtag catttgaatt ttgggatcaa accccggatc
      601 ttgtaccaag tatattgatt gaaaatccag ccggaggtgt ctatgaaacg ctagaaggtg
      661 caggacaggc aattattgat cagctttctg tcaaatcaga agacattttt cttcttcttt
      721 cgaatgaagg aagaaacccg gcaatcatcg aactagctca gtggatcaaa gaaaatggtc
      781 atccattaat cattgttact ggatttgatt tgtcacgaag tatcaaatcg caacatagca
      841 gtggtttgcg attatttgaa tttgctgatt tggttttgga caaccacgcc aatttacatg
      901 atgcagtctt gactttgcca aatctagatc cagcgatctg cggaacagcc tctcttgcaa
      961 cgatcttttt gctgcagcaa gttttgtatt ttacagcaaa acagctagtc caggacacaa
     1021 gtggaaaata gttatccaca tgttgacaaa gttccttaaa aaaataatgt tccacataaa
     1081 aatggagcgt ttgaaaaaaa gaaaacgatt attaatgagg attaacaacg actacttttt
     1141 ttgttttagt tattttaaga tggatcatct ttgattttaa gcttgttttg tttaaagttt
     1201 gttattttta taagttgttt cacagttatg agaaatgagt tataagaata tgtagtgtca
     1261 gaaaaataaa aagtagtgga aaaagagaca agaaagaatc catatggatt ctttcttgtc
     1321 tcttaataca cgttgatgaa agagtcttcc ccaaaactct ttacaaagta attatagtac
     1381 atttgttatc attattgcaa tgaagtttac ctgatataac aaaattgtcc aacaggatac
     1441 ctttttctaa tagaaataaa atacacaaaa attgaaggat agataatgga tatttataag
     1501 tgtttattat tttttttgca atgaaattca atttttcaat gattgtaaaa tgaaaaaaac
     1561 aggacgagat tttctctaat ctaaaaataa aaaaagaatt ttaaggaaaa acagagaaat
     1621 aaaatactta atataaatgg aaagagaaaa aaataatcat acataggaga aaaaaaacaa
     1681 aatagaaaaa agtgattttc taataaatta ttcatgtgga aatgtcattt ttactgtata
     1741 attcatttga ttatccattt tttattcatc aaaattactg aaaaaagaaa ggaagaaata
     1801 aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca
     1861 ccccatacgt atgtcattat ttttggtgta gtcatctttg catggatact gacttttgtc
     1921 gttccagcag gaaagttcag cacgcaagat attgaataca aagatgcaaa tggcgaaaca
     1981 agtactcgta ctgttttacg tcaagattcc ttccgttatg cttacgagct ggataagtcg
     2041 tatgtgtttg atcaattaga agaactgcaa gatcatccag cagaacgtga aaaactggat
     2101 gttccggaaa aaggattgga aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa
     2161 aaactagatg aaatctcctt gacagatgat gtgctttacg atcagtacgg cgaaaatatc
     2221 tatgatactt ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga tgattttggc
     2281 ggctttggtt tcttaaattt tgtttttgaa ggactagtat ctggagataa gtatggatct
     2341 gctgtaggga tcgctgcatt gatccttgtt gttggaggtg cttttggtat tattatgcgt
     2401 accggagcga tcgacgcagg tatctatgcg tttatcagta aaactaaggg actggagcgt
     2461 ttagctttac cgctattatt ctttgctttt tcattcggtg gcgctacatt cgggatggca
     2521 gaagaggtaa ttccattctc aatggtgatg gttccatttg tcattgcgtt aggatatgac
     2581 tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc gacctcttgg
     2641 atgagcccgt ttagcgtagc ggtggctcaa gggattgctg gaatccccgt gttgtctggt
     2701 gcgacattcc gattgattat gtgggtagtt gttactgcat tagccgctgg ttatatgatg
     2761 atatatgcag aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa atcagatgat
     2821 tatttccgtt ctcatatcaa aaagacatct gatgaaaata aaaaattcat gcttgggcat
     2881 aaattgatct tattagagat gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa
     2941 aaaggctatt acattccaga aatcgcttct caattctttg tgatgggatt agttgctgga
     3001 atcattgctg ttcttttcaa attagatgga atgaaagtca atgacatcgc ttcttctttc
     3061 caatctggtg cagctgattt agctggtaca gcaatcgtag taggtatggc caaagggatt
     3121 cttcttgtct tgggaggatc tgatgcatct gttccttcag ctttgaatac gattttgcat
     3181 gggatcggga cggcgttgac cggtgttcca gctgtcattg gtgcttgggc gatgtacatc
     3241 ttccaaagtt tgttcaactt ggttgttaca tcaaattcag gacaagcagc tttgactatg
     3301 ccaatcatgg ctccactggc agatttagta ggtgtatcca gacaagtagc agttcttgct
     3361 tatcagcttg gttcaggttt tatggacgct tttacacccg tatcagccag cttgattggt
     3421 gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa gattccaaat caagatgcaa
     3481 ggattcctgt ttgttttagg aactattttc atcatgattg ccattgcaat aggtctgcag
     3541 tagtactgga gacctgtaaa taaaaaagta atccaaacag tagataagga ctccaagaac
     3601 cgtcatttta cggtaaaatc aaaggagtaa agatcctatc gtttggatta ttttttaaga
     3661 tcctatcatc agttatactt aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt
     3721 gaagtgtttg caccagatta cttaggaaaa atggatgtac tgattgccgg tgggaagatt
     3781 ttggctgtag aagaacagct agaaggcggc tacgaaggag tggaagtaga agaactttca
     3841 ggtgagggaa aagtactaac cccgggtttc attgactgtc atttccatat tttaggtgga
     3901 ggaggagaag gcggttatca aaatcgaacg ccagaagtca cattgagcca attgactaca
     3961 gctggtgtaa cgacagttgt cggttgtttg ggaacagacg gagaaggtag agatatgact
     4021 gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa tctccactta tgtatacgaa
     4081 ggttcgtacc gattgccggt aaaaacagta acagattcta tcatcaaaga ctttttgaca
     4141 attgataaaa ttatcggaat cggagaaatc gcagtatccg atcaccgttc ttctcagcca
     4201 agttttgaag agttcgctcg tgcagtagca gatgctcgtg taggaggcat gttatccgga
     4261 aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga gctattgaca
     4321 cgaatcgtag aagaaacaga aattccgatc acacaatttt tgcctactca tgcaaatcgc
     4381 acaccagaat tatttgaagc ttgcgtggca tttgccaaac gtggtggcac gattgacttc
     4441 actgctagcg aagatcctga tttttgggag aagacagatg gtgaagttcg cttcagcaaa
     4501 gcactgaaac gattgatcga ggaagatgtt tctttagata atttcacgat gacctcagat
     4561 ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt tcctagggtt aggagtagga
     4621 agtgccaagg cattgctagt agggatcaaa gaagctgtgc aaaaagaaag tatccctttg
     4681 gaaatcgcct tacgagctat aaccagtaat ccagcacgta tattgaaatt ggataaaaaa
     4741 ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct tggataaaga aacgcttgat
     4801 attgatacag ttatcgctaa aggagaaata atggtccaag aaaaagaagt caaagtatgg
     4861 gggactttcg aaaaaagttt ttaatcaaca gctctaacta tcttttttag aaaacgagcg
     4921 ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg tagatcaata aacaaactaa
     4981 gaggaggaag cacaatggca gatttaaaag gacgttctaa agatatgaaa gataaagtag
     5041 tcggtgaagg aaaagaagca tacggcaaag caacagacga caagagcaaa gaaacagaag
     5101 ggaaagcaca atcactaggt gctgacatca aagaaaaagc tcgaaattta ggcgacgaag
     5161 tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa accacttatt actaattgaa
     5221 tcaagcaaat aaaaaatccg aaccatatct gcgagacaga tatggttcgg attttttatt
     5281 tgatcattat cctgtttttt aaacttcgat tttttgaaat tttttatttt ttagttcaaa
     5341 gacttcatca cagctttttg ctacttcaat ctcatgagtc acgatgataa tacagcgatt
     5401 ttgttcatgg gcgatttctt ggaaaagttt gacgatatct tgtgaagttg tttcatctag
     5461 atttccagta ggctcatccg ctacgattag ttgatgttca cagcagatag cacgaacgat
     5521 agcgactcgc tgctgctgac ctcccgaaag tttggtcacc tgtttttttg ctaatatttc
     5581 atcaattcct acacgagcca gtcgatctaa agcaacttgt cgataattct cttgcttggt
     5641 cttagtgatt tccattgctg ctatgacgtt gtctagagcc gataaataag gaagcaagtt
     5701 gtaagcttgg aaaataatcg agacatcatt tttacgataa cttcttaacc cgattttgtt
     5761 cagtgaacga tcttgatagt atacctttcc ttcttttggt gcatctagtc cagaaataag
     5821 cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa atagcgtaca tttttccagc
     5881 ttcgaaattc agttctacat ctttataaag cgcatcttgc ggatcttgat accaaaatcc
     5941 caattgttct gtccgtaaca tcaaattcct cctttagttt aataggattt ttcttgggtt
     6001 taatcttaat atccctatag aagctaaaaa gacagaaaac agactgattc caaatgctac
     6061 tccggctaat agtcccaatt gtgcaggttt gacattgatt tccaattcgc tgacttgatc
     6121 agaaacagca aatggattat tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc
     6181 ttgtctgtta cctggcattt gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc
     6241 tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg acgacgtttc cgctaagcga
     6301 agcaactcct aatgcaagaa tcatacaaat agccacttcg gtaaacaatt ggagaatgat
     6361 tttacttctg gattctccta aagatagcaa gacgcctaac tcatgccgac gctcccgaat
     6421 ggtaatcata atgatcagag taagtatgat aataccggct acagccacta gtaaaacaac
     6481 atttttagaa aaacttgcca cgttgtttaa aggttccaac attgactgat acatggaatc
     6541 atttgattgt aagctaaatg tatctgtgtc gatcagcttc tcagcttctt tgagaaactc
     6601 atccatttta tctggatcag atagagtgta aatagcagaa tctatcgtgt tatcgtcaga
     6661 tgtcccattt agttcatttg caaaagtgta agaagtgtaa agtgtattcg acggattcat
     6721 gaagttgaat ttcataccca tactgccgct tgtttcgctg gattcataaa ttcctttgat
     6781 cgtcatttca taagtggtat cttcatcttc agtactcgtg attgtgaaag tatctccgac
     6841 tgccaggtca tttgtttctg ctagagtaga atcaatgact acattgtttg tgcctttgtc
     6901 gctgtcatct attccttcac catccgtgat ttttgctgtt ccatcagaaa aattactatt
     6961 ttgtgcagtt gctgacacac cactgatttg aaagtctgct tgagtcattt gcggattccc
     7021 gcccattcct ccagcaggtt caccgcctgc atcagtagtt tctgaactgt cttcacttga
     7081 atcagaacta gtgatggcag ttattccatc ttttgccata gctgaggcgg aactttcaaa
     7141 ggaataactc tttacattat ctaattcagc tattttttcc gcgtccgtta aggaaactgg
     7201 tgtaagagaa aaactgcctg gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt
     7261 ttgttcctgt tttttaaatg aagcttctcg attggcactt aaagtcactg ttgctccaac
     7321 ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag
     7381 cacaaaaata agaatggctg tgaacacggc aaacaataac aatgaacgtc cccattttac
     7441 ttttgtactt ttccatgcac gtttcataaa attcatattt tgcatccctc ctagtagatg
     7501 catttgataa tagacaacct atcttaaagc tagcttaaat cttatgctgt ttgcacacca
     7561 tcaacatgag gttctgtata tataaaaagt cactatagtt attgcaaaaa atgtggtaaa
     7621 atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa agaggaatag caaaagaaat
     7681 cctagatgct acagagggat ttttacgtgc atagttgttc taacaattgc tgatactagc
     7741 gatttagttt atttgttgta taataatggg gaacagctga atacttatat aagtattctg
     7801 gctatagcgt ttcgcaaaag atccattttg ttaatgaagt atatccaaaa taaaatgccc
     7861 atccaactgt cagaacgcgg atgtgctggt aattttcctg ctgattctcc ataagttgag
     7921 aaatagtgat tttgcatatg cttcattgga atggctcata tatttagata aagaagaaaa
     7981 tcgacatctt taaacttgga tgtcataaga agaggtgaag tttgtgagta ccattaaaat
     8041 tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga
     8101 ggatattttt attttagatt gtgggctaaa atatcctgaa aatgagttgt tggggatcga
     8161 cgtcgtaatc cctgatttta cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt
     8221 tttaacacat ggacatgcag atgcgatcgg ggcacttcct tacttgttgt caaaaatttc
     8281 tgtaccagta ttcgggacag agttgacgat cgagttagca aaattgaatg tcagtcgtaa
     8341 tgaagcagcc aaaggattta aggatttcca tgtggtagat gagcatacag aaatagactt
     8401 tggtgaaaca gtagtgagct ttttccgtac cactcatacg ataccagatt cagtggggat
     8461 caatctgaaa acaaaagaag gtagtatcgt ttatacggga gattttaaat ttgaccaaac
     8521 ggctatccca atgtatcaaa cagattatgc acgattggcg gaaatcggca aagaaggagt
     8581 cttagcctta ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag cttctgaagc
     8641 ccaaatagca gatgaagtat ttgatacgat caaatattgg gaagggcgta tcatcgtcgc
     8701 ttgtgtagca agtaatttgc aacgagtgca acaagtattg gatgcagcag aaaaagcaca
     8761 acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt attcgcacag cgatgcgttt
     8821 agaaaaactt caattaccaa gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta
     8881 tgatccagaa gaactgatca ttttagaaac tggccgaatg ggtgaaccca tcaagtctct
     8941 gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag atttggttta
     9001 tatcactact acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat
     9061 ttatcgagct ggtggaacag tgaagttgat ctctgagaat atgcgcgttt ctggccatgc
     9121 caatcctaat gacttgcaat taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt
     9181 tcaaggagag tatcgtgaat tagctgctca tgcagatttg gctcatgcag tagggatgcc
     9241 atacaaaaat atctatatta ctggacgcgg agacgtgctg gaatataaga acaagcgtat
     9301 gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt gatggattag gcataggtga
     9361 tatcggaaac attgttttac gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc
     9421 ggttgtcact atcagtcggc gagaaaagaa aatcatttca aaacctcaaa ttacttcacg
     9481 gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca atatgattga
     9541 aacaatcgtt gagaaacatt tagaaagcaa tgattttgag tggagcaaat taaagcaaga
     9601 catccgagat caattaggcc gttacttatt tgaacaaaca aaacgccgcc cagtgatctt
     9661 gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg
     9721 aacctctcta tagagtgctc gtcttgctct ttttttgcga aaaacaaata atttttgaat
     9781 acagcatccc tcgctttata ataaacgata gaataaaggg agtgaatcaa tgatgagtca
     9841 aacaaaagtc gaacgttatt tagaagaaca aaagatcagc tatcaaccac ttgattttag
     9901 tcatgtggta gaagaatctt tcacagaaga actacaaaaa agagggattg atccagcttt
     9961 gatatgtaaa actctcgtag tgaaaggaaa caaaactggg gtgatcatcg cattgattcc
    10021 tttgaacgat cacttagatt acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg
    10081 ttttcctgga atggattttg tattaatgca tacaggatat ccccacggag caaacacccc
    10141 tattggaata aaactcgcac atcctgatta ttgtttttta gcagacagtt ctttaaaaga
    10201 aaaagaagaa gtaattgtgt caagcggtga aatcgggaga agccttcgga taaaagtagc
    10261 cgatttagaa aagatcgtcc atcctattta cgctgattta attagttaag ggacgagaag
    10321 agaagctaag cagagcagag acctcaaact tgagattata tctgcttggt acttggtgca
    10381 tcctgtatta tctgttacaa ttgctttgta catactgtac tatcaatcca atgaaaatat
    10441 atttagcagc tatatttttt cattgatgtg caagtgatga aatcgttttt aataaaaaag
    10501 atacagagaa aacgcgaggt gtaaaatatg aaaattacgt atcatggaca ttcctgttta
    10561 acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc cgtttattac aggaaatcca
    10621 ttgacagact taactgcaga agaagtaaag acagattggt tgctgattac tcatggtcat
    10681 agtgaccaca taggagatat gattcctatt gcaaaagaaa atcaagccac aattatcagt
    10741 attgttgaga ttgctgatta tgcgaacagc agaggagtag attcattcgg tatgaatatc
    10801 ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca gcatagttca
    10861 agctatgaag tagacggtat cgttcagtat atgggcgagc ctagcggaat tattattcaa
    10921 gcagaaggga aaacgattta tcatgcaggg gatactgctt atttcagtga tctaggattg
    10981 cttgcagaag aattcgatat tgatgtcgct ttcttgccga ttggcgataa ctatacgatg
    11041 gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc
    11101 cattacaata ctttccctgt cattgaacaa gatcccgaat cttttgtaga gttactggaa
    11161 gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc
    11221 gacaaaacat gatttgattt tagaatatat tgaaagttta cctgttggca atcgaatttc
    11281 tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa
    11341 agatgcagaa aatgtcggac tagtttcaac aattcaacga gtagggacca tccgtatcga
    11401 acgcaaatta aaaaaacata ttgaacgttt gacatttggc gaggtcgtca ggatcattga
    11461 aggggacgtt ttaggcgggg cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg
    11521 cgcgatgact aaacatgcca tggaacgcta tattacgcca ggatcactaa tgatcgtcgg
    11581 caaccgggcg gaagtccaaa aattagcatt ggaagatggc gccgcagtat tgatcacagg
    11641 aggattcaat acgactgctg agattgcaaa gctagctgat gatcttgaaa tgccgatctt
    11701 acgcacctct tatgatacat ttacagtcgc aaccatgatc aaccgagcgt taagcgacca
    11761 attgatcaaa aaagatatca tgcttgtcag tgatatctat atgccagctg aaaagactca
    11821 ttatttgcag cagacagata cgatttctga ttatcagcgt ttgtcagaag aaacgcagca
    11881 ttcgcgtttt ccagtcgtca accgccatca tcgtttgatg gggattgtta cggcaaaaga
    11941 tgttttaggg aaatcgccaa accagatcat cgatcgggtg atgacgaaag aaccaatcag
    12001 tgtaaagaaa acaatgagta ttgcctctgt tagccatcaa atgatctggg atgggctaga
    12061 agtcatgccg gtcgtttccg atgacttgac attggaaggc ttgattaccc ggcaagatgt
    12121 aatgaaagcg atgcagcttg tccagcgaca accgcagatt gcagatacga tttcggatca
    12181 gatatctgga aatatccata cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc
    12241 taagttcaaa ttcgtagtag caccacaaat ggtcaatggc gttggaacca tttcttttgg
    12301 cgtacttagc gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg
    12361 aaatatcttg attgaacaag tgaacctaca ttatttgcgg ctgatccaat tggaaagcga
    12421 attagacatt cgtccacgtg tattggaaat cggcagacgt tccgctaaac tggatatcga
    12481 agtgtttatt gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg
    12541 ttcttagaaa gcaggagtga aatgacagta cagcaagaaa tagtagatac aataaaaaaa
    12601 tacgataaaa tcattatcca caggcatttg cgccctgatc ctgatgcatt aggatcacaa
    12661 gtaggattag ccgaactatt acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt
    12721 gaaacgattg aagggcttcg ctttttagca gaaatgcaag aagttgaaga tgtcttctac
    12781 caaggagcgc tagtcatcgt gactgataca gctaattcac ccagaatcag tgatcaaaga
    12841 tatcagttgg cagaaaaact gatcaaaatc gaccatcatc ccaatgatga cccttatgga
    12901 gatcttgtgt gggtaaatac aaaagcaagt agctgtagtg agatgattgc agaatttgct
    12961 ttgatgtttc ctgaatcttt aaagatgaac gttaaggctg cacggttact ttatgcagga
    13021 atcgtcggag atacagggcg ctttttgtat ccctccacga cgtcccgtac attagagatt
    13081 gcttcgatat tgagaaatta tccatttgat gcaagtgctt tgaacagaga gattgaacaa
    13141 atgccgatga aagtcgctaa actttccggt tatctttatc aaaatatcca ggaggatgaa
    13201 aatggtgcag gaagaataat ccttccgcaa gaaattcttg ataaatatgg aatcgatgat
    13261 tcagagacag cttcgatcgt ttcacttcct ggagtgattg acgaagtgct ggcttggggg
    13321 atttttgttc agcagccttc agggtattac cgcgtacgtt tacgttctaa ggggccgatc
    13381 atcaatgaat tggctaaacg ccatcatgga ggaggccatc cattagcaag tggtgcgaat
    13441 gcaaaagatt tagcagaagt agaggaaatc tataaagaga tccaagatat atgccaatcg
    13501 tataagagca gaaaaacaga atgatacttc gttcgaaaac gtgccttttg tagtaaaaat
    13561 cacaaatgta tcttttcaag atcttctttt tccgttacaa tagtgctcac taaagatttt
    13621 aaaggagaaa gaagatgaca ttaccaaatt gcccagaatg tggctcagag tacacatatg
    13681 aagatcgagg cttgttgatt tgcccagaat gcgggaacga atggaacaaa gaagaacaag
    13741 aaactgtcat agaagacgga ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg
    13801 gcgatagcgt gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt gccattaaac
    13861 aaggaacaaa agtcaagaac atccgtttag tagaaggcga tcataatatc gattgcaagg
    13921 tagaaggatt tggaccgatg aaactgaaat cggaatttgt gaaaaagaat taaagaatat
    13981 tcaaggtctt ttcacattct tttcctacta ctaatgataa aataaagtaa acgaaaagga
    14041 gtgaaacgaa aatgtatggt tatggaatgt atggtttttt agatccgaca tttcttttgg
    14101 tcattcttgg attagttatt tccggaattg cgtcagcgta cgtcaatagt actttccgaa
    14161 aatacgatca agtgagaagc aaaaacaatg tgactggtac acaagcagca caatatatat
    14221 tacaaagtca acagataaat aatgtcggtg ttcaacaaat tgccggagat ctcacagata
    14281 actacaattc aggtaataag atgttgagtt tgtctcaagc aactgcacag tctacttcag
    14341 ttgctgcgat aggtgtagca gctcatgaat gcggacatgc ggtacaagat gcttcgggtt
    14401 atgtcccatt gaagattcgt gcagcacttg ttccagcagc taactttggt tcgatgattt
    14461 cgttcccctt gattatggta ggtgtattgc ttagctggaa ctctacgtta atcaatatcg
    14521 ggattttcgc tttttcttta gcattacttt tccaattggt gacattacca gtcgaattta
    14581 acgcgtctcg gcgagcaatc caaattttga gtgaaggcgg actactgacg gaagaagaag
    14641 taccgatggc aagacacgta ttatttgcag cagcactgac ttatgttgca gcagcgcttt
    14701 cgacattcct gcagctgttg cgcttgatct tattgttcgg cggcaaccga agagattaaa
    14761 ataaagaagt tgtgaaatca gcgctttgat ctgagctaat accgagagtt agctgattga
    14821 ataccattta tttggaa
//
LOCUS       DAFBQW010000064        14496 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFBQW010000064 DAFBQW010000000
VERSION     DAFBQW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.067"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            136..456
                     /locus_tag="LK889_002276"
     CDS             136..456
                     /locus_tag="LK889_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096480.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            908..1108
                     /locus_tag="LK889_002277"
     CDS             908..1108
                     /locus_tag="LK889_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5096481.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1255..4044
                     /locus_tag="LK889_002278"
     CDS             1255..4044
                     /locus_tag="LK889_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK5096482.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4091..4618
                     /locus_tag="LK889_002279"
     CDS             4091..4618
                     /locus_tag="LK889_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBK5096483.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4639..5829
                     /locus_tag="LK889_002280"
     CDS             4639..5829
                     /locus_tag="LK889_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5096484.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5869..7167
                     /gene="asnS"
                     /locus_tag="LK889_002281"
     CDS             5869..7167
                     /gene="asnS"
                     /locus_tag="LK889_002281"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK5096485.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8343..9017
                     /locus_tag="LK889_002282"
     CDS             8343..9017
                     /locus_tag="LK889_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5096486.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDNIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9014..9355
                     /locus_tag="LK889_002283"
     CDS             9014..9355
                     /locus_tag="LK889_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096487.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9385..9744
                     /locus_tag="LK889_002284"
     CDS             9385..9744
                     /locus_tag="LK889_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK5096488.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10269..12353
                     /gene="cadA"
                     /locus_tag="LK889_002285"
     CDS             10269..12353
                     /gene="cadA"
                     /locus_tag="LK889_002285"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK5096489.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            12644..14110
                     /locus_tag="LK889_002286"
     CDS             12644..14110
                     /locus_tag="LK889_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5096490.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4799 a         2510 c         3103 g         4084 t
ORIGIN      
        1 agaggttgtg acgtggtcac gacctctttt tgacgattat gatacaaaaa acatcattta
       61 tgaacaaaat gtgaataacg ggcgttcttg agatatgatt ttaattggga aaaacttatc
      121 gataaggagg tctttatgaa aaaaagactg ttatggtgtt tgctgtttct atccctaagt
      181 ttaacaagtc agccagtttt tgctgaagaa acacagcggt ctcctattac aggcacacgt
      241 ctgtacaaaa atgtttatgt acagacaaca gatcttgatt ctgcgattcc tgaaacagaa
      301 gaacggtctt tgcagccaag atttggttac ttttggtact gtccgacctg tgattatgaa
      361 agttgctggc atttcttaca ttctacagct gcagcttttg cttttactca tactcagaaa
      421 tatgatggac atcatcctgt tatttatgag gtatagatat gagcaaaaaa gaaaaactaa
      481 tctcgttgat cgtattggtt tgccttgttc ttctggtaac tgtcataggc tatcagaaat
      541 accatgagaa catcagacct actaaagaat cttccccagc ctcgaccgtt gaaacagaac
      601 cgataagtgg tcagaaagaa taattttttt agtttaaaga agaacatcag agcaaaaaaa
      661 caccaactga acaatatgtc gtttatttta aaaaaagttc ggtttttcac tttatgtaat
      721 cgctgagaaa acttttttta ataaaaaaag tcgaaaaact ctagcttttt tgtaaacggt
      781 atgatatact ataggagtag tttttgttcg gtaatctgtg gtagcttgtt tcaaataaac
      841 atcttccaga tatacaccat tcaagtaatt gcaaaatatg tgttgataca caaggaggat
      901 ttttattatg gaaacaggta cagtaaaatg gtttaactca gaaaaaggat ttggattcat
      961 cactgcagaa aacggaaacg atgtattcgt tcatttctca gctatccaag gcgacggatt
     1021 caaaacttta gaagaaggcc aagcagtaac tttcgatgtt gaagaaggcc aacgcggacc
     1081 tcaagcaaca aacgttacta aagcttaatt aacctatact aaaaaataaa cgcccagaca
     1141 tttgcgtgtc tgggcgtttt tgtctatttt ctttaaaaag cgatgagagt atttttctgc
     1201 actttgtagt ataatttcaa aagaacatta gaggcaggag aagaggaaga tattttgaaa
     1261 ccaagaactt atgcagtcgt tgatatcgaa acgactggga cgaatccaaa agaagatcgg
     1321 atcatccaat ttggctgcgt catgattgaa tcaggtcgga tcacttcacg tttttcgatc
     1381 gatattcatc cagggcggaa gatttcaaaa caaatccagc atttaacagg gatcacgaat
     1441 caacgcgtcc aaaaagcgcc ttatttcgaa gatgtcgccc aaacgattta caatcttttg
     1501 gcagacacca tatttgtcgc gcataatatt tattttgatt ataatttttt gaaccatgaa
     1561 ctcatgcgat gcggacttcc tagtttgaag ataccaggca tcgatacagt agaacttgca
     1621 caaattttct tgccgacgga accaagcttt cgcttagctg atttgtcaga aagccttgga
     1681 tttattcatg agaatcctca tcaggcagat agtgatgccg aagtgacggg tcaacttctt
     1741 ttgctgatcg aagaacggat gagaaaattg ccaatcatca cgttagagca gattgcgcga
     1801 ttaagtagac acacaggtat ggatacgagt cgtttcattt atcatgtgat tgaagaaatg
     1861 aaagaaaaac cagagccact tgatccctca ttagaaatcg tcgatgggct agctttgcag
     1921 aaaaaagaag tggaactatt cacatccgtc cattatggcg agcggacata tccaagaaaa
     1981 aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga acagaacaga
     2041 ctgatgaatg cggtctacga ccatttcaca aaagatgaat caaaagattt gatgatcgaa
     2101 gcagctacag gaatgggtaa aacaatcggt tatctgctgc cattgagtta tttggctaca
     2161 cctgaaaaac ccgctgtcat cagtaccgtt tcgctagttt tgcagcagca gatcatagaa
     2221 aaagacattc cattgctgaa tcaattgcta gatcagccca tacaacctgt aattatcaaa
     2281 agctatcgcc actatattga tttgcagcgt tttaaaggca cacttgtgga accgccagaa
     2341 caaaaacaat atgctttgta tcaaatggcg gtcttagttt ggctgacgca aacgaagact
     2401 ggtgatttgg atgaattaca tttaacaaat ttgaaccatt cattctttgc agatatcgcg
     2461 cacagaggta ttggattttt agcaagaaat cagtcatttt acgaacaaga ctttgttcgt
     2521 ttcttgcaaa agaaaatcag gcaaagtaat tttttgattg tcaatcatgc gtttttagta
     2581 caagaaacac agcgaaaaga accattgctt ccagctagtc catttttgtt gatcgatgaa
     2641 gtacatcatt tgccggaaat cgctgaaaaa gtaaatgaca aacgattaag tattaactat
     2701 ttccataaac aaatccagca attagaagaa ccaaatcagc tgtttgaccg aatacaaagc
     2761 tatttaccga aagaacatga agccttacgc tggttatcga tttatcgaga agaactttct
     2821 gcgctatgcg aagcacagat ttgtatggca gaaggattaa tgagtttagt gagcggcaag
     2881 caacctaacg gacagtatcc agaagaagtc atgatgacaa aagagatgtt ggatcagctg
     2941 ccactggaat ctgggacagc cattgatcac ctgcagcttt tttatcagga gatccagcag
     3001 ctacagtcac aaatgcgtct gacttttgag attatccaag aaagctggac aaaccagatg
     3061 cggatcgatt tagcaagttt atatagccta tttgatcaat tagacaagca atacttgctg
     3121 atggatcaat ggctggaaga ctggcgagat catttgatcc attggttcgt tcctgggaac
     3181 acagcgcagc agatgatttt tcatctccat gattttcaag cggctatgtt gccgtcgact
     3241 gtttggtatc ctcgatatga tcgtattctt tatattggag gaacgctgaa gatcggccct
     3301 aatcgacact atctctctaa gaggcttggc attgaagcag cacctcttaa agtgattgcc
     3361 tctccttatg attatgagaa acaggcaagg atctatgtgc caaaagaggc acctgccgtc
     3421 catcaagtca gcaatcaagc atatatcgat tatttagaga cagcacttac aggtctgatc
     3481 gaacaagaag atcggccaat acttgtgtta ttcacttctc atgatgtttt gcagaaggtc
     3541 tatcagaaaa tgcatctgtc tatgctagaa aaaggacgag aaatactagc tcaagggttt
     3601 ggtggaagcc gggagaagct tttgaaacgc ttcattttgt cagaaaaaag cgtattgttt
     3661 ggcgctgaca gcttttggga aggtgtagat ttgcctggag atgctttgca gatcgttgta
     3721 gtcacacgtt tacccttcga taatccgctt cgcccgatgg tcaaagctag gaacgcttat
     3781 ttagaatcag aggggatcaa tcctttttac caggaatccg tacctaaagc agctttgaag
     3841 ttacgccaag cattaggtcg cttgataagg acggagacag atcgtggcgt attgcttatt
     3901 ttggatcgaa gactagtcac tgcaaaatat ggcgcacgga tcatccatgc attaccgaaa
     3961 aaagtaccga tcaaagagct agctatggag gaaatctatc aggatatcca tgaattctta
     4021 aagaaaaatg aagaaggaag ttagaggagc tgtctttttt cataattaaa atctgctata
     4081 atgaaaagga atgaagataa aggagcgacg acccttgaag aaaaaacaga caaaggaaaa
     4141 aacgattaat cgcgtgttac ttggcttgat cgtattttta ttagcggtca tcgtgatcag
     4201 ctcgattttt tacttgcgtt ccagtcgtcc ggcggtgcag gcgaagaaag aagcagtaga
     4261 aatcgcaaag aaatatgcaa atcttgagga aaccgatcat ttttattggt ttactcgaaa
     4321 gaagacatac ttttcactga caggtacaaa tgataaagga aaagaaatcg ccgttatcat
     4381 tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca aagggactga ctgaaaacga
     4441 agcaaaacag acaattgctg cggcccatcc tgaaatccaa atcgaaaaag ctgctttagg
     4501 gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg ggaaactgaa
     4561 ttattacctg attggctttg aatctggaga tgaagtcaag acaatcaagg gaatttaaaa
     4621 ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa aacgattaga accgtctgtc
     4681 actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga tgtgctgagt
     4741 ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc agcaatcgaa
     4801 gcaatccgca atgggaaagc tagctattat acaccaacag caggaatccc agagcttcga
     4861 caggcaatcg ttgattatct gaagaaaaac tatggacttg tctatgaacc ttcacaaaca
     4921 gtcgtaacag acggagcgaa atttgctttg tatacccttt tccaaacgat ccttgacccg
     4981 caagatgaag tcatcattcc tgtgccttac tgggtcagtt acggtgagca agtaaaactg
     5041 gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa agttactgtt
     5101 gaacagctag aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa ttctccatcg
     5161 aatccgactg gaatgattta tagcaagaat gaactgcagg caatcggtga atgggcagta
     5221 aaaaatgata ttttgatagt tgctgatgat atttatggcc gtttagttta taatggcaat
     5281 gagtttactc caattgcaac gatttcagaa gcaatcaaaa atcagaccat catcatcaac
     5341 ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg gttatgcggt gggaaatcct
     5401 gtgatcatca atggaatgat cgctgttgct tcccaatcaa ctagcaatcc gacagcagtc
     5461 agtcaatatg cagctgttga agcattgaaa ggtgagcaag atacagtaga agaaatgcgg
     5521 aacgcctttg aagaacgcct gaatacgtta tatccgcttg tggcagaact gccgggtgtt
     5581 tctcttgaaa agccgcaagg agctttctat ctttttccta atgtaaaaga aacgctacgc
     5641 atgtgtaagt atgaaaatgt cacacaatgg gtagaagatc ttttagaaga aacaggggta
     5701 gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg tccgcatgag ttatgccact
     5761 gatcgcgcta cgttagaaga agcggtacgc agaatcaagc aatttattga gagtaaaagt
     5821 caaaattaat cctgaagaaa ttcaaggaat ataaaaggag agtcatacgt ggaacaaatt
     5881 caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc ttgggtcgca
     5941 aataagcgat caagcggaaa aatcgcattt ttacaattaa gagacggaac agcctttttc
     6001 caaggtgtag ttgtaaaaag cgaagtgtcg gaagaagttt tccaaacagc taaaaatcta
     6061 caccaagaaa catccgtgtt agtaactgga gaaatccgtg aagacagtcg ttcaaaattt
     6121 ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg agagccacga ttatccgatc
     6181 acaccaaaag aacacggaac agatttcttg atggatcatc gtcatttatg gctgcgttct
     6241 tcaagacaac atgcgattat gcagatcaga aatgaaatca ttcgtgcaac gtatgaattc
     6301 tttaatcaac atcattttgt caaaatcgat ccgccgatct tgactggttc tgcaccagaa
     6361 ggcactacgg atctattcga aacaaattac tttgatcaaa aagcttattt atctcaaagt
     6421 ggccaattat acatggaagc tgctgctatg gctttcggaa aagtcttttc attcggtccg
     6481 actttccgtg ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg gatgatcgaa
     6541 ccagaaatgg ctttcactca tcaagaagaa agtttagaag tacaggaaca atatgtagca
     6601 ttcttagtac aaaatgtttt agatcattgc gaatatgcat tagatgtact aggacgagac
     6661 aaagaagtct taaaaactta tacaaaattg ccattccctc gtattactta cgatgatgca
     6721 atcgaattac tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga ctttggttca
     6781 cctcatgaaa cgttcattgc gaattcattc gaacagcctg tctttatcct aaactatcca
     6841 aaagccatca agccattcta catgaaacca catccaacaa gagaagacgt ggtaatttgt
     6901 gcagatatga tcgcaccaga aggttacggt gaaattattg gcggaagcga acgggcaaca
     6961 gactatgatt tcttgctaga acaaatccgt aatcacggtt tggatgaaaa agaatattca
     7021 tggtatcttg atctacgtaa atacggttct gttccgcact ctggttttgg tctaggcctt
     7081 gaacgaacag tcacttggat tgctggtatc gaccatgtac gtgaagctat tccattccca
     7141 cgtttgttga accgaattta tccataagag gtgatgaaaa agctgttctg catcaagtaa
     7201 ttagaacagc caagcaaaaa cagctgttca tgtttttgaa ttggcgggac ttattaccgc
     7261 agatgtaagc ttttgaatac cgtttagtgg agaggttgtg aaaaagctgt ttagtttcga
     7321 gtattaagaa aaattttgtg gaaatggctt tccacatttt tgcataaatt tggcttaata
     7381 ccgagaaact ggcttttgaa caccgtttaa ggaagaggtt gtgaaatcag tgccttgacc
     7441 tgagtattag agaaacaaaa cggaaaaata gcttctcata ttttaccgtt tttttagcta
     7501 atatcgaggg tcagctgatt gaacaccgtt tatatcaaga ggttgtgcgc ctgccgttca
     7561 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7621 cttgtgaccg aggagttggc cggagagcaa cgtttattta aaaaaaagaa ctgcgaactc
     7681 tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag
     7741 caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat
     7801 atacaaagaa ggttattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa
     7861 agcagcatct agtacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt
     7921 attttcagca tgcaatgatc catatgtaag tttaattcat ccttgaaatc atcggaccag
     7981 ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa aagaacattt
     8041 actggaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat
     8101 tgacggaagc ttaggtgtga tcgatttctc tggaccaaac aacgaaggaa tcaaggggaa
     8161 tccggcacgt taagaaaaaa agtataaaaa actagacact tgatgagcgt tcattcaatt
     8221 gattgaaggg actaggctgg tgtcattttt tatacatata ttttctcttt cgtcttgata
     8281 gggcttatgg gataatataa ggaagaaaat catcgtttta ttcaaaaagg agtttttgac
     8341 aaatgaaaaa acgtatatta tttatcggtg acagcattac agacgcagga agagaccgag
     8401 cagaagcaac tgatctaggt aaaggttatc cacttttgat tgccggaaaa ctaatctccc
     8461 aatatccaac gatgaaattt gaatgcatga accgtgggat aggaggagac aatattgcag
     8521 atctgcaagt acgctggcaa gaagattgtt tagctttaca acctgactat gtatcgattt
     8581 tgatcggaat caatgatacc tggcataata ctagcgatca ggctatattc ggaactgaga
     8641 aatctgcgca gcaatttgaa tccgtttatc gaaaattact aaatgaactt caaacagcgg
     8701 aaatctgcaa catcatcttg atggagccgt ttgtacttcc tataaccgac gaccgtcatg
     8761 gctggcgtaa agacttagac cctaaaatcc aaattgttcg taaacttgct aaggaatatg
     8821 gcgcattgct ggttccttta gatggcatat tcaatgcttt agggattgct tatggttttt
     8881 cgaaatatac aagagaagat ggggtccacc caacgattgg cggacatgag atcattgcac
     8941 aaagctggat agatgcaatt gccgcagtcg atttttttag tggagataaa aatgagcgaa
     9001 agaaaagtga gaaatgatgg aaatacggtg ggcagtaaaa gaagatttac ctcaaatcaa
     9061 tcagctgatt actgatcatc cagaatcatt cgtaaaagca aaaacggcag ctgacctaaa
     9121 tgataagaat aaactattgc tcgttgctgt aacaaaagaa aaacggatta tcggatttgc
     9181 gcaagcggac gtgcaacagt cagaagaacg ggtggatcag cgaccatcag gaaaactaat
     9241 ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc caaaaaaaat ttgcagaata
     9301 cttgatgctg gaagctaaaa aaagaagaat aaaatcaatt tttgcccagt tttaatcaaa
     9361 aacagtaata gaaggagaaa tacaatgagt tgtttaggaa atgtcatctg gtttatattc
     9421 ggcggactaa tcggtggtct tagctggtta gcagcgggga tactttggtg tatcacgatc
     9481 ataggtatcc caatcggtat gcaatgtttt aagatcgcct ccctttcttt tgcgcctttt
     9541 ggcaaagaag taatttatca ccagtctggc atgtcattga tcgttaatat catctggctg
     9601 atcgtttcag gcctaccaat tgctctcgca catgtcgtca gcggcatcct tctatgtatc
     9661 acgatcatag ggataccttt tgccaagcaa tctttcaaac ttgctcgatt ggcgttgatg
     9721 ccattcggtg cagaaattgt ataaaaagag aataataaag gttagaaaac gcatagagtc
     9781 aggtgggata catgtatttc acctgactct atgtgttttc ctgtataagt tctcccatgt
     9841 gacttttacg tcttttattt atatgggatt actgtttatg gaaagttgaa ataattttca
     9901 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9961 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
    10021 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
    10081 gttggataag agcaggggca atgatcgaag agaaaaagaa agaataagcc aaaacatctg
    10141 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10201 tgaataacca ttcatttata attagttatg aagagatgtt catatgttga ggcgaggagg
    10261 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10321 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10381 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaacaaaaa
    10441 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10501 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10561 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10621 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10681 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10741 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10801 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10861 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10921 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10981 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    11041 ttggtgaaac agtattaagc ggatttatca ataaaaataa accattttta gttcaagtag
    11101 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11161 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11221 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11281 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11341 tgatttccgt gccacttagt ttctttggcg gtatcggagg cgcaagtaaa atcggtgtat
    11401 tagtaaaagg ttcaaattac ttggagttgc ttgcgcaaac cgagacagtg gtattcgata
    11461 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11521 caaagaaatt catgcaatat attgcgagtg cagaacagtt ttctacacat ccgattgctc
    11581 aatctgtttt agaaggctac gcaggtccgc ttttaccaac aactaatatc caggaatttg
    11641 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11701 tgatggaaag atttgaaatc tcttttcctt taagtcaaga aatcggtaca ttgctttatc
    11761 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11821 ccgtagacgc attggttcaa ttaaaacagg ctggtgtgaa gaacacagtc atgttgacgg
    11881 gcgactcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11941 gcgaattgct tcctgaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    12001 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    12061 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12121 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12181 aaatcgtcaa acaaaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12241 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12301 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa agaggacgaa tgaacgtcct
    12361 ctttttttta tatccttttc ttaatgaaaa atgtaaagaa aatagatagg attataaaat
    12421 aactaatgtt tttaattttg cagaaaattc aaaatagctt gaaattcaat aaggtcaatg
    12481 aaataaaaga aaataactta tttttttgat ggaaaaaata tgaaaaaacg atgtgtatta
    12541 aataaaagaa tacacacata aaataacact gtcttgtaag tttgttaaaa attgaatatg
    12601 attacatctg gataacaaat ttttatcata aaggagagaa cgaatgaaca acaaacaatt
    12661 acgctggttt actgttgcgt taattgcatt taacatggtt tggggcatgg ggaatgtagt
    12721 taataactat gctcagcaag gagtatcagt tgtcacctct tggctattga ttttggccat
    12781 ttattttatt ccatacgcat tgatcgttgg acaattagga tcagccttca aggatagtaa
    12841 aggaggcgtc agttcatggg tcgaaaatac gacatctaat aaacgactgg catactatgc
    12901 cgcttggact tactgggtcg tccatattcc ttatttggca caaaagcctc aagcaattct
    12961 gattgctggt ggttgggcag tccaaggaaa tggaaatatc gtaaatacga tgtcagtaca
    13021 aatggtagca gttatttcac ttattatatt tcttgccttt ttatatttat caacaaaagg
    13081 actttctact ttgaaagtga ttggcggact ggccggaaca gcgatgttcg tcatgtcgat
    13141 cttatttatt cttttagctg taacggcacc gttcatcaat ccaacgatgg aatttgccac
    13201 accacatatg aatcaagtga aaacatatat tccaacattt gatttcagtt atttcacaac
    13261 cgtttcgatg ctagtatttg cagttggagg ggcggaaaaa atatcccctt atgtcaactc
    13321 tacaagaaat cctgcaaagg aatttcctaa agggatgatc ttcttagcag caatggtagg
    13381 gatttgtgct gtattaggct cagtcgcaat gggtatgctg tttgctagtg ataatattcc
    13441 taaagacttg atggcaaatg gggcatacag tgcttttcaa attttaggta attactatgg
    13501 tatcggtaac tcattgatga tcatttacgc attgacaaac atgattggac aaatctccgc
    13561 attagccttt tcaattgatg caccactaaa aatcttgtta gcagatgcgg atccagaatt
    13621 tgtaccaaac tggctacgta aacgtaatcg taaaggtaca ttggtgaatg gttatcttct
    13681 gacaggtata ctagtaggaa tcattatttt gttgccgatt tttggtatca aagatatgaa
    13741 tgaattagta aaatggttga cgaacttgaa ctcagttgtc atgccaatgc gttacctatg
    13801 ggtgttcttg gcatatatga tgttgaataa agcttataag aaattcaatt ctgaatataa
    13861 atttgttaaa aatccaaaaa tcggttttat ctttggtgct tggtgctttg ccttcactgc
    13921 gtttgcatgt atcctaggaa tggtgccaaa agtagattat actgctgatc ctaaagcatg
    13981 gtggttccaa ttatttacga atatcatcac accaatcgtc ttgatcttat taggtatgat
    14041 cttgccagca attgctcgtc gagacaaaaa caaagaaatc actgaaccag aaacagtggt
    14101 tcagccttaa aatagaaata cttgttacaa gaggttgtga cagaagtggt cagcttcaag
    14161 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaagtttc ggcttgtttt
    14221 tcgaagaagc tacttctgga acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc
    14281 aagtaataag aacagccaaa caaaaatagc ccctcatatt tttcgtttgg ctgggcttat
    14341 taccgcagat gcaagctttg gaacaccgtt taaggaagag gttgtaaaat cagcgatttg
    14401 acctgagtat tagagaaaca aacggaaaaa tagctcctca tattttgccg ctttttgagc
    14461 taatgctgaa ggtcagctga ttgaacacca tttatt
//
LOCUS       DAFBQW010000065        14409 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFBQW010000065 DAFBQW010000000
VERSION     DAFBQW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.068"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..135
                     /locus_tag="LK889_002287"
     CDS             <1..135
                     /locus_tag="LK889_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5096491.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="LK889_002288"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="LK889_002288"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK5096492.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="LK889_002289"
     CDS             complement(2412..2663)
                     /locus_tag="LK889_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBK5096493.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="LK889_002290"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="LK889_002290"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBK5096494.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="LK889_002291"
     CDS             3613..3831
                     /locus_tag="LK889_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096495.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="LK889_002292"
     CDS             complement(4120..4632)
                     /locus_tag="LK889_002292"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK5096496.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="LK889_002293"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="LK889_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK5096497.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="LK889_002294"
     CDS             complement(7085..7528)
                     /locus_tag="LK889_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBK5096498.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="LK889_002295"
     CDS             complement(7529..8317)
                     /locus_tag="LK889_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5096499.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="LK889_002296"
     CDS             complement(8314..9255)
                     /locus_tag="LK889_002296"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBK5096500.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="LK889_002297"
     CDS             complement(9432..10019)
                     /locus_tag="LK889_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBK5096501.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="LK889_002298"
     CDS             complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="LK889_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBK5096502.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="LK889_002299"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="LK889_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK5096503.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="LK889_002300"
     CDS             complement(12936..13871)
                     /locus_tag="LK889_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5096504.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14057..>14409
                     /locus_tag="LK889_002301"
     CDS             14057..>14409
                     /locus_tag="LK889_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096505.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT         4036 a         3197 c         2538 g         4638 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt aaaattaagt
    13981 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
    14041 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
    14101 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
    14161 acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg
    14221 gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca
    14281 tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg tcattatcgg
    14341 gcgaaaagaa aggtctgttt catgcggtat gagaacactt tatcgtttat tttctaaagg
    14401 aatatttga
//
LOCUS       DAFBQW010000066        13999 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFBQW010000066 DAFBQW010000000
VERSION     DAFBQW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.069"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1283)
                     /locus_tag="LK889_002302"
     CDS             complement(<1..1283)
                     /locus_tag="LK889_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5096506.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
     gene            1462..2313
                     /locus_tag="LK889_002303"
     CDS             1462..2313
                     /locus_tag="LK889_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5096507.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2367..4118
                     /locus_tag="LK889_002304"
     CDS             2367..4118
                     /locus_tag="LK889_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5096508.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            4122..4292
                     /locus_tag="LK889_002305"
     CDS             4122..4292
                     /locus_tag="LK889_002305"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5096509.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(4332..5234)
                     /locus_tag="LK889_002306"
     CDS             complement(4332..5234)
                     /locus_tag="LK889_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5096510.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5465..6868
                     /locus_tag="LK889_002307"
     CDS             5465..6868
                     /locus_tag="LK889_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5096511.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6868..8301
                     /locus_tag="LK889_002308"
     CDS             6868..8301
                     /locus_tag="LK889_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5096512.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8446..9372
                     /gene="trxB"
                     /locus_tag="LK889_002309"
     CDS             8446..9372
                     /gene="trxB"
                     /locus_tag="LK889_002309"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK5096513.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9586..11313
                     /locus_tag="LK889_002310"
     CDS             9586..11313
                     /locus_tag="LK889_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBK5096514.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11328..11678
                     /locus_tag="LK889_002311"
     CDS             11328..11678
                     /locus_tag="LK889_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK5096515.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(11770..12630)
                     /locus_tag="LK889_002312"
     CDS             complement(11770..12630)
                     /locus_tag="LK889_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBK5096516.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12722..13150
                     /locus_tag="LK889_002313"
     CDS             12722..13150
                     /locus_tag="LK889_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5096517.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13312..13488
                     /locus_tag="LK889_002314"
     CDS             13312..13488
                     /locus_tag="LK889_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK5096518.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13515..13961
                     /locus_tag="LK889_002315"
     CDS             13515..13961
                     /locus_tag="LK889_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBK5096519.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4522 a         2425 c         3080 g         3972 t
ORIGIN      
        1 ttcttagttt tcttgaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
       61 aagtttctaa agccataggc aattcgtttt agtactttga tatggttgtt caaacattct
      121 atagggccgt tggaataggg aactcgcaac gctcgtttga tttctgttcg atatttcttg
      181 aaggtcgtga acactggttg atagtgctct ggcaactcct tgtagtcttc ttttaggagc
      241 gcgacgaatt caggaacatc ttgtcttctg atagcggaaa gaaaaacctg gtagacttcg
      301 tagcctcgct ttaaggagtc atcataatat aatagccgat cgactatctc cgcttctgtc
      361 agatgatctc gaaatgacgg acgccagtag cgatgctgac cattcaattc ccacgcattc
      421 ttttgaagta gtctccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
      481 gtcttcatta cttgaattcg gtggttggta aaggcccgac cgatgtgttg gctaatatga
      541 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
      601 gtatagaaat ctgaaaccac tagacgtact tgttcacgga cttttctagg gtagcgcaag
      661 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
      721 ttgccgtcca tcatgacaaa gctcatggct cccgagacgt ttttcaccga tttgaattca
      781 tcgaaacaca ccacttctgg taacggctgg ttcaaagaaa cggttttagg ccgaaggctg
      841 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcaat caaagacata
      901 gaaataggct cacttaatag ctcaagaatg gcttgtttga ctctgcgggc aatggagcag
      961 cgacgatcgc taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     1021 cgctgtttgt taatccgcac aatggtcttg tagttactga catcattcaa taagatggat
     1081 acttttttcc agccccattt aatgatcgtt tgcttatttt gatagtggca atggaagcat
     1141 tcctctggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attaatgcgc
     1201 tctttgcgaa aggcatcttc atgaaaggta agatttaggt ctagaatatc tagtaactct
     1261 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     1321 ttctaaagga tttcaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     1381 cagaaggcgt caccaacact agatattata gatccttttt gttttggata cagccctata
     1441 catcatggaa aggagcaaaa catgctgatt gagaaagtac tgaataacaa cgtcgtaata
     1501 accagggatg aaagaaatca ggaaatgatc gtgatgggaa aaggtttggc attcaaaaag
     1561 aaagtgggtg acccgatttt tgactcatgc atcgacaaaa aatttcagtt gacaaatgac
     1621 gggctttcac aacagttcca agaattggca caagcgattc ctcttgagta cttagaaatt
     1681 tcttgtgaaa tcattgaata tacaaaagag gctctacaag tccagttgaa tgattctgtg
     1741 tatattgcat tgacggatca tttgtatacg gctatcgaac gtcacaaaat gggaatattt
     1801 gtaccaaatg tattgctgta tgatatcaaa caactatttc ctaaagagta tgccatcggg
     1861 aaaaaaacag tgaagaaaat ctcggataaa taccacatca agttatcaga agatgaagcc
     1921 ggatttatcg cattgcattt agtcaatgca caaggcattc aaagccagca agacaatacc
     1981 tacctgttta ctataacaat acaggatatt ctaaatatcg tccgctatta ctataagatt
     2041 acatttgatg aagattcagt ccattttttt cgattcgtca cacacttacg tttttttctg
     2101 acacgcgtcc tagatggaga aaaatcagaa gtgggagaag tttctgagga attgtttcag
     2161 gttattcaaa aaaaatataa ggatacgttt ggttgcgttg agaaaatagc agacttttta
     2221 gccgagacgt tcaactacca gatgtccacg gacgaaaaaa tgtatttaac gatccatact
     2281 gcgcgtttag tgcatgagaa tcgttgtaaa taaaaaaagg aggataaatt aacaagaacg
     2341 tatgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa ttggagggaa
     2401 tagaaatggg aaaataccaa gaattagcga gaaaaatcgt agaaaatgtc ggcgggaagg
     2461 aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta aaaaacgaag
     2521 aaaaagcaaa tgatgagatc ttgaaaaata tggacggtat tgtaacggta atgcgagcgg
     2581 gagggcagta tcaagttgtc attgggaatc atgtgccagt cgtgtttgaa gaggttataa
     2641 aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga ccgtttgata
     2701 tgcttattga tggaatatcg ggctgtttcc agccattcct tgctatccta gctgctggcg
     2761 ggatgattcg tggactgaca gcattccttg tcttcttagg cgcgtttgac cgcggttctg
     2821 gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg cctgttatta
     2881 tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg ttgcttggcg
     2941 cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca gaagcaccac
     3001 taacgaccat attttctggc actatttttg aagcacctat ttatcaaaca gtattcggca
     3061 ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt attgtgttgt
     3121 tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc gcgaatttct
     3181 tcgttccatt cttcaccgtt cttattacaa tgcctttagg ttttttactc gttggaccag
     3241 tattcacatt cgcaactgat attttaatgg ctggatttga aacactttta gcactttcgc
     3301 cagttattta tggagcaatc gtaggcttct tctggcaaat tctggtgatg tttggtctcc
     3361 attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg caaaatatta
     3421 tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttaccgca ctctacttta
     3481 agcttagaaa tccaaaagac aaagcaattg caataccagc cattgtttct ggtattgtgg
     3541 ggattacaga gccagctatt tatgggttta cattaccacg taaaaaaata tttattttta
     3601 cctgtgtcgg tggtgcgtta gggggcgcgt atagtgggtt aatgaattta acttcttgga
     3661 atcaaggtgg actaggcatc tttacaattc ccaactatat tagaccagat ggtgatttaa
     3721 cagatgtgat taatgtattg attgggatcg ccattgcgat ggtggtatcc tttacgctga
     3781 cttttttctt ttggaaggat gaagcaggtg aaacagatga tatacagaaa aaatcaggta
     3841 aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actaaatgca gcaaaggatg
     3901 cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa aaaggagaag
     3961 tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacag gcaattggaa
     4021 tggtttctga aacagggctg gaattgctga ttcatgtcgg attggatact gtgcagttgg
     4081 aagggaaata ttttgaaagc ttagtgcagc aaggataaag agtgaaacaa ggcgatctgt
     4141 tgctacgctt cgacaaaaag aaaatcgaaa aggaaggata cagtttagaa acacctgtga
     4201 tcgtcaccaa ttacacggat tatttggata ttttggaaaa ccaaggggag acggtcgggc
     4261 cagacgatgc acttatcacc gcactgacct gaacggttag gtgaagaatg gactaggctt
     4321 aactattctt tttattccac ttctttattt tcaaaattga aaggctcttt catgatggct
     4381 gagctgatag ggcgtttatc gaatttttga ccgacagaaa ttagatggac taaattctgt
     4441 tgataatttt ctgcaaaaac acaactatat aacacgtcta gaagataaca aattgatgta
     4501 ttgatcgtaa aattaccaat ttttgaatac agtcgctcgc gtgtcgtaag tcgcaaagcg
     4561 gctgtactca ttgcagatag cgttgaatca ccgatactgg tcaaagctaa aattggaaca
     4621 tgattttgtt tcaatatttc tgcggtacga aggatcacat tgttttcacc cgtataagaa
     4681 atcagaatcc cgcacatatc actcgtgcaa ttgtacgctt catagaatga ttcaccaaaa
     4741 gtggtgacaa tcgtgacatc cattttgatg cgcttcattt tcagcatgaa atcttgagaa
     4801 atcaaagtat tcgagttact ggcgaaaatc acgatttttt ttgcttgaag cattagttgt
     4861 ttggcttttt gcagttggtc atgatgaatg agagaaagtg tatcatgaat cgtcatctgt
     4921 tctaaagaag ccattttatt tgcgatcgtc ataataccgt ctgtggtgga aaaaggaaga
     4981 ttcgcatcta cgtcttgaaa attcccttca agataggctg tttcagctaa aaaagcatta
     5041 cgaaaactga cccagccatc aaaacccatt ttttttgcta cacggatgag tgtagagggg
     5101 tggacataac atcgctcagc aatcgcttgg atggtcattt cgccaagtgc tggagaatcg
     5161 cttaagagaa actggatcac ttcattttct gaaggagaaa aggagatttc tttgattttt
     5221 tcacgtaata acatgtagat accttcttct tattttttta cgtaaattat aacttttttg
     5281 aaaatgattt gtaaacggat ttatactata taacttaaaa tagacatttt ttgacaagat
     5341 atttacaaaa atccactaga aaacagtaaa ttttgtaaat gttactggaa agaaacagat
     5401 ttctattgtt tgttttgttt acaaaaaaga taatgaaagt gtaaaaaagt aaaggagtga
     5461 gaacatggca aaaggaatca aaatcgctac gattggtgga ggcagcagtt acacccccga
     5521 gttgatggaa ggatttatca aaagatatga ggaattaccg atacgagaaa tctggctcgt
     5581 agatgtagaa gaaggaaaag aaaagcttga gatcgttagt cagatgtctc agcgtatgtg
     5641 ggatgcttct ccgtatgaag tcaagatcta tgcaactctg gatcgggaag aagcattgaa
     5701 agatgcggat tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac gaatcaaaga
     5761 cgagcggatc cctagttatt atgggatgat tgggcaagaa acaaatggtg ctggcgggat
     5821 attcaaagct tttcgtaccg ttccagtcgt tttggcaatc gttgaggaca tgaagcgttt
     5881 atgtccaaat gcttggctga tcaatttcgc gaacccaagt gggatggtga cagaagcagt
     5941 tatcagatat ggcaaatggg ataaagtgat tggattatgc aatgtgccag tgatggcgat
     6001 gatgacagaa ccagcaatgc ttggcaagaa aaaagaagag ctgatctaca agtttgccgg
     6061 tctaaatcat ttccattggc acaaagtagc agataataaa ggaaatgacc gcacggcaga
     6121 attgattgat cgtttatacg atgaagagaa tggattaccg aaaaatatct ttgatatccc
     6181 gtttttcaaa gaacaattaa aacaaatgaa catgatccct tgtggctacc accgctatta
     6241 ctatcgtgaa gaagaaatgc tggaacatgc tttagaagaa tatcaaacga ttggtacaag
     6301 agcgcagcaa gtgaaagaaa cagaagcaga gctatttgaa ttatacaaag atccagaatt
     6361 agaccataaa cctgaacaat tgcaaaaacg aggtggcgct tattattcag acgctgcctg
     6421 tgaaacgatt gcttcaatct atggaaataa agaaacacag atcgtggtat ctacaaagaa
     6481 tgatggagct gttcctgacc tgccggcaga ttgtgtagta gaagtcactg cctatgttgg
     6541 cgcacaagga gcgagaaatg tagcgtttgg ttctcttcct gctgctgaaa gaggctggtt
     6601 gcaagtaatg aagaatatgg aattgctgac gatcgaagca gctgtgactg gtgattatgg
     6661 gacagcttta caggcgttta cgatcaatcc acttatacga tcaggaaaga cagcaaaacg
     6721 gatcatggat gagttgttta ttgcacacca gcagtattta ccgcaattca aggagacaat
     6781 cagccgatta aaagaagaag gaatcgaagt tcaagatgaa ctggcaagag aattatcagg
     6841 taatacacat ataatggagg aagtataatg ggattcagaa aagatttttt atggggcggt
     6901 gctacagcag ccaatcaatg tgagggcggt tatgatgaag gtggacgcgg actagcaaac
     6961 gtagatgttg tgccaatcgg aaaagaccgt tttcctgtta tcaccggaaa gaaaaaaatg
     7021 tttgaatttg atgaggatca tttttatcca gcaaaaattg caatcgatat gtatcatcgt
     7081 tataaagaag atattgcttt gtttgcagaa atggggttca agacttatcg aatgtccatc
     7141 gcctggagtc gtatttttcc aatgggggat gagaaagagc cgaatgaagc tggcttgaaa
     7201 ttttatgagg atgcctttaa agaatgccaa aagcacggaa ttgagccact tgtgacgatt
     7261 acgcattttg attgtccaat gcatttgatc aaagaatatg gtgcgtggcg ttccagagaa
     7321 ctcgttggtt tttatgaaaa tctgtgtcga gtcatcttca atcgctataa aggacttgtc
     7381 aaatattggc tgacattcaa tgagatcaat atgatccttc atgcaccatt catgggcgca
     7441 gggctgtatt ttgaaaaagg agaaaatgaa gaacaaatca aatatcaagc ggcacatcat
     7501 gagttggttg caagtgccat cgcaacgaag attgctcatg aagtagatcc ggagaacaaa
     7561 gtaggctgta tgctggcagc aggagcatat tatccataca cttgcaatcc tgaagatgtt
     7621 tgggcttcta gaaaatcaga tcgagaaaat tatttcttca ttgatgtcca gtcacgagga
     7681 gaatatcccg cttatgcatt gaaagaaatg gagcgaaatg gcattcagat cgaaatggaa
     7741 ccaggggatg ccgcattgct gaaagaacat actgttgatt ttatctcttt ttcttattat
     7801 gcttcccgag tttcgactac cgacaacgaa ctcttggaac agactgcagg aaatatcttt
     7861 gcttctgtca aaaatccata tctgcaagca agtgaatggg ggtggcaaat tgatccatta
     7921 ggcttgagaa taacgatgaa tgatatttat gatcgttatc aaaaaccatt gtttattgta
     7981 gaaaacggct taggtgcagt cgatacgcca gatgaaaatg gttatgtgga agatgattac
     8041 cgaatcgatt acctggcagc gcatatccag gcgatgaagg atgcagtaga actagacgga
     8101 gtcgatctat taggctatac gacttgggga tgtattgatt tagtatcagc tggtaccggt
     8161 gagatgaaga aaaggtacgg attcatttat gtcgatcgtg ataaccaagg aaatggcaca
     8221 ttagcccgtt caaagaaaaa atcattcaac tggtataaaa aagtaattga ttcaaatgga
     8281 gaaaatcttt cgaacgattg atcaatgatt tataaaagtc tattgttttt cagcggattt
     8341 ttcgagtata ataaagctat tcgagaaacg caagcagaac aatagacttt ttttcgcatt
     8401 ggctgtaaag tatgcttact tgattaagta aaggagaatc tacatatgta tgatgtaatc
     8461 gttattggcg caggtcctgc tgggatgact gctgctttat atgcttcacg ttcaaactta
     8521 tccgtattga tgatcgaaca aggtgcccct ggcggccaaa tgaacaatac ggcagaaatc
     8581 gaaaattatc ctggattcga ttcgatcatg ggccccgatc tagcttataa gatgtatgaa
     8641 agtgtctcaa gatttgggac agaaaatgct tatggaatcg ttcaagatat caaggatcac
     8701 ggcagccata aagaagtcat ctgtgaagat aaaagttacg aaggaaaaac agtcattatc
     8761 gcaacaggat gtatccaccg taaattaggc gtacctggcg aggaagaatt tgctggacga
     8821 ggcgtttctt actgtgcagt ctgtgatggt gcgtttttcc gtaacaaacg tctagtagtc
     8881 atcgggggtg gggactcagc agtagaagaa gccatctatt tgacacaatt tgcttctgaa
     8941 gtagtgatcg tccaccgtag agatgaatta cgtgcacaaa agatcatcca agaccgtgca
     9001 tttgcaaatg aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat cgttggaaac
     9061 gaaatggtag tgacaggtgt gaaagcacgt aatgtcaaaa ccggcaatga atttgattta
     9121 gcagcagacg gagtattcat ctatgttgga ctggatccat taactgcacc ttttagagaa
     9181 gcaggtttga cgaacgaagc tggctggatt ccaacagaca acgaaatgaa aacgaatatg
     9241 ccaggcgttt ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat cactacagct
     9301 gttggtgacg gcggtattgc tggacaacaa gtgtataaat acttggagtc tttagaagaa
     9361 acaacagcat aagtttaaaa agagtagaag aacgggttca attgttcttt tactcttttt
     9421 tttcgcgtga aatttactga atctgattta tgcgaacacg tccaacacct tttgataaaa
     9481 gaatcctttc ttgatttaag cgttttttgg tcctcatact ttcatatcta ataagtgaat
     9541 gctatactta ttaagaacca tatttcagaa agaggtgtta ttttcatgtc ttgggaacaa
     9601 gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa acttaaaaaa acaattaaat
     9661 gatctgggtg aagatcctga aaaacttgaa gatgcttttt atgcaccgtt ggaattcggt
     9721 actgcaggta tgcggggaat cttaggacct gggatcaatc gaatgaacat ctatacggta
     9781 agacaagcaa cagaaggact agcacgtttc atggatacac aaggtgaaga aacaaaacgc
     9841 cgaggtgtcg ctatcgctta tgactcacgc catcagtcac cagaatttgc aatggaagca
     9901 gccaaaacac tagcacatca caatatcccg tcttatgtat tcgaaagtct gcgtccaaca
     9961 ccagagcttt cttttgcagt tcgtcatttg aagacgtttg cgggaatcat ggtaaccgca
    10021 tctcacaacc cagcggctta caatggatat aaagtctacg gagaagatgg cggacaaatg
    10081 cctcctgcag acgcagatgc attgacgaaa tatgtccgtg aagtcagcaa tccgctaaaa
    10141 gtcgaagtat tatctgacga agaagctaaa catagcgggt tgatcacgat catcggagaa
    10201 gaagtggacg ctgcttatct agaagaaatc aaagcagtga cgattgatcg tgaattggtt
    10261 gaaacaatgg ggaaagattt gaaacttgtg tacacgccac ttcatggaac agggaaaatg
    10321 ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat tcgtactagt tcccgaacaa
    10381 gctgtggcag atccagattt cactacggtc aaatcgccaa atccagaaga acattcagct
    10441 tttgaatatg ctatacgact tggagaaaaa gaaggagctg acttgctgat tgcaacagat
    10501 cctgatgcag accgtttagg cgcagcggtt cgtttgccag atggttctta tgaagtattg
    10561 actgggaacc aaatcggagc attgtttgct caatatattt tggaagcaca tcaatcaaga
    10621 ggcactttac cagaaaacgc agcggtattg aaatcaatcg tttccagcga attgcctaca
    10681 gcaattgcgg aacactacgg tgctaccatg ttcaatgtgt tgacagggtt taaattcatt
    10741 gctgaaaaaa tccaacaatt tgaagaagac cattcacata cttttatgtt tggattcgaa
    10801 gaaagttatg gttatttagt taaacctttt gttcgcgata aagacgcgat ccaagcattg
    10861 cttttattag cagaagtagc agcttactat aaaaaacaag gcaaaacgtt gtatgacggc
    10921 ttgcaaacga tttttgaaac ctatggctat ttcgcagaaa aaacgatttc tgtgacaatg
    10981 agcggacaag aaggttctgc gaaaatctct gctttgatgg aaaaattccg taaagaagca
    11041 ccgacagaat ttgccggagc aaaagttatt caaacagaag actttaaaga attgacgaga
    11101 acagaccgtg aaggaaacgt agaaaaacta acaacaccgc catccgacgt attgaagtat
    11161 acattggaag aaaatggctg gatcgctatc cgcccttcag gaactgagcc gaaaatcaag
    11221 ttttatattg gtgtaaaagg aagctctaca gaagacgcag ccgaaaaagt agcctcttat
    11281 gaagcgatta tcaaaaagat gacagaagaa taaaaaactt gagactgatg gaaaaagtgg
    11341 taaactttag aagtcagtct tttttcaagt tggaggtgaa tggattgcca gacacgcaaa
    11401 aatcaaaagt agagaaagtc agtcaattat tcaaaatgtt gagtgatccc actcgtctta
    11461 aaattctgct atatctgaaa gacggggaac aaaatgtgac agcaatcacc caagcagtgg
    11521 aaatggagca gtctgctgta tcgcatcaac tacgtttatt aagagaaaat catgtcgtca
    11581 agtctcatcg cgagggaaaa gcgattttgt atagtcttga tgattctcat gtactggata
    11641 ttttgaacca gacactcaaa catgtggaac atcaataacg ataggtgaaa atagataaac
    11701 actggagtgt gacaacgaag aaatttcgtt gtcacactcc agtgtttttt aagaaaaaga
    11761 caaaaatgat taatcgcttt tttctgttgt ttcagttcca taataactat gatcttctag
    11821 gtctaaagca ttcaaaataa gcgaagccgt ttcttcaata gataaagatg ccacattgat
    11881 cactacacat cctagttttt cataaagatc attcgcaaaa tcgagttcag ctctaatttt
    11941 ttcgatatcg gaataagcag tatcagggtt taatccgtaa gagcgcatcc gttctttacg
    12001 gatattattt aaaatatctg ggttattcgt tagtcccacg atttttttag gatcgatttc
    12061 ccacagctgt ttgggaatat gtgcttgggg aatcaaaggt aaattagcaa cttttaaatt
    12121 tttatttgct aaaaacaagc tcaaaggcgt tttggaagtt cgggatacgc ctagcaaaac
    12181 gacatctgct tctaaaaaac cacgaggatc tttcccatca tcgtatttta cagcaaattc
    12241 cattgctttg atccgtttga aatagttctc attcaataaa tggagggcac caggttgccg
    12301 aattggcttg ataccagtta aacgttcaat ttctgatatc ggtggcgtca tcacatcgaa
    12361 actaaataat tgatgttctt cacaaaaaga acgtgttatc tcgatcagtt cttgattgat
    12421 taatgtatgc aggacaagtg ctttttcttc taatgcatct tgcaatgcct gccttaatgt
    12481 tttttcatct gtcacgaacg ttctgcggaa taggttgaac tcaacggtag gatactgagc
    12541 catagtagct tgcgctagtt ttgtcgcagt ttctccagca gagtcggaga tgacaaaaaa
    12601 agtaacgatt tctgtagaat cggaagacat ggataaaacc tcacttgcat tttttttatg
    12661 tttattatac catactctag aatgcgtact tcgtaatcat tttgatttgg aggaataagt
    12721 catgaaaaaa cagacattga tcgaacaatc attagaaaca ttgaaaaata acggttttaa
    12781 atatactaaa aaaagagaag cattgttaac ttatttagtg aagcgtaacc gctacgtatc
    12841 tgctaaagaa gtttttgact atatgaatca ggaattcaaa ggggtaagtt atgatacgat
    12901 ttaccggaat ctccatgatt ttgcagagat cgatttactg gaagaaaccg atttaaatgg
    12961 tgaaatgaag tttcgttttc attgctgtca gggggaaatc ggccatcacc atcattttat
    13021 ctgtacagtg tgcggaacaa cgaaagaaat ccatatgtgt ccaatgaatt atttcgaaga
    13081 acagctaggt ggatgcacga tcgaagggca tagattcgag attttcggaa gatgtgaaaa
    13141 ttgtcaataa ggatttaaat aaaataacat ttgataattt aaaaaaaaag caagatttcc
    13201 ttgacggtca aaacagcatt agatataata tttacaggac agtcttgttt tttgagatat
    13261 atttcaataa caattacggt tactttttaa gctcggaggg agggaattaa catgtcaaaa
    13321 acagtcgttc gcaaaaacga atcacttgac gatgctcttc gtcgcttcaa acgttccgtg
    13381 tcaaaagcgg gtactttaca agaatctcgt aaacgtgaat tctacgaaaa accaagtgta
    13441 agacgcaaga aaaaatctga agcagctaga aaacgcaaaa aattctagtt ctgtgatgaa
    13501 ttggagttgg tagtgtgtca ctactaagta gattaaacga tgacatgaaa gcagcgatga
    13561 aagcgaaaga taaggaatct ttacaagtca tccgaatgat caagtcatct atacaaaacg
    13621 agcagattaa agaaggtcac gacttgacag aagaagaaga actaacggtg ttgtctcgcg
    13681 aaatgaaaca gcgacgtgat tctttgcatg agtttgaaga agcaggacgt gacgatttag
    13741 ctgaaaaagt aaaaagtgaa attgtaatag tcgaaaaata tatgccggaa caactttccg
    13801 acgaagaaat tcgacaactt gttcaagaag caatcactca aactggtgct tcatcaatga
    13861 aagaatttgg caaagttatg ggtgcaatta tgcctaaagt caaaggaaaa gcagatggaa
    13921 accaagtgaa tgcgattgtg aaagaactat tacaatctta attttatcat ttaaactgcg
    13981 gacagtgatt gtccgcagt
//
LOCUS       DAFBQW010000067        13812 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFBQW010000067 DAFBQW010000000
VERSION     DAFBQW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.070"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..498
                     /locus_tag="LK889_002316"
     CDS             <1..498
                     /locus_tag="LK889_002316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096520.1"
                     /translation="LGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQPMNDKRNGHSWF
                     RQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDMTIENIPRIKEEI
                     ERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAIIDSGRVTQWKKI
                     QPFIF"
     gene            525..2336
                     /locus_tag="LK889_002317"
     CDS             525..2336
                     /locus_tag="LK889_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096521.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2397..3857
                     /locus_tag="LK889_002318"
     CDS             2397..3857
                     /locus_tag="LK889_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK5096522.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3868..6072
                     /locus_tag="LK889_002319"
     CDS             3868..6072
                     /locus_tag="LK889_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBK5096523.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            6088..6237
                     /locus_tag="LK889_002320"
     CDS             6088..6237
                     /locus_tag="LK889_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096524.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6313..6906
                     /locus_tag="LK889_002321"
     CDS             6313..6906
                     /locus_tag="LK889_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK5096525.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6919..7818
                     /locus_tag="LK889_002322"
     CDS             6919..7818
                     /locus_tag="LK889_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK5096526.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7821..8294
                     /gene="ssb"
                     /locus_tag="LK889_002323"
     CDS             7821..8294
                     /gene="ssb"
                     /locus_tag="LK889_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5096527.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            8660..8920
                     /locus_tag="LK889_002324"
     CDS             8660..8920
                     /locus_tag="LK889_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096528.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            9365..9571
                     /locus_tag="LK889_002325"
     CDS             9365..9571
                     /locus_tag="LK889_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK5096529.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9571..9822
                     /locus_tag="LK889_002326"
     CDS             9571..9822
                     /locus_tag="LK889_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096530.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            9838..10254
                     /locus_tag="LK889_002327"
     CDS             9838..10254
                     /locus_tag="LK889_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096531.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            10236..10508
                     /locus_tag="LK889_002328"
     CDS             10236..10508
                     /locus_tag="LK889_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096532.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            10675..11076
                     /gene="tnpA"
                     /locus_tag="LK889_002329"
     CDS             10675..11076
                     /gene="tnpA"
                     /locus_tag="LK889_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK5096533.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            11088..12233
                     /locus_tag="LK889_002330"
     CDS             11088..12233
                     /locus_tag="LK889_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5096534.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            12471..12734
                     /locus_tag="LK889_002331"
     CDS             12471..12734
                     /locus_tag="LK889_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBK5096535.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12728..13078
                     /locus_tag="LK889_002332"
     CDS             12728..13078
                     /locus_tag="LK889_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5096536.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(13102..13716)
                     /locus_tag="LK889_002333"
     CDS             complement(13102..13716)
                     /locus_tag="LK889_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5096537.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         5150 a         2269 c         2789 g         3604 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaacga acaaattgag
       61 aagatgtttg aacaccagag aaagaccaac ggtcagccaa tgaacgataa gagaaatggg
      121 cattcttggt ttaggcaaca agaacgttta gaaaacaaag tgcatagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggctataaca agtacggcgg cctggatatg acaatcgaaa atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ccgctacgat ccgaaaatac
      361 caacggaaat tggaaacatt agaacagctg aaagaaagat cagaaaaagg aaaagaaaat
      421 cttcttcctg aagtacaagc aattattgac agcggacgag tgacgcaatg gaaaaaaatc
      481 caaccattta ttttttgaaa gactatcgaa aagttgcttt agaactgaat gaaaacgggg
      541 gatttgagga atcaaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg
      601 aattacttga agaggctgcg ccaactgatt ctttgaagga gaaagaaaac gatcatttct
      661 atcgagtaga attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga
      721 tcgtaacatt agaattgatc gatcaactca aaacgttgga taaccaatac gccgcagaag
      781 caggttatta taaattctat tttgaagaag tgaagaacgg agaagtagtc gatcagatcc
      841 gaatggatct aggggatggc gtcaacagta atcaacccat ctatttagag ttagcaaaat
      901 tgtgcgaaca agtacctgaa aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga
      961 aagaacaaat gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg
     1021 cagaagagat taagcaagaa gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa
     1081 agcataccca ttctcctgaa gacgtgaaag aacttttgaa ttttatgtct cgttttccag
     1141 aacgatcctt tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt
     1201 taggtcgtgc tgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca
     1261 agatctttgt cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag
     1321 aaacagaagc tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca
     1381 aaaaacctta ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg
     1441 aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac
     1501 agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag
     1561 atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacggggg gcgtatgttc
     1621 gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc
     1681 atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag
     1741 ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc aaacattttg
     1801 gaatggacac ttcggagaaa gctattcctt atattgcagc atggacgaag aatggtcaga
     1861 cgttggatga taaggaagcg gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg
     1921 caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa agagaaccag
     1981 agatccaacc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg gcagagaata
     2041 agaatattgt gcccgttggt tcactttgga tcgacaagaa agacgggaaa gtaatggaag
     2101 aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat ccgcagcata
     2161 atcaagtgat tttaaaagga aaaagttatg gaaaagagat tcaaacggcg ttaccttcag
     2221 aagaatttgc gctaggaaat ccattccgat tttcacttga aacagtgtca gaagtggcca
     2281 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc gtataaaaca
     2341 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt ttgaaaatga
     2401 ccgaaaaaaa aatgagtctt attgatcgat gtaaacagat cgatatagtg gattttgctc
     2461 gaaacaatgg aatggctgta gtcaataaag gacgagacta tcgcttagaa gaccatgatt
     2521 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
     2581 acattattga attagctaaa ctttttttca tagacaaaga gatccaagac tctaagcaac
     2641 aatttaaagc agccacagat tttattctaa agaatgaaga caaaacagaa cgagtagaaa
     2701 atcttcattt tgagacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
     2761 aaaaaggaag gaattatctg aaggaagaac gaaaactacc agagtggctg attgattatg
     2821 cagaaaaaga agggttgatt gccgaactaa agccaaaaca tgaacggcaa aatttcttag
     2881 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaatg
     2941 agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
     3001 gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac ctaaagatcg
     3061 gtgaccctaa gcagttaaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
     3121 taaatcgtga tcagttaaac gatacgtggt tagtttcgat ggaagggtta aaacaccatg
     3181 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
     3241 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
     3301 aattaatggg cgcagttgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
     3361 ctaatgattg gcaagtgcca aaagaataca aagtcattta tgaagaagtc gcaaaagaag
     3421 aaaagatcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
     3481 cgaaccaatt ggtttccgct cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta
     3541 acgattcaat agaagctatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
     3601 aagcttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
     3661 atattaatgc tcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
     3721 atcatgaaca tgaatttgta cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg
     3781 aaatctacca acaagagatc agaaagcaaa aacgtgccat gttgtttcaa caaggtagac
     3841 agcaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca
     3901 caagcgaagg cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag
     3961 atccaagatc gattgttttc aggagaaact gtgatcacgt atggttttgg tcatttagtc
     4021 gagttagatt cgcccgatat gtacgatgaa aattggaaac aatggtcatt agagcatttg
     4081 cctattttcc ctacacatta tcactatcat gttcctaaag ataagaaaaa gcaattcaag
     4141 gtggtgaaac agcaacttca atcggccgac accattatta ttgcgacaga tagcgatcgt
     4201 gaaggggaac tgatagcttg gacgatcatt caacaagcag gcgccgatca tggaaagata
     4261 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccagcag
     4321 ttacgagatg cagaagagac ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc
     4381 gattggttga tcggcatgaa cggtagcccg ctgtatagtt tactcttgca gcaaaaaggc
     4441 ataccaggaa gtttttcctt aggcagagtc caaaccccaa cgctttacat gatttatcaa
     4501 ttacaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta
     4561 atcgctcaaa atggcgcatt tgatgcgaag cttgatccga atgaaaccca agcgacgcaa
     4621 gaagcatttg aagattattt aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg
     4681 atccaccaag ttgaaaccga gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc
     4741 agccttcaat ctaaaatgaa tcagctcatg aaagctagtg ccaaagatac tttggaagcc
     4801 atgcaagggt tgtatgaagg gaaatatcta agctacccac gaacagatac cccttatatt
     4861 acagaagggg agtatgcgta tctgttggat cacttagacg aatacaaaca ctttttaaag
     4921 gccgaagcca ttcctactcc tattcataca ccgaatagtc gatatgtgaa taataagaaa
     4981 gtccaggaac attacgcgat cattcctaca aagacggtca tgacggcagc ggcttttgaa
     5041 caattaagtc cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg
     5101 tttgcggaaa agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag
     5161 ctaaagacag tcggaaaagt accagtagat ctaggttgga aaaagctgtt tggcaaagag
     5221 agcgacagaa aagaaaagga agaagaaccg ctacttccta aagtgacaaa aggagaagcg
     5281 gtgacggtag atctacaagt ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa
     5341 ggtacgttga tcacagcgat gaaaacagct ggaaaaaccg tggatagcga agaagcacaa
     5401 tccatattga aagaagtaga aggaatcggt acagaagcaa cacgagcaaa tattattgaa
     5461 actttgaaac aaaaggaata tatcaaggta gaaaaaaata agcttgtggt gacaaataaa
     5521 gggattttac tttgccaagc cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc
     5581 gccaaatggg aaagttactt attaaagatt ggtgagcaga aaggaacaca agctactttc
     5641 ttagctaata tccaaaaatt tgtctctcac ttgttggaag tcgtaccggg acaaatccag
     5701 tcaacagatt ttggatcaac gttacaagag gtgaaagcag ccagtgaaaa acaggaagca
     5761 acgcgtcatt taggtgtttg tccaaaatgt cgggaacaag aagtcttgct ttatcaaaat
     5821 gtggcggcat gtacgtctga agcctgtgat tttaaattat ggacaacgat tgccaaaaag
     5881 aaactcacag ccacacaact gaaagagatc attcaaaacg gccgaacaag ccaatcagtc
     5941 agaggactaa aaggtcagaa aggttcgttt gaagcaacga tcgttttaaa agaagacttt
     6001 acaacaggct ttgaattttc tgaaaagaaa aaaacgaact ataagaaaag aacacgccgc
     6061 actacaaaat aatgaagggg ttatcctatg cgaaatacaa cacacaaaca acaagaaatc
     6121 gaaaatcaaa aagcaaaaat tcgtttagaa caagagaaat tacgtgcaaa taaaagaaga
     6181 ctgcaacaat taaagaacca gcagcgcatt caaaaagaaa tcgaattaga tcgatagacg
     6241 ttaatcttgt tacaaataaa ctactatgat atatttgatg tagtgtattt gtaacgaagg
     6301 gaggggaagt aaatgcaaga taaactgaaa gaattgttgg ctattcgcga tggaaattta
     6361 tctatgaagg aagccaggaa atttggaatc gcagcaacca ctgtacaaag attggtgaat
     6421 caaggaaaat tgatcaaagt tgatcgtggt tattatgtag aggatggtca tggaatagat
     6481 gatttatttt ggcttcagca acgattttcc aggggaatct tctctcatga aacgacttta
     6541 gatatttacc agctttctac gaatatgccg aaattcattc acttaacttt tccccatggc
     6601 tataacgttg atcgttcaat tacaaaagag cagcagttac aatttcattt cgtgaagaaa
     6661 gaagtttatg aactaggaaa agtagaggga acttcctttc aaggaaatcc tattactatg
     6721 tatgataaag agcgtacact ttgtgatatt tggaatccga gatatgaatt tgaatatgaa
     6781 atgaaattag aagcattaaa agagtacatg gaagacccat tgagagatcc aagtaaatta
     6841 agagattaca tgacaaaact acatgtagaa aaagaaatga aataccacat gcaaagctta
     6901 tattgatgga ggaaaaagat gacaccgaca caatttgcag caaaaacaag aaatttagca
     6961 aagagtacag gattaaatgc acagttagtg caacggcatt ttatgatgga tcgtctaatt
     7021 gaacaaattt ctgaatcaaa gtacaaaaat gacttcattt tgaaaggcgg ctttttatta
     7081 ggatcaaaat atggaatcga tagaaggaca acaattgata tcgatacgac atttagaaat
     7141 agcaaattaa ctgaagaaaa attgaagagc atttttaatg atttaactag aaaacccaca
     7201 aaagaaggca ttgttttctc tattcaagga atgaaggaaa caagagaagc cgatttttat
     7261 cctggatttc aaattaaatt ggtggctaat ttagagaaag tacgtgtacc gtttaaaatg
     7321 gacgtgacaa caggagattc tatttatcct gaagtagata cacactatca ccaattaatg
     7381 tttgaagata aaaaagtgga gattccagct tatccaacag aacaaattat tgcagaaaag
     7441 ctaagtgcta cgttcagctt tggcacagat aattcgagag caaaagatta ctacgactta
     7501 tttaccattc cgaaactaga aaatatcgat acgaaagagt tatacaaaag tgttcgaaat
     7561 acatttacaa aacgtgggga tacaaaaccg ttgtcctttt attatgaaag agaaatggat
     7621 aagatcagag gaaatgacta tttggaaaag cagtgggaaa aatatcgctc agttaacacg
     7681 tttgcgaatt ccatttcatt tacagataca gtcaacgaaa ttgatcacct aatgaaaagt
     7741 gtgatcaaaa ttgaaaatca agaaagaaaa cggcagttta aaagatcaca acaaatgaat
     7801 caagaaatgg agagataaag atgattaata atgtgacatt agtaggacgc ttaaccaaag
     7861 atcccgattt acgttatact gcaagtggca cagcagtagc gacttttacc ctagccgtca
     7921 accgaaattt cgcgaatcaa aacggcaatc gagaagccga ttttattaac tgcgtgattt
     7981 ggcgcaaacc agcagaaaca atggcgacat tagctaaaaa aggcggcttg atcggtgttg
     8041 tcggacggat tcaaacacga acttatgaca accagcaagg acaacgtgtg tatgtgactg
     8101 aagttgtagc agataacttt caactattag aaagtaaagc agcgacagag agccgtgcgc
     8161 acgctgatca atcaaccacc agtccaagcc ccactacctt tgaacaacgg gatacaaaca
     8221 atggattgga cgcttcacaa aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8281 atctaccatt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8341 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8401 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     8461 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8521 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8581 cagacagcaa gactattata gcataattga aatattcaaa tgattggatt tttgctataa
     8641 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8701 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8761 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8821 tattgccgtc acaggtcact tcaataaacg aaaccagcta gttattgcta gcatgtatgt
     8881 tagaaatccc gatcagttta cgcgattgat gggcatataa aaaaagaaga gttgcaatcc
     8941 taatgtagga ctgcagctct ttttgtttta catagaaaca agaaaaatga aaaggccttt
     9001 ttaggaaggc gcgagcgcct tctcaattga aaaacctctc tcactccaac cgccaccgcc
     9061 gttcgagaag aaaggaagtg aacgttgcac gttcactaag ataaagggtc ttgcaaagca
     9121 agacaaaata gaaaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     9181 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     9241 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgattcatt ctggccaaca
     9301 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     9361 aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     9421 tcatgcacca catactgtcg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta
     9481 tcgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgattatat
     9541 tttgattgat gagaaaggaa agaaacgttg atgaaaacac aacacaaatt attgactgta
     9601 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagct
     9661 ttacttattc cgcggtactg gacgacagaa acccctttag tagaggatgc accgactatg
     9721 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacttggaa
     9781 acttatgaaa acgaatattg gttaaccgaa ttagagaact aaaaaggaga aaaaaatatg
     9841 gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg
     9901 gaggatatga cattcctata caatgagaca cgtgttccaa aaaaacatta taaggaaaga
     9961 ttgtcggtag ctgtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg
    10021 tattatcgaa cactggaaga actgaaaaag caaaatgcaa aatggttttt ccaagcctta
    10081 ctatgcttgg aagtaggcgt caaaccaagc acaatcaaac cttctgaata ccaagcttta
    10141 gaactaacgt atgcgaaatt tatcgaaacg aaaaaagcta agactgtttc aagtgaatgg
    10201 ttagattatt ttgaaaatat caataagtat ggcgcatgtt acatcatgaa gtaatcagac
    10261 ggattcaaaa agactttagt tgtatggaag aaatgaagta ctttgttcaa aatgttgaag
    10321 aacagttata tttggaaagc gaaacgagtc aaacaaaggt cacagtcagc cataatcgtt
    10381 tagatgtgta taaacgtcgt cctgaatcat taaaaaaagg agcggagctt ctacgactag
    10441 aaacagaaaa atacgtcatg aatgtttacc tagaaaaaag tgagttggtc gaaaatttta
    10501 tggactagcc gagaacaccc gtgattttaa tcatgggatg aatcggattc agttgtgacc
    10561 tagctttagc taggttaaac aactgacaaa tataaactaa ataatagtga aatgtcttgc
    10621 aataattgat tttgtagaaa tacatatatt aaaataattg cgaggtgttt ttgtatgtca
    10681 gaaattcatt atggacgggg ttatgtctat tctattcaat atcatcttgt ttggtgtgta
    10741 aaatatcgac atgatgtttt gtatggtcag gtagatattg atgtcaaaca gtttttgaaa
    10801 caaattgctg atgataataa tataaaaatt atagaaatag aatcagataa agaccatatt
    10861 catttactca ttgaatgcac cccacaacac tatattccta gtattgttaa agtatttaaa
    10921 ggcgcatccg ctagactgct gtttaaaaaa catcctgaac ttaaacaact cctttggggt
    10981 ggacatcttt ggaatccaag ctactttgta gctactgtta gcgaaaatac agaagaacaa
    11041 attagaaaat atatacaaaa tcagaaaaag aaatgaggtg aatccgtatg ctacaacata
    11101 gggcttataa atttagaatc tatcctaatc aagaacaaga aatattaatg actaaaacta
    11161 ttggctgttc aagatttgtc tataattatt ttttgaattt gtggaatcat gaatatacga
    11221 ctactggaaa aggattatct tataattctt gttctgccat gcttccacaa atgaaaagaa
    11281 atgcagagac aatttggtta aaagaagttg acagtattgc gattcaatcc tcattgaaaa
    11341 atctttctga tgctttttct cgcttcttta aaaagcaaaa taaacgacca caatttaaaa
    11401 gtaaaaaaca tcctatacaa agttatacaa ctaaaaatgt gaataatagt atcgaaatta
    11461 gaaacaattt cgtaaaacta cctaagttag gatttgttaa atttgcgaaa agtcaagagc
    11521 ccaaagggcg aatcttaaat gctacgattc gaaaaaattc gagtggtaaa ttttttgttt
    11581 ccatcctgtg tgaagaagaa atagttgagt ttcctaaatc taattccgct attggaatcg
    11641 accttggtat aactgatttt gcaatccttt ctgatggtca aaagattgat aacaataaat
    11701 tcacgtcaaa aatggcaaaa aaactcaaac gtgaacaacg taagttgtca aaacgtgcat
    11761 tactagctaa aaagaaaggc gtcaatctct ttgaagctaa aaattatcaa aaacaaaaac
    11821 gaaaagtagc acgactacat gaaaaagtaa tgaatcaacg caatgacttt ttaaacaagt
    11881 tgagcacaga gattatcaaa aaccacgata ttatctgtat agaagactta aatacaaaag
    11941 gtatgttgcg taatcataaa ctagcaaaaa gcatttctga tgtgtcttgg tctagttttg
    12001 tgactaagtt acaatacaaa gctaactggt acggtcggaa aatcatcaaa gtagataaat
    12061 ggtttccatc tagtcaaatc tgttcaaatt gtggacacaa agacggcaag aaatctctag
    12121 aaattcgaga atggacttgt cctgtttgtc atgctcatca tgaccgagat atcaatgcta
    12181 gtatcaatat tttgaccgaa ggtctaagat tacatgtcat gggattagct tagatccaat
    12241 catcaaaaac cgtaggaact acggggatag cttggtaaat aagagaaacc gctgtgagta
    12301 aagaaatacg cttgcaagta tgctctattc ccaagaatct cgtgacttta gtcatgagag
    12361 gttcaaggat tcatggaatg ttaatctaat ttaatagaaa aggataatta agtatatacg
    12421 ttttttctta tgtatgttat actatgtata tacagaaaga ggtgtctact atggaaatgt
    12481 tacctataaa aaaatgggga aatagtaatg gccttagatt gccaaagtat attatggaat
    12541 atcttggaat tcatacagaa gacaaagtga agataataca agaagaagta aacggtcaga
    12601 agcggctaat tatcgaagcg gctaatatag aaaatgaatt aacaattgaa cagttatttg
    12661 aaaattacac tgatgaaaga aatcatgtga cgattcaaaa tttaggtgaa gcagtaggta
    12721 atgaaaaatg gtgagaaaac ctagacaagg cgatattcta ttattgaata cagcacctcg
    12781 atctggacac gaacagacag gtaaaagacc ttatattgtt ttaagtcatg atattattgc
    12841 ggattatagt aacgttgtta cagttgctcc aatttctagt acaactagaa actatccgct
    12901 atatgtaaat ataaactcag aatatgaaat gaagacaaca ggaaaagtat tattagatca
    12961 actaacgaca attgattatg aagccagaga gtgtattttt ttggaaaaag cgcatgatag
    13021 tttagttgag gagttattga tgaaagttag aacagttttt caaaaagtaa gcaaataaaa
    13081 tagaaacagc agcatgatca actattcgtg attgaccatg ctgtcttttt ttattctata
    13141 tacggtttgt ctcgtaacat tgtagtcttt tgcaatctta gcgatcgcaa cacctttttt
    13201 tagatcctca acgatgtttt tataaacaag acgacgttgg ggatcttttg cattggcact
    13261 gtatagctta ggacgtcctt tataaacacc gttcgctttt gcaatcttaa tgccttgtgc
    13321 ctgacgtcgt ttagattcgg tacgttcttg ttcagcgatc atggccaaaa tttgaatgat
    13381 caaatcttta atgaatttat ccaggagagg attgccaatt gcttcggcca tgatcggtag
    13441 acttgtgatg atcagctgca catttttctt cttcaagtaa ttgactgaag cgatgatctc
    13501 gtcgtaattt cttccaagtc gatcaatagc ttccacaata aaaatatctt gatctcggac
    13561 gaaatccagt gcttcttgaa aaacaggtcg atgagtaatc gttgcgccag attgtttctc
    13621 aatgaaaatt ctctctgcac cgaatttttt catttcttca atctgccgtt gttcattttg
    13681 atcgatggat gagacacgta tgtatgcaac tctcaaaatt tttacctact ttctgcaact
    13741 atttaaaaaa caccctaatc atacacgaga aaaccagatt tttcaatgtc gcggtcgtgt
    13801 ctagttaggg ta
//
LOCUS       DAFBQW010000068        13625 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFBQW010000068 DAFBQW010000000
VERSION     DAFBQW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.071"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1539)
                     /locus_tag="LK889_002334"
     CDS             complement(<1..1539)
                     /locus_tag="LK889_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5096538.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQ"
     gene            complement(1532..2983)
                     /locus_tag="LK889_002335"
     CDS             complement(1532..2983)
                     /locus_tag="LK889_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5096539.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(2997..4013)
                     /locus_tag="LK889_002336"
     CDS             complement(2997..4013)
                     /locus_tag="LK889_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5096540.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4233..5321
                     /locus_tag="LK889_002337"
     CDS             4233..5321
                     /locus_tag="LK889_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBK5096541.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5375..6901
                     /locus_tag="LK889_002338"
     CDS             5375..6901
                     /locus_tag="LK889_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK5096542.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            6903..8108
                     /locus_tag="LK889_002339"
     CDS             6903..8108
                     /locus_tag="LK889_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5096543.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            8365..9342
                     /gene="guaC"
                     /locus_tag="LK889_002340"
     CDS             8365..9342
                     /gene="guaC"
                     /locus_tag="LK889_002340"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK5096544.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            9606..11030
                     /locus_tag="LK889_002341"
     CDS             9606..11030
                     /locus_tag="LK889_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK5096545.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            11491..12600
                     /gene="pdhA"
                     /locus_tag="LK889_002342"
     CDS             11491..12600
                     /gene="pdhA"
                     /locus_tag="LK889_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBK5096546.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            12603..13580
                     /locus_tag="LK889_002343"
     CDS             12603..13580
                     /locus_tag="LK889_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBK5096547.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         4347 a         2341 c         2949 g         3988 t
ORIGIN      
        1 ctgtgtccga tattcttttg gagaaatccc ttgttttttc ttaaataaat aactgaaata
       61 gttagggtca ttataaccaa tttcaaaagc aatatctgaa atacgatagt ctgattgctg
      121 caataatttt tttgctaatt cgattctttg ttctgtcaga taatcgatga atgtttggtt
      181 aaacgattga gaaaaaatcg tactgaaatg agagggactt aatgcaactt gttgagcgac
      241 ccaagttaag gacatatctg gatcggtaaa attttctttg ataaaatcca atgcatggtt
      301 caaaattggc tgatatttgt tccttgaagg ctgtgagggc agagcagtta agaaagaaat
      361 gaacatataa actgtcgaac ggtaaagcga gtcgtcggcg ctgattgcta agcgttgact
      421 ggtggatgtg caatgaacca tttcttgcgg gattttcttt tctttcaggc agacatgctg
      481 gagtagctta gtcaagacaa ataaacgata taatcgtgtt cgagttggtg tgccttgtgg
      541 tttcgtcaag tgttcgatgt acttagatac ttcgtttttc gataatgaag acaaatcaag
      601 tgagaggtca aagtaaaaga cagaatcaac tggaagtttc gcttcttgaa tcaaatcctc
      661 atagctgata atttgttctt taagggaact ggcggtttca agtatcatct gcgtgatctc
      721 aaaagatgca ggtaattcgc tgattctttc tgcgacagga ccaatggcga cagaaaaatt
      781 atttttttgt cccttttcta attcatggac caacgtttgg gctacctgat aactggtctc
      841 gatcacattt tcttcaagag aatcaactat taaaaatttg atgaatcgag aggaaacagc
      901 agagaagatc acttgttgat tctccctaaa taaaaacttc aaataatcac tgagatgagc
      961 gtaatcttca aagtctgtac tgacttggtt tgctgctaat aatacggtca tttttttccc
     1021 tgctaaagag cgattgaaag attcagcaag tttcaatgtt tcactgatcg taagtgagcc
     1081 tttgaacaaa ccgttcaaga aatgattttt tttcaattcg aaaacaagat cagaagaagt
     1141 gtgcgtttct ttttgctgca aatctaaagc agttactgct ttttctattg cttgtttcaa
     1201 ttcgctactt ttaatcggtt taagcagata atcatctgcc tgtacctgga ttgctccttt
     1261 catataatcg aaatcgtcga atccactaat aaatatgaga cgagtccaag gaagtagttt
     1321 ccttgcttct tttgaaaaag tcaacccgtc catgaaaggc atttggatat cagtcagaat
     1381 gatgtcgggc tgtaagtcga taattgaaga cagtgccatc tcaccatctc cagcttcgcc
     1441 gacataagtg attggataat aagaaccaag gacagttatt tgttcccgca agttattgcg
     1501 aatgaggtat tcgtcttcga tgataaaaac tttatacatg tttgtttcct ccaattgttt
     1561 tgggagatag agggatcatc agtgtgacag ttgttccttc tcggtatttg ctgtcaatcg
     1621 tgataggttt ttgatcattt ccgtaataaa gaaggagacg cttgtttacg ttgtaaagtc
     1681 cgtagccgat actgaaatct gaatcgatcc ctttggctaa ctctttacgt atttctgcca
     1741 gtttttctgc agggatgcca atcccgttgt ctataacttt gaattggatc atctcatcaa
     1801 ttttgcatgt ggtaatacga ataaatcctt tgtgacggac gtacttcgtt ccatggtata
     1861 cagcattttc aatcaatggc tgcaagatca tttttaaaat agaaacagga aaaagtgttt
     1921 ctggtatatc gatcttatag tcaagcatat ggccgtatct tatttttaag atgactagat
     1981 aatcattgat atggctaatt tctttttcaa gaggaatcca gtcttgtcct ttgcttaaag
     2041 acgtacgaaa aaagccggaa agtgcatggg tcatagtcag agcttcttca aagcagtctt
     2101 gttcgatcaa agaaataatg gcatccaaac tgttataaat aaaatgggga gtaatctgag
     2161 cttgcaatac acgaacttca ctttgtgcta aatggtattg tttcaaagcg ttctcccgta
     2221 aagactggtt tagatcttcc gccattttat tcatgctagc ggtaagtgtt tgtaattcca
     2281 gtgtttccgg cagggttaaa cgaaagccaa ggttccctga agaaagttgt tctgacatct
     2341 cgatcagctg attgatcggt tcttggacct ttctctctaa aaagcgacga ttctttttcg
     2401 catagaaaaa aatggtacta agaactccaa gttccactat tgttaataga atgagcgagc
     2461 ggacaagtgt cgtatttttt ttgcttgcta agtcgatctc cacacgaaca aaatcttgta
     2521 agatatctga cagcagttga gtgacagaat ctacctgatt gatgatctct tcattggtat
     2581 ccataaaatc ttttgccaag atattattga tcagctcgtt ttgataatct tccagtgtat
     2641 cgatcgtacg tagcgataca tctaacgtac taatttcttt aagtgtattt gtattatttt
     2701 tgatctctcg aatatcattc ctaagttctt ctaggatatt ttcctcattg aactcatcta
     2761 cggttacttg gccagaaatc aagtcccaca tactttccaa aactttttga tcaaccttca
     2821 aagcaattgc atctgctttt tggatatttg cgatcgtctg ttgataaatc aaaagctgtt
     2881 gcgaatataa cagtatgctc agtagcaaag ggataacagc aaatagaagc atggctttgt
     2941 ttgcttgggc aaaaatctgt tttatgctta aattttttgt catgatactt ctccagtcaa
     3001 taatttctgg ttgggatgtt agctatattc tgctgggaaa agacagtctc ggaaataaaa
     3061 atatctttag gtaatgtttt tcctgtttga atatttccaa aataacgagt aacggtattg
     3121 gctacatacc agccgacatc aggtttacac tcgactacac aattgacgat tcctgagcgt
     3181 agcttctcga tcatatcttc ttgtccatca atagttacaa tgatcaaatc ttctccaggt
     3241 ttgattgatg tttcttttat cgcagctaaa gcaccgtgag tcatttcatc actatggctg
     3301 tataatacat cgatttcgga taaatcattt gtttctaaat atcgtttcat ctggtctttt
     3361 cctttcatcc gaataaaatc accgacaatt cggtgaacaa tagtcatcgg aggtttatgg
     3421 tcgatggttc catctttttt tagggttggt ttacgattga ttgattccaa aaatccttcg
     3481 gagcgtagct tggtcggtgt ggagttctct gaaccagcaa gttctaggat tctgatttcc
     3541 tgtttctttg aatttgtgaa gtgattgctg acaaataaac ccgcccgatt tccttctgct
     3601 ttgaaactgg ggccaatgtg ggtgacgaaa agctcgggat cattcgtttg aatatgtctg
     3661 tcgacaagta tgacagggat ccctgctcgc tttgcttctt ttaatacaga gtcccacccg
     3721 gattcttgca aaggagtaaa aatgatcata tccactttat aggcaatgaa cgtccgtata
     3781 tcttggattt gccgttcctg attcatgtag ccatttcggt agagaacttg gtaatcttct
     3841 ttttcaagcg cagtgataat tgattcagta tgctttttcc gccagctact ctccgttcct
     3901 gattgcgaaa aaccaacgac tagtgggtca ggtgcttcct ttgtattagg tgtacacccc
     3961 atcatttggt aagcgattac aacaagggct aaaaaaaata aaacacgttt cactttaaat
     4021 cactccatct ggatttgtta gtactatctt atcagataaa agggaaaatc aaagtcaaaa
     4081 ggaatcaatc gaaaaaaatc caagaaactt aaaaaaatcc aagacaaaaa aagtacacct
     4141 aaaaaattta aagtaaatcc cataaaaaaa tcaatgttaa cgctttcttt attagctata
     4201 ctcggtgtat caattgaaat gggggataaa aaatgaaaaa caaatttaga aagattgttg
     4261 ggactatcgc tattttgacc gcaagcattc tattaagtgc gtgtggcaat tcaggatctg
     4321 cagatgatag tacggggtat gtaggaatcg ctatgccgac caaatcagca gaaagatgga
     4381 tcgctgatgg gaacaacatg gtctcggaat tagaaaaact gggttataaa acagatcttc
     4441 aatatggaga agataaagta gaaaatcaag tagcacaaat agaaaatatg attacaaaag
     4501 gggtagatac acttgtgatt gcttctatcg atggttcggc tttaactgat gtgttagcta
     4561 aagcaaaaga agcagatatc aaggtgatcg cctatgatcg tttactgatg aattcagaaa
     4621 atgtcgatta ttacgcgact tttgataatt ttggagtagg tgtatcgcaa gcagcttata
     4681 ttgaagagca cttaggattg aaagaaggaa aaggaccatt cacgattgaa ttatttggtg
     4741 gctcgcctga cgacaacaac gcactaatca attacaacgg agtgatgtct gttttacaac
     4801 catatatgga caacggtcag ttggtcgtgc catctggaca gacgagtttc agtcaaatcg
     4861 ctacgcttcg ctgggatggt tctacggcgc aagcaaggat ggataatctg ttaagcgcaa
     4921 attatactga ccaaacattg gatgctgttt tgtcgccata tgatccaata agtttaggaa
     4981 tcatttcgtc attaaaaggc gtggggtatg ggagtgaaag taaaccttta cctgtgatta
     5041 ctggacaaga tgcgacagtt gctggcgtta aatcgatcat tgccggggaa caaacacaaa
     5101 cgatctttaa agacacacgt atcttagcaa aaaatacgat tgaaatgatc aaagcaatca
     5161 gtgatggaga agaggttccg gtcaatgata ctgaaactta tgataatggg gtgaaaactg
     5221 tccctacgta tttagcaaat acagtatctg tcgataaaga taattatcaa gcagagctga
     5281 tcgacaccga ttattataaa gaatccgatc tgaaaaatta agaggaacca aacaaggaac
     5341 taaagagatc taagtcagag agaggataag aattatggcg gattgtattt taaaaatggc
     5401 agatatcacc aaaaaatttt ctggaatccg agcgttagat tgcgtgaatc tgatgattga
     5461 acgtggggaa atccatgcat tgtgcggaga aaacggagcg ggaaaatcaa cattgatgaa
     5521 tgtcttgagc ggcttgtatc catatggctc ctatgacggg gaaatctttt atgatggaaa
     5581 gctgtgtcaa tttaaagatt tgaaagatag tgaagaaaaa ggcatcgtca tcattcatca
     5641 agaattagca ctaagtcctt atctttcaat aaaagagaat atttttctcg gaaatgaaca
     5701 agcagaaaga ggcgtgattg attgggaact gacagaaaag aaaacatatg aactgctgaa
     5761 taaagtcggt ttgtctatcg acccgaatct tctagtatcc cagattggcg tagggcaaca
     5821 gcaactagtt gaaatagcta aagcattatc aaaatccgtc cgtctgttga ttttggatga
     5881 gcctaccgct gcattgaatg aagaagaaag cgcgaatttg ctgagattga tccgagagtt
     5941 gaaagagcaa ggtgtaacct ccatcattat ttcacataag ctgaatgaaa tcgtggatat
     6001 cgcagatcga atcaccattt tacgggatgg acagacgatt gagacactag aaaatgaaat
     6061 gatcagcgaa gagcatatta ttcgcggaat ggtgggcaga gatttagttg atcgttatcc
     6121 tgaaagaaaa ccggatattg gagaagttta ttttaaagta gaagactgga cggtctatca
     6181 ccctcttgat cacgagcgaa tcataaacga ccacatcaac ttgcatttga aaaaaggaga
     6241 gattgtagga atagctggac tgatgggagc gggcagaact gaattcgcta tgagtctatt
     6301 tggacattct tatggaagca agatatccgg acgtatatta aaggaagggg cagagatatc
     6361 agttaagacc gttccttcag ccattgcaaa cggactcgct tatgtctcgg aggaccgaaa
     6421 atctttagga ctgaatctgc tgatggatat tcgggaaaac gtgacgctga gcagtttgga
     6481 taaaataagt caaaaaggag tattagataa agaaaaagaa gtaactgagg cagaaaattt
     6541 ccggaaaaag atgcgtataa aaacaaactc catccatcaa attgtcagta gtcttagtgg
     6601 tgggaatcaa caaaaagtcg ttttatccaa gtggctgatg acacaacccg atattctttt
     6661 tttggatgag ccaacccgtg ggatagatgt tggggcaaaa tatgagatct acacgattat
     6721 cgaagaaatg gcagcaaatg gcaaagctgt ctgtcttatc tcttctgaac ttcctgaaat
     6781 attagggatg tgtgatcgaa tctatacgat gaacgaagga cgtattacag gagaagtctc
     6841 gcgagaagat gcagatcagg aagtcttgat gaaattaatg acgaaagaag aggtgggtta
     6901 agttggaaca ggcaaaaaat cttacggata aaaaagagaa aaaactaaat ttgaaaagta
     6961 gagcagctga tctttttggt cgctacagca tggtgatcat tttagcggga ctacttattg
     7021 cgtttcaatt gatgacggat ggtattttct ggcgtccatt gaatataact aaccttgttc
     7081 tgcaaaatag ccatatatta gtactagctg cagggatgtt attagttgtg ctcttaggtc
     7141 atgtggattt gtcggtaggg tcagtgatgg cgtttgttgg tgcaattgcg ggcatgatga
     7201 tggtcaatcg ccaaatgaac ccgtggatga cagttatcgc atgtcttgca gtaggcgggc
     7261 tgatcggcgc atggcaaggt ttttgggtag cttatgcagg gataccggcg tttatcgtga
     7321 ctttagcagg attgctgatg tttcgtggac tgacacaagt tgttctagga ggccaatctc
     7381 tagcaccttt tccagctgtt ttccaaaaaa tgtctacggg ttatttacca gacttaagcg
     7441 aaggaagcag ccttcacttc ttgacattga ttttaggaat gatcatcacg atcgtgttag
     7501 ttgttggtca atggcgaaca agaaatcgtc ggaaaaatca tttatttgaa gtggaatctc
     7561 caacagcttt cttggtaaag gcggtattta cagcagtcgt tgtactagga ataacttata
     7621 tctttgcctc ttatcaaggg tatccgatta ttttgatcat tcttggtttg atcgttgcag
     7681 catatgggtt tttgacaaat aaaacagtag caggtcgaca gatttatgcg acgggcggaa
     7741 atcgaaaagc ggctgaacta tcagggatca aaacaaagcg aatcacgttc tgggtattcg
     7801 tcaacatggg agtcatggca gctttagctg gattagtttt agctgctcga ttgaatgcgg
     7861 caacacctca agcaggtaca tcgatggaac tggatgcaat ggcggcagta tattttggtg
     7921 gtgcttcgac ttccggcggc attggaacaa tcatgggtgc gattgtcggt ggcttggtca
     7981 tgggtgtatt gaacaatggc atgtcgattt taggcgtcgg ggtagactgg cagcaggcaa
     8041 tcaaaggatt gatcttgctt ttagccgttg tgctagactt gtataataag aaaaaacgaa
     8101 caacgtagga gaacgccggt tattcagaaa aaagactgtg acaagacatt tgtcacagtc
     8161 tttttttcgc taaaagcgaa cattcattgt ttcgtttgta tagactaaaa ataatatatg
     8221 tttttttacc attcgtataa attctctttt tgctcttgat ttttttgctt aattgagtta
     8281 aattacatag ggcgctaaaa aaagccttga ttaaattaat aatgttcgtg ttttgctgga
     8341 tgtgaacatc aggaggataa tgtaatgaaa gtatttgatt acgaagatat tcaattaatt
     8401 cctaataaat gtatcgtaaa tagccgttct gaatgtgata caacggtcac attaggtaaa
     8461 catacattca aaatgccagt agtgcctgcc aacatgcaga cgattattga tgaaacaatc
     8521 gcagaattct tagcggaaaa tggttatttc tatatcatgc atcggtttga tgaagcagct
     8581 cgaattccgt ttatcaaaaa aatgaaaaag cgtggattga ttacctcgat cagtgttggc
     8641 gtcaagaaag aggaatattc ctttatagag aagttggcag aagaatcgct aaatcctgat
     8701 tatatcacta tcgacatcgc gcatggacat gctaactcag ttattgatat gattcagcat
     8761 attaaaaaat atttgcctga aacttttgtg attgccggta atgttggtac accggaagcc
     8821 gtccgtgaat tggaaaatgc gggcgcagat gcaacaaaag tcgggattgg tccaggaaaa
     8881 gtatgtatta caaaaatcaa gacaggcttt gggactggag gctggcaact ggcagctctt
     8941 agatggtgtg cgaaagcagc tcgcaagccg atcatcgctg acggtgggat cagaacacat
     9001 ggcgatattg caaaatctgt ccgttttggt gcgacaatgg tcatgattgg ttcattgttt
     9061 gccggtcacg aggaatctcc tggcgaaaca aaagtcgaaa atggaatcgt gtataaagaa
     9121 tactttggtt ctgcttcaga attccaaaaa ggcgaaaaac gaaatgttga aggaaagaaa
     9181 atctggatcc aacacaaagg atctttgaaa gatacactag tggaaatgca acaagacctt
     9241 caatcatcta tttcttatgc tggaggaaga gatctggaag ccattcgaaa agttgactat
     9301 gtcatcgtga aaaattcgat ttttaatgga gacacaatct aaaaaataaa taaaatatta
     9361 ggatgttgtg aaaaagttgt cctgcattaa gtaataagaa cagccaaaca aaaatagttc
     9421 tttatatgtt ttgtttggct ggaattatta tcgcagatac aagcttttga acatcattat
     9481 tcagaataag ggactgtgat aagatttttg tcacagtccc ttatgttttg caaaaaaagt
     9541 ttttcttttt tctcgtaaat tcggtaaaat caaaagaaaa aatagacaat agaaaagaga
     9601 agcttatgtg ggaaaaagta gagaaaaaac aaggaccgat ttatcgtcaa attatggaac
     9661 agattatgag agaaatcgaa aacggaagga tcaatcctgg aagtcaactt gaatcagaac
     9721 gaaaactagg gcaacttttt ggtgtgaatc gttccaccgt tgtccatgca ttggaagagc
     9781 ttagggagct tggggtacta acgagcaaac gtggcagcgg atggtttgtt aatcagactg
     9841 aatgggggaa attcgctgtt ccaagaatcg attggcggca aatgatctct cctcgttatg
     9901 agcagacgga ctggtatgag caaaaaataa gagaagcaaa gcagatccaa aaaacatcgt
     9961 ttcttgattt atacgctagc gagatgccag atgagctttt gccgaatttt gagtttcctt
    10021 cttattctct tgaagaaatc ttagcggaag aaaaagaaat ggatctttta ggctatactc
    10081 cgttacgtaa caaaatcaaa cggtatttgg aaagtaaatt tgaattggca ttttccccca
    10141 accagcttct tgtgactggt ggcggtcagc aagcaatctt tttgatttta caaacgattc
    10201 tttcagctgg agaggggata gctgtagagt caccttcttt tttttaccga ttacctcttt
    10261 tcaaagcttc aggaacgcgt ttatttggga taccgatgga tgaagaaggg atcgacttag
    10321 aaaaactgaa agaatcgatc cataaaaata aattgaaagc agtgcttatc aacccgaatt
    10381 ttcaaaaccc tacaggaaaa gtgatgagtc aaaaacgacg agaaaagcta gtagaattgt
    10441 gtcgacagta tcggcttcca attattgaag acgatgtatt tacggatctt tcatttctaa
    10501 accaagaaga agtccttcct atacgaacga ttgatccgga taatgtgtta tacgtcggtt
    10561 cgttatctag ggttttagga aaaacaacga aaatcggttg ggtgatagga cctgagactt
    10621 ttatctcgca actagctcaa gcgcaagaaa tgatggagtt ttcgatgaat atccttacac
    10681 aaatcacagt ggctaatgta tttaatgaag caattgattt gaaaatgcaa gatgtaagaa
    10741 aaaagttgaa agaaaaaagc cgactgataa gcgcttgggg agattcacaa tcgtttttca
    10801 agcttcattc aattttaggt ggttactatg cctggattac ttgggatggg aaaaaactag
    10861 accccgaact agcagaaaaa gtagtagata tgggtcttgg aattgcacca agtctacttt
    10921 ttgggcaaga tataaatgga atcagaatca attacagtag gctggatgaa aaccaaatac
    10981 cgttgtttta ttcaaagatg aaaattctag aaaagtggtt aacagaataa tttcacaaag
    11041 taaatagatt aaagaatata acagcttgga tttaaatttc aagctgtttc ttttttttcg
    11101 gaaaaagaat acgatataaa gaaactaaga aaaaaccatt atttgtacgt ccgttcacga
    11161 tgtgtaaata aaataaatgt ttgcaacaaa actgtataac accaacgttt ttaaatagaa
    11221 acagatggtt gtaagtttga ttagaaaata gaaaaaatgc agatgaaata taaaacgctt
    11281 tccttattgt gattttattc actaatgctg ttaatatgcg gattggcata atttcctttc
    11341 atcgtgaaaa tttgtgcttt tagcttgtat ttctcataaa tccatgataa tttattaatg
    11401 aagacggtta aatagtgata cagttttatt cactgtttca atcgcagaat gaagagattt
    11461 tcttatagat gagagaggaa tgtgtgacaa atggcaaaca aaaaagcacc gatcgacttc
    11521 caagcactgc tagatgaagt caatgcagat ttcccagtat atcaagcact tgatcaagat
    11581 ggaaacgtag ttaacaaaga tttagtacct gatttatccg atgatgaact cgttgaatta
    11641 atgactcata tggtatggtc tcgtgtatta gaccaacgtt caacagcgct gaaccgccaa
    11701 ggacgcctag gcttttttgc accaactgct ggacaagagg caagtcagct ggctagtgct
    11761 tacgcatttg ataaagaaga tgttcttctt ccaggttatc gggatgtacc tcaactagta
    11821 aaacatggtt tacctttatc tcaagctttt ttatggtctc gaggacatgc tgcaggtaat
    11881 tcttatccgg aagaattgaa agctctacca ccacaaatta tcatcggtgc acaatatgta
    11941 caagcagccg gtgttgcatt aggtttgaag aaacgaaaca agaaaaatgt cgtcttcact
    12001 tatactggtg atggcggttc atcacaaggt gatttttacg aagcaatcaa tttcgctggc
    12061 gcttaccatg caaatgccgt attttatatc caaaacaacg ggtatgcgat ctctacacct
    12121 cgtgaaaaac aaactgcagc aaaaacactt gcacaaaaag ctgtcgcagc tgggattcct
    12181 ggtattcaag tcgatggaat ggatccgctt gcagtatata ctgtatctaa aatggcacgg
    12241 gaatgggcga tcagtggaaa tggaccagtc ctaatcgaaa cactgactta tcgttatgga
    12301 ccacatacac tatctggtga cgatccaacc cgctaccgta caaaagacac agatgacgat
    12361 tggcagaaaa aagatccatt gatccgcttc cgtaaatatt tgacggaaaa aggactttgg
    12421 tctgaagaaa aagaagaaca agtcatcgaa gcaactaaag aagaaatcaa agcagctatt
    12481 gctgaagccg acaaagtacc aaaacaaaag gtttcagatt tcttgaaaaa catgttcgaa
    12541 gtatctccac aatcaatcaa agaacaaatt gcaatttatg aagcgaagga gtcgaaataa
    12601 tcatggcaca aaaaacaatg atccaagcaa tcacagatgc cttagcactt gaactagaaa
    12661 atgacgagaa tgtcgttgtt tttggtgaag atgttgggaa aaacggagga gttttccggg
    12721 caactgaagg attgcaggaa aagttcggag aagaccgagt attcgatact cctttagctg
    12781 aatctggaat cgcaggattg agtttcggtc ttgcgttaga aggatttcgt ccagttcctg
    12841 aaatccaatt ctttggtttt attttcgaag caatggacga agtagtagct caaatggctc
    12901 gtacaagata tcgaatgagc ggcactagaa atctacccat cacaattcgt tcgccatttg
    12961 gtggcggtgt tcatacacca gaattgcatt cggataattt agaaggtttg atcgctcagt
    13021 ctcctggtat tcgagtagtg attccttcca acccttatga tgcaaaagga ctattgattt
    13081 cttctattag aagtaacgat ccggttgttt tcctagaaca catgaaacta tatcgctcat
    13141 tccgagaaga agtgccggat gaagcttacg aagttccttt ggataaagca gcagttactc
    13201 gtgagggaac agatgtatct attatcacat acggagcaat ggtgcgtgaa gcaatcaaag
    13261 cagcagacaa tcttgcaaaa gaaaatatat ctgttgaaat cattgatctt cgtacagttg
    13321 cgccattaga tgtagaaacg atcattcaat ccgtggaaaa aactggtcgc gtagttgttg
    13381 tccaagaagc acaacgacaa gcgggcgttg ccgcgcaagt agtttctgag atctcagaac
    13441 gcgctatcct ttcattagaa gccccaatcg gacgagtttc tgcacctgat acagtcttcc
    13501 catttggaca agcagaaaat gtctggttgc caaatgcaaa agatatcgaa gacaaagtaa
    13561 aagaaatcgc ggaattttaa gaaaagaagg gacgaaacaa agtcatggct tatcaattta
    13621 aattg
//
LOCUS       DAFBQW010000069        13443 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFBQW010000069 DAFBQW010000000
VERSION     DAFBQW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.072"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(77..149)
                     /locus_tag="LK889_002344"
     tRNA            complement(77..149)
                     /locus_tag="LK889_002344"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="LK889_002345"
     CDS             530..1099
                     /locus_tag="LK889_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBK5096548.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="LK889_002346"
     CDS             1086..1946
                     /locus_tag="LK889_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5096549.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="LK889_002347"
     CDS             2136..2840
                     /locus_tag="LK889_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5096550.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="LK889_002348"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="LK889_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBK5096551.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="LK889_002349"
     CDS             4677..5993
                     /locus_tag="LK889_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096552.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="LK889_002350"
     CDS             5994..6863
                     /locus_tag="LK889_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBK5096553.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="LK889_002351"
     CDS             6918..7727
                     /locus_tag="LK889_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5096554.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="LK889_002352"
     CDS             complement(7830..8459)
                     /locus_tag="LK889_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK5096555.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="LK889_002353"
     CDS             8629..9921
                     /locus_tag="LK889_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5096556.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="LK889_002354"
     CDS             complement(9997..10659)
                     /locus_tag="LK889_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5096557.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="LK889_002355"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="LK889_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK5096558.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="LK889_002356"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="LK889_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK5096559.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13141..>13443)
                     /locus_tag="LK889_002357"
     CDS             complement(13141..>13443)
                     /locus_tag="LK889_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5096560.1"
                     /translation="EAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLL
                     TYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT         4448 a         2320 c         2902 g         3773 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtgg ctctttgtca ataaggactg
    13081 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg
    13141 ttatcttaca ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga
    13201 tttaaatccg taggatactc gtttcattgt ctttatgtgg gtattcttcg cttctatttt
    13261 tccattggaa taaggataga tcattgcgtt ggtgatgcct tcttcatagg tcagaaggtt
    13321 ttgaagcttt tcccgaaagc tgtcatctaa cgtttcggga agttctgcca ataaggagaa
    13381 aaataagtca gggtctttgt ctcgaaaggc ttcaactaat tcatgaaaaa agggatacgc
    13441 ctc
//
LOCUS       DAFBQW010000070        13429 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFBQW010000070 DAFBQW010000000
VERSION     DAFBQW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.073"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..914)
                     /locus_tag="LK889_002358"
     CDS             complement(<1..914)
                     /locus_tag="LK889_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBK5096561.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="LK889_002359"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="LK889_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK5096562.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="LK889_002360"
     CDS             2006..2176
                     /locus_tag="LK889_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096563.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="LK889_002361"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="LK889_002361"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK5096564.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="LK889_002362"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="LK889_002362"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK5096565.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="LK889_002363"
     CDS             4440..5321
                     /locus_tag="LK889_002363"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK5096566.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="LK889_002364"
     CDS             5318..6034
                     /locus_tag="LK889_002364"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK5096567.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="LK889_002365"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="LK889_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBK5096568.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="LK889_002366"
     CDS             6751..7752
                     /locus_tag="LK889_002366"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK5096569.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="LK889_002367"
     CDS             7749..9110
                     /locus_tag="LK889_002367"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBK5096570.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQKITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="LK889_002368"
     CDS             9070..10221
                     /locus_tag="LK889_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBK5096571.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="LK889_002369"
     CDS             10196..11365
                     /locus_tag="LK889_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5096572.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="LK889_002370"
     CDS             11540..12919
                     /locus_tag="LK889_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5096573.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="LK889_002371"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="LK889_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4486 a         2339 c         2922 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgagaaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacagaaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAFBQW010000071        13395 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFBQW010000071 DAFBQW010000000
VERSION     DAFBQW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.074"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(14..706)
                     /locus_tag="LK889_002372"
     CDS             complement(14..706)
                     /locus_tag="LK889_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5096574.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LK889_002373"
     CDS             complement(1143..1712)
                     /locus_tag="LK889_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5096575.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LK889_002374"
     CDS             complement(1786..2109)
                     /locus_tag="LK889_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096576.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LK889_002375"
     CDS             complement(2263..3075)
                     /locus_tag="LK889_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096577.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LK889_002376"
     CDS             3429..4277
                     /locus_tag="LK889_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK5096578.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LK889_002377"
     CDS             4422..5363
                     /locus_tag="LK889_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBK5096579.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREIPFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LK889_002378"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LK889_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="LK889_002379"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="LK889_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBK5096580.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="LK889_002380"
     CDS             complement(9827..12013)
                     /locus_tag="LK889_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBK5096581.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="LK889_002381"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="LK889_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBK5096582.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="LK889_002382"
     CDS             complement(12235..12675)
                     /locus_tag="LK889_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBK5096583.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="LK889_002383"
     CDS             complement(12749..13288)
                     /locus_tag="LK889_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5096584.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3761 a         2810 c         2382 g         4442 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatccc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaa
//
LOCUS       DAFBQW010000072        13335 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFBQW010000072 DAFBQW010000000
VERSION     DAFBQW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.075"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="LK889_002384"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="LK889_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK5096585.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="LK889_002385"
     CDS             complement(738..1460)
                     /locus_tag="LK889_002385"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK5096586.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERLLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="LK889_002386"
     CDS             complement(1596..2477)
                     /locus_tag="LK889_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK5096587.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LK889_002387"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LK889_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK5096588.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="LK889_002388"
     CDS             3717..4196
                     /locus_tag="LK889_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBK5096589.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="LK889_002389"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="LK889_002389"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBK5096590.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="LK889_002390"
     CDS             5497..5625
                     /locus_tag="LK889_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK5096591.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="LK889_002391"
     CDS             5629..6174
                     /locus_tag="LK889_002391"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK5096592.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="LK889_002392"
     CDS             complement(6628..8319)
                     /locus_tag="LK889_002392"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK5096593.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LK889_002393"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LK889_002393"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK5096594.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LK889_002394"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LK889_002394"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK5096595.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LK889_002395"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LK889_002395"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBK5096596.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="LK889_002396"
     CDS             12601..13302
                     /locus_tag="LK889_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5096597.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3753 a         2710 c         2368 g         4504 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaaagacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taaat
//
LOCUS       DAFBQW010000073        12989 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFBQW010000073 DAFBQW010000000
VERSION     DAFBQW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.076"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(88..1668)
                     /locus_tag="LK889_002397"
     CDS             complement(88..1668)
                     /locus_tag="LK889_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK5096598.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLYQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2069..3445)
                     /locus_tag="LK889_002398"
     CDS             complement(2069..3445)
                     /locus_tag="LK889_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK5096599.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3573..4691)
                     /locus_tag="LK889_002399"
     CDS             complement(3573..4691)
                     /locus_tag="LK889_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5096600.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4833..5267
                     /locus_tag="LK889_002400"
     CDS             4833..5267
                     /locus_tag="LK889_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5096601.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5345..5878)
                     /locus_tag="LK889_002401"
     CDS             complement(5345..5878)
                     /locus_tag="LK889_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBK5096602.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5972..7150)
                     /gene="mutY"
                     /locus_tag="LK889_002402"
     CDS             complement(5972..7150)
                     /gene="mutY"
                     /locus_tag="LK889_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK5096603.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7143..7940)
                     /gene="recX"
                     /locus_tag="LK889_002403"
     CDS             complement(7143..7940)
                     /gene="recX"
                     /locus_tag="LK889_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK5096604.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8047..9429
                     /gene="rlmD"
                     /locus_tag="LK889_002404"
     CDS             8047..9429
                     /gene="rlmD"
                     /locus_tag="LK889_002404"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK5096605.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9603..10181)
                     /locus_tag="LK889_002405"
     CDS             complement(9603..10181)
                     /locus_tag="LK889_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBK5096606.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10503..10973
                     /locus_tag="LK889_002406"
     CDS             10503..10973
                     /locus_tag="LK889_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBK5096607.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11081..12307)
                     /locus_tag="LK889_002407"
     CDS             complement(11081..12307)
                     /locus_tag="LK889_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5096608.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3840 a         2621 c         2186 g         4342 t
ORIGIN      
        1 cgtcacggct ctaaacaaaa tatgagaggc tgtttttgtt tggctattct tattacttga
       61 tgcagggcag cttgtgtcaa gacttcttta catcaaactt tttaatttta cattgggata
      121 tttatccgca aaccagcgtt ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa
      181 acggtcacga gccaagatat tgcgacttga actcatcttc tcatccaaat cttctggatc
      241 gatccagcga gcgattttgt gtcccattgg agacatgatt acttcggcgt tatactcatt
      301 taacatacga tattggaata cttcgaattg tagctgacca acagctccaa tgatatattc
      361 ttccgttaga tatgttttat aaagctgaat cgctccttct tgaactaatt gatagattcc
      421 tttgtggaag gatttttgtt tcatgacatt ttttgcagtc actttcataa acagctctgg
      481 agtaaatgaa gggagttctt cataagcaac ttttagtttt ccttcgaaca atgtatcccc
      541 aatctgataa ttgcctgtat catatacacc gatgatatcg cctgctactg ctttttcgac
      601 gttttctctc gaatctgcca taaattgtgt gacattgctt aatttcagtt ttttatttgt
      661 acgttccaac cagacatcca ttcctcgttc gaatgttcca gaacagattc ggacaaaagc
      721 gattcggtca cgatgggctg gattcatatt tgcttggatc ttgaatacga aacctgaaaa
      781 ttcttcctca taaggactta ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg
      841 tgccaattca acgaaagtct ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc
      901 aaaaaatact ggtgtctgat cgccacgagc aattttttct tgattaaatg catctccagc
      961 ctctttgatt agttctactt cttctaagac ttgttcatat aaggaatttt cagttagagg
     1021 atgattttcg ggaagcagtc catcttcatc caattctact aatcgttcat cctgataatt
     1081 ttccggacga taaaactcta cacgattatg gtagatatca taaagacctt ctagcccttt
     1141 acccataccg attggccaat tcatcggata agattcgata tctaatagct cttctaattc
     1201 ttctaacaat tccaatggtt cacgaccatc acgatctagt ttgttgataa aagtaaatat
     1261 cgggatgcca cgttttttta caacttggta taatttcttt gtctgtgctt cgatcccttt
     1321 tgcactatcg attaccatga ccgcactatc cacggccatc aaagtacgat aagtatcttc
     1381 agaaaaatct tcatgtcctg gtgtatccaa gatatttact cgtttccctt gataatcaaa
     1441 ctgcataaca gaactagtaa cagaaatccc acgttgtttc tcgatttcca tccagtctga
     1501 tttggcaaaa ttacctgttt ttttgccttt tacggtcccg gcttgacgga tagccccacc
     1561 aaacaatagt aattgttctg taatcgttgt tttcccagca tccggatggg aaatgatcgc
     1621 aaaagttctg cgattgtcca cttgttgctt taattttgga ttattcataa cctaactcca
     1681 tttcttccat tcaattattt atattttctc taggcacaga ttgtccgatc tcttaaagaa
     1741 agacaatttc atatagggct gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac
     1801 ggcgttctag agacagccta tcagcaataa gcccacccaa gccaaaaata cgaaaagctg
     1861 tttttgcttg gctgctctta ttacttaatg caggatagct ttttcacaac ctttgtcaac
     1921 tactacaact gaatcagtat agcatggatt cagcgaatga aaaagccacc aaaccataag
     1981 agtcaaatat ttttttgtca gctgactaaa agaaaagacg tgttgcttgt ttattcaagc
     2041 aacacgtctt tcataaccaa gtttccaatt atcttcttgg ctcgtcatct tcgaattctt
     2101 ttctgaagta tcttcgttct tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa
     2161 tgacacggat tttaaggatt cgagaacctt ccatttcttc agaaaccagc gtaatattat
     2221 cgacatcaaa cgataacttt tcgccttcat ccggaatcat gcctaatgct gtaatcaaat
     2281 aaccagccat cgtatctaca tcactcatat gcagatcact gtcaaaagct tcattgaact
     2341 catcaatcag catacgtcct tggatgatat attcgtgttc atcgattttt tcataaagat
     2401 tctcaacttc atctgattca tcatcgattt ctccgacgat ttcttctaaa aggtcttcta
     2461 gtgttgctaa cccaacgacg ccgccatatt catctaataa aatagccatc tgattctgcg
     2521 tctttttcat ttcatataac agatcatcaa taaagaccgt ttcaggaacg aataaaggct
     2581 cctgcatgat tttcttgata tctaaattat caaagccgaa tttatgggca gcttttagta
     2641 aattctttgt atgaaggatt ccgactactt tatctttgtc ttcgttataa acagggattc
     2701 ttgaatagtt ttcggataaa acttcattca cattttcttc cacagtgtcg ttgatatcaa
     2761 tcataaatgc atccgttcgt gggaccatga cttctcgggc aactttcgta tcaagagaaa
     2821 aaaccccttg gagcatttct aattcttcat tattcaatac gccttcgttt tcaagcatat
     2881 aacgcatttc atctctggtc atttttgaat cttcatcatc aaaagtcatc ggcgtgatac
     2941 gcgccaataa acttgtggaa gcagaaagca gccagacgaa gggcttagct actacaccaa
     3001 tcaagcgtat cagccctgaa gtgaactttg cgacttcttc tgatttattt aaagcaatac
     3061 gtttagggta caattctcca aaaacaatcg atacataggt caaaatagcc agcacaatga
     3121 tatttgcaat attttttgcc gctgtgccgc cacctaatac cggagctaac cgttccgata
     3181 aagtattagc tagtgaagca ccagacaaaa tgttgaccaa agtgattcct acttggattg
     3241 tcgataggaa attatttgga ttctgcaata tttttaaaag cttttgagat ttcacatctc
     3301 cttcatcagc tttttgctcg attcgattct tattgatcga aacaacagca atttcggaag
     3361 ctgctaaaaa agcattgatc aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg
     3421 actgactctc agggtcagca ttcataatcg cattctctcc ttataaaatt aatttttagt
     3481 ttacgataga aaaaaacgtg gcactatagc cacgttaaaa tggtatcaca aagcaatttt
     3541 ctttgcaata aaaacaagtg tactaaaact acctacgagg tcgtagcaat tttcttcttg
     3601 ttttcatttt gcttgtcttc tttgacgatt ttgattatat aggaaatgat cgtcttgcag
     3661 attgcgtaaa aaggaacacc taagaagata ccaagcaatc cggcaatatt tccagctact
     3721 agaagaacga taatgatcgt taatggatgg attttcagtg atttcccaat tacattagga
     3781 taaatgatat tcccatccaa ctgttggacg attagcacaa ccacacaaca aagtaatgct
     3841 tttaccggat cattgaagac tgttactaga aatgcaggtg ctagtcctag ataaggtcct
     3901 aaatatggaa ttaggtttgt caatcccgca atgaccccaa ataagaaagc ataatccaca
     3961 ccaattacca tatatcctaa aaatgtgaag gtaccaacga ataaacattc gattgcttgt
     4021 ccgctaatgt aattagccaa tgttttattc agttgcccca ataactccac aatttggttt
     4081 cttctttttt ctgggaaaaa ctgttggatg tttggcacta gtttttctcc gtctttcaac
     4141 atatagaaca agatgaatgg tgctgtaatg attactacgg ttgccgaagc gatcgttcca
     4201 acgatcgaac ctaaactatt ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa
     4261 gaaatgtcta attgctcaaa atatttattg atatctactt ccttgaacaa aggcaattcg
     4321 gctacctctt ttaaccagct ttctacctgt ttgatgaaat caggcatatt agatgccaat
     4381 gaggaaattt gctgtactag acttgggatg acactaagta aaatccaaat aattgctgca
     4441 actaaaagga gcagcacaat tgcgacagcc catattcttt tgatttttgc tttcatcaac
     4501 agcttgacaa taggattcaa taaatagtaa agaaagcctg caatcagtac aggtgcaaat
     4561 aacgtggaaa aaaatgtccc gatcggttcg aaaataaagt ttatttctga tgaaatcatg
     4621 atcaatgcag ctatgatcaa taattcaagc gaccaaaaca ttagtttaga ctgtctaagt
     4681 ttttcaaaca atagatttcc tcctttcttt cttactagta tatattatat tcgttaaaaa
     4741 ttcttttatt ctttttttat ttttgaaaac ttgccatact ttttatttgt tttattttcc
     4801 agtatactat acgaaaggaa ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa
     4861 tgagcaatat ttacttagat gctgtacgga tcaggcatca agtttttgta gtcgaacagg
     4921 gtgtgccttt gtctcgagaa atagacaaag atgaagccca ttgtatccac ttcgtcctgt
     4981 actcagataa aaaagaaccg caaggaactg tacgtctttt gcctttagaa aacggaaaaa
     5041 tgaaattaca gcggatggcg attctttctg aatacagaca ccaagggtta ggaaaaatat
     5101 tgattgaaga agcagaaaac tttgctaaga atcaaggcta taatacgatt ttattagggg
     5161 cacagtccac agctgaaact ttttacgaaa aactaggata tacagcttac ggcgatcctt
     5221 ttgaagatgc tggcatgcct catatttata tgaaaaagac tttataaaaa tttatttcga
     5281 aaaaagcaaa aaacagcaag cttttgagaa aaatctctca ctggcttact gttctttttt
     5341 gtgtttactt acgcgacaat tgcttgtaac ggttatacca aatatcaata tagccttccg
     5401 agaacgggcc atctccatta ttgatccagt caaccagaat cttcacattt tcttttagga
     5461 tgaagtcaat atcgttcggg taattcatca ttttgctgtg catctcatat tcatcgacat
     5521 ccaatagtcg tttctcgccg tcaggaaata ccttgatatc caagtcatag tcgatatatt
     5581 tcaaggcctc atcgtccagc aaaaaaggtg aagctagatt acaataatag gaaaccccct
     5641 tttctctgat cattgctatt acattgaacc agtatttttt atgaaaataa acaatcgctg
     5701 gttcgcgcgt tacccatcgg cgtccatcag attcagtgac taatgtgtga tcattcacgc
     5761 cgatcataga gtgttcgctt gtttttaata ccatggtatc tcgccacgtt cgatgcaatt
     5821 tgccgtcgtg cttgtaactt tggatcgtca caaactctcc ttcttttgga actcccatag
     5881 caacccttcc ttaagcagca ttttgtgctt tgtgtttggg tgttcattac accgacatca
     5941 taattattat atcaataaaa atgagctttg cctagaaagg attcctattg tttcgatttt
     6001 tctctaactg atctaccagt ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat
     6061 caggcttcaa ccacttcgaa gttttgttct cggtaaattc ttgttctgct ccctcggttg
     6121 cccgtccgta aaaaaggaga acatgccatt tcagatgact aaaaatatga gtaacttctc
     6181 ctaaatgtcg tgtctgccaa acaaagagtt cctgcttttc aaatgggaga ttttgctctt
     6241 cttctgctac taggtcatca aacagatcga tttctggctt tgtttcccat tcttttttca
     6301 atcgctcata ctcctcttgt gtcacttcca tcattggaaa tgtccacata ttcgccagca
     6361 atttttggct atctctttct tcaaaataat acgctccaga atgattttga agagctgcac
     6421 tgatataata aacgtctttt ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga
     6481 ttcctcgttt gttagctaaa caaaaggctt ggatcggaca agtctcacac tttggagaag
     6541 ttggtgtaca gatagctgaa cctagatcca tcattgcttg gttgaattca cctggatgct
     6601 tttcatcaat gattttccgc atcgcttcat caaagatttt cctgctagaa gcttttgcga
     6661 tgtctgcttc aatgcaaaac aatctgctga ctactcgcat cacgttgccg tcaactgcag
     6721 gttctggaag tccaaatgcg atacttgcaa tcgcgcctgt cgtatatggt cctattccct
     6781 tcaatgagct aatttcttca ggcgtttgcg gcatttcccc atcaaattct gacatgatct
     6841 gtttagcagc agcttggatg ttccgggctc ttgagtaata accaaggccc tcccatgctt
     6901 ttaaaagctt ttcttctgga gcatttgcta gctcttcaat tgtaggaaac cattccatga
     6961 aacgataaaa ataatcgatc actgtgtcta ctctggtttg ctgaagcatg atctcagaga
     7021 tccagatacg ataaggatca cgattatagc gccaaggcag gtttcttttt tcttgttcat
     7081 accattgaat gaactgatct tgaaattctt ttgtttcttt atctgtccat ttttcccatt
     7141 ctttattcac tttcctcttc cttttccgta atgaatcgct ggatcatatc caaatcaaat
     7201 cctttttgat ataagctttg ctttactttt agatttcttt ttttaggttc aaaccttaaa
     7261 tttcttcgcc atagtttatc tccttgtttg actaaataat catattctat ttcttgatct
     7321 gcttcttgcg gaagatcagc tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc
     7381 atcaatgttt gttggatttt tctcaataat tctttttggc tttttccgtg attttttcca
     7441 aacgcttttt cggctgtctg atgtgcattt tgaacttggt cttcaaatgt atattggctg
     7501 agcgcttgtt caatgatttc aggtttcaaa cctttttgct gcatctgctg cgctaatttc
     7561 tttggtcctt tgtcacttaa gcgcatattc gtgcgcacat aactttcccc atagaccaaa
     7621 tcatcgacaa ggtccaattc ttttaagcgg gctacgattt tatttcgatc ttcttgaagg
     7681 atttcctttt cttttaagta tgttcggatt tctttttccg atcgcaactg ataacttaag
     7741 taattcattg ctaggtgaaa accgaaatca gctccgctac tcttcttgat ctcttggata
     7801 gtttcttcgt ctaattcccg tccttttaat aatcgatagc gtactaatac atcttcagat
     7861 acttgtaaag cttctccttc agataaatct acgcgataga aagggccttt gcctttactg
     7921 attctcgtaa cggttaacat aaacatcctc ctcgaacata tattctctat cataccacag
     7981 aaaatccccg taatcaggta ttatatgtta gaataaacca cagaaaaaag aaaaagggag
     8041 atacttatgg cagaaacaat tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt
     8101 ttaggcataa atggtgaagg aatcggttat ttcaaacggt tgatcatatt tgttccatat
     8161 gcactaccaa aagaagaagt actagtgaaa atcacgaaag caactcctcg ctatgcagaa
     8221 ggagaattaa taaaagtcaa aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt
     8281 tactatgaat gcggtggctg tcaattacaa cacttagctt atcaagctca attggatttt
     8341 aaaaaggatc tgcttttgca agcattggaa aaattcaaac cagcaggctt tcgatcttat
     8401 caacttttgc cgacgatcgg gatggatgag ccttggcatt atagaaacaa agcacaattt
     8461 cagttacgaa aaaataagca gactcaaaaa attgaagctg gtttatacga agcaaactct
     8521 catcagttag tccctataac agactgcttg gttcaagaac ctcgaacaca aaaagtaatg
     8581 aatactgttg ttcaactatt gaataaattt gatgcaccca tctatgatga acggacaaac
     8641 agtggaatct tccgcacaat catggttaga atcggtgtta aaactgggga gttacaagtt
     8701 gtttttatta ctcgcagtaa aaaattcccg caaaaaaacg caatgatccg tgagatcaat
     8761 gaacgactgc ccgaagttgt ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc
     8821 gtgatggggg atgaaacgat ccacttatgg ggaaaagaag ccattgaaga acaaatcaat
     8881 gaagtgactt tcgatctttc tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa
     8941 ttgctttatg gcgaaggaat caaagcatta gatgtccagc ctaatgaaac gatcgtggat
     9001 gcttattgcg gagttgggac gatcggctta agtgtagcaa aacaagccaa agaagtacgg
     9061 ggaatggata ccgttcctca agcaatcgct gatgcaaaaa aaaatgcgga aagattagga
     9121 tatcacaaca ctcgatatga agtcgggaca gcagagcaag ttctgcctaa atggttgaat
     9181 gaaggttttc agcctgatgg tatcatcgta gatccaccac gtacgggttt ggataacgca
     9241 ttgatccaat cattattgaa ataccctcca aaaaaacttg tttatatctc ctgtaatgtt
     9301 tctacacttg ccagagatct ggttaattta agtaaaaagt tcaagattga atatttacag
     9361 tccgtcgaca tgtttcctca gactgcacgt tgtgaagttg tcgtgaagat gacgagactc
     9421 gctgactaaa tgaaagaggt tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc
     9481 aaacaaaaat agctcttcat gttttatgaa tactgtactt gtgaccgaag agttaacagg
     9541 aggacactat ttatttaaaa aaagggttgt gacatagttt ttgtcacagc cctttttgat
     9601 tattattgga tattcacgtc tatcacttga taaaatgcat ttgtcgtatc gctgatattc
     9661 catactgcta aaatcacatt atatcctttt cgatcctgcg gaagtgtgat cgtttggttg
     9721 actgtttttg gtggaacagt tgctttgtcg tctatttttg tgatcaattc aaaatcacta
     9781 aatttcaaag gttgattagg attccaagtt ggtttggtaa tgtagtaatc ccaagtactt
     9841 gtccgatgtt gtgctgttaa attccaagta atatttaatg ttcctggatt aaccatgttt
     9901 ttatgccatc tgttagatgt ctgttcatct aaaggctcaa agccgctgac tcctgcacta
     9961 gcaatttttc catcaatgaa tgtatttttg gtagcttcga tactttgcgg ttcccattgt
    10021 gctcttccga cattttgatt taagtttcct gctgctgatg ttcctaggta tgcacggctt
    10081 cctggagaag taatataacc atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa
    10141 ctaactaacc caataaacaa taacgtacta atctttttca tgtagaaacc tccttcattt
    10201 gataaccaca ttgtaaacgc tttattgtat taacacaaac attatgcttt tataatttta
    10261 tgttgaagta aaaaaggtga aaaaagtaag atgcagatta taacagaagt agacgagaaa
    10321 agtacaaata gaaaacatga gaatcaaatc atatgatctt tttcaaaaac atcattcata
    10381 taaacaaatg ttcaactttt taataacaac aattatcaat ttttttctta cttcacaaac
    10441 taaatacagg tcttgctgtg agccttcatg gtaaactaat attatagaaa ggagttagta
    10501 aaatgaagat gataaaagat attcgttttt gggtttgtgt tataggaatt gtcatattag
    10561 gctttttatc agggttgctt agcggaaatc ccggaaaata ttactattct ttgcaacttc
    10621 caccatttgc accacctagc tggatatttg ggccgatgtg gaccttactg tatatattga
    10681 tgggtatttc tctttatttg ttacttaatc acaacaacaa aaaacagcga aacaatcttg
    10741 tcggtctttt tgtgattcag ttcattttta attttatatg gtctgctcta ttttttaatt
    10801 taagaaatat tttcattgca gcaatcgata ttacactttt agttattttc ttgtcagtgc
    10861 ttatgtatca gttgtggctg catcatcgcc tggcaatgtg gctcatgatt ccttattatt
    10921 tatgggtact attcgcaact ctattgaatt attcaattta ttttttaaac taagcgctaa
    10981 aaagagacgt cctgtaattt aacacataga aaaagtgcca cctaagttag attttttgtc
    11041 taatttaggt ggcactcttt attaaccaaa ctattttttt ctagtaccca cttgttgttc
    11101 catactcatt attctggtca taaccataac cattttgttc ctgtccagcg taaggatcat
    11161 aactttgagt atcttgactt ggtgtatagt actgacttgt atcttctgtt tgctgataat
    11221 aggtattcgg atcttggtac tgatcatatg tgttttctga ataggaagaa taatcggtgt
    11281 acccttcact attcgatcca tcaccgtttg tcgaatcgcc atcttccgaa tcatcaaaga
    11341 acatcattga agcattctca tttttcaaat taggtaatgt atcgcttgtt tttatattta
    11401 attgtttctt cagttcattt tggatcgaaa gaagatcatt ttttcctaaa atttgatagt
    11461 agatatcatt gatcatttgt ccttcgcctt gcaactggtc ttgtttgatt gtttcaaacg
    11521 ccggggtgta gttcgtcgct acatcaagca tgtcatccca atctaagtcc gtcgtcacat
    11581 tgttctcaac tgcttttaaa atttttctat aattgcttac gccatctaaa cttaaaactt
    11641 tattgacgat tttcgtcact acttcccgct gacgacgttg tcgaccgata tcgccttctg
    11701 gatcttcgtg tcgcattcga gaataggcta aacctttttc tccattcaat gtttgttcac
    11761 ctactggtac atggacacca tccaaagtaa agtcaatttt attattcact ttgatgccac
    11821 cgaccgcatt gatcaaatct tccatgccat ccaaattgat cgttacataa tgatcaatag
    11881 gaatatcgag cagttgctca attgaatcca tggccatttc gacaccacca aatgcgtagg
    11941 catgatttaa tttatctacc gttccataac ctacgatatt cgtataaata tctcggtcca
    12001 aactgatgat cgttgatttt ttttgtttcg gattaactgt tactaccatc atcgtatcag
    12061 aacgtccttg ttccgttctt cctaatccac cggtatctaa acctaatagc aaaaccgaaa
    12121 aaggttcttt gtcatcaatg cttactctct cagaacgttt ggaggatatt cgattagagt
    12181 ttttagaaat tttgtttact gtttggctcg cttctcccat aaatctgatt ccgtatgctg
    12241 ataatccggc aatcagcaaa acgaataccc ctaacaaggt taatagaact ttttgccatc
    12301 gcttcataat acacctctgt ttcacatttt ctaaaatatt gtctcatata actgttcaat
    12361 cagcaaattc actttttaat cataagaaaa ctttaaaaaa catttttgtg taacgattat
    12421 tattttttaa aatttctcaa aattcttaac tatcatacca taccgtgaaa aagatcgaaa
    12481 gaccgaatac acgcagttcg caatcgtttt atcttttttt atgtattttt tccagaaaaa
    12541 gcgaacaaca aggtattttc caaataatcg tgcagtaaaa tgatcgcaca tacctttccc
    12601 atctttttaa cagtctttgc ctaccgttca gattatcatt cgatttcacg acaatttctt
    12661 cctatgactg gtccttttgt taaacccatc ttctattcgt tttatttcca gctcactgca
    12721 tggctcttaa aatgactcga actagtgatt gcattaccat catcttttaa tggatagata
    12781 tgctcctctt caaagtaaaa cgattttctt ttaaagtaat agaattgaca tggtaatgag
    12841 tatgtatact agtggtttca caaatggtct tttattcaac atccaaaaac tgaaatttct
    12901 ttaaaatcca ttactaacga agaaaatgtt ccttgcttga taattagtta ggacatgtca
    12961 agtgaacaaa tgcatttact tagccaaaa
//
LOCUS       DAFBQW010000074        12842 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFBQW010000074 DAFBQW010000000
VERSION     DAFBQW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.077"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1835)
                     /locus_tag="LK889_002408"
     CDS             complement(<1..1835)
                     /locus_tag="LK889_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5096609.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="LK889_002409"
     CDS             complement(1832..2536)
                     /locus_tag="LK889_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5096610.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="LK889_002410"
     CDS             complement(2548..4857)
                     /locus_tag="LK889_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK5096611.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="LK889_002411"
     CDS             complement(5052..5516)
                     /locus_tag="LK889_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096612.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="LK889_002412"
     CDS             complement(5516..6142)
                     /locus_tag="LK889_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5096613.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="LK889_002413"
     CDS             complement(6149..6514)
                     /locus_tag="LK889_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK5096614.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="LK889_002414"
     CDS             complement(6504..6842)
                     /locus_tag="LK889_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096615.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="LK889_002415"
     CDS             complement(6832..7158)
                     /locus_tag="LK889_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK5096616.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="LK889_002416"
     CDS             complement(7136..7417)
                     /locus_tag="LK889_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK5096617.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="LK889_002417"
     CDS             complement(7419..8783)
                     /locus_tag="LK889_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK5096618.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="LK889_002418"
     CDS             complement(8796..9365)
                     /locus_tag="LK889_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBK5096619.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="LK889_002419"
     CDS             complement(9337..10524)
                     /locus_tag="LK889_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5096620.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="LK889_002420"
     CDS             complement(10588..12315)
                     /locus_tag="LK889_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK5096621.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="LK889_002421"
     CDS             complement(12312..12764)
                     /locus_tag="LK889_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBK5096622.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
BASE COUNT         3557 a         2794 c         2010 g         4481 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 ca
//
LOCUS       DAFBQW010000075        12301 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFBQW010000075 DAFBQW010000000
VERSION     DAFBQW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.078"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            31..1062
                     /locus_tag="LK889_002422"
     CDS             31..1062
                     /locus_tag="LK889_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK5096623.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            1059..1826
                     /locus_tag="LK889_002423"
     CDS             1059..1826
                     /locus_tag="LK889_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK5096624.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1926..3494
                     /gene="liaX"
                     /locus_tag="LK889_002424"
     CDS             1926..3494
                     /gene="liaX"
                     /locus_tag="LK889_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBK5096625.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3791..4111
                     /locus_tag="LK889_002425"
     CDS             3791..4111
                     /locus_tag="LK889_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBK5096626.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4111..4461
                     /locus_tag="LK889_002426"
     CDS             4111..4461
                     /locus_tag="LK889_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK5096627.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4628..5566
                     /locus_tag="LK889_002427"
     CDS             4628..5566
                     /locus_tag="LK889_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBK5096628.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5581..6411
                     /locus_tag="LK889_002428"
     CDS             5581..6411
                     /locus_tag="LK889_002428"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK5096629.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6455..7480
                     /locus_tag="LK889_002429"
     CDS             6455..7480
                     /locus_tag="LK889_002429"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK5096630.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7495..8433
                     /gene="galU"
                     /locus_tag="LK889_002430"
     CDS             7495..8433
                     /gene="galU"
                     /locus_tag="LK889_002430"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK5096631.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8558..8998
                     /locus_tag="LK889_002431"
     CDS             8558..8998
                     /locus_tag="LK889_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBK5096632.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            9001..9603
                     /locus_tag="LK889_002432"
     CDS             9001..9603
                     /locus_tag="LK889_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5096633.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9662..10768)
                     /locus_tag="LK889_002433"
     CDS             complement(9662..10768)
                     /locus_tag="LK889_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK5096634.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            10978..11979
                     /gene="ccpA"
                     /locus_tag="LK889_002434"
     CDS             10978..11979
                     /gene="ccpA"
                     /locus_tag="LK889_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBK5096635.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         4088 a         2118 c         2595 g         3500 t
ORIGIN      
        1 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaattagcat catcactata
       61 ttttttattt gtgtgcttac ttcatgggtt attcagcatc aagccgcttt atatgaagta
      121 cctgtcatcg agatagaaaa agtacgaata aaagaggatc atcaattgat tacaggaaaa
      181 ctgatgaaca cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat
      241 caaatagaaa cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat
      301 gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc
      361 attggcgcca tgttgcttat tggtggaaaa cagggtctat ttgctttagg aagtctcatc
      421 atcaatatcg ttttactttt acttttgttg ctcatcttta ctaaaacaga agggatttct
      481 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
      541 gatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
      601 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
      661 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
      721 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
      781 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
      841 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
      901 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
      961 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
     1021 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
     1081 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
     1141 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
     1201 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
     1261 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
     1321 tttgtttcct actattgctt cctatcactg attttttagc aatccaaggt tttacatcgg
     1381 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
     1441 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgcgagt gtagcaattt
     1501 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
     1561 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
     1621 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
     1681 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
     1741 tgtccgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
     1801 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
     1861 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
     1921 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
     1981 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaatcaaaa
     2041 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
     2101 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
     2161 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
     2221 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
     2281 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
     2341 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
     2401 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
     2461 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
     2521 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
     2581 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
     2641 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
     2701 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
     2761 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
     2821 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
     2881 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
     2941 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
     3001 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
     3061 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
     3121 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
     3181 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
     3241 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
     3301 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
     3361 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
     3421 cagatggtga aatcgcgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
     3481 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
     3541 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag tctatctctt
     3601 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
     3661 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
     3721 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
     3781 gaggtgagac atgatgaaaa aaaagttgac aaaatctaat aaaaatgttg ttttgacagg
     3841 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
     3901 tgtattcctg agcttagttg cttttggaag tccaattatt ttatacattt tattagcttt
     3961 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
     4021 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
     4081 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
     4141 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
     4201 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
     4261 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
     4321 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
     4381 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
     4441 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
     4501 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
     4561 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
     4621 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
     4681 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
     4741 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
     4801 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
     4861 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cagctccaga
     4921 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
     4981 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
     5041 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
     5101 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggccatcgat tgatcgcaga
     5161 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
     5221 cctggaacat ctgattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
     5281 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
     5341 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
     5401 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
     5461 agtagccgcg atgaatttcc gtgcacgtac aatgggcttt gacgcaacga aaacatttga
     5521 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
     5581 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
     5641 gcattgatca tcgtatcagg tatcattatc gctatgtggc ttagcagcag agaggctgtt
     5701 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
     5761 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
     5821 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
     5881 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
     5941 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
     6001 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
     6061 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
     6121 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
     6181 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
     6241 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
     6301 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
     6361 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
     6421 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
     6481 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
     6541 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
     6601 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
     6661 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
     6721 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
     6781 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
     6841 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag caaaacatga
     6901 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
     6961 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
     7021 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
     7081 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
     7141 agcaatgggt gccaacccgc tgacctttat cggattaagc ggaataggcg atctagtcgt
     7201 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
     7261 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
     7321 agcagcgatg gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta
     7381 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
     7441 tggaaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
     7501 gttaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
     7561 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
     7621 gaagcgttga aatcaggtat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
     7681 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
     7741 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
     7801 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
     7861 ccatttgtcg ttatgctagg cgatgatata atggaagacg aagtaccatt gacacagcaa
     7921 ttaatcgatg attacgaacg tactcatgct tctacgattg ccgttatgca agttcctcat
     7981 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
     8041 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
     8101 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
     8161 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
     8221 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
     8281 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtacatc
     8341 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
     8401 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
     8461 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
     8521 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
     8581 attgatcgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
     8641 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
     8701 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttac tggtcaaaag
     8761 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
     8821 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
     8881 gaaagtcggc ggaatcggta aaacgacagc taaagcgacg gttgccggaa aagtcggtgg
     8941 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
     9001 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggcacagc cgcagcaatc
     9061 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
     9121 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
     9181 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
     9241 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
     9301 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
     9361 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
     9421 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
     9481 atgtcagaag caaaaccagt tgcagatgac tcaaaagaaa tgatgaaaat ggcaaaagaa
     9541 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
     9601 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
     9661 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
     9721 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
     9781 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
     9841 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
     9901 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
     9961 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtag attttttctt
    10021 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    10081 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgta tttcctggtg
    10141 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    10201 aaacaccttg ttttttcaat tggtattcaa tttctgcaat gatcgcttgt tcagtcacgc
    10261 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    10321 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    10381 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    10441 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    10501 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    10561 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    10621 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    10681 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    10741 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    10801 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    10861 aattttcatg aaaacagaga taaaacggtt gcaattcttg agattatttg atattctatt
    10921 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
    10981 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
    11041 gtttcacgtg tcgtaaatgg caatccaaac gttaaaccag caacaagaaa aaaagtcttg
    11101 gaagttatcg atcgcttgga ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag
    11161 aaaacaacaa cagttggtgt aatcatccct gatgtcagca atatgttctt tgcttcttta
    11221 gcacgtggta tcgatgatgt ggcaacaatg tacaaataca atattatctt agcaaattca
    11281 gatggcaacg accaaaaaga agtcaatgta ttgaataact tactggctaa gcaagtggac
    11341 ggcgtgatct tcatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
    11401 aaaactcctg ttgtattagc tggctctatt gatccagatg aacaagtagg tagcgtaaac
    11461 attgattatg tatccgcaac aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa
    11521 aaaatcgctt ttgtcagtgg tgcgttgatc gatcccatca atggacaaaa tcgtctgaaa
    11581 ggctataaag aagcattaag cgaaaataac ctaccatata gcgaaggatt gatctttgaa
    11641 gcacaataca acttcaaaga tggtctatct ctagtcgatc ggatccataa tagcggtgca
    11701 acagctgtct atgtttcaga tgatgaatta gccatcggta ttttggatgg tttattagac
    11761 cgtggcgtga aagtaccgga agaattcgaa atcatcacaa gtaacaattc actactgaca
    11821 gaagtagctc gtcctcgttt aacaagtatc acacaaccat tatacgattt aggggcggta
    11881 tctatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
    11941 ttgccttatg gaatcgaaga aaaaggatct acaaaataat aaaaggacaa ccattcagag
    12001 gttgtgtgcc tgccgtttaa ctctgcgtta caaggagcaa tttcaaaaaa cagcatctca
    12061 tgtttgatga atattgtgac tcgtgattcg gagttggcag gagaacaccg tttatttggg
    12121 aaaaggggtt atgacaaaat tttgttataa tccttttttt gtgtcttaaa aaagagagaa
    12181 agtggttgaa attacattta tcgtatataa aacaatccgt gtatagatgc tgcattttgt
    12241 tcaccaccat caaaaaaata agggactgtg acaatactat tgtcacaatc ccttattact
    12301 g
//
LOCUS       DAFBQW010000076        12141 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFBQW010000076 DAFBQW010000000
VERSION     DAFBQW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.079"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            124..1935
                     /locus_tag="LK889_002435"
     CDS             124..1935
                     /locus_tag="LK889_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096636.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            1996..3465
                     /locus_tag="LK889_002436"
     CDS             1996..3465
                     /locus_tag="LK889_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK5096637.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3476..5485
                     /gene="topB"
                     /locus_tag="LK889_002437"
     CDS             3476..5485
                     /gene="topB"
                     /locus_tag="LK889_002437"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5096638.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            5601..5750
                     /locus_tag="LK889_002438"
     CDS             5601..5750
                     /locus_tag="LK889_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096639.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            5831..6427
                     /locus_tag="LK889_002439"
     CDS             5831..6427
                     /locus_tag="LK889_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK5096640.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6440..7339
                     /locus_tag="LK889_002440"
     CDS             6440..7339
                     /locus_tag="LK889_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK5096641.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7342..7473
                     /gene="ssb"
                     /locus_tag="LK889_002441"
     CDS             7342..7473
                     /gene="ssb"
                     /locus_tag="LK889_002441"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5096642.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7495..7827
                     /gene="ssb"
                     /locus_tag="LK889_002442"
     CDS             7495..7827
                     /gene="ssb"
                     /locus_tag="LK889_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5096643.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            7872..8105
                     /locus_tag="LK889_002443"
     CDS             7872..8105
                     /locus_tag="LK889_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096644.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8264..8386)
                     /locus_tag="LK889_002444"
     CDS             complement(8264..8386)
                     /locus_tag="LK889_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBK5096645.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            8576..8800
                     /locus_tag="LK889_002445"
     CDS             8576..8800
                     /locus_tag="LK889_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096646.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            9243..9449
                     /locus_tag="LK889_002446"
     CDS             9243..9449
                     /locus_tag="LK889_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK5096647.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9449..9700
                     /locus_tag="LK889_002447"
     CDS             9449..9700
                     /locus_tag="LK889_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096648.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            9716..9871
                     /locus_tag="LK889_002448"
     CDS             9716..9871
                     /locus_tag="LK889_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096649.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            9881..10153
                     /locus_tag="LK889_002449"
     CDS             9881..10153
                     /locus_tag="LK889_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096650.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10598..10777
                     /locus_tag="LK889_002450"
     CDS             10598..10777
                     /locus_tag="LK889_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096651.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            10836..11192
                     /locus_tag="LK889_002451"
     CDS             10836..11192
                     /locus_tag="LK889_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096652.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         4441 a         1999 c         2520 g         3181 t
ORIGIN      
        1 gaaaaaggaa aagaaaacct tcttcctgaa gtacaagcaa tcattgacag cggacgagtg
       61 acgcaatgga aaaaaaatcc gaccatttat tttctgaaag gctatcgaaa agtcgcttta
      121 gaactgaatg aaaacgggga atttgaggaa tctaaaaaat accaagccac gtcggaagag
      181 gaaaaagcag ctgttcgtga attacttgaa gaggctgtac caactgattt tttgaaggag
      241 aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa atgatccaga aaaaggattg
      301 aaaagttatg gtggggagat cgtaacattc gaattgatcg atcaactcaa aacgttggat
      361 aaccaatacg ccgcagaagc aggttattat aaattctatt ttgaagaagt gaagaacgga
      421 gaagtagtcg atcagatccg aatggatcta ggggatggcg tcaacagtaa tcaacccatc
      481 tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga aggaacaaaa acaatcggtc
      541 aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag aaacaaaaac aaaacaaaag
      601 ggctatcaaa aacgaacggc agaagagatt aaacaagaag ttaaatccct ttcgcaacag
      661 gcactggatg cggtgaaaaa gcatactcat tctcctgaag acgtcaaaga acttttgaac
      721 tttatgtctc gttttccaga acgatccttt agaaatcaat tattgattga ggaacaattt
      781 ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa aagaaggaat ctatatcaaa
      841 aaaggggaac aagggaacaa gatctttgtc agaaaaacgg tcaaaggatt ttatgagaaa
      901 ggacgtggct ttgttcgaga aatagaagct acaccagaag ataaaaaacg aatcgaaagt
      961 ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg aaaaagtgtt tgatatcaca
     1021 caaacacagt taaaacctga agactatccg aaaatctttc cgaaccgtgt ctttgatttt
     1081 caattggata aaaaaggaca gtcagaactt caagctggga ttcaagcggt agccaacaat
     1141 attggaatac aaattcgaga tatgacagaa agtgaagtct atcaaagaga attaggacaa
     1201 gcacgtgggg cgtatgttcg taacgaattt actggcgaag aagaaatcgt catgaatact
     1261 cgaaatactc ctacgcagca tttagctaca agtattcatg aattagccca tgcaagaatg
     1321 cacaaatttt cagaattaga taccgcaact aaagaattgc aggcagaaat gacttcttat
     1381 attgtgtgca aacattttgg aatggacacg ttggagaaag ctattcctta tattgcagca
     1441 tggacgaaga atggtcagac gttggatgat aaggaagctg cggaacgtgg gaaaatcatg
     1501 aatgatgtca gccgtgtcgc aaatgaattc attcaaacca tttctaatga aatcaatcaa
     1561 tacagagaaa gagaaccaga gatccaacca gcagaagtga agcaggagca taaaaatact
     1621 gtagaaacgg cagaacaaaa gaatattgtg cctgttggtt cactttggat cgacaagaaa
     1681 gacgggaaag taatggaaga agatacgggt cgaacgttac atttgaagga tgcggcgttt
     1741 attgaagatc cacagcataa tcaagtaatt ttaaaaggaa aaagttatgg aaaagagatc
     1801 caaacggcgt taccttcaga agaatttgcg ctaggaaatt cattccgatt ttcacttgag
     1861 acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt atgaacaaca attgtcacaa
     1921 aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa aacggatagt cttttttctt
     1981 ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta ttgatcgatg taaacagatc
     2041 gatatagtgg attttgctcg aaacaatgga atggctgtag tcaataaagg acgagactat
     2101 cgcttagaag accatgattc atttgttttt gatcgaagaa aacaacgttt ttattggaac
     2161 agtcaaaata ttagtggcga cattattgaa ttagctaaac tttttttcat agacaaagag
     2221 atccaagacc ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac
     2281 aaaacagaac gagtggaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt
     2341 gattatcaac cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca
     2401 gagtggctga ttgattatgc agaaaaagaa gggttgattg ctgaactaaa gccgaaacat
     2461 gaacggcaaa attttttagt tggagacgac cgtttagatc atgcggttgc ttttttatgg
     2521 aaagatccac agacaggaga gacagtaggg gcaagttatc aaggaacgat cgtcgatttt
     2581 aatcgtttcg gtaaacgggg aacgtataaa cacattgaca agaatcctac gccaaatcac
     2641 ggatttaatt taaaaatagg cgatccgaag catttaaaat tctttgaaag tagtattgat
     2701 ctgttaagtt acgctgcatt gaatcgggag aaactacaag atgcctggtt agtttcgatg
     2761 gacgggttaa aacatcatgt aatcagtcat tacgttgaag agtcgatttc tgaattaaga
     2821 cgaaaacaaa catttcctca atcgattgaa atatgtgtgg acaatgatcg tgcaggacat
     2881 attttttatg aaaaagaaca gatgaaggga atcgttgatc cttttaccaa taagaaaatc
     2941 cgttgtgagc gaggaattcc aaatgattgg caagtaccta aagaatacaa agctacttat
     3001 gaagcagtcg caaaagaaat gagtgttgaa cctgaagcaa ttatggccat acacaaaact
     3061 gaaactaatt tacagctgac aaatcaattg gtgtctgctc atgatgttca atctacgttt
     3121 ggaaagatgt tggcgaaggg cgaaccagta gaaacaattg atttgaaaga agcatgtacc
     3181 acggtcgcga aggaactgaa agtatgtgaa cgcgcagatg gaacatacaa ctttgatcgt
     3241 ttttatagta gaaaagccaa cataaaagac gttaatgcag gaatccttct ttcctataaa
     3301 gcagaacaat actacaaagg ctataaaaag catgaacatg aatttgtacc tgaagtgaaa
     3361 aaggattgga atgatcaatt gaagcatgag atccagcaac aagaaatcag aaagcaaaaa
     3421 cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg agtgaagtcg tcactatgaa
     3481 aaccgtcatt ttagcagaaa aaccgtcaca agcgaaggct tatgccgata gcttttctaa
     3541 agctacccga aaagatgggt attttgagat ccaagatcga ttgtttacag gagaaactgt
     3601 gatcacgtat ggttttggtc atttagtcga gttagattcg cccgatatgt acgatgaaaa
     3661 atggaaacaa tgggcattag aacatttgcc tattttcccc aatcaatacc actatcatgt
     3721 tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga cagctgcagt cagctgatac
     3781 gattgtgatc gcgactgata gcgatcgtga aggggagctg attgcctggt caatcattca
     3841 acaagcaggc gccaatcaag gaaaaacctt taaaagactt tggatcaact ccttagaaaa
     3901 agaagccatt tatcagggat tccaacagtt acgagacgca gaagagacct atccaaaatt
     3961 tgaagaagta caagcacgtc agatcgccga ttggttaatt gggatgaacg gtagcccact
     4021 gtatagttta ctcttgcagc aaaaaggcat accaggaagt ttttctttag gtagagtcca
     4081 aaccccaaca ctctatatga tttatcaatt gcaggaaaag attagaaact tccaaaaaga
     4141 accatatttt gaaggaaaag cccaagtaat cgctcaaaat ggcgcatttg atgcgaagct
     4201 tgatccaaat gaaacccaag cgacacaaga agcatttgaa gcctatttaa aagaaaaagg
     4261 tgttcaatta ggaaaacagc caggtacgat ccttcaagtc gaaacagaga aaaaaagtgc
     4321 ggctagccca cgtctttttt ctttatcgag ccttcagtct aaaatgaatc aactcatgaa
     4381 agctagtgcc aaagatactt tggaagccat gcaagggttg tatgaaggaa aatatctaag
     4441 ctatccgcga acggataccc cttatattac ggaaggggaa tatgcgtatc tgttggatca
     4501 cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt ccgactccta ttcatacacc
     4561 gaatagtcgg tatgtgaata ataagaaagt ccaggaacat tacgcgatca ttcctacaaa
     4621 aacggttatg acagcagcgg cttttgaaca actaagtcca ctgcagcagg ctatttatga
     4681 acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag tatacctatg aagaaacaac
     4741 gatcttgacg caagttcagc agcttcagtt aaaggcaatc gaaaaggtgc cgctagatct
     4801 aggttggaag aagttatttg gaaaagagag cgacggaaaa gagaaggaag aagagccgct
     4861 acttcctaaa gtgacaaaag gagaaatggt gacggtggat ctacaagttc ttgaaaaaga
     4921 aaccaagcca ccacaacctt ataccgaagg tacgttgatc actgcaatga aaacagctgg
     4981 gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa gaagtggaag gaatcggtac
     5041 agaagcgaca cgggcgaata ttattgaaac cttgaaacaa aaggaatata tcaaggtaga
     5101 gaaaaataag ctcgtggtga cgaataaagg gattttgctt tgccaagccg tggaaaaaga
     5161 gccgctgctt acgagcgccg aaatgaccgc caaatgggaa agctacttgt taaagatcgg
     5221 tgaacgaaaa ggaacacaaa ccacttttct agccaatatc caaaaatttg tctctcactt
     5281 attggaagtc gtaccagggc aaatccaatc cactgatttt ggatcgacgt tacaagaagt
     5341 gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta ggaatttgtc caaaatgcca
     5401 ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt acgtctgaag cctgtgattt
     5461 taaaaggtca gaagggttca tttgaagcaa cgattgtttt aaaagaagac ttcactacag
     5521 gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa aagaacacgc cgcactgcaa
     5581 aataatggag ggttaatctt atgcgtaata ccacacacaa acaacaagaa atcgaaaatc
     5641 aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc cagcaaacga agactgcagc
     5701 aattgaagaa ccagcagagg attcaaaaag aaatcgaatt agatcgataa aatcttccta
     5761 actattgtat acgtataaac ttatgttata ctgtttttat aagtttatac gtatacaaag
     5821 gagggagaaa atgacaaaag caatggtata caagctgcta gaagaattta atggaacttt
     5881 ggctttgaag gatgccaaaa aagcgggcat ttctcctgta acgatcaaac gtatggtaga
     5941 tcgaggggag ctggatcgag agtatccagg ttttttcacc ttacctggtc aatttcctga
     6001 tgaattgttt atggcgcaaa aaaaatatga acgcggcatt atttcacata ttaccgcgtt
     6061 agatttgtat gatttaacag atatgatccc tagacagatc gatctgactg taccttacgg
     6121 ttatcacgtt tctgaaaaag gattgaagga attcgcagtt gagctacact atacaaaagc
     6181 ggaatggtat gaattaggta agattgaaat aaaaagtcgc tatgggaatc ctattatagc
     6241 ttatgatcca gaaagaactt tatgtgacat ttggaatccc tggtataccg ttgaggatga
     6301 aatcaaagta aaagcaatca aaaactatat ggagtcggac agaaaaaatc ttagaaaatt
     6361 gaatgagtat cgacgaattt taccaacaga caaaacgatg cgttcttata ttatggcttt
     6421 aaattaaagg agaagataca tgacacctac gcagttaaaa gcaaaagtga agaatatttc
     6481 tagagaaaaa gaagttgatc cgcagcttgt tttaagacat tttatgatgg aaaaattctt
     6541 ggaaaaaatc tcagagtctc cttatcgtga aaattttgtg ttaaaaggcg gcttcttgat
     6601 tggctcaaag tatggcattg aaaatcgcac aaccaaagat attgatacga ctttacggga
     6661 aatgaaagta accaaagaaa cattaacaac ggttctaaac gaaatttttt caacgccgac
     6721 gaaagaagac attcaatttg agattcaagg aatgaaagaa acgagagaag ccgattacta
     6781 tccaggattt agcttacgtg tgctggcaca tttggaaaac atgcggccag attttaaggt
     6841 agatgtgact acaggtgact cgatctatcc tgcaacgatc actcacagtc ataaattgat
     6901 gtttgaggat cgaacgattc ctttggaatc ctatcctaca gaacaaatta ttgctgaaaa
     6961 attaagtgca acctttgatt ttttaacaga taattcgaga ggaaaagatt tttatgatct
     7021 ctatactatt cctaaaatgg agaagatcga tgagaagacc ttgaaagata gtgtgagaaa
     7081 tacatttaca cgtcgaggaa aaactgatcc gttaaagata tactatgaaa aggatatgga
     7141 tgtactgaaa aatagtcacg agctaaaaga tacctggaag aaatatcaaa aaagcaatcc
     7201 ttatgctgcg tcgatcacgt acgatgagac aatggattcg attagtaatt tgatggaaaa
     7261 agtgattcac gtcgaagaaa aagagcgaaa actagcttat caaagacacc aacagatgaa
     7321 tagagaaatg gagagataaa gatgattaat aatgtgacat tagtaggacg cttaaccaaa
     7381 gatcctgatt tacgttatac cgcaagtggt actgcagtag cgacttttac cctagccgtc
     7441 aaccgaaatt tcacgaatca aaacggcaat tgagaagccg attttattaa ctgcgtgatt
     7501 tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa aaggtagctt gattggtgta
     7561 gttgggcgga ttcaaacgcg gacttacgac aaccagcaag gacaacgtgt atatgtgact
     7621 gaagttgtag cagataactt tcaactatta gaaagtaaag cagcgacaga gagccgtgcg
     7681 cacgctgatc aatcgtccac cagtccaagc accgccacct ttgaacaacg gaatacagcg
     7741 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
     7801 attagcgatg atgatctacc gttttagttt gagaaagaag gagtaagcat ggcaaaaaaa
     7861 ttagtctgac gatggatcta ttgatacccg taattgcatt tatagacggg atttctttgg
     7921 gcgtcactga aaatcctttg gtaaatagaa ttgcgaaagg atcactattt cttgtattcg
     7981 tgtatcttgt ctgttcttat cttttaaaaa gagcaggaaa ggataattcc ttttggcgat
     8041 tgactaaagt cacagtagca gcgacagctg ttatacttac ggctattcta ccgctttata
     8101 tgtagagtta acgggaaatc gttgaacttc ttgcgaaata ttgctataat gaacatgaaa
     8161 aagaggagtt gcgctaacaa cccctcatta acagcaccga ttaaagaacg gtggcagaat
     8221 attagttatt tggaaataac cgctgacctg ctaaagtaga cggttatttc tttttgtcat
     8281 ttttaagcat atttacaatc aaactaatca aagcaatggt aaacatacca aaaccaagaa
     8341 tggtttgaat tgtctcatat gcggacaaaa ggcttactcc tttcatagat tttagcactt
     8401 acattcataa gcaccaccac ctttccagaa gtcgccaccg tcttcaaact ttactgtctt
     8461 gactattata gcatatctaa aatcgaataa aaattctcaa ttttagctgt ttttgctata
     8521 attttagcga ggtgatcgtg atggaacaat atattggttt actcgataaa ttagggtgat
     8581 caagacacag ccattgcttg tgcgatttac attacgcacg agtcatgaac caatcaattg
     8641 tgtagtggcc aatatagaaa tagcggataa attgctgatt ttggaagaag gcaagtatac
     8701 tatttcagtg acaggtcact tcaataaacg aaagcaacta gtcattgaat caatgcaaat
     8761 tagaaaccct gatcatttta cgcgatcgat gagcatataa aagaagagct gcagtcctaa
     8821 atgaggattg tagctctttt tgttttacat agaaacagta aaaatgaaaa ggcctgttta
     8881 ggaaggcaca agcgccttct caattgaaag acctttctct cactccaacc gccaccgccg
     8941 ttcgagaaga aaggaagtga acgctacgcg ttcactaaga taaagggtct tgcatagcaa
     9001 gacaaaatag aaaaaatttc ctctcaccaa aaggttcgct cctgttcgtt atctctttat
     9061 tgatttgtca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt
     9121 aaatggcacg ctgcgcgccc ttgtcttgtc gcttttcgtt gattcattct ggccaacaaa
     9181 tcaataaaga aagaactaac gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa
     9241 tcatgcgaaa gaaatttgat tattggggtg ttcccttcag cgaattattc ccaaactatc
     9301 atgcaccaca tactgtcgaa tgcgattgtg gagaaagagc gaaatgtatc aaaagctatt
     9361 gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc gattatattt
     9421 taattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat tgactgtaaa
     9481 agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag cagaagtttt
     9541 acttattccg cggtactgga cgatagaaac ccctttagta gaggatgcac cgactatgct
     9601 atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa acttggaaac
     9661 ttatgaaaac gaatattggt taaccaaatt agagaactaa aaaggagaaa aaaatatggc
     9721 tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga
     9781 ggatatgacg ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt
     9841 gtcggtagct gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta
     9901 ctatcgaaca ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact
     9961 atgcttggaa gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga
    10021 actaacgtat gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt
    10081 agattatttt gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa
    10141 cgaaaatgaa taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga
    10201 aaaaaaggtc aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa
    10261 agcctagtaa ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg
    10321 cttaggtggt gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca
    10381 tttgaacaag attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga
    10441 tagtgcagct gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat
    10501 cttttggcgg tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt
    10561 tttcataggg ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa
    10621 acggttaccc gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa
    10681 gtatggcaaa agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga
    10741 aaaagcagga tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat
    10801 ataagtgata tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt
    10861 acaaattaaa gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc
    10921 atttgtatag agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag
    10981 ctggggatcg atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc
    11041 tttggcttat tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact
    11101 tagataagct aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt
    11161 tagaaagtga cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat
    11221 tattttatta agaatgatgg aatataagga taaggttacc aacataacga tcattacttc
    11281 tctgatatgc tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat
    11341 actgcaagcc ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat
    11401 atataagcta tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag
    11461 ttatcaaaaa gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag
    11521 taggatttga catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg
    11581 gagataagat gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac
    11641 cgcaactgaa gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg
    11701 ttaccttaaa atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga
    11761 tggggtacgt gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat
    11821 aaatttagac atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat
    11881 tatagtaatg attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa
    11941 aattgagaaa tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat
    12001 gaagcttacc aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc
    12061 tttgaggaga tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg
    12121 cggtggaggt aaaggttttt a
//
LOCUS       DAFBQW010000077        11882 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFBQW010000077 DAFBQW010000000
VERSION     DAFBQW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.080"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            184..1182
                     /locus_tag="LK889_002452"
     CDS             184..1182
                     /locus_tag="LK889_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5096653.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1294..1803
                     /locus_tag="LK889_002453"
     CDS             1294..1803
                     /locus_tag="LK889_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK5096654.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1833..2492)
                     /locus_tag="LK889_002454"
     CDS             complement(1833..2492)
                     /locus_tag="LK889_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBK5096655.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2494..3258)
                     /locus_tag="LK889_002455"
     CDS             complement(2494..3258)
                     /locus_tag="LK889_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBK5096656.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3266..3943)
                     /locus_tag="LK889_002456"
     CDS             complement(3266..3943)
                     /locus_tag="LK889_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBK5096657.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3980..5365)
                     /locus_tag="LK889_002457"
     CDS             complement(3980..5365)
                     /locus_tag="LK889_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5096658.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="LK889_002458"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LK889_002458"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="LK889_002459"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="LK889_002459"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK5096659.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="LK889_002460"
     CDS             6304..7161
                     /locus_tag="LK889_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096660.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="LK889_002461"
     CDS             complement(7242..7577)
                     /locus_tag="LK889_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096661.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="LK889_002462"
     CDS             complement(7644..9014)
                     /locus_tag="LK889_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBK5096662.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="LK889_002463"
     CDS             complement(9092..9628)
                     /locus_tag="LK889_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5096663.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="LK889_002464"
     CDS             9937..11418
                     /locus_tag="LK889_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096664.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            11583..>11882
                     /locus_tag="LK889_002465"
     CDS             11583..>11882
                     /locus_tag="LK889_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5096665.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWT"
BASE COUNT         3664 a         2376 c         1943 g         3899 t
ORIGIN      
        1 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
       61 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
      121 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
      181 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
      241 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
      301 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
      361 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
      421 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
      481 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
      541 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
      601 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
      661 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
      721 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
      781 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
      841 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
      901 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
      961 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     1021 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     1081 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     1141 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     1201 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     1261 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     1321 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     1381 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     1441 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     1501 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     1561 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     1621 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     1681 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     1741 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     1801 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     1861 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     1921 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     1981 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     2041 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     2101 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     2161 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     2221 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     2281 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     2341 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     2401 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     2461 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     2521 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     2581 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     2641 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     2701 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     2761 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     2821 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     2881 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     2941 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     3001 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     3061 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     3121 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     3181 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     3241 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     3301 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     3361 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     3421 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcgctatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattccggct ctataattta
    11461 tgggactgat gaatcagaat tgattcttca gccccatttt tattttaggt attaaaaaac
    11521 atatcgttct ctagtatgat taaatcacga caaaataaac aactgagagg acgatatgct
    11581 atatgaatga ttctatcaaa aaaatgctgc gattaataga gaaagatttg atgattacag
    11641 aggtctctta cgagaccttt cagaagaaaa agacgttgat cgtcgatgct gttttctcac
    11701 ctgctcccca tacctgtaga aattgtggtt ctactgtggt agatggaaac gggaaagtaa
    11761 ttgtagtgaa aaatgggaag aaggaaacga ttgtccgctt tgaacaatac aatcacatgc
    11821 ctttggttat gcgcttaaaa aagcagcgct atacctgtaa aaactgtcga acccattgga
    11881 cg
//
LOCUS       DAFBQW010000078        11874 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFBQW010000078 DAFBQW010000000
VERSION     DAFBQW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.081"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..138)
                     /locus_tag="LK889_002466"
     CDS             complement(<1..138)
                     /locus_tag="LK889_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK5096666.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
     gene            complement(369..1499)
                     /locus_tag="LK889_002467"
     CDS             complement(369..1499)
                     /locus_tag="LK889_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5096667.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(2040..2246)
                     /locus_tag="LK889_002468"
     CDS             complement(2040..2246)
                     /locus_tag="LK889_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096668.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2262..2876)
                     /locus_tag="LK889_002469"
     CDS             complement(2262..2876)
                     /locus_tag="LK889_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBK5096669.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(3023..3706)
                     /locus_tag="LK889_002470"
     CDS             complement(3023..3706)
                     /locus_tag="LK889_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK5096670.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3815..4273)
                     /locus_tag="LK889_002471"
     CDS             complement(3815..4273)
                     /locus_tag="LK889_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK5096671.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4357..6120)
                     /locus_tag="LK889_002472"
     CDS             complement(4357..6120)
                     /locus_tag="LK889_002472"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK5096672.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6341..7801)
                     /locus_tag="LK889_002473"
     CDS             complement(6341..7801)
                     /locus_tag="LK889_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096673.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(8009..8935)
                     /gene="glsA"
                     /locus_tag="LK889_002474"
     CDS             complement(8009..8935)
                     /gene="glsA"
                     /locus_tag="LK889_002474"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK5096674.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8958..10385)
                     /locus_tag="LK889_002475"
     CDS             complement(8958..10385)
                     /locus_tag="LK889_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5096675.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10416..11630)
                     /locus_tag="LK889_002476"
     CDS             complement(10416..11630)
                     /locus_tag="LK889_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK5096676.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3591 a         2441 c         1958 g         3884 t
ORIGIN      
        1 cttcagttga tcagtatttc ttcggttttt aatcttatct ggtacacaat tgtggtatat
       61 gttagaaaca gttctcataa tttccttgaa tgttataaaa gtatctgtat aatgttcagt
      121 atattttgat gaacgcatat gtagtcctca tttcttggga gttttgagac tactatatgc
      181 gtttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      241 accacgggta taatatgatt ttttgagata ctttcattgc gaattcctct ttctaaaaga
      301 acctctcgct tgaaaaataa aagattgtac tttatgataa ttcaaactga gagtttctca
      361 gtttctcttt attgtttttc aggtcgatag agcgccaact cttcggcctg tatctttgaa
      421 gaaatattca acgtgattcc gattcctaaa gaaaggatca gataacttgt tcctccgtag
      481 ctaacaaatg gcaatggcac accagtcatt ggcatcagcc ccgcgatact tcccacgttc
      541 attatcgtct gaacgaatag caaagttccg aatcctaaaa gaaacaatcc agcttgctga
      601 tttttacagc gactgcttaa acaaaagatt ctcatgcata atgagaacaa taagaaaagc
      661 acacacaatg caccgattag cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt
      721 tctgtctctg gaagataccc tttttttgtg atactatttc ctaatccccg cccaaatatg
      781 ccaccattat atagtgcata aaacgaatgg gtcatttgat agcccgcacc atgactatcg
      841 ataaaagaat ctcttaaagt cgcgatcctt tcataagcat gattaaacat ctgtggaagc
      901 caacctttat ttcctaaata taataccccg cctgctgctc caatcagcaa tgcagaaaaa
      961 gtaacgatta gatagatccc ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac
     1021 gcaatcgaaa ggatcatcaa tgctccagca atctttggct gaaataaaat cagaaaagtt
     1081 atacctactg taatcaggaa tggttttttt agatcttttg ggctactatt tccgtcacga
     1141 aaaaaccagc ttaaataaaa aatcaaaaaa agatttgcca gttcagaagg ctggaattga
     1201 atgccaaaca gagagatcca acgttgtgcg ccgttgacag tgacaccgaa tatccctatt
     1261 cttactaata ctaaaaagaa aatcgataag gctaaaccgt atcctgctat tttagggtga
     1321 agtaaatagt gtagtttgac tgaacgagct aagaagatca cgctccaact caaaaatatg
     1381 aaaattagtt gacgcaataa tagacttttt gtattttcat cggcttgcaa taaacgataa
     1441 gaactggcac tgtatacttc taaaagaccg atcatggaca aagtcaaata aggacccaaa
     1501 atccaccaat cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg
     1561 tgaggagcga gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga
     1621 aaccttttcg aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat
     1681 acaacgtaaa gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt
     1741 tacccattgg cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac
     1801 ctaacaaggt tattactata ttctatttac gagttcatga tagacttttc ctaatgatta
     1861 ggcaatggaa tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa
     1921 aataacaaat aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt
     1981 gataattttt tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt
     2041 tatttgttaa agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta
     2101 ccatctaaag taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac
     2161 tgcccttgca gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc
     2221 gatggtttat agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc
     2281 gtgaaaattt ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat
     2341 tttcttggat cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc
     2401 tgtacttcta gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt
     2461 tcatataagg atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt
     2521 actgaatcag ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg
     2581 gctgctgttt tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct
     2641 cttgtctcaa gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc
     2701 aaggataaga tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc
     2761 actatatctt ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca
     2821 atcagcaact tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt
     2881 tcgccttctt tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat
     2941 gcttttttca tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa
     3001 gaatctgtgc cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt
     3061 tagtgatcaa ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt
     3121 catatgcttt ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat
     3181 cgacttgttc tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat
     3241 attctgtata gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa
     3301 gcgcttcctc aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt
     3361 ggacaagttc agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa
     3421 tgatttctgc ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca
     3481 cttctgaccc atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct
     3541 tgatttcatt gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg
     3601 cagccaaagc cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt
     3661 gacaagaaac gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag
     3721 taatgttata taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca
     3781 ataatgagct atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt
     3841 cgtatacgac cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt
     3901 taaaaaactc agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt
     3961 tccaagcatg ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga
     4021 tgatacaaac tggttcattt tttccattca ggaccacgtc taattgacca acttttggta
     4081 aaggctcatt ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt
     4141 ttacaactag ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac
     4201 ctaatttttc tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta
     4261 cttgctgata cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact
     4321 agtataaaca attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag
     4381 actatcttca acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg
     4441 aacgctatcc gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc
     4501 ataataacga ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat
     4561 gactaattcg tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt
     4621 tcctggattg atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt
     4681 tcctaattca agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt
     4741 ttgataggct ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt
     4801 aggaattcgg attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat
     4861 catatcctct tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg
     4921 aatgtcaaat gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa
     4981 aataatcact ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg
     5041 caagaaagta cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc
     5101 gaaagtgata gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc
     5161 ttggaatgtt ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc
     5221 tgtacttgtt tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc
     5281 agaagttact tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt
     5341 cattttatta tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac
     5401 aatcggatga tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt
     5461 tccattatcc acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt
     5521 accgttggtt tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat
     5581 agacatctca ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt
     5641 caatccttca aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt
     5701 tcctgattct ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt
     5761 agatgtgtgc cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct
     5821 cacttcacgg taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc
     5881 ataagactga tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg
     5941 aaacgcaatc gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct
     6001 gtccttttgc aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga
     6061 taattttttg acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat
     6121 aatacattct ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc
     6181 tattctacta cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag
     6241 cttgttatat aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca
     6301 attaaaaagc tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta
     6361 ataggtgaag ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg
     6421 gatagatctt tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa
     6481 ccactctttt tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa
     6541 taggatttat ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa
     6601 attgtttgac tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt
     6661 cactgttgat caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta
     6721 gttggatttg atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa
     6781 gttgttcttt ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc
     6841 acaacgtacg gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga
     6901 tttcagcgat actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa
     6961 aatttttata aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat
     7021 ttctactgtt gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc
     7081 ctttttgctc ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt
     7141 tcttatcttc ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc
     7201 ctcgtatgat aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta
     7261 cataatcaat caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt
     7321 cccaattgac ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa
     7381 tgatttcttt atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga
     7441 ttgtatatac tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata
     7501 taccattaaa ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg
     7561 tgagctcaga aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta
     7621 cttgttcttt tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg
     7681 ttggtgtagt cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa
     7741 ataattgttc acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca
     7801 ttcctgatac acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta
     7861 tttttgtttc ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg
     7921 acaaaagtca aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga
     7981 cttttacccc agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga
     8041 ctacatcttt caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc
     8101 tgaagacacc tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc
     8161 tttttgcagg tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag
     8221 ttgtcataat tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc
     8281 aaggagcaat tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga
     8341 cattgaggga gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca
     8401 tttgattacc tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag
     8461 aatgatagat ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg
     8521 acaagattcg ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa
     8581 tcgctcctgc attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca
     8641 tgatagaatt gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta
     8701 ttccgttgtc tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa
     8761 agcgcacatc ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca
     8821 tacctaattg tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg
     8881 tttgataata gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa
     8941 taaccagctt tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc
     9001 gtaaatggaa taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca
     9061 tgctgtccag ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa
     9121 gtcaaaagac ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag
     9181 gctctttcaa gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag
     9241 tacaacatca ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc
     9301 cccccgccgc ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc
     9361 atcaaatgag caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct
     9421 tcttctgctg ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg
     9481 atgacaccta aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca
     9541 atgtgtctta ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt
     9601 tgaggtactg tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata
     9661 accaatacaa acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga
     9721 ctggcagatg tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta
     9781 ggcaatttcg aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg
     9841 cctgaagcga cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca
     9901 gctacaaaac cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt
     9961 aaaatagccc agaaaataat caataggatt gccaatttca gatatttatt gttttgtata
    10021 gcaccaatac ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag
    10081 ccaactgtta tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat
    10141 gtgcgactga cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct
    10201 gctgcgcaca atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact
    10261 aagctgaagc ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg
    10321 gtcatagcca aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt
    10381 tgcatgaatc ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag
    10441 aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc
    10501 gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca
    10561 atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat
    10621 gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca
    10681 tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata
    10741 cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta
    10801 atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct
    10861 tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa
    10921 ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac
    10981 cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt
    11041 ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat
    11101 ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa
    11161 aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata
    11221 acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga
    11281 acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca
    11341 ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt
    11401 ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata
    11461 atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc
    11521 cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta
    11581 cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc
    11641 tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg
    11701 aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct
    11761 ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg
    11821 tttattttcg tcttttttta tttttaaaga tattttttca taaggaataa agaa
//
LOCUS       DAFBQW010000079        11623 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFBQW010000079 DAFBQW010000000
VERSION     DAFBQW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.082"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(286..2070)
                     /locus_tag="LK889_002477"
     CDS             complement(286..2070)
                     /locus_tag="LK889_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096677.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(2070..3821)
                     /locus_tag="LK889_002478"
     CDS             complement(2070..3821)
                     /locus_tag="LK889_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5096678.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(3963..4187)
                     /locus_tag="LK889_002479"
     CDS             complement(3963..4187)
                     /locus_tag="LK889_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBK5096679.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4438..6024
                     /locus_tag="LK889_002480"
     CDS             4438..6024
                     /locus_tag="LK889_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK5096680.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6090..7865)
                     /locus_tag="LK889_002481"
     CDS             complement(6090..7865)
                     /locus_tag="LK889_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBK5096681.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8078..8980)
                     /locus_tag="LK889_002482"
     CDS             complement(8078..8980)
                     /locus_tag="LK889_002482"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK5096682.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            9259..11604
                     /locus_tag="LK889_002483"
     CDS             9259..11604
                     /locus_tag="LK889_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK5096683.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         3545 a         2361 c         2141 g         3576 t
ORIGIN      
        1 caaaaagaaa aacccaaaaa gccgtccatg aaaccatggg agactcatca ggtttagctt
       61 gcatcaggca atcactatcc aagttgtatc gttttcttta tattatcgaa agcaaatgct
      121 tttgtcaacg ctttcttagt ggctattagg cacttctgac tagctgtcga atcttttttt
      181 aaatctggtg actacgacac taactctcac agatgaaaaa tatgagaagc tatgttttat
      241 tttcactcta gtgcttaggt caaaattcta ctgtcacaac atcttttata taccactgct
      301 ttgcagtttg tacatatctg catacaaacc attttgcgct agaagtgttt catgattccc
      361 gcgttctact atatgacctt tgtctaaaac taaaatcaat tctgcatctc gaatcgttga
      421 cagccgatgg gcaatcgcaa tcgttgtccg tccttgacgc atttttgcca atccttcttg
      481 gatcaatcct tctgtttcag tatcgatatt tgctgttgct tcatccaata ctagaatttt
      541 aggatcagtc acgattgttc gcgcaaaagt gatcagctgt cgttgtccac tagaatagct
      601 ggcaccatgt tcgatcactt ttgagtgata tccttttggt aattcatcga tgaatttgtt
      661 tgcttgaaca aactcagccg catggcgtat ctgttcgtct gtgatagctg gattcatcat
      721 ccgaatattt cctgcgatat ccccataaaa caagaaagca tcttgcaaga ccagccccat
      781 tttcttacgt aactcttcca ttggatattc tcgaatgtca tgatcatcaa tcaatatctg
      841 accttcataa aattcgtaaa accgcatcaa tacgttgatg atcgagcttt ttccgcttcc
      901 ggtgtgtccc actaatgcaa cggtctctcc cggattagca acaaaagaga tattgtttaa
      961 tacattgtgc tctccatcat aagaaaagct tacattgcga aactcaatct tacctgaagt
     1021 aatcatttga tctgctccag ggttttgttg tggtgcatat tcttccgtat ccataatctt
     1081 caagatacga ctaccagcaa ccatcccatc tgtgaagatg ctcaaaaagt ccatcatttg
     1141 agacattgga ttaaaaaatc cctggacata ggtcgtaaat gcatagatca gtccaacttc
     1201 tacgggagaa tgcaacgcat cgatcccaaa caaagtcaac accaacgcaa tcgccaatgc
     1261 atatagaaaa ttgatgatcg gacttaacag aatcgaattc gttttgatca tcgcataacg
     1321 cgtgtctaaa tactcattgt tcacttgttc aaattcttta gctaaacgag cttcttggcg
     1381 aaactgctgg ataatacgca tgccggaaat cgattcattc aatttcgtgt tcagctgact
     1441 taatttttcc cgcatatttc ggtaaatacg agaactgaat ttctggtaat accagatgac
     1501 gattcctaaa atcggcaaaa acaccaaagt ccacaaggca atttctccat tgatttgaaa
     1561 catggcaagg aatgaagaaa cgactgcaaa gatcccagtc aataccatca aaaagacgta
     1621 ccaaaattca aatagcgttt ctgtgtcatt cgtcactcga gaaacggttg atcctgccgg
     1681 tgtctgatca aaataacgca tacctagtgt atgcagcttt tcaaataatt ttaccctaat
     1741 atactgatag gtcttcaatg aggccatgga atacaaatac cattggaaaa accaaatgat
     1801 actttttact atgactccaa ataagtacat accagcaaaa aacaaaataa cttgtgtcgt
     1861 agccgactgt ttagccagat gatcatccat aaaagtttgg atgatccttg gcaataggac
     1921 attgatcaca gacaaagcaa acgcaaataa gatagcaacg atgaacgttc ttctaaaagg
     1981 tttggcgaac ttcatcagtc gtttgatgat ctttttctgt tctttaagtg gaatcgattt
     2041 tgtccattcc gattgataat tttcatccat caggcctcgc ctccttctat cttcgcttct
     2101 aattgctgtt tctcccacat ttgttcatac cagccgccta gtgcaagcag ctcatgatgg
     2161 gttcctcgtt caatgatttt cccttgatcg acaacaatga tttcttttgc atgcatcacg
     2221 ctacttaaac ggtgagctgc aatgatagtt gttttctctt ttcttatttc ttttaaatta
     2281 tgcaagatag cttcttctgt tttggcatct actgcagaga gtgcatcatc taatattaat
     2341 aattcaggat ttttgatcaa tgctcgagca atcgaaatcc tttgtttctg tccgccagat
     2401 aaagagactc cacgttctcc aactaaggta tcataacctt ttggtaattc cttgatttca
     2461 ttgttgataa atgctaaaga agccgcatgc tctacttctt cttctgaaag ttttggatta
     2521 gaaaaacgga tattatctcg aatggtcatc gaaaacaaga agtgatcctg aggcacatag
     2581 ccgattgatc ctaataaagc attcaatgaa tattctttga tcgaacgtcc gccaaattgg
     2641 attttccctt gataatcatc gtattcacgc atcagtaatt tgatgatcgt cgtttttccc
     2701 gcaccagttt tcccaacaat acctaatgtt tctccttctt ggatcgcaaa cttgatttgt
     2761 tctaatgctg gtgattcttc tttcggataa ttaaaagagg aaatagctac attcagctcg
     2821 ccttttgccg gggtctgcac tgcacccagt tgttccacga tatgcgtctt ttcatgcaaa
     2881 agttcattga cgcgatcata agaagcattt cctctttcca atacgttgaa caatcgacca
     2941 atcgcaaaca tcggccaaac aagcataccg atataactaa taaatgagac catctgcccg
     3001 atcgagatcg tgccttccaa gacatacttt ccacctaaaa taattgttgc aacataagac
     3061 agaccgataa tcaaggtaat gaatggatca aataaggagt ctaaaaaatt gactcgtttg
     3121 tttttaataa ttgcatcttc aatcttttct tcaaaatccg ctagatcttc tttttcttgt
     3181 ccaaatgttt taatgacttt gatcccagtg atactttctt gtgtcttatc attgatacta
     3241 gaaaaagctt cttgcgaatc acgaaaagca tcatggagtt ttgaccccag cactcgtgaa
     3301 gtgacggcca gcaaaggcag aggaatcagc gcaatcagtg ttaagcgcca atcaataaac
     3361 aaaatcatcg caatgatcgt tgttccgccc gtaatcactg aatctgcaaa ggtcaagata
     3421 ccagctcctg ctacattctg gatcgcattc aaatcattcg ttgcgtgagc catcaaatcc
     3481 ccagtccgat atttttggta aaagagattg tccatttctg taaaatgttt gaataactgc
     3541 tgccgaagtt ctttctccaa tcgagctgca cttccccaaa tattcgtccg ccagatataa
     3601 cgaaaaagat attgtaaaaa agcagcgata atcaatagga agatccaccc gaaaatgatc
     3661 tttattgata aatttttatc ggcgatctca tcaatgatga tcccaatcac ttttggagga
     3721 actaactgta ccaaggccac tgcgaatagc gagaaaaccc caattaaata atgtttcttt
     3781 tcctgacgaa aaaaccagcc taattttcta aaaatcgaca ttttactcct cctttgtctt
     3841 tatcaagagc ctaggattgt ctgtgcttgt cttttttgct gaataatctg aatagtctaa
     3901 caaattacga aataaaaaac ggactgaaaa aatcagcccg taaaaagtcg ttgggtcaga
     3961 acctattttt tcccttggtt cttcatttgc tgcatcatct ggcgaatttt cttctcagat
     4021 ggtttttgcc ccattgacat catcattgat cgcaacatgt cctcattaac gggaggattt
     4081 ttttctaaat agtctttcat atatttacgc gctaggaaaa agccacctac tgcgccgatt
     4141 agcaaagcaa taatagcaat aagtacgaca atacctgttg ataacatata aatctctcct
     4201 ttctatttcc gaattgctct caatgactta ttttactaaa gtttattgaa aaaagaaagg
     4261 cgtattcttt agtttcatgc aaaaaatagt ggttttttta gaggagaaca aaatttcttt
     4321 cgtcatctag caagacgcgc catgaaaaaa aattaggcgt accgaatctt tttctttaat
     4381 tttttctttt tgaggtgtag aatgtaatcg tagataaata ggaaaggtgg ttttttcttg
     4441 aataatcaat ccaataaaca atttcataag catcgactta ttgaacatac aaatggttta
     4501 tctttagctg aagtaaatgg aacagttgat gtaccaaagg ggaaaagttt ttttaaaaca
     4561 cttcttgctt actctggacc tggtgcgtta gtagcagttg ggtatatgga cccgggtaac
     4621 tggtctactt cgatcaccgg aggacaaaat ttccaatatt tattgatgtc agttattttg
     4681 atgtctagtc tgattgctat gcttttacag tatatggcag caaaattagg aatcgtgagc
     4741 caaatggatt tggcacaggc aatccgagca cgcacaagta agacattagg tgtcatttta
     4801 tggatcttga ctgaattagc cattatggca acagatattg cagaagtcat tggtgcagca
     4861 atcgccctct acttactttt tgatattcca ttggtgattg ccgtctttat tactgtatta
     4921 gatgttttcg ttctcttact actgacaaaa gtcgggtttc gtaagatcga agcactggtt
     4981 gtctgcttaa ttttcgtcat tttattcgtt tttgtctatc aagttggctt gtctaaccca
     5041 gattggcaca gtttatttgc tggctttatc cctaacagtc atacattctc agactcaccg
     5101 gtcatcggcg gcgaaactcc gctgactgga gctttaggga ttattggtgc aacagtcatg
     5161 ccgcataact tatatcttca ttcagcagtt tcacaaactc ggaagatcga tcaccaaaac
     5221 gaagaagatg tagcacaagc tgtacgtttt tctacttggg attctaatat ccagttgtca
     5281 atggcttttc tagtcaatgc actgctattg atcatgggtg tcgctgtctt caaaaccggt
     5341 tctgtccaag atccttcctt ttttggtctg tatgaagctt tatctgatcc atcagctatg
     5401 agcaatggta ttttgattgc tgtagcaaaa tctggtatct tgtccacttt atttgctgta
     5461 gcacttcttg cttctggcca gaactctact attactggta cgttgaccgg tcaagtcatc
     5521 atggaaggat tcatccatat gcgtatcccg atttggctaa gaagactggt cacacgattg
     5581 ctttctgtga ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa
     5641 gaacatgccg ctttgaataa cttaatgaat gaatcccagg tgttcttggc ttttgcgttg
     5701 ccgttttcga tgattccttt actgatgatg accaacagcc aagcggagat gggtagccga
     5761 tttaaaaatt cattcatcgt aaaacttctt ggctggttct ctgtgattag tctaacttac
     5821 ttgaacttac gaggattacc aggacagatc gaaagtttct tcggcgagca agccacttcc
     5881 gctcaactag cattggctga tcacatcgct tatgtaatca ttgttcttgt ccttctcctg
     5941 ttggcatgga cgattggtga tttatataaa ggaaacaaaa aatatgaaca gcttttgcag
     6001 gaaacggtag aagaaatcga ataaaaactg atttttccac acaaagaccc ttgatagtta
     6061 ggttaaaaat ctagctacca agggtctttt tatttagaag ataaagcttt tttcagattt
     6121 aatttactga aaactagcat gcttgttact acgatgaatt gcgttaatgg cacgcctgca
     6181 ccagtgtaga cacttaaaaa atagtccttt ttttctggcg cataagaaca aaaccatgag
     6241 acaccgaaaa gaaaaaccaa ttgtgtcagt attaatccgg tgaccacaat caaagaggaa
     6301 ctataatagg cacgatcagc ttttgcaaat acggttccta gtatcataag taatatccct
     6361 gctattgaag caatcagcac gggaatagcc agaacttggt aaatttttat gatgacattc
     6421 gcgacttttg ctgattttgt taattggaaa ttaggtgttc tcggttcatt actatcgatc
     6481 caattctcca ttagcggaag gtttaataat tcttgagcct gtttgacttg ttcttgcgtt
     6541 ccgaaactat aatttcctcc ttgatggaaa tctttatatc gccatgcata agcgtatcct
     6601 gattgaagga aatcgaatac gattggcaga tcactcatcg tctttccatc tcctgatccc
     6661 atcagataga actctttttt ctttttgatt gttcccttgt gataggcttc ttctaattct
     6721 tggttgactt ttttccaaaa atcattggtt tcttttgcat tgttttggta ataaccagat
     6781 tcgcttacca catctcgcaa tgcccaaaag atgatatctc cgggtaattc ttttactttt
     6841 ccaccttgag tccacgcgtg agtcgtatat aattcgtcaa tacgggtaga aacagtatgg
     6901 aatgtaggag atgcggctaa tgctttattt agtgcagcat gtgagaccca tatctcactg
     6961 ttttctttat tcaagttcgt ttcatcatct attcggatca gttgtttcat caaacgtcca
     7021 aattctccac tggaacggtc attgatggtt gcaataccgt atgttgtctc atttttatat
     7081 tcgatccatt gatagacgat ggaaaagcca actataggaa gtaaaaacaa tgaaagtttc
     7141 agccattccc gtttcgagag aagaaagagt cgttttttta acttctttac ttctttgaaa
     7201 gaaaagtctt gccctagcaa gatctggacg aaactgatac ccaaagctac tacagtaaaa
     7261 ggaagaagcc agatagaatc ttcgcgaatg aaccaaaaga aaggaaaagc gacagaaagc
     7321 cctaaaaacc atggcagcaa ttttctgcta gaaccgtatt tgcggtaata aatagctaat
     7381 aaacaagcga taatgatctc aacagccgga atcacgaggg cattacgata tgttcgagtg
     7441 gaatattcat gtgtaaaact aatcggagaa aacaaaaaat aaaaatagat acttaaaaga
     7501 agatacttgt tcttcacaat cggtttaaac gcgagtgcaa ccaatatact ggcaaaaata
     7561 ctgaacatgc cgaacaaaac tgcataagaa agatgcagtt tatgtgcaag tactaaaaat
     7621 aagctgtagg agattccttt ggaaagtgtt gtagtcgtat actcccctag ccatttaccg
     7681 tccaaaatat tttgagcata gcgaatcagc agctggtcat cataggttgc cgacatgtct
     7741 gttccataga ttgctttact atataaatac aaacgaataa ttgtaaataa tagggctatg
     7801 ccaagaataa agtatgtatg tttctttttt agttggttga tcaagggttt taaagtgttc
     7861 agcatctttt cgtccttcca atcacgtttt gccacttgct tgtgaaactt ttcgtttttt
     7921 tacttttcta ccagtatacc ttgtttgtct aaatttaaaa agagggggga gaagatgcat
     7981 gatgaaaaag actgcaaaaa gtactccttt gtttcttaaa caaaaaagga caaggcaatg
     8041 tgcctggtcc ttttttgctg ctgtcagata gaaaatctta gagtatcact tcttctgcta
     8101 ataactcagg aaacacttct ggatcgaatg tttgcgcttt gaacatggcg atttcttttc
     8161 catatggtgc gtaagctttc tttttatctt tgcctacata gggagtttcc aagattttcg
     8221 aaatattgtc aaacattgga tcatggacga tcttattcag cgtgtcgaat ccgattgttc
     8281 caaaaccaat attggcgtgc cggtctttat gcgaaccttg cggattttta gaatcattga
     8341 tatggagtac tttaagccga tctaacccga tgatcttatc aaactcttcc attacacctg
     8401 aaaagtcttc tttaatatta taaccggcat cattcgtatg acaggtgtca aaagtaacgg
     8461 ataatttttc attcaatttt actccatcga tgatttgcgc taattcctca aatgttcgac
     8521 cgatttccgt acctttacct gccattgtct ccaaagcgat ttgtgccgtc tgttcttttg
     8581 tcaaaacttc attcaagcct tcaataattc tagcaattcc ggcttctgtt cctgccccta
     8641 catgagcacc tgggtgaagt gtaatttgag tagcgcctaa agcttccgca cgaacaatct
     8701 cttctctcag aaattggacg gcaaaaggaa acatttctgg tttgatcgta ttccccaaat
     8761 taatgatata aggggcatga acgacgatat ttgttagacc tttttctgcc atgtacgctt
     8821 ttccggcatc aatattcatc tcttcgatgg cttttcttct agtgttttga ggggcgcctg
     8881 tatagatcat aaaagtcgaa gatccataac tggctgcttc ttctgctgct cctaacagca
     8941 ttttctttcc gctcatgctg acgtgtgagc ctaataacat ggatggattc ctccttgaaa
     9001 ggcaattgtt actaacaatt cctatatatt ggtaagatga tatacaaaga aaatgattga
     9061 tttttctttg ccctgttctc catagtaact gaaattttct ctaaagaaaa gcttaagaac
     9121 tcttttataa taaagatcat tttctcacgt tttaactctt ttaaaaaaga aattcctgcc
     9181 aaaaacagcc ttgtctgttt ttttgttcta ttatttgcta taattagcga aataaaacca
     9241 ttagttgagg tgaccctttt gccacgcaaa aatactcgaa aaaaacagaa tagaagaaaa
     9301 aaagaaaaat ggtttgttcc taagattatt tttagagttt ttcaatcatt gactgttttt
     9361 attactgttc ttcttttgat gttcgcagct ctaggtattg gaataggcgc tggctatttt
     9421 gcctatctcg tcgaagacac acaattgccg actaaaaaag cactgcagac agaactagga
     9481 aacattacag aaacgtcaaa gattgtttat gcagataata cagagatatc aaaaatccag
     9541 acagatctga tgcgcacgac tatttcatct gataagattt cacctttatt gaaaactgca
     9601 attatcagca cagaggatga atacttcgat aaacaccaag gttacgttcc taaagctgtt
     9661 ttacgagcat tagtatctga agctactggt attggctctt ctggtggatc aactttgaca
     9721 cagcaattag tcaaacagca gatccttaca gatgaaacaa cattcaaacg taaagcaaac
     9781 gagatcattc tttctgcaca agtagaaaaa tacttttcaa aagatgagat cattgctacg
     9841 tatttgaatg tctcaccatt tggtcgtaat aataagggac aaaacattgc tggagtccag
     9901 gaagctgcaa aaggaatctt tggtgtagat gcaaaagatg tgacgctgcc gcaagctgct
     9961 tatatcgctg gattacccca aagcccgatc acgtatagtc catacacgaa cacaggcgcg
    10021 ctaaaagaag atctttctgc cggccttgcc agaaaagatt ttgttttatt cagtatgtat
    10081 cgtgaaggcc aaattacgaa agaacaatac gaagaagcaa aagcctatga ccttacaaaa
    10141 gactttctac cacaacaaat cgcagaacag actgacagag aattcttgta ttatactgtg
    10201 atgaatgaag caacaagaat cattgcacaa caattggctg aaaaagacaa cgctgatatg
    10261 tctgattcat cggtcagtga cgcttattat caaaaggcac agcaaacgat ccaaaataaa
    10321 ggatatacaa tccattcgac aatcgataaa gatgtctatg ctgcattgca aaatggcgtt
    10381 gaaaattatg gatacatgtt agatgatggt gctggcagtc aagtagaaac cggaaatgta
    10441 ttgatggata acagaactgg ccgcatctac ggattcgttg gcggacggaa ttacagtcag
    10501 aatcaaaata accatgcctt cgatacagaa cgtcaagctg gctcatcaat caaaccagtt
    10561 cttgtttatg gacctgcaat cgacatgggg cttatcggat cagaatcccg tgtatcagat
    10621 tatgcaacaa cttggcaaga aggagaaaat gctggcgaga aaatcgtcaa tgctactaac
    10681 gaaggatcga acaccttcca aaccgtccgt gagtcactag aatggtccaa caatatcccg
    10741 gcttatcact tatatcaaga tgtgttgaat tccggtggat cgaaacaata tgcttacgag
    10801 cactacttgg caaagatgaa ctacccggca aatgacaatt ggggcgttga atctgctcct
    10861 ttaggtacag tagatgtcac gacgcttcaa cagaccaacg gatttcaggc tttagcaaac
    10921 ggcggcgtct atgaagaagg ctatatcatc gattccatca cagataacgc aggaaatgta
    10981 atttataaac acgaaagcaa ctctgtccgg atctattcgg aagcaacggc atcgatcatg
    11041 aatgacatga tgcgtagtgt gatcaatgca aaaatcacta caccattcaa agatgccatc
    11101 tcaagcttaa acggaaatct aggaaaagca gattgggtcg gaaaaactgg ttctaccaat
    11161 gaataccgag attcatggct aattgtctct actccgtcta ttaccatcag tagctgggca
    11221 gggcacgatg acaatactgg aatggacagc aaagcaagga tacgttcggc taattatctt
    11281 gccaatctga tcaatcaagc ttaccaagca aaacctgaca tttttggcac tgatgagaaa
    11341 ttcgaattat catctgatgt gatgaagaaa aaagtatctg gatttactgg tcaaacacct
    11401 ggaaaagtta ctgtcaataa aaaatccatc caaacaccag gaaaaacagt tgaatcgcta
    11461 tgggcgaaaa atggaccgaa gaaaagcgcc tttaaatttg gcgttggcgg taccgatgaa
    11521 aactatacag attactggaa caccgcttca gcttatgcaa gagcgaatca agcaactaaa
    11581 gaatcagatg agaaagatga ttgatttaat agaatacaca gta
//
LOCUS       DAFBQW010000080        11384 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFBQW010000080 DAFBQW010000000
VERSION     DAFBQW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.083"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1655)
                     /locus_tag="LK889_002484"
     CDS             complement(<1..1655)
                     /locus_tag="LK889_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5096684.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAV"
     gene            complement(1708..2379)
                     /locus_tag="LK889_002485"
     CDS             complement(1708..2379)
                     /locus_tag="LK889_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK5096685.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(2461..3627)
                     /locus_tag="LK889_002486"
     CDS             complement(2461..3627)
                     /locus_tag="LK889_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBK5096686.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(3633..4322)
                     /locus_tag="LK889_002487"
     CDS             complement(3633..4322)
                     /locus_tag="LK889_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBK5096687.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(4449..4688)
                     /locus_tag="LK889_002488"
     CDS             complement(4449..4688)
                     /locus_tag="LK889_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096688.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(4755..4994)
                     /locus_tag="LK889_002489"
     CDS             complement(4755..4994)
                     /locus_tag="LK889_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096689.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(5108..5449)
                     /locus_tag="LK889_002490"
     CDS             complement(5108..5449)
                     /locus_tag="LK889_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK5096690.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(5436..5765)
                     /locus_tag="LK889_002491"
     CDS             complement(5436..5765)
                     /locus_tag="LK889_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096691.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(6356..6688)
                     /locus_tag="LK889_002492"
     CDS             complement(6356..6688)
                     /locus_tag="LK889_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK5096692.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(7525..8610)
                     /locus_tag="LK889_002493"
     CDS             complement(7525..8610)
                     /locus_tag="LK889_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5096693.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(8930..9337)
                     /locus_tag="LK889_002494"
     CDS             complement(8930..9337)
                     /locus_tag="LK889_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096694.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(9356..10174)
                     /locus_tag="LK889_002495"
     CDS             complement(9356..10174)
                     /locus_tag="LK889_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5096695.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(10400..10693)
                     /locus_tag="LK889_002496"
     CDS             complement(10400..10693)
                     /locus_tag="LK889_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBK5096696.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
     gene            complement(10705..10884)
                     /locus_tag="LK889_002497"
     CDS             complement(10705..10884)
                     /locus_tag="LK889_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096697.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQLISMNPFLFLQIGVDTRI
                     MRVLNYYCKRNNKRK"
     gene            complement(10881..11231)
                     /locus_tag="LK889_002498"
     CDS             complement(10881..11231)
                     /locus_tag="LK889_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096698.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(11224..>11384)
                     /locus_tag="LK889_002499"
     CDS             complement(11224..>11384)
                     /locus_tag="LK889_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HBK5096699.1"
                     /translation="EENLDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAG
                     GNGGIKND"
BASE COUNT         3388 a         2191 c         1618 g         4187 t
ORIGIN      
        1 actgcttctt tgacaccatc tttatctgta tcagtaagtc caccgtagta cttaccttct
       61 gcattcttag ctgcaaattc tgcgccttca agcggtgttg ctttgatacc tgttccaaat
      121 aggccgctat cgacttttaa gaattttgcg ccataagtca caacgtcagc tttcgttttt
      181 tcgtggtgtt cttcctgatt aaatggatta ttgttataaa cgaaatccac gctatttgtt
      241 aatggatttg cgatttcagc actatcattc acaaacatgt tataagtcat ttttagatct
      301 tgaccatgta aattcgcaac tgtatcactt acttgtccat tatttacgat aaaatcgatc
      361 acaaaaccac tatcagaatt ttcgatttta tagtcagtat tttcttttaa tacgactgaa
      421 ccatcattat ttgttacttt aatatctgct ttttcagcaa cataagaaag tcctttacct
      481 gctgtgtcgc cccatttaaa tacactgtaa ggagaagttt cactttttac accatttact
      541 gtaatcccat ctgttccacc tgcgatatct gtagggatat gaattgtacc ttgataattc
      601 actgcgtcac caatttggaa agaatgttct tctcctgttc catttgtcac tgttttatcg
      661 atcactggtg ctttatagtt cacgtaggaa gcttttaaat cacttgtcac gccgtcattc
      721 gtaatttcaa aggttaactt attgtgcgca tcgttgcgag cgtctgcgcc taacaaatat
      781 tggtctgctg ttggttcttg atctgatcca actacaactt ctgaaggaac ttccacaaag
      841 tagtattttc ctaacgtcaa atcagttgca gtcagctttc cttgatcatc cgatacaagc
      901 tctcctaatt ttgttccttt tccaggttcg cccttgtata actcaaattt tgcccctttt
      961 aaaggtgttt gtttgctttg agctgtgcca tcgtcaccaa atttcgttaa ttcaaaactc
     1021 aacttagaaa caatgttctt tggatacaaa tgaatatctt ttaaaaatcc tgatcctgtg
     1081 ttgtctgtca ttggcgcaat cacaaccatt ggttttgctt tttgtgtgac taatccagca
     1141 gcggatttac tttcataaac cacatataca ttccctttgg aattgacata ggctggttga
     1201 tcagcaaaag tgatctcacc attttcatct acagctgaag taattttatt ggttgcattc
     1261 ttaacgtact cactgttaga tcccttgtct tctacatctt taacgatttc gtccactttc
     1321 gcgttggtta gatcagaatc ttcagtcggt aatacttgat ccgtaatatt tcctaacgta
     1381 aactcaacat caccataatc tgccttgttg tatttcgtta cacctgtagg atattgatcg
     1441 tgcgtataac catcattttt gattccgtca atatcaacgt tcaattgcgt actttgatcg
     1501 tacattaatt tatgaaccgt gatattgacc ctttctggtg gcgttttttg cgtgacatct
     1561 tctgcataag ctcgataccc ctgaaagcag cctgcaatga ttcctaacat tccaacaatc
     1621 attccagctt tttttacata ccgatttatt gccatttttt actctcctca tttcatattt
     1681 caacgactcg cgttgattaa attcttgtta ttgtagctct ccctgtacca cgtatctttc
     1741 ttttccttgt gacgtataaa cacaagtaaa caacgttaat aagggtttct ctcctttctt
     1801 tggtaaagac aagaaagagt tctcttcagg aacgccatcg attaatttaa agtactcagg
     1861 tacgataaat ttacgcgtta ccttgtaacg atagacgcca tcgttactaa ctaagtccat
     1921 cacctcccct aatgacaact tttgaatatc agaaaataaa acacctggca tttccatgtt
     1981 atgacctgct aggacatagt ttcctttacc catttcggca tcttcataaa aataagtagc
     2041 cgctccaaga ctcaaagtgt attgatttgc ccctttaaaa attggcaatg ctaaaccaag
     2101 tgcaggaatc tgaatttgtg caaatacttg tttattgacg cccatttcga ttgcttcttt
     2161 ttgtatggac aacatttgcc ccaaagacat atcattattg acgttcgttg catcctcctt
     2221 ataactcttt gcgtgtgcat tgatttgatc tacgggcatc tttttttcta tattttgctt
     2281 aaacacctct ccttcatgtt tttggtagct aacatagcca aacacactgc aaacgacgat
     2341 taaagcgact cctaaaaaac caaataacca ttttttcatc catctgcctc ctttcagaca
     2401 aaacaaaaaa cactttttca caacgaaaaa gtgttttctc ttattactat tctatttcat
     2461 ttatctggtg gttttatatt ggtgtgtagc cattgctaac attaagaata caattggcac
     2521 ccaactgatt gtcgttaatg tttcttccat gaacgattga cacatgatcg ctaaaaaagc
     2581 ccacaagaat gttagttgtc catttgtcag ctcacgtttt cttacgagca accataagaa
     2641 actaagcaaa agaataatcg atagaacaaa accaatcaat ccatagtgat tgacgagtgc
     2701 gtgatacgta ttatgattac ctagaatctg atttcctcta tggaaaaaga aaggtttcat
     2761 ccctacaaat atttgtcctg ttttttcgcc taatgtttca tagaatttgc gccaaatttc
     2821 ttctcgtcct gtaaataagt gaactttgtc tgacattgca gcaaaataag aaatcggtaa
     2881 agctagaacc gaaactgctg catacatcca aaaggacaag tgtgtatttc gaaaaacctt
     2941 cttaggtaaa atttctaaga taatgaagat aacaagcgca tataatgctg ctttcgattg
     3001 gcaaacccaa atcgttgccc cagcaagaat caacgctggt aatgatagca atcgcatgta
     3061 ccattttcca aatgaactag caaatcctgt gataagaagg gctaatgtca ttaaagaact
     3121 acctatcgta tttgtattga tccaaatatc tgataatttg ttcgatggat tccaaatact
     3181 ttgtccttct ggtactggta tctcagtaaa tactcgataa accgttacga gtaaaaagac
     3241 atagaatact acaagataca agctacgatc aattacagta aatttcgtcc attttacaat
     3301 caaaataata gcaataattg tcggaacgat cagatcacca tagaaagtat ctcctcggta
     3361 gcttctaaaa aaagaaaaga aaaggtaaaa aagaatgcct gctgcaatga agaatagatc
     3421 gtttctcttt accttgtaca gattcaaaag aaacaaacag cctacgaaaa acgtcactaa
     3481 ctgtgcagca ttatcataaa taaataatat cgtaggagga tttaacccca ccgtccatat
     3541 taaatataga atggatacca acagtaacag aaaaatggca ctcattttat tgatcaattt
     3601 ttctacaagt tcttgaatgt gactcacttc ccttatttat tgattttttc tacattgact
     3661 aattcacact ggatcacatc gcactttttc atattatttg cttgaaaggc taacagtgtg
     3721 agtagtggtt ttttacctgc ttttggcaag gataaaaagg aattgttttc cttagattga
     3781 actacctggc tgtaatcaga aatttttcct cttttggaga cttcatactg atacttgtaa
     3841 tgagcatcga ttagatcaat ttcttctcct tcagataaat cagtcatatc ggataataaa
     3901 acgcttggtg tatctacgtt ataaccagca agaatataat ttccatttcc catttcagta
     3961 tctagataat aagtagaagc tactcctaaa ctaagcgtgt cgctattcgc tccttgaaaa
     4021 acagggagaa taaatcctgt agaagaaaaa acaagttttc cagcaatttc acctttgaga
     4081 tccttttcta atgcctgttt ttgattagta atcagttgtc tcttagaaat atccgttaaa
     4141 ctggttaaat tatcttgata ggttttagcc tgttcctgaa tatggattgt ttttgttaca
     4201 ccatctactt tttcagcgat tttgtccttt tgataacctt gataagcaaa ataactaaaa
     4261 agaagaaaaa caatgattac gcccacgtaa ggattaaata aagttttatt gtttttattc
     4321 atctatctac cttctatata tgaattaatt tacctacatc taaccaagat aaaccatgtt
     4381 aaattttact attcttttta ttcaataaca atgccacatc ataattgatc attttcatat
     4441 cataatcgtc aagaaacttc taataatagt tggcaattgg aacctgtttt tacataagtt
     4501 cgcctcaact gttcaaagtt tttcattgaa cctaacaaac aagctttctc ttttcctgtt
     4561 atcttcatat cattgatata ttgcatgaca gataatcttt taaggtgttt ttgtttcatc
     4621 atttttagca acttttcttc catttctttc gacacttttt ttctgttttc gatttcttca
     4681 taattcatga gttttcctac ctttcttgtt ttttgcaaaa ataaaaaagc ctatgatcac
     4741 atacgctcta cacttcagtc ttgggttatc tttttctttc gtttaattac tggccgtgct
     4801 ggtttcaaat ccttgattcc attatgggtt ctccaataat cgaattcttt tcgtaatctt
     4861 tctttttcca tatctgttcc tttagacagg gatttgaata catctttagg tgaataacca
     4921 cgagctaaca gctgtacata ccttcctttt gtcaaagtat ccatatccat cgcctctaaa
     4981 actggtcgcg ccatctaatt ttcctcctaa ataaattcct tactcaatgc ctaaggtaat
     5041 actacctttt tattattcgt cgtgactcct tctaagcctt tatctgtaaa atttattcct
     5101 ccagttttta ttcttcttta tccgctacaa attcaatgat ttcaacgctg acaatataat
     5161 cactcaaata tgaccaagta tcttcaacag ataattcgtc tccattatca tccgtaagcg
     5221 tcattttaaa ccattcatta ccttcatcat cttcaggtag caagccgaat ccaattggat
     5281 tatcagtcaa tgtttgatcg tataaatcat cctcgtctaa tgctaaaaca tctaatcctt
     5341 tacaatttcc gcctcttcta aacgtcacaa atcctttgta atcccaacgt tggaaagaaa
     5401 ccttaatcgt atgtgtgccc aatcgtatat tcggatcata atttttcatt tgttgtaacc
     5461 tcctcataat cagttagtac tataaaggta tctttgtttt tatccaaaaa atctaataaa
     5521 tcttttgtag aagatatctc ttcatcaact tcaccataat aaataccttc gataattgtt
     5581 gctatatctg aaaaactaat ttttactgga catttatcgt ctaccgcatt caataccgac
     5641 aaaattgctt ctttttctgc aatctctcgt ttgaatttct ttggagctaa tcgcatgaca
     5701 ctcaagtcat tttcaagctt ttcaatccat gcagttttac tcattttcgc aaatttttga
     5761 atcattcctc taccaccttt gctatctact aaattagttt tacttcataa cgaatatctt
     5821 tatcgttttt cgttcactca aaaatatata aaataaatcg actcgtttat ggcgtccgaa
     5881 acgagaaacc ctagtagaca gcggtaaatg ctcgattgat ccaatgatca acgtcctcgc
     5941 aagtatctac atgtactgca gcacacgaca caatttcttg tgcgtcctcc ccattacgag
     6001 aagacctact ccttattcca tgagtttgcg tcaaagccta gatcccttag tagctgctca
     6061 tgattaatct aggcgctatt ctaacttgtc taacttctaa attccctctt gtttactctg
     6121 tgcatattat ttacacaaat tcatttcacc actatccaaa caagggcatt tactcgattg
     6181 accaattttt tacgttacca atcgtaaaaa cctaccccgt cactccccga agggaattgt
     6241 tgatagatca agtctactcc ttatttttct agtgattcta agttccttta gaagtctcga
     6301 caatattttc aagcaagcga aaaacgataa aatacaagtt atgagaatga tcaattcagc
     6361 tgattaaacg ccccatccct tttgcggtac aacgcaagac tccatttttt cttcgaccaa
     6421 tcagtacacc gttttggtca taaacatgtt tgattgttgg atatagctct tttaaaggga
     6481 taggatcttt taaaattatt tgtttttgtt tcccaatgta ataaatcatt tcttctgaga
     6541 ctttgtcttt ctggattata tatttcttac actgcacaac ggttttactt tgaccagttg
     6601 atagtttctt cgtgatcttt tctgtagcgg tacaccgttt accgtagtga caaatatctt
     6661 cgagactcaa aattcctgta tatgacataa aataatccac ccacttactt taaatatttt
     6721 taacaagttt ttcttgttat ttttcaaaaa tagagaattg attttcgttc atgacaaaca
     6781 aaaaagaggg aacgcattca aacatgcgtt ccctctaatt tcagcgttcg tcatacggca
     6841 aagagcccca cggacatttt tataacatta tgcttaggca tagattcatc gtaatatcac
     6901 gtatttttaa ccttaacaca gccaaaaaaa gtggtatcac ttttgaatat ctgttatact
     6961 aaatccgtta cgggtaatac cgtaacactg aaaggtaaac gcattaaggg gtatagactt
     7021 aatgccacct gtggacgtcg acgaaggttg gtagccttcc gagtctcaca gggcctttct
     7081 tttttttgta ttttcaattg tccaaattta tcgtccaaaa gtgcagaacg aaaatcaatt
     7141 catcacaagc atagcagaca attaaattta aaacaactaa atttatacaa aaaagtgtaa
     7201 ttgaatatta gcagcactgc ctgaaaagaa gtaatatcag gcttttcagc ttgggtaaat
     7261 aagtgaatta cctctcttca tgaattatta taactcaaaa aaatacatat gcaaacacta
     7321 gttccctgtt tttttgaatg aaaaataact aaaatatcta acgattatga cattaaaacc
     7381 tacgtttgtg attgttttca taaactttta atctaaaaac tccggaataa taactattta
     7441 tttgtaacat ttgtaataaa attaaaattg taaaaaagtt ctaatttaaa attgatcatt
     7501 tttatagtgt tcgcttttta tcaactactc tagccaattt tccgtactaa ctgttggtaa
     7561 ttcgttaaat gtttctcttt gttcgtttgc aagctttgcc gcaatagctt tttcaataaa
     7621 ccagtttttt acatacacaa aaatcaaatt ctgcaaatta tttactttct cagtcttttg
     7681 tttttgataa gctttcagca acgtcatata taatccttta tccgcatcaa ttccgtaaga
     7741 ttcaagttct tcatacacaa ctaccgtctt ctcaagttcc tcggctttat gttttgcttt
     7801 gtgtattgtg gtgacggttt gttgggcttc ttcgtaagtg ttagaaaatg tttttatcaa
     7861 gcttaatacc ttttctggta caaatgtgtt tatgctgtct tctttcatga tttcaacgaa
     7921 gttatctagc aaaacttgat tttgtaattg gtctgtttca gtgtctttta agtgtctttt
     7981 agtgtctaaa gatttattaa tataataatc ttgtgcgatt tccgcatttc cagtgttatc
     8041 aagggtttca gctgtttttt ctcgacgtgc gatttccgca tttccagtgt tatcaagggt
     8101 tttagcagtt tcctctcgac gtgcgatttc cgcattccga gaagaggttg atttatcaat
     8161 agtttcgctt aaatcgtcat cgaaagaatt ttgaataaaa agatcatcgg cacttatttc
     8221 aggttgaagt aaatagtatt tatttttttc attttttttc gtttttgggt taaattctcc
     8281 ttttacgact gaaatcaaat tatatgtttc taactcgttt aaaatggttt ttactttgcc
     8341 ttggtagcaa tttaatttgt ccattaggtc tgcttgtgta aattccaaat ataatagacc
     8401 atcatcgtcg atccattttt catgtaaggc tctacgaaat tcatttttaa aaagcatata
     8461 ggctattttt gagctgtctt tcattttaga gtatttagaa ttctgaatta aaaaactagg
     8521 aaatttataa tatttttcaa tataaacatt cgttgcttta tatccccaat ttttaattat
     8581 cattttttga gattgatttt ctgtagacat taaatttctc cttcttctta gaaacttgta
     8641 tatattcagg attttcaaca agtgaactat accatgtatt aagggtgaat aaaacacttt
     8701 cagtatttaa ggtataagtt atatattaaa aaaataagta taagttatac tttaaataca
     8761 aataatgtat aacttatact ttaaataata attttataat gttgatttaa tcgcaaattt
     8821 acacgttgtt tattaagtta taccttaaat atttaatgta taacttatac tttaatattc
     8881 atttatctag tataacttat actttaattg aataccgcat gaaaaaaaat tattttggta
     8941 atttttcaaa ctcttcttgt atttcttctc ttatgtgttc ttcttttgct actcctagtc
     9001 tatgctggat atatgagtct gctaatgtat taacaatttt ttcgaaagaa attctccctt
     9061 tttcactttg taactccgtc atatagtctt gtaaaattga gagtttgcta tttaatgccg
     9121 gagagatctt tttaggaact gataccttgt ttcttttatc aggaattgaa actggtcggc
     9181 ctcttttttt caatgtaata tttcttgtgt tactcaatgc actattgcta aagttatatt
     9241 caccgcttat tttcacatct tggcgtttgt tattgggtaa taatggctca aattcaccat
     9301 tctccatcat tttaggaata tcaaaatctt taggcattac ttatgcaact ccttattatt
     9361 tatttaaaaa ctcttgtcta gcatctagtt ctgccaaaat atttataaat acttggtgtg
     9421 ctttataatc ccattgatca aaatttccat ttttgtattt tcttaaatgt ataccaattt
     9481 tagaatacct tctcaatctt ttttgataat gaatgagatt tttggatacc acggttccgt
     9541 atttttcaat tgcttcttct aggatttctt catcaaaatc atcatttggt tctaccatac
     9601 aaggtacaat atctataatt tgcaaatcaa gatcgtatct atcgaccatg aatttttgat
     9661 aatttacata tttgttgact ccctctaagg attcttcagc tgtttgaaat gaaattatcg
     9721 aatagtctga agctgccata gcattatcgg aaaattcgct aatggttggt ggtacatcaa
     9781 taaatatttc ttgataattg ttctttattg gaagaagtaa ttttttcaag acagtaactt
     9841 ggtctaattc attttcatgt ttattagcaa aagcggagaa tgatcgaaaa ctagtgtcac
     9901 atgccagaag gtcaagattt tcatttacct taataatact acttgctaat tctccatttg
     9961 cgacaccatt tgttatagtg gttttgatat cggttatacc gaaagttctt gctatgactt
    10021 gtgttaagtt cgcttgaggg tctaaatcaa taataagcgt ttttctattt cttatcattg
    10081 cattgtcata agcaagcatg ctgactatct tagttttacc tactcctcct ttaaaattgc
    10141 caactgtaaa tgtagtccct tgaaaagtat ccatgtaaat cctcccaatc tttaattgat
    10201 aattaaagta taacttatat atcggaaatg gtcaacatta aaagtataac ttaaataata
    10261 atatctaaaa acgtttggat atagcggttt ttaataataa aagtataagt tataccatat
    10321 atatttatgg tataacttat acttttatta ttaaaggtat aacttatacc ttaaatattg
    10381 cttttcgaaa atagctattc tagtctatct gtttctttac ttttcgctgc tgatttgaat
    10441 tgaattgatc atattcattt tctagtatta ataacttttc gtatacatcg gtcatattca
    10501 tactagaagc aagacttaga tcatatacaa atgaagcagg taatgatttt attctacgtt
    10561 gtcgggttat ccagcttgca accgttcctt gtgggtaggc atataactca caaaattgtt
    10621 ctacagtgat tcctaaattc ttgataatgt aaacattgat cgggtgcgag taaacaaatg
    10681 tattgataga catggtgcgt aagcctactt tcttttatta ttgcgtttgc aataatagtt
    10741 taacacacgc attattctag tatctactcc tatttgtaaa aacaaaaaag gattcataga
    10801 gataagttgg ccataacccc aaatctcttc tgatagctga tcaaaggtta tatactttag
    10861 ctcatattgc tggataatgt tcattctaat cgactccatt tattatcctg gtaaaattcg
    10921 atatgacgta tttcgtcgta gtgaattttg tcttttccaa cgtagatccc cagctcatca
    10981 ctacctaaga tcttgccaac aatatcttct ggatagtatc cttcagcgtt tcgttcttct
    11041 ttttgaatag atactgatct attttttatg actgcagcgt tcaatacttc cgcaatctct
    11101 tcagtcgtca tttgttcttt tgctaaattt tgactggttc gttgttcatt gtcggcatca
    11161 atttcttcag tatgatcaga taaataaaac cctgcccact ttaaacgtcc acgatcattg
    11221 taatcaatca tttttaattc ccccatttcc gcccgcatga ccaccaacaa gtgtgcttct
    11281 agtaatgctt ctagcgcttt ctaacattga agaagcatgt acaatcgacg taaaaccata
    11341 tttttgccga atcttgtcaa taattttatc taaattttct tcgt
//
LOCUS       DAFBQW010000081        11329 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFBQW010000081 DAFBQW010000000
VERSION     DAFBQW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.084"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(51..560)
                     /locus_tag="LK889_002500"
     CDS             complement(51..560)
                     /locus_tag="LK889_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096700.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDVSPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(550..1275)
                     /locus_tag="LK889_002501"
     CDS             complement(550..1275)
                     /locus_tag="LK889_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096701.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(1498..3618)
                     /locus_tag="LK889_002502"
     CDS             complement(1498..3618)
                     /locus_tag="LK889_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5096702.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3848..5026
                     /locus_tag="LK889_002503"
     CDS             3848..5026
                     /locus_tag="LK889_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBK5096703.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5042..5800
                     /gene="aroD"
                     /locus_tag="LK889_002504"
     CDS             5042..5800
                     /gene="aroD"
                     /locus_tag="LK889_002504"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK5096704.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5823..6308
                     /gene="ybaK"
                     /locus_tag="LK889_002505"
     CDS             5823..6308
                     /gene="ybaK"
                     /locus_tag="LK889_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBK5096705.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6379..7632)
                     /locus_tag="LK889_002506"
     CDS             complement(6379..7632)
                     /locus_tag="LK889_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5096706.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7749..9098
                     /gene="gdhA"
                     /locus_tag="LK889_002507"
     CDS             7749..9098
                     /gene="gdhA"
                     /locus_tag="LK889_002507"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK5096707.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9210..10556
                     /locus_tag="LK889_002508"
     CDS             9210..10556
                     /locus_tag="LK889_002508"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK5096708.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10864..11250)
                     /locus_tag="LK889_002509"
     CDS             complement(10864..11250)
                     /locus_tag="LK889_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5096709.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3683 a         2032 c         2299 g         3315 t
ORIGIN      
        1 atcatgaatc gatcttctat accgcttgtt tttactataa aatgattgat ttagcccgcg
       61 ggctctaaac gatcaatata aggactttga gcaatagtag catcatgagt aaagagtaag
      121 ataccttgat tttcttcatg agctaaataa gaaagcaagt ggagtagctc ttggatctcc
      181 aatacactaa gttcatcaaa tgtatcttca ataacaatat tgtttcgctt ggaaagcaag
      241 gcatgaagta attgcagctt tactttttca aaagacgtca actcagctgc cgcacaatta
      301 agcaaggaag gagagacatc aagtttttct tgataggcca aacgatcttg tttgatttct
      361 ttttccggta catccaacag gacatggtct ttaagattca gataaggaaa catcggccaa
      421 tttttttgga tgatcgcaaa attatccagt tttgcaacaa gaacctcttt tttcaagtgc
      481 tccaatattt tatgctgttt ttcgagagga cacaaaataa cggtcaagtg ttcagcatga
      541 atcaaatatt caggtttcat tacgtccagg cctcctactt tgcaaatgat ggataaaaaa
      601 agtgaaaggg aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc
      661 aaatccctta agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt
      721 ctgttgatcc atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg
      781 cagttcagta gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga
      841 ctggaagctt acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag
      901 ctgccagaaa ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat
      961 caattcctgc ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa
     1021 tagaaaaaat aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg
     1081 aagaagctct gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc
     1141 taaagccgat tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct
     1201 tgaaaataaa caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata
     1261 gcggattatt ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag
     1321 tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag
     1381 aggttgtgaa gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta
     1441 ttcgaaaaga ggagcagcga caataatttt gtcgctgctc cttttaaatt tattttatca
     1501 ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt tataaagatc
     1561 aacagttgat tgtccgttgt gttcactaaa gacactagtt gacttgtcac caagagattt
     1621 ttcagcttct tttaatttct ctaagccatt cttgtagtca taatcactgg tatcaattgg
     1681 gtctaatcca ctattcgtat agaaccgaag aagatcacca ttattgattt ggtcagaaac
     1741 agctaactgt ttatcaactt tttctttcag tgaatcaatt tcattctgta cttcctcagt
     1801 aggttcagta atagcttcac cagtttcgtt actatagatc gtaccaccgt agtaagtata
     1861 ttttggtgta acaaaatcac catcacggaa agaaacaagc tgttcatgat ctttcgagaa
     1921 tagatcttgt cctaactgaa tataattttt tgtatctaca cctagtaagt gaagtaatgt
     1981 aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg
     2041 gatatggatc atataaggga cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga
     2101 acttgttttt ccaactaatt cagctagatt tttatttctt gaattcgaga caccgtaatg
     2161 gtcaccgtaa aggacgatga ctgaattatc atacaagccg ctttctttca aataattgaa
     2221 gaattcttgt acggcggtat ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc
     2281 atcatttgta ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg aataaggata
     2341 atggttagat acagcaatga attttgaata aaatggttgt tgcagacgtt ccaagtattg
     2401 tacggattgt tggaaaaatg gtttatcatg caacccgtat tggaacgaat tgtcatcatt
     2461 aacatcataa taagaagaat caaagaaata atcatatcct aaattcttgt atgtctcgtt
     2521 tcgattccag aatgtaccag cgttcccatg gaataccgca ctaatgtaac cttgtgtttg
     2581 acttaaaata tcaggtgcag cttggaaagt attttttccg cctaattgtg tgaacaagga
     2641 gccttgattc aagccaaaca atgaattttc catcagcgtt tccgcatcgc ttgtttttcc
     2701 tgcttttact tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact
     2761 attgatgaaa ggtgtaactt catgctggag accattctca tctgtcaatt tataatcgat
     2821 caggaattgc tggaagcttt ctaaatggat ataaatcaca ttttttcctc tagcgagccc
     2881 gaacaaatcg tcatttggtg ctgcataatg ttcttttaca taattttcta cttctttcat
     2941 atcattggga cttgcttctg cccgcacttg gtttgtctgg taagtttgaa caccgtcata
     3001 aaccatgaaa gcattcaagc caagatattt gaccaaataa tcacgagaga atgttcgagt
     3061 cagcaattct ggacgatcag cttccgccat aaaaagattt cctgagaaaa tcataactgc
     3121 taatgtagag atcgcaaaag ccattcttgc tcggatcggg cgagggtcca acttgatttt
     3181 ttttgcaatc agcagaacag tcaagataat aaaatcgatc caatagatca catcatatgg
     3241 gcgaaacagt cgaatggcac tttctcccag accactcgct actttcccag cacctaaaat
     3301 cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa gtgacattcg aaaaaacaag
     3361 caatgtcaaa agaaaataaa tgaccatcat cgtgatataa gcaactctag ggcgtctgat
     3421 ataaagagca attccaagta ataacaatgt agttgcaata gggttgataa acaagataaa
     3481 gtattgaatg ccactttcga ttccgagatg gaaatcaact aaataggcaa aaatcgtttt
     3541 caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg
     3601 tgtttttata catttcaaga taatcttcct ttctgcactt cttaatcaca gaagtgtcct
     3661 gaaaccatgt tacacagctc acttattctc atttatttta ctagtatgac aatatagtgt
     3721 caattgaaac cagaagttta tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa
     3781 tgagcagtaa gacaagtcct atgttttttg ttatacttgg gaagagaaag ctaaagcagg
     3841 tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc
     3901 actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt
     3961 agttgattcg caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga ataagggaat
     4021 cggttgggta ttgagccagc agaacatccc atttgatcaa tctttttttg aagaattttt
     4081 tgtgaagtca aaagaaaaaa gaactttctt tttttcagat gattctacta ccgcttttcg
     4141 tatattcaat ggagaaggag acgggatcgg cggtctgatt gttgattatt atgcaggtta
     4201 tgctgttttt tcttggtata acgaaacgtt atatgcacat cgtgaattat tgattcaagc
     4261 ttttcgtgaa atctacccgg aaatcatagg aggctatgag aaaattcgat ttgaatcagt
     4321 aaatctgcct gagagccagt tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa
     4381 agaaaacgga gtaaaatttg ccgtttattt aaatgaagga ttaatgactg ggatcttttt
     4441 agaccaaaaa gaagtacggg gatcattagc agaaggtttc gcttctggga aaacagtttt
     4501 gaacatgttt agttataccg gcgccttttc tgttgctgcc gcaatgggag gggcaatcga
     4561 aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa acacaggaac agtttgaagt
     4621 gaatggtttg gatgtcgatc agcaaaaaat cattgttatg gacgtattcg agtatttcaa
     4681 atacgccaaa agaaaacaat ttttatatga catgattgta ttggatccac caagttttgc
     4741 cagaaataag aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg tcaaggatag
     4801 tctggatatt ttagcagaag acgggatact tattgcgtct acaaatgctg cgaatgtctc
     4861 gatagaacaa ttccaaacaa tgattgaaga ggaattccat gccaaaaatg tttcttttga
     4921 acaacttgct ttatatcgtc tgccaaaaga tttccagacg acggatacct ttttagaagg
     4981 caattatctg aaagtgtttg tttatcaaat taaaaataac cattagaagg agcgatgcag
     5041 aatggttcgg gtaaagggat tgaatatagg aagcggtaga ccaaagattt gtgcgcctct
     5101 tatcggggcc gtccgagagg aagtattacg tgaagcagtt cttgcacgtg aagcaggggc
     5161 cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc
     5221 agaaacattg gttcttatcc atgagatgct tgatggactg ccgttattat ttacgtttcg
     5281 taccttagct gaaggcggag aacaagacat atcgatccgt cagtatcgcg acctttatga
     5341 acatgtgata cacacaggat tagtagatat ggtcgatatc gagttgttca aagtagaaag
     5401 tctaggaaaa agactgatag aggaaatcaa gtctttaaac attcctttaa ttatcagtag
     5461 tcatgatttt aaagaaacac cagcagaccc tgtcttgctt taccgattga atgttatgga
     5521 acattttgga gccgatatcg gaaaactggc agtaactccc aataatgaac gagatgtttt
     5581 gcggttgatg gagttgacta gacgggcaaa tgcttttgtc tccatgccta ttattacgat
     5641 gtccatggga aatcttggta aaatttctcg tctggttggc ggaacgacgg gttccgttat
     5701 gacatttggt tccttaagca gcgattctac ttctgctcca ggacaattac cggtcaaagc
     5761 attgaagcaa attattcaat tattaaatga accgttgtag aacaagtttg aggtgatatt
     5821 ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga
     5881 ttccctatac agagcacgaa tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg
     5941 ctgaacagct tccagaaagc caatctcgta tatttaaaac actggttgct gtgggcaatg
     6001 tgacggggcc tcttgttgct gtgataccgg gagaagccga attgaatcta aaaaaattag
     6061 caaaagtttc agggaataaa aaagtagaaa tgctccattt aaaagatttg gaagcaacga
     6121 ccggatacat acgtggtggc tgctcgccta taggtatgaa aaaactattt cctacttatt
     6181 tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga agagggctac
     6241 agatggagtt agcacctcaa gatatctgtg cattaacgag cggacaattt gcagatataa
     6301 aacaataaat catacgtata aaagaaagaa ggaggccggg atgttgtttg tccagtctcc
     6361 ttctttcttt tttatacatt atgctctttg cccggcattc attcctgcta catgccctgt
     6421 agcgaaagcc gctgtgatat taaagccgcc agtataacca ttgacatcca gtaattctcc
     6481 cgcaaaatac agaccatcaa tgattttact ctccaatgtt ttaggataaa tttcctttag
     6541 agaaacccct ccgccagtta caaatgattt ctcaatagga aaagttttgt tgattgatag
     6601 cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc
     6661 tgtcagatga tccatttcaa gccgctcaag aaagaaaact aacaatcgct caggcaatat
     6721 accatgccaa gcattgacca agttcttttt ggttgcttta cttttttctg tcaatatatg
     6781 aataagctct tgtttggttt gtgtagggaa acaatccaga gaaagtgtca ctggttctcc
     6841 cgttttctct aattctttat tgacaaaact tgaacaacgc agtgcagccg gaccagaaat
     6901 accgaaatgg gtgaaaagta aatccatcgt atgttcagtc aatacacgac cttttttatt
     6961 taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga agtgttttct cctgaatata
     7021 cggttcatcc gatatcaagg gggactctgt cgcataaaga ggcgtcaccg tgtgacccac
     7081 tcgcttcacg atcttgtacc catctccagt agaaccagta gatggataag tccgtccacc
     7141 agttgttaag ataatggcag gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat
     7201 tttatgttct ttgtggatca atttctctac tcgagtagag aatagaagcg tcacatctag
     7261 ttctttcaga cgattgagca atgcttcgat aatcgttttt gatttatttg ttacaggaaa
     7321 catccgtcca tgatcctctt cttttaaatg tacgccttgt gattcaaaga agttcatgat
     7381 atcgaaatta ttccattgcg caaacgtact atataaaaac tttccatttc caggaatatg
     7441 tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg caccgaccac cacctgtcat
     7501 cagtagtttt tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc
     7561 agctgcactg atcgctgcca tcatcccgct tgttccagcg ccgactacga tcacatcata
     7621 cagtttttcc attccctcac ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa
     7681 aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc
     7741 gtggaacgat gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa
     7801 aggatcaagg gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt
     7861 cttttttgga cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac
     7921 cagaaagagt tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa
     7981 atagagggta ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct
     8041 tccatcctag tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga
     8101 atagtttaac cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag
     8161 gaaaatctga taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac
     8221 atatcggccc atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg
     8281 gctatttgtt cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta
     8341 aacctttgga tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt
     8401 attatgtcaa acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg
     8461 tttccggaag cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga
     8521 aagttgttac atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga
     8581 aactattgaa agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac
     8641 gtccttctgc tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata
     8701 tcgctttgcc atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag
     8761 aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt
     8821 ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag
     8881 tagcagtatc tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc
     8941 aagtggatga gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag
     9001 cagccgagta cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa
     9061 aagttgccaa agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat
     9121 attgtcagca ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct
     9181 gtagagtaaa cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag
     9241 tagcaccgtt tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc
     9301 acaacgaatt acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac
     9361 caacgaacta tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat
     9421 ctgattctga agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag
     9481 cgatcgactt cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac
     9541 cacaaatctt ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag
     9601 taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac
     9661 cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag
     9721 caaacaaacg tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag
     9781 atgcagaagg ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat
     9841 taactgctgt tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc
     9901 aaggtgccgc tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt
     9961 accaatacgc agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga
    10021 tcaactatga accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat
    10081 ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc
    10141 actctttagg acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg
    10201 ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag
    10261 gctacttgca aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc
    10321 ttgctcatac agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt
    10381 atacattggg ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga
    10441 atggaatcaa cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac
    10501 ttctaggcaa accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata
    10561 agggtttttc gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa
    10621 ctggtaccca tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga
    10681 caagacgttt gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag
    10741 tttgttttag gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt
    10801 tttctagaat cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt
    10861 agcttaagat aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac
    10921 ttttttctct atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta
    10981 agcagaacca ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc
    11041 accgttttca gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt
    11101 atctgctttg atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt
    11161 acctacataa gtagactgat agtagtccca acctttccat cctataaaag caatcaatat
    11221 gataacgatc aaccctaaaa tttttttcat gaaagatctc cttttttatt tttattatac
    11281 ccgtggtgct agttaatttt aatatcctaa agaatatctc aaagttcct
//
LOCUS       DAFBQW010000082        11270 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFBQW010000082 DAFBQW010000000
VERSION     DAFBQW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.085"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LK889_002510"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LK889_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK5096710.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LK889_002511"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LK889_002511"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK5096711.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LK889_002512"
     CDS             complement(2169..2906)
                     /locus_tag="LK889_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK5096712.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LK889_002513"
     CDS             complement(2997..3524)
                     /locus_tag="LK889_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096713.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LK889_002514"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LK889_002514"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK5096714.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LK889_002515"
     CDS             complement(4401..4886)
                     /locus_tag="LK889_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK5096715.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LK889_002516"
     CDS             complement(5039..5236)
                     /locus_tag="LK889_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096716.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LK889_002517"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LK889_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBK5096717.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LK889_002518"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LK889_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBK5096718.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LK889_002519"
     CDS             9211..9423
                     /locus_tag="LK889_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBK5096719.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LK889_002520"
     CDS             9425..11104
                     /locus_tag="LK889_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK5096720.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3272 a         2434 c         1974 g         3590 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa
//
LOCUS       DAFBQW010000083        11140 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFBQW010000083 DAFBQW010000000
VERSION     DAFBQW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.086"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            233..664
                     /locus_tag="LK889_002521"
                     /pseudo
     CDS             233..664
                     /locus_tag="LK889_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(721..1401)
                     /locus_tag="LK889_002522"
                     /pseudo
     CDS             complement(721..1401)
                     /locus_tag="LK889_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1475..2125)
                     /locus_tag="LK889_002523"
                     /pseudo
     CDS             complement(1475..2125)
                     /locus_tag="LK889_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            2450..2866
                     /locus_tag="LK889_002524"
     CDS             2450..2866
                     /locus_tag="LK889_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK5096721.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            2863..3333
                     /locus_tag="LK889_002525"
     CDS             2863..3333
                     /locus_tag="LK889_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5096722.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3347..4120
                     /locus_tag="LK889_002526"
     CDS             3347..4120
                     /locus_tag="LK889_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5096723.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4134..4946
                     /locus_tag="LK889_002527"
     CDS             4134..4946
                     /locus_tag="LK889_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5096724.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5000..5965
                     /locus_tag="LK889_002528"
     CDS             5000..5965
                     /locus_tag="LK889_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5096725.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6081..8804
                     /locus_tag="LK889_002529"
     CDS             6081..8804
                     /locus_tag="LK889_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5096726.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANCMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8817..9449
                     /locus_tag="LK889_002530"
     CDS             8817..9449
                     /locus_tag="LK889_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5096727.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10348..>11140
                     /locus_tag="LK889_002531"
     CDS             10348..>11140
                     /locus_tag="LK889_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096728.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3728 a         1720 c         2129 g         3563 t
ORIGIN      
        1 ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa agaggaagtc
       61 caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata tacatccctg
      121 ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga atgggataaa
      181 atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga tagtgaacat
      241 acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta ctttcaaaag
      301 attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat acaagtttaa
      361 ttttgtttaa tcttcagtat ctactgaaag tatctatttt tcaattcttt ctgagagtag
      421 gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata tatatacgtt
      481 ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg aatatctcaa
      541 ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac catatttgaa
      601 tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta atcaaggaaa
      661 tatgaaggtt ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc
      721 ttaggctgtt attcccatta ataccttgat ttcagtagac accgaaaagc cgaagagcgt
      781 tccatttctt cggttctttt tatatattcc tcgaatggtc tccatgccct taatcgtgga
      841 agaggctgta cggagacttt gataaaattt atttcgtcgt ttaataggtc gatggtcttg
      901 ttctattaaa ttgttaagat acttcacagt tcggtgctct gtcttagtat ataaacccac
      961 actctgtaac tttctaaagg cggagccaag agaaggtgct ttatcggtca caattgcttt
     1021 cggctcacca aactatttat ggagtcgttt taagaaagca taggctgctt gcgtatcccg
     1081 tttctttcgt aaccagatat ctaaggttaa gccgtccgct tcaattgcac gataaagata
     1141 atgccaacgt cccttaattt tgatataggt ttcgtccatt ttccatgaat agaaggattg
     1201 tctatttttc ttcttccaaa gataatagag gactttgctg tactcttgta cccaacgata
     1261 aatcgtagta tgacaaacat ttattccacg atcatataac aattcctgaa cttcacgata
     1321 gcttagattg taacgcaggt agtaaccaac agcgacaata atgacgtctt ttttgaattg
     1381 tttgccttta aaatgattca ttactctgtc ctctctgtct tttttctcaa ttttacacta
     1441 aaatagattt tttggaaaac tttgcaacag aaccgccaat catatggaag ccaccgatta
     1501 ggtattttat gggtttagtc ggaaatgttt caagagcaat ttccatcata tcgataatgc
     1561 catgatgact acagccagta aagacaatca gaccatcttc ttgctcaatt acgaccaata
     1621 attcatctga aaaatcatca taaacaaatt tatcatccat ctttttgtat aaattcttgg
     1681 tggtcgagtt tgctttgcct agggggatga tatggaagct cggagcaagt tctgttttcg
     1741 tttttattgt atgtaaattc tcatgcattt taaggatttc atttttagga ataccaacat
     1801 acagaaacgt ggttaaaatc tttaaataat ggggtaaagt tacaccttca gctgtataaa
     1861 taggggcgtt tcctgatttt aaatatctta acccgccggt gtggtcagaa tgaccgtgtg
     1921 aaataatcac tgcctccaga tcattcaaag tttcccccat tttagtcgca ttatagacta
     1981 attttttatt agcgccagca tcgaagagat atttcttccc tgagtagtta attaagagag
     2041 ataacccatg tccactttta aagttcaaat ttattcttga gttttcaaat aaaaccttga
     2101 tttccactat aacatctcct ttcacatgaa cgtgtattca tatattgtag cacattaagt
     2161 tctatttttt gttgtaatta ccgaaaaaaa tattaatgga cgctttttac gctttatttc
     2221 gtatgtggaa gaaaatttct taggatgtgg cgggggattg gggcaaagct aaattccaat
     2281 tacagtgatc atttaattag acacctttaa atacccacac tttcatcaac actataattt
     2341 agggaaagaa aagtgttggc atgatataga aaaaccttta attaacagcg tttcctaaaa
     2401 ttggcacgca tcttgcttat agaaaagcgt aaggatagga ggaatgaaaa tggcaactat
     2461 aattcttgcc agccatgggg aatttgctca aggattaaag caaacagcga caatgattat
     2521 tggagatagt gtaccaattc atgccttgtc agcatttaga gatgaagatg aatctatttt
     2581 ggtacaaata aaaaaacttt tagccacaat agatatagaa gaaacctaca ttttaactga
     2641 tattctaggt ggtagtgtga ataatgatat gctcactatt attaaagagt atgagggatt
     2701 gcaattaatt actggaatga acttaccttt agttatttct attgcaacct ataatggaaa
     2761 aattggggaa agtgagctag aaaagcttat taaagagagt caacaatcag tgattgattg
     2821 tagaaaatta ttagtagaac aacttactgg aggagatgac ctatgattaa gttagtacgt
     2881 attgaccatc gtttgttgca cggacaggta gtattttctt ggagtaaatc attaaatatc
     2941 aatcgtatta ttattgccaa taaagaggca gcgaatgatg aattcaaaaa aatgtctttg
     3001 aatttatcta aaccgactgg cattaaatta acaatttttt cagttgaaca gttactagaa
     3061 aaaatgccta aaatcgaaac acttaacgaa aatatcatgc ttatttttgg taatacaaaa
     3121 gaaattcgtc aattttgtga agcctatcct aaagtacaag aaatcaacta tggagggata
     3181 atcaaaaaag aaggttccaa gcaatttagt aatgccattt tcttgaatga atctgagcaa
     3241 gaagatgcca aaaaattaaa agctcttggc attaaacaat ttattcaaca ggtaccaact
     3301 tctaaaaaag aagatttgaa tacaatgtta taaaggagga atgaaaatgc tcgttcagtc
     3361 aattggttta gggttaatcg gtgttttttg tattttagac tcaaggcttt taggaagaat
     3421 gaattttgaa cgaccgttga ttgtcagtac gcttgttggt ttggtacttg gagacatgga
     3481 aaaaggatta attgttggag cctctttgga attgatgtcc ttaggattag taaatatcgg
     3541 agctgctgca ccacctgata tgaatatggc atcaattatt gctacagcct ttgccatttt
     3601 atcaaatgcg aatgcagaag cagcattaac gattgccatt ccaatttcag tgttaggtca
     3661 aatactaggt atcttaatgc ggacgatttt gtcaaatttg actcacgttg cggatcgctt
     3721 tatcgaacaa gggaaattta gccaagcaag gagaattcat atactttatg gctcgggatt
     3781 atatgctctc atgtatttta ttcctatttt tctagcaatt tattttggaa cgaatcttgt
     3841 ttctcagatt gttgatgcga ttcctgagtg gctaacaaat ggcttaacgg ttgcaagtaa
     3901 aattttacca gcatatgggt ttgcgttgtt attacaaacg atgttaacga aaaaaatggt
     3961 gccattttta ttacttggat ttttaattac tgcttattct ggattgggaa tcattggtat
     4021 tgcagccttc gcatgtattg ttgcgtttgt catggttcaa gttcaaggta aaaactcaaa
     4081 tggtaaccaa aatgagccag atccattaga tgacttataa ggaggtagat taagtgaaag
     4141 agaaagatca aaatgcatgg aaatatcggc agttcttatg gcgttcatgg gcaattcaag
     4201 cctcatggaa ctatgaacga caaatgaaca tgggttttat gtacggaatt gcaccaattc
     4261 tagataacat ttacaagaat ccagaagatg aagcaatgaa gaaagaagct tacgagcggc
     4321 atatgatgta ctacaactgt acccctcaaa caagtgcatt tgtgttaggg ttaactgctt
     4381 ctatggagga gcaatactac gaagaccaag aaaactttaa tccggaatca atcagtgcag
     4441 tgaagacttc tctgatggga ccgctatctg gtgttggtga ttctttcttt caaggaacga
     4501 ttcgggtatt agcctttgga ctaggaatta atttagccca acaaggtagt attttaggtc
     4561 caattctagc aatgttaatt tcctttgtac cttccttttt agttacttac tatggtggga
     4621 aaattggcta taacatgggg aataaatact tgtctaaact gtatcaagaa ggtttgatgg
     4681 ataaagtaat gtatgtttgc tccattgtgg ggttgatggt cattggttcg atgattgcca
     4741 gcatgatcgg gattacaaca ccaattcaaa tgggtaaagc ttttgtctta caagatgtat
     4801 tagatagtat tatgccacaa tttttaccat taggattaac cttcttcatg tactggttat
     4861 tgcagaaaaa ggtcaaaaca ggatggatgt tggcaatctg tttagtcgga ggaattattt
     4921 ttagtttgtt aggtatctta caataaggga ataaccaata aatttggttg aataatataa
     4981 ataaggagga attcaattga tgaattcaaa agaaattgtt aaagaaatcg tagcgaaaaa
     5041 agaggtaaaa tcaatcgtat ttgttggctg tggcgcttca aaagcggacc tttatcctgc
     5101 aaagtatttt ctagaccaaa atgcgaagca gttacggatt agtcattaca cagccagcga
     5161 atttaactat gcaacaccag catctgtaga tgagtcaact gtcgttattt cagcttcttt
     5221 aggaggtacg actccagaaa cagttgaagc taacgcagta gctaaagaaa aaggtgctac
     5281 agttattagt ctaacacgaa gcattgactc accgttaaca aaagatgcag attatgtaat
     5341 ttatcatcga tttgcagaga actatgctgc taaattagaa aaaacaggct actgtttaga
     5401 attggcagta gaacttttac atcaagtaga aggttatgac cattatgaag caatgcaaga
     5461 aggatttgct actatctatg atcttgccca aaaaagtgca gaatcagctc gtaaagatgc
     5521 gaagaaattt gctgaacgct ttaaagatga aaaagttatt tatgtaatga gtagtggtgc
     5581 tacacatgaa gtagcatact caacatcaat ttgcttgatg atggaaatgc aatggattaa
     5641 ttctggtacc ttccattcag gagaattttt ccacggacca ttcgaaattg tggataaaga
     5701 cgtaccgttt atattattaa tgaatgatgg aaaaactaga aatatagatg ctcgagcatt
     5761 aactttcctt gaacgctttg atacgaaaac agaagttgta gatgccttgg attatggctt
     5821 gtcttcacat attgctaaag aagtcttaga ctactttaac ccattcgtga ttactgcagt
     5881 atttagagtt tatgcagaag aattgtcaga agcaagagaa catccattaa ctaagagacg
     5941 ttatatgtgg aaactaactt attaaaaaat agatcaaact aaataaggtg gcttgagaaa
     6001 ttaagccacc ttgttttctt caatgtgatt ttatacatat ggtaaaataa ctagtacata
     6061 ttgtgaaagc aggtggcaag atgcagcgat taaatgacta tctttatcaa ctaacattgc
     6121 ttttgaaatc tacgccttca gataattatt cagctcgcag tatttcgcaa gctttacagt
     6181 tgaatagaag tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa
     6241 aagtaaaaag ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca
     6301 cactgaataa ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg
     6361 cattagatca agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa
     6421 cggcagtctt atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag
     6481 ggaaaacctt tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca
     6541 catcggcacc tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat
     6601 cctctgcttt atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg
     6661 gtttgctaga aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg
     6721 aagaagggca agagaaatta tttacattta tggatacggg tgaattctct cctatggggg
     6781 ataatagtat tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat
     6841 atacgacttt cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat
     6901 ttcaacaacg tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga
     6961 gtaaaatttt agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata
     7021 gtgattttga aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg
     7081 catatatcca ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag
     7141 agtacagtta taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa
     7201 tctactatcc agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc
     7261 aattttttga ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta
     7321 aactattttt agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc
     7381 aagaggatta cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa
     7441 gtattgattt tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt
     7501 tggccaacta tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa
     7561 agaaaaccaa agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt
     7621 atgcaaaaaa aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg
     7681 acattttctt tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta
     7741 ttaagagtat tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca
     7801 actgtatgct gaataagaac gtttttcaag cttatgatat gcctttagat attacattag
     7861 agaaaattga agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga
     7921 ttttattggt agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa
     7981 atggaccact gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac
     8041 aagtattaaa caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt
     8101 tcaaaaagca gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt
     8161 atacagggat tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg
     8221 cagttagaga attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt
     8281 ttgagactcc ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa
     8341 ttaatcagat tccatatatt ggattagatc gcctgattaa tggggaagca attggcgaat
     8401 ttgttcatta cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa
     8461 ttttcaattt ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg
     8521 tgctatcttt tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt
     8581 ctaataatca gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac
     8641 gtaaagaaag tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa
     8701 taatggataa aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac
     8761 cagatttaga gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga
     8821 caatgggagt aatttttgat atggatgggg tactcataga cagcgagaca ttttattttg
     8881 agagaagaat gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg
     8941 attatgtggg gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat
     9001 taagagagga actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg
     9061 ctaaagattt acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac
     9121 cattggcact ggcatcttct tctccagttg ttgagataga gagtatgctc attcaatgtg
     9181 aacttactga atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac
     9241 atcccgaaat atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag
     9301 aggattcaac gattggtatt aaatcagcaa aggcagcaaa tatctatacg gtggcactaa
     9361 aacaaccaat gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac
     9421 tggaacaaat tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt
     9481 gctagctatt ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg
     9541 gtaacacctt ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt
     9601 attttgttcg caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc
     9661 aacattctat ctatttcaga acgtaacctt ccccagttca ttgggattca ttagcataca
     9721 accaacattt caaaaagaaa ttgcactaca ccattattag ctattttgac taaccccaaa
     9781 aaacttgcta taattcaggt gtaggtaaaa ggtgctggta tcacctttta cgaaactgct
     9841 tcggcagtgg taatgagtgt ggcttaaagg actagtcctt taagcttttt actttgttcg
     9901 caatctctaa gatcaagtta atgatcgata agattgctag aatattttgc aacattctat
     9961 ttcagaacgt aacctttccc attccactgt gattcattgg caaccctcct cacaaccaac
    10021 gtttcaaaag aaattgtaaa gttgttgggg taccacgacc gaacaatact acacctgaat
    10081 gatagtaagt tgtctcatta agtatagcat atcctctttt tgggtaaata gaaatcggtt
    10141 acatttttta tccaagacca agttttttca tttggtctta tttgctatac ccaaacaatt
    10201 ccgcagtata gtcgacggta ttgattttat caggtgagta atagaaactt gttttccttc
    10261 cccaaaacat tacctataaa ggagaaaaaa tggtatcgta attatatagg tataggatga
    10321 aatttttttc taagaggagg gttgttgatg aaaaatttac agttaaaatt tattgttgat
    10381 ccatcgattg tacgctggtt tcggattatc aatcaatttg agcgcaatca acttgccaca
    10441 aaaggagaat tagcaaagag taatcatgta tctgaacgaa ccattcaaac agatgtcaat
    10501 aaaatgaagg actacttttc tgaaagtgtt caatttgttt caacgaaaag tgggtattct
    10561 tttcagaagg aaagaccggt tctttttctc aagcagaagg agcaactact ggagaacgaa
    10621 attttatttg agttgttagg gaatgtcttt tatggcgaat tggaagatat gacggagcta
    10681 attgatcgtt ttcatttatc tgaagcgact tttcgtcggt atttgaagca gattcaacca
    10741 gttttggaag aatacggctt agaattgagt ctttatcctc ttgatttaaa aggagaagag
    10801 gcaaatattc gcaagttttt taaagacttc tattatgaag gggagatgac gccccatact
    10861 cttgttccac caagggatct tcatgaactt gtgcaagcta cattctatga taaatttgag
    10921 tatttctcga ttggaacagg gacttctcct gctgaatttt attatagttt gtatattgcg
    10981 attgagcgtg tgagacaagg aaaaacgata gccattcctc aaggtctgat tgagcgagta
    11041 attgctagtg agaattttcg cttaatgtat tccttgaaag aagcgattca aacaaattat
    11101 caagttgctt tatcagaaga ggagttttgt tggttgtatt
//
LOCUS       DAFBQW010000084        10771 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFBQW010000084 DAFBQW010000000
VERSION     DAFBQW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.087"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..986)
                     /locus_tag="LK889_002532"
     CDS             complement(<1..986)
                     /locus_tag="LK889_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5096729.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLV"
     gene            complement(991..2904)
                     /locus_tag="LK889_002533"
     CDS             complement(991..2904)
                     /locus_tag="LK889_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK5096730.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3072..3578)
                     /locus_tag="LK889_002534"
     CDS             complement(3072..3578)
                     /locus_tag="LK889_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5096731.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3738..4178
                     /locus_tag="LK889_002535"
     CDS             3738..4178
                     /locus_tag="LK889_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK5096732.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4204..5361)
                     /locus_tag="LK889_002536"
     CDS             complement(4204..5361)
                     /locus_tag="LK889_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096733.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5364..5720)
                     /locus_tag="LK889_002537"
     CDS             complement(5364..5720)
                     /locus_tag="LK889_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096734.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6018..6992)
                     /gene="sgrA"
                     /locus_tag="LK889_002538"
     CDS             complement(6018..6992)
                     /gene="sgrA"
                     /locus_tag="LK889_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBK5096735.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7188..8027)
                     /locus_tag="LK889_002539"
     CDS             complement(7188..8027)
                     /locus_tag="LK889_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5096736.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8212..9099
                     /locus_tag="LK889_002540"
     CDS             8212..9099
                     /locus_tag="LK889_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK5096737.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(9693..10388)
                     /locus_tag="LK889_002541"
     CDS             complement(9693..10388)
                     /locus_tag="LK889_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK5096738.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(10372..10770)
                     /locus_tag="LK889_002542"
     CDS             complement(10372..10770)
                     /locus_tag="LK889_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK5096739.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3218 a         2159 c         1618 g         3776 t
ORIGIN      
        1 attactaact tttgatatgt ttcttgcgct tgtttattgc tgaaatcaaa ctgatattca
       61 tggttcaaaa cagcattttt ctccgcataa tctacttttg tgactttcac ccccgctttc
      121 tcaaatacag aagttacttc ttccatttgt ttcgtaaacg tatttgtatt tccatccgtt
      181 aagaatgtgt tttgcgtcca acgatccgca tgtctgacca aggctaaact tttgaccttc
      241 tctgattgtt caaagtcctt attatcaaag taagcccatc cacatttttc ataaagaaag
      301 ctagagattt tatcacttac ttgagtaagt gcagaaggat ccaacagacc agacaaagaa
      361 ataaacttcg taatctgctt gttctccaaa gtcggatcaa gatgatcagc ttttttaatt
      421 tcttcatttt gaagtgctaa caaatattct ccactaattt gtgcacctgc cgaatttcca
      481 ccaatcgaaa tattcgacca atctgcttga tcttttatct cttttttagc tgcttgaatg
      541 gcttcgttca attggataac tggtgtagga tgttttgctt ccggtgatcg ttcataatct
      601 atatttacga tgatttgctg cgtgtctgaa catagatatt ccaaataatc acgacaatca
      661 tttttatccc ctcctacata tgcgcctcca tgcatccaga aaagaatcgg taattttttc
      721 ttttccactt tcggtgtgta gatatccata ttattatttt tgtactttga aggataggat
      781 atttcttttt ttatatcgat ttgttcgatt ttttgctgga aatcatctgg tcgggcatat
      841 acttgttctt tttcacttgt gtcaaattgt tttctaacaa gcaagctaat cggctttggt
      901 gtgatttgat taatcaaaaa tacagtgact aaaataacta cgatgataac tggtactgca
      961 atcactattc gcttgaacca tttcattcat ttaccctctc tttcttcgcc aaatccatgt
     1021 gataatcgtg ctaacagata aaaatccacc tgaataaatg agaagcgaat gtttcttttc
     1081 tccagttttc ggcaacattc ttttttttgc atctgtcttt ttttgtactt tcactgtcat
     1141 ctgaatcggt tgcgtatcat cacttgattg atcctctcga ggttcattct ttcccttatt
     1201 gtctgcattc tctgtcggat cttgcttttt tggtggagct actgggattt ttttgtaata
     1261 gaaaataata gttggtatcg tctcattaaa aatcccttca gtcggtcctt caacatttgc
     1321 taattcgtat ccttcgaatg tcttttgctc tgtcatataa ttttctccat aagctccgta
     1381 aagctggatt tcgtcctggt ctttgagtag acttttattg ttttcatcca catactttac
     1441 gtttatctga tcatggaaca ctactttcaa aaagtccgtt acatgtttcg tgtaggcagc
     1501 atggtcaact tcctgtgcct ttccatgccc tgctccttct actaataaaa gatctttata
     1561 ccctcctgat aactcatata atttttgcgc gttagacaca ggtactacat catctgctgt
     1621 tccatggata atcaatttag gcaatgaatc tgacattcca atcgtaggta gagcagcttt
     1681 tgtatccaaa tgcatctcct gatcaaaata gtattgtttc aaaaggccca taaataaatc
     1741 ttcatgctct tgatccccaa tgatgtctaa ctgatttttt atggcatccg gtagtgcggg
     1801 ggctactgtc tggctgtacg taaaacgtag ttgttcatca attgatgcaa atccgcaatt
     1861 ttcaataatt cctttgacat tgacaggaat atctttcgct aatacagaca tcatcgtagc
     1921 tgctcccatc gaaccaccat aaagtaaaat cttttgagat ggccgctctc gaacttcttg
     1981 atttatccag taaaggacgt catctgactc atattgtcca taggtcacat aatcgcctcc
     2041 gctattacca gtcgctctgg aatcaacaaa taataaatta tacccggcct tataaaaatc
     2101 attgtattct tctgtaacga tcccgttatt ccaattccct cgaaatccgc catggatcat
     2161 gactgtttta tctgaacctt gatcgacata atatgcatat acatccatca ttttgccatc
     2221 ttgtttgttt agtacagttt gatgaagttg ttttgtattt ggatagaagc ctgtgttttc
     2281 tgtaggcgtt gtcccatcat ctgtacctcc aagcatcgac atcaacacac tgttttttgc
     2341 aaaagcgata tcgtacaata gataagtagc aatcagatgt ttgataatct ctttatctac
     2401 accataatca ttaacagcaa gctcagtgac taataactct attaattttg acttgatgtc
     2461 ttctgttggt ccattaagta tttctgtcat ctgtttaccg aagtaattca aatattcttt
     2521 accattgaaa ttttcacctt taccgatatc atcattttct gtcccattct ctttgtcact
     2581 tactttagca gaagaggatt cgtcgtttga catttttgta gctgttgctt cttctgtttg
     2641 ttggttatct gtggtagcgg tcgtttcttg atctgtgcta gtaacagatg tctcaaccgt
     2701 tgtttcagta tttgtttctg tttgtgaagt actcgatttc gtttcatttc cagcatcagt
     2761 agtttctgtg gtttctacct cccgagattc cgattcggaa gaaatgctct ctccatatac
     2821 tgtccattgc gggacgcttg tacttacagc aaataagatg accaacgctc cctttaatac
     2881 ttgttttgtt ctcaagctat tcactccttt ttatgtttct aggagaatta tatgtaaacg
     2941 caaacatttt ttatacaaaa aataaatggt ttatcccata aaaatagaac aggattattt
     3001 tctcatttta cgtaataaag cagaatggat aaaccagtat ccattctgct ttttttaaac
     3061 gaagaaacaa cttattgctc caaaaatgtc agaaatcgtt tatcccaaat tttctcgtaa
     3121 aaagcgattg tttcctttgc tttcataaaa ggattatcat aagttgtcgt tgctcctcta
     3181 cacatcataa gctggtatcc taatccatga gcaaacttca cagtcgtatc tatacaatac
     3241 tctgcttgtg ccccacataa ttcgatcgaa tgacagtcca attcagttaa taaaacactc
     3301 aaattagtct tataaaatga attcgcatgg gtttttctca caaaataact gtgctcagga
     3361 atagataatt ccgacaatag ctcccatggt tttgtttgtg gcactaattg agcatcttcg
     3421 tgttgaacga aaataactgg tttcccaatc gtttcgtatg ttttgattct tttattcact
     3481 aatgctgtca ctgtttctaa ttcgtgtatc ggcgaagaat caaaacatac ccctttttgt
     3541 aaatcgatca ctactattgc gtcggctgtg tttgacatac ttgtgccttc tttctttttt
     3601 cttgatttaa tagtatttct tatctagcta gttccattat acgtgacaac attttcttat
     3661 tttacacctc taaatggcac ttttcgacta tttcttcatg ttattatttc ataaatactt
     3721 agctattgga ggaatgtatg atgattaaat tgtatacagg ttctggctgc tcgtcttcaa
     3781 gacgcgcaaa acgttggctt atcgtccatg atataccgtt tatcgaacag aacttaattc
     3841 gtcaaccact aaccaaaaat gaatttatcc acttactaaa attgacaaac aacggcgtga
     3901 ccgaaatgct ctctactcat tctattagtt ataaaaatct tgttcacaga atagatgaac
     3961 tcagtttgag tgaactatat attgagattc gtacatcccc ttctttattg aaaaaaccga
     4021 ttatctacga ccaatctgca aaacttttga ctggatttag cgaagataaa atcaggatgt
     4081 tcctttctaa aactaaaaga aagcaagagc tcaacgaact cttgctttct ggtcatcttg
     4141 tcgattttaa agaaaataat ctttttgcaa tgtcttaatt ttggcataaa caatctgctt
     4201 ttcttaatta acgaaatcca atttttccaa tatggatcta tttttctcat tcctgatttt
     4261 tatgatttca gctttcagac gcgcccaatc attttttgta ggttgtccat tccagtaaaa
     4321 tatctgtgta ttcgtttgat catatgcctt tttgtcgata ggataatcgc taatgacaat
     4381 atcaggtaac tgatcagcgg atgcactata ttttattggg cgttcagtca tagataatat
     4441 ccttttcttt gcccataatt cctgtgtttt accatacttt gattgaatat agaatatgac
     4501 aggttgttcg tgttttataa aaatatccct gactaacata cagtattgaa aaatcaaccg
     4561 tctgttcttt tcataataag tattaagctc tggaattctg cgagctaccc cactaaaaaa
     4621 ctgttgcatg attggataca tcactggaaa tatatgctga aaatctctgt cttctgctga
     4681 ttttccaaaa gcatccacct tcttttttgt cccaaaaacc cttgaataca aatggatcat
     4741 tgccaaattg acatatagat aatttttttc tgaagttgta aaacaaatct tgaagttttt
     4801 ttcaaaattt tctacaatag acgattcaat ccagccccaa ctttcttgta ctttatctgg
     4861 agtatccaac aaatcttctt ccaaataggt gttcattaca ccaaacataa aataaaggta
     4921 agcttgctct gttggagtga tgtgattcat cgaccagaag tttgcttcaa aaaaaggctc
     4981 tagtatgtaa tgcgtaaact cttcttttgt tatcaatgga tgcacaaatt tgttcatctc
     5041 ttctgggaca tacgtaatcg agtggccttg tttcattcgt tgcaacgaaa ttcttagtag
     5101 gctgatccat ttctttctat ctatactacg cgtatatggc gcatgtttcg caaatttttc
     5161 taatagttga cgtttatttt ttatacttga taatatatca gaatgatttt cgtcgaatga
     5221 ctgccaatac atcaagtgaa aaaagtatcg gatacggtat tcttctcctt ctatcggatt
     5281 accttttttc aagctgcaag ataaatgata gtttttcaaa atagtattaa gttctcttaa
     5341 ttttctttga atcgtacgca aacttatata attttttgac taagattttc taaggattga
     5401 taagtacctt tgtaaagtga atccagtaaa atataaccaa tcgttccctc taaaagtata
     5461 gaataaactt tactcagtaa aaactgagaa gaaaactcga cttttatcat cttgttttct
     5521 gaaagaaaag ataatttata ttctggatac ctttcgcaaa acgattctgc ttgttgaatc
     5581 accgaattca tcttagccag ggtgtagttt agatctaaag cgatatcctt caccacatat
     5641 aatttctcgt ctctttcata acaatagttc aaaaattgat atacttgcct ttcattgata
     5701 tcaataaatt cttcaaacaa agtacatact cctttttatc tattatttag acttattccg
     5761 acagctacat gaataacgta caaatagatt cgttactgat ttttcagaga tactaccatg
     5821 cgcagtgtgc attgtcaatt attcttgctc ttattttcca tttgtgttcc tgttatttcc
     5881 tgtttctcaa atatgttagt aaacaaaaat agatctggca aagctacaac ttcgccaggc
     5941 aatacagcac tctcgaatga aatattcact caaaaatcta tcgttttatt caattttaga
     6001 tctgttgctt ttattcttta tttcactcga tggaagagag aacgaaaata gcttgctcct
     6061 acaatcaaaa tgagtaacac acccatccat gtaattagct tgttcaaggt tctactacca
     6121 gtttctggta aatcgctttt tctattatct gtttctttcg ctttttcttg atgatcaatt
     6181 ccacgagaaa caaaatcagg ctgttttagc acacttttta ggttgctccc tgcatttctc
     6241 ttttgttcct tagttggtat gataggtacc aaatcaagtt cattatttac ttttcctgtg
     6301 ctgctttcgc tgctcgtgct tcctgtgcta ctctcactac tcatgcttcc tgtgctactt
     6361 tcactactcg tactctccgt actgctttcg ctgctcgtgc ttcctgtgct actttcacta
     6421 ctcgtactct ccgtactgct ttcgctgctc gtgttttctg tgctactttc actgctcgta
     6481 ctctctgtac tgctttcacc gctcgtgttt tctgtactgc tttcactact tgtgctatcc
     6541 gtactgcttg gcggtccaac aactgtccaa ctgcttggtt tcaaattctc cgtgatcgtc
     6601 atctgtccat cataaaaaac aatcaaatca actttatctt tactcttgat ctcatcaatc
     6661 gatagttctg cagaaatcat tattgtatac tcaataccag cggtccattg tacgtctttt
     6721 aaataaactt ccccaataac tttttctgaa cttccattac catcccaacc atatgttaga
     6781 acagcatgta catcactagc attcacgctt ttgtttggtt tgactggaat agtaatgact
     6841 tgttgtgtca tgtcataaac aatccgatca gttttcacag taaatgaaag attctcattt
     6901 gcccgttcat ttgcagaagt actgattcca atcattgctg caattatact cagcccaaga
     6961 ataacaaata atacggtcgc tgttttcttc atttctatca ctctccatat aattaccttc
     7021 tatttgataa aggaattata taaaatgaaa atgatgagaa cacgacagtc agttgtgaaa
     7081 atctaacaaa agatgtcgtg tattcgcttg aatttgttta ctttttcgtt taatcaatga
     7141 cagacttaaa aaagcaagac cgatatcagc cttgctttct tcatctttta ttgataaaca
     7201 gcagtgtatt tcttcatccg ctttgtttta tatgcataga gtaacaaacc aacaatcaag
     7261 actaacaaga ataatgtagt tgatatagat gcttcagccc caaagtttcc cccagtaatt
     7321 attgtatttt gcgattggat cagagtggag aaaaaacctt gttcttgcgt tccgctgact
     7381 ggtataccca aaatagctcc ttgtgtccaa ttccaagtaa tatgagcacc aatagccgta
     7441 aataaacttc ctgtactgta gataagtgcg ctgaaaaagc aacctgctaa aaagatattg
     7501 aaaactgcca gaaatgtcat gttaggattc aatccgtgca aaagagcaaa taatgcagca
     7561 gatccaatca ttgctattaa cggaccaaat ctaagcatct gatgctgaaa aaagccacgg
     7621 aataaaagct cttctgatgc ccccactgca ataaataaaa taaaactcgt tatcagcggc
     7681 acaatgtttg cgtttcctga atctaaccga tacgcccccg tcaaatataa tggactagta
     7741 ataagcagaa acactccaat tccaacaacg agacctcccg ctaaataccc ccagtctttt
     7801 cctcgaacag aaacaccata atcttctatt ctttttcctt cgaccttact agcaagaaat
     7861 aaagctagta aaaaaacagc taaatgtccg accgtaccgg caattgctcc aatccattca
     7921 ctatttccaa taaacatatc cgcaagtgca ttattgacaa gaattcctaa aacaaaacca
     7981 actaagacta ttaaaacaga tccaatcaac cgaataatcc tgatcatctt tcatccctcc
     8041 tttatattaa gtttaacaac ccttttcttt cttgtctcgt gaaaactatg taaatgttct
     8101 ccatcataga gagaatagat ttttacgaac taagaatatc tacagcaacc ttacgaaaat
     8161 gaaagactgt ctccctaatt cttctgttag actgttttta ataaggaaag gatgggaaat
     8221 tttttgaaaa aacaactact aattatgtgc ctttcagcat gtgcaatttt actattctca
     8281 ggttgtagca agtccaatga aacgattgca acgtttaaag gtggaacact tacagttcag
     8341 gatttttatg ataacccaaa agttaaatca aataatcaaa cactattaaa aaaaatgatt
     8401 gtcttcaaag catttgatga tgtatacgga aaagagattt cttcaaagga agtaacaaaa
     8461 gaatataatg aacaaatcaa aaaactcggt ccaaactaca aggaacaatt aaaagcagtt
     8521 ggccaaacag aagagacata taagaaactc tttaaacaga tgctggcttt ccaatacggt
     8581 ttgaaagcca atgtaaaatt aacagataaa gatctagaca ctgcttggaa agaattctat
     8641 ccagaagtta gtacacagat cattctattt tcgactaaag aagaagcaga taaagcaaaa
     8701 aaagaagcaa atgaaggaga aaatttctcc aagcttgttc aagcttatgg aaaaaataaa
     8761 acattaaagg aaactgatgg aaaaatgaat tttgattcaa ccaatccaga gattcctact
     8821 gaagtaaaaa aagcagcatt taaactaaaa aacggggaag tttctgatat cattcctgta
     8881 acagatccaa cgacttatca gcaaagctat tatctagtaa aaatggtaaa aaaacaggat
     8941 aaaggttcta ataaagacaa atacaaatct gagctagaaa aaattgctac cgaagcaaaa
     9001 actcttgata cggaattcat ggataaaaca atccgtaaag tgatgaaaaa agacaatgta
     9061 actattaaag atccttatgt aaaaaatata ttcaaataaa caaatttgac ccaattgggg
     9121 atgtggcaaa atttgagaag tgatttttgg aaattccttc ttatttttga ggcaaaccac
     9181 ttttgccaca acttcatcgg ggcctttttt tatttgtacc aaaacaaatc actggatttg
     9241 ttacaatcat gaggatataa tttaaaaaag ttttttattt ctatcacatg aatcaatgcg
     9301 ccattaagta gattctttca tgcttcccta ttttttcact tgccgtatcg cttcaaaaac
     9361 aactatcata aactcaaact cgactgaaaa gttattttaa aaaagactaa aaatatccct
     9421 tctggacttg ttcttcttat tctgttatac tagagaaaca cgaaagatat ttttatgcga
     9481 cacttttcat ttgtttttat gaaatttatt caaaactggc gtaacttcag agttacaagg
     9541 atgtattaga ggttagggaa tacatcgttt aaatgagaaa taagtaatag ttagtaatag
     9601 tcacccaaac tttaaattaa tgaatattga atcgtcagat agcctcctcg agttttcgag
     9661 aaggctatcg tttattttat ggagaaattg attcagaagt aatcagcgac cttttttagt
     9721 tcaccaatcg tttctgcttg gaaagtttgc acccaatctt cttggaagcc cctcactgct
     9781 ttatcaatcg agggcatctc taatacttga tctagtagct ctttacctaa cgttatcgca
     9841 tgagctccag atcgaatggc tgtagtgact tgttcacaat ttttgaaact agctgctaat
     9901 atttttgttg gtctttgcgt acgttcgatc tcagtccgca acgaatcaat cactttcata
     9961 gcatcaatgc cttgattttg catcctgtta taataaggtg caatataatc tgctccatta
    10021 ttaatagcta aatacccttg taattctgtg taaatagctg taccggtaat caaataacct
    10081 tcttttttca actttttgat cgcttgaagg cctgcttgat tcaccggcac cttgataaac
    10141 gtatcttttc ccagttcttc tatgataact cgagcatcct ctatcatttc ttcttccgtt
    10201 cttcctattg tctgaatatg gataggaatc tgtccgatta tttttttgat ttctttcata
    10261 tgcgagaaga agtttgacgc tccttctttt ttcagaattg taggatttgt tgttacgcct
    10321 gttaaattca aaaactgttg acaagaataa atactttttg tatttgctgt atcaagcatg
    10381 atttccatat agctttcccc cattctgatc ctcttgatcg attttaatga ttgtcccctt
    10441 tcttaaatgc ggcatttcct tatcttctac atacattgtt cctggaagtt ccggctcatg
    10501 agatccatca aattttatcg tgatatgtcc taattcattc aaattagttt ggacaacatc
    10561 tcctactgct gtgattacat acatttgttt atcaaaacag agcttcatat cacaagttat
    10621 ttcttttctt acctgattca attcaatctt ataacaataa tctgttaatg tttccggtgc
    10681 ttcatctcca aataaaatca ttattttttc tttcctgaat acttctgctt ctataccaac
    10741 atctgttaca accgtttcaa agatcgtcat c
//
LOCUS       DAFBQW010000085        10680 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFBQW010000085 DAFBQW010000000
VERSION     DAFBQW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.088"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(217..423)
                     /locus_tag="LK889_002543"
     CDS             complement(217..423)
                     /locus_tag="LK889_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBK5096740.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1069..1917)
                     /locus_tag="LK889_002544"
     CDS             complement(1069..1917)
                     /locus_tag="LK889_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5096741.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1916..2983
                     /gene="gnd"
                     /locus_tag="LK889_002545"
     CDS             1916..2983
                     /gene="gnd"
                     /locus_tag="LK889_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBK5096742.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2985..3986
                     /locus_tag="LK889_002546"
     CDS             2985..3986
                     /locus_tag="LK889_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBK5096743.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3983..4267
                     /locus_tag="LK889_002547"
     CDS             3983..4267
                     /locus_tag="LK889_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK5096744.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            4300..5097
                     /locus_tag="LK889_002548"
     CDS             4300..5097
                     /locus_tag="LK889_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5096745.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            5111..5944
                     /locus_tag="LK889_002549"
     CDS             5111..5944
                     /locus_tag="LK889_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5096746.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5962..6819
                     /locus_tag="LK889_002550"
     CDS             5962..6819
                     /locus_tag="LK889_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5096747.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6841..7350
                     /locus_tag="LK889_002551"
     CDS             6841..7350
                     /locus_tag="LK889_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5096748.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            7387..7788
                     /locus_tag="LK889_002552"
     CDS             7387..7788
                     /locus_tag="LK889_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096749.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7900..8253
                     /locus_tag="LK889_002553"
     CDS             7900..8253
                     /locus_tag="LK889_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096750.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            8287..8730
                     /locus_tag="LK889_002554"
     CDS             8287..8730
                     /locus_tag="LK889_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK5096751.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8747..9832
                     /gene="uxuA"
                     /locus_tag="LK889_002555"
     CDS             8747..9832
                     /gene="uxuA"
                     /locus_tag="LK889_002555"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK5096752.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9825..10538
                     /locus_tag="LK889_002556"
     CDS             9825..10538
                     /locus_tag="LK889_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5096753.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3608 a         1675 c         2315 g         3082 t
ORIGIN      
        1 tttttcaagg agtaaagtct tatgatgcaa aagaggttgt aatctgtcac ttagctccaa
       61 gtcacaagga gcaatttcaa taaacaagct tttcatggtt tatgaatatt gtaacttgtg
      121 actgaggagt tagagatggc aaggggcacc ttttattcga aatttgggaa tatcacacga
      181 ttattgtcac gatctctttt acgtaaattt tataagtcag atttgttgtt ttgatcgttt
      241 aaaaaaagca acaaatccga agcctgcgga ggcaagaagg gacaaaaagc cgagtggtaa
      301 gcaaaagaat gctggttcca ctaatattcc gttgttattg atataaggct gggaacaaag
      361 atgaatagca aataaaatca atgcactagc gaaaaaaagc aaagaaatat ggatagcttt
      421 cataagcagc acctcctttt ttataattgt atgataaata gattaagaat ctataaatgt
      481 gacaagtttg ttaggatgtg aataaaatga tcatctagcg aacttgaaac aaagatggct
      541 aatgataagc aattaatgat tcagcaatca gataggtaca aagtttacca tcatacgtct
      601 agaccaataa tagaaaatat gggaatataa tagaaaagat ttaggatacc cccaaaagaa
      661 tttccccaaa aattcgatgt atcctaaact tttttatttt acataacaat catatcaact
      721 gtgaaataaa attacaaatg ataacctaaa atgaggccaa aagccagtgt tatcaaggaa
      781 atacttaatt caccaaaatt ttttttaatt ttttagaaaa taagaaacaa aaataataaa
      841 aaaaagaaaa gataaatttt ttactactgt atactaactt tgttatcgca tctaacaagt
      901 aactcgaata ataccaatac aaccttctcc aaagatgcga tgacatacgt tctaacccac
      961 cgactattga tgttctgtac cacccttgca gaaccaaaaa agccactacc aaatatttgg
     1021 tagtggcttt ttttgtttta tggatttttc gctttttctg ttatggtgct attctttgat
     1081 ttcagaaata gtatgtcgaa ctttagcgat agaacgacgt gattcttttt cgttgtaaaa
     1141 catcaaaatc acatatagag agtccaaaat gcttagttga gaaatacggg aagctaaagc
     1201 ttcagatcgg aattctgttt cttcagaaat agaaataaaa acgacatcgc caagtttagc
     1261 aagggaagaa ttagcttggc tagtaataac gattgttttt gctccagttt ttcttacggt
     1321 ttcagcaata tggattgttt cttttgattg ccctgtgtga gaaatcaaaa cagcacagtc
     1381 tttttccgtc aataaagaag cttccatcaa ttgaatatgg taatccgtgc tgtgagaagt
     1441 tgcgataggg gaacgcaaaa atttatgata agcgtcagtt gctacaatct ctgaaccgcc
     1501 aacaccgaaa aaatagacac gtttagattc attgataata gtagccgctt tttttagatc
     1561 cttttgttgt aacaactttt ttgtgtcatt taaagttgaa atactagact cgaacacctt
     1621 ttgcgccata gtgagctcag tatctgtttt ctgaatattt tcgtggatag aaatagcaga
     1681 tagatcattt tcttggatca acatggccat tttaaaatct ttaaatccat tataaccaag
     1741 tttttttgta aattgaaaaa aagtagagtc agcaatgccc agttcacttg ctaaattgct
     1801 aataggacta tgacttactt tgcttggatt ttctaaaata taatcggcga tagcctgttc
     1861 ttttgggctg aagtcgttgt acattgtttt gatttgtagt cttatggaga cgctcatgag
     1921 aattcctcat tttcataata agattatcat tttttccgtt ctcattaatg ttaaagatac
     1981 aaggagagta ggaaaaaatc aaacaaaaaa tatttttgta aaatctattt acaaaaaaat
     2041 ttttttgtat aatgagggta taagaaatgg aggcgctatc atggaaattg gtgtaattgg
     2101 tttgggaaaa atgggattga atttagcttt aaacttaaaa gagcaccatc acaaagtaca
     2161 aggactcgac atttcaagta cagctgttag tcaggcaaga gaaaatgaca ttcctgctta
     2221 tcaagagatc tctgaatttc tagctacttt tgagcagaaa aaaatcatct ggttaatgct
     2281 tcctgctgga gagataaccg aaaacatgtt aagaaaactt tttccaaaac tcggaccagg
     2341 agacattatt attgaaggcg gaaactctta ttataaagat tcaatccgtc gagcagaaga
     2401 gtttcagaaa aatgaaatcg gttacttgga ttgtgggact tctggaggaa tcgaaggagc
     2461 acgacatcaa gcctgcttaa tgattggagg agataaagaa acttttctat ccgtggaaca
     2521 attattcaaa gatgtagcaa ttgaaagagg atatctgtat gctggtgcat ccggaagcgg
     2581 acacttcttg aaaatgatcc ataatggaat cgaatatgga atgatgcaag caatcggtga
     2641 aggattccaa cttgtacaaa aaagtgagta tgcttttgac ttggaacaag tcgcacgtgt
     2701 ttggaatcat ggttcggtga ttcgcggctg gctgatggaa atcatggaag atcagttaaa
     2761 cgaacatccc aatttaagta attatcgggg aatcgtttct gcatcaggtg aagcaaaatg
     2821 gacggttgaa acagcgctag aaatggctgt tccagttcca gtaattgcaa ctagtttgtt
     2881 catgaggaat ctttcacagg aagaagatag tttttcagca aaagtagttt cagcactaag
     2941 aaatgggttt ggcggacatg aaatagttga aaagggtgag taaaatgcca gattatcata
     3001 tagcaatcgt taattccagc agtttcggaa gagtctttcc acaccacttg gaaagattaa
     3061 agtcagttgg agacgttcat ttctttactg ttgatcaaga gattccagga aaagaactag
     3121 cggagttact taaaggattc aatatcatta ttgcaagtgt tacgccattt tttcgacgag
     3181 atttttttga aaacaaagat gaactgctat tgatttcccg acatggtatt ggttacaaca
     3241 atattgattt aaaagcagca aaagaacacc agacaatcgt atctatcata cctgctttag
     3301 ttgaacgaaa tgctgtagca gaaaataata taactaattt gttgtccatg cttcgacgca
     3361 cgacacaagc acaagcaaga gtcaaggacg atcgatggga agatcgggcg gagttcgtcg
     3421 gtaggacgct gtttaacaaa acagttggtg tgattggtgc aggaaatacc ggaagcggag
     3481 taatcgaaat tttgcgtaac ggttttcgct gtgatgttct agcttatgat ccttacaaat
     3541 cagatttata catcgaaagc tttggtgcaa aaaaagtaga acttcaagaa ttactggaac
     3601 aatcagatat catttgttta tgtgcgaatt tgacagaaga gaactatcac atgatttctg
     3661 ataaagaagt cgatcagatg aaagaaaatg tttatctatc taatagtgca agaggcgctc
     3721 tagttgatga agaagcgatg attaatggtc tgaaaagcgg aaagatcgca ggatatgcca
     3781 cagatgtttt agaagaagaa ccgggacgtc aagatcatcc gtatttagcg tttgaacaag
     3841 tagtgattac gccacatata tcggcctaca caagggaatg tttagaagca atgggtgaaa
     3901 aatgtgtcag agatgtcgaa cagatcagtc agcaccttct tcctgaacgt tctgtccaga
     3961 caaagagcaa ctatataaaa ggatgatgaa gaaaatggtg aacggtttaa aagtgaaaac
     4021 aggccctcaa ttttatttat atgaagaagg tggtatttca aaagtttctg atctcttgaa
     4081 atcatacggc gcgaaaagag tactggtcac ccacggaacg gtatcatggg aaaaggcgct
     4141 acctaaatta gtatttttga atgacgaaac gattcaattt ttttatcatc gttattctgg
     4201 agaatgtagt tatgctgaag ccagacgaat agctacaatc atcaaaaaaa tgaaatcgat
     4261 ttcgtagttg gcgttggcgg aggaaaactg accgatctag tgggttatag tgccaatctt
     4321 gcaagaatcg atttcggagt catccctact ttagctagta attgtgctcc atggacacct
     4381 cttgctgtca tgtataagga gaatggacaa gcagaaggga aaacagaaca cttttacaga
     4441 caagctgctt tttttataac agatccccaa ttagtgattg attcccctgt tccatatttc
     4501 attgccgggt tagcagatac actagcaaaa tggtatgaat ctgagctcat cttagaacaa
     4561 gattttttgc aagatgaatc atttttgaag ctagcacaag atactgcgaa gatctgcaaa
     4621 gaagagattt tgaatcattc tgctaaagca atccaagata tggcacaaag aaaactgaca
     4681 tcggagttca aacagctttc agagattatc tttgctgttg ctggtttagt tggaggatta
     4741 ggagataaat acgcaagaaa tgtggcagca catgctatgc atgacgctat cagtaaatat
     4801 attccggaaa gccatcgttt tctacatgga gaaaaagtag cgtatgggat tttctatcaa
     4861 ctggcactag aaggtcgctg ggaaaaaata gacgagttat atcctttgta tcgggaatta
     4921 gaactgccgg tttctctaga acaaatggga ttatttccga aagataaaca gataatagat
     4981 aaaatggctg aatttattga ttcgaaagag aaagtgcatt tgattcctat agaggtaaca
     5041 cagaaatcac tgatacagtc aatgatggaa ctggaaggtt atatcagaaa gatctgaagg
     5101 aggaggacac atggaaacat taagtgtagt acaaagtttg ttgattgctt tatgggtagc
     5161 cgcggttatg tcccgctggt taggtggagg agccacgttg accttacgtt tttctccttt
     5221 gatgactgga ttagtcgtcg ggttgatcat gggggaagtt gcacaagcga tgattgttac
     5281 agccgctttg caaatgattt atatgggggt tttttctcca ggaggatcaa tgccagctga
     5341 accatctatt gcagcagcta tcgcagtacc agtcgcatta ttaggggatc tgtcgccaga
     5401 agcagcgatt gccgttgcgg tgccagtcgg attgctcgga agttacttgt atcagtttcg
     5461 ttttttcatc aatacttttt taggtaaata tacagacaaa gctgtagaag atttgaatac
     5521 aaaagctatg gcccgatcaa ttattcttta tccaacggtt gcctcatttt tattattcgt
     5581 tccgcttgtt ttcattgctt tatatttagg ggcgccattg atcgctgatg taatcaaagc
     5641 tttagaggga acagtcgtta tccacattct tgaagtagta ggtggagggt tagcggcaat
     5701 cggtatcgcg acaactgtat acgttatcgg tcgaaaagat tatctggtct ttttcttcct
     5761 tgcttatttt atgagtgttg ttttgaaatc tttagatatt acgatggtta cttatgccat
     5821 ttttgggatt attatcgctt tgatctttgt ccagtcgcaa aaagggaaac aagtacagcc
     5881 tgccacaagc aataacaatt cagcatcttt tgatgacgaa gatgactatg atgacggatt
     5941 ttaatttagg aggaatggga aatggctgaa ataatggata atcaaacagg tgctcctgaa
     6001 gaaatcacga aaaaagatgt aacaaaagcc tatctgagat ggcattttgc aaacgaaatt
     6061 cctcactctt ttgaacgtta cttggcgcca tcactactat acggaatgat gccaatcttg
     6121 aaaaaacttt ataaagatga agactcttta aaagcagcat ataaacggca gttactgttc
     6181 ttcaacactc aattaagctg gggtggcggt gtgattactg gcctaatgtc ttctatggaa
     6241 cagcaaagag cagaagaaga gtatgaagaa aaagagatct tgatgcagga tgacttgatg
     6301 tataacacga aagcgggatt gatgggggca ctagcaggaa tcggagatgc aatcgattca
     6361 ggaaccgtcc aatatatttt tatcgcaatc gcggtacctt gggcacaaca gggaagtcct
     6421 ttaggcgcac tttttccttt tatctgcttt gggttgtatc aggttttact aggggtattt
     6481 ttcgcaagac aagctttcac tatgggccgt aatgctacag gcctaaagca aaacactggt
     6541 gttcagacag cgattgaaat gctatcagtt ttagggttgt ttatgatggg ggtgcttgcg
     6601 ggaaactatg taaaagtaga atcttcattg aaatttgcga tatccggacg tgagtttgtt
     6661 atccaggaaa ttcttgatca aatcgtgcca gggatattac ctttagctgt cgtaatgggt
     6721 gtgtacttgt tctatacgaa aaaaggattg aaagtcacac aagcattatt atggctgact
     6781 ggaatattaa ttgtgctagc aggaatcggt attttataaa aagaagggaa cgtgaataag
     6841 atgggtgtag tcaatttagc gcgtgtggat gagcgtttga tccatgggca agtaatggta
     6901 acgttatctc aaaaaagtgg agtgaattcc atttttgttg tagacgaagt tgtagcaaaa
     6961 gataaattta tgagagatct ttacaaaaat gcaggaagcc gtacaggaca aaaaacaatt
     7021 gtcattacac cagagaaagc taaattctat tgggatgaat atcaatttaa agaatacaat
     7081 tgtattttga tcgcaaaaac agtccaaacg atttacgact tagtaaaaca cggaatcccc
     7141 atggaagaat tgaatattgg agggattgct caaaaaaatc ctgatacaga tcttttagtg
     7201 acgaaatctg tttatttaaa taaatcagat gctgaaaaat tgaaagaaat gaatgaacaa
     7261 tatggcgtaa agaatattta ttttcaagcc actccatctg cacctaaaac aggattaaaa
     7321 gatgtattga acaagtttga tttgtcataa acaaatgctt agaaaaaaga taaagagggc
     7381 gaaaaaatga agaagaacga gctgtttcgt gattgggaat ttcgttatcg ctatatttac
     7441 cgtaagcgcc gtacaaaaaa gagtaaacag cgttttcttt cagcattagt atcagatatt
     7501 tacagtatga gaacagatgt gacagtcatt gcttacgata cgctagccta ccgatcaaaa
     7561 aatatttatg ttggagatat cgaaaaagca gagaaagtta tctgtactta ttacgataca
     7621 cccgttcatg cattaggaag ttatttcatg tttgattgga aagaccaaag gaaaaaaaca
     7681 atctactcta tattattgtc cttcattttg ttgttcagtt taggttggtg gggaatgatg
     7741 atatataaca aaaatcctca tcacgtattc gatctgctat ccgtataaac aggtatcact
     7801 gtactagcat ttgggagcta ctttttctta ttgggaaaag cagctcgcgg atggcctagc
     7861 agacaaactt ttattcgaaa tacttcatcc atattaacga tgctagaaat gatccgtacg
     7921 attgatgatc cgagtgtagc gtatgcgttt gttgatgaag gctgttatgg agaaaagggc
     7981 ttagacagcg tgagatcggg tatgaaaaaa gaggctattc ttttttatct agactctgtt
     8041 ggggcagata caccgctaca gttttcggga aattattttt caaataaaga acagtggctg
     8101 aaacaagttg ataagttgaa agaaaaaaac gtcaattata tttttagtgc aagaaaaaag
     8161 caagcgcagt tcttctattt gaccaaaaca gatttacgag ggaaaacatt taattggcaa
     8221 aatgccaatc agattattgc tctttttcga taagtaaaaa gtgcaaaaca aggagtgaga
     8281 agactaatgt tggaaatcat gatcgttaca catggtaagt tgagcgaagg actgaaagat
     8341 gcggcggaag taattgtcgg gacatcgagc catattgaaa caatgtgctt agagtctggg
     8401 gaggacatcc aaacattcgg agagaaggtc aaagaaaaat taactatgat caacacagca
     8461 gaacccatgc tagtatttgt tgatctaatt agcgcaagtc cgtacaatca agcgctcttg
     8521 gcagtcaatg aactgactga agaaaagaaa gcagccactt atgtcatagg gggcgtgaat
     8581 ctgccaatgt tgttagaagc aatcaatcat cagatgcttc acacaccgat agaagaagcg
     8641 gtggaacaaa ttgtcaaaca aggaaaagat aatttaactg tttggcatgc ttcagcggaa
     8701 gaaaaaaaat tagatgagga cgatttttag aaggaaggct gaaaagatga agtggggatt
     8761 tagatggtat ggtgaaaaag gggattcgat cccattaaaa tatgttcgcc aaataccggg
     8821 aataaatggg ataattggaa ctttgctgac taaaatgcct ggagatgttt gggagatttc
     8881 agaaattgag gagttgaaag catctgttga gagccaagga ctggaactgc ttggaattga
     8941 aagtgtagcg atccacgatg caatcaaggc agggacagat gaacgagatt actatattga
     9001 taactatata cgaacaatca aaaacttgtc tcgatgtggg gttcgattga tttgttatag
     9061 cttcaaacca atttttggat gggcgaaaac tgatttgttt aatgaaaaca acgatggaag
     9121 ctattctctt ttatacgatc agaaaatcgt cgatcagatg gagccaggag aaatgtatca
     9181 attgatacac agccagtcaa agggattcaa attgccgggt tgggaagaag aacggttgaa
     9241 aaaattcgct cagctggaaa aactgtatga aggaatgacc gaagaagaat tatttgaaaa
     9301 tctgacttat tttttagaaa aagtgattcc agtgtgcgaa aaagcagatg taaaaatggc
     9361 tatccatcca gatgatccgc catgggaact ttttggtttt cctagagtga cgaaaaatct
     9421 tggcgacttg aagaaaattc taaatgcagt agattctcca tataatggac taacgctgtg
     9481 cacaggatct ttaggggcaa atccggaaaa cgatttagtt gaaatcatcc atgaggtagg
     9541 ttcgcgcatc cattttgtcc acttcagaaa cgtggagttt ttaggagaaa gaaagttcaa
     9601 agaaacggca catccctcta cagaaggctc ccttgatatg tatcatatta tgaaagcgtt
     9661 ggtgcaagaa gggttcgacg gtcctatacg ccctgatcat ggtagaaccg tgtggggtga
     9721 agtggctatg cctggatatg gcctgtatga tcgtgcaatg ggactcactt acatgcaagg
     9781 tctatatgag gcgataagta aggaaaaacc actgggagag cattatgaat aaaatcaagc
     9841 tcttcatctt tgatatggat ggccttttat tagaaactgg acgtttagct tatcgtgctt
     9901 atgtaaaatc agcacaaaag tatgattatg aaatgcgtaa ggaagtctat tatttactaa
     9961 ctggccaaac agaaatggcg ataagagaac aaatgggcat tttgtacgga gaagatgttc
    10021 catatatcaa atggcgagaa gcaatcaatc aatataaaga aaaaatcgtg aaagaagata
    10081 agagagtata tacgaaaaaa ggagcagaag aaattttatc ttttgcaaaa gaaagaggaa
    10141 tccatacaat cgtggcttcg tctaatacac gggtaaaagt agaaatgtat ttaaaaatgg
    10201 aaaatctata tgccttgttt gatcaaatca tttcagggga tgatgtaaaa aaaggaaagc
    10261 cggaaccaga gatctttttg aaagcttgtt caaaaatgaa tattcctcca agtgaagcac
    10321 ttgtatttga agactcgatt gcagggatcg aagcagcaag gcgagcgggc attttgtctt
    10381 ttctcatcaa agatcatttg atagggttgc ccgatcataa tgggaagcat aagttgaaag
    10441 tagatgtaag tttatatgaa tccaataaag cagactatga attttatgac ctgcatcaag
    10501 caaaacgatt tttagaaagt aaagggttgt atctctaata aagaggacta ttttctagtc
    10561 ctctttattt attgtcctgc acgactgagg gaacagatga aaaagaagta attttttatt
    10621 ttgaagtatc taccttattt gtagtgaaca atttagaaca aacacaaaac gcatctactg
//
LOCUS       DAFBQW010000086        10662 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFBQW010000086 DAFBQW010000000
VERSION     DAFBQW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.089"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            335..2281
                     /locus_tag="LK889_002557"
     CDS             335..2281
                     /locus_tag="LK889_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5096754.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2284..2739
                     /locus_tag="LK889_002558"
     CDS             2284..2739
                     /locus_tag="LK889_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5096755.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2753..4081
                     /locus_tag="LK889_002559"
     CDS             2753..4081
                     /locus_tag="LK889_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK5096756.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4115..4399
                     /locus_tag="LK889_002560"
     CDS             4115..4399
                     /locus_tag="LK889_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5096757.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4401..4916
                     /locus_tag="LK889_002561"
     CDS             4401..4916
                     /locus_tag="LK889_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBK5096758.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4932..5594
                     /gene="rpe"
                     /locus_tag="LK889_002562"
     CDS             4932..5594
                     /gene="rpe"
                     /locus_tag="LK889_002562"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK5096759.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5601..6173
                     /locus_tag="LK889_002563"
     CDS             5601..6173
                     /locus_tag="LK889_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5096760.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6360..7880)
                     /locus_tag="LK889_002564"
     CDS             complement(6360..7880)
                     /locus_tag="LK889_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK5096761.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8123..8308)
                     /locus_tag="LK889_002565"
     CDS             complement(8123..8308)
                     /locus_tag="LK889_002565"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBK5096762.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8292..8474)
                     /locus_tag="LK889_002566"
     CDS             complement(8292..8474)
                     /locus_tag="LK889_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096763.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8715..8933
                     /locus_tag="LK889_002567"
                     /pseudo
     CDS             8715..8933
                     /locus_tag="LK889_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9428..10027
                     /locus_tag="LK889_002568"
     CDS             9428..10027
                     /locus_tag="LK889_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5096764.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10091..>10662
                     /locus_tag="LK889_002569"
     CDS             10091..>10662
                     /locus_tag="LK889_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HBK5096765.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         3526 a         1675 c         1989 g         3472 t
ORIGIN      
        1 attaggactc aattgttcat agatgagcat ttgtaattaa gttatattat aataaaaaaa
       61 tacgaatttt agtgacgcca ttatattgtt tttatttttc aattgtaata tttcatatta
      121 cttccataat aaggagtatt ttctattgga atatcttagc tcttttaagg atttctcctt
      181 ttttgtttct cattttttaa gattatttgg atttccttta caacaaactt ccacaagtat
      241 tattgacata gatgtaattg agggaagggg gttccagtgc tagaataaat aatgaaaaga
      301 ggtaacaaat gctagcgtta agaggtgaac aagtttgatt gaaagacatg taaatttatt
      361 aaaattatta agtcgacaag aagactttaa acccgcttct ttttttagtg ataaattggc
      421 agtgtcgacc aagaccattt atcaagatat tgaattgttg gaagattatg tagttaatta
      481 ccaagtaaca attgagcgat taccttcttt gggtatcaaa ctcataggtg ataggaaaaa
      541 cgttgaacat ttgatttcat cggtagaaaa ttatcttgaa aaagatattt attctccaga
      601 aaaaagacgt gtcgagatca ttaaagaagt tttgtttggt gataagcctg tttctttaac
      661 tcatttgtct caaagtttta tggttagtaa aacatcgtta tacaatgacc tgagaattat
      721 caataaaatt attggcagtg gagaagttaa gttgcagtca tctgcagaag gaattagttt
      781 tactggtaat gaacaagcaa ttcaacgagc aatcaagcaa cttgtattct attatgcgaa
      841 tgacatggaa tcaaaaacat tttttcaagt tttaaatatg ttgtttgatt caaaagttat
      901 tagtgtcatt catgatttgc tattcaatga ttacagtgaa ctgactgaaa aggtttctga
      961 ttactatgtt cggtcattga tttctacttt agttatccaa tgtaaccgtt tactgaaagg
     1021 tcatcattta actgttgaag aagaattcct cttcaatagc attcgctata tggaaacgta
     1081 cattgtggca aatggtttaa ttgaagaatt gaaggcaaaa ttgaatcttc cattttatga
     1141 cgcagataaa gaatatctat gtcgtcaact atttgcacat cgtgtgacaa ataatttaaa
     1201 aaccagcaat gaagagtacg ctatgagcgt tcataaattg attgaaagaa tgagtgagat
     1261 tgaaaaaatt gatttgactc aaaatgatcg attatatcaa tccttattgt atcatgtgcc
     1321 agcaatggtc cttcgtttaa aaaaaggtat taaaattcaa aatccgttat tagaaaatat
     1381 caaatctcaa tattcagaac tctttagcat agtatggtac gccatgtcca tccttgaaga
     1441 agaatttgat gtgactttaa cggacgatga agtttcctta attttgattc attttcagat
     1501 tgctattgaa gatcagtcca aagcaaataa tatcatcatt atttgccaat acggtatttc
     1561 ttctgcgcaa ttaatttaca ataaagttcg acggttttta ccagcaaaag ataatgtaga
     1621 aatatcaact ttagataagt ttcatttagc tgaaattggg gaagttgatt tgattatttc
     1681 ctctctttcc tttgatgcag gtggtatacc cttagtaagg gtatctccat tggtaagtaa
     1741 aacagattat atgaatatta tggaagctta tactaaatat ttaatagaag aaccttcgcg
     1801 atttattcaa aatgaactga ttcctaaagc ggttaaagtt cccaccatat ctaaatttgt
     1861 tcaatcatct ttagtaaaaa caggagtaca ggtggaaagc aaagaagaat gtttggattt
     1921 gatgatttcc gaactagaag ccaattcatt agttactgaa gagtttcgtg cttctatctt
     1981 ctctagagag aaaatgggga atactaattt agaaagtggt gttgctttac cgcatgcgaa
     2041 tccatcaatg gtcattgaat ccaacatttt tattgtagca ttaaaaaatg cgattaattg
     2101 gggcgatcga aaagtcaaat tagtcattat ggttagttta gctgaagaag atgtatctga
     2161 gattagagga gtatatgaag aactttacca atttattggg caaaaagaaa tggtcgatcg
     2221 cttgttacaa gttcatgata gcgaaaaatt actaagaatt tttcaacaaa aaggagaata
     2281 aaaatgtatc tagataaaga attatgttta tttcaattag aagtgaaaga tcaaaaagaa
     2341 ttgtttcaag taatgtctga acaattaaaa aacgctggtt gcgtgaaaga tagtttctta
     2401 gaaggtatca ccaaccgtga acaagaattt ccaactggat tagaagtaaa tcaaattggg
     2461 tttgctatcc cacatacaga ttccgcacat gtaaatagct ctcaaatctg ttttgcctca
     2521 ttaaaggaac cactggtatt ttcagatatg acagataaat ctcatgaaat acctgtacgt
     2581 ttagtcttta tgttggcaat gtcacaacct catgaacaaa ttgatacact acaaaactta
     2641 gttagcctat ttcaaaatga agaaaaagta aatgaattac ttgcatgtac cacgaaagaa
     2701 gcatttattg agttagtaca ttctgcagga gtttattagg aggaatagag agatggatat
     2761 tgtattaaat gcctttgaat ggtttattgg attagggtca aacgtctttt taccactgat
     2821 tatttttgta atcgggatat tatttggatt aaaacctggt aaagcatttg tttccggcgt
     2881 tactgttggt atcggttcta ttggtttagg attagtttta agtttattat cagatggttt
     2941 aggaacagca attcaacaaa tgggggaaca atacggtggt ggcctgaata tcctagatat
     3001 tggcgtaggt gttggtggac ctttggctta ctctacatca ttgggaattt taatgattcc
     3061 aatttcatta attcttaact tcatatttgt ggttttgggt tggacaaaaa cattgaatgt
     3121 tgatatttgg aacttttggt tcgcaatttt cctaggtatg attactcaat ctgttacggg
     3181 aaacttttgg tttggcttaa ttggcgcgat tgtcgcagta attttacaat ggttcttagc
     3241 agatcgtaca caaaaaactg ttagcgaatt tttcggctat ccaggaatct ctatcacaca
     3301 tatgatggga ttatcaggag tactatttgc caaaccagta aattgggttt ttgatcgtat
     3361 tcctggattt aataaaatag atgcagatgc agaaacatta acaaaacgtt tcggtgtttt
     3421 aggtgacaca gttgttattg gcctaattat cggtattgta gtgggtatta tggcaggcta
     3481 tgacgtagca ggaattggaa acttaggaat tatgactgcg gcagtgatga agattatgcc
     3541 taaaatggta gcaatgttta tggaaggttt aatgccaatt gcagaagcgg cgaaagaatt
     3601 tacgaataaa aagttaaatg gtcgccaagt aaacattgga atggatgcag cgttaactgt
     3661 gggtcatcca actattatgt caacttctct attgcttgta ccaatctcat tattgctagc
     3721 actaattctt ccaggaaata aagtattacc ttttggagat ttagcattct ttgctttcgc
     3781 catctgttta atgattcctt attttaaagg aaatattgtg agatcattag taggatgttc
     3841 gttgtatgtt gttacaacat tatatatggc aacatggttg gcaccaatta tcactgatgt
     3901 gtttacatta gcaagtttcg atgtggggac agcaggatta gtaactatgt tgttatgtgg
     3961 attatggcca gtagggttgt ttgttttatt agcatcttac tttggtgtaa ttggaatagg
     4021 tggattagga gttgttttag taggtgcttc tatctacatg aacaaagtga agaaagctta
     4081 atcataaaat taaaataaga aagaagagat caatatgaaa aaattattag ttatttgtgg
     4141 agcaggtcat gctacttcta caattgcagt agcaaaatta tcaaaatggt tggaagaaga
     4201 aggattaaca gatcaagtga aaatttatca atctaaaatt gctgatgagc taaataaaat
     4261 tgatgaatat gatgctgtag taagtacaac aattgtccct gatgtaatta aagctaaagt
     4321 aacgaatggc ttgggcttat taacaggtat tggtacagat gccatttatg cagatgtaaa
     4381 agaaagatta ggtgtttaat atgcgttttc atcatgacac tttgactatt gaaacagttt
     4441 cagggcgtcc ttcgtatcat gatattcgaa aagaactttt agatacagtt gatacagcag
     4501 gtattgagaa tggaatgtta gtaatttcga gcccgcatac aacttgctca ctcttttttg
     4561 aagaaacgat gcatgacaca aacttttttg gtgatgatta tttacatgta gacatcaaca
     4621 atgtaatgga aacgattatt ccaaaaatga cgagtgaaaa tcaatacaac agtccggggc
     4681 caaaacacat tgattttgga acaagattat ctgatcccaa ttaccctgca gaaaaatggg
     4741 tcatgctaaa taccgatgca catttacgtt catctattta tggaaccaac tcaatgactt
     4801 taattgttca agaaggtaaa cttttattag gagaaatggg ccgtgtctat tttgtcgatt
     4861 gggatcaact aagagagcga actagaacaa tcaatctcat gattatggga gaataaacgg
     4921 gggaatagag aatgacaatt gctccatcat tgttaaatgc acctatcgca gaagttaaag
     4981 agttatttga aatcttaaaa gaaaagaatg ttgattattt gcatattgat gtaatggatg
     5041 gctgttttgt accagatatg gcttttggac caagtgttat taaagatatt cgtccactaa
     5101 cagacgttgt gtttgatgtt catttgatga ttgatacacc agaaaaaatt gtcacaagat
     5161 ttgtagaagc aggagcagat attgttacct tccacattga agctaccaaa cacgcaatga
     5221 aggtcattca gttagtcaag gaagcaggag caaaagtagg gattactttt aatcctgcaa
     5281 caccagtcag ttttattgaa ccagtattat ctttagtcga tcaagtatta gtaatgacaa
     5341 tcaatccagg aacagagaat aagcatttta ttcaagagac agtagttaaa atcgaacagt
     5401 tagatgtaat acgcaaacaa aatgattata cgtatgatat tgaagtagat ggcaagattg
     5461 ataatcaaac gattaaagtt tgttctaaag caggggcgga tatttttgta tcaggcggtt
     5521 tcatcttcaa tgggaaaaat accccgcaag aacaaattga taaactaaat caatcattag
     5581 aggaagtgaa gtaaaagatc atgaccaata aagaccaaaa taaaaactat ttagaagcga
     5641 ttatcttaga attacaaaat aactcaaaac gtattaattc tgtagagtta gaacaattta
     5701 gtgagaaaat tgttcaagct aaacacatct ttctagctgg tgcaggacgc tctggaattg
     5761 ccatgcaagc atttacgaat cgcttaatgc atttaggttt ttcagtcagc cttgtcggtg
     5821 aaattagttc accacattcg caacctggag atttattaat tatctgttct ggctcaggtg
     5881 aaacaggtag tttaaaaaat ttagcggcca aagcaaatca aagtggtgta agcattgctt
     5941 tagttaccat gaaaaaagac tcgtcgattg gtcaattagc agattctgtt cttgtattac
     6001 caggtattac caaagatgaa aatcaacgag aagaagatac tttttcacaa cctatgggtt
     6061 caagttttga acaattagca tttctcacct ttgatagttt agtgttatct ttaatggata
     6121 aaacaggtga aagctcagaa acgatgtttg ctcgacatgc agattttgag tagtagattt
     6181 taaagataaa aaaccattct ctaaatgcta attattcagc aaatatgaga atggtttttt
     6241 tatttgatta gacataactt aataattcag tagttttgag cgtaagcgtg tcatagaatg
     6301 gtacctaaac acaaatagcc cgatttgata cactcaaatc aggttatttg tggcttatat
     6361 tatttacaga tttcttgtat ctgctgattc catggcaaat aagcgcgtag cgcttcaggt
     6421 gtcatcgaag acagattggg aatctctgta aaaagatgat ttaaatattt gcggatgttt
     6481 aacccattcg caacagctgt ctggataata ctcaatataa ttccatttga tctagcgcct
     6541 tttaagcttt tagaaaatag ccagtttttt cgaccgatca ctaactcttt gatggcacgc
     6601 tctgctcggt tatttgaaag ttccaagcgt ccatcaagca acacaatctg aagtccgtct
     6661 ttatgattga ctgcgtattg aaccgccttt ccaagctgcg attttggcaa ataagcaaaa
     6721 ctctcacacc aatgaaaaaa ttcagtcatc acaggctgca acgtttcctt cctcgctaca
     6781 aacctttctt ctgcgctaag cttattaaag gtccgttcga ttcgaaacat cttgtcacag
     6841 aagtctaatc ctattttggc atgacttttt ggggacggtt gtttacccaa tgcttcaaaa
     6901 aactttctgc gaacatgcgc ccaacaataa acattgttta ccggcagctt tccataggcc
     6961 gcgtaaccat cagtatgcaa gaaaccgcta aatgattcta aaaaactttt tggctcatca
     7021 acggaacgcg aatttgcgtg ctcgtagagc acaacttgtt tttcttcgct tttacctgaa
     7081 gcatataacc agtaaaaggt cttttcttta tgagtattca atacccgata gcttgtctca
     7141 tccgcgtgta agacctcctg attgcttaat tcttgatgaa ggcaattgta gattggctct
     7201 aaaaccattt cacttacctt gatatgccaa ttagtgattt ttttacgatc aagagggaaa
     7261 ccaagtgcat gccattcttt ttcttgtcga tatgccggca cctttttaac aaatttttcc
     7321 caaatactct gtgtcacaat tgaagaggaa cctagactat tattgatcaa cggtttagga
     7381 acaggcgctt ttttgatttt tgtttctcct aatgcttcac attctctgca atgataggca
     7441 tgttggtaga tgacttgttt ttctatatgg gcaggaacat acaatggctc ttctctaagc
     7501 cttgtcgcac cgatatcatg taaagtatga ccacagtctt cacacaaaca atcggcacct
     7561 tttaattcat aatgaatagg aacttcaggc agaccttcta atgaaagtgc tttgagtccc
     7621 ttgcgttttc tgcgtttaaa agaagatacc gtaatgggtt cctggctttc ttgtccagtg
     7681 tgctctggtt cattaaaagt tgagtcgtct aataaatcga tttgcccatc tacgggtggt
     7741 gtttcttttt ttcgtccaaa caagcgtcga ttcatttcag caatttgttc tctaagaaga
     7801 gtattttcct gttgctggat ttcaagcaac tgtgttaatt gcttgatttg tacaagaagt
     7861 tcagattcca tttttgtcat ttttacacca ccattctagc gattttctta catctattat
     7921 accattttat gacaacgaaa aacagacaaa atccttgatt taacaaggtt ttcgtctaat
     7981 atatcgcacc ttttttcgtt tcttttagcg tacatttttc ttcgattgaa aggccagtca
     8041 gtaagacttc ttttctggaa cgcggccatt gaaggtaccc actttcaaat cttttgtaca
     8101 ttaatacaaa tccattttga tcctagtaaa gagccttgaa gcgatctttc ttcgtaccgc
     8161 aaaataagaa aagcgcttga tcgtaaacat caaggtcata ttcttctata acgaaagctg
     8221 ctagtccatc aattcctttt cgaagatctg tttttccaca aacgataaaa atatgtttca
     8281 atgcagttgc atcaatgagc attggcattc aactctttta acaaggtata caaaagatat
     8341 ttatccattc cattataaat aaatatttcc ttatcttccg atttgattct gcaagctagt
     8401 tttggcttgg tgtcaatttg cgatttttga gccgcacttg aaaattgaac tggtacaata
     8461 tcttttttat tcattcgttt ttctccttct atatttgata gtttaattat agaagaaaga
     8521 taatcggcgt acgagatact atcctatgac acgcttacat ttaatttaat attttgcctc
     8581 aaattagcca cactttttat agaactaaga ggaaaaatga agttttcttc ctattaatat
     8641 agttgtttga aatcttaaat atagcgtttt acgatgtaat gggaagtatg gtgaataaat
     8701 caactatgtt cgtacgaagc atttactcaa ctaatttaat cgaatctttc aataaacaaa
     8761 tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg gaacgtttct
     8821 tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat aaaggtttcc
     8881 aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat tagaaacaat
     8941 ctacagtagg gaagaaccat tgacacaaaa ttattgacgc tcccaactaa aagtgtcaaa
     9001 ataatgatga aatcatttag ttcttataaa ttagaaaaga gaaaataaat attccatatc
     9061 tactttattg gatccgtaac atgactaaat tctgaatact ttttaaagct tatcatttgg
     9121 taaaactgaa tataagttaa ttatatagac tgaaaatttt gaattttcag aatagaataa
     9181 cttaaaagta ctgacaaaat tttttaaagg ggcaatatta atgttctgag ataatcaaat
     9241 cctaccgaaa atatcaaaaa tccaatgtga cacaatcaga ttgtgtctca ttcgagggaa
     9301 tcacacaatc ccacgtggct ctaggcagaa ttgaattttt gcttggggct ttttccttat
     9361 attgattttg aatcagactt attatatgtt ataatgcaat taagtatcat tggagagggc
     9421 gaagaaaatg aagacggtag aacctattcg agacaaaaaa aagatcgatg cgatgaaggc
     9481 aatcttggct tcgggaaaat atggccaacg aaatttggtt cttttttcga ttgggatcaa
     9541 caccgcctat cgcatttctg atttgcgtca attgaagctg gccgacgtgc tggaaatttc
     9601 acgcggtcgc gtggtggtca aagatcgatt agcgatgaag gaacaaaaaa cagctaaaca
     9661 taattcggtc tatatttcca acaaattgcg aaaaacgatt ttggagtacg ttcaacagga
     9721 atttcctgat caactccttc agcaagattt tgacggttat ttgttcccta gtcgaaaagg
     9781 gacgaatgaa ccgctcaacc gccaaagtct gtggcgaatc attcatgatg ctggcacggc
     9841 aatcggattg aaagacattg gcccacattc catgcgaaaa acgttcggtt actttctcta
     9901 taaacagggg accaaaacag aaatcattca gtccttgttg aaccattctt ctcagcgcga
     9961 aacattgcgg tatattggta ttacacaaga agacaaagat acagctgtaa aaagcttaga
    10021 tttataatat aaaagttaag tttaaacatg gatagtttta aattagtaga ttgagtgttg
    10081 ggaggaaaga atgagtacat tatatattat aggaaacggt tttgatttaa gtcatggttt
    10141 aaaaactaga tacactgatt tttcaaaatg ggttgaaaaa agtcatagtg aaatattcga
    10201 taaaatagac acaatgataa atgaaaataa tgaggtgaga agtacagctg atgataagat
    10261 aggttggttt aattttgaaa gtagtttgca tgagttaatt caaagtccag tctttgaaga
    10321 ttatgtagaa gagtctaagg attatctcat aggttatgga catgaagatt ggtcagattc
    10381 tgcccatcat gattttcaaa atatggttta tgattatgtt gtttctctag aagaattaca
    10441 acaacttttc aaggaatgga tacgcaaaga aatacagccg acaatagaac ataaaattcc
    10501 aaaataccaa ttttcgtccg atgtactatt ctttacattc aattatacag atgttttgga
    10561 aattctatac aagcaaccga aagaaaacat atatcatatt catggcagtt atgacgatat
    10621 aataatcggc cactccgaga attttgacaa ctttatagga ga
//
LOCUS       DAFBQW010000087        10362 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFBQW010000087 DAFBQW010000000
VERSION     DAFBQW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.090"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            174..323
                     /locus_tag="LK889_002570"
     CDS             174..323
                     /locus_tag="LK889_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBK5096766.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            334..1848
                     /gene="dltA"
                     /locus_tag="LK889_002571"
     CDS             334..1848
                     /gene="dltA"
                     /locus_tag="LK889_002571"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBK5096767.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1845..3050
                     /gene="dltB"
                     /locus_tag="LK889_002572"
     CDS             1845..3050
                     /gene="dltB"
                     /locus_tag="LK889_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBK5096768.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3095..3328
                     /gene="dltC"
                     /locus_tag="LK889_002573"
     CDS             3095..3328
                     /gene="dltC"
                     /locus_tag="LK889_002573"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBK5096769.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3331..4599
                     /gene="dltD"
                     /locus_tag="LK889_002574"
     CDS             3331..4599
                     /gene="dltD"
                     /locus_tag="LK889_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBK5096770.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4740..5822
                     /locus_tag="LK889_002575"
     CDS             4740..5822
                     /locus_tag="LK889_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBK5096771.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5822..6625
                     /locus_tag="LK889_002576"
     CDS             5822..6625
                     /locus_tag="LK889_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK5096772.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6695..7435
                     /locus_tag="LK889_002577"
     CDS             6695..7435
                     /locus_tag="LK889_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5096773.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7446..8450
                     /locus_tag="LK889_002578"
     CDS             7446..8450
                     /locus_tag="LK889_002578"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBK5096774.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8495..10339)
                     /locus_tag="LK889_002579"
     CDS             complement(8495..10339)
                     /locus_tag="LK889_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096775.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3338 a         1708 c         2090 g         3226 t
ORIGIN      
        1 tttttaaaag tacgagaaat tataaagaaa aaagcagtga tttgatgtgc tatttttata
       61 aaagctatgt gacaaaactg ttagtaaaaa aacggtttct gtaagctaga cacatgtttc
      121 tttttgtcaa aattagttac actttgtgtg aggttatatg aggagggaat ccaatgaaca
      181 aatggcgttc taatccaaaa ttgaattatt ggggaaaatt catcattcga acaatttttt
      241 atagtgcagt actgctgttc ctgatttatc tgtatcacta taaaaatatt caaggaggaa
      301 cttttatcta taacgaattt taggaggaag agtatggaaa tcaaaacgat tattgaagca
      361 atcgatgatt ggggaataaa tgaacctgat cgagttgctt atcaggcgga tgagacgcat
      421 acttttggtg aattgaaaca agcatcagac gcacttgctt attatttaga aaaaaaggta
      481 gaaggcgatg gaccaattgt cgtttttggc aatttggagt ttgaaatgat tgtctcattt
      541 ctaggggttg tcaaatctgg ccatgcctat ttgccgattg aagagcatac acccaaggag
      601 cgtatcctat cgatctttcg ggtggctaaa ccatctatgg tcatcagtat cggggactgg
      661 atagaagata ttccttcggt tcctgtcata acaaaaaaag aatttcaaga aatcaagaag
      721 ataccagtag gattttatcc cagaaatagc gtaaaaggta cagaaaattt ttatattatt
      781 tttacttctg gtactactgg agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc
      841 agctttgttc aatggacgat ggaagacttt gggatcaagc ctggtatgca tttcttagcc
      901 caagcgccat tttcttttga tttatcggta ttttctattt atcctgcatt agtttctggc
      961 ggaatgctga agccgttaaa taaagcagtt gttcaagact ttcgtcaatt attcgcgaca
     1021 ttgccagcct tgaagttgaa tgtgtgggtt tctactccat catttatgga tatttgcttg
     1081 atggaaccta cattcaatgg agaaaatgtg ccggaattgg acatgttttt attttgcgga
     1141 gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc gattcccaaa tgcacggata
     1201 tataatactt acggtccaac agaagcaact gtagctattt caagtattca gattgaccag
     1261 catgtactag atagttatga ccgactaccg attggctacg taaaaaaaga cacgaaagtt
     1321 tctattgtcc aagaaggtaa acctgtttca aaaggagaaa cgggtgaaat cattattgca
     1381 ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa agacagctgc tgcgttcttt
     1441 tgtcaaaatg acttagcaag ttaccataca ggggatgcag gtagactaga tgaatatggg
     1501 cttttatttt atgaaggacg tatggatcag caggtcaaat tacatggata ccggattgaa
     1561 ctcggggata tcgaacatta tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta
     1621 ccaaaatacc aaggaacaaa agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa
     1681 acgaaacagc cggattttca gttgactaaa tctatcaaag aacaattact agaagttgta
     1741 atggattata tgattcctca gaagttccat tatgtcgatc tgctgcctca gacagttaat
     1801 ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc ctgaatgatg ttacctcata
     1861 tgatccctta tgcagagccg ttttattttt ttcttttagc cattgcgctt gtaccgatcg
     1921 ttctatcttt attgatatgg gaaaaaagat taccagttta tcaatcagca gtgacacttt
     1981 tctttttatt tgtcagcttt ggaggtaatt attggcaaca gggagcggcg ctgattgggt
     2041 atgttttgtg gcagacgata cttgtatggg gctattttgt ttatcggcaa aggaaaaatc
     2101 agacaggatg gttttttgcc gccgttattt tagcgataat tccactgttt ttaacgaaag
     2161 tgactccttt ttttgaacat ggaaaagcat cgttacttgg ttttttagga atctcttatt
     2221 taacctttaa atctgtacaa atgatcatgg aaataagaga tggcatgatc aaaacatata
     2281 agccatttcg ttatatccag tttctgctgt tctttccaac aatctcttct ggaccaatcg
     2341 atcgctatag gagatttgaa aaagatcagc tccatccgcc tgttcgtgat aaatatgttg
     2401 atcttttggg aaaaggcgtc caaaatattt ttgtcggttt tttatataaa ttcattatcg
     2461 gttattattt tggtcaagta ctgcttcctg tagtgggacg cattgcctta cagcatggtg
     2521 gtatttcatg ggcattagta ggttatatgt acgtgtacag tatgtatttg ttttttgatt
     2581 ttgctggcta tagcttattt gcagtcggta caagctacat gatgggatat cagacaccaa
     2641 tcaatttcaa taaacctttt ttatcatgga atatcaaaga attctggaat cgttggcata
     2701 tgacgctgtc cttttggttc cgtgattatg tatatatgcg attgatgttc ttcatgatga
     2761 agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat cggttatttt gctttgtttc
     2821 ttctaatggg tatatggcat ggattgacgt ggtactacat cgtatatgga ctgtatcatg
     2881 cttgtctgat ctgtctgaca gatgcatgga tccgatttaa gaagaagcat aaagagcagt
     2941 tgccttctaa caaatttacg catggcttag ccgtattttt gacatttcaa gcagtatgtt
     3001 ttagcttcct gattttttca ggatttttag ataaactttt ttttagctaa cgaatagtga
     3061 catattaatt tatataacaa aggagaaaag gaaaatggaa gaacaaattt tatcaatctt
     3121 agaagaaata acaggaacag atgaagtaag aaaaaatcgt gatattgatc tatttgatga
     3181 agggttgctt gattctttag catctgttca attattagtc gagctagacg gagagctgga
     3241 tatccaagtc cctgtatctg aattcgagcg agaggattgg agcacaccga ataaaatcat
     3301 tcaacaagca actgcattga agggttgagt atgaaaaaga aactttttgc tattttcggc
     3361 ccggttgttt tagctgttgg cttactgttt gtgtttttta cctcctccta tcaagtagat
     3421 atctctaatc cagaaacgat tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa
     3481 ggagatgcga taaaaaatta tgcattatct gaaaaacaat atattccttt ttttggctca
     3541 agtgaattaa gcagaatcag tccatttcat ccgtctgttc tggcagaaaa ataccagcga
     3601 aactatcgtc catttttgct gggagcaccc gggacacaat cgttaagcca atatatgatg
     3661 atgcgttctg caggagatgc tatgaaaaac aagaaagtcg tctttattat ttctcctcag
     3721 tggttcgtta aaaatggtgt aaaaactgat tactttaata cgtattattc tgaacttcaa
     3781 acatacgact ggttgttttc aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga
     3841 agattattaa ctttctcaaa agtaaaagag aatgatacgt tgacggcgat tctgcaaacg
     3901 atcaaaaaag ggaagctacc tttacctgaa tctttgaatc agctgcgcag ccaatggaac
     3961 atgctgaaga gagaggatga agtattcagc aatattgggt tgaaagatcg acagcaaaaa
     4021 atcgatcatg agtcaaaacg attaccaaaa caataccaag aaacagaatt atccatttta
     4081 gcaaatcaaa ttggagaacg agaaacgacg aataatccat tcgggttgaa aaacgatttt
     4141 tatacgcatc gtatccgagc ccacgagcca gagctcaaac agtcacaaaa aaattgggat
     4201 tatcgttttt cgccagaatt ttctgatttt cagctggtct tggatcaatt agcaaaaaac
     4261 cataacgaag tattgttcat catcccaccg gttaacgaga aatggagcga ttataccgga
     4321 ctatctcaag aaatgttaca aggatttgcc aaaaagatca aatttcaatt aaatagccaa
     4381 ggattcaatc gaatagctga ttttgtcaat caagcgggta ccaactattt tatggaagac
     4441 acgatccatc taggctggaa aggctggttg gctgcagatc aacagatcag accgttttta
     4501 gaagaaaatc atattactgc tagtaagtat catcttgacg atgctttttt tagcaaatct
     4561 tggcaacatc aaataccaga taaactacaa ttaaaataaa gaaaacaggc gagtgttgct
     4621 cgcttgtttt ctttattttt gaatattctg aataaaagat aaaaatacca agtttgcctg
     4681 aacaaaaatc ataagcaatg gtataatcac aatatgaata tagaaatggg ggaaaacatt
     4741 tggatacaaa agaagaacag ctacttattg atttaacttc tgcaaaagga gtacctggaa
     4801 atgaagaaga ggtaagagaa gtattcagag aatatgccaa accattcgct gatgatattt
     4861 tttatgacgg tttaggcagt gtgattgcca aacatggtgc tggcggaggc cccaaggtct
     4921 ttatttctgg acatatggac gaagttggtt ttatggtaac aaaaataacc gaaaaaggat
     4981 tcttggaatt ccaaactctt ggaggttggt gggggcaagt catgcttgct cagcaagttg
     5041 aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg
     5101 ttttaacacc tcaagtaaga aataaacctt atgaaataaa ggatatgttc attgatattg
     5161 gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg tcctggagat atggtcactc
     5221 catatattga atacaaacga atgaatggca gtaaatattt actggcaaaa gcatgggata
     5281 atcgtatcgg gacagctgtt tctctgcgag tattagagaa tctatctaaa gaagcacacc
     5341 caaatgtttt attcgcagga agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa
     5401 caagtacaca ccttgtaaat cccgatattg cttttgcttt agatactggt acagcaggag
     5461 acactccagg aatgacacca aaagaagcag attcgatttt aggaaaaggc ccacagattt
     5521 tgatttttga tgcatctatg atcccgcata aaaaattatt gaattttgtt attgatgtag
     5581 cagaggaaat ggatattcca ttccaatata cagtgatttc tggcggagga actgatgcag
     5641 gacaaatgca tttgacaaga gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt
     5701 atctgcattc ccatacatca gtgattcatg aagatgatta tttgaataca gtgaaactag
     5761 tcacagaatt agtgaaacgg cttgatagtg aaacagttga aaaaattcgg agttatgaat
     5821 aatgaataat agaataccta tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa
     5881 tcaatgggga atcaatactt ggcttgtttt cttatctata atatttacga gcgtcatcca
     5941 gaactttttt gacggtttca gtccttttcc ggaagaaagc atgcagcagc acttattgag
     6001 ttttctagtc gataatttac ttttattcgc attcaactat gcattgtttt acattgcgct
     6061 agtagttgta cggggtggca gaccaaaaag taacctttta ttttcagttt tccaaagaaa
     6121 gtattttggt tcgatacttg gcatcaattt gatcaattca gtcatcaact ggctactcaa
     6181 cagtttaatc tttctgccca tgtttttaat cagcggagtg agtacttata tgcagctgct
     6241 tttcagttca aatgcggtac ctacgggtgt catgccagaa gattttcttg atatcagctt
     6301 tgttttatcg atgataggga tgatttttgt ttcagcactc atcatgagta ttgttggcgg
     6361 tttattccaa tttgctgcat ggacaaagtt cgactatcct gatttgtcgg ttattcaatg
     6421 tttgaaatat ggctggtttt tattgaaaga tagaattggt acgtatattt tgttgcagct
     6481 gtcttttatc ggctggtatt tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt
     6541 cattgtttat gtgaatgtgt cagttgcaga attttatgaa caagcacgga ttgaaaaagg
     6601 aggtccagac gcctatttta cttagcatgt aagcagttta ttcatcaaaa aaataacttt
     6661 tgtggtagga taaatctttg agggggaatg tacgatgaaa ttaactgttt taggatgctt
     6721 aggagcgtat ccttataaaa atcaagggac aacaggttat ttgcttcaat ctgacgggtt
     6781 caatttgttg atcgatgcag gtagtgctac gctgatcaaa ttacaagaac accttgatcc
     6841 acttgatcta gatgctgtga tccttagcca ttatcatcat gatcatattg ctgaccttgg
     6901 tgtattacag tattactggc aacttcatcc taagagaaat ccggagacat tgctttcaat
     6961 ttatggacac acaaaagatt ccattcactt tgaagaattg acgttggaaa atgtctcaaa
     7021 aggacatcct tattttgaag ctgaagaatt aaaaattggt ccttttctga ttacttttat
     7081 ggaaacaatc catccagtag tatgttacgc catgcgtatc gtagaagaaa caacggggaa
     7141 agtattcgta tttacaggtg attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga
     7201 tgctgatttg tttctagcag atacctatct ttttgaagga aatgaacatc atcatgccca
     7261 tttcacttca aaagaagctg gagaacttgc gaaatcagct aacgtaaaga aacttgtact
     7321 tacacaccta cctcagtttg gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc
     7381 aggcaaaggg attgatatag aacttgctgc agttgacaaa gtgtttaaca tttaggagga
     7441 agaacatgat ttttgtacca aatgaaaacc acgacccaag aatcaattta gcaattgaac
     7501 aatatttgct gcaagaaatg cctttagatg aacctatttt gctcttttat atcaatgaac
     7561 cttcaatcat catcggcaga aaccaaaata cgcttgaaga aatcaatcga gattatgtgg
     7621 aagaaaatgg cattcatgtt gtccgtcgct taagtggagg cggagctgtc taccatgatt
     7681 ttggaaacct gaactttagt tttatcatgc ctgatgatgg ggattcattc cgtgactttg
     7741 cgaaagtaac acagccaatc atccaagcac ttcatgaatt gggtgttgct ggggcggaat
     7801 tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg
     7861 ctaccaacgg tcggatgttt gctcatggaa caattatgtt cgactctgat atcaatgaag
     7921 tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc
     7981 gttctcgtgt aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa atgacaacag
     8041 aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa
     8101 aaacgtacga attgactgat gaagattgga aaaaaatcaa tcgaatctct gaggaatatt
     8161 atcgaaattg ggattggaat tacgggaagt ctccagattt caatttctct cgccaaaaac
     8221 gtttttctat cggttcgatt gaagtccatt tgaatgtctc agaaggaatg atcaaagaag
     8281 caaaaatctt tggcgatttc ttcggactag gagatatctc tgacgtggaa aaaaaattgg
     8341 ttggacagaa atatgataaa gcatctctat caaaaatagt cagccaaatt gatgtgaaga
     8401 aatattttgg tgcgatagaa ccagaagaat tgcttacctt actttattga aaataaaaaa
     8461 tagcggaatc cacatatatg gattccgcta ttttttatac aggattcggg taatttcgag
     8521 agagttgaac agtgatccga tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt
     8581 aacgatccgc tacgtctttc atcatcatta agtcaaaacg ttcctgatca tctaattctt
     8641 ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc actatgttcg caacaaatga
     8701 cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt tcgatacagt ggattttcga
     8761 tggcatgagt ttctctaaag gaatgctcta aagcgtggat caaaagttga gccagatcgt
     8821 catcgttgat aggaagttct tcgggaagat ctgcttggaa ggttatctga atattccgtt
     8881 tggcagctaa cttctggtaa cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag
     8941 aacacagcat ttcgaaattt tttgcttctt ctaatatctt ttccacatag tttttagctt
     9001 ttgtttggtc cgtatgcatt aaatcttgca gattctccat ctcttggaca aattccaggc
     9061 gaagtgtatt tacttcttgg atatgctgtc gaagctgctc ataatgttgc tccaacaaat
     9121 catttttgat ttttaagaaa tctacttgtc tccgatcttt gtcggtattt aagatgtgat
     9181 cgattagatt gtagctgata ataacgatta gaatgataat atataaaatc gtagaccaat
     9241 tgatcgttgt ttgatagata cctaatgccg tttggatgta aatcaagcaa tggataatgg
     9301 ctaccaagac gatccaaggt gtacaagcta cataaaaacg gttattctta tatgcttcgc
     9361 caatgcatgc aagtgaagtg actaaagtgc ttataacact agcaatatta gcaatcggca
     9421 ttaattcagg aagttctgca ggagtaaaag ttgcataaag cagaacgccg ataaaaatca
     9481 aaagttggaa cgtacaaaaa attccgtacc attttttata gtaaaacatt cggctccaat
     9541 agtagcagat gatgaaagct gacgttaaga taataaataa atgagataaa aaagaatgaa
     9601 cgaatggttc aaaaagaata ccacttaaaa tatcttctga tacagcttca aatcctaatg
     9661 ccaaagcaaa gcaacttaag agaataagtg agtattcaag acgtttttgt ttatacatca
     9721 agtagatcgt atagaaaaag gtccctatag cgataaaaac agcgattatc atcgtcaata
     9781 cttgcggcac tgattgtgaa taaataaaac taatcaatgg tccagttgtt cctagataaa
     9841 ctttcggtgg cagtccagca tagttctgat aaggagatga gactgtaagg gtcaattttt
     9901 ttccgatata atttgctggt aaatctatca cagccaatga gagtccggga ttgctttctt
     9961 cctgataatc tttccgttgg tataaaatct tttcgcctag atatacagta atccattgat
    10021 ggtttccttt gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa
    10081 tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa
    10141 aatcaggatc atcttttgta tagaaaaccc acgaatcttt tatttctttc atttgattgc
    10201 tgtccgcagg agaaaaaata aaataaaaga aataagcaat tcctgttaaa aataacaatg
    10261 ataatataat gattatgctc cactttatac gtaaataaaa agattctttt ttattatata
    10321 tgttattaaa aaaagacata aaggtattcc tttctaaaac tt
//
LOCUS       DAFBQW010000088        10298 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFBQW010000088 DAFBQW010000000
VERSION     DAFBQW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.091"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(12..392)
                     /locus_tag="LK889_002580"
     CDS             complement(12..392)
                     /locus_tag="LK889_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5096776.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(389..775)
                     /locus_tag="LK889_002581"
     CDS             complement(389..775)
                     /locus_tag="LK889_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096777.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(777..1055)
                     /locus_tag="LK889_002582"
     CDS             complement(777..1055)
                     /locus_tag="LK889_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096778.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            1103..1831
                     /locus_tag="LK889_002583"
     CDS             1103..1831
                     /locus_tag="LK889_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBK5096779.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(1977..2453)
                     /locus_tag="LK889_002584"
     CDS             complement(1977..2453)
                     /locus_tag="LK889_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBK5096780.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(2413..2760)
                     /locus_tag="LK889_002585"
     CDS             complement(2413..2760)
                     /locus_tag="LK889_002585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096781.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(2747..2932)
                     /locus_tag="LK889_002586"
     CDS             complement(2747..2932)
                     /locus_tag="LK889_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096782.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(2933..3205)
                     /locus_tag="LK889_002587"
     CDS             complement(2933..3205)
                     /locus_tag="LK889_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBK5096783.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(3202..3405)
                     /locus_tag="LK889_002588"
     CDS             complement(3202..3405)
                     /locus_tag="LK889_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096784.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            3530..3742
                     /locus_tag="LK889_002589"
     CDS             3530..3742
                     /locus_tag="LK889_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096785.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(3775..3969)
                     /locus_tag="LK889_002590"
     CDS             complement(3775..3969)
                     /locus_tag="LK889_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096786.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(3977..4165)
                     /locus_tag="LK889_002591"
     CDS             complement(3977..4165)
                     /locus_tag="LK889_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096787.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(4162..4302)
                     /locus_tag="LK889_002592"
     CDS             complement(4162..4302)
                     /locus_tag="LK889_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096788.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(4792..5157)
                     /locus_tag="LK889_002593"
     CDS             complement(4792..5157)
                     /locus_tag="LK889_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096789.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(5197..5499)
                     /locus_tag="LK889_002594"
     CDS             complement(5197..5499)
                     /locus_tag="LK889_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBK5096790.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(5499..5798)
                     /locus_tag="LK889_002595"
     CDS             complement(5499..5798)
                     /locus_tag="LK889_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096791.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(5791..5955)
                     /locus_tag="LK889_002596"
     CDS             complement(5791..5955)
                     /locus_tag="LK889_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096792.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(5961..6230)
                     /locus_tag="LK889_002597"
     CDS             complement(5961..6230)
                     /locus_tag="LK889_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096793.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(6242..6991)
                     /locus_tag="LK889_002598"
     CDS             complement(6242..6991)
                     /locus_tag="LK889_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096794.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(6994..7680)
                     /locus_tag="LK889_002599"
     CDS             complement(6994..7680)
                     /locus_tag="LK889_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096795.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(7686..8357)
                     /locus_tag="LK889_002600"
     CDS             complement(7686..8357)
                     /locus_tag="LK889_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBK5096796.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(8350..8691)
                     /locus_tag="LK889_002601"
     CDS             complement(8350..8691)
                     /locus_tag="LK889_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096797.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(8869..9567)
                     /locus_tag="LK889_002602"
     CDS             complement(8869..9567)
                     /locus_tag="LK889_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBK5096798.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            9654..10124
                     /locus_tag="LK889_002603"
     CDS             9654..10124
                     /locus_tag="LK889_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096799.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT         2873 a         2084 c         1570 g         3771 t
ORIGIN      
        1 ataggggggc ttcaattgaa atacgaagga aaaacttttt tgtcttccgt ttcgttttca
       61 acaattggat gacattttga acataatagc atgagattgt ttggatcaag cttaagcagt
      121 tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc tctttccaaa tatgaactga
      181 ccacaccgct gacaatgacc accttctctt tcataaataa attgacgcat atctttccat
      241 gcttgcgttc gatagaatgg tttgttctca tgatgataaa cagactttgc ttgctgcttc
      301 ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt agataccctt tgctattttg
      361 ttcgtgcatc cgtcaaactg acagtatttc attctacttc acgaataaga tttatgatat
      421 cgccttttgc acggatagca ccaggaatat caataccatg tttcttagca tatgcacgca
      481 attcttttgc agtcatattg tccagttcat ccgtatcttc agtggactga tcattagtag
      541 cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct atcaagaatt tcactgctat
      601 gagctatgag ttcgccattg acagcaacaa atgatttgcc atcactcaaa attccttttt
      661 cttcttttac ttcaaaattt ggttcttgat ctttcggaac aactactgtc cttttctttt
      721 ctgaatccca atactctgtt ccactaatgg atgttctaat tttgatcatt gccattttaa
      781 ttctctcctt tgtaatttgt acggattact tttgcgccca ttcgctcata ccattcaatc
      841 gtttcctgca gattgggtag agtgtgtgac aataaagaaa taactaacgt attcattcca
      901 gttctgtaat catcattctc cacttgcaca tgtgcatgat taccattcca attttctctg
      961 tacggactat tctctgcttc aattgctttc ttataaactt tagccaaact tgattctact
     1021 tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt tactctccta tcagatattt
     1081 tgtggtatga tttataaaaa agatgcgagg tgttttaatg cttcaagaag ttaatattaa
     1141 gtatcgtgat cgtcaaaata cagagacaat ttatttgcca aatagttgtc cgcattgcgg
     1201 aaatataatg actccgtatg tgtattttgg ggtttcatca tatagtgcgt tcgataatga
     1261 tagaatcttt ggcgtattag tacagtgtat aaatgatgag tgcaacaaat tttatagttt
     1321 acaatttata aatacgttta atgaagatga aggatattct gaaaatattt taattaaaga
     1381 ttattaccgt ccgccgatta aaatagattt accagaaaat attgaaaaag tttctcctac
     1441 atttgttgat atttatactc aagccacaca agctaaatct gagcaattgg aacaaattgc
     1501 tggagttggt tatagaaaag ctctagaatt tctaattaaa gattatgcaa tatctaaaaa
     1561 ttcagaggat gaagataaaa taaaaaaaat gttattaggt aaaacaattg aaaattattt
     1621 gaatgatttt cctaaactcc aaagattagc caaagctgct acttggattg gtaatgatga
     1681 aacgcactat gttcgtagac atactaataa agatgttcat gatatggatg catttattaa
     1741 atcagcagcg caatttatcg cggctgatta tgatgctgat ttagctgatc aatttataaa
     1801 tggaaataac tttatgccaa ctactaatta aaagaataaa agagccacag ataccaatta
     1861 ataaaacggt tgggatatct tggctctttc atattttttt gacactaata gaatatcatg
     1921 ctgctaacaa tgtttacata gctacaatga ccgaacattt agtgaacagt gtaattttag
     1981 aataaagaga gttgttcttt gtacttctcg ctattgtaaa aatcaatttc gttttctttt
     2041 ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa agttcaatgt ctttctaatt
     2101 tctgcatgtc gctgtctgac atacgatgag ctatacccaa cagattcagc aatatcttct
     2161 aagctcattt gatcaacata tttcatctta acaatttgat tatcgatacc agaaaagcta
     2221 tctatcagca ataacatttc ttctttttgc tcgatcaaca attccaattc attttctatc
     2281 ttttggatat tctcttctaa agaagaagtt ctcgaattct tttcgatgcg gacgtttgct
     2341 aaatcaccac agacccatcg attcaattca agcttacttt tattaagatt ccactttaag
     2401 taaagaatct gctcatctag ttcttggtaa tcttttaacc aatgaaatct cacaaacgcc
     2461 accccttatg gtagaatagt attgcgctgc tatctcgaaa ggggtagctt ttttattttt
     2521 tcttagccgt tccattctgg attgcctttt gttccaaacg tcgttttttc ttcttaatct
     2581 ttgattttgt ttttcccata caattctcct ttccgaaaac acattttgtt cgatttttaa
     2641 tactaaataa aagttggcca gaatttttcg ttcgtcttta ctcatctttt tttgaataag
     2701 attactagca taaaaatagt tagtaccacg aaaatgaaca ggtaaatcaa ttacgttcac
     2761 cttcttcatg ccagttatca gccactagaa tctttgcgtt agtgccgcca ccgttttctt
     2821 ttttgtattt ttcaaatgct ttataagcag ctgtttttga tttatagatt tttccggaac
     2881 cgactgggaa catccatgtg ccctctgtga tagctaagat gtatactttc atttaattca
     2941 cccctttaat ccccaacact acatatccgg acttctgcat gtaatcagta atataagtga
     3001 tctctgcttt gtatgagtca ccagtgtaac taaatgttgg tctcgttagt ccaggtaatt
     3061 catgtttaat gtatttttta tattcacgta gtattaactg atcaccaact ttgaagtcac
     3121 gatcattctt gcggatctcg aattgtttgc gtccgctcgt cactgcttca aaatattctg
     3181 gtagtatttt tagttcgtgg atcattctgt ttcctccttc aaaattcgct catcatcaaa
     3241 aagtgaatct ccacaaaatc cttctcccaa atatttatcg ttcatgtata agtcaaccaa
     3301 atatttgtga tctgcttctc gatattcatt aatattaacg atctttattt tgtaaacatt
     3361 atcttcgtcg ctcactatct tgaattgttc tctgactttc accaccgttc ctcctctttt
     3421 tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag cttttttatt tgatataata
     3481 cttgtatcga ttaatcaaat cgaaattaaa tttaaaaagg tgatttatca tgaatatcaa
     3541 caatttagta aaactcgttg aacaacgttt taaagaatca ggattagaac aattaaaaaa
     3601 agataattct ccagaattat tttctaattt taccgaagac gacattccta ctcttaaaca
     3661 attaaaatat tattctgatt attggacgca aactcgtgag aatgaattaa atttagaaaa
     3721 actcattatt aaaaagaaat agcaactcca gcccacacgg ctggtttttt agtttcaact
     3781 tccctcctta cgggtcaaaa tcatcggttc cggcataatc tgatcatcct ttagcaacaa
     3841 atcaggatat tttttcctta gcttcttatt gttcgtgtaa gttttcaata gccattggtt
     3901 ggcttcgctt ttgctttctc cagaaaaata aattttccct gtttggatgc ctgcaagttg
     3961 aatcatcatt ttcacctcaa atcaaatttt tttctgctag atggagcaaa agcaatccga
     4021 catcaagaat cttttgaccc attatttcag ctactttgct cggcttaact ccttgagaaa
     4081 agagcaagcg tgcttgctct atctcttcgt gagtccaaat aaatttggct tcctcaagga
     4141 taattactgg atcagttgct ctcattgttc ctctggaaaa atgatgtcat tcttatgctt
     4201 ggaccaggta tctgcatatg gaacaaagta ttgtctagct aattcgattt gatcaattaa
     4261 agcagtttct gataattcat ggtctgcagc aatttcattc atactatcgc cttcatctaa
     4321 acagataaga ccgttgacgt tatcgatttt tacatttgtg actgcttgac tttctccacg
     4381 ctcataatat tctacagttg atactgatac atcagcttcg gagtttaatt gcagaatctg
     4441 ttactgccgg gataatttaa tattcttttc ataattcacc ttttttccta ctgacatcga
     4501 atatttttca tgttatgatt agccgtgaaa ggagagctat attatgtgtt tacacatgat
     4561 gtccttagct cattcggttc tttggaagct tttttggaaa taactctcag gttcttgacg
     4621 caaatctcat ttattctttg aaacaaaaaa cttccatttt ccgagagctt tggatcactt
     4681 caaaagagat ttgcatttat atttctgtga ccttgagtcg cgtaaagaac cttacagcaa
     4741 tggcggcaat tccactttca ggaaaccgcc ttttttcatt gatattggtg gttagcggac
     4801 tacccatttt ctttcggcag tgccaaacca caaatgatct gtgttgatgt ttaggtagtt
     4861 gtataccaga taagctaaat ccaaagtgcc tgcatcttca agacgacaat attccaaaaa
     4921 ttctcccata gctggacata aaaatcctgg agctatcata tcccaacagt taatagtgca
     4981 aagaaattcg tttagcttct gaacgttttc tttggtttca cgaattacaa catactccaa
     5041 ttcttctccg ctggcagagc attccaaaac ttcaaattga ttaagaaatg gtagaacata
     5101 tgcttcaaac atcaacattc catctttctg tttttgcact tgttttgcat caatcatgtc
     5161 tatcctccta ttcagttatt tcttccatta atggaattat ttcaggtcac tggacttaac
     5221 gaaaacacca ttgaccatct ttccagtccg acctttgatt tcgtcataag caaaatccaa
     5281 gcactcgtat aaatccatat cattttgcat tgccaagatg atcaaggtta ctactacatc
     5341 gccaattccg tctcttaaac catgttcatc ttttctagct agagaagcgg caacttcccc
     5401 gatttcttcg accgttttta acatttgctt gctgaaatcc gcttgatcca atcccttatc
     5461 ttttgcccac tgctctactt tcgcgattag ttcgtccatt atttctcctc cacataccta
     5521 aactgtcttc cttttgaatc aatccataag ctcctagctc tatcccaaat aatatttttg
     5581 ctcagaccag taatttcaga taactgttta gcggtacccg ttactagaat tcgatcacca
     5641 tgccagattg caatctttct cggcgttctc cgtttgggct tttcagtcca cattgattta
     5701 ccgagctttt ggacttctgc aactattttt ttatcttcct gccaatcttc tgaataagtc
     5761 aattcgataa ttcgctgcat tgccgctttc ttatccatcc cgacattctc ctttcagtaa
     5821 tttgagtact tgatcaagtg cgctctcacg tccgccatga aatgtgttga gccacttgtc
     5881 ttcgtacgac acactttgtc ttaaagcttc ttgatgcatt aattcgatct gtgctgtaaa
     5941 tgtctttagg tccatcttgt ttacacctgc tcaagttcat taagatgttt ttgcaagcct
     6001 ttaacacaat caatgaatag taatttggta tatgctaaat ttcttaattg cgttccatcg
     6061 atatagagtg cgaaatagta tctgagttta ctccaactcg aacgatcatt cttaattcct
     6121 tcaattccag cttcttcgag ttgatcatat acgtctctca ggacgtctaa ttcctcgccc
     6181 tttttataat tcgctatttc attaattagt tctagataat cgatcttcat ttttccacct
     6241 cttagaatgg tgcttttgat tgtctattag ctcgttctag cgcttttttc ttgagatagg
     6301 cttcttggtc gattgcccat tcaggaagct tctctcgtct tcctgtgcgc ttgtatccac
     6361 tgcttgcgtt cttaggttca cttttttctt tccttgccca acttcgaata gttgccaaat
     6421 agtttttata agtcttacca gatgattcac aatactctga cagtcgttcg attcgctctt
     6481 ggtaatcatt agggaattct gttttgagtt tctccatctg ctcatctgac aaaagaacat
     6541 ttttatactc tccgtattta tgacggacgg gcttagcctt cgattttttc gaaggcggta
     6601 actctcttat atattctttt gtattattaa atgtattatt aatagatgta ttattatctt
     6661 tgactttttc gtcaataggg gtattgcgtt tttcgtcaat agggtattga ttaattcgta
     6721 ggtacctatt gattatttga ttggtaccct ctttgtaaat gatttcccga ttcaagtatc
     6781 caaacttaat caaatcactt acccatcgcg atatggtctc tttattcaca ccatataaat
     6841 ctgcaaagta ctcattgcct gcccaacaaa agcctctttc attacacaag gccgttatct
     6901 ctccgtataa caacttagta tttggtttaa gtcttttgtc gtaccttacg ttggctggta
     6961 taatcgcata ataacttcga tgttctgtca tttttaccct ccaatattta actttttgat
     7021 tgttttctgg tttaatttga tccctttgat ttgatattta tttttgaaat taatcacacc
     7081 tattttgtgc ttctctgtgt gatggattct gcagagtgct gcaaatgtgt actctgcatg
     7141 atcaacttct ttgcgctttc gtcttcctag cgctttgtca aagtgatcga tgtcagctcc
     7201 tgttttgcca cagatgcagc agactctttt tgtgatgcat ttgtagaagt aatattcttg
     7261 attcgcaggt aaaatctcat agccttcttt gaaaggaata tgatgttcaa agatgaaatc
     7321 taagatgata tttgctaaga cattggcatc actcacagtt gtattcgatt cgtctttcag
     7381 gcttattttg cgccctgtga caccttcaaa acgaaagtag aagaattcct tccagaagtc
     7441 cgttggcatg cctgtatcga taaaaatatc gcctatcagc gcatagatga agtttcgttg
     7501 ctgtacggtg aaacgtctag gatcaataaa acgaatttca atgactcgat caccatcgta
     7561 gccgtcatac atcgtcttta gtcgatcaat gttcacttcc tcattgatgg ttgcgcttat
     7621 gtcttttcct ttgaactttt tcagaaccgc tgaatatgaa tcgattaatg gtttaaacac
     7681 tcatatcact tcttatctaa ttcttttctc ttagctgcta ttgctcgctc catcaaggca
     7741 cattgctcat agcttaactg ttcaatagtt tcaacgttat cagctaagag ccctaattta
     7801 tctgtctgct cattaacata ttcgattaag gttttggtca tatctttacc catctgctca
     7861 ttgaaagctt ctagaatcgt ctctagcatg tttaatttct ttgtatcgat tctaggtggt
     7921 gttggaatat cttccccttg aaatacatat aatcccagtc cgtgtagagc caatgctttc
     7981 acaaagcatc gcttcaatga gttattgatt tgcattgcat ttggtttaac aactggttgg
     8041 tttcgataat ctaaaacagg aaataattcg gtttccgtgt gtcctttaac cgttactgag
     8101 acagatacat aagtcccagt ttcatccata agaaaaggtt tatattcctc aacaagaaag
     8161 tcttgatgag ttccagaaac aaccctgtag tgtttgtact cattaatagt taccgttgcc
     8221 tgtggatcat tctttttcat aatctcccac gcgtgagccc aagataaata atcaaaattt
     8281 ccttttttct tgagaatttt atttaactta cgactaaaaa gtttttcaaa gttcgttgtc
     8341 cctttgattt cactcatcaa attctgcctc catttcagca atgtatttct tacctggtcc
     8401 gtaataagag atatcaatca agttatctct gtcgtactct tctagcgcat caatcaagcc
     8461 atcttcgatg acgtaaatat attcaggttt attcgaatgc ttcgatagat ggataagata
     8521 aacatgatcc caaatagtta catggttgcc taaatcatct tgatcccaag ctagttcttc
     8581 attcgtcaaa agatttcgtc tgatttttcg accacttgtt tcctcaattt tcggcttgcc
     8641 ccaatcagga tcaatcaaat attgatctag agtggaaagt tctttttcca tatgctaaaa
     8701 tctccttatg atgtgttttc tttgtgactc tttgcttgcc ggcggagtca cttttttatt
     8761 tgttgccatg ctttttgctt gtcaatatgt tgttggctta ggatgattgg tttatagtat
     8821 ttccaccagc aattagcaat tgccgtccct attcttagcg cttcagctct attcattgtc
     8881 atcaccaaaa agtctctgtt gtctgttcag ttgatcaatt tccatacgga tcgcagtttc
     8941 tggcaaccac atttcaataa atgaaacagc atcatcgaat ctcttacgag gtaactcgcc
     9001 atatcttggg attgaaaagg tacgtttaaa ttcagaccaa aattttgaga atactttttt
     9061 gctgatttct tcataagctc ggctttcttt tccccctaga actcccataa ctttcatatt
     9121 tcctttttgc ttaatttcaa actcttgttg tccgctaatt cgcatagtat ctttaagcat
     9181 ggaaacatct tttttaacat ctttcatttc ttctaattga tagatcatca tatcttcaat
     9241 tgtttgagga acagtattct tgcgaataac atcttccatt tcgttgaatg cttcaatgta
     9301 tttttgtttg aagtagatag ctttctttcc tgtaaacccc atagccagca agaaaaatcc
     9361 atctctacta atgaagaaaa ctcgtcgatt tctgccatat gaatctggtt cattaccttc
     9421 tacaaacatc tgtccaaaat tggacacatc tttttttagt gcatcaatat ctcttaaaac
     9481 atgttgatgt tttttctcga agctttctgc cacttgtaag ctcgtagtca cagcttcttt
     9541 atttttcaaa attactaatt cttgcattat ttcttctctc ctttttggta taatttagtt
     9601 aaaaactggt ggtgtttatt ttgaattttt ttcacttaac aatttttcaa tggatgtcaa
     9661 taataagttt tatattgagt acgtttttaa cagggataaa aataaaagga atgcgccctc
     9721 aactagaagt tgaacttaat gcttcttact tcgctgcaga tatgatttat acaaaagtaa
     9781 tcatttctaa ctattcaaca gaaccagcta tgttagttaa tttagaactt tcatccgcta
     9841 acttaaaccg cagatggtca gctactccat ttaagaaatt aattgctaaa ggaggatcag
     9901 tcgatgataa tagaatatac tctgaagctg taccgctaaa tattcctcct aaaagtgcta
     9961 tatcttgtta ccttgcattt gaagtaggga aaattaattt taataaactc cttaatcacc
    10021 aaacaaaaat gatatttact ctaaatagaa cccaaatcca caaaattgtt gatatcaaaa
    10081 atacaaactt cccaatagag aaattagtga aagaattaaa ctgagtaata gtaatataca
    10141 ttctagtttc tcttttctat tcatttttag tcagccccct cggttggctt tttcgctctg
    10201 tactcagctt cagcttcatc aagccccata aaaatccaaa ccatgtaaac aatcgttcct
    10261 atcaacgctt gcctgcttcc ccaaagtcct aaagcgta
//
LOCUS       DAFBQW010000089         9563 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFBQW010000089 DAFBQW010000000
VERSION     DAFBQW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.092"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..197
                     /locus_tag="LK889_002604"
     CDS             <1..197
                     /locus_tag="LK889_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096800.1"
                     /translation="QELKRAEQERPDLSFFFVIEGKTIKDLQTRNRQLMRSSGILGLK
                     PLYKKELIKILYRMNNMNDE"
     gene            219..2243
                     /locus_tag="LK889_002605"
     CDS             219..2243
                     /locus_tag="LK889_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK5096801.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            2243..3442
                     /locus_tag="LK889_002606"
     CDS             2243..3442
                     /locus_tag="LK889_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK5096802.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            3458..4102
                     /locus_tag="LK889_002607"
     CDS             3458..4102
                     /locus_tag="LK889_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096803.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            4113..4919
                     /locus_tag="LK889_002608"
     CDS             4113..4919
                     /locus_tag="LK889_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096804.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            4942..5171
                     /locus_tag="LK889_002609"
                     /pseudo
     CDS             4942..5171
                     /locus_tag="LK889_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5192..6265
                     /locus_tag="LK889_002610"
     CDS             5192..6265
                     /locus_tag="LK889_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBK5096805.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            6309..6578
                     /locus_tag="LK889_002611"
     CDS             6309..6578
                     /locus_tag="LK889_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096806.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            6799..7086
                     /locus_tag="LK889_002612"
     CDS             6799..7086
                     /locus_tag="LK889_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096807.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            7104..7526
                     /locus_tag="LK889_002613"
     CDS             7104..7526
                     /locus_tag="LK889_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096808.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            7546..9138
                     /locus_tag="LK889_002614"
     CDS             7546..9138
                     /locus_tag="LK889_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096809.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            9394..>9563
                     /locus_tag="LK889_002615"
     CDS             9394..>9563
                     /locus_tag="LK889_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096810.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDA"
BASE COUNT         3544 a         1471 c         2000 g         2548 t
ORIGIN      
        1 tccaggaatt aaaacgggca gaacaagaac gtcctgactt aagcttcttt ttcgttattg
       61 aaggaaagac aatcaaggat ttacaaacta gaaatcgtca gttaatgcga tctagtggca
      121 ttttagggtt aaaaccactc tacaaaaaag aattgattaa gattctttat cgaatgaaca
      181 acatgaatga cgaataaaaa agatggagga aatgattcat gctagatgtg ataaaaacag
      241 ctttttcaac gcaaagtaat caaaaggtga ttgatccatt aaaacaactt ggtaatcgtg
      301 aaatagagat tttaaaatca aaaggttatg atttagattt cttaaaacaa gtgatgccac
      361 aaggtgggtt gaattttgat gaagaatatg tgacctatgg aaatggttat tcacgatgtg
      421 tgacagttta tcattacaca aaaaatccta ctcttttttg gttggccaat ctcatgaaca
      481 atcctcatac agtggccaca ctagatgtta gcacagatga aaagcaaaaa gtgattgatc
      541 aaatcaaccg cgcattagat gaattaaatg atcgatcaga taatgaacga aaagcgactg
      601 accgaaataa aagtgcggaa gaatacctgg aactagcgga atatgcccga aaattgactt
      661 ctaatgggga aatttctaaa caagtcaaag tgagagtaca tgtttacgga aatacgatcc
      721 aagaagtgga tgaacgggcg caggaactga tcgaaaatct aaaagggatg gattatcgtg
      781 cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc atgttcgatg
      841 aacaagctga atggtttgga aatatcaaag ggcaatcact tccatcgtta aacattggcg
      901 gaggattccc gttccaccac caaacgctaa aagatccagg aggcacacaa tatggggaaa
      961 ccatgacagg cggcccattt gtctggaatg ccacgtataa aaatagtgta cgtttatcta
     1021 ccaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt aaaatgatcg
     1081 cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga aaagacttta
     1141 ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct gacgggatga
     1201 tcaatccttt agagatcttt gccacacgga cgtatgatga agcatccagt ttatatgacg
     1261 atggttcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaacg cacttagaca
     1321 aagtagtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat
     1381 tcaagaccta cttgaatcgt ttttatgaag agtatggaac agtaccacgc ggttttactt
     1441 cctcaaattc gcgttcaaat actgatacgc aagtcacagg gaaagatcca gaagcttatc
     1501 cgacgttcaa agaattttta gcctttttag gacaactgga actaccaggg gcttcccaag
     1561 agaaaaagaa tcggaaagaa gaaatggagt ctatcgtgga atccttatgt gagacttatg
     1621 gcatgatttt tgatggtcat agtacgattc gacaccttga ccaacaacag ttggtctttt
     1681 ttgacattga ctctattaca ggcctagatc gaggggtaca acaatgtcag atgtacatgg
     1741 ccatgacgtt gatttggaat caggcattga ttcaaggacg aagacaacga cgcttaatta
     1801 aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt gatgagtgcc
     1861 ataatgcgat taatccagat ttcattgaaa cggtaaatta tgtcacaaac ttccaacgag
     1921 aaatgcgtaa agtagaagca atgactgttt tagctacaca atccccacaa gaaatggtgc
     1981 cagaaaatat gtcttctgat aacttctcga aactgaagaa agtctttgaa ttttcttcta
     2041 cgaagatctt tatgcgtatg gatgaatcta ttttacgtca tatagaagga ttagttggta
     2101 aatcaatgac gaactcagaa ttagaatcca ttcctcaaca aaatcaagga gacgctgtga
     2161 tcaattttgg ctcaaaagaa accattcgtg ttcattttag tccgaacaaa cgtcaattaa
     2221 aattgtttga tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca aacagtatgt
     2281 taagcgtcgt gcaaagcggc gcttattttt gtgggtgttt ggtacttctg caggtttgat
     2341 tactatattg atcactgtgt ttgtgaccct ttttctaata cttgcggcag gttctattga
     2401 taattctgat tcaagtagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc
     2461 catttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgctgt
     2521 aggtgcagca gtgaagtata aattattacc aagtgtgatc ctttcgcaat acggttatga
     2581 atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc aattattttg ggattacgtg
     2641 gtttgatggg tgtctgtttc ctaaaggaac tgcgcgtgga attggtggca ttgaaggtgg
     2701 ctggtacatg aagtttccca actcaaaagc tgccttctcc tattacggct ttatggtagc
     2761 cacacaatca aattttaatg cttgtgtcgg aaataaaagc cctggcgcaa gcttattgat
     2821 cttaggacga ggagggtatg ccgccgcagg aattactgaa gacagtgcct attataaaaa
     2881 ttgtatgtct attattaata caaataaatt gatggagtac gatgagtttg cgattaaaca
     2941 ctggagagaa ggcggcggaa ctggtggtac gattacagga agctggggca atccattccc
     3001 aggaagtagc ttagataaaa attcgttcag tggaggacaa ctttttggga aaaatccagg
     3061 aggagaattc cgacctaacg ggttccatga tggactagac tttggttcag tcgatcatcc
     3121 aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg tacgttggca atcctggtat
     3181 ttcaggtttg ggtgcgtgtg tgatcgtgat taattacgat ggcttgaata tggtgtatca
     3241 agaatttgca aatagtacag gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg
     3301 tcaagtaatt gggatacgag atacagcaca cttgcattta ggatttactc gaatggattg
     3361 gcgtcaagca caaggacatg cattcattga tgatggtaca tggatcgatc cgttaccatt
     3421 tttgaatagc agcaaaaaat agatcaggag aaaaaatatg gaaaataaaa ccttaaaaat
     3481 cgtcttaaca gctgcaatta gcgttagttt aatcggcaat attgcgttgt tttggcagct
     3541 taaagaagaa caagagacaa atacctataa aattgaagaa gtggctaaaa acgcgtctga
     3601 taagctcaaa aaaaattctt ctgaagcaca ggcgaagatt gacaagttaa atcaaaccat
     3661 caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga
     3721 ggaagtaact gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa
     3781 atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat
     3841 cgacaaatta agtcctgtag acgatgatca ccaacatgat gattacggaa gttactcttt
     3901 tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg aacaaaattt
     3961 tgtcgtattt gttgattata caatcggcaa tcctcaattc aaagatgtca aaccacaaaa
     4021 aataaaaggt ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcacaga
     4081 ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gcaagacaac
     4141 gttttaaaaa acgaaaaagt tatcttagat ggctggggat ctttatgatt ctcggctttt
     4201 tgattcttgc tagtccgatt tacacacata aaaaggtaga cataccagat gaagtaagca
     4261 cgaacgcatt tttaaacacg aaggaactga cgatggtttc aaaagaaaaa ggagcgaacc
     4321 agcaattagt ttttgaattt gcgatcgacg atgaagatca gagcttatta aaagaactag
     4381 ctaatcttaa ttatcgtgta gaagtaaaga cagctaaagg ggattatcaa gcaataaaag
     4441 ccaaagtgat caaggtgtct gatgattatt ttgtagtgaa gttagcacat gtaccagaag
     4501 aatatattgc tatgcgatta actattattc ctgaaaaaat tgatccaaag gtggatatgc
     4561 aagaaccgca agatctgatt ttttatattc acgaagataa ggtcaaagat cacgtgaaag
     4621 acgaagatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa
     4681 aagcagcaca aaaacaaatt gaatccttcc aagcaaccat tgatttgaat gaagaattag
     4741 tgaagaaact aaaagatcaa ttgccttatc aagtcgcaga agatcaggaa aatactgaaa
     4801 ataagattaa tagttaccag caagaaattg aaacatcgaa aacgcaaatg aaagatcaag
     4861 aagagagcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aatatttaga
     4921 agaataggtg gagtaaaaga aatgaagcga gcgaacttgg tcggcaaaga tactttcgct
     4981 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcaa
     5041 gaagtgaatt atctaacaca acaatatctg agttgaagaa tggtagaaag agtttagata
     5101 ccttaaaatt cagatcattt gaagctctat atatagctgc taaaaaatat tttacagctg
     5161 ataataaata aaaaattaag gtgggttcta tgtgaaaaaa acgtatcaaa agtttttatg
     5221 gattagctat atcgtcctat ttatcgttgg gtttctcgtc agtgtgtccg tgatcgtcgg
     5281 aacgcttttt cttttgttct ctgacgaaga tctggtactt aaggggttaa tgagtacgtt
     5341 atcgtgtgtc atgttacttt atttatgtat acagatcaac gaaattatgc cccctcattg
     5401 gcaagaaaag aagaaagaat ttaaggaaga aatcgaaata aaatccaatg actggattga
     5461 atacacatgg cttcctctga aaagaaatag caaacgaaag gaattttggg cattgcaatt
     5521 atggccaata ggaaaacttt ttatttatat ggcttcactt atggttactt taatcggtta
     5581 tttggtggtg gtctatgggg tggcctatta cttgttaagt gagccaaaac agatcgcggc
     5641 gtcaaatatt ttcacggcag taatgacatt aatgtgtgtc atgtgctttt ctatttttag
     5701 ttacttactc gtccaattta tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt
     5761 attggactta cttatcttag gcagtatgtt aacgactcta acagatcggt tcgaacaatc
     5821 tgaaaaaacg gtagagggga taaagaactt cttaacgacg attgatcaat ttataaatgg
     5881 atatatgtat ttgtttgaag taggctttat tctcactttg tttggttgtg tatttataaa
     5941 actcattaaa agatcaatcg aaaaagaaat ggttattgat ctaattccga cgctgagcgg
     6001 ctatgagtat tacgttgatt ttggaaagta tgaggagttg atccatacgg atatcgttca
     6061 cacgagtatc catgaagtaa ccgttaaggg catcagatct actttaaaag atggacagga
     6121 gacgaaaaca atcgaccgaa gaaaagaggt ctacaatttt tataaagttg aagcaagtct
     6181 acgatatggc attgggaaat tgaggttaga tgggagtaat attcaacaaa acaaaccata
     6241 tgttttagga aaagtggtga tttagaactt cctatgaaag atgaataaat aaggagaatc
     6301 ttttgaaaat gattcttctt tacagaattg tgcttatagt tggcacggag ttaaaaatct
     6361 taaagaagga gcagttattc aaagtgaaag aacatcataa tcagttacta tgtattaaaa
     6421 cgggtacgat gaacattcaa taccgaacag aatatttaga attaacgaat caaactgatt
     6481 ttttgtcgat tcgagagcaa cataagcaag aaaaattaat ggctacttat agccaattga
     6541 ttgatgagtg ggaacaattc aaggaatcta aaacataaaa ttccatttaa acaccttatt
     6601 ttttaaagag cataaggaaa cgtgggaaca ctttttaaaa aaagtgttcc aataggctgt
     6661 gcctattacg tttctctaaa acaaaagtgg gtacaaaata ttcgatttat ggatagtgta
     6721 tggatagtaa aacagctata catggatagc atatggatag actccataga ctatccatac
     6781 ttttttcgga gggtgaaaat ggaaattaaa gagattaaag taagaggtat taataaaaaa
     6841 tatgttcaag aaattgatca tagatgcgta gaattaacag aacaaactgg tcaaaaatgg
     6901 agtcgaaatg attatctaaa actattgatt gaaaacgact ttgaacgtcc tttaatggac
     6961 tacaagaaag atcaatttga tcgtttattg gaaaaattta cagatgttca gctgcataat
     7021 acaaaagttt tggaagcata cacgaacgag gtcaagaact taattgaaat attaatcgca
     7081 cattaggaag gagataatgt tctatggtta aagcgataga aaagcatatt acattttatg
     7141 aagcgcacga tgaagtttta aaacatatta tggaaactca gcctacggta aaaaactatt
     7201 ctgaagccat tcgattttta tgtatgaatt acgatccaga aattgaaaaa aataataata
     7261 tgacgaacgc aaagttgaac gcaatgggaa aggaaatttc tattttaata gaaatggttt
     7321 cagagtttgc ggatcttcat ttgaaagata caggaatatt aattggtaca aattcaactg
     7381 tctatcaaga agcaaaagag cgtgtggaag ggaaaataaa agcgaatcaa acgaagaagt
     7441 attctcctaa aatcaagaaa tcaataaatg aatctgttca tgccagagta gacccgtttg
     7501 atccaacaaa gtttttaaaa agataaaaag tagaagggga atatgatgaa taaatcgcct
     7561 gcagtcattc tcacttgtca atttaccttg cccaataaaa aatctttttc aacatacatt
     7621 gattatatga caagagaaaa agcgctggaa gaaatggaac gacgtacgcc tgaagaagaa
     7681 caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga aggagaaact
     7741 ttttcggaga gtaagacgga taaagaattc agcgaaacgt cgaatgaagc tcaatctttg
     7801 atgggaaatg gaatcaactt tgacaagtta gaggaacaag attttaccaa atatctttct
     7861 tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag tgaacaaaaa
     7921 gaataccaac gcttgaacca agcaataaaa aaatatcaaa cagagccaca caaaaaagct
     7981 tcaggaaacc ttcctggagt tttttctatg gcttcagacg aagtgaaagg aagtgatctg
     8041 aaggagatta agcaaatctt ccaaagagga caacaaaaag gctcaatcat ttatcaagat
     8101 gttgtctctc atgataattc gtatcttgaa aaaataggat tgtacgatcc caaaacagat
     8161 actttagatg aagaaggatt aaaagcagct ggaagaaaaa tgatggagac attattcgag
     8221 aaagagcaac tgaacgaaac aggctactgg atggcaacga ttcaccgtaa taccaaacat
     8281 atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaacctta cacggaggtt
     8341 gaaaatggtg tgagttatat cgtaccgaaa ggtaagagaa gacaagcaac attagacgca
     8401 atgaagacca attatgtaca tgagttagaa cgtttttctt atgaaaagga tcgaatgatt
     8461 ggtatggaga aacgaaattt attgacgcgg aaaagtgatt tgcgtaacac cttaacaaaa
     8521 gtggtaaaag agccagcgcg ttacgatcga cacgcaatga acttattaaa tgaagtatat
     8581 aaaaccctac ctgacaaacg ttcggaatgg cattatgggg atgaaaaacg aaccaaactt
     8641 tcaccataca caagagagaa actcgatgag ttaaccaaat atgttttaac aaaagaacct
     8701 gaatatcaag aatatataca gatttcaaaa gcattgaaag aagaagcaaa aagtctttat
     8761 ggggaaagca aaagagaatc aaaggatgca gaaaaaaatg ctctctttga tttgaaaaag
     8821 cgcacaggaa atgccattct aacagaactg aagaaacaga atagtcaaat aaaaccttta
     8881 atagaaagta tggatcagat gcaatctatt tacaatgaac gatcctttcg aacgcttaaa
     8941 cagttaccaa cgaatgaata caaatatagt tacgaagaat tcatgaagcg caggaaacaa
     9001 ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat taaatcgaac gattaatgcc
     9061 catgaagaaa aataccacgc aagtaaagcc tacgaagagc aacaacgaag aatccaaata
     9121 gaacaagaaa gacagtaaag acagtaaagt tagcgcctta ttatactact tgggaaatga
     9181 atgaagaggg gatcaataac ttcccgtgta aaagcattga agagtctatt tcactcacag
     9241 aatgttagtt gttaagtgaa taaaagaagt aaaagagtcc tgaacgtaat tgtccaggac
     9301 tctttttttc aatgaattgt tttccgccaa ttcaatgagt ggtgcgattt gcgactacat
     9361 tatatcaaaa gaaagagaag ggaggaagaa gaaatggctt attcaacgat tggagttgtt
     9421 gttgaaaaaa gcagagacaa tctcgttttt gtgacagaaa ttcaaacagg cagagctttt
     9481 gtcgtcacag acaaagcggc aaaagcgtat caaaatggcg atattttaac gttaaatctg
     9541 actacgaaga cgttcgtcga tgc
//
LOCUS       DAFBQW010000090         9494 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFBQW010000090 DAFBQW010000000
VERSION     DAFBQW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.093"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1655)
                     /locus_tag="LK889_002616"
     CDS             complement(<1..1655)
                     /locus_tag="LK889_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5096811.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQVGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAV"
     gene            complement(1707..2378)
                     /locus_tag="LK889_002617"
     CDS             complement(1707..2378)
                     /locus_tag="LK889_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK5096812.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFRLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(2464..2658)
                     /locus_tag="LK889_002618"
     CDS             complement(2464..2658)
                     /locus_tag="LK889_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096813.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            complement(2770..3009)
                     /locus_tag="LK889_002619"
     CDS             complement(2770..3009)
                     /locus_tag="LK889_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096814.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(3123..3464)
                     /locus_tag="LK889_002620"
     CDS             complement(3123..3464)
                     /locus_tag="LK889_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK5096815.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(4083..4415)
                     /locus_tag="LK889_002621"
     CDS             complement(4083..4415)
                     /locus_tag="LK889_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK5096816.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(5262..6302)
                     /locus_tag="LK889_002622"
     CDS             complement(5262..6302)
                     /locus_tag="LK889_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5096817.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            7468..7554
                     /locus_tag="LK889_002623"
                     /pseudo
     CDS             7468..7554
                     /locus_tag="LK889_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8114..8332
                     /locus_tag="LK889_002624"
                     /pseudo
     CDS             8114..8332
                     /locus_tag="LK889_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8592..8846
                     /locus_tag="LK889_002625"
     CDS             8592..8846
                     /locus_tag="LK889_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5096818.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLIILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            8847..9164
                     /locus_tag="LK889_002626"
     CDS             8847..9164
                     /locus_tag="LK889_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5096819.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT         2963 a         1731 c         1418 g         3382 t
ORIGIN      
        1 actgcttctt tgacaccatc tttatctgta tcagtaagtc caccgtagta cttaccttct
       61 gcattcttag ctgcaaattc tgcgccttca agaggagttg ccttaattcc tgttccaaat
      121 agtccgctat cgacttttaa gaatttcgcg ccataagtca caacgtcagc tttcgttttt
      181 tcgtggtgtt cttcctgatt aaatggattg ttgttataaa cgaaatccac gctatttgtt
      241 aatggattgg caacagctgc gctgtcattt acatacatgt tataagtcat ttgaagatct
      301 tgtccgtgta aacttgctac agtatcactt acttggccat tatttacgat aaaatcgatc
      361 acaaagccat tttctgaatt ttggatctta taatctgtat tttcttttaa taccactgat
      421 ccatctttat ttgttacttt gatatttgct ttagcagcga cataagaaag tccttgtcct
      481 gctgtatccc cccatttaaa tacactgtaa ggagaagttt cgcttttcac gccgtttact
      541 gtgataccgt ctgctccgcc tgcaatgtct gttgggatat gaatcgttcc ttgataatta
      601 acggcatcac caacttggaa agaatgttct tgtcctgttc catttgttac tgttttatcg
      661 atcactggtg ctttatagtt tacgtatgaa gcttttaagt cacttgtcac gccatcattc
      721 gtaatttcaa aggttaattt attgtgcgca tcgttgcgag cgtctgcgcc taataaatat
      781 tgatcggctg ttggttcttt atctgatccc actacgactt ctgaaggaac ttccacaaaa
      841 tagtattttc ctaacgtcaa atcagtagca gtcagctttc cttgatcatc cgaaacaaga
      901 tcgcctagtt tgcttccttt tccaggttct cctttataca attcgaattt agcgccttta
      961 agaggggttt gtttgctttg ctctgtacca tcatcaccga atttggttaa ttcaaaactc
     1021 aacttagaaa caatattttt aggatataag tgaatgtctt ttaaaaaacc tgaacctgtg
     1081 ttgtcagtca ttggagcaat gaccaccatt ggttttgctt tttgagttac taatcctgct
     1141 gcggacttac tctcgtaaat aacatacaca tttccttttg agttcacgta cgctggttga
     1201 tccgcaaaag taatctcacc attttcatct acagctgaag taattttatt ggttgcattc
     1261 ttgacgtact cactgttaga tcccttgtct tctacgtctt tcacgatttc gtccactttc
     1321 gcgttggtta gatcagaatc ttcagtcggt aatacttgat ccgtaatatt tcctaacgta
     1381 aactcaacat caccataatc tgccttgttg tatttcgtta cacctgtagg atactgatcg
     1441 tgcgtataac catcattttt gatcccgtca atatcaacat tcaattgcgt actttgatcg
     1501 tacattaatt tatgaaccgt gatattgacc ttctctggtg gcgttttttg cgtgacatct
     1561 tctgcatgtg cttgataccc ctgaaagcag cttgcaatga ttcctaacat tccaacaagc
     1621 attccagctt tttttacata tcgatttatt gccattttta ctctcctcat ttcatatttc
     1681 aacgactcgc gttgattaaa accttgttat tgtagctctc cctgtaccac gtatctttct
     1741 tttccttgtg acgtatagac acaagtaaac aacgttaata agggtttctc tcctttcttt
     1801 ggcaaagaca agaaagagtt ctcttcagga acgccatcga ttaatctaaa gtacccaggt
     1861 acgataaatt tacgcgttac cttgtaacga tagacgccat cgttactaac taagtccatc
     1921 acctccccta atgacaactt ttgaatatca gaaaataaaa cacctggcat ttccatgtta
     1981 tgacctgcta ggacatagtt tcctttgccc atttcagcat cttcataaaa ataagtagcc
     2041 gctccaagac tcaaagtgta ttgatttgcc cctttaaaaa ttggcaatgc taaaccaagt
     2101 gcaggaatct gaatttgtgc aaatacttgt ttattgacgc ccatttcgat tgcttcttgt
     2161 tgtatggaca acatttgccc caaagacata tcattattga cgttcgttgc atcctcctta
     2221 taactctttg catgtgcatt gatttgatct acgggcatct ttttttctat attttgctta
     2281 aacacctctc cttcatgttt ttggtagcta acatagccaa acacactgca aaagacgatt
     2341 aaagcgactc ctaaaaaacc aaataaccat tttctcatcc agctaccccc tttcagacaa
     2401 aacaaaaaat ctttttctca tttccataga aaaagtttta aaaatgaatt ctattaatga
     2461 atgtcatgag acttctaaca atagttgaca attagaactt gttttcacat aagttcgtct
     2521 caactgttca aagtttttca ttgaacctaa caaacaagct ttctcttttc ctgttatctg
     2581 catatcattg atatattgca tgacagataa tcttttaagg tgtttttgtt tcatcgtttt
     2641 tagcaacttt tcttccattt atttcgacac tttttttctg ttttcgattt cttcataatt
     2701 catgagtttt cctaccttcc ttgttttttg caaaaataaa aaagcctatg atcacatacg
     2761 ctctacactt cagtcttggg ttatcttttt ctttcgttta attactggcc gtgctggttt
     2821 caaatccttg attccattat gggttctcca ataatcgaat tcttttcgta atctttcttt
     2881 ttccatatct gttcctttag acagggattt gaatacatct ttaggtgaat aaccacgagc
     2941 taacagctgt acataccttc cttttgtcaa agtatccata tccatcgcct ctaaaactgg
     3001 tcgcgccatc taattttcct cctaaatgaa ttccttactc aatgcctaag gtaatactac
     3061 ctttttatta ttcgtcgtga cttcttctaa gcctttatct gtaaaattta ttcctccagt
     3121 ttttattctt ctttatccgc tacaaattca atgatttcaa cgctgacaat ataatcactc
     3181 aaatatgacc aagtatcttc aacagataat tcgtctccat tatcattcgt aagcgtcatt
     3241 ttaaaccatt cattaccttc atcatcttca ggtagcaagc cgaatccaat tggattatca
     3301 gtcaattttt gatagtataa atcatcctcg tctaatgcta aaacatctaa tcctttacaa
     3361 tttccgcctc ttctaaacgt cacaaatcct ttgtaatccc aacgttggaa agaaacctta
     3421 atagtatgtg tgcccaatcg tatattcgga tcataatttt tcatttgtca ttcctccatt
     3481 acgtaaattt cattttctct atgttttata tcaatttttt cactttattc aggactcgtt
     3541 catattttat cgtttttcgt ttacttaaaa atacataaaa aatatcgact cgtttatggc
     3601 gtccgaaacg agaaacccta gtagaaatcg gtaaatgctc gattgatcca atgaccaacg
     3661 tcctcgcaag tatctacatg tactgcagca cacgacacaa tttcttgtgc gtcctcccca
     3721 ttacgagaag acctactcct tattccatga gtttgcgtca aagcctagat cccttagtag
     3781 ctgctcatga ttaatctagg cgctattcta acttgtctaa cttctaaatt ccctcttgtt
     3841 tactctgtgc atattattta cacaaattca tttcaccact atccaaacaa gggcatttac
     3901 tcgattgacc aattttttac gttaccaatc gtaaaaacct accccgtcac cccccgaaag
     3961 gaattgttga tagatcaagt ctactcctta tttttctagt aattctaagt tcttttagaa
     4021 gtctcgacaa tattttcaag caagcgaaaa acgataaaat acaagttatg agaatgatca
     4081 attcagctga ttaaacgccc catccctttt gcggtacaac gcaagactcc attttttctt
     4141 cgaccaatca gtacaccgtt ttggtcataa acatgtttga ttgttggata tagttctttt
     4201 aaagggatag gatcttttaa aattatttgt ttttgtttac cagcgtaata aatcatttcc
     4261 tctgagactt tgtctttctg gattatatat ttcttacact gcacaacggt tttactttgg
     4321 ccagttgata gtttcttcgt gattttctct gtagcggtac accgtttacc gtagtgacaa
     4381 atatcttcga gactcaaaat tcctgtatat gacataaaat aatccaccca cttactttaa
     4441 atatttttaa taagtttttc ttgttatttt tcaaaaatag agaattgatt ttcgttcatg
     4501 acaaacaaaa aagagggaac gcatccaaac atgcgttccc tctaatttca gcgttcgtca
     4561 tacggcaaag agccacacgg acatttttat aacattatgc ttaggcatag attcatcgta
     4621 atatcacgta tttttaacct taacacagcc aaaaaaagtg gtatcacttt tgaatatctg
     4681 ttatactaaa tccgttgcgg gtaataccgt aacactgaaa ggtaaacgca ttaagggatt
     4741 aaaggttgtc ttaatgccac ctgtggacgt cgacgaaggt taccagcctt ccgagtctca
     4801 cagggccttt cttttttttg tattttcaat tgtccaaatt tatcgtccaa aagtgcagaa
     4861 cgaaaattaa ttcatcacaa gcgtagcaga caattaaatt taaaacaact aaatttatac
     4921 aaaaaaatgt aagcgaataa tattcgtatt gcctgaaaag aagtaatatc aaggttttta
     4981 gcttttttaa aaaatgaaat tacctctctt cgcgtattat tataactaaa aaacagcaac
     5041 atgcaaacgt ttgttctctt ttttgtgttg gaaattaatt attaactatt aacacttatg
     5101 acgtaaaaag ctacgtttgt gatttttatc ataatatttt aactcataaa ttctattaaa
     5161 atagctattt ctttgtaaca tttgtaatat aattagaata ataaaaaatt tttaatttta
     5221 atgctactcc atgaagcaac aatcaatttg tcagtgaaat ttcactctaa ccagtctttc
     5281 gtaatagttt ttgggaagct ttcagaattc aaacgttttt cacttgatac tttagcagct
     5341 attggttttt cgataaacca attttttaca tagacaaaaa tcaggttctg gatattttca
     5401 actttttcgg tttttaattt ttgataggct tttaacagtg tcgtgtataa ttcttgttct
     5461 gcattgacat aataactatc gagttcttca aaaattattg aaataccact ttctgtttcg
     5521 gcttttttct ttgcattatg aatagtctgg acagtttttt gagcttcact gtaacttgaa
     5581 gaaaatgttt ttattaaatt taatactcgt tcagggataa acgtagcaat gctgtcgttt
     5641 gtcataattg tctcaaaatt gtctaacaac aattgatctt gtaggcggtc tttttcagtg
     5701 tctatattgt gtctattagt gtctaatata ttattattaa gttctggtat gattttcata
     5761 ccctctgatt tatcaacgtt ttggcacggt atgattttca taccctcttg agccccaagg
     5821 gattcagcgt ctgatttttc gtcgagggtg ggtatgattt tcataccctc tgatttatca
     5881 acgttttccc tattctctct atcaaatttt tcgagtaagt agatatcatt ttctgagact
     5941 tttaattctg ctagatatag tctattagga ttatttttcc ctaactgttt gttaaatccc
     6001 atctgctttt gtaataataa accaaaggat tccaactctt ttttgattgc tatcgctttt
     6061 tgtttttggc agtttaatac gctacatagt tcatttgtcg tataagtaaa ataaacatta
     6121 ccttcttcat cgatttgccg tttgctgatt gcgatctcta atcttgcttt taaaagcata
     6181 taggcaatct tagttgaatc tttcattttt ttgtattcgt cagaaacaat aaagactttt
     6241 ggaaattgat aaaataattc gttgtaaact ctatccgcct taaaaaaatt aaaatcagtc
     6301 attgtagatc ctctactttc acattgtttt atttatgtaa aattacctac tcttttcgaa
     6361 aatagcaaag ataaataaaa aatgcaaacg cattaaaact gttttttaat taaatactac
     6421 ctaaaatcag aaataaaatg aattatgtac tacctttaaa gtcattaaga actactttaa
     6481 ataaatatta tttataatta aatactacca gaagaacgta tgaataaaat ttagttttaa
     6541 aattaaagat aattaagtac taccagtgtc aaaacaaaaa ataaattaag aactacctta
     6601 tgcatataaa acatactacc attactagta atatatacta ccttatttca gtagtatata
     6661 ttatattctt cttataagac aaggctttag gctagaaaac aatagattat atactactaa
     6721 aaaatacaat aagtactacc aatgtcagat atatataata attatgtact accattaaaa
     6781 ttaacttatt taataagata ctactgtgtt aatctgaaaa aaattacttt aaagtagtta
     6841 catactacct gttaaattag atactaccat taatacttaa attagatact acctatattt
     6901 atattaagta ctaccgttct aggaataaga taataaaaaa aaactaccaa attgcaatta
     6961 aatactactt aaaatgtaaa aaataagaac tacccaaatt aggtagttct taataagaga
     7021 atatcagata aatttgtaca agtcataaaa aaattaatct taaattttta aatatatata
     7081 cctgttaatt ataaactact ttatttaggt agtacataat taacagataa aactacatgc
     7141 ttgtagtttg tctagctaat gagttgcata tgatttatat aaaggtgtgc agcataactt
     7201 agttgaagtt aagagaatag aataaatatt ttttgttttg ctaataatta aacactacct
     7261 aaatatggta gtgtttaatt atataatatt cattttgtta caaataatcg tattataaaa
     7321 gcataacaat tgggataact attttttaga ttatttaagt actacttaaa ttaggtagta
     7381 cttaaatttt ggtttaaggt agtaggtaat taagaaataa tatatatctt cttttataat
     7441 agaaggggag aggcctagtt aaaagcagcg acaattaata acaatgtatt taatgcttca
     7501 caaaatccat ttggtggaca atgcattgtt attagcgatg atctaccgtt ctaagtagtt
     7561 ttttctattg gttgcattcg tgactaatta tggtataatt atgagtaaca aagaggtatt
     7621 tcattatatc atcaaacccc atcagcgtgt aggctgatgg ggtttttttt acgtttctat
     7681 attatagaat taactggtag gcttactgtc ttttttggca attttgcaat caagccactt
     7741 gcagatgtag tgtgcagtta cctctgcaaa tggcttgatt gcaaaaacaa aatcaaaaag
     7801 gtatttcatt atatctcctc ctccccttgc cagattaggc cggggtagca gacagcaaaa
     7861 ctattataac ataactgaaa tacctaaatg acatatacag atatgataat ttatgtattg
     7921 atttttctaa caaaagcaca tattttaata tatactacct attttaggta gtatatattt
     7981 ttagatacaa tatcgatcag ttttgaggaa atgaaattct agatattttt tgctacatag
     8041 agttactttc aatatttttt aacgtaatta ggcagaagtc actcatcctc tataggtttg
     8101 tgatgaaatt gctattgatc catttaagta agagcaaaaa actggaggtt tatcaggtaa
     8161 ttggtctaat ggatttaagt atgcggtcac cgtgtataga gttgcttatg agattcaaga
     8221 aaataaagtt attccagtat tgctatatgg aacacataac aatttttatg aattagccga
     8281 gaacactcgc aactttattc gtgtagatga atcggcttcg gttgagttat aactttagct
     8341 atatcaatca accgaccgac atatgttaaa tctatatata ataaaaatac ctacttaaat
     8401 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
     8461 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
     8521 aaacaccgtt ggcaattgtc gttataacat gttataattt atttatcaaa aactaaaaga
     8581 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
     8641 tccttccaag ctctcttgga ttaaaaagcg gagacactat ccaagccaaa caagaaggca
     8701 acttaatcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
     8761 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
     8821 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
     8881 tccagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
     8941 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
     9001 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
     9061 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
     9121 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggcaaaa
     9181 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
     9241 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
     9301 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
     9361 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     9421 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     9481 ttgacgtaac tttt
//
LOCUS       DAFBQW010000091         9444 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFBQW010000091 DAFBQW010000000
VERSION     DAFBQW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.094"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(130..972)
                     /locus_tag="LK889_002627"
     CDS             complement(130..972)
                     /locus_tag="LK889_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5096820.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1009..2475)
                     /locus_tag="LK889_002628"
     CDS             complement(1009..2475)
                     /locus_tag="LK889_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5096821.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2531..4375)
                     /locus_tag="LK889_002629"
     CDS             complement(2531..4375)
                     /locus_tag="LK889_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5096822.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4977..5927
                     /locus_tag="LK889_002630"
     CDS             4977..5927
                     /locus_tag="LK889_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5096823.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(5972..6605)
                     /locus_tag="LK889_002631"
                     /pseudo
     CDS             complement(5972..6605)
                     /locus_tag="LK889_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6865..7413)
                     /locus_tag="LK889_002632"
     CDS             complement(6865..7413)
                     /locus_tag="LK889_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096824.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7414..8271)
                     /locus_tag="LK889_002633"
     CDS             complement(7414..8271)
                     /locus_tag="LK889_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5096825.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(8557..8844)
                     /locus_tag="LK889_002634"
     CDS             complement(8557..8844)
                     /locus_tag="LK889_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5096826.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(8834..9163)
                     /locus_tag="LK889_002635"
     CDS             complement(8834..9163)
                     /locus_tag="LK889_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5096827.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(9240..9386)
                     /locus_tag="LK889_002636"
                     /pseudo
     CDS             complement(9240..9386)
                     /locus_tag="LK889_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         2848 a         1906 c         1444 g         3246 t
ORIGIN      
        1 gattttttat tatcttacgt taatcctttt taaatgtctc ttatagataa caaacagctg
       61 gaaatcacct tgtttttact ataatccatc tctaaagatt ttaaaagcat caatagtatt
      121 actgagcgct cacccattag catgtttcat caaccgattg atgtggacac ctaggtatac
      181 aacttcatcg ttcggaattg ttttatgata aactttttca atgtagtctt tgatcttagc
      241 tgcgctttcc atggcagacg ggtataagat ccacatttga ttgtagaatt cttcctcacc
      301 atcatccaac aaagcatcag aataatatct ttctacaaag aaacgtacat gtgtaatgaa
      361 gcgggcataa tggatcgaat tagtatcgat atctttctgc aacgaatacc ggaccaaatt
      421 gacgatcccg ccgatcattt ttgcatactt gaatccagcg ccgctatcag gatcttccga
      481 ttgtgcattg atcaaatgga aagcaatgtt tgaggcttcc tctttaggaa gatcaattcc
      541 atattgctcg ttcaaaaggg tgagtgcgta cttcccaatt tgaaattctt tgggatagta
      601 gatttttatt tcccagtaca aacggtttgc aatatttacc cctttttgtg cgcgttcaac
      661 tgcaaaatgc aggtggtctg ttaaggttaa atagactcca ggattcagtt ttttatctaa
      721 tcctttttct gcttcaaaaa cgatagtacg agtgatttca aaaaattcaa taggtatttc
      781 ttcaactaac tctaatagtt gtgaagattt cttttcttca aaaggcagaa atattttatc
      841 tactttatct cctgatattt catccccagc tttctgtcca aagcctatcc ctttacctaa
      901 tgcaatcatt tctttgccat tttcttcaac aagtaccaca cttgaattca atactttttt
      961 tattgaaatc actcattatc cccctatcgc tgcttaaaaa gatcgagact actcgaataa
     1021 ggatgcaccg tttgtttcga tcacttcttt gtaccagttg aacgaatcct ttttcaagcg
     1081 gttatatgtc ccatttcctt catcgtctgc atccacataa ataaatccat atcgtttaga
     1141 catttgagaa gttcctgcac taactaaatc aattggtccc cagctagtat aagctatcaa
     1201 atctacacct tcatcgattg cttctttcat ttgttggaag tgtgctttaa agtagtcgat
     1261 gcggtacgga tcatggattt taccatctac tacctgatcg actgctccca taccattttc
     1321 cacaatcatc aacggtactt ggtaacgatc ataaagttcg atcaacgaaa ttttcaaacc
     1381 ttttggatcg atttgccagc cccaatctgt tgaaggcaaa tgaggattct taacccctaa
     1441 aacagtattt cccggtgtgc gttctgcgtt tggatcagct gattctgcca tggacatgta
     1501 atagctgaaa gaaacaaaat ctacggtatg tttctttaaa atttctcgat cttcatcttc
     1561 cattttaatc tgaattcccc gattttccaa atccttttcg attagagcag gatattctcc
     1621 cttaacttga acatctgcat aaaaattatt ttctaagttc tttttcaagg tcagctcaac
     1681 gtcttctggt gcgcaagtta atggataagt catcaatttc gttaacatac aaccaacttg
     1741 gcttcctgga attttttcat gagcatcttt tgtcactaac gcactcgcaa caaattgatg
     1801 atgcaatgct tgatagatat cttgagcttc ttgaccttct gcgcttttct cttggataat
     1861 tccagctgtt gtaaatggat gacggtggat gctgtctatc tcattaaatg tcagccaata
     1921 ttttacgtat ttgccgaagc gatcaaaaca aacgttagcg aagcgaacaa aatcatcaat
     1981 tactcgacgc tccacccagc cattatattt aactgctaaa gctaatggca tttcataatg
     2041 agatagtgtc acaatcggct caattcctaa acgttgcatt tctttaaaga cgttttcata
     2101 aaatgcaacc cctttttcat tcggttccag ctcttctcct gtagggaaca agcgagtcca
     2161 agcaatagag aggcgcaagg ttttaaagcc catttcggca aataaagcta aatcttcttt
     2221 atagtgatga taaaaatcaa cgccccttct ttttgggtac catttatcag atttgtcttc
     2281 actagcttct tttatagact gcagggtgac atgcatatga gcagcaaaat ctttgttgtc
     2341 taaatttggt ttataagtag ccatatccgc tactgaaaga cctttaccgt ctacattcca
     2401 agcaccttct acttgatttg ctgctatcgc gccgccccat aaaaatcctt ttggaaaact
     2461 tgttgtgttt gtcattattc tattctcctc tattccttca ttcaatatat tttttttcac
     2521 tatcttgtgt ttaagctatc ggactgatag ctaagagatc actatcattg gttaccaatt
     2581 tttctaggtt aaatgaacgg atagtgtaat tatccttatt ggttaccaca cagataactg
     2641 tggggtcgaa tcctgcttct ttgattaaat caatatcaaa tgcaatcagc ttttgtcctt
     2701 tggctacttt ttcaccagaa cgaacaaatg cctcaaaccc ttttccttct aactgaacag
     2761 tatcaatacc aatatgaaaa agaagttctg caccattagc cgtttttaaa ccaatagcat
     2821 gcttcgtttc aaataccatt gtgatttctc catcttcagg agctactaat atcccttctt
     2881 caggaatgat tgctactcca tcacctacca taccactaga aaaaacagtg tcttttactt
     2941 ctgatagtga gattactttt ccattcacgg gacttgaaag aacagcatct gaaacaacgg
     3001 ccgtttccat cttagtttca acttcattga caacttcttt atctttataa agaaccaacg
     3061 ctgcagcaaa agcaaccaca aatgaaatcc ccgatactgc aaatgcccaa accagattca
     3121 ttgggtcagc tttgtcagcg aacatcgtga tacttgcaat cccaggaccc actaaagcaa
     3181 aggcttgaag tgcaacgatt cctgcaaatg ctcccccgat caggctagaa aacataacgg
     3241 catataaaac acgtttgtgc agaatcgtta ccccgtaaag agctggttca gtaattccga
     3301 ataatgcaga tatacccgca gaaacagccg tagatttcaa ttttttgtct tttgttttga
     3361 tactgacagc aaagcatgcg ccagcttctg aaatattgtg tgccaatgaa gccgggagat
     3421 aaagaagttc tgcacccaat tcactcatgg atgaaacagc gtaagggatc attgctttat
     3481 gcatacccgt tacaaccatt aatggcagta cagctgctaa gactccggtt gcaacccatc
     3541 caaattgatt gtacaaagac acaatgatag aagagaaagc tgttcctaca ttataaccta
     3601 atggtcctag aagcaacaaa gcaataggaa ctgtaatcaa taagctcatc ataggtacaa
     3661 agaaaatacg aattggtttt ggtgaaactt ttgtaaacca tttttcaagt tgagcataaa
     3721 ataaaacagt taagatggct gggaatactt gagatgcata cgctatatta tccaatccaa
     3781 aagataagaa tgtagcacct tccgccaaca tagctgtcat ttcaggaagg atcaatgcac
     3841 cgacagcaga tactgcaacc aattgattga ctttcaattt attagccgta gtcattgcca
     3901 ccagaatagg caagaaataa agcggtgccc cgccgaccaa atttaaaatc tgataggttt
     3961 gagattggtc actaatcagc cctaagatag aagctagtag caacaaggac ttcaatactc
     4021 ctccacctgc aatcgcagga attaaaggtt ggaaaataga aataataaaa tctaatagaa
     4081 ctgaacctaa tttttgtttt tgttttggtg cttcttcagt atttgaaaca ttgtcaccta
     4141 ataacgcttt cacttcatca tacacttcaa caacatcgtt tccaatgatt acttggcatt
     4201 gcacattttg gcgaacgccc ataacgccat ccactttttc taactggctc atatttacct
     4261 tgctgttgtc ttttaacgta aaacgtaaac gagttgagca atgctccaaa tgggatatgt
     4321 tttcttcccc accgatgtac tgcaatactt cttctgctgt ttgtttgtaa ttcatggtgt
     4381 ttccctcttt tcttttttga gtgatgaact tttgagtcga agtggccatc ctcaacacaa
     4441 aaaacaggca gaaactaaat aaattgacaa caaggcaaac gtgtattgaa acgcatacat
     4501 tgttattcaa cttatttagg tcctgcctgc attaccagtc acatgcctgt ttaagttata
     4561 ttcaatttaa catattagaa tgtaaacgta aataataaaa tgatttttta atcatttttt
     4621 cattttattc gttcaatata gttttcgtgt tctaaaagtt ttgtcttttt tctaagaata
     4681 tcttccaaca tatataactc ttattctctt ctatcttcta gcagatgttc tgtcttttta
     4741 agacctagta taaaaagaat gggttcattt tttctttctt gcaggatgag gttttttata
     4801 taaactttat ttgatataaa gaaaaggcat tcataattgt gctatactac taaaaaatga
     4861 taatacagtg aattagatta gcgctttatt aaattaagag gacagcatat cgatattgct
     4921 tccttttttt tgctatttat ttaagggata gaagtttaga aaggactgtt tataagatga
     4981 aaaatgatat agtgacttat gcaaacacaa aatgtgaaga aattggcatc agtaatttag
     5041 aagagtgctt ttttgacaca aatgacctgt cgcaattttt aagaacggga agaacaaatg
     5101 ccagtaaact tgccaatgac ttagttgcaa agaacgttct atttaaaatc aatactcgtc
     5161 cagtgttatt tgccatcaaa gagcgctttg aagagcactt tttgattccg gtaaaagatg
     5221 tatacgaaag tatatctgaa ttaaaaacag acttggagcc caattctaca gattccattc
     5281 tacaaaagat gattgggtat gatatgagct tatccgatgc ggtggaacaa atgaaaactg
     5341 ctgtcttcta tcctggaaga gggcttccta tgatgatcac tggagatact ggagttggaa
     5401 aaagtttttt tgttcatact gcttatcagt ttatgaaaaa agtgaacgtt attcatgaga
     5461 atgctccttt taaagtactt aattgtgcgc aataccataa taatcctgaa ttattgtcgg
     5521 ggatactatt tggctatgtt aaaggtgctt ttaccggcgc agatgaagat cgaaatggat
     5581 tgttgcaaga tgcagacggt ggagtactgt ttttagacga agtacacaga ttgcctcctg
     5641 aagggcaaga gaaactattt gtttatatgg atggcggaac ttattctaga gtcggtgaaa
     5701 ctatccatca cgcctccaat gtacgtttag tttttgcgac tacagaaggc aaagaggatt
     5761 ttctggaaac ctttatgcga agaatcccta tacatatcca tatcccaaat attgaggaac
     5821 gaggtatttt agaaaaaaaa caaattatcg aaaatactct ctttagcgaa agcaaaaact
     5881 ttagcaaggc tattgatgtt tctcctaaag tcactagtgt tgcataaata tagtcagaat
     5941 attctgaatt attattttct tgaaaaaagt aaaaaaaatc gtatcccaaa ctttcatcta
     6001 catttccaga aagatcttca tgttgataat caattcctga atcaatgacc gctaccttca
     6061 catccttaga acccttggtt tcttcccaag cggatggcat gctaatctct cttaaattag
     6121 cttgtttact ccaattagga tcgttatcga tttcctaaag aatcagttgt atttggctcc
     6181 tcaacatcat tcaacggctc tagttcaacc acatagtttg gctctgcata attgaccata
     6241 tcagaagcat taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact
     6301 aataatactt gatctcgctt attataatcg ctttcatttg tcctaagagt caaatctttt
     6361 tccaccataa cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt
     6421 tgtgtagcct ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct
     6481 aattggctca taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc
     6541 atgaacccaa acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt
     6601 ttcatctaca tctcctccta atagttttat tttagagtta gctctattac ttatttacta
     6661 actcaacaac agattatcat acacttatta atgaaatcac aatatctcat taaaacacac
     6721 cctaaaatca taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa
     6781 aataaatccc ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt
     6841 agttacttag ttcttctttg attattatct aactttatta ttcattcccc accctttgcg
     6901 cagtgagtct tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc
     6961 atctaattct ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc
     7021 attaaacaaa tcttgtttat cttcttgcgt aagattctgt ttaatatact catctaaagc
     7081 ttttctaagc aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga
     7141 agtcgcattt aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa
     7201 ctttctgatt ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga
     7261 ggaagttttt atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat
     7321 tgcttgctgc tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt
     7381 cccaaaaata tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc
     7441 gtgttcttcc aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg
     7501 ttttaagaga cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa
     7561 gcgcgcattt ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg
     7621 tttatacatt tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc
     7681 aacatataaa tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat
     7741 cgcatttctc atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta
     7801 catcgcggat ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa
     7861 ctcatcaaat aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt
     7921 atattggtct ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata
     7981 gaggttattg attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc
     8041 agtcacagta ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa
     8101 gttcatatgc gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat
     8161 caaggttctt tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact
     8221 tttacctacg ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc
     8281 tcttttcttt tagcaatatt catttgatac tattataaca tatttatttt tagttactac
     8341 caaaaaaaag atatatatgt actaccttat aattaataag taaaaggtag tacataaata
     8401 aggaaaagct gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt
     8461 atcgagaagc agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa
     8521 aaagaagccg tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc
     8581 ataaaagttt tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc
     8641 gtaagctact ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa
     8701 atccccagtt ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt
     8761 caaaaatttt tctttcaatg gcttctcttt tagctttttt aaatacttaa tgacatgttt
     8821 tgaaggaacg atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag
     8881 aattagtaag tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat
     8941 tcttaaattc tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa
     9001 tatcttcgct aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat
     9061 tctctaccaa ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg
     9121 tcaacatgat tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct
     9181 aacagtatgt aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat
     9241 tactgtgact cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct
     9301 accttataaa caacccggtg ttgtctattc aatcttctaa aatacctacc ttcatgtgta
     9361 ggttttaact tctcaaatga ttgggttctg ttgcaaagtt ttaaataaag aataaaatcc
     9421 cttacggtat ctatgattta agct
//
LOCUS       DAFBQW010000092         9328 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFBQW010000092 DAFBQW010000000
VERSION     DAFBQW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.095"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            317..1102
                     /locus_tag="LK889_002637"
     CDS             317..1102
                     /locus_tag="LK889_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5096828.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1115..1966
                     /locus_tag="LK889_002638"
     CDS             1115..1966
                     /locus_tag="LK889_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBK5096829.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2090..2542)
                     /locus_tag="LK889_002639"
     CDS             complement(2090..2542)
                     /locus_tag="LK889_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096830.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3007..3375)
                     /locus_tag="LK889_002640"
     CDS             complement(3007..3375)
                     /locus_tag="LK889_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBK5096831.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3525..4007
                     /locus_tag="LK889_002641"
     CDS             3525..4007
                     /locus_tag="LK889_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBK5096832.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4044..5054
                     /locus_tag="LK889_002642"
     CDS             4044..5054
                     /locus_tag="LK889_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBK5096833.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5169..5882)
                     /locus_tag="LK889_002643"
     CDS             complement(5169..5882)
                     /locus_tag="LK889_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096834.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(6072..6662)
                     /locus_tag="LK889_002644"
     CDS             complement(6072..6662)
                     /locus_tag="LK889_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBK5096835.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7185..7856
                     /locus_tag="LK889_002645"
     CDS             7185..7856
                     /locus_tag="LK889_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK5096836.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            7853..8014
                     /locus_tag="LK889_002646"
     CDS             7853..8014
                     /locus_tag="LK889_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096837.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            8498..9268
                     /locus_tag="LK889_002647"
     CDS             8498..9268
                     /locus_tag="LK889_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBK5096838.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
BASE COUNT         3209 a         1445 c         1763 g         2911 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag
       61 cttcttcgga aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact
      121 tcttatttct tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga
      181 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
      241 ggtgtacact cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga
      301 aaaaggagtg ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg
      361 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
      421 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
      481 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
      541 acttgatacg gctttagaaa attttgggac actggacgtt tggattaata atgctgggat
      601 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt
      661 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga
      721 taaaaaagga aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt
      781 tgctcattac gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga
      841 gtacgcacca aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat
      901 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
      961 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     1021 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     1081 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     1141 ggcagttggg tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     1201 tgagatatta ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc
     1261 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg
     1321 ggtcatcgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     1381 gccaatttcg actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg
     1441 agaatggtct ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt
     1501 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     1561 aatcagtcta ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact
     1621 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     1681 agtgatttat gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc
     1741 aacaatagtt ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga
     1801 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     1861 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     1921 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     1981 aaatctcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     2041 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     2101 gtgaaatatc tgtctgcatc aataattttt cattgatcca ttgactaaag tttacgacat
     2161 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     2221 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     2281 attgattgat tgctgtaaaa tcacgatact gaaggttgaa acgcatcgtg attggtgcat
     2341 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     2401 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     2461 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     2521 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     2581 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     2641 tcaagtcaga aaattctaac ttttctttct ctaaagaaat tcgttctctc ctaaagtgat
     2701 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
     2761 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
     2821 agtggaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
     2881 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
     2941 tgaacaaaac ctgtataagc tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
     3001 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
     3061 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatctgt
     3121 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
     3181 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
     3241 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
     3301 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatctgat tgatgatcgg
     3361 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
     3421 acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg ctatatttat
     3481 atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt gacttggcaa
     3541 agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc atttatacaa
     3601 atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag accaaaagct
     3661 tattcttaaa agataaaaaa gataataact acgtatatct gacttttact acgaaacaaa
     3721 cggatttcaa agcattgggg aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac
     3781 acatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac
     3841 agattccgat tattattgat gaagagctct tattaaatga aaaacttgtt tttgcaccag
     3901 gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc atccagctac
     3961 ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca agtaaaatag
     4021 tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga aatacaacga
     4081 ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag
     4141 ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg ctttggacgc
     4201 aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat caaatgatgc
     4261 aagatgaaaa tatccaaatg atctttccgc cttggggagg agaattattg attgagattc
     4321 tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat tcagatatta
     4381 gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat ggaatcaacc
     4441 ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa agtgtactga
     4501 aaacaagaga aggagaatcg attctacagc aaccttctga aaagtatcaa aaagagtggc
     4561 cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat tggcaatcag
     4621 tcagcggaga tgcagtaagg atgaagggac gattattagg tggctgtatt gatacgatca
     4681 gtcatttaat cggtacccct tatggagatg tccaatcttt taaaagaaag tataatgatg
     4741 gaaagccgat cttgtggttt ctagaaaatt gtgagctatc gtcagttgat ttaagaagat
     4801 cacttgttca gatgaaactg gcaggatggt ttgatggcag ctcggggatc atgttcggta
     4861 gaagtgcagc agaggatttg ttagattata aaatgaagga tgtatacgaa gatctgtcaa
     4921 aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg ccacaactga
     4981 cattgatcaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg actgttttgc
     5041 aaacgtttat ttaaaaaatt ttttcctgcc ttggagttta tcaaagacca acaaatctaa
     5101 atagaaaaga aaatgaaaga agctgacagt attttgccag cttctttttg tatgatctat
     5161 tagaaatttt aattatccat aactatcaca ttgatcagct gtttcacatc atttggatta
     5221 cctgtaggaa tagctactac agatgcatta tcaataattt cttgagttgt ttctctaatg
     5281 aacgctggat aagatgtttg atctgcaggc atttgccaat gtgtaggaac tggataacta
     5341 gtgattgtcc cgtcatggtt acttgaatac actaaaactc cctggtaact gtatttccca
     5401 gtgagcataa ccacatcttt cggatagact gcactccctg catcataagg ggcgataggt
     5461 gttcctgcag gataatgaat aacatgtagt tcgaaatcac tcgatgtatc tctaaaagta
     5521 ttacctctaa tagctagcca aacacgtgcg tactcaattt gtagatcaga atatcctgct
     5581 aacggatcta ttgactgaga agactgtgag gaagttgtgt ctattgttga agaagaaata
     5641 tttgtctcaa ctgtactact agatgtagaa gaagtattgg gctctgagac tttactgctt
     5701 gttgaagatg tattcgtttc agtagaagct gatttctgag tacttgattt ttcagctgaa
     5761 ctggtttgtc tgattgaact tttgtctgtg gtcgcagatg gatcactatt ttctttacta
     5821 ggagacgtac atcctacaag gaataaagag accaacaata aaataaaacc taactttttc
     5881 atataaaata cctcattatt tttttaatac cagtatagcg taatcctgaa tttttgaatc
     5941 cgtaaaacct tgtctatgaa tataaataga gtatatcttc tttgataaat gtcagaaaaa
     6001 gtgtgacaaa taaccatcag taaaattact tgtcatacta ttgagaagga gtatttaatt
     6061 gaagagaatg attaggagaa aggatctccg tactttttat agtaatcata gaatccaagt
     6121 ttgtatagac gaatcgtttt ttctcttatt tcttctgttt caaaatggac tgcttgaaga
     6181 atcaagtctg tgaattctat aggagctgtt ccattcgcag taatcaaatt acgatctttc
     6241 acagcttgtt gttctataaa atcttcttta ttctgataat tttccgattc taaccaagga
     6301 aattgcgcat ttccggtgtg tttgtaacct tgcaataatc cgctctgagc taaataatca
     6361 actgccccac aaatagcagc tactggtacg tctgttttta acaatttatc gattatttga
     6421 tataaatgat gattttttat gtcccatgaa ttgccaccga tcataataaa aaggtcaact
     6481 ttttcaggta tttgttcgat taagtaatcg atttttgttt ttaatcctcc aatggaagtg
     6541 acctctgatt ggatagaagc agttttgttt tgccatccct ctttttggtt tagtgtgcta
     6601 gtaatataag caccttccca atcggcatat tcctctaata gaaacgtaat cgcagttttc
     6661 attttcaagc tctcctttac tatctatgac aaggacaaca gttttaacat acagacattc
     6721 attaaatttg gatagcgaca gcgaaaacaa caattcttaa gttttagaca gagtatacag
     6781 acagtcaact taattaaata cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc
     6841 agcattataa cattttgcat acatggatga caaaagaacc aattcccact tgcttgagta
     6901 caatcttaaa agaaactgga aggcaaaaag taattataga aacgaaaaat aaatcatatc
     6961 gggcactcag acatgaaaac aaccctagaa atatataact agtccaatga aaaaacagcg
     7021 aatgccgtag aaaacaagga ttctgcgtac attcatatct cattttgcga gcatatctgc
     7081 tattactgtg actaaatgtt aatatggtta aaatgaccgt tatatttttt atataaatat
     7141 cgtataatta gtatgaatat agaagaagga aagaaggtgc aatcttggaa cctaaagaca
     7201 agtatttaga ttacgctagg gtcaataaag ataaatttat tgaaacaatc atcaagggaa
     7261 aaatccctga tgatgaaaaa gatgcggtat tcatggctgg aagcccagga gcaggtaaaa
     7321 cagaggttgc actaggttta gctgaaaatt atgacaacca tgttgtgatt gatgcggact
     7381 attttagagc acaatttcca gaatacaatg gaaagaactc aagtgttttt cagaaagctt
     7441 cttcttggtt agttgaacaa tcattaaaat atgtactgga acatggctat tctttcatac
     7501 tagaggatac ttttgctatc cttagtgcag agaaaaatat tattcgagcc cttaaaaata
     7561 actttcgcgt tacgattttc tatgtttacc aagatcccaa agttgcctgg gatttcactc
     7621 gaaaacgtga actagctgaa ggacgtcatg tgccaaaaga aatgtttatc aatgcgtttt
     7681 ttaagtccag agagaatatt gaaaaagtca aaaatagaca tcctgaagta gtccttcaca
     7741 taatgattaa ggattatcaa aacgacattt ctgaagtgca ttatgcagca gataatattc
     7801 aattagtact acccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     7861 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     7921 aaacgcctca gcgaaagaat ttaaaacagt aatgaagtac gtagcagatg aagcaaatcg
     7981 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     8041 ttgagttttt tgttttaatg aattaagact attaaataga taattattgt aacttaaaaa
     8101 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     8161 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     8221 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     8281 tgtttttaca taagaagact tggggtattt tttagtttga ttagctctga tatacctata
     8341 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatgtt
     8401 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     8461 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     8521 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctgatt taattagaaa
     8581 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     8641 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     8701 ttcagagaag tttaaatgtt tcaaacaggg tggaatgaga agatctcatc aaactaatac
     8761 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     8821 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     8881 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     8941 accaaaagaa cacacgtatc tgggagaagt atatttagca ggacaaatat atactgttaa
     9001 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     9061 tcaagagcaa ttgattgaag ataaagatat atataaccag gaagaaaatc aaactagaca
     9121 tattcgtaat ttatctgatg ctaaattaga ggaagaagca agaaaagtta gtaattataa
     9181 taaaaaaatg gctgcaaaat atgttaacga agaggagttt tgcttctcaa aaacaaaaat
     9241 atataaacgt acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
     9301 ttcaaaacgt cgttgtgctc gacttaat
//
LOCUS       DAFBQW010000093         9316 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFBQW010000093 DAFBQW010000000
VERSION     DAFBQW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.096"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..2122
                     /locus_tag="LK889_002648"
     CDS             <1..2122
                     /locus_tag="LK889_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096839.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="LK889_002649"
     CDS             2119..2739
                     /locus_tag="LK889_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096840.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2853..2990
                     /locus_tag="LK889_002650"
     CDS             2853..2990
                     /locus_tag="LK889_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096841.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            3432..3812
                     /locus_tag="LK889_002651"
     CDS             3432..3812
                     /locus_tag="LK889_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK5096842.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="LK889_002652"
     CDS             3794..4708
                     /locus_tag="LK889_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK5096843.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="LK889_002653"
                     /pseudo
     CDS             4948..5637
                     /locus_tag="LK889_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5757..5843)
                     /locus_tag="LK889_002654"
     CDS             complement(5757..5843)
                     /locus_tag="LK889_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK5096844.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6488..7225
                     /locus_tag="LK889_002655"
     CDS             6488..7225
                     /locus_tag="LK889_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK5096845.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7253..7792
                     /locus_tag="LK889_002656"
     CDS             7253..7792
                     /locus_tag="LK889_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK5096846.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            7808..7978
                     /locus_tag="LK889_002657"
     CDS             7808..7978
                     /locus_tag="LK889_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096847.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(8290..8751)
                     /locus_tag="LK889_002658"
     CDS             complement(8290..8751)
                     /locus_tag="LK889_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5096848.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8884..9258
                     /locus_tag="LK889_002659"
     CDS             8884..9258
                     /locus_tag="LK889_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HBK5096849.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3596 a         1413 c         1455 g         2852 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt tttaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt tcaggttgat gtcgccttta ttgattttac
     5761 ttcttgtttc ggttattcaa ccaataactg aataaagaga cacaaactcc tacaagaagg
     5821 gggcaaatga acagttcaaa cattagatac acctccttcc agtcgcaaga ctgtcggata
     5881 ggcgacaagt tattatatct taaaaaatta aatttgtgtg gtgtttttaa gtatcacttg
     5941 ttttagggtt gttagggctt gccctgaccg cctgtaaggc gcttctcgac tagattaagg
     6001 gaaattaatc aagaattgtc ttagaacggc ttagacaagc tttcagagtc tcacacctcc
     6061 tctttgaaca acggttttag caaaggagcc ggaggcgact gcgcagtata atatgagagc
     6121 atagcgatca caccttttta gcgaccgtag ggagcaagtt ttccagttta gaaaaattcg
     6181 cgaacctttt tggaggaaaa agtaggagtt ttttaggcgg gaaattcaat gtgctcttaa
     6241 gatatactgt tagatatact gttagatata ctgttaataa taactgttag ggattagcaa
     6301 ccttatggga gagtaagtat tgacataggt agtgggggat aaattgtcac actatggggg
     6361 ataaattgtc acactatggg ggataaattg tcacactatg ggggataaat tgtcacacta
     6421 tgggggataa attgtgtttt acatcctctt atgtctatga taacataatt tattgaggtg
     6481 atttttattg agtaacgata tagttcgata taataatgga ttaaattctg tccctttaag
     6541 aaaatttacc cctatagata tggatttatt ttggggaata tgttctaaaa tgaaaagaaa
     6601 aggtacaaaa gaagaaattt ttacatttaa acaaataaaa gaattaattg attacaatat
     6661 aaatgcacgt ggcgaccgtt ttgctaatga tttaataaaa atgtcaaaaa aaatcattac
     6721 tttgtctttt atttatgaag acgaaaaaac atatgaacaa ttaaatctgt ttcaaaaatt
     6781 taaaatcgat aaagtgaatg aaacattatt aataaaggtt tctgaagaat ttgaatatat
     6841 acttaattca atcggaacta attttactcg tttcgaatta gaaaatatga caaacttaaa
     6901 gagtagctac gttaaagagt tatatcgcca acttatggct cataaagaca gaagatctaa
     6961 aaaaggtcac tggtttgtaa aaatagaaga ttttagagct ctattagcaa taccagaaag
     7021 ttatcgcatg agagatattg ataaacaaat ttttgcccaa gcgaaaaaag aatttttaaa
     7081 cgttaattca actcaccctg ctatttttaa aacttttgaa atcaaaaaaa ttaaagccta
     7141 caaaggaaat aagataggat ctctgcaaat ttattttgaa gaaaatatac cttcaattac
     7201 cttagataat tggttagaga aataatacct aacaatttag gaggcaatta tcatgcaacc
     7261 caaagaaacc tttacaataa aggaattatc tgaattattt gaaataagta ggcaagcaat
     7321 ctctaaacat gttgataaat tagacccgtc cttattagct aaaaatgagc gtggttataa
     7381 ggttgtatta cgttctggag tattgcaact tgctagcaac ttaggtaacc aaaggttact
     7441 atataggttg catgagtatg aacaagaaaa cgataaaaac gtttatatat caagcaaaaa
     7501 cgtgattgta aacgagttgc tagataagtt gcgtgaagaa aatgaatttt tacgagaaca
     7561 actttccctc aaagagcaaa ataattctaa tatgcaaaaa ttactcgacc agcaacaaca
     7621 gctcacccta caggcaaatc aacagatcga aaaactgcaa gaacagttac aattagctta
     7681 tacggaagaa actccagaag atgaatcaac tactattctt tctgaagaaa cagaatcgat
     7741 agaaaaacac acaaaaaaga agaagcaaaa atggtggcaa ctttggaaat aactggaggg
     7801 aacattcatg acaagaaaaa atcttctatt cggttctatt ttactaggaa ttctaggctt
     7861 cagtccgatg ttattgtcag taatcaataa agggaataaa catggtgaat tgaaattgaa
     7921 tccaaattat tacaacagtc ctaacgaaga attgtttgta aaagagacgg ataaataata
     7981 aaaaagactg ctcttccgag cagccagtta aaaaataatg agtgtttatt cttgaaaaag
     8041 cacgcgctta tgaagcgcta ccaaaaattt accatatttt acaaaaaata acaagtctta
     8101 aaaaaatgac agataaaaat gcacaaagaa ttagatatag ataaccgcga aaaatcaagc
     8161 tttagaaagt tgcttttagc tagaccaatt aaaaatcaca aagtgagcgt ctctatccat
     8221 tggtagaggg gctacttggc atagtgtaca ttatgtcaac tatgcaatta aataactaac
     8281 tacttcatat tagcttaata aaataatcaa aataactagg gggaaaaaca ttgtataaaa
     8341 aaatttttca ttttttaaat aagaatagta aatataaaaa taatttaaga tatctattac
     8401 tattaatcca gatagagcaa accaagtaaa tattccatgt gtaaagctaa aaaaattcat
     8461 ttgaatcaat tcaaataaac ctaatatagt aacaaatata ttaaaaataa tagctgtcaa
     8521 taccagtaaa ttattttttt tattttttga attatcttca tttaaaatag aatttatgtt
     8581 agtactataa aattcacaaa ttttttttag tgtctctata tctggtgttg ttttgttatt
     8641 ttcccacttt gaaatagttt gacgtgtaac gttaaatttt cttgccacat attcttgagt
     8701 ataccctttt ttcactctta attctcttag cttagatcct aaatcctcca ttttttctcc
     8761 tatgtaaacg attatttaca taaaatttta tcataaaaat actaacgcgc caaaaaagtt
     8821 ctttatttta taaaacacac taaaataaaa taattctagt aaaaaagata ggagaaataa
     8881 gttatgaaaa aatttttatg tctttctgct ttaatgggtg tcttattaac tagtggagga
     8941 atagctagtg ccacagaagc tttaaatcta gatgtacccg aagaccatac cgccatttac
     9001 ggaggaggaa tggaagctac agaagaagga ttttcttcta gtaaattgag atatgctgct
     9061 ggcggcgggg atttcaactg tggggttaat ggatttaaag tttatgcaaa ttactaccat
     9121 gctagagcta aacatagtgc aacagctaaa aatggacgag gcggtcaagt acgatcagtt
     9181 cagaaagctg gagtaagagc ttatgcaaca tgcaatgcca cacttactgg aaatactggt
     9241 tggtggaatg tttattaaaa aagagagttt aaactctctt ttttatttac atttgaaagg
     9301 aaattcaaaa tatgtt
//
LOCUS       DAFBQW010000094         9209 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFBQW010000094 DAFBQW010000000
VERSION     DAFBQW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.097"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            470..2107
                     /locus_tag="LK889_002660"
     CDS             470..2107
                     /locus_tag="LK889_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096850.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LK889_002661"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LK889_002661"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK5096851.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="LK889_002662"
     CDS             complement(3183..3818)
                     /locus_tag="LK889_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK5096852.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LK889_002663"
     CDS             complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LK889_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK5096853.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5404..6400
                     /locus_tag="LK889_002664"
                     /pseudo
     CDS             5404..6400
                     /locus_tag="LK889_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6416..8434
                     /locus_tag="LK889_002665"
     CDS             6416..8434
                     /locus_tag="LK889_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBK5096854.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8494..9186)
                     /locus_tag="LK889_002666"
     CDS             complement(8494..9186)
                     /locus_tag="LK889_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK5096855.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT         2978 a         1706 c         1445 g         3080 t
ORIGIN      
        1 actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa
       61 gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata
      121 gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga
      181 agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg
      241 agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag
      301 aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag
      361 tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag cactagtatg
      421 atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa
      481 caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt acgtcatccc
      541 tctattcatg tctatgggaa tctatcatag tgtcaatcag gatacttact ttcatttatc
      601 tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata acttcgatca
      661 tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag cttttttatt
      721 ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt ttatcacttt
      781 tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc gttatatttc
      841 gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca tattccatcg
      901 tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa tggggtgtta
      961 cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca tgacgctagt
     1021 agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag gaacgttgtt
     1081 tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt taaaagctac
     1141 cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac aatctagagc
     1201 acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta gtgatatgct
     1261 gacacacatt ttaaataata actataacaa ctatactatt ggattattct tatttctcgg
     1321 attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat ttattttctt
     1381 tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac tttttaacca
     1441 tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataaacgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagcg attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga
     8461 taaaagacat gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg
     8521 attaaagcca tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg
     8581 attcccctga ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag
     8641 atagacgtgc tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg
     8701 atagtctgct tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat
     8761 agccatcttc cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac
     8821 actcatttga ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat
     8881 aaatgcctct aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct
     8941 gctttctgaa gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
     9001 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc
     9061 agttgacttg ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc
     9121 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
     9181 accaattgtt actaaaatcc tttttttca
//
LOCUS       DAFBQW010000095         9042 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFBQW010000095 DAFBQW010000000
VERSION     DAFBQW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.098"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1670
                     /locus_tag="LK889_002667"
     CDS             <1..1670
                     /locus_tag="LK889_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBK5096856.1"
                     /translation="GLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVV
                     VIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNK
                     YLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVI
                     YFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFV
                     SDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTN
                     NDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTE
                     HDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDF
                     AGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRN
                     SDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGII
                     SFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            1750..2145
                     /locus_tag="LK889_002668"
     CDS             1750..2145
                     /locus_tag="LK889_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096857.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            2470..3087
                     /locus_tag="LK889_002669"
     CDS             2470..3087
                     /locus_tag="LK889_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5096858.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3128..3427
                     /locus_tag="LK889_002670"
     CDS             3128..3427
                     /locus_tag="LK889_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBK5096859.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            3461..3628
                     /locus_tag="LK889_002671"
     CDS             3461..3628
                     /locus_tag="LK889_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5096860.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(3673..4278)
                     /locus_tag="LK889_002672"
     CDS             complement(3673..4278)
                     /locus_tag="LK889_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5096861.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            4403..7390
                     /locus_tag="LK889_002673"
     CDS             4403..7390
                     /locus_tag="LK889_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBK5096862.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            7539..7748
                     /locus_tag="LK889_002674"
                     /pseudo
     CDS             7539..7748
                     /locus_tag="LK889_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7883..8065
                     /locus_tag="LK889_002675"
                     /pseudo
     CDS             7883..8065
                     /locus_tag="LK889_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            8041..8397
                     /locus_tag="LK889_002676"
     CDS             8041..8397
                     /locus_tag="LK889_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096863.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
     gene            complement(8911..>9042)
                     /locus_tag="LK889_002677"
     CDS             complement(8911..>9042)
                     /locus_tag="LK889_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5096864.1"
                     /translation="MREDTELKNFPLFCPKCRQEILIEITKFRITVITEPDAKTQSR"
BASE COUNT         3070 a         1383 c         1682 g         2907 t
ORIGIN      
        1 atggattata tgttaatggt tattttgaag acggtacatt cgatggtatt ttcactaaat
       61 atatgggttt agaagtactg gcattccgaa aatttatggt attgcctgaa tatactgaaa
      121 tagcagatgt tgttgtaata aaaggactat tgtctagcgc tggagatatt agaagatctg
      181 cagatggcgc agatacttct acgcaattaa ctagatctca aatccaaaca ttggttgata
      241 atgatataaa aatagttggt agatatctta ctggtactgt tggaattgga aaagatgaac
      301 gaaataagta tctaactaca gaggaattga ataatatttt ttctaaaaat ttatctgtgt
      361 ttcctatcta tcaagatgga ggtgcagcat tagcttattt tacttatgat cgtggcttat
      421 cagatggtaa gaaggctata gatgcagcaa aaaatctaaa tataccatta acaacagtta
      481 tttattttgc tgttgatcta gacatgttag gggaagaaat tcttgcatat gctggagaat
      541 attttaaggg ggtatctgct gtaatgtcat atagcgggta tcaaacgggt gtttacggaa
      601 caagaaatgt ttgttctcaa ataatcaata acgggtatgc ttcgtttgca tttgtttcag
      661 atatgtcgac tggttatagt gggaatttag gttttcctat gcctagacaa tggtcttttg
      721 atcaatttgt tgaatttacc ataggaagtg gcaacggagc tgttggtatc gatttaatcg
      781 ctacatcggg tagagatagc ggatttaatg agttatcaaa tgatactaat aatgataatt
      841 acattgccaa atacaatcag aaggttataa atcagatggt acgtgcgtat caatatctat
      901 cacaagctgg tcttgataat ccttggaacc ctcatttgac attttataga tatgtaaatt
      961 attctggttt gtcatgggac atcatttctt ctcctgtaac agagcatgat agaaaaattt
     1021 atgatgaata taggaataaa ttaactaata gcgagggcct gtataattat tttatagatc
     1081 ctaatactgg ttcaataata ggtttgcctc acttaattgt gacgttacaa tcacagatgt
     1141 ttctagccga cacaatcaat gatagtgttt ctgactttgc aggttggtta ggagatttaa
     1201 tgacttgctg gggtgaagtt aagaaattag gaatacaaat ggagcaaggt gtatttgcat
     1261 tagtaggtac ggctgcaggt ggaacattta gtctagaaga tttataccaa gatgttgacg
     1321 cggtaaatgt ttatacctta gcaaaaagaa attctgattc aacagcttta tcggctttaa
     1381 aaacgtacta ttgtgataag ggtgctattg atagatttaa tcaatttgtt aagaacagat
     1441 ttggttcttc agataaaatc gcttcagtaa caaatagttt attagaaaat gacgaaacag
     1501 ttgatatctt aggtgtaggg ataatttcat ttttcattga taaaatagtt aataattcac
     1561 ttgggacaga ttttacttat cttgatttta ttggagatga ggggaaagtt gcaaatggat
     1621 tttctgaaaa aattattcaa tttcaagaaa atagaagtaa acttaattaa gaatcgttta
     1681 ctatttaaac agtgtccttt tcctttttaa atatatacga taaaataagt atatgaattg
     1741 taaaaggaca tgatgattat ggaagaaata gaatggaata atctgactca aaataataaa
     1801 aataattctg gatgtggaat tatctttatg tttgttatag ttttgggaat agtagtactt
     1861 ttaataactg tcaagatagt agattacttt gcgaatactc ctgaagaaca aatacagaga
     1921 aaacttcctg agtttgaaag agtaccacct tatgatccaa gagataattc atttcaagta
     1981 aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag ttacagttga atcaagcgac
     2041 ttcaaagaaa aaaagaaaga atacaattat attgtaaagg caaaagtatc aaaaagattt
     2101 atttttaacc ctaaattaga aatatatagt gttgtaatag aataatagtg ttaaaaaaat
     2161 tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa aatataccat ggaagacata
     2221 aaagaaaaac tggataacta gtttatttaa tggcagaccg ttaaggcctg ccatttttat
     2281 gtattttagc ggaataaata taatcatgcg ggagtgtaaa cttactgttt caaaaagtat
     2341 actttttgac atgccttgat tttctttgtt tatgtatggc ataataacca cataatacaa
     2401 tttgaaacgt ttctgattta atctgattgt tttgaaacaa ttttagtgaa aaaagggtag
     2461 ataaaaacga tgagaaaatt tttttatatc cgggtcagta gttatgatca atcgatcgaa
     2521 cgacaactga tttccgcgaa ggaattagga atccctgaag aatatatatt tgtagaaaaa
     2581 gccagcggca aagattttaa acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa
     2641 ggagatttac tctatctaca atcattagat agactaggca gaaataaaca aatgatttta
     2701 gaagaatggc gagaattaac taaggaaaag cagattgata tttctgtgtt agacatgcct
     2761 ctgttagata ctgctagtta caagcaacta gatgggttag agacgcttat ctctgactta
     2821 attctgcaat tactcagcta tatggccgaa gacgagcgga aacgaattaa tagccggcaa
     2881 agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct
     2941 ttacctcctg aatttcctgc gatctattct gactggaaag caggaaagat aactgcagtc
     3001 tatgctatgg atcaattggg catgaaacga aatacctttt atcgacgggt gaaagaatat
     3061 gaacatacct tgcaccactt ttcttgatag atataacagc tattagacgg gaagggagag
     3121 tttgaaaatg gacttaacga atcaaagggt cgtttttact ggaacgctgc agcaaatgac
     3181 acgaacccaa gccatcggtt tggttcactc tttaaacggc cattgtcaat caagtgttac
     3241 ttcaaaaaca aatttcctgg tttgtggtca gtattctaag tcgttgtttg atgattctct
     3301 ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg ttaggtcatg agattaccat
     3361 tttatcagaa gtcgaatttt atgcgttaat tagtcaacag ttgaaagaat ggcaacggta
     3421 tttataatcg ttagtaaaat ttacaaggag gaaaattttt atgaagcgaa aatcttatga
     3481 tcgttggggc attgaaataa ctagttttaa tcgaaatatt gcttttcacg tggagtgtga
     3541 ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac ttttactgtc ctaaatgcca
     3601 tcgccgatac ggggtacatg gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa
     3661 tgtagtatat ttctacaaat cattcgcttt ctttatttta taaatagttg gtcgagagat
     3721 cccgttttct tttgcaatct ctgcaactgg aatgccttgc tttaacatgt ccaccacggt
     3781 tttaaaaact aagcgttttt gcggatctgt tgagttaata gaatactcga ttggtcgtcc
     3841 cttatatcgg ccggcttttt gagccaataa tactccttgc cgctgacgtt cacgaatttt
     3901 ctcacgctcg ttttgtgcaa tataacttag cagtgataaa aacatatcga acatagcggt
     3961 atccaggagt tcatttccag tattaaaatc taaaaactgt gcatctaata tttttaacgc
     4021 tacttttttc tgtttcataa aagcaactgt attttttata tcttcataat ctcttcctaa
     4081 acgatctagt gatgtaacga tgacgcaatc accttcacgg atataattta gtaatttttg
     4141 aaactcagga cgctcagtat ctttaccact ggctttttct tggaataatt tatcaatgtt
     4201 aagtccttcc atcattgcca attgtcgatc caaattttga tcttgtgaag atactcgaat
     4261 atagccaatt tttcccataa aaaactcctt tttaaaagtg atataattta catgtaaaat
     4321 ttacttgtag ttttactgct atcatagcat ttttaaaaat aaattttatg tagacttaaa
     4381 atttacttca tggagggttt aaatggatga aatcaaaagt aacaaaaaag catttggcat
     4441 ttatttagga gaacctagta cagaacaatt aaaccaattt ttttatttgc atgaaaaaga
     4501 tttagaattg atggagacta tacgacgacc agctactaga ttaggttttg cactacagtt
     4561 agtctcacta cgttttttag gaacttttat attagatctt accaatattc cccaaaatgt
     4621 attgagttat gtagcaactc aactaaatat tgatcctgga gaaattatct actatagcag
     4681 gagacagacc aggtatcagc acacaaaatt gataaaatca cattatggat atcaagattt
     4741 caaccaaccg gaaatagaaa catacctagc taactggctt ttaaatagag cgctgtatac
     4801 aacagaatct tcagatatgt tgtttgatat gcttctaaaa aaatgtctgg atgaaaagat
     4861 tgttttacct ggaattacta ctttttctcg ttttgttgct agtgcaattg aaaaagctga
     4921 agagcattta taccgacaat tgtctttggt tccaactgaa gatgagagaa agcggcttct
     4981 caatcattta gaattaattg gaactccagt ttatggggca ataatcaaaa tggacatttt
     5041 gagaaccccg ttagctgacg atagtcataa agaaatagcc agaggttttg accgactaaa
     5101 gcaatttcaa gctttctcta ctgaaaaatg gaagatggaa tctattcctg aagggaaaat
     5161 aaaagttctt gcaaattatg catttaaagc taaagctcaa ttaattcaac gaatgagtga
     5221 acagaaaaaa ctggcgttgt tagtggcatt tattttcata tacaaacgta aggctatgga
     5281 tgaacaaatt ttagcactat ctaatttttt tgaaactgtt tttagacgcg ccaaaaataa
     5341 ggaatctaaa gaacgtcttc gtaccataaa agatctagat cgagccgcat cgacactttc
     5401 agatattgta gatttagtaa tgtccagtca ttctgatact aatgaactat cccttcgcga
     5461 tcgtatctta gaaaaatatt ctgaggaaaa tgttaccgaa gctgtatcgc aagttcggct
     5521 attggttaaa aatgatcgtg aaccaatagc catttctgaa cttctgaaca cttatcgaaa
     5581 gttcagaaaa tttattccgg atattttaac tacactaaca tttgacagca atgactacgg
     5641 taacaactgt aaggaactct ggaccttcat taataataga tttcctaagc cgattactta
     5701 caaaatgttt ggagctatta aacatactct tccaaagaaa tggatttatt acattcatga
     5761 ataccctgaa caaactaata aatgtgtatt gattgcagct gtcgaattgt taattcaatc
     5821 tttaaaacgc catgatattt atattcctca gagtgtctta tataacaatc ctatggattg
     5881 cttgattgat gagaaaactt gggttcaaca aaaatacgtt ttattagaac aactagatct
     5941 tccttcttca gcaaatgaaa tgtctttatg gttaaagaac gatttggaac tgtcttataa
     6001 agaagcgcta aatcgttggc ctacttctaa aatggccaga atagaaaatg cagatcgcct
     6061 ggttgtctct agacttaaaa agacatatga aaaaaaggaa gataagtctt ttagacgtcg
     6121 cattcagcag ttacttccta aaattgattt gtcagatttg ttattggaag taaaccaaca
     6181 gttaaatctt actcaatcat ttaaccactt aagtgacaag gacaccaaaa tgaaagactt
     6241 ggatattagt ttattagcag ttcttttatc agagtcctgc aatattggtt tttccccggt
     6301 ttcaaaagaa ggatttgatt ctttaaagta tgatcgtctt gtctatgtca atcatcatta
     6361 tattcgagtg gatacacttt caggagctaa tcaaaaaata atacatgcac acaggaaact
     6421 tgccacctct aagatatggg gtgatgggca tatggcttca gctgacggta ttcgttatat
     6481 cacaccacaa cgttccctat attctagaag taatccaaaa tattttggcc gtggtcgtgg
     6541 gatcacattc tacaattttg tctctgatca atatataggg ttccacggaa tggtcgtttc
     6601 agggacactg cgagattcgc tttaccttct agaaggattc ctcaatcaac ccagcgaatt
     6661 aactcctaat cagatcatga ctgatacagc tggttatagc gatatgattt ttggtctctt
     6721 tggcttatta gggtttcaat ttagtccaag aattgccaat gataaaggaa ccaaactttg
     6781 gcgaatcgac gctaacgctc actacgggga acttgatacg ttgtctcaaa attcaattaa
     6841 tatggaactg atttataccc attgggagga cattcttagg gttgcaggtt cgttgaaatc
     6901 tggaaaagtg aatgcaacag aattaacgaa atctcttcaa cggaatgggc aacccacgcc
     6961 tttaggaaaa gcaataactg agtatggaaa agtttataag acgaaacatc agcttcgtta
     7021 tatttctgac gaaatatatg cacggcaaat tttagaacaa ttgaataagg gggaagctcg
     7081 gcatgcctta tgccgtaata ttttttatgg acgaaaagga aaactctacc agacttatta
     7141 tgatgggatg gaagagcagc tcaatgcatt aggattagtt actaatgcca tcatatattg
     7201 gaactcactg tatttagaaa aagtatttga acaaatgaaa gctgaaggat tcgactgttc
     7261 cgaagaaatg atacgcaggc aatctccact aattagtgat cacatcaatt ttgttggtaa
     7321 gtatacgttt agatacgaag ctgaattatc caacggtaag ttacgtccat tgaatgtaga
     7381 agaattctag caatgttcgg gttttgcatg ctaccgcact atttcgtatg actgttccag
     7441 cgaatcactt cgaatagaaa aagagaaaaa cacgagaaca gcgttaaatc aacgtttctt
     7501 gtgtttttac ttttgtgcta ccgcactatt tcgtttctat gttccatgta cccctttatc
     7561 aagaagcgca aacacgccaa attgccgatt ggttgatcgg catgaacgca agccctttat
     7621 ataccttgaa tttacaacaa aaaggcgtac aaggtacatt ttcattagga cgtgtacaaa
     7681 caccgacttt atatctcatt tatcaacgcc aggaagcaat agaaaacttt aaaaaagaac
     7741 cttttttcct gaataattca tagttttact aatagtcttt cctaacattg atttaatagt
     7801 ctttaaaggg ttctgttgca aagttttaaa tctactatca aataaggtag aataatagaa
     7861 aaagatagca ggaggaatga cgatgaatca ttttaaagga aagcaatttc agcaggatgt
     7921 gattattgta gccgtgggct actatcttcg ttataacctt agctatcgtg aagttcaaga
     7981 aatcttatat gatcgtggca ttaacgtttc tcatacgacg atttatcgtt gggtgcaaga
     8041 atatggcaaa ctactctatc aaattttgat ttcaaaccat tgggtcttgc gattaaagaa
     8101 gcccgtctta gtaaaggatt gactcgtgag caggtaggat caatgatcca gattgcgcca
     8161 cgctatttga cgaacattga aaataaagga caacagccaa gttttcatgt tgtatatgat
     8221 ttagtgactt tgctaaatat ttctttggat ggatttattt taggggaaga aaacagtcat
     8281 aaaagctcca aacgtcttca agtagaagca cagttggatt gtctggatga agatgaatta
     8341 gtgacgattg ggaaagtgat tcaagcaatt tacggagaaa aatgtaaatt taactagttc
     8401 aataatacac aatggattaa ttaatactat caggaaagat tgattacaaa gatgtaatga
     8461 attttttctg atttttgtta atcataaata gaaaatgaat ttatctttgt tacacttagg
     8521 aactagagcg aatgataaat agttctttgg ttactcttat cgctgcaaag tacagttcat
     8581 tttttgttgt tgggaaaatt ttctggattt attgcatatc acttcatccg actaaaaatt
     8641 tttgattccc atagcgaagt tcgtttttgt tccttaggaa aaacttgact gatatactag
     8701 ttttgaaatg acgagtaact ctgtacttca tcaataatca agtattgaat tttaattgta
     8761 tactttttat gcttgaggga tgtctactgt aatacagcta ctgcaaaaaa aatcagtaca
     8821 cttattttct tataaaggat atcaagaact aagctcaatg gtatgcatag aaaagaaaaa
     8881 gccgataaaa tgagattttg atatctcgaa ttatcggctc tgcgtctttg cgtctggctc
     8941 tgtaattaca gttattctaa acttcgttat ttcaattaaa atttcttgtc tgcatttagg
     9001 acaaaataga gggaaatttt ttaattcagt atcttccctc at
//
LOCUS       DAFBQW010000096         8909 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFBQW010000096 DAFBQW010000000
VERSION     DAFBQW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.099"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            191..709
                     /locus_tag="LK889_002678"
     CDS             191..709
                     /locus_tag="LK889_002678"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5096865.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            837..1520
                     /locus_tag="LK889_002679"
     CDS             837..1520
                     /locus_tag="LK889_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBK5096866.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1829..1902
                     /locus_tag="LK889_002680"
     tRNA            1829..1902
                     /locus_tag="LK889_002680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Arg,seq:tct)
     gene            2061..2420
                     /locus_tag="LK889_002681"
     CDS             2061..2420
                     /locus_tag="LK889_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBK5096867.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2543..4174
                     /locus_tag="LK889_002682"
     CDS             2543..4174
                     /locus_tag="LK889_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBK5096868.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4236..4877)
                     /locus_tag="LK889_002683"
     CDS             complement(4236..4877)
                     /locus_tag="LK889_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK5096869.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVPPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5063..5139
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5159..5467
                     /gene="rplU"
                     /locus_tag="LK889_002684"
     CDS             5159..5467
                     /gene="rplU"
                     /locus_tag="LK889_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK5096870.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5494..5838
                     /locus_tag="LK889_002685"
     CDS             5494..5838
                     /locus_tag="LK889_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBK5096871.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5851..6144
                     /gene="rpmA"
                     /locus_tag="LK889_002686"
     CDS             5851..6144
                     /gene="rpmA"
                     /locus_tag="LK889_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK5096872.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6237..7385)
                     /locus_tag="LK889_002687"
     CDS             complement(6237..7385)
                     /locus_tag="LK889_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5096873.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7397..7708)
                     /locus_tag="LK889_002688"
     CDS             complement(7397..7708)
                     /locus_tag="LK889_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096874.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7761..8156)
                     /locus_tag="LK889_002689"
     CDS             complement(7761..8156)
                     /locus_tag="LK889_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK5096875.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8192..8536)
                     /locus_tag="LK889_002690"
     CDS             complement(8192..8536)
                     /locus_tag="LK889_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5096876.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
BASE COUNT         2733 a         1455 c         1704 g         3017 t
ORIGIN      
        1 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
       61 ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc
      121 tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat aaagaaagaa
      181 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
      241 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
      301 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      361 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      421 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      481 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      541 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      601 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      661 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      721 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      781 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      841 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      901 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      961 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
     1021 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
     1081 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
     1141 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
     1201 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1261 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1321 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1381 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1441 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1501 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1561 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1621 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1681 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1741 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1801 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1861 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1921 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1981 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     2041 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     2101 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     2161 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     2221 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2281 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2341 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2401 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
     2461 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
     2521 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
     2581 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
     2641 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
     2701 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
     2761 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
     2821 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
     2881 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
     2941 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
     3001 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
     3061 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
     3121 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
     3181 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
     3241 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
     3301 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
     3361 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
     3421 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
     3481 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
     3541 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
     3601 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
     3661 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
     3721 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
     3781 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
     3841 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
     3901 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
     3961 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
     4021 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
     4081 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
     4141 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
     4201 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
     4261 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
     4321 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
     4381 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
     4441 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggcggg
     4501 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
     4561 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
     4621 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
     4681 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
     4741 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
     4801 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
     4861 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
     4921 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
     4981 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
     5041 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
     5101 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
     5161 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
     5221 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
     5281 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
     5341 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
     5401 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
     5461 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
     5521 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
     5581 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
     5641 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
     5701 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
     5761 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
     5821 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
     5881 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
     5941 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
     6001 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
     6061 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
     6121 tatccagtag ctcaagaagc ttaataaata aactgctcta tctcttgtgt agcaagggat
     6181 ggagcttttt tgtaaattat gggatttttt ttgaccatac ttttgaccat actttcttag
     6241 aagttaatat aatttgttag tttttgtgtt gtttcttctt ttttgctgtt agtaatgtgt
     6301 gtatatgtgt ccatagtagt ttttatattt gcgtggccca ttctttcttg gacttctttg
     6361 tgatctgcgc ctgcctcata tagcattgat gcatgtgtat gtctaaaacc atgcagaccg
     6421 atataaggta agccggcacg ctcacgatat atattgtatc tttctgccac tgactgatta
     6481 attgtgaaat tattttcgtc attggtaaac aaataattag ctttagtaaa tcctaattga
     6541 aataagtact ttctttgttc gagcttccaa aattttagtt gctttatagt tttttcatct
     6601 aaataaatta ttcgatttga ttttttggtt ttaggagttt cagatatata atatttatct
     6661 gaacgagcta cagttttttt aatggccatt tcatttgttt taaaattaat attgtcccaa
     6721 gtgagtgcta atatttcgcc gattctacat ccgctgaatg ctaatagtct aaagagcgta
     6781 tagtctctgt ttttaaaata tggattctgt tcttgagata cttcatttag aaataattcc
     6841 aactgttctt ttgtatataa ttttaatttc ttttctgttt taatttgtgg ctttggaata
     6901 agtaaattta atgttggatt atcagatgtt aaaccaatat ttatagcata cttaaaaact
     6961 ttattcatat agtttatgaa aagagggtac tgctttgaag ttcctttttc actccaatca
     7021 tttactacct tttgacaata tgccgtatta attttagaaa gcttaatatt tccaaatttg
     7081 ggatatatat gtttattaaa gattatttta gtccttgaat atgaactttc ttttacagtt
     7141 tttttgtagc tttctagcca taattctgct gcttctttga aagtagtatt tgtacttgtg
     7201 ggcattcctg ttttttgtaa ttctagttct aacctagaaa gtgctgtttt tgcttctttt
     7261 tgtgttctaa atcctcgttt agtagtatat tttttctttc cagttagcgg atcaataccg
     7321 agataagttt taaagtacca tgctttttcg ccatttttct ttttatattg ttttatcatt
     7381 gccatcaata ttacccctat tcaaattcta attctgccat gctatcattg aaaaattcga
     7441 tcagtccttg ttcctctata aattcaagcc atatttcctt cccgttaatt aatggaactc
     7501 ctttttcttt acgaattgtt tgatacaagt tagtcacgac ttgtttccac atttctaata
     7561 cagctttatc tattttctta tcatcccagc gttttttttg gggtctattt ttgttttttt
     7621 caatttgata attagtagct ttgatgataa atgtttcgta tccggttgtg cttttttcta
     7681 accattcttt gaattctgta tatatcattt ttgttcacct ttctattttt gaacgtatgt
     7741 tcttttttgc ttaaaaaatt ttagagagct gcataacaat agatgcgagc ttttaataat
     7801 tctttcacaa gtggttcgaa gcgaacgctt aaattgttat cttctataaa tttcatgtaa
     7861 ttgatattgt gtggttctaa ttcagacttt gcaatatatc tatctaataa tttttcaatc
     7921 atgaagcgat cagcctcata ttccattttt gaatggagag agaatgctaa gttatataaa
     7981 aaataattat catgatgttt agaagcgtgt cctaattcat gtaataaggt tctttgtctt
     8041 ctatacttag ataaattagc ttttattact atggtattca tacaagcaac ataatggcca
     8101 tcggcatcaa ggttttcacg ctcttctacc ttaacgccga gttcattaat tatcatttca
     8161 atttgactgt ccaaatgact cacctactta tttagttatc ttttttctct aaatatgctt
     8221 caatcatagc tgatagtatt tccctatcat tatcggtgag cggttttccg tcgctactca
     8281 taacagatgc taaagcttct tccacggtta gttttctttc ctctggtgca actcccgcgt
     8341 taggatcatc tgtgcgtcct agcaaataat ctgttgatac atggaagtag tcagcaactt
     8401 tttgcaaagt ttctatttta ggactttgtg tcttccattt ataaatagag ttttttccaa
     8461 atcctattct attttctaat tcagaaatag aaatacctct ttttttacag agctctttaa
     8521 ccctttcgaa aagcatgtca aatcaacctt cctagagcgt acgataataa tttatacaaa
     8581 aagatagaaa ggtagttgac aaatctatcc aattgtctta tatttaactc gtaagctaat
     8641 ttaataagct aacaagcaac aagaaataca cctaataaaa taaacgaatt cgaggtcggc
     8701 aaactaagaa tgttaatttt tagggttttt ctaggcttat ttaactgtat ataaatataa
     8761 gacagttgga tagaaatgtc aataaaaatt agctaatttt attagcttac atatttaaag
     8821 aaaaggagga aacatcatga aacaaaataa aaaaactcct tccgcaaaag gagctttaat
     8881 aactttaaat tttaaagacc aaaagttta
//
LOCUS       DAFBQW010000097         8781 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFBQW010000097 DAFBQW010000000
VERSION     DAFBQW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.100"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(56..349)
                     /locus_tag="LK889_002691"
     CDS             complement(56..349)
                     /locus_tag="LK889_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBK5096877.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(377..1042)
                     /locus_tag="LK889_002692"
     CDS             complement(377..1042)
                     /locus_tag="LK889_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBK5096878.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1182..1814)
                     /locus_tag="LK889_002693"
     CDS             complement(1182..1814)
                     /locus_tag="LK889_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5096879.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1821..2888)
                     /locus_tag="LK889_002694"
     CDS             complement(1821..2888)
                     /locus_tag="LK889_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5096880.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2885..3616)
                     /locus_tag="LK889_002695"
     CDS             complement(2885..3616)
                     /locus_tag="LK889_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBK5096881.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3785..4264)
                     /gene="greA"
                     /locus_tag="LK889_002696"
     CDS             complement(3785..4264)
                     /gene="greA"
                     /locus_tag="LK889_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK5096882.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="LK889_002697"
     CDS             complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="LK889_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBK5096883.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5779..6948
                     /locus_tag="LK889_002698"
     CDS             5779..6948
                     /locus_tag="LK889_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK5096884.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7215..8129)
                     /locus_tag="LK889_002699"
     CDS             complement(7215..8129)
                     /locus_tag="LK889_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096885.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTL
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(8187..8781)
                     /locus_tag="LK889_002700"
                     /pseudo
     CDS             complement(8187..8781)
                     /locus_tag="LK889_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2781 a         1682 c         1322 g         2996 t
ORIGIN      
        1 atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt
       61 gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat
      121 atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca tcaatttcca
      181 ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa
      241 cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa
      301 tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct
      361 actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct
      421 ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga
      481 taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat
      541 ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag
      601 aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa
      661 tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta
      721 ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta
      781 ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat
      841 cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag
      901 catgagtggc tatattcata tattcattga cccgatcttc attactatca atagccagta
      961 cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta
     1021 caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac
     1081 aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata
     1141 gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt
     1201 ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg
     1261 gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta
     1321 ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt
     1381 aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct
     1441 aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag
     1501 gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca
     1561 ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt
     1621 gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt
     1681 gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct
     1741 tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc atctactaat
     1801 aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt
     1861 gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc tactcgttct
     1921 ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg
     1981 tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca
     2041 ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga
     2101 tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga aatctggatt
     2161 ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa
     2221 ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc
     2281 tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc
     2341 tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc
     2401 agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct
     2461 acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct
     2521 tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg
     2581 atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct
     2641 tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct
     2701 acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa acgtgcacgc
     2761 agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa aagtgaaaat
     2821 agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct ggatattttt
     2881 cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata atcttcaatc
     2941 tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt ttcaaagaaa
     3001 attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat cggatggcta
     3061 cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat
     3121 catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa atgacggcga
     3181 tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg aataattgct
     3241 gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc atgatctgtt
     3301 tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca cctttcagac
     3361 caataaataa aacggcaaat acagccatca tccataaagc aggactgttg actaagctaa
     3421 aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta cgaggatgtt
     3481 tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa agtccagtat
     3541 tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac
     3601 gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca tcatacctat
     3661 ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa tctaaaatac
     3721 acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag atctttcagt
     3781 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggagtagcaa
     3841 tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg
     3901 aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac tcttcttctt
     3961 ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca tctgcatcta
     4021 caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc
     4081 cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg gaaagatctc
     4141 cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact gtttttagtt
     4201 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
     4261 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
     4321 tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta aaaatgatca
     4381 atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat tccatatgtt
     4441 cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
     4501 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
     4561 gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca gctgtatctt
     4621 catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata tctgactgaa
     4681 gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta cgatcatttt
     4741 cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt acagtcaaat
     4801 ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg ttcaccattt
     4861 caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct gggaataagt
     4921 aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac agttctggga
     4981 atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa aattctttct
     5041 tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg tacccttctg
     5101 gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct tcttgcaact
     5161 gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca gcttgaaagc
     5221 cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta ataatattgt
     5281 ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc tcgacttgga
     5341 ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa cgatacacgg
     5401 taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa atgatcttcc
     5461 tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
     5521 ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct gacaaaacaa
     5581 ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatatttg aattggcgtg
     5641 aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt aaatccatta ctataagatt
     5701 aaaataaatg ttatcaagat acaagctatt ttagctttat tctttctgta aaaaagaaag
     5761 gagactaaat aaactattat gaaaaaagga aaacatgcta aaaaaagaaa atattcattt
     5821 ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac tattcttgga
     5881 tggcaatttt acaataatga acactctcag atagttgccc atgctcaatc agctcaaaaa
     5941 aagttgatac tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca actattaaga
     6001 aaaaatgatt atattggctc aatttacgta aagaaaaata atcgcatcat catggaaaaa
     6061 ggctatggct atgcgaataa actcacgcaa caaccaaatg atccttctct ttattatcag
     6121 attggttcgg ttcaaaaagc attgactact cttttgattt taaaacaagt cgagcgtggg
     6181 cacctttctt tagatactaa gctggctcaa ttctatccac aaattgctgg cagccagcaa
     6241 attactatcc gtgacttgct gtatatgcgc tcaggcttgc accgaactgc ttctccaacc
     6301 ataccgatga ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa atttacagat
     6361 tatcagacct ctcattatga ttcattgaac tttaccatat tgactggaat tttgatcaaa
     6421 ttaacaaatc aaccgtatga aaaattattg accgatgata tcatcgaacc tttaggatta
     6481 cagcatacaa aattttatga ccaagtcaaa aattcatcca atcatgcact atcctatcaa
     6541 atgtctaaag aggatgatta ctttaaacca ctgacagaat ctgaaacaga gatccataac
     6601 gaattaggga ctggtaatat cagtatgtcc gtctatgatt taaatacgct gtttacacaa
     6661 gttttatctg gaaaactagt ttccaaagac ttactcttct cattatggcg tgaaaatgcg
     6721 aatggacatc catatagtgg tggggtatat agcggagata attatatcct gtcgcaagga
     6781 aatatcgact cttttgatac atctgctata tttagcaaag attcacagga tgctatcatc
     6841 atggaaagca atataaaagc tgacaaaact atcaaactgc ctactgctga tctaagaaac
     6901 caaatctatg acctgatgga aggaactact aagttggcaa atagctgata tgatgaaaag
     6961 atagcaaata ctacttcttt gaatagcatt tgcttaaaaa aagtactcga ctaaacgtag
     7021 gaaagagtgt gggacaaatc taaaaatcaa tttttgctac agactttaat tccaaataaa
     7081 cagaacagaa agaggtgttc ttctttctgt tctgtccaaa ttattctaag atttctcttt
     7141 accaacatta tttgacaaag ggcctattaa atctaaaata gtatctttag attgctaaaa
     7201 tatgtgctag caaatcattc atggcttgtt cttattttta attttatgca ataaaaaaga
     7261 agaacaatta gcgtgattat agaaataaat aatgagattt tagaaaaaaa tgtgtatgaa
     7321 tacctgattg ttactttatt tattccactt tcacttttta ctgtaacaaa aggacctcct
     7381 tgttttagta ctgtagcatt ttttgaatta acagtaaccg ttaatccttt ataccggaat
     7441 acaggtaaaa ctattgtttt gccttttgaa gatttaagcg taaatgtaat ttcatcttta
     7501 tttggagaaa agctactata aataacttga ccatcttctg taacttgatg gtttttcaaa
     7561 agcaattgat cttttttata gcctataggt acataatcaa aattctcaaa ttcttcaagt
     7621 ttattttcca tttgttcatt agaataagtt ccttgaaaat tcatttcttt caccatgtta
     7681 actactgatg aataatttaa aattgaaata gctaatatta ataatagtat aaaatttctt
     7741 ctccttgtat aagaaaattt ttccaactgt ggagaaataa atagagatac tgaaaaagat
     7801 atgaacaatg ttgaaaatat cataaaacgc caaggagctt gaataatatt taaaggagta
     7861 ttttgtaagg caaaccatgg aaatattttt gtacaaagta gaatagatat tatacccatt
     7921 atataagtga ttttagataa gattcctaat tttttaaagt taaatgttag tataattaaa
     7981 caaataagca aagttaagcc aataccaaat gatagtaatt ctgaatctat acttccaatc
     8041 aaaaactctt tacccagata aattgctctt ttttgtaata tatccacata aggtctattc
     8101 agatcttgat acaatgtctg ctcaatcata ggacctataa acgctagtga aagaagtaaa
     8161 gtaactatag cagcttttat taatttaata attaagaaga tttctatttt ctttttccga
     8221 taaaattgaa taattaaagc aattataatc attaccatag tcatagctac agaaagaaca
     8281 tgagtgtaaa ttaataaagt catgctaatc gccaacataa cccaagattc tttttttgag
     8341 tcgtaaagct ttataaaccc gagtaaaata agaggaagta ttgacattgc gagtaattcc
     8401 cccattgccc ctcgaagaaa tatttctaca ctcctaaaag aagatgttgt atatataata
     8461 gcgaaaaagg agctagtaac atgttttttg gtaatttcat acattacata atgacatata
     8521 aatagagtaa tgagcgtaac aatataaaga aaaaggttat agcctattgc taaattagta
     8581 aacttgatca ataaaaataa aggatataga gttaaccatg ggtaaaaatt atttacttgc
     8641 atgccatatg atctaaacgt ttcaaaatta ataggggagc taaaaataga ttctaaactt
     8701 aacgcacgat taatgtgaaa tgccgtatca taaaagccaa atatttccat gtttttaaat
     8761 gctggaaata aatataataa a
//
LOCUS       DAFBQW010000098         8722 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFBQW010000098 DAFBQW010000000
VERSION     DAFBQW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.101"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            7..2199
                     /locus_tag="LK889_002701"
     CDS             7..2199
                     /locus_tag="LK889_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBK5096886.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="LK889_002702"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="LK889_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK5096887.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2676..3071
                     /locus_tag="LK889_002703"
     CDS             2676..3071
                     /locus_tag="LK889_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBK5096888.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            3086..3985
                     /gene="era"
                     /locus_tag="LK889_002704"
     CDS             3086..3985
                     /gene="era"
                     /locus_tag="LK889_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK5096889.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="LK889_002705"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="LK889_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK5096890.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            5324..6241
                     /gene="glyQ"
                     /locus_tag="LK889_002706"
     CDS             5324..6241
                     /gene="glyQ"
                     /locus_tag="LK889_002706"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK5096891.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            6243..8318
                     /locus_tag="LK889_002707"
     CDS             6243..8318
                     /locus_tag="LK889_002707"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK5096892.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2943 a         1560 c         1853 g         2366 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaagaaa aacttggaaa ggcagcattg
       61 cctttcctgc tgttcttgtt ttttatcatt atctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa
      181 acgatcgaga acacggaaga aaccgaacaa aaaagaaaac tggcagcaga agcagtcact
      241 ccagaataca cttaccaaaa agatcttgca gatgaccaga acaatcggat caagcaacta
      301 tttgaattga tcgataaaac gaatgatgcc atcaacaaat cttatgacga aaagattgat
      361 aaggcaaagg aagatgaaaa tatcccaaag cctactacag aagagcggat cgccgcgttg
      421 aagaaaaatt ttgaaaatgt gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaactgaga tccatactgt gagggatgag
      541 agtctgaagc tcattgacga acagatgagc aaacaagttc gtgaaagtga attggaagct
      601 tttaaacaag aagcagaaga gcaaatccaa tatttgaatg tcacaccagc acaacaacaa
      661 atgattcgct acttagtgga tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag
      721 acagaagaat tgaaacaatc cgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaatcattg ttcgtgaagg taatcaaatc gatgcttcag ctatgaaaaa attggaatta
      841 ctaggactga ctaatcaaac aacttcgatt tttccgctaa ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggcgtact gatttataat tcacttcaat tccaaaatgc aggagaaaga
      961 acaaaatacg tgcttttcta tgtgacagct atgtcagtca gcatagtgct gatgaaattt
     1021 ttccaattat tccaaacgga acaagctact tatatcccgc tgttttatcc cgctgctttt
     1081 gcaccacttg ttttaagctt tttcttgaac cgaagagcag ggatcatggc tggtattttc
     1141 caagcagtat ctgcactttt cattttttat gattcgattg gcacgaactt tctaacgatt
     1201 atcttgatgt cttatgtttt ctctgggctc atggcaacga ttgtcagacg caaacggatc
     1261 tcagaacaag gattatctgc tgccatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatcg tctatcaagg aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc
     1381 tgcgggttca ctggtgcact cttctcattc ttgctgacga tcggtctaca cccatatatt
     1441 gaattgatgg tcaatgatga cagtgtaatt gtattaaatg aactcagcaa tccaaatcat
     1501 cctttattga aacagttgct ggaagaagcg ccaggtactt atcatcatag tatgatggta
     1561 gctaatctaa gtgctaacgc tgtagcagaa atcggcggac gttctttgct aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacatgcta gctttttcgt tgagaacttg
     1681 ccttcaggag cggaaaaccc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg agccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcaaacagca tcatggaacc acgctaatgc gttatttcta catcaaagcg
     1861 aaagaacgca atccggaagt aacagaagag caattccgtt atcctggacc aaaaccgcaa
     1921 agtcgtgaag cagggatcgt caatattgct gacagttgtg aggctgcagt acgggcaatg
     1981 gatcatccga cgagcgaaaa aatcgaaagt tttgtccata atcttatcga agagcgtatt
     2041 tccgatggtc aattagacga tagtggactg accttgaaag aaatccgaaa agtggaaaaa
     2101 tcattgatca gcggtttaag tagcaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 acagaagttg agaagataaa ggaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattaccttt atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgatga
     2281 cttgctgcaa tttgcagcat cttatttaaa gctgccagag gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcggcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tgcctcgtgt actaggagac atcatgattt ctattgaacg
     2521 tgctgctgaa caagcagaag cttacggcca ttcatatgat cgggaacttg gctttttagc
     2581 ggtacatgga ttcttgcata tcaacgggta tgaccatatg acaccagaag atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttgga tgcctatgga ctcaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaatatg aggaaacatg ttgcgttcgg tatccttgct ttatttatgg
     2821 gcgtgatttt ccagcttgat cggatagagt ggttatggct gcttttagct gtttttctcg
     2881 tttggatagt agagatcatc aatactgttt ttgaaaatgt agtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt tgctgtcatc attgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaaccga atcgtgggtc aaaaaattgc
     3181 cattatgagt gacaaggcac agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ctgattttta tcgatacacc aggaatccat aaaccaaagc atcgtctagg
     3301 agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggatgcag tcttatttat
     3361 gatcagtgct gatcaaaaac gcggccgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct ccagtttatc tgattatcaa taaaatcgat aagatccatc cagacgagtt
     3481 gctgggaatc atcgaagatt attcgacgca gatgcctttt gcacaagtga ttccgatctc
     3541 tgctacagaa ggaaacaatg tcgaacgctt aatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact ttatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggccacgat
     3781 catcgtcgaa agagatagcc aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa
     3841 gcaagtagga acgaaagcaa gaaaggatat cgaaaatctt ttaggagaca aagtatttct
     3901 agaactttgg gtcaaagtcc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 tggctaccga aaagacgaat actgatattc tgaacttctt ataggttaag tatagaaata
     4021 aactggataa aaatccgcac gttgagaaaa acggcggaag atcgaaacat aaataatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcgaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaaggaatc catcggcaaa
     4261 atcagccatt agctccggct ttgctcccct ttaccgaagc agtctatatc ggtaattttc
     4321 gaagtgaagg tttgtcgttt ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc
     4381 aagaagatat ctttttgagc ggttatggta cctatttgct aaatctagta gatgcagcaa
     4441 tcgaagatca tcaatatgac ccgcatttat tccaatttac gcaacaagct ctgcagcgga
     4501 tggatcaagg agatgatgca gaaatcatca cgaatatctt tgaagttcaa atactgcaac
     4561 gcttcggaat cgcgccaatt tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatct ggcggtgtga tatgcgagaa acactggcca atggacgaac
     4681 accgttatca tgcagatcct cgcgctgttt atttcgttcg aatgttttcg gctatttctt
     4741 atgataagat cagtggaatc cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca
     4801 ttgaccagtt gtatgatgaa tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga cgccagagaa aaatatgcct gaaaaagaga
     4921 tacctgaaaa agaagaataa atgaaagata caacaaaaaa atcaaaacta tttgacaaat
     4981 acagggactt ccgtctatta ttaaccgtag attaaataga tcgttcactg agaggatcta
     5041 ttatataaac aagaaaactg caaaatgaat acgtacattg accaagatca gtaaaaaaca
     5101 cgtggtttag cgagttcggg agagtggaag ccggacccaa acgatttttg aagaggcact
     5161 tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa
     5221 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
     5281 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
     5341 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
     5401 gcaagcttat gacacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
     5461 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
     5521 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
     5581 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgtcttt taggaattga
     5641 tccattagaa cacgatattc gttttgttga agacaactgg gagaacccat cgatggggtg
     5701 tgccggtgtg ggttgggaag tatggcttga tgggatggag atcacacaat tcacgtactt
     5761 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagatcactt atggactaga
     5821 acgtcttgct tcatatattc aagaagttga aagtgtctat gatctggaat gggctgatgg
     5881 tgtgaaatat ggcgagatct tcaaacaacc agaatatgaa cattccaaat attcttttga
     5941 agtaagtgac caagacttgc tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg
     6001 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gcagccatac
     6061 attcaattta ttagatgctc gcggtgcagt atccgtaaca gaacgagcag gttatctttc
     6121 ccgtatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt
     6181 aggattccct ttattgaata aaaacacagc aaatccagaa aacatgaagg aggctgaata
     6241 agatggcaaa agacttatta cttgaaatcg ggctggaaga aatgcctgct catgttgtca
     6301 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     6361 cttatgatac gatcgaaacg ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca
     6421 ttccagaacg tcaagcagac agtgaagaag aagtcaaagg accagccaaa aaaattgctt
     6481 tggacgcaga tggaaactgg acgaaagcag ctgaaggatt tgtccgcgga caaggtctga
     6541 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     6601 acactcacgg caaagaagcg aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     6661 tcactttccc agtaacgatg cattgggcag aatacgattt tgaatatatc cgccctattc
     6721 actggatcgt ggcattgtta gatgacgaag taatcccgtt tgagatctta gatgtcaaaa
     6781 caggcagagc ctctcgagga catcgtttct taggcgaaga tgtgacattt gcgcatgcaa
     6841 aagaatatga agaaaaattg aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag
     6901 aaatgatcgt tacacaagca aatgaattag ccgaaaaaaa tcactgggtt attgatctag
     6961 acgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc ttttctggcg
     7021 acttcgaaga aaaatatctt tctgttcctg aagaagtact agtcacttct atgaaagaac
     7081 atcaacgtta tttcgaggta cggaacgatc aaggtatgct gcttcctcat tttatttctg
     7141 ctcgtaatgg tgatcgggta aaattggaaa atgtggttcg aggcaatcaa aaagtattga
     7201 cggctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     7261 aatacgttga aaaactaaaa actgttactt tccatgaaaa aatcggtagt atctatgaga
     7321 aaatgcagcg ggtacaagtc attgcccgta tcattggcga acaagtagga ctatctgagg
     7381 aagaattgat ggatctgcaa cgagctgctg aaatctataa atttgatcta gtaaccaaca
     7441 tggtagggga atttcctgaa ctacaaggga tcatgggcga aaaatatgct ctgctgaaag
     7501 gcgagaaacc tgctgtagca acagcgattc gtgagcatta catgcctata tccagtgaag
     7561 gagagctacc tcagtctaca gtaggttctg ttttagcgat tgcagataaa ttagatagca
     7621 ttttttcttt ctttgcagtt gggatgattc caagcgggtc aaacgatcct tatgccttac
     7681 gtcgccaagc atacggtgtc gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt
     7741 ctgttctgca agaaacgatt agtgatgtta tcagtaaaga tacggatcgt ttcggcattg
     7801 gtttatcaga agggcaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     7861 tgacaacgaa gaatattcgt catgatgtca tcgaagctgt tttgaatgca gaacaaaaag
     7921 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     7981 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     8041 aaaaacaatc agaagtagac cctgaactgt tcgaaaatga agcggagaaa gaactgcaca
     8101 aagcagtaga ggcaatgaaa aaaagctttg ctgaccaaac gatcaacgaa aattatgatc
     8161 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     8221 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     8281 tgtcgctagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     8341 gagaataggc acaaaatagg ctatttctct ggtttatttg ttataaatat aaataactat
     8401 acccgtggtg ctagttaatt ttttcctagg tattatacaa gtaatttgag tcggtcaatt
     8461 tcaactgaat tatctaaact aactgatcta cttttagata attcagttga aatttattgg
     8521 taaactgtcg cttttctttt taaatggcaa aaatttttaa taatatatga caatttagaa
     8581 atcaactata agactgatta attcaaaatg atcttagaat cattgtatga acactaaata
     8641 ctatattgtt ttctattaat cgttatataa acgattatga attcaaagct actttttatg
     8701 acgtcaatca gagagtgtaa aa
//
LOCUS       DAFBQW010000099         8493 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFBQW010000099 DAFBQW010000000
VERSION     DAFBQW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.102"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..173)
                     /locus_tag="LK889_002708"
     CDS             complement(<1..173)
                     /locus_tag="LK889_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5096893.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LK889_002709"
     CDS             complement(593..1714)
                     /locus_tag="LK889_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBK5096894.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LK889_002710"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LK889_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBK5096895.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LK889_002711"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LK889_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBK5096896.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LK889_002712"
     CDS             complement(4304..5215)
                     /locus_tag="LK889_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5096897.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LK889_002713"
     CDS             complement(5232..6239)
                     /locus_tag="LK889_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK5096898.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LK889_002714"
     CDS             complement(6236..8365)
                     /locus_tag="LK889_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5096899.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="LK889_002715"
     CDS             complement(8370..>8493)
                     /locus_tag="LK889_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5096900.1"
                     /translation="TGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1487 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg gtt
//
LOCUS       DAFBQW010000100         7930 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFBQW010000100 DAFBQW010000000
VERSION     DAFBQW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.104"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            143..847
                     /locus_tag="LK889_002716"
     CDS             143..847
                     /locus_tag="LK889_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5096901.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1075..1413
                     /locus_tag="LK889_002717"
     CDS             1075..1413
                     /locus_tag="LK889_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096902.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            1398..2252
                     /locus_tag="LK889_002718"
     CDS             1398..2252
                     /locus_tag="LK889_002718"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK5096903.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            2399..2632
                     /locus_tag="LK889_002719"
     CDS             2399..2632
                     /locus_tag="LK889_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBK5096904.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            2830..4734
                     /locus_tag="LK889_002720"
     CDS             2830..4734
                     /locus_tag="LK889_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK5096905.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            5088..5453
                     /locus_tag="LK889_002721"
     CDS             5088..5453
                     /locus_tag="LK889_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5096906.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            5470..6081
                     /locus_tag="LK889_002722"
     CDS             5470..6081
                     /locus_tag="LK889_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HBK5096907.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            6109..7770
                     /locus_tag="LK889_002723"
     CDS             6109..7770
                     /locus_tag="LK889_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBK5096908.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            7798..>7930
                     /locus_tag="LK889_002724"
     CDS             7798..>7930
                     /locus_tag="LK889_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK5096909.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     "
BASE COUNT         2506 a         1294 c         1766 g         2364 t
ORIGIN      
        1 taaaagtaga agctgattta gggattggaa ttctttccag tcctttttcc attttcttga
       61 tgtgcatcaa tttttttgta gtaagagact tatataataa aagcagcgaa tataaacaaa
      121 ataaataaag ggggagaatg aattgactaa acatacccat catgaaattg gcgaccacgc
      181 agcctgtatc cgcttggttc caatattcaa tcacttagag gatagcgcga tgaattacat
      241 tgctgaaaaa gcccacacga aacagtatca aaaaggagag tttctgtttc gttcacaaga
      301 aaaagaggat actttatata ttatcaatcg tgggaaaatc cgtatttatc gattggcaga
      361 ctctggcaaa gagcagctag tgcggatctt gaaccctggt gatttcaccg gagaatggac
      421 cttgttcaac ccggatgcag tacacgagga atatgccgaa gcaacccgag ataccgttgt
      481 ctgtatgatt cagcaaaaag atgtccaaga ttttctggaa caatacccag ctatttcttt
      541 aaaactattg gcggaaatgt ctaaacggtt agaaagttca gaacgtcaaa cgacacaagt
      601 agccgtggag agtgtcatta cccgtttagt tttgtttttg gcagaaaatg tggaacctga
      661 aatgggcaac tctcccacca tcaccctgcc gatggcaaaa aaggacattg cttcctattt
      721 aggaacgaca cctgagacca tcagtcgcaa atttggagaa ttggaggacg aaggattgat
      781 tcaacagctg tctgggaaaa ggatcaagat tcaggattta gatgatttat tgctttacag
      841 cgaataatcg gacacacttt tttctacacg ttggtgttct gtcgctcttc attaacaacg
      901 ggacagaact cagtgctttt gcttccatct gtccgttttt ggctttttta agacagaacg
      961 accttcattc attggaaaaa taaaagcaca aacatgataa ccctcaagtt tttttgtttt
     1021 atttttcttt aagatagggt tgtcgaacaa aacgtaaaaa taagtagggt gaatgtgcat
     1081 tgtttttggg tgatagttgc catcacgttg tcagcagcgg gggcattctt tgtcgtccct
     1141 ttcaccaaga acacgattgg cggcgaagca aaaacgtcta acgttgttga ggcagatagg
     1201 ttagctatgg aaaaagaaga ggaagttgta aaaggagccg gcgtaatcgg acacggaaaa
     1261 gtggcgtatt acgaagcgag tgaccaggga gataaaagtg tattcactgt ttggtttcca
     1321 tggagcttag ccggaatctt gttcagcatc accatgacta gaatgacgcg aaaaattgaa
     1381 gaaatggagc aaatagaatg ggattcgtat taggcgtttt gaatccccat gtggtggaga
     1441 ttggttctgt tgtgattcat tggtatggag tcattattgc agcgggcata ttagcggctc
     1501 tccagttgag caccaaggaa gcaaagaaaa aaggactgga agaggatacc attatcgata
     1561 tggccttatg ggcaattccg attggactcc tcggcgctcg gctttattat gtgttatttg
     1621 aacttggtta ttatctgcaa aatccaggac aaatccttgc gatttggaat ggcggcattg
     1681 cgatttatgg gggattgatt gcaggagggg gtacgctata ttggtatgca aagaaaaaag
     1741 gtacttcttt ggcactcgtt cttgatattt tagcgcccaa tgtattgtta gcccagtcca
     1801 ttggtcgttg gggcaatttt atgaatcagg aagctcatgg tgggccggtt actcgtcaat
     1861 tcttggaaaa tctttattta cctgaattta tcattgaaca aatgaatatg aatggtacct
     1921 actaccatcc gactttttta tacgagtcat tgtggagttt gttgggcttt gtcctcatcg
     1981 tcactatacg gaaccgaaaa cagcttttgc gtcaaggaga agtcgctttg agttacgttt
     2041 tgtggtactc agtgggtcgg ttcttcattg aaggtatgcg aacggacagt ttatggattg
     2101 gcgagtggat ccgcgtttcg caagccttgt ctctggccct gtttattggc gcgattgtag
     2161 tatggtttat acgcagacaa gattatccac caatttctta ttattctgat ggcaataagg
     2221 ggcagaaaaa gactattaag atggtgaatt gagcaaaaag ataaaaagaa gtgcaaaact
     2281 tgatatgcgt caattttcgt ttgctaaaag tgagctatac tacagttgta aagaggaaaa
     2341 ggcagtgttc attaatctgc tgccgataaa ataaaaacac ataaaggaga taggaattat
     2401 ggaaaaagct atattgcaat tagaaacgtt gtcttgtcca agttgtatgc aaaaaattga
     2461 aggtgccgtg aaatcagtta acggtattga taaagacagc attaaagtat tattcaactc
     2521 cagcaaagtc aaaaccaatt ttgattcagc tgtcacttcg attgaagaga ttcaaaaagc
     2581 tatcgaaaac gtaggctatc cggttcttaa agcaaaagtc aaggccgttt aacagattaa
     2641 atgaactagg caattctgga agaaaagcta tttttccagg attgcttttt ttgttgcaat
     2701 ccgattcgag gtgcttagaa acgataaaag agaactttct aaacttgatg tcagtcaatt
     2761 aattacttag gcaattatct tatactgtgt ttatcaataa aagagaaatt aaatttggag
     2821 ggaaaaagca tgcaacaata tatattaagc aagaaaaatg ttatcaccgt catcagtgga
     2881 ttgttaatcg tactcggttt cttcagtcac tttgttttag aaaacgtagg actttcagag
     2941 tggtctttga tcatcgcctc tgtctttggg attacgccaa ttgccattca agcgtttcaa
     3001 gcaatgaaag tcaaagtcat cagtattgat gtcttagtca gtattgcagc gattggtgcg
     3061 ctttttattc aaaattatga agaatcggct attgtcacgt tcttattctt attcggacac
     3121 tacttggaac aacgaacatt gaaccaaacg cgatctgcta tcaaagaatt gactgaaatg
     3181 gcacccgaaa gcgccttgaa acaaatggat aatggcgaat ttgaagaagt agaagtggat
     3241 gatatagatg aaggggatat cttgctcgtt aaaacgggtg cgaaagttcc agtagatggc
     3301 atagtcctta cgggtgaagg gcatatcaat gaagctagca ttacaggtga agctattccg
     3361 gtcaacaagc tagctgatgc agaagttttt gcaggaacca ttttagaaaa cggaacgatt
     3421 caaatcagag ctgaccgagt tggtgaggac accacatttg gaaaaattat tgaactcgtg
     3481 gaagaagcac aagattctaa atccgaagcg gaacggttca ttgatcgctt ttctaaatac
     3541 tacacaccag ctgtcttggt tctggcaatt gttgtatggg cgttcagtca aaatattgag
     3601 ttagcaatca ccatcttggt tctaggttgc ccaggcgcat tagttatcgg agtgcctgtc
     3661 tcaaacgttg ccggtattgg gaatggtgct cgtaacggtg ttcttttaaa agggagtgaa
     3721 gtcattcaag acttcagtaa tgtggataca attgtatttg ataagacagg tactttaact
     3781 gtcggaaacc caaccgttgc agcgactgaa ctgtatgaaa aagatcctgc tgaaacgctt
     3841 ggctatctgg ccagtgtgga acgggaatca gatcatccat tagcaaaagc ggtcttaaat
     3901 caaattggag aaacaaactt ttatcctgtt gaaggaactg aagttgtgaa aggcggcgga
     3961 atcgtttcaa gtgtagctgg acatagagtg gctgttggga atgtggcctt gatggaaaaa
     4021 gaaaatgtca ctctcagcaa aaaagtgcaa aaagatgtca aacggtttga acaacaaggg
     4081 aactctctcg ttttgacagc ggtcgacggt gaattaaaag tactgatggg cattcgcgat
     4141 caagtccgtc cgggtgtgaa agctaatttg caggaattaa aagacttggg cgtgaaaaat
     4201 ctagtcgttc tttcaggaga taaccaagga accgttgatg tggtcgccgg cgaacttggt
     4261 ttgaccgaag ctcacggcta catgttgccg gaagacaaat cggcttatat cggaaaactt
     4321 caacaacgtg gtcaaattgt agcctttgtt ggagatggcg ttaacgatag tccgtcctta
     4381 gctttagcag acattggaat cgcaatggga agcggaacgg acgtagcgat tgaaacatcc
     4441 gatgtcgttt taatgaactc gaacttcagc aacttgcctc acgcattagg attagtaaaa
     4501 gccaccgcaa ataatatgaa acaaaatatc gtgatttcag ttggagtagt gttggtcttg
     4561 ttgaccagcg tcttcttcag cgaatggatg aatatgtcta tcggaatgtt ggttcatgaa
     4621 ggtagtatct tggtggtaat tttcaatggc atgagattaa tgaagtataa gttgaaaggt
     4681 cggaaagaac aaaaagaagt atcagcacct acagagaaag taaaaatatc ttaagaataa
     4741 attagaagag gctgagacaa tagtccatcc ttgaaatgac aaaagcggaa actcagattt
     4801 ttaaatctgg gattttccgc ttttttgagt tcttgaaaaa aaataagagt atagagtatg
     4861 ttctttataa aattaatttt cgtgtaaatt attaaaaatt taataataaa atttaaattc
     4921 ctattaagtt gtaacacatg aaattgctta aaatagtgta tcaaaaacct tgttttatta
     4981 atgttgaatt tatttgaaag gtctacagat taacatatta ttgacaaaac aatatttaaa
     5041 tagttatact tcattcaaac gtctgtttga acgaggtggt gaagttaatg actgcagaag
     5101 tatgtacaac tacttgtata cataaagaaa aagtagctag tgttaaaaat aatttaaaag
     5161 ataaagacct ttcaacttta cttgtattag gaaaatgttt tagcgattct tctaggatta
     5221 aaattttcta tgctttagaa acgtataagg agatgtgcgt ttgtgattta gcagcgatac
     5281 ttaatgctag tattgctaca acgtcacacc atttacgttt tttgaaaaag aacggaatgg
     5341 cgaaatcacg tcaatatgga aaggtagttt attactcttt agcgaatgaa gagattcttt
     5401 ccgctgtgca ggcttttcta aagttgtcag aaaatagttc tatgaaagct tagtttggag
     5461 gtagttattt tgttacaaaa cattttatca gcactcgctg tatatatttc taccagtatt
     5521 gattatttat ttatcttgct gattattttt tctcaaaacc atactaaaaa aggtctccgt
     5581 cagatttttt ttggtcaata tcttggaaca ggaatcttgg tagccataag tttgtttgca
     5641 gcatatgtgc taaattttat tcctcaagat tggatcattg gtcttctggg actaattccg
     5701 atttatttag ggattcgtgt tgcttttgtg ggcgaagagg aggaagaaga aggggaagtc
     5761 gttgagaagc taggatctag aggaacgaat cgtttgtttt ggacagtttc cttaataacg
     5821 attgcttctg gtggcgataa tttagggatt tatattcctt tctttacctc gttatctttt
     5881 tcagaaattg tcacttcttt aatcgtcttt gctatctctg ttgcggttct ttgctatatt
     5941 agttataaat ttgcgaaaat ttcttttgtc tctgaaacac tagaaaaata tgaacgaatc
     6001 attgtaccta tcgtatttat tggattaggg atttttatta tgatagaaaa tggaaccatc
     6061 caaactatat taggtttgta gagatcttat tagtaaagga agcaaacgat gagtgaaaat
     6121 aaaaagtacc atgaatggat attagaagga atcgactgtg caaattgtgc agacaaagta
     6181 gaaagaggcg tcgccaaagt tgcaggtgtt gccaatagta gtgtgaacta tatgacagaa
     6241 accttgagtt tcgaagtgac agaggaaaaa gaatatcttg ttttatccaa tgtgcaacaa
     6301 aaagtaaaaa aattggagcc tgatatcact ttaaaaaata aagtggacgg taggctgatt
     6361 gaaataaatc cggcattaat caagaaaagt aatttaaaag acgataaaaa aaactacgaa
     6421 tggatattag aaggaatcga ttgtgcaaac tgtgctgcca aagtggaaag aggagttgct
     6481 acagttcccg gcgttacaaa cagcagcgtc aattttatga cagagacact gagcttcgag
     6541 gtagcaggtg aaaataaaac tgacgtgttg gctagtgtaa aacaaacagt aggaacttta
     6601 gaacctgacg ttgttctgcg aaaaaaatcg gacggtacat tgattgaaca tggaactgac
     6661 tctaataaaa attcgacttc taacaaatcg atttctgata aagatgagac aaaccaaaag
     6721 cagacgaata gagcttatct ggccagcctt ctcggaaata aaactaaaat tacccttatt
     6781 cgtttaatac taggattagg aatattagcg acaggaatat ttctccctat ggatggacaa
     6841 gcctctcttg ggttgttcat tctggcttat ttaatcaccg gttatgacgt tgtaggacgt
     6901 gccattagaa atattttccg aggtcaagta ttcgacgaga actttctaat gaccattgcg
     6961 acgttaagtg ccttctatat tcaagagtat cccgaagctg tagcagtcat gctattttat
     7021 caagtgggag aagtattcca agatattgct atttcaaaat ctcggcgctc cattgcggac
     7081 ctaatggata ttcgacctga ttatgccaat ttagtcacag gaaacaacgt agaaaaagtt
     7141 gctcctgaag caattaagat tggaaatatt attctcattc gtccgggcga aaaagtgcca
     7201 ttggatggaa aagtagtgga aggtgcttct gcgatggaca cttctgcttt aacaggcgaa
     7261 tccgttccaa gaagcgtgaa gccggaagac actattttga gcggatttat taataaaaac
     7321 ggattgctaa aagtagttgt agaaaagccc ttttcggagt ctactgtgaa gaaaatttta
     7381 gatttggtcc aaaatgctag tggccggaaa gcacctactg aacaattcat taccaaattt
     7441 tctcgctact atactccaat tgtggttata gctgctgcct tactggcaat tgttccgcca
     7501 ttgctagtac caggcgctac ttttaatgaa tggatatata gggccagtat ctttttagtt
     7561 atttcatgcc cttgtgcttt agtcgtttcg attccagttg gtttctttgg tgggattggt
     7621 tctgcttccc gcaaggggat tttagtaaaa gaaagcaatt tcctcgaggg tttgaacgat
     7681 cttaaatacg tggtaatgga caaaacgggt acattgacaa aagggttctg ttgcaaagtt
     7741 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagtaggagg aatgacgatg
     7801 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggatactat
     7861 cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg tggcattaac
     7921 gtttctcata
//
LOCUS       DAFBQW010000101         7873 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFBQW010000101 DAFBQW010000000
VERSION     DAFBQW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.105"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            170..514
                     /locus_tag="LK889_002725"
     CDS             170..514
                     /locus_tag="LK889_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK5096910.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="LK889_002726"
     CDS             515..934
                     /locus_tag="LK889_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBK5096911.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="LK889_002727"
     CDS             complement(1026..1376)
                     /locus_tag="LK889_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096912.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="LK889_002728"
     CDS             complement(1342..2673)
                     /locus_tag="LK889_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBK5096913.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="LK889_002729"
     CDS             2929..3495
                     /locus_tag="LK889_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5096914.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="LK889_002730"
     tRNA            3640..3727
                     /locus_tag="LK889_002730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            complement(3779..4984)
                     /locus_tag="LK889_002731"
     CDS             complement(3779..4984)
                     /locus_tag="LK889_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5096915.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLFFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5122..6009)
                     /locus_tag="LK889_002732"
     CDS             complement(5122..6009)
                     /locus_tag="LK889_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5096916.1"
                     /translation="MKKMIGSLLVLASLLLFTACGKKVTTDDLKANDWLIEATKDDEP
                     NMIASFSDHIVSLSVDTSKMKSTASDEWDAMGEEFAKSLVDQMNYKFEYTLEGNVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKSNKSDDKEKLVLKPYKKEKTKDSRTTTSSTS
                     SNEQSSSTTTESTIASSSTVEQAAIAQNTPPETSSTYSAESVENYAEATPSSSVEQVS
                     STPSSTEQSVPQDYVPANQAQPTAPSSSETETLTLQPGEGPKQIAERAGITVDQLFAL
                     NGMDPNNYMLYPGQELRVK"
     gene            complement(6096..6296)
                     /locus_tag="LK889_002733"
     CDS             complement(6096..6296)
                     /locus_tag="LK889_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096917.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6310..6723)
                     /locus_tag="LK889_002734"
     CDS             complement(6310..6723)
                     /locus_tag="LK889_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK5096918.1"
                     /translation="MDNIEDLLKENGIDVEITNIESEGFYLPKLRTIFINQNLDELEQ
                     KKVLLHESRHALSHNELISLYSKTVFHSKMEDEANRFMIEVLLQDYMNLFALSVDQIN
                     YMKFMDYYGIGYDCEEYIKKLLVNYVTSSMYLNVI"
     gene            complement(6735..7073)
                     /locus_tag="LK889_002735"
     CDS             complement(6735..7073)
                     /locus_tag="LK889_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5096919.1"
                     /translation="MTVFERIKFLAKKHSKTMKQVTIDLGYSENYFYSLKSGKQPSAE
                     KLTEIANYFNVSVDYLLGRTDNSEDSDSQLPPTDLDDALDGAMTWNGKPLTDQDKEAV
                     RIFLQGRKSE"
     gene            7367..7558
                     /locus_tag="LK889_002736"
     CDS             7367..7558
                     /locus_tag="LK889_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096920.1"
                     /translation="MSNIDNGREAIKGFMKANNISEYDLATAYGRSRTWIQRVLSGKD
                     KGPAVNAFILEVIRDHKIR"
     gene            7569..7835
                     /locus_tag="LK889_002737"
     CDS             7569..7835
                     /locus_tag="LK889_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096921.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
BASE COUNT         2589 a         1335 c         1424 g         2525 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atatcaacga ttagacacga tttcgatcgt gtcttttttt gttgtaattt
     3781 agcctaaaat gttttttgac tccccttttg actccccctg agtactattc ttcataaatg
     3841 ctacaaaatt attgatgact tttttgtttt gagtttcagt tacatgagtg taaatatttg
     3901 aagtagtttg aatgtctgca tgccctaaac gttcctgaac atcttttaaa gaagctcctg
     3961 attcaaaaag gagagaggca tgagtatgtc gaaaaccatg agtagtgatt cttttagtta
     4021 attctggatg atgcttatat atgacattaa gccacgaacg tgaaactgta gggttataga
     4081 gaagaccatc atggtgaaaa aataagtttt cgctttggat tgtaacgatc gttttgtttt
     4141 ttgtataata ctttctcaat aaactgatca aatcattgtc cagataaatt tttctttttc
     4201 cagattttga tttaggagta tttacgatca atctcccttg ataacctctt gttacagttt
     4261 tattaatatt gagtgttttg ctcttaaaat caatatccga ccaggtcagt gcaaaagctt
     4321 ctccttttct tatacctgta aaagccaata gggaaaagaa taaatatctt tcatcatcat
     4381 cgtttcttat tgcttctaaa aattcgatta attcttcttt agtataaaat tcgatatttt
     4441 tatcttcgat atctagagct tctccacgtg gaatagaaac taatttcata ggattgctag
     4501 ttataatttg taaagaagca gcgtaatcaa aaacattaga tgtgtaattt tttattttct
     4561 taaaaatttt aggatggcta tcggaccaag tgtttactgc ttcttgacag aaaaaaacgt
     4621 ctattttgtc aatgaactta tctccaaaca cttttaagat gtgtgtgtca aaaatttgtt
     4681 tggtggagga ccaagtgctt tctttaactg tctttttata attttcaaac cataagccgt
     4741 aaacatattc gaatttcttg ctcttttgag ctgtagaagc ttgcagtcct ttttcctgta
     4801 tatctgcctc caatcttttt aaagctctct cagcggctaa tggcgtacta aaacctcgtc
     4861 tcgttgtttt tctttttttt cctgttagag gatcaactcc taagtacaga ctaaactgat
     4921 atttttcttc gccttttttt gttaagtgct ttttaattcg tttatcaatt tctttttttg
     4981 ccatctttat ctttcctttc gtacgtttgt tcggttgtac agcggatttc gagatggtaa
     5041 aatagggtac aacaaataga cctactttac cgtagctctt tgcacatttg cgttcttggt
     5101 cgggcggcaa atgtgctttt tttatttcac tcttagttct tgtcctggat aaagcatgta
     5161 gttgtttgga tccataccgt ttaatgcaaa cagttgatct accgtaattc cagctcgttc
     5221 agctatttgt ttgggccctt caccaggttg caaagtcaag gtttcggttt cagatgaaga
     5281 aggtgccgtt ggttgagctt gattagctgg tacataatct tgtggtactg attgctccgt
     5341 tgaactggga gtggatgaaa cttgttcaac acttgaacta ggagtagctt ctgcatagtt
     5401 ttctacactt tcagcagagt aagtactact agtttcagga ggcgtattct gcgcaatagc
     5461 agcttgttct acagtactag aagatgcaat agtactttct gttgtagtcg aagatgattg
     5521 ttcgttgcta cttgttgagc tagtagttgt tctagagtct ttagtttttt cctttttgta
     5581 gggttttaaa actaatttct ctttatcatc agatttatta gatttttctg gagtcaaaat
     5641 aatgtttttg tcttctttgc taacggtata cttggcatcg ttatccttgt ctttgtcgtc
     5701 tttccaagtc ataacattac cttcaagagt gtattcaaat ttataattca tttgatcaac
     5761 taggctttta gcaaattctt ctcccatggc gtcccactca tcgctagctg tagatttcat
     5821 tttgcttgtg tcaacactaa gagaaacgat atgatcagaa aatgatgcta tcatatttgg
     5881 ttcgtcatct ttggttgctt caatcaacca atcattagct tttaaatcat ctgtggttac
     5941 ttttttccca caagcagtga agagtagtag ggaagcaaga actaacagac ttcctatcat
     6001 ttttttcatt ttttattcct catttctatg atatgatttt tattggggaa tcttagaaat
     6061 aaggtttcga gtccgtgttg ctgcacgggc tttttttatt taaataaatc ccaaaaactg
     6121 aatgtagttt tcttgtatac tttattgtaa gcagcctttt ttggatcttt gatccaacca
     6181 gatccctttt taccgtaacc aggaatcact gcttttttca cagctctttt tgcctttcca
     6241 gtagttctag cgctaatgga tttttttata ctcggttttc tcattcctat tttcataaga
     6301 agcctccatt taaattacat taaggtacat agaagaagtg acatagttca caagtaattt
     6361 ttttatatat tcttcacaat cataaccaat tccataataa tccatgaatt tcatatagtt
     6421 gatttgatct acagataaag caaataaatt catataatct tgcaacagta cttcaatcat
     6481 gaatctattt gcttcatctt ccatttttga gtggaataca gttttgctgt aaagtgatat
     6541 caattcattg tgacttagtg catgacgtga ttcatgtagc aatacttttt tttgctctaa
     6601 ttcatctaaa ttttgattaa tgaaaattgt ccttaattta ggaagataaa atccttctga
     6661 ttcaatattg gtaatttcta catcaatccc attttccttc aacaaatctt caatgttatc
     6721 catacactac aacctcactc actttttcta ccttgtaaaa atatccttac cgcttcttta
     6781 tcttgatcgg taagtggttt tccgttccaa gtcattgctc catctaaagc atcatccaaa
     6841 tcggttggtg gtaattgaga atcactatcc tccgagttat cggttctacc tagtagataa
     6901 tcaacagata cattgaaata attagcaatt tcagtaagct tttcagcgga tggttgtttt
     6961 ccacttttta aactatagaa atagttttca ctgtatccta aatcaattgt tacttgtttc
     7021 attgtttttg aatgtttttt tgcaagaaat tttatccgct caaatactgt cataccagca
     7081 ttctcctttt ttctttacaa aaaaccaata aaaaagtgta gttttgtgtt gacctaaaca
     7141 acactatagt gtatattggt tttgtaagtt aattggatag aaaaaaagca aagtaaaaac
     7201 acaccttata gcattaagtt tggcgaccga gtgcgataaa aaaaggtttg ttataggctt
     7261 atttaactat ggctatatac tacactatag tatagttcgt agtcaactaa aaatatactt
     7321 ttctatccaa ttttctttct aaataaaaag aaaggaagtg tgtgaagtga gtaatatcga
     7381 taatgggcgg gaagccatca aaggatttat gaaagcaaat aatatttcag aatacgattt
     7441 ggccactgca tatggtagat cgagaacttg gattcagcgt gttttaagtg gaaaagataa
     7501 aggtccagct gttaacgcct ttattctgga agttattcgc gatcataaaa ttcgatagga
     7561 ggcaagtcat gaatattcta agcgaagagt ttctgactcg attgagaatc gcaattgttg
     7621 aagttgtaaa ggacgcactt agtcaacttt caaaaaagaa tttgtcagaa acacgatatt
     7681 taaaaaagat cgaagctaga aaatatgtgg gaggtgtaaa cgatcaaggc tttgagaagt
     7741 taatagctca cggtttaaaa gaaattcgta tagatggctt tttgagatat gacaaaaagg
     7801 acatcgatga actgatggct aaatacaaaa tttaaaggag gaaaaacatg ggaaagttta
     7861 atcgaacatt agt
//
LOCUS       DAFBQW010000102         7824 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFBQW010000102 DAFBQW010000000
VERSION     DAFBQW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.106"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1182)
                     /locus_tag="LK889_002738"
     CDS             complement(<1..1182)
                     /locus_tag="LK889_002738"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5096922.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
     gene            complement(1361..2134)
                     /locus_tag="LK889_002739"
                     /pseudo
     CDS             complement(1361..2134)
                     /locus_tag="LK889_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2155..3357
                     /locus_tag="LK889_002740"
     CDS             2155..3357
                     /locus_tag="LK889_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HBK5096923.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            4090..5058
                     /locus_tag="LK889_002741"
     CDS             4090..5058
                     /locus_tag="LK889_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5096924.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            5069..5884
                     /locus_tag="LK889_002742"
     CDS             5069..5884
                     /locus_tag="LK889_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBK5096925.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5952..6662
                     /locus_tag="LK889_002743"
     CDS             5952..6662
                     /locus_tag="LK889_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5096926.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVKRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6698..>7824
                     /locus_tag="LK889_002744"
     CDS             6698..>7824
                     /locus_tag="LK889_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK5096927.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINYFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         2600 a         1234 c         1589 g         2401 t
ORIGIN      
        1 atggagattt tccagtcgtg cattcgaata cttcttcagt aatgccagcc gaatcccttt
       61 ctcgtatttg aaaaaggctt ttttgatatg gtgcagttcc aatggaactt ctccaggcat
      121 tgctcgaagc agctggaaaa aagtatctgg atctttttcc tgatacgcag taagcaggtc
      181 ttggtagtaa cgataagctg ttttaagttc aggagaaatc tttagaaggc ggtcaactac
      241 ttctgtttca gtcaatagtc tccaattgaa attgcgtctt ttcgttagtg tattgtaatc
      301 taaacgatcc tctgatttta aaagtaattt gaataaggat ttcaattggc gatactgttt
      361 agcttgtatt gggtcttttt gatcaagtgt tttcatcaca cgttttcgaa ccgcatcgaa
      421 gaagcggttc agatgttgaa caatgtgaaa ccgatcgacg atcacttgtg catttggaaa
      481 acactcttta actagtgaga aataaggagc gttcatatcc gttacgatgt aatgtacacc
      541 caacctcgct ttgagagaaa aacgcatgaa aaaagcaacg agatcttttt tacggcggct
      601 aggtaagatc tcaaacagtt tcccggtctc accgtccata caagaaaaag aaaatttacc
      661 gtgccatgta gaaagagaac gaaactcatc gaataataga caccgaggta aatactcaag
      721 attctgacgg atatctgatg acaaggcgga caatgtccgc tgaacagttg tcacagaaac
      781 atcgcaatgt ttaccaattg cttttataga ttgacgctct gtcaattctt ctaagatctt
      841 gtaatgaatc tgcttagaaa taaagcagaa tggggtaacg agatacgtcc gagcggtaaa
      901 atatttttta catgctcggc agaggtatcg ctgtttcttc aaccgaagat aggtccgttt
      961 attaccgcta cgattcagga gaatcataga gatcttcttg ccatttttaa cgatttgctt
     1021 tgtagaatct ttacaacacg gacaacgtct gtaggtacgg atcagagttc cctcaataac
     1081 gatacttttt ctgcttttat aaaaagtttc ttcaaaggat tgaatttgga tatttgagtc
     1141 tttaatagta aagagttgtt tgataaaatg attcatggac atacaagaat tcccccagaa
     1201 tgatgttgta ggagactcta ttctaccgga aattttgtat gtctttttgt ttttttacaa
     1261 aaaaactagg acagatagaa aatttccatc agtcctagaa attatagagc caataaaact
     1321 tagtccgata tgcggcgcgt tctgtgcgcc gcatattttt ttagttttca aagatcagac
     1381 cttgttgtaa atagatccgt gcccgaaagt tataaaagtt gcgataacca aacgcaattc
     1441 gtttaatcag cttgatcttg ttatacagcc cctcagtgac gccgtttgag tgggacacct
     1501 taaaggcatt tgaaataccc gattggtagc gatggaagat cgtgaatttc tttttaaacg
     1561 actgaggtaa cgtagatgaa attgttttaa gggtttcttg aaagcctacc gtatctcgtt
     1621 taacgaaata atagcgtaat aattgacaaa aatgatacgc agttcgcaat tcttcgttgt
     1681 agcttaacag ctcattaacg atttctgttt gactaattgg tcgtttaaac agtcgatgat
     1741 aatgccgttt gagtgcctcc catgtcgtgg aatctttgag taagagtttc caatatcgct
     1801 tgagccgacg gtacttcttc tgattttcag agtgtgaggt tcgaaaacga ttcatgatct
     1861 ggacacgtaa ttgattgaag ctgcgattga tgtgttggac aatatgaaag cggtcagtta
     1921 cgatctccgc atttggaaag accgttttaa gcaactgaca atagctggca ttcatgtcca
     1981 tcactaggta cttgaccttt aaacgggcct ttctaggaaa gcgcaggaag tattgagtta
     2041 gtttgtaaag tctgcgatct tcaagggtcg taaatagctg attggttgtc ccatcaacac
     2101 aaataaaact catggaacca gaagtggcaa cattgtgtac ttttctttgc aatcttgtgt
     2161 acaaaactat tgcaaaacac agcgcataag aatccttaca aaacagtaga tgaagcaacc
     2221 caattagatc ttgttatagt tgttaatcag ctcttaacgg gttacgattc aaaatgggtc
     2281 aacaccctat acttagataa gtcacttgaa tatcagaaca taattcaggc tttttcacgc
     2341 acaaatcgtc ttaatggtcc tgaaaaaccg ttcggaacca ttcgttatta tcgtaaagca
     2401 catacgatgg aacagaacat tgaagaggct attgagttat actcaggcaa acaagcaaaa
     2461 gggttatttg ttgaaaaatt atataataac ttagaaaagt tgaacgatat attcgaagaa
     2521 attaagtccg tatttaatat ttctggcata gaaaacttca gtcatttacc ggaggttcca
     2581 ggggaaatta agcaatttgt aaatctttat aatcaactaa tccattgtta tgaatcagcg
     2641 atgatacagg gattcacatg ggcaaaatcg acttatgaaa tccaggatta tgaacctata
     2701 acagtagtaa tcacagaata tgaatttaat atcttggcac aacgttacag cgagatagag
     2761 agagaaccta gagacggaaa tgatagtata gcgtatgaca ttgattacca attacctgaa
     2821 ggtagtcgta gtcgtattaa ttatgagtat ttaaacacaa acttcaaaaa atacatgaat
     2881 ataaaagaag aacgtgattc gaatttaatt caatcattgt taaacgactt acatcgcaca
     2941 tttgcgcaat taccagtgga gttacagcca tatgctgaga tgattctaaa tgatgtaaat
     3001 aatggggaat tagttatcaa agaaggatgg gaatttactg attacctcaa tgaatatcag
     3061 cttagtgaag aggacgattt tatccaagat ttagtggatt caaccaatat taatgaggca
     3121 ttattacgtg agatgttgga tttacgctta accgaggtaa acattaatga atatagtcga
     3181 tttgataaat taaaatcaag tgttaacgta ggtcatttca gtggatatat cgagaaaaat
     3241 ttaggtaaaa tggtaatacc aatcaaggta aatatgctaa ttgacagatt attgcgtgca
     3301 tttttattag aagatgtatt cgatgttaca gagtacataa agaattactt taactagagt
     3361 acacctacat cttatagtac ataaaaaaat tcatcttttt tagtttaata attcaccaaa
     3421 aaggtgagta atgccatttt tgaaaattga gcttgtttta tgtctacatc tgtatccgta
     3481 tataatccaa aggattataa actcagaaga gtctttattt tttctgaata taactactgt
     3541 atcaaaaaca atcgattatg agacaaaaca aaatgacctc ttttatttaa gaaaggaggt
     3601 cattttttgt atcataaatt gtattaaaat caatgtatca ctttttaatc aattttaaga
     3661 ccataaattg cgtactaaaa gaaattctgc aattcaaaat aaagcatttt aagccatttt
     3721 caaatttaag tgcacccatc tacccgtcaa atcactttat tttccactcc agtagtttcc
     3781 taattcaact gtgtgcaaaa cgatttcttc taaacaaatc aagacaaact tataaacctt
     3841 catcccagca ccataattat ttaaatcata ggcatttttc gaaattagta tatgtactca
     3901 agtttcactg cagattattt atatttttta gagaaataaa tttttcctat tgacaaacac
     3961 aaagaaagcg gatacaatac aattatgtca catgacgtta tatgacgtta tgtaatatct
     4021 ttacatttaa tcagttactt ttattttgga taaaattaac atttttaaaa ttttaaggag
     4081 gaaatgttga tggatgtagc aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag
     4141 atttctggaa tttatttggt ggcttgtggc ggttcgctgg tagatatgta tgtctcagat
     4201 tattttctta aagccgaagc atcgaagtgt attacaggct tatatacagc gaacgaattt
     4261 aaccatgtaa ccccaaagca attagatgat aaatctgttg tcattctatg ttctcatgga
     4321 ggaaatacag cagaaacggt aaaagcagga gaagtcgctc aagctagagg agctgtgact
     4381 gttggtctga ctcataataa aactgcggat ttattaaagg tttctgatca tagtttcctt
     4441 tacgaatggg gagatgatgc aaaaatagtc aataatccta tggccatcat tcttgattta
     4501 actgtatctt tggtcaatgt taccgaaaca tttgaacatt acggtaaatt cagagaaggc
     4561 atgaaggtta tcgataccgt aattgaaaat ggcattaagc aggtgcagga tcgtattaaa
     4621 gtgtttgctg ataagtacaa agatgaaaca atgttttata tcttaggtag tggaccatca
     4681 tatggacatg cttatggttt ttcaatttgc tctttgatgg aaatgcaatg gcttgattca
     4741 acttctgttc attctggaga attcttccat ggaccttttg aagtaacaga caaagatacg
     4801 aattttattc tcttgatgag cattggacgc actcgtgctt tagatgaacg ggcaaaacga
     4861 ttccttgata aatatgcaga aagagtagaa atcgttgatg caaaggaact aggattggat
     4921 atgatatcag atgaagtttc tgaattcttc aatcccattt tattctacag tattctttca
     4981 gaatatcgtt cagctttagc agatgcacgc aatcatgatt tagatgttcg tcgctatatg
     5041 gggaaagtgg agtactagga ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt
     5101 cgataaatat gaaaatctgg gaattatgta tcctggagga aattcattga attttgcagt
     5161 ttttgcaaaa aaactgggac atgaaagtag ctttatggga gtttttggct cagatgagga
     5221 ggcagctcat gttttatcag caataaaaaa aataggactg gacaacagtc actctcgctt
     5281 tgaaaaaggc gagaatggct gtgccagagt gaagattgat gagggcgacc ggattttcct
     5341 tggcagcaat tcgggaggtg ttactcgtga atacccgatt cagcttactg aagaagacag
     5401 agaatattta aaccagtttg aattgattca tatgggatta tatagccatg tcaatcatct
     5461 attagaagag ttacaaaatg tttctggaaa gatttcttat gatttttctg acgactttac
     5521 ggaagaagag gttcagcggg caattgataa agttgatttt ggcttctttt ctatagaaga
     5581 cttttccgat gaagaagtaa agcagttttt gatgaagcat ttttgtgagc gaaatgatgt
     5641 attgattgca acccgaggag aaaaaacggc tattggttat gatggagcaa agttttatga
     5701 agtgaacccc gtgctagaag aaccagttga tacaatggca gcgggagatt ctttcttaac
     5761 agctttccta ctttattttc tcgaagggga cgatatagtc accgcgatga aaaaggggaa
     5821 tgaatttgct gggaagtctt gtctggttga cggttcgttc ggctttggta ttcattacga
     5881 ttgagcttgt tttaaattcc tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg
     5941 aggttaaaaa aatgctggat acaaatgcac aattaccatt gtacttacaa ttaaaagaaa
     6001 tattaaaaaa taaaatcaat acaggggagt atcccgagaa tacgaaaatt cccgctgaat
     6061 cagagctgat agaaagttat gaggtcagcc gaatcacagt gagaaaagca gtagaagaac
     6121 tggtagaaga agggtaccta cttaaaaaac aagggaaagg aacttttgtc aaacggcata
     6181 aagtttttcg taaaattgaa tatgttatgg gtttcagtga gagttgtaaa tctaatggtt
     6241 ttacaccgac tagcattgtt ttggaacgca agatcattcc agcaactgaa gggatggcaa
     6301 aaaaactcga tatcgctcaa ggtgatgatg ttttatatat ccagagaaag cgaatggcgg
     6361 ataacatccc cattatgtta gaaaacaatt atttttctta tgaaagatat agtttcttaa
     6421 tggaggaaga gctgactgat tctttgtatg aagtacttaa agtccaaggt accttagcca
     6481 tcaatcctgg ggaaactgca cttgaaattg tcacagctga tgaccaaatt gccaaaataa
     6541 tggaagtagc tgttgggacg ccctttttct ttatggatac tttaatcaac gatcaaaaca
     6601 acataccaat tcatgtaggt aatcaatatt acctgggaga agtatatcgt ttttacttat
     6661 gatttcctgt tttgatttca aggagtagat tttatttatg aagcaaatat tgcggtcaaa
     6721 aatgattttt gatggagagc agccaccttt caaaggttat attgtaatca atgaagggat
     6781 cattgaaaag gtggaaaaca actggaatta cagtgatcta ttaaatgaaa acacggaatt
     6841 aattgcttat gatgatcaat tcgttatgcc agcaattcat gataatcatg tatttttcag
     6901 tggttatttg gctatgaatg ctggcataga tttaagcgat acatcttcag ctaacgaggc
     6961 tgtagagcgt attaaagctg taatgaacca taaaaaagca gatgaaccaa tttatgctca
     7021 tggctgggat gctgccctgt ggaaaactga cccatcagaa gagctattga atgaaataaa
     7081 ctactttggt ccaatcacag cgatcgacaa aaatcgcagc cattgttgga tgaatgctat
     7141 tgctaagcag acttatggtt ttacagaaaa agaaaccagt gctgaaagtc gagtattatt
     7201 aattaaggaa atgctagcaa acaaaaagct tttagcagag gtttacgctg aatttgaagc
     7261 attactgctt tcaaaaggtg ttatatcaat gaaagaaatc gtttttgatg acgcagaatt
     7321 tctttctaat atacctcaga ggaaaatact tagtaaccta tatgtgcagg cagtccaaga
     7381 acctctacag catgaacttc tatatgagta taaggaaaaa gttttccctg aaaaggtccg
     7441 ctttggtgga gtgaagatca tggtagatgg tgttgttgcg gatagaagtg gagatattta
     7501 cggtgtttat cagtcaggag tagcttcacc cgagattgac tatgaggcaa ttaaagcaga
     7561 agttgctgca ttcaatgaag tagagattcc ttgctgtttg acaacggaag gagataaagc
     7621 gggttataaa gctgcgtcaa ttcttgtcga atttggaaag cggttacctg aaggcgtttt
     7681 taattcgatt agtgatttgg aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg
     7741 acaagtagta gcagaggtat atccacaaat tctcggcttg aatcaaacgt tagaagaggc
     7801 ttacatgtca actgttataa aaaa
//
LOCUS       DAFBQW010000103         7707 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFBQW010000103 DAFBQW010000000
VERSION     DAFBQW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.108"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            546..1109
                     /locus_tag="LK889_002745"
     CDS             546..1109
                     /locus_tag="LK889_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5096928.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1388..1675)
                     /locus_tag="LK889_002746"
     CDS             complement(1388..1675)
                     /locus_tag="LK889_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5096929.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1665..1994)
                     /locus_tag="LK889_002747"
     CDS             complement(1665..1994)
                     /locus_tag="LK889_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5096930.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2299..2628
                     /locus_tag="LK889_002748"
     CDS             2299..2628
                     /locus_tag="LK889_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5096931.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2915..3400)
                     /gene="vanZ"
                     /locus_tag="LK889_002749"
     CDS             complement(2915..3400)
                     /gene="vanZ"
                     /locus_tag="LK889_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK5096932.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3553..4470)
                     /gene="vanY"
                     /locus_tag="LK889_002750"
     CDS             complement(3553..4470)
                     /gene="vanY"
                     /locus_tag="LK889_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBK5096933.1"
                     /translation="MKKLFFLLLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKE
                     HLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYG
                     LLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPA
                     GYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWH
                     IRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPT
                     NLRYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4898..5506)
                     /locus_tag="LK889_002751"
     CDS             complement(4898..5506)
                     /locus_tag="LK889_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK5096934.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5512..6543)
                     /gene="vanA"
                     /locus_tag="LK889_002752"
     CDS             complement(5512..6543)
                     /gene="vanA"
                     /locus_tag="LK889_002752"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK5096935.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6536..7504)
                     /locus_tag="LK889_002753"
     CDS             complement(6536..7504)
                     /locus_tag="LK889_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK5096936.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2357 a         1533 c         1281 g         2536 t
ORIGIN      
        1 agttaaaact tttttagcag attttaaagt ttcgtcaaaa ctaatatatt ctggtagctg
       61 agcattcaaa tagctttcag acttcagaag aaaattacga gcatttttat agtccatctc
      121 taataaagtt attctttttt tatccaatac attcagctca attccttata atattctatt
      181 ccttagtatc tcaacattat ttactttata aaacgaataa attttgtagt tctaattcaa
      241 atattgcatg ttatatctaa tatgggagca ttttataatt ttcattgtga taaactcgtt
      301 attaacttta ggatacacta gttaggatta aattaggtat ctagaaattt acttaagatt
      361 tttttgttta tttcgtatac acactcgtat caaaaacaat cgattatgag acaaaatgaa
      421 atgacctctt ttattaaata aaagaggtca tattttgtat cataacttgt tgcattaact
      481 atgtatctat aactcccttt tatgatacaa tcaagccaga attaaagtac gaaaggaaga
      541 aaagcatgag tggatacatt tatggttatg cccgagtgag tacacgttca caagaactaa
      601 atcgtcaatt agacttactc aatgaacaaa attgtaatga aatcctaact gagaaaatga
      661 cgggaaccaa agcggttcgt ccccaactaa atcgtttaaa agataaaatt cgtccgggag
      721 atacggtaat tgtagaaagt ttttctcgat tgggtcgtag tacaaaggat ttgattgacc
      781 ttgttaccta ttttgaagaa caccatgtta agctggtgag cttgaaagaa aacttcgata
      841 ccagcacgcc tcacggacgc ttaatgatga ctgtcttcca agcatttagt cagttcgaac
      901 gtgatttaat tgttgaacgt accaaagaag gtttgaaaag tgcacgtgcg agaggtcgta
      961 aaggtggccg tccacgagtg aaccagaaag atgttgatcg agcagtaaaa ttgtataaaa
     1021 gtcaggttta tagcgtaaaa gaaattacgg aaatgactgg tattagcaag gcaacactct
     1081 accgctactt aaaagacaat agggaataat ctattttaat ctttaggaat ctataatatt
     1141 tatgggagaa ttagagtgat ttttttatta ttactaaaaa acaatataaa ataacgcatt
     1201 ttaagccctc tttgtgcata aggatgaagc ttttaatttg aaagaggctt aaaatgcgtt
     1261 ttaatttttt ctatttttca taaatatata ggttagtggt ttaaaattat agggtattac
     1321 aactctcagc tgttaaaaat tatcttttaa agtgtcgtct atcctctcaa gttgaaacat
     1381 tgggttctta cctaatcttt tttaattgtt cataaaaatt ttcatgcgtt ccacatagta
     1441 agataggtat cactgcatta tctttaattt catatgcaac gcgatatgtc gtacctgcat
     1501 acttgaatcc aatagcccaa attcctgata aatcacccgt tttttgttct cccgaatgag
     1561 gacgaactgc tatctcatca taaattatcg ttaaaaattg ttcttttaat gttttctctt
     1621 ttaatttttt tagatacttt attacgtgtt tagaaggtag aatctcataa gccaatttct
     1681 gctgccaact cctcttttgt catcggttta gaatttgcaa cagtatcttc agcaatagat
     1741 ttaaatgcag aagttaacgc attttttcta tttctaaatt cttttaagat ctcttgtttg
     1801 ctataacctt ctgaaagaat atcagccaaa atatcttcac taaaatcaaa aaattctttt
     1861 ttaggctcta caaactcata aaggatacca ttctctacaa gcttagcttc tacttcaaca
     1921 ccattgggca catcgaattc tttagggacc gttaacataa tagaatttcc ttgttttctt
     1981 gtttttgtaa tcatattatt tcctccatta ttttacattt cgtaattact tagtaattac
     2041 attgtacgct atttttagta gaaatacaaa ataataaaac tcctttagaa caattttcaa
     2101 aggagtttta tttgattagg aaattatgta gaataataat atgtttttaa cttggaatca
     2161 aaaacgcact tgcaagtaag tttacttcaa tacatgaaat tgcttataat agtgtattgg
     2221 aattaaggta gattgacaaa agagtgatca aataggtata cttcattcaa ataatcgttt
     2281 gaatgtgagg tgagatgaat gaaaaaagtt tgccaagtga ctgtcgtaca taaagaaaaa
     2341 gtaaatggaa taaaaaataa attggatcaa aaagattttt caaacttact tgtgttaggg
     2401 aaatgtttca gcgatttttc tagaattaaa attttctatg ctttagaaac ctataaggaa
     2461 atgtgcgtct gtgatttagc agaaatactt actgctagtg tcgctacaac gtcacaccat
     2521 ttacgttttt taaaaaaaca tggaatggca aaatcacgtc aagatggaaa agtagtttat
     2581 tactctttag cgaatgaaga catcctttcc gctattcgtg ttttttaaag ttatcagaaa
     2641 atctatctat gaaatcttag tttgggggta ccgtcaggaa aatgcggatt tacaacgtta
     2701 aggaaattta ttaaagagac caattcctca gcactaaaat tgataattgg gctttctttg
     2761 ttactatagt gttaggtaag ctttttactt tatcaccggt tttcccctca cttcacacct
     2821 acgggcgagt ttccccacat cccgttttcc atctaatcca attcattcaa tatctttatg
     2881 tgtatgaaag atcagattgc ttgatttaga catcttacac gtaatttatt cttaaatggg
     2941 tacggtaaac gagcaataat acgagcaaaa gtatacctac aaaaataata actctgtcta
     3001 attttttttg attcatatgc ttattgctta aaccatataa tttcagtcca agaaagcctc
     3061 caacagtatt tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa
     3121 ttatttcaaa agtaagactt aaaaccagta caaaagcaaa cttaggtaaa aatccgattt
     3181 ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca
     3241 tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat
     3301 taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta
     3361 aatataaagc tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt
     3421 ttgtttaagt tgtatcatta gtatagcaat gtcctagtta acgtgggttt taaattaaaa
     3481 agctaacgtt accccaaaag tgctagtaca ataatgctgt tcattaattt cgttggtttc
     3541 attccgccat ccttacctcc ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat
     3601 tacaccgtct atattgtttc ctgatatctc ataacgaaga ttagtcggca catgaatggt
     3661 ggtattttta gtaacaggat aataaaagat ctcatatttt tccccattta cactaacaga
     3721 aatggttttt tcttctttta ggtaatccat atattcctcg agaacgaaat tcttttcttt
     3781 cataatcgca ctatgtggta aaccaacata gcgaatatgc catagttcat attgaattcc
     3841 tgttaactct gttttgtcct ctggataacg taaaatgaac ccgtatttcc aagcattttc
     3901 ttctatccac tttccttcag gggctcgttc cattttcgtc aagcttgatc ctacatctag
     3961 tgataaacct gaattatgct cactataacc tgctggtaag gcatactcag cccccatttc
     4021 ttggtaaagc acactttgct catcaaagtc tcgatagcca ctattaataa taaaatgact
     4081 aacgccaccc tttacagcat cattgaccat ctctgaaaat ttttgtgcta tttcttttga
     4141 catataaata ttactatcaa gcaacccgta tccatttatt aattcgtcat gtttagataa
     4201 attcacgata tctgacttca cactttcttg gcgaacagga tatttactat tgattaatag
     4261 cagatttcct tgataaacct gttcttctgt aattgttttc tcttgggtat tttcagaagt
     4321 cccactattt tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt cgactttttc
     4381 ctgagaaaac agtgcttcat taacgtagtc ataacctaag tatattaaga ataacaataa
     4441 caataacaat aaaaaaaaca acttcttcat tttcagtctc ctccttaact atatagttgg
     4501 aagtatgaaa tatttctaga atcttcccct taaacggcac aatctcgttt gagattgtgc
     4561 cgttttgccc cgtaaattac agtcaattat ttcgccctac tggatttact attatcacca
     4621 atgtagatga attttcataa gtgataccca tgctgaattt aacgaggctg aactgcctgt
     4681 tgacaatggg cggtgtgaaa taccacccct ctatgcgatc agtcttatta tcaggctatc
     4741 ttgccgcagc gcacgcagat aatgaccgca atatcgctct gccttacatc ttcaatacta
     4801 tctactatat ataaattccc taagaaccag ttacatacgt cgggtttcct gccgaaagag
     4861 ttagcttata tagtttgtcc gtgcaacggt taaaagttta tttaacgggg aaatcaaaat
     4921 agctattggg gtatggttcg tctcttaata catagtgcca ccattcgagg ctatatgctt
     4981 caaacccact gttttccatg atggagcgca aacgtctgcg attttgcgct tcattgcatg
     5041 atattccatt tgccgcatga tgagagcgtt catccataaa atcaaatcgg ctccccattg
     5101 gtacaagctc acccgtgtct aatcgataaa gcgtaagatc aatggcactg ccgcggctat
     5161 ggcttgattt tgaagccacg tatccttttg aaatcatctc agttcggtca atattgggat
     5221 aataactttc ctttgtcagg ttattttccg gctgtgcagc ccattgcata aaacagttta
     5281 cagcacgatt aggacggtaa ccgtcccata gaagcaatcc gtacccttgg gtagcagcca
     5341 gttcttttgc cttcaaaagc gattcagcca actcgtatgt ccctacaatg cgatttactt
     5401 cataaccgtc aaccggtttt ccggtgaaat tatcccaagt ggcatattta gcgtcccaac
     5461 gaacaccgtg tactatttca tctaaaaaag taaatcctat ttccatgctt atcacccctt
     5521 taacgctaat acgatcaagc ggtcaatcag ttcgggaagt gcaatacctg cagcggccat
     5581 catacgggga taacgactgt atgacgtgaa accgggcaga gtattgactt cgttcagtac
     5641 aatgcggccg ttatcttgta aaaacatatc cacacgggct agacctctac agccgagcgc
     5701 tttatatatt ttttttgccg tttcctgtat ccgtcctcgc tcctctgctg aaaggtctgc
     5761 gggaacggtt ataactgcgt tttcagagcc tttttccggc tcgacttcct gatgaatacg
     5821 aaagattccg tactgcagcc tgatttggtc cacctcgcca acagctaacg cggcactgtt
     5881 tcccaatacc gcacaaccga cctcacagcc cgaaacagcc tgctcaatta agattttgct
     5941 gtcatattgt cttgccgatt caattgcgta gtccaattcg tccgcgctat tgactttttt
     6001 cacaccgaag gatgagcctg aacgcgccgg cttaacaaaa acaggatagg taaacgtagc
     6061 tgccaccggc ctatcatctt tattaataac ccaaaaggcg ggagtagcta tcccagcatt
     6121 tttcgcaacg atgtatgtca acgatttgtc catacaaatt gctgagcttt gaatatcgca
     6181 gcctacaaaa gggataccgg acaattcaaa cagaccttgt atggatccat cttcacctga
     6241 cttgccatgc aaagctgaaa atgctacatc aacatggttg atttcatatt catggttctt
     6301 tttaacaagt aatccgtgca tttttttatc cggcgagagt acagctgaat agcaattgtc
     6361 gttttcccat tccgcgcaag gtttttcgca cattttccat acaccagatt tcgtaattcc
     6421 aatgtataac ggctcgtatt tttctttatt aatgttagcg gctatctcta ttgcagattt
     6481 taccgatacg tcatgctcct ctgagcaacc cccaaacagt attgcaactt ttattctatt
     6541 catgctcctg tctcctttca aaatccaaac agtttttaat ggttttttca acggtatcac
     6601 gcaacgcttg ctcggtataa taggccgtat gcggtgtgat tatcacgtta ggcattcttt
     6661 gaagtttaag taaaaattga ttatcaattg gtttttgggt gcaatcagag tagaaaaact
     6721 cttcctctcc ttccaataca tccaatgcgg caccgcccag tttcccgttt tctaatgctt
     6781 taaccaactc ataggtatct acaagtggac cgcgcccagt attgataaga aatgctcctt
     6841 gcttcattct ctgtatttgt tcgtggctga taatatagtg cgtatccgta ttgagcggca
     6901 catgaagcgt aacgatatcg ctattttgca gcaactcatc aaacggtaca tagtttacct
     6961 ctatacttcg gctgcgacta taagccaaca ctttacatcc aaatcctcgc agccgctcaa
     7021 taaccgcttt gcctatctgg cccgttccca ccacaccaac tgtcatgtcg ctgagtacct
     7081 tgccacggtc gctgtccaac ctgaaatcat gtttttccac agagcgcaca atcgatttta
     7141 cgttgcgtac tgccataaga attagcatca tagtataatc ggcaacgcta tccggcgagt
     7201 acgccacatt gtcgacagtg atgcccattc tcttagcagc agttgtatct atatgattgc
     7261 agccgatgct tcgggtagaa atatatttca caccggctct cttcagcgca agaagaatag
     7321 aggcggaaat ctctgattta tgtcccacac tgatacattg attgaaaggc gcggatttgg
     7381 cgttggattc cgacacgttg gcgttaatta tcgttgccat aacgccaaag cgaggcgaaa
     7441 gagcatggaa tgcatctgcc tcatcctgct cacatccata aacagtaatg ccgatgttat
     7501 tcataattaa gaccaaccct ttctgtgaaa ggcaccaaat aaaacgggga tgccaatggt
     7561 aatcttgtca aatctttctc tgaagaacga aaacggctcg ttcttacggt attaagtcta
     7621 ccgtgtaaga acgagccgtt tcttaatatt aaattgttaa tttcctaaga aaaagtaaag
     7681 ataaccttga gattttccta aaaaatt
//
LOCUS       DAFBQW010000104         7598 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFBQW010000104 DAFBQW010000000
VERSION     DAFBQW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.109"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(941..1354)
                     /locus_tag="LK889_002754"
     CDS             complement(941..1354)
                     /locus_tag="LK889_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBK5096937.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="LK889_002755"
     CDS             complement(1810..2295)
                     /locus_tag="LK889_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096938.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="LK889_002756"
     CDS             complement(2434..2649)
                     /locus_tag="LK889_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBK5096939.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="LK889_002757"
     CDS             2873..3673
                     /locus_tag="LK889_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5096940.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="LK889_002758"
     CDS             3692..5560
                     /locus_tag="LK889_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK5096941.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="LK889_002759"
     CDS             5553..6044
                     /locus_tag="LK889_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBK5096942.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="LK889_002760"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="LK889_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK5096943.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="LK889_002761"
     CDS             6604..>7598
                     /locus_tag="LK889_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK5096944.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DAFBQW010000105         7000 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFBQW010000105 DAFBQW010000000
VERSION     DAFBQW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.110"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..174
                     /locus_tag="LK889_002762"
     CDS             <1..174
                     /locus_tag="LK889_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096945.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            167..307
                     /locus_tag="LK889_002763"
     CDS             167..307
                     /locus_tag="LK889_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK5096946.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            377..796
                     /locus_tag="LK889_002764"
     CDS             377..796
                     /locus_tag="LK889_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK5096947.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            793..1803
                     /locus_tag="LK889_002765"
     CDS             793..1803
                     /locus_tag="LK889_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5096948.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            1918..3126
                     /locus_tag="LK889_002766"
     CDS             1918..3126
                     /locus_tag="LK889_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096949.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            3110..3280
                     /locus_tag="LK889_002767"
     CDS             3110..3280
                     /locus_tag="LK889_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096950.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
     gene            3535..3608
                     /locus_tag="LK889_002768"
     tRNA            3535..3608
                     /locus_tag="LK889_002768"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3569..3571,aa:Ile,seq:cat)
     gene            complement(4121..4423)
                     /locus_tag="LK889_002769"
     CDS             complement(4121..4423)
                     /locus_tag="LK889_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096951.1"
                     /translation="MLIEGKTQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            4402..4809
                     /locus_tag="LK889_002770"
     CDS             4402..4809
                     /locus_tag="LK889_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096952.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENYWIGEEKLNEIY"
     gene            4796..5248
                     /locus_tag="LK889_002771"
     CDS             4796..5248
                     /locus_tag="LK889_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096953.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            5241..5639
                     /locus_tag="LK889_002772"
     CDS             5241..5639
                     /locus_tag="LK889_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096954.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            5951..6024
                     /locus_tag="LK889_002773"
     tRNA            5951..6024
                     /locus_tag="LK889_002773"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5985..5987,aa:Ile,seq:cat)
     gene            6404..6565
                     /locus_tag="LK889_002774"
                     /pseudo
     CDS             6404..6565
                     /locus_tag="LK889_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            6715..6915
                     /locus_tag="LK889_002775"
     CDS             6715..6915
                     /locus_tag="LK889_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5096955.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2513 a         1011 c         1296 g         2180 t
ORIGIN      
        1 agtggcacag cggttgacga tattgaagat gtcgatcagt tgaagcaatt aaatgctcta
       61 ctgacaaaac aattggcgat atcggtacag gaacaagaaa aaatgcaaca aatgttagct
      121 caattgacga tggaagtcgc aagtattaag aatggaggga aaagtaatga atagttttcc
      181 gggctttgaa aatataaaac aattttatga ttggggatgt tatacggacc aagatttact
      241 tgactatgta aacatgaact gtttaacaaa agaccaatat aagcagatta ctggaaatga
      301 aatttaatta agcacaaagt taaataaaaa gcgtactcaa acgagtgcgc ttctttgcat
      361 aaaggagaaa tagatattgg aaaaatattt aaatacactc tcagtaatga caggggtgat
      421 aggcgggaca attgttggtt tattgggagg gatggacaat atactacatg ttttaatttt
      481 tcttgtgggt gtggacttct taactggact tgctaaggca tggaaattga aagaaataag
      541 tagtgagata ggtttcgaag gattgttgaa gaaagtctta atcttcgttg tgattgccgt
      601 tgcggttgac gtacagaaaa tcgttgggaa ttctatacct ctaagagaaa tagttattat
      661 gttctatgtt gcaaatgaag ggattagttt tttagaaaac atttcggtat tccttccttt
      721 gccagataag ttaaaagagg tattccaaca aatacgaaat gatactgaaa ataaagaccg
      781 aggaggaacc aaatgaaaaa gaaaatcttt gtaggagcta ttatagctct ttttttattg
      841 ccaataaacg cctttgctta ctcgatcaac aatgaattca atttgggcgc aaacgaaggt
      901 agttctcaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
      961 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
     1021 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
     1081 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
     1141 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
     1201 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
     1261 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
     1321 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
     1381 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
     1441 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
     1501 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
     1561 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
     1621 gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc
     1681 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
     1741 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
     1801 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
     1861 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
     1921 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
     1981 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
     2041 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
     2101 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
     2161 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
     2221 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
     2281 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
     2341 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
     2401 attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt
     2461 atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat
     2521 gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat
     2581 ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt tgttatgaat
     2641 attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga
     2701 tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat
     2761 ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat
     2821 acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg
     2881 ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta
     2941 ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca
     3001 attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat
     3061 tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat
     3121 aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa
     3181 taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa
     3241 taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt
     3301 ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga
     3361 aaagttaaag taaataatag cataaaataa tatattttac aaagaataag tacaatctag
     3421 ttttttgcta ttaaatgtgt aataataaat atgccatcac aacatgaaga atgaaatcca
     3481 ttattatcta gtctagtcca ttctttttgt ttgcagtatt tgtgatggct ttccgtaccc
     3541 ttagctcagt tggtcagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
     3601 agggtacatt aacgtagcca tttgaatcgt tctgtgttag aattttttga agagtattat
     3661 acaagctaaa gcttttcttc attgccactc aaatgagtgg cttttttatg tatcctttta
     3721 tggattaatg aaaggatgtt tcacatagtt atatttctgt atatttgaaa agttttactt
     3781 tgatttttaa atagaaagac atttgggtta aattgtgaga taataataaa gaagagttta
     3841 aagcgcacct caaaccactt ccccataagt gtgttacgct ttaaactctt ttatatttga
     3901 agctattaaa aggcatacca tatttttgaa aaaaagtgag aaaaaaggct tacaattgga
     3961 gtggtagtta attagtgact tatttttgat tttatagcac tgatactata aaatatagat
     4021 atcatcatat tacacaatct taatactaac ttaaaaaata tctcctttca caagtatggt
     4081 gataaaattc gttccgggct acctttttag gtagcctact ttaatcttta tacctttctg
     4141 gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt gcgtccggca
     4201 cgtcagtcac gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag
     4261 ggattttaaa gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg
     4321 ctagaaagaa aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag
     4381 gatcaaattt aaaccataat tgtgtctttc cttctattaa aatatcttga aatttactct
     4441 gttaaaaaaa ataaattaat tggtgaaaat gattctccaa taaaaacacc tattgagcta
     4501 agcttaagaa atcggatggg aatactccaa agatcggaaa tagtaaaaga acttttttat
     4561 cataaattta gttatgaaat aagactagat gatacttatg agcattatag aattgttttt
     4621 ttcgtataca attctataga agatgctact gcgactactg cattaacatt cggttttacg
     4681 aaaaatggaa caatcaattc ggataaaacc cgtcaagctg ctacagaaag tgatgatata
     4741 tgtaagaaag tatgcaatgg ggaagagaat tactggatag gggaggaaaa gttgaatgaa
     4801 atctattaat gatttattaa aaaaacatcc tgatttatat aaaaaattaa attctgaaca
     4861 aagaaacctg tccaaacaat tacttaaaat aagattaaaa aaacaaaaaa acgtgacaga
     4921 attctcaaaa atgttaggaa taagtgaaaa aaaatatctt gaatttgagt tcggagatat
     4981 gcatattcct gttgcagagt acaataaact tttgaaacaa gttgaaaatt tgtcctataa
     5041 agattcaatg aatttcacta ttaaggttgc tggcggccct attaaatatg accgtattga
     5101 aaataaacct tatattcgga ataaaagtta ctcagtattt aaaagtagaa agagaaaaga
     5161 agtatcgtca aatatggtct atgctagtgt taattaccaa gatacgagcc gctatattgt
     5221 agaaaaagag gtatatgtac atggataaag gaaatagtgt tacttttgca cttgaaaata
     5281 ttagtttgaa taaaattcat tttgaattaa ctgataattt tagttatgga gatttagagt
     5341 ttaatattga acttggatat aaagaagacg aagcagagta tctagaaaaa aaacttgttt
     5401 ctttacgagc tcacattgta ggggggatgg aaggagcacc ggtagatatt ttactctttt
     5461 cagttattcg tgtttctaca aatgaacagc catataaaaa attagtagaa gaatctatgg
     5521 gagagctagc taaacctatc ctgaataaag cttctaattt gatttctaat ttatatagcg
     5581 aagtaggagt tatgcctaca gttattgatt tatatcagct atatagtcaa caaaaataaa
     5641 atacaagtaa tagagttatc tatttaaatt tgcaagacaa agacctcaac cagtcatcct
     5701 tggttgaggc ttttttgctc tgtaattaac tcgtatagat cctgcaagat agtttgtttc
     5761 tgaataaatg attgatatga agtatttaga tgactgtatt gacaactata aaaatcattc
     5821 gataaaatag tgatgttatc gcatatcttc actatcaccc ataatagtca cactccaagc
     5881 tatgcgataa caggtttgtt gccacacatt ctactggttg attgtttatg gctttatgtg
     5941 gcaacaacca gtacccttag cttagttggt cagagcagac ggctcataac cgtccggtcg
     6001 taggttcgag tcctacaggg tacataattt aatctatttg ataaaaaata aaattcaata
     6061 atggtataat aaaatatcgg ctcgtcgctt ccacagagat tttcgtggga ggaggtgtat
     6121 gatttgtttg aattcacttt ggagcacgtg ctcttgccat tgttaattgg tttaactttg
     6181 gcattattta gtcattggct agataaacat tagagccgac aatggcgctt actgtttcgc
     6241 agcggtaagc gtcttttttt gaaaaagaaa aaaacacaac gtattcgcag gtacgttgtg
     6301 tttatggtgt atatttgttt aaattcactt tcaattatta ttatacttgt taaataaatt
     6361 gtgtcaatat aaaagatatt tttatacgtg tactaaccta tattatcctt ctggtaatgt
     6421 tattccatcg cagcaggata tgaattttac caaaaggata caaaagtgtg gagaaatgat
     6481 ggggatcact gtactggacc acttgatcat cggacgcaaa cgatatttta gtttgagaga
     6541 agaaggaatg atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac
     6601 aattatattg taattttgtt tgggaataga agttgaaaca tctgttgcac ctctatcgta
     6661 cacgagcaat gttacaattt aaattagtgc ttttggcacg aggaggatat tattatggaa
     6721 aacggaacag taaaatggtt caacgctgaa aaaggatttg gatttatctc acgtgaagat
     6781 ggaagcgacg tattcgttca cttctcagct atccaaggtg acggattcaa aacattagaa
     6841 gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc gcggcccaca agctgcaaac
     6901 gttgtaaaag cttaatttta tctgtaaatc aaattgatgg tagacatcaa tttttacgag
     6961 agaacaaagc gagtaacaaa aatacctgcc acaagagaca
//
LOCUS       DAFBQW010000106         6962 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFBQW010000106 DAFBQW010000000
VERSION     DAFBQW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.111"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..193
                     /locus_tag="LK889_002776"
     CDS             <1..193
                     /locus_tag="LK889_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096956.1"
                     /translation="GRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPL
                     VPKGYDFLDSISKLEKMND"
     gene            271..945
                     /locus_tag="LK889_002777"
     CDS             271..945
                     /locus_tag="LK889_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK5096957.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            938..1102
                     /locus_tag="LK889_002778"
     CDS             938..1102
                     /locus_tag="LK889_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096958.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            1178..2320
                     /gene="dcm"
                     /locus_tag="LK889_002779"
                     /pseudo
     CDS             1178..2320
                     /gene="dcm"
                     /locus_tag="LK889_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            2310..2600
                     /locus_tag="LK889_002780"
     CDS             2310..2600
                     /locus_tag="LK889_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096959.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2859..4043
                     /locus_tag="LK889_002781"
     CDS             2859..4043
                     /locus_tag="LK889_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5096960.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4040..4177
                     /locus_tag="LK889_002782"
     CDS             4040..4177
                     /locus_tag="LK889_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBK5096961.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            4923..6833
                     /gene="ltrA"
                     /locus_tag="LK889_002783"
     CDS             4923..6833
                     /gene="ltrA"
                     /locus_tag="LK889_002783"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5096962.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         2437 a         1118 c         1539 g         1868 t
ORIGIN      
        1 gggtcgtatg agtgaactcg gatattccat gatgtttggg gaggtcgaaa aaaacttctt
       61 tatgaagcac atcaaaggtc gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt
      121 ttatacgcca cttgtaccaa aagggtatga tttcttagat tcaattagta aattagaaaa
      181 gatgaatgat tgagtgatct tcaatactga ctttatactt caagtaacgt ataatacttt
      241 tatatagtag gaagaaaggg gataattaat atggaaagta atgattctta tttagagtat
      301 gcaaaagcaa ataaggataa gtttatttct tatattatca aagggaaatc ccccgaagtt
      361 ggacgagacg cagtttttat ggcagggagt cctggggctg gaaaatcaga ggttgccatt
      421 ggattagctg agaattatga taaccatgtg attattgatg cggattactt tcgcacccag
      481 tttcctgatt ataatggaaa aaactctagt ctctttcaaa aagcatcttc ttggttggta
      541 gagcaggcat ttagatatgt cttagatcat ggctattctt ttattctgga tgcaactttt
      601 gcaatattaa gtgctgaaaa aaatatctcg cgggcgttga aaaaacattt tcgagtgacg
      661 attttctatg tttatcaaga ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt
      721 gcagagggac gtcatgtgcc gaaaaaggca tttattaacg cttttttcaa atcaagagag
      781 aacattgcaa aagtaaagag taaacatcca gatgtattgc ttcatatcat aataaaagat
      841 tatacgaata atatttccga agttcattat gctacggata atctagaatt atttttacca
      901 ctagggtata gtaggaaaga tttggaggtt caattacatg actgagaaag aacaagtaac
      961 gaaaattgta aagaaataca ataagagcat tgcggattta tcggaaaatg ctactgcaaa
     1021 agagttcaaa acggtgatta aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt
     1081 agttggatta aataaaaaat agtaggacaa attaataatg tatgagccaa gagcaaaatg
     1141 ttcttggctt ttttctttgc aatgaatgag gtaatgtatg agatttttag atttattcgc
     1201 cggtattggt ggctttcgtt taggtatgga acaggcgagc catcattgta tcggtttttg
     1261 tgaaattgat aaatttgcta gacgtagtta taaagcaatt catgatacaa gtaaggaggt
     1321 ggaaatgcat gacatcacaa gtgtatcaga tgagtttatt caatccctcg gaccagtgga
     1381 catcctttgc ggcggatttc cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt
     1441 ttccgatact cgaggtactt tattctttga aatcgctcgg ttcaccactc ttctccgacc
     1501 taagctttta ttccttgaga acgtccgagg attgctcaat cacgaaggag gggctacgtt
     1561 cgagacgatc ctccgaacgc tggatggatt ggggtatgac gtggagtggt aggtgcttaa
     1621 ctcaaaggcc tacattcccc aaagccggga gcggatattc cttatcggac attctcgaga
     1681 cgcatgtacc gaacaagtat ttcctatcat tggaccgtct tcaacatctg atcaaaacat
     1741 cagaaatttg gtaaatatca atccttccaa tcgaggaatg ggtggacaag tctatggttc
     1801 agatggcgta gcgccaacct taacaggtga tgaaggaatc aaaatcgcac tgccagcaag
     1861 ggatggaatc tctgtagcag gactattacc gggaaagttt gaacaaggta atcgagttta
     1921 tgaagttact ggaaccgccc ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat
     1981 tcgaaaaaat gaaacagaat atcaagaggt gaagccagaa gacagtgtga atctggcttt
     2041 tcctaattcc acaagtcgcc gaggtcgtct gggaaaacag agtgttcata ccttactaac
     2101 aggagaccaa caagcgatcg tcacggatca gtatcaaatt cggaagctaa cacctagaga
     2161 gtgttggcga cttcagggct ttcccgattg ggcttttgat cgagattctc aagtcaattc
     2221 agatagtcag ttgtataagc aagccggaaa ctctgtaaca gttccggtca tttttgatat
     2281 tgccagaaga ttaaaagagt tggatgtaca tggtttatga tgagttgatt ggagaaattt
     2341 attgggtgat tggaaagatt cagtcagacc cagaattaga agaacaattg cgacggttaa
     2401 aatttgatat tcgcaagaat ggtgtcaaag tcgctggcga tccatattta atgaatgagg
     2461 agacggatgc tcgaattgaa ataaatcaag taattgcaga atttgagcgg attgcggacc
     2521 aagcaaaaga accagatatt cgtcaatact tgttagaaat gaaagcagag ttggaagtaa
     2581 atggtattac agatgagtaa cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga
     2641 ggattcgcga agcagaaatc ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga
     2701 ccatgatgtc tcacccccgt atctaacagg ggggtacaaa aacacaaaaa ataagtaagc
     2761 aaccattaag aatgcttgtt gtatcaaaga tagtaagtaa tttgtatagg tgttaactca
     2821 tttgaaaaag ttaacacctt gaatttggag gtgagaactt ggcacaacgg aatttagatt
     2881 atcggctatt aaaagaccga cgcaacgaat acggcgtttc acaaaataaa ttggctacgg
     2941 cttgtggact tagtcggccg tacttaaatc agatagaaaa tggtggcgta accgcatcta
     3001 taaaaaccat gaggaaaatt tttgaccaac tggaaagctt taatcccgat ttgcctttga
     3061 cgttactgtt tgactatgta aggattcgct ttcccacaac ggatgcacga aaaattattc
     3121 aagaaattct ccatttgaaa tttgagtata tgctccatga agattatgcc ttttactcct
     3181 atcaagaaca gtatgtcatg ggtgatattg tggtgatgtt gtctcatgaa gaagataaag
     3241 gtgtgctttt agaattaaag gggcgaggtt gccgacaatt tgaaactttt ttactcgctc
     3301 aaaagcgtag ctggtacgac tttttcgagg attgtctaaa ggccggtggc gtgatgaaac
     3361 gcttggactt ggcaatcaat gatcgagtgg ggctattgga tatacctgag ctaacgaaaa
     3421 aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt taaaagttat cgttccgggg
     3481 aacttttaaa ggctgatgaa aaggacggca tgggcaatac cttatatatc ggcagtctca
     3541 agagtgaggt ttatttctgt ttgtatgaaa aagactatga acaatatatc aagttaggga
     3601 ttccattaga ccaaactgag acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg
     3661 cttttcatgc gattcaagat ttgttaaaag gtcgtagcat tgagagcacc acgttttcca
     3721 ttatcaatcg ttacttgcga tttgcggata aagtagaagg taaaaggcga accaactggc
     3781 ccttgaatga acaatggggg cgttttattg gacggaatcg gaaagagatt caattaactt
     3841 ccgagccaaa accgtatacc attgaacgaa ccttgaattg gctaggccga caagtcgccc
     3901 ctacgtggaa aatggctaag gagttggatc gattgaatcg gacaacctat atccaggata
     3961 tggtacagaa tgcacgctta tcagaccgac ataaaaagat tttggagcaa caaagtatgg
     4021 caattgaaaa tttgattata tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt
     4081 tttggtattt ggtggcagta tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc
     4141 agcagcggat cgagcaatgg aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt
     4201 tatgtgcgac tcgttcctta gtgaaaagct aaggcactaa taaaagaacg aaaaagtttg
     4261 gttattagga gaactgataa tctgacaggt ggaatgatga agtaacgctc tgaaacgcct
     4321 gccctgagcc tccgacaagc attgtatgag gaaactcaga atgtttgaag ctcggtaaag
     4381 acggctgaaa gataccgtaa tagttgatga acatatatct tctatcgaaa actatccaga
     4441 ggtgggtagc gtatccaaca ggtcgggggt ctataaaata tctatggtta gaatgttcta
     4501 taaggaactg acgaaacagc gaatgtacgg gtctaaattt gaactttgta gaaatgcaaa
     4561 ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat aggttatgaa atgccatata
     4621 acgttacagg cggtatcaag ccagcaggct tatagctagt gacctaagga tatatatgta
     4681 tagataagat tatcggaacg aggaaaggta ttgggttctc atcaagagaa taaactgacg
     4741 aagaacaata agcagttgaa ctaatgctga aaagcagagg gcgaacctaa gaaattcttg
     4801 taatgagaat ggaggaatgc cctcaagtcg tgtatatatt agaaaagtat tatgttgttt
     4861 caataaggat tacgagtaag ataaaaaagc tcactcaatt tcaagtaaag gagtgaagcc
     4921 ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga tttaagacac gctgaatatt
     4981 acgaaatgca aaacaccttt gatgatttat atgccaaaag taagaatgga gatattttca
     5041 ctcatcttat ggatattatc ctatcgagag aaaatatatt actggcatat cgaaatatca
     5101 aagcaaatgc aggcagtaaa acagcaggaa cagacggtac aataattaaa gatattggca
     5161 aattgccagc agaaacagtt gtcaaaaaag taagatacat tgttgcaggc agtccacatg
     5221 ggtatcgacc taagccagtg aggcgaaaag aaataccaaa accaaatggt aaaaccagac
     5281 ctctaggtat tccttgtatg tgggacaggc ttatacaaca atgtataaaa caagtattag
     5341 aaccaatctg cgaagctaaa tttagtgaaa acagctatgg ttttagacct aataggtcag
     5401 ttgaaaatgc aataaaagca acctataacc gcctacaaat cagccagcta cattatgtga
     5461 ttgaatttga tattaagggt ttctttgaca acgtgaacca ttccaagctg ataaaacaga
     5521 tatgggctat gggaatacgg gacaagcact tgatattcat tttaaaaagg atattaaaag
     5581 ctccaatcaa aatgactaac ggtacaataa catatccaga gaaaggaact ccgcaagggg
     5641 gaataatatc cccactacta gcaaacattg ttcttaatga gttagaccat tgggtagaaa
     5701 gccaatggca ggaaaatcct gttacaaaaa actatgtggt tcatatcaat aaaagcggta
     5761 gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac aaagttaaag gaaatgtata
     5821 tggttcgtta tgcagatgat tttagagttt tctgccgcta caaggaaagt gctgaaaaag
     5881 ccaaaatagc aatcactcaa tggattgaac aacgattaaa attggaagta tcacaagaaa
     5941 aaacaagaat tgtaaatgtt agaaaaagat actccgactt tcttggtttc aagattaaaa
     6001 tgataccgag aagaaagaag ttagttgtta aatcacatat aagtgataaa caattcaaaa
     6061 accaaaaaga caagctggta tcacaggcaa agaaaattgc ttctccctca aaagggaaaa
     6121 cggaattaga agaaacaaga ctttataatt caatggtatt aggtatgcaa aattattatc
     6181 agattgcaac gaatgtaaat ctggattgta tgaaactgaa tagagctgta atggctatct
     6241 tcactaacag attaggtaca aacaaaaggg gcagattgag aaaaacaggc agacaactta
     6301 cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct tcggtatgtt gctggcgagg
     6361 gcgaacctat tttccctgtt ggatatatcc aacacagaaa accaatggca aaggtgtatt
     6421 tagcgaattg ctacactcta gagggaagaa aatttatcca caccaatcta agtgttgata
     6481 aattactgat gtaccagtta atgaaattat ctttggaaaa tcgcacaata gaatacgcag
     6541 acaatcgaat ttcgctattt tctgctcaac atggaaaatg tgctataact ctggaagaat
     6601 ttcaacaagc taatgaaata cattgtcatc atattgtacc aactggagct ggtggaaatg
     6661 acgattataa aaatcttatt ttggtaaaag aggcggtaca ccgtctaata cacgctaaaa
     6721 cagaagaaac aatacaaaaa tatatagctc tattgaagct aaataaagtc cagctcataa
     6781 gactaaataa atatagagta ttagctggca atcaagaatt aaacttgata taggtctact
     6841 acataaatac ttttcaatat aaacacgatg gaacgcggag tgcggtgaaa gtcgcacgct
     6901 ccgtgtgaat ggggggaaaa gtttgagata tttataatat caggaactta cctatccata
     6961 ta
//
LOCUS       DAFBQW010000107         6833 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFBQW010000107 DAFBQW010000000
VERSION     DAFBQW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.112"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            85..1557
                     /locus_tag="LK889_002784"
     CDS             85..1557
                     /locus_tag="LK889_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5096963.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            1640..2428
                     /locus_tag="LK889_002785"
     CDS             1640..2428
                     /locus_tag="LK889_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK5096964.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(2495..3163)
                     /locus_tag="LK889_002786"
     CDS             complement(2495..3163)
                     /locus_tag="LK889_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBK5096965.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            3278..4990
                     /locus_tag="LK889_002787"
     CDS             3278..4990
                     /locus_tag="LK889_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096966.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4993..6765
                     /locus_tag="LK889_002788"
     CDS             4993..6765
                     /locus_tag="LK889_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096967.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2081 a         1147 c         1482 g         2123 t
ORIGIN      
        1 ttttttatgg cagttaacct cttgaaaaag atagtaatta tttatctatt tttagatcct
       61 gtcataatga aagggaggaa taagatgaaa aaagaaacaa atgttacttt ggtcacagtg
      121 agtattttca ttgcaacgtt catgacggct attgaaggta caatcgtaac aaccgctatg
      181 ccaacgattg tcggttcatt acatggaatg gaaatcatga actgggtatt ttcgatttat
      241 ttgctgacaa acgctatgct tactccgatt tacggaaaac ttgcggataa agtgggacgg
      301 aaaccaatct ttatttttgg gacattattg tttattatcg gttcgttatt ttgcggttta
      361 tctcaaagca tgattgggtt gattgctgct cgtgcgattc aagggatggg tgctggggca
      421 atgatgccag tagctttgac gatcatcggc gatctttatt ctattgaaaa gcgagcaaaa
      481 gttttaggtt tgaatagttc agcctggggg attgccagtg tttttggacc gcttgcggga
      541 ggattcatcg ttgaaacgat cagctggcac tggatctttt ttatcaatgt gccgatcgga
      601 attctattaa ttattttgat ttggctatat ttaaatgaac caaaaatcat acatgagtct
      661 aaaccaatgg atatttttgg tagttttagt ttgatggcca tgcttgtctc attgctgtta
      721 gggttccaat tattaggaga cgaaggaatg agtccgcttg tttctgcttt gctgctaggt
      781 agtatcgtaa tgttaggtat ttttgttttt gccgaaaaaa gagctaaaga tcctgtcatc
      841 atgatgcatt tattcaaaaa tccactattt gttgtggtca acttggctgc agcattggct
      901 agtggttttt tgatgggaat cgatgtttat atccctatgt ggatgcaagg tgtattaggg
      961 ctgaatgcag caattggtgg gctggtatta gcaccattat cactagtctg gatggtcggt
     1021 tctttccttg caagcaagtg gatgactcaa atgaaaactc attgggtctt gagaattgga
     1081 ctactaatcg cagctattgg gggcgcttgg ttgtatctta tgcctttcag aagtaactat
     1141 ttatggtttt ttgctatatc aacggtcatc ggtataggct taggtgtgac catgactacc
     1201 tcgactgtca gtgcacaaaa tagtgtatct cgtgaccagc ttggtgtagc aacttctttt
     1261 aatacgttag ttcgaacaat tggtcaaaca gtcatggttt ctatttttgg tttattgata
     1321 aatggggtaa ctaaacgaga acttgcagca gcaaatttaa caaatgatgc agatatcatg
     1381 aataaactgg tgaatccaca aacggctaaa tatattgatg cggatatttt gttaccatta
     1441 agaaaaattc tttatgatgg gttacattcc gtttatctag ttggcttgtt actcgtgatc
     1501 attgctttgg tcttaacgct tttcgttcat gaaaaaagtg gtaaaataaa acagtagttt
     1561 cggttaagat atgtacgtag ccgaatcatt caaatttaaa gttattcaaa aataacttct
     1621 tactaaggaa ggcgaaaata tggcatttat tcaagcaaat atttattcga atgtgttaga
     1681 aatggaagtc aatctaaatg tgatactacc gcaaacaaca aaaaaggtga ttggcaccag
     1741 ttcagagcaa gagacaacgg atatacctgt tttatattta cttcatggaa tgggtggcaa
     1801 tcatagtgta tgggaaagac gtacgtcgat tgaacgttat gtagcaaact acggattagc
     1861 tgtcatcatg ccatctactg atcttggctg gtacacagat acagtttatg acatgaagta
     1921 ttggacattc attgcagaag agctacctga gatttgtcat gaattattcc cgcaattaac
     1981 aagaaaaaga gaaaaaacat atgccgctgg attatcgatg ggcggatatg gcgcgttaaa
     2041 actaggcctt gcaaaatcag aaaatttcgc agcagtaatc tctctttcag gcgctgtttc
     2101 tgtaggaaat cgaatggatg atcttttgat ggttcgtaaa gcatcttttt gggaagggat
     2161 ctttggacca ttggatcaaa tcgaaggttc aaaaaatgat ccgcaatact tattgaaaga
     2221 actagtaaaa agcggaaaac cagttccaag attttatatg gcttgtgggg aatctgattt
     2281 tctttatcag gcaaatcagg aaatggtatc tgctatgcag gaacaaggct tggcggtcac
     2341 ctttgaacaa ggacctggcg atcataactg gatcttctgg gaccaatgga ttcaacgtgc
     2401 attggcgtgg ctaatggaag aaaaataaaa aaagagtctt aaaaagatag ctcctataga
     2461 gatcacaaac atagtgatct ctataggagc ttttttactg ctcgttttct cttcttttat
     2521 tttgttggca taagccttca atataggttt gtgcactcgt tagaaagagt gctttttcat
     2581 tcaattctgc atgggactgc atactttctt taaatccctc taattgattt tcaactacat
     2641 aattacgcat aaatagaact gtttctttca aaaattcatt tccgtccata attttttgtc
     2701 gaagctgctg ttcatgctgc tgatccatta actgtccaat caataaattg tggagagagg
     2761 caatggcctg atgactttgg gtgactgaaa atccttcacg aacaagtaat tggatcattt
     2821 gctccaaatg ttctaatcga ctcggaaaag cgggcacagt attgatttct aattctgccc
     2881 cacaagggaa acgtgtataa aggtcatagt aattttccat atactgcagc aactgttcat
     2941 tccaaggcaa agttggatct ggaagcgcta attcttcttc cattgtttca gccatttttt
     3001 gcaagagctg ttgtttgttt ttgaaatacc aatataaagc aggtgcttga acatgaattc
     3061 tgtttgcgat gtttcgcatg gaaagctgcg aaagagcttg tgtttcagcc aggatatcaa
     3121 aggcggcctg aatgatgatt gcttgagaaa gctttatttc cattttatca acctcgtgta
     3181 ttgcattatc tttttaaaca tggtaaattg ttagtcggtc taattttaca gtgttaaatt
     3241 agtcctgtca atttaaacaa aggggtgtaa tcaacgaatg aaattaatct tacgacatgc
     3301 gaaaaaatat aaagtagctg tttttatttc cttgctgtcc gtagcagtca tggttgcagc
     3361 agcattatgg caacctaagt tactacagca agtattggaa gctattatta cagaagataa
     3421 tgatgaaatg aaaacaattg gtatttacct gatcagtctg gctttgctag gattagccgc
     3481 aggtgtaacg aacacgattt tctctgcaaa agtagctcaa ggcgtaagtg cagatatccg
     3541 agaagaagca tttcgaaaga tccagacatt ttcatttgga aatatcgaac agttctctgc
     3601 tggaaatctt gttgttcgtc taacaaatga tatcacgcaa atccaaaatt tagtgatgat
     3661 ttctttgcaa tcactttttc gaattccatt cttatttatt ggtgccttca ttttagctat
     3721 gatcacgatg ccgcaattat ggtggatcat cgtagcgctt atcgttacgg tatttctgat
     3781 tacggcactt tcatttacaa ggatgggcaa gcacttcatg atcattcaaa aactgattga
     3841 tcgtgtgaat agcattgcaa aagaaaactt actagggatt cgtgtagtga aatcttttgt
     3901 acaagaagaa aaccaattga agtcattcag caaagtaagt gaggacttaa caaaacacaa
     3961 catcatagtc ggtacattat tttctgtgat gatcccgtcc ttcatgctgg ctgccaattt
     4021 ggctgtggtt ggggcgatct tttttgtgag cgatcttgtt aaagatgatc ctacgttaat
     4081 tggtggaatt gcttcattca tgaactactt gatgcagatt atgatggcaa tcattattgg
     4141 cggaatgatg atgatgatga cttcaagagc agccgtatcg ctgaagcgga ttgcggaaat
     4201 tttagatgct gaaccagatc ttacttattt agacgttcca gaacaagagt tgacgggttc
     4261 tgtcactttt gaccatgttt cttttcgtta tcctggtgag gatacagata cgcttaaaga
     4321 tatcagcttt tctatcaaac ctggagaaat ggttggtatc gtcggggcga ctggtgccgg
     4381 gaagtcaaca ctagcacaat tgatccctcg tctatttgat ccgacagaag gcaaagttga
     4441 agtaggtggt gtagatcttc gtcaagtaaa tgaaaagagt ttaagacaaa cggtttcctt
     4501 tgttttgcaa aaagcaattt tattctcagg aacgattgct caaaacttgc gccaagggaa
     4561 aaagaatgca acagaaaaag agatggaaca tgcttcttct attgctcaag caaaagaatt
     4621 catcgaaaag ttatctgatc gttacgaagc accaatagaa gaacgtagca gtaacttttc
     4681 tggtggtcaa aagcaacgtt tatccattac tcgtggcgtt atcggtaatc caaagatctt
     4741 gatcctagat gacagtacga gtgcactgga tgctcgctct gaaaagctgg tccgagaagc
     4801 actcgatcga gatttgaagg gaacaacgac gattgtcatc gcacaaaaaa ttgcttctgt
     4861 tgtcaaagca gaccgcatct tagtattgga tgaaggcaga ttagtcggcg aaggaaccca
     4921 tgaggaattg gtagctggaa accgtgtcta tcaggaaatc tttgaaactc aaaaaggaac
     4981 ggaggagtaa gtatgactga tctaataaaa gcaagtaaat ttttctatca ctatttgaag
     5041 cggtacaaac tctccttttt ctttatcttt atcacagtga tcattgctac ctatctacaa
     5101 gtaaaggcac ctcaatacat cggggaagct tttcaagaat tagctaatta cgtaggaggc
     5161 ttgatgcaag gcgtagacga taaaagcaaa tttgtttctg taatatggaa actcttgctg
     5221 ttttatgtac tagctagtgc agccaacttt atctatagta ttttgttcac acaagtagta
     5281 gggaagtcaa cgaaccgaat gcgtatcggt ctattcaaca aattggaaaa attgacgatc
     5341 cgtttctttg attctcatca agacggtgaa attttaagtc gttttaccag tgacttagat
     5401 aatattcaaa atagtttgaa ccaagctttg cttcaagtca tcacgaatgc tgttttactt
     5461 gtcgggatct tgatcatgat gttccgccaa aatgtagagt tagcttgggc tacaattgct
     5521 tccactccag tagccgtttt gatagctgtc ttgattatcc gcaaagcacg gaaatatgtg
     5581 gatcttcagc aagatgaagt ggggaaacta aatggttata tggatgaaaa aatcagcgga
     5641 caacgtgtca tcatcacaaa tggcttacaa gaagaaacga ttgaaggatt cttagagcat
     5701 aatcaaagtg ttcgcgaagc aaccttcaaa ggacaagctt attctggtct actatttccg
     5761 atgatgcaag gaatgtcact ggtcaataca gcgatagtta tctttttcgg cggctggtta
     5821 gcattaaatg gtgatttgga acgttcggtg gcattaggct tagttgtgac attcgtccag
     5881 tattcacaac aatattacca accactgatg cagatttcat ctggatacag catgatccaa
     5941 ttagcgatta caggggcaag acgattgaat gaaatatttg atgaaaaaga tgaagtcaaa
     6001 ccagaaaacg gtaaagcatt tgatggtgtt gaaaaaggtc ttgcgttgca gcacgtagat
     6061 tttggttatt ctccagaaaa actagtcttg aaagatgtct cgatcaatgt ggacaaagga
     6121 gaaatggttg ctcttgttgg tccaacagga tcggggaaaa cgacgatcat gaatctgcta
     6181 aaccgttttt acgatgtcaa tgatggttca gtagtcatcg atggaataga tatccgagat
     6241 tatgatcttg atgcattacg tagacatgta ggaatcgttt tgcaagaatc tgtattgttc
     6301 tccggaacga tcaaagacaa tatcacattc ggcaaaccgg acgcttcaga tgaagaagtc
     6361 atcaatgcag caaaacaagc gaatattcat gacttcatca tgacgttaga aaaaggttat
     6421 gatacagaga tatccgaaga aaataactta ttcagtacag gtcaaaagca gttgatcagt
     6481 attgctcgaa cgatcatcac aaatccatct ttattgattt tggatgaagc aacaagtaat
     6541 gtagacacgg taacagaagc aaggatccaa aaagcaatgg atgaagcaat caaaggaaga
     6601 accagctttg tgattgctca ccggctgaaa acaatcttga atgcagatag aatcgttgtc
     6661 ttgcgtgatg gggaagtcat tgaagaaggc agtcatcatg agttattagc agaaaatgga
     6721 ttttatgcgg aactttatca taatcaattt gtatttgaaa gttaaattaa aagaacctcg
     6781 ttataacggg gttcttttta tatattttat gttgtttgtt tttataaggg gca
//
LOCUS       DAFBQW010000108         6643 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFBQW010000108 DAFBQW010000000
VERSION     DAFBQW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.113"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(1..859)
                     /locus_tag="LK889_002789"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LK889_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LK889_002790"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LK889_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK5096968.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LK889_002791"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LK889_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="LK889_002792"
     CDS             complement(2143..2373)
                     /locus_tag="LK889_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBK5096969.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="LK889_002793"
     CDS             complement(2603..3421)
                     /locus_tag="LK889_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5096970.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LK889_002794"
     CDS             3582..4271
                     /locus_tag="LK889_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK5096971.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LK889_002795"
     CDS             4285..5787
                     /locus_tag="LK889_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5096972.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LK889_002796"
     CDS             5800..6276
                     /locus_tag="LK889_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5096973.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LK889_002797"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LK889_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAFBQW010000109         6639 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFBQW010000109 DAFBQW010000000
VERSION     DAFBQW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.114"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(1273..1938)
                     /locus_tag="LK889_002798"
     CDS             complement(1273..1938)
                     /locus_tag="LK889_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5096974.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="LK889_002799"
     CDS             2118..4100
                     /locus_tag="LK889_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBK5096975.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="LK889_002800"
     CDS             4104..4742
                     /locus_tag="LK889_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5096976.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="LK889_002801"
     CDS             4770..5198
                     /locus_tag="LK889_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096977.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="LK889_002802"
     CDS             5655..6566
                     /locus_tag="LK889_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5096978.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADNGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2286 a         1005 c         1220 g         2128 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataatggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaa
//
LOCUS       DAFBQW010000110         6460 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFBQW010000110 DAFBQW010000000
VERSION     DAFBQW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.115"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(105..560)
                     /locus_tag="LK889_002803"
     CDS             complement(105..560)
                     /locus_tag="LK889_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096979.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="LK889_002804"
     CDS             complement(714..1922)
                     /locus_tag="LK889_002804"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBK5096980.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="LK889_002805"
     CDS             complement(2034..2366)
                     /locus_tag="LK889_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK5096981.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="LK889_002806"
     CDS             2514..3389
                     /locus_tag="LK889_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5096982.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="LK889_002807"
     CDS             complement(3471..4052)
                     /locus_tag="LK889_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBK5096983.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="LK889_002808"
     CDS             complement(4058..5317)
                     /locus_tag="LK889_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK5096984.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="LK889_002809"
     CDS             complement(5319..6365)
                     /locus_tag="LK889_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096985.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1797 a         1338 c         1102 g         2223 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcacttgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata
//
LOCUS       DAFBQW010000111         6399 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFBQW010000111 DAFBQW010000000
VERSION     DAFBQW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.116"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..142)
                     /locus_tag="LK889_002810"
     CDS             complement(<1..142)
                     /locus_tag="LK889_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5096986.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="LK889_002811"
     CDS             complement(183..437)
                     /locus_tag="LK889_002811"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK5096987.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="LK889_002812"
     CDS             complement(548..802)
                     /locus_tag="LK889_002812"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5096988.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="LK889_002813"
     CDS             complement(995..1270)
                     /locus_tag="LK889_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBK5096989.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="LK889_002814"
     CDS             complement(1242..2195)
                     /locus_tag="LK889_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5096990.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="LK889_002815"
     CDS             2807..4300
                     /locus_tag="LK889_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBK5096991.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="LK889_002816"
     CDS             4435..4731
                     /locus_tag="LK889_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBK5096992.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="LK889_002817"
     CDS             complement(4755..5714)
                     /locus_tag="LK889_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5096993.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..>6399
                     /locus_tag="LK889_002818"
     CDS             5897..>6399
                     /locus_tag="LK889_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096994.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVST"
BASE COUNT         2046 a         1080 c         1125 g         2148 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagcaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaa
//
LOCUS       DAFBQW010000112         6286 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFBQW010000112 DAFBQW010000000
VERSION     DAFBQW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.117"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..177
                     /locus_tag="LK889_002819"
     CDS             <1..177
                     /locus_tag="LK889_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5096995.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="LK889_002820"
     CDS             190..690
                     /locus_tag="LK889_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK5096996.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="LK889_002821"
     CDS             787..2634
                     /locus_tag="LK889_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBK5096997.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="LK889_002822"
     CDS             2637..3533
                     /locus_tag="LK889_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5096998.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="LK889_002823"
     CDS             3583..3972
                     /locus_tag="LK889_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5096999.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6286
                     /locus_tag="LK889_002824"
     CDS             3959..>6286
                     /locus_tag="LK889_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5097000.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1887 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagaggcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaac
//
LOCUS       DAFBQW010000113         6173 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFBQW010000113 DAFBQW010000000
VERSION     DAFBQW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.118"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            725..811
                     /locus_tag="LK889_002825"
     CDS             725..811
                     /locus_tag="LK889_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK5097001.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="LK889_002826"
     CDS             complement(932..1621)
                     /locus_tag="LK889_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097002.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="LK889_002827"
     CDS             complement(1628..1906)
                     /locus_tag="LK889_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097003.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="LK889_002828"
     CDS             complement(1866..2780)
                     /locus_tag="LK889_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK5097004.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="LK889_002829"
     CDS             complement(2762..3142)
                     /locus_tag="LK889_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK5097005.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="LK889_002830"
     CDS             complement(3592..3879)
                     /locus_tag="LK889_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK5097006.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="LK889_002831"
     CDS             complement(3899..4123)
                     /locus_tag="LK889_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBK5097007.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="LK889_002832"
     CDS             complement(4578..4748)
                     /locus_tag="LK889_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097008.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="LK889_002833"
     CDS             complement(4763..5296)
                     /locus_tag="LK889_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK5097009.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="LK889_002834"
     CDS             complement(5317..6159)
                     /locus_tag="LK889_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK5097010.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAFBQW010000114         6068 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFBQW010000114 DAFBQW010000000
VERSION     DAFBQW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.119"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            832..1566
                     /locus_tag="LK889_002835"
     CDS             832..1566
                     /locus_tag="LK889_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5097011.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="LK889_002836"
     CDS             1582..2751
                     /locus_tag="LK889_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5097012.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="LK889_002837"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="LK889_002837"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK5097013.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="LK889_002838"
     CDS             3841..4767
                     /locus_tag="LK889_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBK5097014.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="LK889_002839"
     CDS             4786..6036
                     /locus_tag="LK889_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5097015.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAFBQW010000115         5877 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFBQW010000115 DAFBQW010000000
VERSION     DAFBQW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.120"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(126..772)
                     /locus_tag="LK889_002840"
                     /pseudo
     CDS             complement(126..772)
                     /locus_tag="LK889_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(1180..1683)
                     /locus_tag="LK889_002841"
     CDS             complement(1180..1683)
                     /locus_tag="LK889_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5097016.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1806..2570)
                     /locus_tag="LK889_002842"
     CDS             complement(1806..2570)
                     /locus_tag="LK889_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBK5097017.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2678..2953
                     /locus_tag="LK889_002843"
     CDS             2678..2953
                     /locus_tag="LK889_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK5097018.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3391..4341
                     /gene="yidC"
                     /locus_tag="LK889_002844"
     CDS             3391..4341
                     /gene="yidC"
                     /locus_tag="LK889_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK5097019.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4520..4720)
                     /locus_tag="LK889_002845"
     CDS             complement(4520..4720)
                     /locus_tag="LK889_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097020.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            5525..>5877
                     /locus_tag="LK889_002846"
     CDS             5525..>5877
                     /locus_tag="LK889_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5097021.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIF"
BASE COUNT         1758 a         1205 c         1027 g         1887 t
ORIGIN      
        1 aaaggggctg tccccagact ttagtaggcg tcattagcta tctttcgtta gcatttaaag
       61 gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa
      121 taaaattatc gttctttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt
      181 aactgttgat tacattggtc actatcatga caaatataat tccccttttt cgtatacgtg
      241 ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag
      301 attgcacaga ttcccttatt tatcgttggt gataaagtac cgctaatccc ttttaatttt
      361 tcatcttcgt aatagaccat gaattttttc tgcgtacctg gatcattcca gccgatgtac
      421 gtatgttctt gcaagtcgat ttcattccaa gctggttgtt tgagtttttt cacttttcgg
      481 aataatttct cgatttgtgg tttgctaggc tgcataaacg gctgaacgat ttgcttcagc
      541 tcctccaatg ctctttctgc ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa
      601 aatgtatcga ttctggcagt tcgtgatcta atatgtctcg aatcttttca gctgctagtg
      661 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
      721 ctacttctct tattataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
      781 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
      841 gtgttacctg aagatcccgg actcttttaa ataagatacg ttttgcatct tatcatcacc
      901 ccctttaatg cttattattc tatgtacatg attcattcga taaataaaag taaaccgtat
      961 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaaagtc gtgtcaaagt
     1021 cccttgtttc gaatgaatgg tgttcaaaag ctggcatctg cggtaataag ccgaaacttc
     1081 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     1141 tgtcacaacc tcaatatggt ctattattgg ttttatctat taaatcgagg attctttccc
     1201 aaagtaacgt tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac
     1261 tgcgcagtat ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat
     1321 cgttgcgccc cacatgtgct tcaaaatgat atctcgctca agatccgaca attcggtgat
     1381 cttttctgca ttctttacag caattcttgg atggatatac gcatgtgtgc cttcatcaaa
     1441 tttcgtcgta cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc
     1501 tttagcattg ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga
     1561 atgctccaaa cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc
     1621 tgtttctaaa agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt
     1681 cataattatc gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat
     1741 accaccagtg ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc
     1801 cttaatcata aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat
     1861 tccgcatttc ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga
     1921 cttaccccct gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg
     1981 tctaatgcgt gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc
     2041 attaaaggaa tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc
     2101 tgcatgctgc gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca
     2161 ccagccatat caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt
     2221 acattatcta acaacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc
     2281 atcactgcta agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc
     2341 gaaactagaa aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg
     2401 cctcgctgtg tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc
     2461 agatggaacc cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt
     2521 ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc
     2581 tccccatgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     2641 tcattaattt ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt
     2701 ccaaggacgc gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact
     2761 aggcgtgact ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg
     2821 caatgacgac atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg
     2881 acgagtgcag tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga
     2941 tgtggtggga taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga
     3001 aaaatagctc tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga
     3061 acaccgtcga taaggttatt cttgaggcgc tttgcctcaa aaattaagga cagcagtgaa
     3121 aaagctgatc tatcacttgc agtgagttca tttgactccg ctttttcggg cagaaaaaaa
     3181 gtttttagtt tattaaagta agaaagaaac acttggaatc attctacttt ttcctagtat
     3241 ttctttcttg attttttgtc tttttttcaa aatttttcgc tacgtaaaag aacgaatatt
     3301 tatatgaaat ttctatgaag ttgattgaat tccatgcgct tttttagtat agttaagttt
     3361 gtgtaaaaat tatagaagag gaagtattct atgaataaat ggaaaaactg gttattggga
     3421 tccggacttg tcagtatcct gctcttttta tctggctgtg tgcggatgga ttcaaacgga
     3481 aaccctgaca cttcgggaat cgtttatcga gtactcgtcc atcctatggg ccaagcgatt
     3541 acatatctag ttgaaaattt caattggtct tatggctggg cagttattgc aatgactgtg
     3601 atcgtccgta tcatcatctt gccactaggt attagtcaat cgaaaaaaac aatgatccaa
     3661 tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag cccaacaaaa attgaaagct
     3721 gcaacaacac gtgaagaaca aatggcagca caagcagaaa tgcagcaagt ataccgcgaa
     3781 aacggattga gtatgaccgg cggaatcggc tgtctgcctc tattgatcca aatgccgatc
     3841 ttctctgcct tgtactttac ggcacgttat acagaaggta tccgggaatc atctttctac
     3901 ggcatcgatt taggctcacc aagtatggta cttgttgcca ttgcaggggt cgcttatctg
     3961 cttcaaggat acatctcaac gatcggtatt cctgaagaac agaaaaagac gatgcgtacg
     4021 atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga tcagtgcgcc agccggtgtt
     4081 acactttact gggtagtcgg tggtgtgttc agctgtctgc aaacattcat cacaaacgtt
     4141 atgatgaaac cacgtttgaa agcacaagtg gcagaagagt tgaaacaaaa cccaccaaaa
     4201 caagtcgtca ctccaagaat tgatgtgaca gaatcagcta aaccagctga cagcaaacaa
     4261 gcaaccaaac aaatcaagca acccaaaaac agtacaaaca ataaaggccg caacgcagga
     4321 aagcagcaga agcggaagta gatagtaaat aagagtgtaa taaaaattca ttaacagttt
     4381 tagcaattaa aaatatcgac caagattttt gaaaaagaaa tctggtcgat atttttattt
     4441 aagtgaatgt ttaatttgaa tcatcgattg atcgctatct aattgcagca gcatcttttt
     4501 atgaagaaac aactttttct tatctatcaa cgtagacagg tgttgctgtt tcttctaata
     4561 gtgtacttaa ttccttaata ttttgttttc cttcttctaa catccaacta ctcgcttttt
     4621 cttcgccttc ggtaacaaaa ggcgccactc ccttcgccca tgttgctcgc ccacataaga
     4681 caccattctc cataaaggac aacgcggaga taccacccat ctaaataaca tccataaacc
     4741 tttctagtag aagaatagtg atagtatcca tccctttttc tactgggaat acaattacac
     4801 gattcaaagc ttgatacaca acatttccct tcttataaac cgctattgta accgaattgt
     4861 tttgaatcac ctttttggga tagaaactgg ctcctttccg caacttctta aatgttggtt
     4921 tcttttatac tttctttgta gcaactacaa atttcttcaa atctctaaat gtttgcataa
     4981 caaccgcctt tctgactttt actcaggatg gtatttaaaa atcagcaaaa taaggcttct
     5041 ctccatcttt tttatttcct agctgcttta aagacactct tgctgttttt taatctctat
     5101 caagggggct ctgaatctat tttgatgaac ttatcttatt aatttctcaa atgagctgtc
     5161 agacacaact gtattttttc aatctcatac cggacaacta tgccagctta aaaaaatttc
     5221 ctctggcaaa acttctttat tgaaaaatga caatgaaaaa agaccattct ttttgttgaa
     5281 tgctcattgt gtaaacatat ttcctgcgac tgctcttcga accattctca gtgcaatccc
     5341 taatttatct attaaatctt cttttagttc ctcatctttc atattcaaaa tttcctttgt
     5401 atgttacaca agtttgttct ctaaataacg cgaattgtaa aattaagtta gaaaaataaa
     5461 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
     5521 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
     5581 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
     5641 taatgtagtc aataagttca agcaaggtaa gactgctctt gattattggc accagtataa
     5701 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
     5761 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
     5821 gcctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa tatttga
//
LOCUS       DAFBQW010000116         5467 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFBQW010000116 DAFBQW010000000
VERSION     DAFBQW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.121"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            119..1108
                     /locus_tag="LK889_002847"
     CDS             119..1108
                     /locus_tag="LK889_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5097022.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="LK889_002848"
     CDS             1090..1551
                     /locus_tag="LK889_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK5097023.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="LK889_002849"
     CDS             1601..2065
                     /locus_tag="LK889_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5097024.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="LK889_002850"
     CDS             2079..2828
                     /locus_tag="LK889_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5097025.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="LK889_002851"
     CDS             2828..3658
                     /locus_tag="LK889_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5097026.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="LK889_002852"
     CDS             3658..4305
                     /locus_tag="LK889_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5097027.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="LK889_002853"
     CDS             complement(4403..5179)
                     /locus_tag="LK889_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5097028.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5261..5404
                     /locus_tag="LK889_002854"
                     /pseudo
     CDS             5261..5404
                     /locus_tag="LK889_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          740 c          973 g         1882 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa ttttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtatcgtaaa gttatataac tttataatac ctattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtattaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgtatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttcataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgactcc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ttaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaga tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaata aaagattaag atataaatat aaatgaacta gtctttgact aaccaatgcc
     5281 cgcttttgaa agaaaacaaa ttcattctgt tttaagtaaa aatccttatg ttaccactca
     5341 ttctgaaggg gatgagcctt atcgctactt ggttgtggaa ccaagcgata agacaatttt
     5401 ataaattcaa tattaattaa aataaaaaca aacaaagtag attattttac tttgtttgtt
     5461 tttttat
//
LOCUS       DAFBQW010000117         5419 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFBQW010000117 DAFBQW010000000
VERSION     DAFBQW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.122"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..191)
                     /locus_tag="LK889_002855"
     CDS             complement(<1..191)
                     /locus_tag="LK889_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097029.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGT"
     gene            complement(188..349)
                     /locus_tag="LK889_002856"
     CDS             complement(188..349)
                     /locus_tag="LK889_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK5097030.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(364..723)
                     /locus_tag="LK889_002857"
     CDS             complement(364..723)
                     /locus_tag="LK889_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBK5097031.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(720..1571)
                     /locus_tag="LK889_002858"
     CDS             complement(720..1571)
                     /locus_tag="LK889_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5097032.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1588..2418)
                     /locus_tag="LK889_002859"
     CDS             complement(1588..2418)
                     /locus_tag="LK889_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBK5097033.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2421..3107)
                     /locus_tag="LK889_002860"
     CDS             complement(2421..3107)
                     /locus_tag="LK889_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097034.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3113..3352)
                     /locus_tag="LK889_002861"
     CDS             complement(3113..3352)
                     /locus_tag="LK889_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097035.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(3678..3866)
                     /locus_tag="LK889_002862"
     CDS             complement(3678..3866)
                     /locus_tag="LK889_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097036.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(3887..4171)
                     /locus_tag="LK889_002863"
     CDS             complement(3887..4171)
                     /locus_tag="LK889_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097037.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(4173..4361)
                     /locus_tag="LK889_002864"
     CDS             complement(4173..4361)
                     /locus_tag="LK889_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5097038.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            complement(4364..5140)
                     /locus_tag="LK889_002865"
     CDS             complement(4364..5140)
                     /locus_tag="LK889_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBK5097039.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            5197..5418
                     /locus_tag="LK889_002866"
     CDS             5197..5418
                     /locus_tag="LK889_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097040.1"
                     /translation="MEEFNMDVDALFKQTVFKTVNKEFKIDFQNDESFPTEIELFEEL
                     SQSVSESFSRQLQKNFFDALVDEFHQQQH"
BASE COUNT         1349 a         1126 c          864 g         2080 t
ORIGIN      
        1 gtacctgtta ctagaattcg atcaccatgc cagattgcaa tttttctcgg cgttttccgt
       61 ttaggctttt cagtccacat tgatttaccg agcttttgga cttctgcaac tatttctttg
      121 tcttcctgcc aagattctga cttggttaat tcagcaattc gtttcattgt cgctttctta
      181 tccacgctca ttcctccaat ctacgaattt cccttcttaa gttctctatg tgcaaatcga
      241 ttgcctttct cgccgtttca ttgaccatca ctgcctttgt tcgttctaga tcgtcaattt
      301 cacgctgaat gtttcgaata cgcatttgaa tcacttcttc tgttgtcatg atggaccacc
      361 tcgttaaaaa cgctcttcct tgaacgtatt ccgatatttt ttagctaata tcaacggcgc
      421 ttgatattga tgacagaaca actttgcctt gatcttaaag tcttttgtct gcattccttt
      481 gacatctacg actttgacaa gtttgccgtt tttataaaat gtgaagtcgg gaatatactc
      541 gatcttgcga tacttctttc cgtctagttc aaatttcggc atcagctcaa atctttcctg
      601 aagttttact ttccagccat ttgcttcagc ttgccacaag gctagatcgt aatactctgc
      661 ttctgcgata gaatcaaact tgatacctcg atgaacagtt tttttattac ggtatttatt
      721 catgcgatac tacctttcac tggttttatg cgcttgtctg ctgtttgttg gaatttcagc
      781 gcataacctt ctgaattctt aaatatccta gaaacaattc tttcaccgta ggcttctctt
      841 agttcaggac cagataagtt tgttgtgatg atcgttgcct tgttctgtct ggcttctaag
      901 agcgtgttta acgtgttgtt tgtaaactgc ctactatttg ataccccact acctaattca
      961 gctccaatat cgtcaaaaac caccaaatca gttgttttaa tatcggctat aagcgatcct
     1021 tcaatttctt ttctcagttc agtattgtta taagaaaact ttatttgctc taataattct
     1081 tgatagctta taaaaagtat tttcttgtca taatttgagc gctcaagtat ttcccaagct
     1141 gtggccattg ataagtggct ttttccgctt cctgatttcc ctgatagaat gaaatgtgca
     1201 ggatggttca gcagaacctc attcgtatag ctttttgctt tttctaaagc gatttttgtt
     1261 tcttgatcca ctacgtgata attttccatt ttgcatttaa acaaagtttt atctgttaat
     1321 accgaaccat tttgaaaaaa actcaacgct cgtgctttta agctatcgtt atatatccgt
     1381 tcggtctgta tatcctcttt cacacgtaac gctttataac cacaactcat gcatgttggt
     1441 ttacaacgtt ctgaaccatc cttattttta gctcgccaac tatacaaagg ttcgctacat
     1501 tctggacatt ttccgctttg cactaatact cttcttatta gtttctccat agcatttgct
     1561 aggctttcca tgtgatgcat ctccttttta aattggcaag tcgtcatatt cactaggatt
     1621 gctgtactgt agtttttgac tttgcttttt atgattcttc ttgtctgctt tgatttcgaa
     1681 tttgagcttc tcaaattttt ctctcaattt cttagcactt ctaatatttc caaaccaaaa
     1741 ttcatttgta ggtagccaat tgatcacata ctcaatcgct tctatagatg ctttatctct
     1801 ttcttccatc aacctgattg tgtctgccca tttttcgata tctactttgt tcatttcttt
     1861 tggaaaatct tcagttaaat tactttgcaa ctttttagca aggcgtaagt gttcgtcaga
     1921 atacttacct ttcttttctt ctttatctat atctttatct tcttctatat ctttatctgt
     1981 accgtcacgt gacgtcacgc taacgtcatt ttccaatttg agacgttcct gtctctttct
     2041 ttccctgtat ttacggtttc tttcagcatt ttttagcctt actttatcca taccctcgat
     2101 attttgatgt ttttcccaat tactgatggc aattagtcca tcactgctta gatcaatcat
     2161 gttgaaattt gccaatgtag ttagcgctaa gcgaaccgta tttacgtttt tgccaaacaa
     2221 tgtagcaagc atttcttcgg tataaggcat gttcctctgg atatatatca gaccatcgtc
     2281 gttagtcttt cctgctaaaa ctagtaatcg aatccatata acgatgatgg catccgactc
     2341 aggaacagct tggattaatc gtattttttc atcgtcaaac atagtagttt taagtttgat
     2401 ccaacttatc tcagccaaat ttatcctcct atccttaact ttttaattgt ttcctggttt
     2461 aacttgatcc ctttgatttg atatttattt ttgaaattga tcacacctat tttgtgtttc
     2521 tctgtgtgat ggattctgca gagtgctgca aatgtgtact ctgaatgatc aacttctttg
     2581 cgctttcgtc ttcccagcgc tttgtcaaag tgatcgatat cagctcctgt tttgccacag
     2641 atgcagcaga ctctttttgt aatgcatttg tagaagtaat actcttgatt cgctggtaaa
     2701 atctcatatc cttctttgaa aggaatatga tgttcaaaga tgaaatctag gatgatattc
     2761 gctaagatat tggcatcact cacggttgta ttcgattcgt ctttgaggct tatttcgcgc
     2821 cctgtgacac cttcaaaacg gaagtagaag aattccttcc agaagtccgt tggcatgcct
     2881 gtatcgatga aaatatcgcc tatgagcgca tagatgaagt ttctttgctg tactgtgaac
     2941 cgtcgtggat caataaagcg aatttcaatg actcgatcgc catcatatcc gtcatacatc
     3001 gttttcagcc gttcgatatt tacttcttcg ttgattgttg caccaatgtc gttaccttta
     3061 aatttcttta gtaccgccga gtatgaatct attagtggtt taaacactca tatcacttct
     3121 cttttgtttc ttctctgtac tgatcttcaa gccaattaac gcctcgtttt agaatgccca
     3181 agtctctctt ggtccattta ctgtcatcag cggttatgga agctgcatca gtcaatgcaa
     3241 caattgcttc atcaattgat ttttcgtact tgttagcaac cagttgtaaa ttcgatttgc
     3301 cccaatcagg atcggccaaa tattgatcta gagtggaaag ttcattttcc atatgttaaa
     3361 atctccttag ttatgatttt ttgagtgact cattgctttg gtcggcggag tcactttttt
     3421 atttgttgcc atgctttttt cttttcgata tgttgcttgc ttaaaataat agaacggcta
     3481 tttgcccacc aattatcagc aatcacttta ccgattttta gcgcttcttc tcgtgccata
     3541 gttgctcctt tcttttgagt caagcagatt gattaaaacc atcaatgctg cgaacaagct
     3601 tcctccgata atactttggt gcgctacaat cactaataac cccaaaatga atcctataaa
     3661 aagtgtgtct gtcttcttca taatctaatc tccctatttt ttatttctag cattctcaaa
     3721 tcctcaagtt cagaagcaat tagttcagct tgtctatctg atagctcatc ggattttcta
     3781 agcgctgcac gatcatcttg taattgtttt ctacgctgtt taatcagacc tagaatttga
     3841 tgttcttgtt gcaatgtgta ggacataaaa tcattctcct ttgcctttag aactcaaagt
     3901 tttctttcaa aaatctttgg agttccgatc gttcaattct gatgtctaac ttgctccact
     3961 gctgtgtttt taagcctagg cttatccaat gtgttaattt gtcatcacca atgcctaaaa
     4021 ctttttttac ctctgatttg tttggatatg gaggaagctc tactgatttg ttcataaggt
     4081 gtaatcgttc gtccaatgaa ttaagaacag cattcgtaat ctgtgtagtt aattcggaaa
     4141 ctactaaatt atctggaatt gttatttgca tgttatttct ccttctctaa ctctgctagt
     4201 gctgcctcaa ttggcttgat ttgtttatct ggttttctac gcccattcat aatgtccgac
     4261 atatatggtc tagaaattcc gatagtatca gctaaccagt tctgagactt tccgtatttt
     4321 gctaattgaa ttctgacttt caaaataaat tcttgtgaca tcgtcattcc tcctctaata
     4381 aatcgatttc tggaatatat ccttcttttt tcagcgactc ataaatgaac agacgccctt
     4441 tttgagtcca ttttgtattc atcacaactt ttgttccgcc atcagatttc ggaatctcag
     4501 ttgtatgaga ttttgtgtaa ccctgtctca tgtgtttttt gcataataac cattggttgc
     4561 ctactttttt ctgaatacct agcttatgaa gtagtttgtt catctgttgt ggagacatcc
     4621 cataatcagc cgcgatttga ctaatagtta ctgaatctgt agaagataag atactatcca
     4681 aatacgaaat tttgggttcg tattctgcaa ttttttgttc tgcgattagt cttccagtac
     4741 gttcttcttt caacttagtt gctaattgga tgattgtatc tggattaagc aaagcttctt
     4801 ctactttttc tggagttaga taacctccat gttttctaat tgctggcaac acttcgctag
     4861 tcacccaacg tttaaatttc ttagcatttg gttgtttgct tcctaaaatt aaggagtaca
     4921 aacctgattc gttaataata gttaaccctc taggcgattc aaaagtaccg ttttggtagt
     4981 tttgccgatc ttctacatcg acgtgtctgt taatatctct actaccattt tggtacccta
     5041 gaactttagc cacatctttt ccaacaaaaa atggttcatc atgtattgtt actgttctta
     5101 cttcatgttg ttcaaagtta aaaatttgcg gtgtgttcat ttttgctcat tcctttcttt
     5161 gatataattt tgaatagaaa gcgaggtgaa aatagtatgg aagaatttaa tatggatgtc
     5221 gacgccttat tcaaacaaac cgtattcaag actgttaata aagaattcaa aatagacttc
     5281 caaaatgacg aatcttttcc aacagaaatt gaattattcg aagaactttc tcagagcgtt
     5341 tctgagtctt tttctcgtca gttgcaaaag aatttttttg atgctcttgt ggatgaattt
     5401 catcaacaac agcactaaa
//
LOCUS       DAFBQW010000118         5171 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFBQW010000118 DAFBQW010000000
VERSION     DAFBQW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.124"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            71..154
                     /locus_tag="LK889_002867"
     CDS             71..154
                     /locus_tag="LK889_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBK5097041.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            279..1016
                     /gene="erm(B)"
                     /locus_tag="LK889_002868"
     CDS             279..1016
                     /gene="erm(B)"
                     /locus_tag="LK889_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK5097042.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1186..1446
                     /locus_tag="LK889_002869"
                     /pseudo
     CDS             1186..1446
                     /locus_tag="LK889_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1609..2274
                     /locus_tag="LK889_002870"
                     /pseudo
     CDS             1609..2274
                     /locus_tag="LK889_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2310..2555
                     /locus_tag="LK889_002871"
     CDS             2310..2555
                     /locus_tag="LK889_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5097043.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2658..3527
                     /locus_tag="LK889_002872"
     CDS             2658..3527
                     /locus_tag="LK889_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK5097044.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3508..4242
                     /locus_tag="LK889_002873"
     CDS             3508..4242
                     /locus_tag="LK889_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5097045.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4275..5171
                     /gene="ant(6)"
                     /locus_tag="LK889_002874"
                     /pseudo
     CDS             4275..5171
                     /gene="ant(6)"
                     /locus_tag="LK889_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
BASE COUNT         1838 a          757 c         1025 g         1551 t
ORIGIN      
        1 ttttgtgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa
      121 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      181 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      241 tatttattta cagatgtaat aacttcagga gtgattacat gaacaaaaat ataaaatatt
      301 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      361 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      421 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      481 cagaaaaatt aaaactgaac attcgtgtca ctttaattca ccaagatatt ctacagtttc
      541 aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac
      601 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      661 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      721 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      781 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      841 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      901 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      961 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1021 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     1081 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggaat
     1141 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     1201 cctaagtgtg gcaacaatat tgtattaaaa aaatcgtttt atggttgttc aaattatcct
     1261 gaatgtaagt ttactttagc tgaacatttt agaaagaaaa aactaaccaa aacgaatgta
     1321 aaagaattac tggagggaaa agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag
     1381 ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat ctctttttca
     1441 aaataaacat ggggctgacc caaaagtagc tcctatatga aaaatagagg tctttccatt
     1501 tagatagtag tggaaagacc tctatttatc ttttgaaagt gaatacaaaa ataaagcaca
     1561 ctctgctata atataggtga ttaatccatt tgagagaggt gctttatcat gtatcaaaat
     1621 tataccataa accaactttg tttgcctata gatcttgaaa ttaaattaga agaaaatgat
     1681 tttgctcatg ccattgtgca gtttgttgac tctattcccg atgaagtctt tcttccttat
     1741 tatcaatcta tgggtagacc ccaatatcat cctcgtatgc tactttccat tattctttgt
     1801 gcctatattc aaggggttta ttcgggccgc caaatacaaa atatgctgat agatagtatt
     1861 cggatgcgct acttatctca agagcaattc cctaactttc gtactattaa tcgttttcgc
     1921 gttcatccga ttatgaacga tattctagat cattcatttg ttcaatttcg tgagctactg
     1981 gttcaaagtg gcttaatcag tggcagtgct ctatacattg atggtacaaa aattgaagcg
     2041 gatgcgaata aatactcttt cgtttggaaa aaaagtattt taaagtataa ggatagcctt
     2101 gatcaaaaag cccttgaaaa ctaccaacaa atggtggaaa caaaaatctt acctgagtta
     2161 atagatgatt taaaggatga actttctata gaagagattg aaaggattcg tcaatctttg
     2221 gaagaaaagg aaaatcaatt aattgaagcg attgaccaaa ccgaaacagt agaagggggc
     2281 tgacccatta ttaataagga gtggacaaca tgattgatgt aaatgagcta ttggaagaag
     2341 ctattagaga aactgaaaat ctaaatgatg gcgaagtttt tcttgttaaa gatttattca
     2401 agggatatgt ggggaatagg atacccagaa aggatcgact tctgcttgga acgttatttt
     2461 taaaccatgt tagcaaaatg aatggtaatt taaaggctat tgaaaagact tcatctaatc
     2521 agcagaggta taaaaagaca attgataagt tttaactatc ttttgtgaaa ttaaaaaaat
     2581 tataaatatg ttgagtagaa gcaaaagatt tggcttggaa tttttacagc agtgaatcga
     2641 taaaaggagc atgcttgatg gtagataata ttattaaatc agtagcagag aaattatcct
     2701 ctctgtctta tatagaaggt attgttttag gtggttcacg tgcaaggggc acccatacag
     2761 aggattcgga tatagatatc ggcatctatt acaattcaga atcatttgac ataaatacta
     2821 ttaatcaatt cgctacaaag ctggatgatg agcatagaaa taaccttgtt gtacctcccg
     2881 gagcatgggg tgattggatt aatggcggcg gatggttagt cataaacggg tatcatgtgg
     2941 atttaatttt acgtgatatt aaacgtgtgg aacaaataat gaaagataca gagcacggaa
     3001 ttgttactgc caattatcag actgggcatc cccatggtta tattagtgca atgtatcgag
     3061 gagaattagc gattagcaaa atactatatg ctaagaatga aagcttatgc gaattaaaaa
     3121 aacaggcaga aacttatccc aatgctttgc agaaaagttt agttaacttt tttatgtttg
     3181 aagcagggtt ctctttaatg tttgtaaaag caaattcggg aacagacgat aaatattata
     3241 ttgcgggtca tgtttttcgt atagtttcat gtttaaatca agtgttattt gcatgtaata
     3301 atgcttattg tatcaacgaa aagaaagcta taaaactgct tgaaactttt gaacataaac
     3361 ctgaaaaata taccgagaag gtaaatcata tttttgaagt actcggtatc tcactttttg
     3421 aatgctacga catgaccgag aagctttata atgaagtgaa tgaaattgta tcggagataa
     3481 ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt
     3541 tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg
     3601 gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc
     3661 ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt
     3721 gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa
     3781 tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta
     3841 agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg
     3901 atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat
     3961 ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat
     4021 tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat
     4081 agaacactga ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt
     4141 gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga
     4201 cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa
     4261 cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag
     4321 aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac
     4381 ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata gaacctagaa
     4441 ccgtttatat ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag
     4501 ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt
     4561 gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta
     4621 aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata
     4681 gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc
     4741 tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt
     4801 ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca
     4861 tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt
     4921 gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc
     4981 tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt
     5041 gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat
     5101 accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc
     5161 gctgatatag a
//
LOCUS       DAFBQW010000119         5116 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFBQW010000119 DAFBQW010000000
VERSION     DAFBQW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.125"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            73..234
                     /locus_tag="LK889_002875"
     CDS             73..234
                     /locus_tag="LK889_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK5097046.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="LK889_002876"
     CDS             253..1530
                     /locus_tag="LK889_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5097047.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="LK889_002877"
     CDS             1540..2196
                     /locus_tag="LK889_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5097048.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="LK889_002878"
     CDS             2196..3572
                     /locus_tag="LK889_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5097049.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3858..>5116)
                     /locus_tag="LK889_002879"
     CDS             complement(3858..>5116)
                     /locus_tag="LK889_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HBK5097050.1"
                     /translation="DIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTHD
                     EAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREKQ
                     FAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADAGYKTPK
                     FVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTRERY
                     REYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRK
                     QTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCS
                     KIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKA
                     RFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1901 a          664 c          900 g         1651 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaattaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaggaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatgact
     4381 cgttgatgat ttttactttt ggtacattgg tttagcagag gacagttcac acattctttt
     4441 ggattggatt tgtattctcg gtacctttct cgagtaaccg ttgaaaaagc gagcatttga
     4501 tcttgcggac aaatataaca atcaaaatac tcgtcataga gaaactcatt ttttgataat
     4561 agcccctttt tccctttggg ccttgaataa ggcaaacaag gtcgaaggtt caaatgagtc
     4621 aaaaagtgaa caaacttagg tgtcttatac ccagcatcag cgacaacaca attgatttca
     4681 ggaaaaagtc tcttcgattg tttgacgagc tgaaaggcga cttgactgtc atgaatattt
     4741 ccaggagtga tgattgtgct aagaataaat ccattgtcgt cgcatgaagt atgggcagaa
     4801 taagcaaatt gtttttctcg ttcgcctttg acgtagtagc cgctttccgg atcagcttta
     4861 cttatttttc tttctttcag cttcacttcc ttttctaagt ccaaagtaaa agggcgtttt
     4921 ccagcctcta ttcgttgacg attgatctca tcttctaatt catcctgata ggctttcgct
     4981 tcatcatggg tcatttcttt cgtaaactta tgtttattgg cgttagcttt gatgtgggta
     5041 gaatccagat acaaattatc ctctgttaca aaacctgctt taactgcttg ttctaaaata
     5101 taagcaaaaa tatcct
//
LOCUS       DAFBQW010000120         4893 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFBQW010000120 DAFBQW010000000
VERSION     DAFBQW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.126"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..563)
                     /locus_tag="LK889_002880"
     CDS             complement(<1..563)
                     /locus_tag="LK889_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBK5097051.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="LK889_002881"
     CDS             complement(750..1400)
                     /locus_tag="LK889_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097052.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LK889_002882"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LK889_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBK5097053.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="LK889_002883"
     CDS             complement(2912..3910)
                     /locus_tag="LK889_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBK5097054.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LK889_002884"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LK889_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK5097055.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
BASE COUNT         1486 a         1051 c          757 g         1599 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tat
//
LOCUS       DAFBQW010000121         4807 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFBQW010000121 DAFBQW010000000
VERSION     DAFBQW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.127"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="LK889_002885"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="LK889_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBK5097056.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="LK889_002886"
     CDS             complement(3250..3768)
                     /locus_tag="LK889_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBK5097057.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1578 a          786 c          731 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagggactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DAFBQW010000122         4753 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFBQW010000122 DAFBQW010000000
VERSION     DAFBQW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.128"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(207..1169)
                     /locus_tag="LK889_002887"
     CDS             complement(207..1169)
                     /locus_tag="LK889_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5097058.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LK889_002888"
     CDS             complement(1171..2610)
                     /locus_tag="LK889_002888"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBK5097059.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LK889_002889"
     CDS             2795..4741
                     /locus_tag="LK889_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBK5097060.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1510 a          817 c          784 g         1642 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cct
//
LOCUS       DAFBQW010000123         4679 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFBQW010000123 DAFBQW010000000
VERSION     DAFBQW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.129"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(91..954)
                     /locus_tag="LK889_002890"
     CDS             complement(91..954)
                     /locus_tag="LK889_002890"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK5097061.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(1096..3669)
                     /gene="mprF"
                     /locus_tag="LK889_002891"
     CDS             complement(1096..3669)
                     /gene="mprF"
                     /locus_tag="LK889_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK5097062.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3810..4616
                     /gene="proC"
                     /locus_tag="LK889_002892"
     CDS             3810..4616
                     /gene="proC"
                     /locus_tag="LK889_002892"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK5097063.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         1462 a         1025 c          809 g         1383 t
ORIGIN      
        1 tcatatctag ccatttcact acaataaaaa actgaagaat accattttag gattcctcag
       61 tttttcatca cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc
      121 ctcacaaaaa ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa
      181 ggcaaaaacg gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat
      241 acgctctttg atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt
      301 tcccattttt ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg
      361 gatgtcttga tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac
      421 actcattctt ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat
      481 ttcttcccct ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt
      541 accgacttcc aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg
      601 gttgatccct cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac
      661 tggaatattc ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag
      721 caataaagca gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt
      781 ttctacaatg attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac
      841 gctcgacatc accatttcca actcatgata cccatcttgc cgtctatata atacatccag
      901 tcctaaattt atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa
      961 agtctaagtc gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta
     1021 gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac
     1081 aagttctagt gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc
     1141 tttttctttt cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt
     1201 cctataagca aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc
     1261 agactggaag gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg
     1321 aaagaaagaa aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc
     1381 cgagtagccc attttctcgt atacaataaa cagattggct aacagatagt cagctaattc
     1441 ctcagattct ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg
     1501 ctgagcggtg atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga
     1561 taatacatat gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag
     1621 ccatttttgt gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca
     1681 agtaaaatgg tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga
     1741 caagtcttct gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc
     1801 caattcatga agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc
     1861 taaacaatct gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc
     1921 aactggatct cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg
     1981 atcttggtcg tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct
     2041 tgtagctgtg cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact
     2101 gccaagcttc gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga
     2161 gatgcccaaa ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg
     2221 atttcctgtt attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac
     2281 gataaacaaa aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa
     2341 ttttgttcga taaaattctt ttctggataa ccacacagct aacagaatca agacatatac
     2401 caatatcaac tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa
     2461 agcaatcgta ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag
     2521 taataaaaag ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa
     2581 taatacttga aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat
     2641 catgatcccc gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa
     2701 cagtcgcgga atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa
     2761 tagaagtccc gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac
     2821 tactgctaca tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa
     2881 agtccccatt ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat
     2941 aggatagatc gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac
     3001 cgcaagggct cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg
     3061 ataaaactcg aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc
     3121 taaaaaacta ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat
     3181 aaaaaagaca tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag
     3241 tgcgactttt gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact
     3301 agcccgcaga cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt
     3361 tacccacgca gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa
     3421 gactctgatc gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag
     3481 aatcatgagc aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca
     3541 actcatgcca ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa
     3601 aaaaatcagt tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt
     3661 atttttcaca ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct
     3721 tatttatttt aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa
     3781 gatatcctaa tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata
     3841 tggcacaagc tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt
     3901 tcgtttccag tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg
     3961 catgcaaaag taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac
     4021 caaaaatcat tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca
     4081 tttctattgc ttcaggaaaa tcattaacag atttagaaac agcgcttgaa gatcctcagg
     4141 cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga
     4201 tttgcaaaaa tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag
     4261 caatcggcag cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg
     4321 gcagctcacc cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga
     4381 atggtttgtc caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc
     4441 aaaaattcct ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg
     4501 gcggtacaac tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca
     4561 tcaaaggaat caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat
     4621 agcaatgttt tttcgtacct tacaaaaaaa gaagagctgt ggcttttgcc gcggctctt
//
LOCUS       DAFBQW010000124         4661 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFBQW010000124 DAFBQW010000000
VERSION     DAFBQW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.130"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(172..474)
                     /locus_tag="LK889_002893"
     CDS             complement(172..474)
                     /locus_tag="LK889_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097064.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(694..1060)
                     /gene="ssrA"
                     /locus_tag="LK889_002894"
     tmRNA           complement(694..1060)
                     /gene="ssrA"
                     /locus_tag="LK889_002894"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1231..2004)
                     /locus_tag="LK889_002895"
     CDS             complement(1231..2004)
                     /locus_tag="LK889_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5097065.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2148..2498
                     /locus_tag="LK889_002896"
     CDS             2148..2498
                     /locus_tag="LK889_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK5097066.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2479..3195
                     /locus_tag="LK889_002897"
     CDS             2479..3195
                     /locus_tag="LK889_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5097067.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3195..4643
                     /locus_tag="LK889_002898"
     CDS             3195..4643
                     /locus_tag="LK889_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5097068.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
BASE COUNT         1550 a          822 c          899 g         1390 t
ORIGIN      
        1 ttataattgg agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat
       61 aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat atccctttta
      121 taagtatggt gataaaatcc gttccgggct acctttttta ggtagcctac tttaatcttt
      181 atacctttct ggatcaacga aagtatactt caaatagtca taacgccgat gatcgctccg
      241 tgcgtctggc acatcagtca cgatatcaaa caaaaaatac acatctttct tcattctggt
      301 ttttgttgcc ggaattttaa agtagttctt attagagtaa tagagattga ttaataagct
      361 atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat ctttgacaaa
      421 tctattcgaa ggatcaaatt taaaccataa ttgtgttttt ccttccatca acataatgtt
      481 cacctcaaaa agagtatacg aactaatgtt ctttttgtaa agtaggaata tcattgataa
      541 tatggatgga acatatatat taaaaagtgt tctttttgaa tgctattttg aatgccattt
      601 tcattcattt aacgaaaatc tgcgaaaata aaaaatgctc aaacccttat aaaataagga
      661 tttaagcatc tagcgaaatc tgctgaaatc tcacaatgga gacggcggga gtcgaacccg
      721 cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt aaggattcac
      781 tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct ctttttcaac
      841 ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc gagcacatgg
      901 gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa gagttgtttt
      961 cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct acgaaacgca
     1021 attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac aagggttacg
     1081 tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata aaaaagcgaa
     1141 tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa gcgattttct
     1201 taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg
     1261 gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt ttaatccgtc
     1321 ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg
     1381 accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt gcagacgttt
     1441 gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag gaccattccg
     1501 ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt gagataagat
     1561 cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga
     1621 atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg
     1681 atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca
     1741 ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc
     1801 attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc
     1861 tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt
     1921 tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa
     1981 agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct
     2041 taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag
     2101 tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca
     2161 gtattggtat cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa
     2221 atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc
     2281 tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg
     2341 gctattattt aaagacatct gggcatacga attattttct gattgaaatg cttctgctag
     2401 tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg
     2461 aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa
     2521 atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa
     2581 gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac actagacctg
     2641 aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact
     2701 gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag
     2761 atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg
     2821 aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagt agtttcagat
     2881 gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat
     2941 ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta
     3001 gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat
     3061 cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc
     3121 gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat
     3181 tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttctgc ggaatggtcg
     3241 cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag
     3301 aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct
     3361 atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga
     3421 cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg
     3481 tcatttatcc gactaataca gctttactga attttacctt aacagctgat caatggaaaa
     3541 atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac
     3601 aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg
     3661 tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt
     3721 tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat
     3781 tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt
     3841 ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca
     3901 ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc
     3961 gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg
     4021 gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta
     4081 aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg
     4141 aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat
     4201 tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat
     4261 tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg
     4321 tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag
     4381 aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac
     4441 aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa
     4501 aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag
     4561 gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg
     4621 acgtagaaat caaaaaagag taaaaaacaa gcggtataaa a
//
LOCUS       DAFBQW010000125         4617 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFBQW010000125 DAFBQW010000000
VERSION     DAFBQW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.131"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(16..963)
                     /locus_tag="LK889_002899"
     CDS             complement(16..963)
                     /locus_tag="LK889_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK5097069.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1141..1944)
                     /locus_tag="LK889_002900"
     CDS             complement(1141..1944)
                     /locus_tag="LK889_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBK5097070.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2090..3004
                     /locus_tag="LK889_002901"
     CDS             2090..3004
                     /locus_tag="LK889_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK5097071.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3052..3522
                     /locus_tag="LK889_002902"
     CDS             3052..3522
                     /locus_tag="LK889_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBK5097072.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3607..4215
                     /locus_tag="LK889_002903"
     CDS             3607..4215
                     /locus_tag="LK889_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBK5097073.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4349..4549
                     /locus_tag="LK889_002904"
     CDS             4349..4549
                     /locus_tag="LK889_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5097074.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1478 t
ORIGIN      
        1 tattgttttc agctattaat atcctgcaga caattttact tgattggttt gaagctcatg
       61 ggtggcaaag tagcttttca gattggttaa aaaaatagcc attaatttct tttgaaaatc
      121 aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg tccattttcc aaagaggatg
      181 atcttgtgga agaggctctt cctcaaaaac gtctaatgct gcaaaggaaa gttgctggtt
      241 atttaaagcc tgcaccagat cttgtgtctt aacagaagca ccacggccaa cattgatgaa
      301 tacagatttt ggatcaaagt gttcaaacat atgctgatca aagagcccag ttgtttcctc
      361 cgttagaggt aagatattga ctacgaaatc tacttcagga agaacttcct ttagttgatt
      421 taaagaaaaa gtcttttcca ttccttctgc tggatgaccg gatgtattga ttccatagca
      481 gttcatgtct aatgctttgc atttttttgc aatcattttt ccaatatggc cagttccaat
      541 gattagtatt tttttccctt ggatagattg gtaatggacc gatgaaccta accatttcat
      601 gtcactctgt gccttttgtg cttgaaaaag accacgagaa tacccaagga tgattcctaa
      661 gacatgctcc gtaatcgatt cactgtggat gccactacca tttgaaagta aaatatgttt
      721 ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct gagatagatt ggacccattt
      781 caaggtagaa gaggctaata gtttttcttg gtagtctgag ttccagccca aactgatctc
      841 tacattttgt agatcctctg gttgtaattc tgttttaaag atataaccag gcgctatagt
      901 atggatttct tcgataaatt catcacgaaa tggacgatct gcataaatga ttctttctga
      961 catagttaat ttcccgcctt tctttactct aagtgtagcg catacaagaa gaactttgaa
     1021 gcgccttgct tgttttttga gaaagaaata tttttcaaat aaaaaagggg ctgtgacaaa
     1081 aaaatgtcac aacctctaaa agggtctatt gaagtagaaa taacgagttg tctttatttc
     1141 ttaattgatc gatatttttg cccatccttt gacagcttga gcaaaaataa agattcctgc
     1201 aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag accatcaaga ttgttgcaat
     1261 tatagtaggc aatgaaattg aatagatcaa tgtttttaaa caatcaagaa aagtagcttt
     1321 tgttagtcga aagcgtgcat agagaaaagc agcaatgttg gtaaatagca aagtgatcag
     1381 cagattcaga aaactaggat aaatagaaaa tataaatgtc aggactttta tccaaagtgg
     1441 tatagatgct tcacttaaaa aagaacggag ctgcttacct gttaagtttt ttaaagattc
     1501 attggtgtag ggaagttcta attggttatt gttcaacaaa gcagaagtcg tgcctgtatt
     1561 aggaagagct atgataagtt tgttttttaa catcccgaca ctaacaaaat tccccatcaa
     1621 atcagtagag atgtcctgtt ctgttcgttt tccctctgga tcaaatgtga agataatgga
     1681 gtttgtttgg taaatgaatc cttctttttc ttcggtttga agctgcccgt tttggattgt
     1741 aaaatcagga atgcgagtag caatcttttg cccatccaat tgaatgttgt ttaagacttg
     1801 aaaaacttga aaagagattg gcaaagccat aattatactc aaaactaaaa gatagacaat
     1861 ggctttccaa aatgggacat ttctcgcctc ctttaggtct ttgaatcgta taaaggaact
     1921 gataattaat tgttttgttg tcataagtac tcctcatcta gtagtgtcat cacttgcaat
     1981 tctagcgaag tatcaagtga aatacaagtg ctttaaggaa acagctgaat gattggtgaa
     2041 atatcaaaaa aatgttattc ttgtctaaag actatccaat ataaataaaa tggaggaagg
     2101 aatagtgcct acacagccac agagatcatc aaaaaaacta tttggagtag tggttttgaa
     2161 tgcggtgatc actttatcag aatttattgg agggattatg tcagggagtt tgtctcttgt
     2221 ttcagatgct ttccataacc ttagtgatac attggcaatt attttttctt acggggcaca
     2281 aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat ggctaccagc gtttagaaat
     2341 attgagtgct ttcatcaact cattcatatt gatcatatta tccttgtttt taaccgttga
     2401 agcattcaag cggttcactt caccagaaaa gataaatagt catttgatgc taacagtagc
     2461 agttattgga ttgctcgcga atttgttctc agtattatta ttacgacaag aagcagatga
     2521 aagtttgaat ataaaatcta gttatttgca tttgttaagt gatacactgt cttcggtaag
     2581 tgtgattatc ggagcaattt tgatccgttt tttcgatatc tactggatcg atccagttat
     2641 tacactgatc atttcgatat atatattgat agaagcgata atggtgatca aaaaagctgc
     2701 cgctattttg atacaaagtg cacctgctat cgactatgaa aaaatggaac aagaaattga
     2761 agcaatcgat ggggtaaagg atgttcatca tgtccatatt tggcagtata gcgaaaagat
     2821 aattattttc gacggacata ttgattttga agatcagcta ctaagcgaga ttgaaaagat
     2881 atatcctcgt ataatttctt tattaaaatt aaaatatggt attacacatg tgacgatcca
     2941 agcagagaca aaggcaaagg atcaaaaaaa attaattttt ttaaataaag atgaaagtta
     3001 ttgaaatata gagtatgata aataagaatt tttaataagg taggttggct catgaagcag
     3061 tatcgacagt taaaaggata tttagaagca aaaggatatt gggagatact agtctatctc
     3121 ttttttggtg gtttggctac gattgtaaat tttgttagtt ttgctctcgc acgccaatat
     3181 tttgatttaa gtatggcctt atccaattct atttcgtggt tttgttctgt attgtttgct
     3241 tttgtaacga acaaactttg ggtattccat tcaaaatcac cgaattttac acatgcactt
     3301 attgaatgcg gtaagttctt cttctaccgt atcctttctt acggattgga catgggggca
     3361 atggtattgt tgatcaatgt tatgaacagc aatgagtatg tggcgaaaat catcactcaa
     3421 attattgtta tccttgcgaa ctatattttc agcaagttgt ttatttttaa agaaactgag
     3481 gtttttgaag aagaagtagg tcaaagtgaa aaagacgact aggcataaca attgaagaat
     3541 ttccttctct ttgttactct ttgtaatgta gacattatta agaaaacatt gaggaggaat
     3601 ttttttatga cttacacatt accagatcta ccttatgcat atgacgcatt ggaaccttat
     3661 attgacgaag aaacgatgca tctgcatcat gataagcatc acaatactta tgtgacgaat
     3721 ttaaattcag caattgagaa atacccagaa ttaggcgaaa aaacaataga agaattatta
     3781 tctgatatgg acgctattcc aacagatatc aagacagctg tacgtaacaa tggtggcgga
     3841 catgctaacc attcattttt ctgggaaatc atggcaccaa atgctggtgg cgaacctaca
     3901 ggagaaataa aagaagcgat taatgaagct tttggtgatt tttcttcttt taaagaagaa
     3961 ttcaaaaaag cagccgctgg acgatttggt tctggatggg cttggcttgt aatggaaaat
     4021 ggaaaattag ctattacctc tactgcaaat caagattctc cattgatgga aggaaagaca
     4081 ccaattctag gtttggacgt ttgggaacat gcttattatt tgaaatataa aaatgtccga
     4141 ccagattata ttgcagcatt ctggaatgtt attaattggg atgaagtaaa caaacgtttc
     4201 gaagcagcaa aataaccatt tcctccgtca tatcagtctt aaaaataatt tggaaaataa
     4261 aagagcgaaa gcttgctagc gttttctaaa ttgggtataa tgttcttgca aggttacttg
     4321 caagaacttt tttggaggat atacatttat ggaacacgga acggttaaat ggttcaacaa
     4381 tgaaaaagga tttgggttta ttacagtaga cggcggagat gacgtatttg ttcatttctc
     4441 agcaattcaa ggagacggct tcaagagcct agaagaaggt caagaagtag aattcacgat
     4501 tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact aaattataat aatttgttga
     4561 ataaagacac gacttcttag agagtcgtgt ctttttgtct atttatgaag tttttag
//
LOCUS       DAFBQW010000126         4522 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFBQW010000126 DAFBQW010000000
VERSION     DAFBQW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.132"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(559..1599)
                     /locus_tag="LK889_002905"
     CDS             complement(559..1599)
                     /locus_tag="LK889_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5097075.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="LK889_002906"
     CDS             complement(1948..2274)
                     /locus_tag="LK889_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097076.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3064)
                     /locus_tag="LK889_002907"
     CDS             complement(2261..3064)
                     /locus_tag="LK889_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5097077.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="LK889_002908"
     CDS             complement(3417..3707)
                     /locus_tag="LK889_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBK5097078.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="LK889_002909"
     CDS             complement(3843..4022)
                     /locus_tag="LK889_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097079.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="LK889_002910"
     CDS             complement(4019..4369)
                     /locus_tag="LK889_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097080.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..>4522)
                     /locus_tag="LK889_002911"
     CDS             complement(4362..>4522)
                     /locus_tag="LK889_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HBK5097081.1"
                     /translation="EENLDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAG
                     GNGGIKND"
BASE COUNT         1372 a          785 c          636 g         1729 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gt
//
LOCUS       DAFBQW010000127         4479 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFBQW010000127 DAFBQW010000000
VERSION     DAFBQW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.133"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(367..1158)
                     /gene="msrA"
                     /locus_tag="LK889_002912"
     CDS             complement(367..1158)
                     /gene="msrA"
                     /locus_tag="LK889_002912"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5097082.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1349..1984)
                     /locus_tag="LK889_002913"
     CDS             complement(1349..1984)
                     /locus_tag="LK889_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK5097083.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2250..3302)
                     /locus_tag="LK889_002914"
     CDS             complement(2250..3302)
                     /locus_tag="LK889_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5097084.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3315..4253)
                     /locus_tag="LK889_002915"
     CDS             complement(3315..4253)
                     /locus_tag="LK889_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK5097085.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1459 a          951 c          689 g         1380 t
ORIGIN      
        1 tatagttatt gtttgcgctt ttatacggaa tttagttcat cattaatgtg gaattcgatt
       61 aggtgtctat taattatcaa gatgattctc tacaaaattc catagaaaaa tcaagattct
      121 acaattcgaa ttctctttca catggaaata aaggctagta aaaaaacagg agttgtgaca
      181 aaagacttgt catacctcct gtttccttgc gacttgaagc tgaatgcttt gcgccacaac
      241 cttttcctcg gtgttctcaa agcaactcct cgaaataatt cataactgtc caaaaatatg
      301 aaaagctatt tttgtcagtt gctcattatt ttgtcggagc tgaacgcttt gcgccacaac
      361 cttcctctat ctatgtcgct gaaaaaataa atgaagctga tgcgaatgct tgatcttttt
      421 atatctttta ggttgcttct gataaaattg ttgatgatga ttttcagctg gccaaaaagt
      481 ctttgcttgg cgaatctctg taacgatagg acggctaaat cggttagatt gaatcagttg
      541 ttctcttgat ttttcagcca attgcctttg cttttcatta acaacaaata gaattgaacg
      601 atattgtttc ccccgatctt gaaattgacc aaaggcatct gtagggtctg aaatttgcca
      661 ataaatctcc aacagctctt cataggttat ttgacgtgta tcaaatatga tctcaacagc
      721 ttcaacatgc ccagtatatc caccactgac ttgatcgtaa tttggttttt tggtctctcc
      781 acctgtataa ccagataaca ctgagattac acctggtttt gtttcaaaag gctcaaccat
      841 acaccagaaa cagccaccgg caaaaatcgc ccgttcttgg tataagggtg aagttatctt
      901 atgtttggca taatcataag cctttgcact cgctgactcc cctgtgattc ttaaataaaa
      961 atccatcgca tctggtgtca aattatctcg tagtgctagc ggacgcaagt tgacctctag
     1021 tttttctatc acctgcttta tcgacatttt tgctaggtct tcttttgcct cttcaaattg
     1081 tgttctttcc cattctcttg tatttcgatt taaaataata ttataaaggt ctatttccat
     1141 ctgctccaca tctaacatga agtcaccctc tttcaagttt tcttcttatt cattcaaaaa
     1201 atgactacct gaaatttagc acaacgcata acaagttaag actaacttgc ttttcgctct
     1261 cctcatctct attcttagtc gaacacatcc acacgaatgg ttacccatag agaaattctt
     1321 ttacttaatc agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa
     1381 tcatcggcaa atagttaaag aaaaaactta agcgttgaaa gaagcccaat tgcttaaaga
     1441 aggcaaactg actaatcgac ggaatccgtg ctaatctgta aagacctgca attagtaaac
     1501 taataactgt taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa
     1561 gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat
     1621 tatggatcca agtcgataga ttccaagagc tttcattcgt attgatactg aataacccag
     1681 taaaaataca atcacctatc ccatataaac caactgttgc aacagcaagt attgcaagtg
     1741 tttttgatgt tccaataaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc
     1801 cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca
     1861 cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg
     1921 tcgataaatc actaatcaca cctaataata aaaaataaaa accatatttt tgcaaaaaaa
     1981 tcattttctt ctcctacttt ttagtgatta ttcgatacat ccaacaatca aattttttct
     2041 cattttaaca catattttta ttctagttgc cttttgaaca atcaataaca taaaggtgag
     2101 tttcttctac attttaaaaa atcgaatggc actgaagagt tcagttgtca gcatcaatct
     2161 cctactcaaa aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc
     2221 attgattgaa aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga
     2281 tcatacagtt taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg
     2341 atgactaacc aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac
     2401 ggaccaacaa gtccgaccac gccgaaacca gcagaagcag gcgtacctga aatagaaaat
     2461 agagcgacag gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt
     2521 tttctaaata gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca
     2581 cctgacttat taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc
     2641 atcgcagccg caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt
     2701 gaaataggag agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc
     2761 ggttgtaagg tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca
     2821 aaagggaata aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac
     2881 acaatcgaca ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat
     2941 gctgtcagca tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca
     3001 cccaaatcag gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc
     3061 atcattctca tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt
     3121 gttgctagtt gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta
     3181 aaatatttta aaatggcacc taatactgca ttaggaatca atccaataat gattcctgtt
     3241 gctgtaccag cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc
     3301 attcaatctc tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc
     3361 aagaaatcac aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca
     3421 ttgatataat cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga
     3481 taatgtaacc caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg
     3541 acatctagtt caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc
     3601 accattttat gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg
     3661 catagtccgt tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg
     3721 tcagaaaaat gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct
     3781 gcttcttttc catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc
     3841 tgaccaggac cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt
     3901 ggtacaaatt tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct
     3961 gtatcttttt tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg
     4021 gtaaatagaa taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt
     4081 ggtagttttg cttctacctc tttaccatta aaatttgctt gtaatccatg ctctttgatt
     4141 tgttggacat gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc
     4201 attaacccaa agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct
     4261 tcctcctttt tctttctatt atattcattt gagaatcatt tcacaacctt gttttgttat
     4321 tcttctctta tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata
     4381 aaaactggaa tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata
     4441 gaaacattca tagaaaaaac ctcactcctc tgattataa
//
LOCUS       DAFBQW010000128         4465 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFBQW010000128 DAFBQW010000000
VERSION     DAFBQW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.134"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            574..1008
                     /locus_tag="LK889_002916"
     CDS             574..1008
                     /locus_tag="LK889_002916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097086.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="LK889_002917"
     CDS             2298..3221
                     /locus_tag="LK889_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK5097087.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="LK889_002918"
     CDS             3237..3776
                     /locus_tag="LK889_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK5097088.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DAFBQW010000129         4433 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFBQW010000129 DAFBQW010000000
VERSION     DAFBQW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.135"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(543..2048)
                     /locus_tag="LK889_002919"
     CDS             complement(543..2048)
                     /locus_tag="LK889_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5097089.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDSMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVLEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LK889_002920"
     CDS             complement(2045..2731)
                     /locus_tag="LK889_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5097090.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LK889_002921"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LK889_002921"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK5097091.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          728 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct ctagaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat actgtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAFBQW010000130         4312 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFBQW010000130 DAFBQW010000000
VERSION     DAFBQW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.136"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(75..1073)
                     /locus_tag="LK889_002922"
     CDS             complement(75..1073)
                     /locus_tag="LK889_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK5097092.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="LK889_002923"
     CDS             complement(1148..1444)
                     /locus_tag="LK889_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5097093.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="LK889_002924"
     CDS             complement(1437..1730)
                     /locus_tag="LK889_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097094.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="LK889_002925"
     CDS             complement(1876..2427)
                     /locus_tag="LK889_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBK5097095.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="LK889_002926"
     CDS             complement(2601..2900)
                     /locus_tag="LK889_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBK5097096.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3235)
                     /locus_tag="LK889_002927"
     CDS             complement(2927..3235)
                     /locus_tag="LK889_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK5097097.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
     gene            3540..4223
                     /locus_tag="LK889_002928"
     CDS             3540..4223
                     /locus_tag="LK889_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097098.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT         1278 a          815 c          634 g         1585 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttt aggaagtgta cctatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaagttgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttgtcc tttattttga aacatttctt
     3241 ttccctcctt tgctataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcga aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4261 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aa
//
LOCUS       DAFBQW010000131         4306 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFBQW010000131 DAFBQW010000000
VERSION     DAFBQW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.137"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            149..1138
                     /locus_tag="LK889_002929"
     CDS             149..1138
                     /locus_tag="LK889_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBK5097099.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPRMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAERLNDEIDLFNHALSKMFRRKKVQIAIKGYVRKLEITYNKEREDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGVNPDGTDEITQLDVRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1135..1554
                     /locus_tag="LK889_002930"
     CDS             1135..1554
                     /locus_tag="LK889_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097100.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1533..1871
                     /locus_tag="LK889_002931"
     CDS             1533..1871
                     /locus_tag="LK889_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097101.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQI
                     SVINSKEKGM"
     gene            1875..2237
                     /locus_tag="LK889_002932"
     CDS             1875..2237
                     /locus_tag="LK889_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097102.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2459..3865
                     /locus_tag="LK889_002933"
     CDS             2459..3865
                     /locus_tag="LK889_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBK5097103.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1501 a          602 c          996 g         1207 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt gatttatagt tttaattgtc taagtcaaaa
       61 ctaaactaga aaggagagca atcttaattt ttgttatcgg cttttcagcc ttttttgtat
      121 acaaaatttg tagattcagt atagcaagat gaaaaagaaa aagcaagatt tgattaacgg
      181 aacatttcga gaaaaaaaga aaaatttgaa aaaagtggtg gcatttagtg ataatcgaat
      241 ttctcccaga atgcgtgaat tgatgacagg ctgtggttca tatttagaat ttattgcaac
      301 gattgataag gagaaaaaga aattggtcca agctcacttt tgtaaaaatc gtttctgtcc
      361 gttatgtgct tggcgtaaag ctcgtaagga ttcgatgatg ttgtcgataa tgatgcaagc
      421 cattgcggaa gaaaagcagt atcaatttat ttttatgacg ttgacgacac caaatgtgaa
      481 agcggaacgt ttgaacgatg aaattgattt atttaatcac gcattatcga aaatgtttcg
      541 tcgaaaaaaa gtgcaaatag cgattaaagg ctatgtgcga aaattggaaa tcacatataa
      601 caaagaacga gaagactata atccgcattt tcacttgatt ttggcagtaa ataaatcata
      661 tttcacggat aaggattatt atatcaagca atcggaatgg ttagatttgt ggcgggaagt
      721 gactgggaaa acaggggtca atcctgatgg tacagacgaa attacgcagt tagatgtccg
      781 taaggtcaaa ggattccaac aagaaaaagc agtacaggaa gttgcgaaat attcggcaaa
      841 agattttgaa ctgaccgaaa gtcaagaagt gtttgacaca ttctatcatg caatgaaagg
      901 tcgtcagttg attacgttta acggtgtatt caaagaatac aagaaaaaat atgagtcggg
      961 tgaccttgat cgttataaga aaaaagatga gaatgaatat ttttggtttt tgactgcttc
     1021 gtggatgagg aaagaatcga aatatgcggt tgattatcgt gagatgagtg ctgatgaaag
     1081 aaattatttt tcaggaatgt atgtcgatga aaaatctgtt gagggggaaa atgaatgact
     1141 gattcaacgg ataaaatgac aattaaggcg cttgctgatg aactaggaat aaccaaaaat
     1201 acagtgagaa attatctagt caaagccgga tatgatcttg ctcggtacaa gaaaaatgga
     1261 attatcttgc tagacaaaga gttgattggt gttgctcggt caatttatcg gtcaaaaggt
     1321 ggtcaattga ctggtcattt accggtcaat agcacgggca gtgattttcc tttggaaaaa
     1381 gagaatgagt ttttgcgtga tcaattagct gagaaggaca aacatatttc tgaattaaca
     1441 gaaatgattc gtgatcaaca aaaattgacc ctgcttcagc aacaaaaaaa cgatcagttg
     1501 ttactagaaa atcaagaatt aaaaaatcga aaatggtggc aattctggaa gtgagatgtg
     1561 acatggataa aattgatttg agagaagaaa cagtcaatag aattgaagtg atgtatgaac
     1621 ggttagctga ttatttgaat ctttcggatg aagataatta tagtgagatg cttgaacgaa
     1681 tgatggatgt tctcaactcg gttatcaatg ctgatctagc aaaatcttca gttttgacta
     1741 atgaatttgt tcataagcat ttagcgcaaa caactgatga agctgaaaaa gaattttggt
     1801 tgaaattaat gagtcatatt atgcagttaa accaaatttc cgttattaat tccaaagaaa
     1861 aggggatgta gtttatggga atgactttag cagatattaa agtgcaagaa gaaaaattaa
     1921 aaaaagaaca ggcgaaactt cgccaacaga agaaacgatt gattgagaaa gaaaatgctg
     1981 caatgggcaa ggctcttcgc aagcgttttg ggtttgaatc ttttgctgat tttgagcggt
     2041 ggctatcgtc gatttctgac gatcagcaac acgaaaaatc agtgggtaat gagttgcgag
     2101 ctgatgaaaa atcagcacca agcgaacgga attaccctaa cgaatctgac ggaatattcg
     2161 gagggatacc gaatagaaaa agtcagatga gtgagagtgg atttgcgtca atgtttagtg
     2221 acgtaaattc acgataagcg aatattttgc atgaagcaaa ataaagcgta aaaggagttg
     2281 agagcgtgac acgctttcgg ctcctttttt cgttgtcact tgtgacggcg aaaaccctcg
     2341 gagagcgttt ggaattgtga cagtcatgtc acaattagga aaacgttact ctttgacaaa
     2401 gagtaacaga aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggtttaactt
     2461 ggggtttagt atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg
     2521 tgagttaact gatagccaaa aaatcaatga ctggcataag catattgatt tcacaaaatc
     2581 ggatgaaaat atctatttgg aacaaataaa tattcgtgaa aaatatgaag aattattcgg
     2641 tgaagccgta gaaaaatata atgcaaagca gaaaagagcc gatagaaaaa ttgaggacta
     2701 tttagcaaag gttcgcaagg ataaaaaaat ggaaccacag cgagaattta tcgtgcaaat
     2761 tggtactctt gatgattttc gtactactcg tgatgatggt tcttctactg gcatatcgga
     2821 acaacaagca gaacaaaatc gagtgattgc caacaaaatt ttagttgagt atttcaaaga
     2881 atttaaagaa cggaatccta gtttatccgt ctacaatgct gttattcata atgacgaagt
     2941 aagtccgcac ctgcatttga atattgttcc tgttgctgaa gggtataaac ggggtgttca
     3001 aaagcaacca agttttaaca aagccttgca acagcagggt ttacgagcag ataaagagaa
     3061 tggtcgagtg atttggaata attttaggaa tcaggaagtt gagtcaattg aacgtttgat
     3121 ggcagatttt ggttgggagc gtgagttagt cggtactaac aaaatcaaag atattcatga
     3181 atacaaagaa atcatgagcg aaatttccga attgcgtcaa acggccgtaa aagagcgtga
     3241 gagcgtttct gatgagttga ggtatatttg taggcaaaga gaagaaaacg agcgtgagaa
     3301 ggctctggtg gcttctgaga gagcgaagat tgaagaagag aagaaaaata tgctgaatgt
     3361 aaaaaaagag atggcggtca ttttccaaaa agatatggtt cctttagaaa gtttcaagag
     3421 gcttcctgat ggttcgtatc atttatctgg gaatgatttt gttgatttgt atcaacgagc
     3481 tacgagagca acaaatgcca agagaaatgc tgataacatg agtgagcaat atttacagat
     3541 gcttgagcaa aatacagaat taaatgagta tgtagctgca ttggaagaag agcgtcattt
     3601 gtctggtaga aaacaattag cagatttaca agtggaaaat aaaaagctga aagaagaaaa
     3661 tcgctcttta cgccagcgtt tagagcgatt aaaaaacgca tttcaaaaat cgatcactcg
     3721 tttgtctatc cgttttgggc ttgcagaaaa ggatatgggg ctttctgagg aattagaagt
     3781 attagagcag gagaaaacct tattttatcg tcaaaacgtc tcacagcata aattagagcg
     3841 ttctgaagat gagatcgagt ggtagttacg atgtgcagtc aacggtaccg gaggcggttt
     3901 gtgccgagga taaggagttg acggctaaac cactagccta ttaagctgac atttgctaaa
     3961 aaatggcaag tccatttttt ggccaactta gcgttagcga agtttggggg cgcccttttt
     4021 aaagggtgcg gttttatgtt tgcatgacta agaatgagtg ggaaaattta acagccctat
     4081 ctaacaggcg gttaaatgta caaaatatgt acaccccctt aaaaatgcag tggtcgattg
     4141 atcgaatttt atgtctttgg gttttggggt tttaaaacga aaaaaagggg gcgattttcg
     4201 ccctcttttt tgatgttttt tcttatcttg atactatagg caactatttt cggcagaaaa
     4261 tcggggctat agtatgggtc tatcccttgt ggctctaagg aagaac
//
LOCUS       DAFBQW010000132         4115 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFBQW010000132 DAFBQW010000000
VERSION     DAFBQW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.138"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            97..711
                     /locus_tag="LK889_002934"
     CDS             97..711
                     /locus_tag="LK889_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5097104.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(876..1880)
                     /locus_tag="LK889_002935"
     CDS             complement(876..1880)
                     /locus_tag="LK889_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097105.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(2339..2596)
                     /locus_tag="LK889_002936"
     CDS             complement(2339..2596)
                     /locus_tag="LK889_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK5097106.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2589..2858)
                     /locus_tag="LK889_002937"
     CDS             complement(2589..2858)
                     /locus_tag="LK889_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5097107.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(2989..3168)
                     /locus_tag="LK889_002938"
     CDS             complement(2989..3168)
                     /locus_tag="LK889_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097108.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(3549..>4115)
                     /locus_tag="LK889_002939"
     CDS             complement(3549..>4115)
                     /locus_tag="LK889_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097109.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         1287 a          763 c          587 g         1478 t
ORIGIN      
        1 taccctaaat agacactggt ataacattga aaaatatggc ttcctcgtgt atgattaggg
       61 tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata catacgtgtc
      121 tcatcgatcg atcaaaatga acaacgccag attgaagaaa tgaaaaaatt cggtgcagag
      181 agaattttca tcgagaaaca atctggcgca acgattactc atcgacctgt tttccaagaa
      241 gcattagatt acgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga
      301 agaaattatg acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgaagctg
      361 atcattacta gtttaccgat catggctgaa gcaattggca atcctctcct ggataaattc
      421 attaaggatc tgatcatcca aattttggcc atgatcgctg aacaagaacg gactgaatcg
      481 aaacgtcgcc aggcacaagg aattaagatc gcaaaagcaa acggcgtcta caaagggcgg
      541 cccaaattat acagtgcaga cgcaaaagat ccccaacgtc gccttgttta caaaagcatc
      601 gttgaagatc ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta caatgttacg
      661 agacaaaccg tgtatagaat aaaaaaagac agcatggtca atgacaaata gttgatcatg
      721 ctgcttttat tatcttctca tctttaattt cctacatata attactatct ataaaattaa
      781 gtcttactca gaacagataa agcataaaat tccaacaaac cattttttac ttaactaaat
      841 aaagagtctg ttaatataat cttcacttat ttacgttaat cccctactgt atttgttggt
      901 tccttatcta tattttctac aaaatcctta ttatcttgtg atttagcttc gtataattct
      961 ttgtatgtcg acacattggt tatactaatt cctatagcta gttcttgaga ctctactttt
     1021 ctctctagct cctttgacaa agtgatttga gatttatttg gttccgttgg agtcaattct
     1081 atagattctt cataaaaatc tctatacata aagctatcat taattttata aaaaaaatta
     1141 aatgttttta gcactgccat ataacattca aaacattctt ctatagttgg tttacttcct
     1201 tgaggagaat gaaccaattt attacgtttt tctatgaaat ttttaatttc ctttcttatt
     1261 tctttcggaa actcaatatt aaagtaccct ttaaacatgc tccctaataa tttgtctaca
     1321 gggggagatt gtaaattctc aaatattaga gttaaatcag gtttctcttg ttgataaaat
     1381 tcctttaccc ctatttcaca ggctgtaatt atcgaaataa acctatctct agtatttgat
     1441 atatttttac ttcttttaag gagtataaga gaaaagggga tactttcgtg tccatgcatg
     1501 taattaacta attgttgagc ttcagaagtt atatctctaa ttatatagtt aacggaatta
     1561 ggaagtgtct ttaactctaa tgaaatttct atccaattac tatcttctaa tctgtatatg
     1621 ctcgaagtat aatttgattc gtcagcaagc atcgctacat cccaaacact gatcataaaa
     1681 tcttttaaac gttcaatcat ttcagcaata ttgctaaaat cagctaattt ttctttagaa
     1741 taaaaaaaaa catttatatc agaatcaata aattctattt tttttattac tatatcgtca
     1801 tcattatctt gaccatttat gtttattgtt ccctgagtgg ggagtacttc tttcaaattc
     1861 aagttaaatt tatattccat tacttctcct ccaatatata aaaattactt aactgtatcc
     1921 aaatttaata tgtaaataaa tcattaattt ttagctaaat tctatgagaa gtttattttt
     1981 gttgtttttg ctaattaact aaaactagta caaaaaattt aaaatagatt attaaaccta
     2041 ttgcttattc tattcagtca tatatataag cgtcatcctt tagtaactaa catcccacac
     2101 tacaatagta ttataacata tctattcaac ttcaaattgt tgaaatttaa gccatttata
     2161 tttacttttc ctcaacttta gaaatgattt tactaacctt atatccgtaa tacgcgtaac
     2221 aaacacattc attaataaca atcaacacta agctatatta gctttttaaa tgactagggt
     2281 aaataaaata aggatcaaaa acttttaagt ttctgaccct ttccttactt ccgattggtt
     2341 aatagtgatc ttttgcagaa taaataaata tcgtttcatt ttcaactcta tatatcagtc
     2401 tatgttcatc tgtaattctt ctggaccatt ttccagataa atcatgcttt aatggctcag
     2461 gttttcctaa tccagcaaag ggggaacgat cgatatcttt tattaacttg ttaatctttt
     2521 ttatattgct tttgtttcct tgctcatgcc aataaagata atcatcccaa gcatcatcag
     2581 accaagcctt aatcatcaga ttcaacctcg attaagtcat gtgttttaaa tgcacctttg
     2641 gagaattgtt catctcctcg acgaattttt tccatgacgt aattattaga aagtgttctc
     2701 aacgtttctt gcatagaatc ataatctctt tttgataata caacaactgt atcttctaca
     2761 tctttacttg ttacaataag tgtttcagca tcctcattaa cttgtttcat ataactacgt
     2821 aaattttggc ggaaatttga ataagctact gcttccattc ctttcacccc ttatttattt
     2881 ctataaaaca attgtacatt atattgtaca attaagcaaa tgattaatat tctaacttct
     2941 tataattaaa agtgctatca cttttagttt ttaggataaa aagtgacgtc actttttgag
     3001 tgctggtacc tcaaatcctg ctttttcttg ggcaatcaat aaatctaacg caatacacat
     3061 aatcctggat tcacttttgc cgtacttctg ctttagttgc tgcaattctt tttgtctctt
     3121 ttcatccaac gtaacgggta accgtttatt tcctattgca attggcatta gtactctctc
     3181 ctttcatcga aataacccta tgaaaaatat ggctgcaatc atcttgaata gtagactcac
     3241 aagggctaat acaaagcgcc aaaagatcaa acaaaatacc agtattgcca aaaatactac
     3301 gaagtttaaa tgcacagctg cagcatggcg cataagtatt tcgccaacaa ataggactaa
     3361 caatagagga atgattttat tcatatggat ctccttcttt ctcttaggtt atcgggtgtt
     3421 gcccgcttat ttcgcaccac ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa
     3481 gaaccattcc cttagttact aggttttttg tccttatttc acacattctc acttttcggt
     3541 ataaatgatt attgcctttt tttcctttca aatcgttgat atggcattcg tttacggtca
     3601 agttcatttt ggatttcttc tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa
     3661 gtcgtgtacc agtcgtcgcc tgggttatct acttcttcta catattccct caaaaatgct
     3721 tcgggtacag cgagttcata atccattccc attaacacga tagattccgt tccacacgtc
     3781 aaaaatctag taaatacaag gtaataatcg tctatcgtcg catctttgct gtttagctct
     3841 tccatttctt cttccattaa ttccatgctc tcaaaaattg aataatattc tgattctgag
     3901 taatagtgtt tcctgcaaat cgtgtagctg acaaagtttt cgaccagctc actcctttct
     3961 aggtaaacat tcatgatgta tttttctgtt tctagtcgta gaagctccgt tccttttttt
     4021 accgtttcag gacgacgttt atacacgtct aaacgattat ggctgactgt gaccttcgtt
     4081 tgactcgttt cactttccaa atacaattgt tcttc
//
LOCUS       DAFBQW010000133         4074 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFBQW010000133 DAFBQW010000000
VERSION     DAFBQW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.139"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(77..1051)
                     /locus_tag="LK889_002940"
     CDS             complement(77..1051)
                     /locus_tag="LK889_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK5097110.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="LK889_002941"
     CDS             complement(1500..2915)
                     /locus_tag="LK889_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBK5097111.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSA
                     VVAENKKLKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="LK889_002942"
     CDS             complement(3137..3499)
                     /locus_tag="LK889_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097112.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3503..3850)
                     /locus_tag="LK889_002943"
     CDS             complement(3503..3850)
                     /locus_tag="LK889_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097113.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQI
                     GVIYRNKSKGKEL"
     gene            complement(3921..4061)
                     /locus_tag="LK889_002944"
     CDS             complement(3921..4061)
                     /locus_tag="LK889_002944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097114.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1153 a          896 c          576 g         1449 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatctaag ctttcataaa
      361 ccatagcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgcc gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacacat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgataaga accatctgat aatttttcaa aactattgag tggcacagga
     1981 tctttttgaa atacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaatg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc
     2821 caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta aatttactct
     3181 cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat tcattgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca aaagattcaa
     3361 atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca atcaatcgtt
     3421 ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt gctttaatat
     3481 ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt cgataaataa
     3541 caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat tccttttcag
     3601 attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc aaagaagaat
     3661 ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca agcatttcac
     3721 tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac atcaattcaa
     3781 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     3841 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     3901 tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     3961 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     4021 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DAFBQW010000134         3739 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFBQW010000134 DAFBQW010000000
VERSION     DAFBQW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.140"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            11..478
                     /locus_tag="LK889_002945"
     CDS             11..478
                     /locus_tag="LK889_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097115.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="LK889_002946"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="LK889_002946"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBK5097116.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="LK889_002947"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="LK889_002947"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBK5097117.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..3697
                     /locus_tag="LK889_002948"
     CDS             3200..3697
                     /locus_tag="LK889_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBK5097118.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1233 a          596 c          807 g         1103 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
     3661 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
     3721 aagagaaatc aagaataaa
//
LOCUS       DAFBQW010000135         3723 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFBQW010000135 DAFBQW010000000
VERSION     DAFBQW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.141"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            131..505
                     /locus_tag="LK889_002949"
     CDS             131..505
                     /locus_tag="LK889_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK5097119.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LK889_002950"
     CDS             complement(619..1248)
                     /locus_tag="LK889_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5097120.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LK889_002951"
     tRNA            complement(1382..1454)
                     /locus_tag="LK889_002951"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LK889_002952"
     tRNA            1668..1741
                     /locus_tag="LK889_002952"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LK889_002953"
     CDS             complement(1879..2043)
                     /locus_tag="LK889_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097121.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LK889_002954"
     CDS             2338..3666
                     /locus_tag="LK889_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBK5097122.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT         1269 a          581 c          697 g         1176 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agc
//
LOCUS       DAFBQW010000136         3525 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFBQW010000136 DAFBQW010000000
VERSION     DAFBQW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.142"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(12..392)
                     /locus_tag="LK889_002955"
     CDS             complement(12..392)
                     /locus_tag="LK889_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5097123.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIIENETEDKKVFPSYFN"
     gene            complement(389..775)
                     /locus_tag="LK889_002956"
     CDS             complement(389..775)
                     /locus_tag="LK889_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097124.1"
                     /translation="MAMIKIRTTITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREAE"
     gene            complement(756..1061)
                     /locus_tag="LK889_002957"
     CDS             complement(756..1061)
                     /locus_tag="LK889_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097125.1"
                     /translation="MNDFHEAMLMANVPTNMGDVYKKAIEAENSPTGLRNNWNGTYAN
                     VQVDKDNRGIAMDVLVISLLSHTLPNLQETVEWYERMGAKVISTNYKGENQNGNDQN"
     gene            complement(1366..1509)
                     /locus_tag="LK889_002958"
     CDS             complement(1366..1509)
                     /locus_tag="LK889_002958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097126.1"
                     /translation="MRIGNVYLISKVPYQKLLYSCKVRFIASDLRNLRKQFILSDADE
                     LAD"
     gene            complement(1834..2301)
                     /locus_tag="LK889_002959"
     CDS             complement(1834..2301)
                     /locus_tag="LK889_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBK5097127.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTANHDFEAERMLIDKEEAQEEFEYLHKLFIRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(2375..2815)
                     /locus_tag="LK889_002960"
     CDS             complement(2375..2815)
                     /locus_tag="LK889_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5097128.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(2845..3138)
                     /locus_tag="LK889_002961"
     CDS             complement(2845..3138)
                     /locus_tag="LK889_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097129.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIKS"
     gene            complement(3138..>3525)
                     /locus_tag="LK889_002962"
     CDS             complement(3138..>3525)
                     /locus_tag="LK889_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBK5097130.1"
                     /translation="RKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQS
                     TGMKDKNGVEIFEGDVVSVSVRNGFDYLDNKVCIVKNSIGHSGLVCATVDEDLEYRIF
                     NTELFEEYTYEVIGNIYENSELLEEK"
BASE COUNT          987 a          759 c          564 g         1215 t
ORIGIN      
        1 ataggggggc ttcaattaaa atacgaaggg aaaacttttt tgtcttccgt ttcattttca
       61 ataattggat gacattttga acataaaagc atgagattgt ttggatcaag cttaagcagt
      121 tcattgtctt tgatcggtac aatgtgatgg acgtgtgccc tcttgccaaa gatgaactga
      181 ccgcatcgct gacagtgacc accttctctt tcataaataa attgacgcat atctttccat
      241 gcctgtgttc gatagaatgg tttgttctca tgatgataaa cagactttgc ttgctgcttc
      301 ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt agataccctt tgctattttg
      361 ttcgtgcatc cgtcaaactg acagtatttc attctgcttc acgaataaga tttatgatat
      421 cgccttttgc acggatagca ccaggaatat caataccatg tttcttagca tatgcacgca
      481 attcttttgc agtcatgttg tccagttcat ccgtatcttc agtggactga tcattagtag
      541 cttcgtctcc atcaaaatca gcagcagtgt ttccatcgct atcaagaatt tcactgctat
      601 tagtaataag ttcaccatta acagccacaa acgttttacc atcacttaat attccttttt
      661 cttccgttgc ttcaaaatca ggttcttgac ctttcggaac tacgacagtc tttttctttt
      721 ctgaatccca atactctgtt cctgtgatgg ttgttctaat tttgatcatt gccattttga
      781 ttctctcctt tgtaatttgt gctaattact tttgcaccca ttcgttcata ccattcaaca
      841 gtttcctgca aattgggtaa agtgtgggat aataaagaaa taaccaacac atccattgct
      901 atcccacgat tatctttatc tacttgcaca tttgcatagg tgccgttcca attgtttcgt
      961 aaaccagtag gactgttctc tgcttctatt gctttcttat agacatctcc catattcgtt
     1021 gggacattcg ccataagcat tgcctcatga aaatcattca tagataaaac tcctttcaaa
     1081 ataaaaagac cactcaaaga gtgatctaat atgtaatagc aacctacaca cagacaagtc
     1141 taatacttcc tgcgcctcac cactgccaca tccctcggtt gctaacgaat actgaaacag
     1201 aataccgtct ctgttcatga ccaacgcgac agtacaaagc tttgtcgcaa gcaggttatc
     1261 gtattcctaa taaccccaac taatgtttct ctcctaacct acacctgaga gtggcgcacg
     1321 tctgcgctag tattttatgc cttctaactg ctagtagcat cccgactagt cagccaattc
     1381 atcggcatcc gataagatga attgtttccg taggttcctt aagtcactgg caatgaatcg
     1441 aaccttgcat gagtacaata atttttgata agggactttt gatatcagat atacgtttcc
     1501 gatacgcatg tactctctcc ctcaatgcgt ctaccctttc cgccacaatg acataatgac
     1561 aatagacagc aacggatgat agataataag aacaatttag aaggagttgg aattcacatc
     1621 cttattctta atatttccgt cgctatctat cgaagcttaa ttgtgaaaca ataataaaac
     1681 gatgttcctt ttattattat tttgtctcag acctatcact aatctttcga cactatcata
     1741 atacaacatt gaataggtaa gtgattggta taaaaaaggt ataaaatgga aaccaaattg
     1801 gtaataaaag ggtataaaaa gtgtaaaaac tggctacttg aaagcaacca gttctaacga
     1861 tgaagcaaat tggatgataa ttctgtttga ttctacttta accgattctt cgctagtatt
     1921 attcctttga gcagttacat aaatgggcag accattgata taacgatcat agaatatctt
     1981 cttgcgcctt tcagtcacat cgggtttgtg cggatgctgt atagcagagt atcctctaat
     2041 aaacaattta tgcaaatact caaattcttc ttgtgcttct tctttatcaa tcaacattct
     2101 ttcggcttca aaatcatgat tagctgtaga tggtggaacc aaggagtatg atgctgtcac
     2161 ttttggttct cgaggttgcc ctactctaca tctagctgat agatatgctg aaagaaacac
     2221 agcgacatta tgttttgtgc gttccatatc aacatctttc gcgcttggtg tctcatattt
     2281 ctttacatcg aaaagtacca tcctctgatt cccccaatta tgatataata cttatgtcgg
     2341 aaatattatt catagtcgga gcgatccggc ttttttattt tgtacgtgaa atgagttctc
     2401 ctgatttata cgcctcagca aattcaacca aagctactgc cttcattctc tcgatagttc
     2461 tatcggaata tcctaatttt tctcctattt gatacatcga catcttatta ggaaaacaat
     2521 aactataata aagtacctgg aaatggattc tacttagccg tgttaatgct tcgataaccg
     2581 aatctcgagt acttatcgct tcaatgattt cttcgttttg actgtcaatt gtgaatttca
     2641 aatttttggt aacagtcatc acggaaaagt ctattttcac tggtccgacc aaacgttcta
     2701 aatttctgta tctttttagt aatcgtctag catttttcct tgtttgctta atatctatat
     2761 tctctaaaat gttcacaatt gcctcttctc cttccagttg tggaataacg ctcattgttg
     2821 taggatagtt gtaaaaaagc aaaattaaga ttttataact tccatatcca ccaatctcgc
     2881 tacagctaaa ttctctttgc ttttcgctaa ccacttgtca cattccatcg tgttttcaat
     2941 gcggatgatt gctgagtgat tatagagatg ctctacatat ccacgaaacg gatagatgaa
     3001 ctcctctgct tcacagcgaa ccatgtcacc gactttgact tttggtttct tacgtgtttt
     3061 agggttcttt gtcggcatat ctagcattaa accgccgata ccatgactac tagcgtaaaa
     3121 tccgtctttt agtttcatta tttctcctcc aataactcgc tattctcgta tatatttccg
     3181 atgacttcat acgtgtattc ttcaaacagc tctgtgttaa aaattcgata ctctaaatct
     3241 tcatcaacag tggcacaaac taatcccgaa tgtcctattg aatttttgac aatacaaact
     3301 ttattatcta agtaatcgaa tccatttcgc acgctgactg atactacatc cccttcaaat
     3361 atctccacac cattcttatc tttcattcct gtggattgca tgaggtcgat gtttcgcacg
     3421 tgatatgatt tcaattcggt aggatttatc caatattcaa taaagtttgt tttgccgttt
     3481 ttagtaaaat gcaagacggc tacatctctc attacgttct ttcgt
//
LOCUS       DAFBQW010000137         3460 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFBQW010000137 DAFBQW010000000
VERSION     DAFBQW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.143"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(578..1594)
                     /gene="tsaD"
                     /locus_tag="LK889_002963"
     CDS             complement(578..1594)
                     /gene="tsaD"
                     /locus_tag="LK889_002963"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK5097131.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1591..2055)
                     /gene="rimI"
                     /locus_tag="LK889_002964"
     CDS             complement(1591..2055)
                     /gene="rimI"
                     /locus_tag="LK889_002964"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK5097132.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2062..2604)
                     /gene="rimI"
                     /locus_tag="LK889_002965"
     CDS             complement(2062..2604)
                     /gene="rimI"
                     /locus_tag="LK889_002965"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK5097133.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2588..3310)
                     /gene="tsaB"
                     /locus_tag="LK889_002966"
     CDS             complement(2588..3310)
                     /gene="tsaB"
                     /locus_tag="LK889_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK5097134.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          989 a          757 c          594 g         1120 t
ORIGIN      
        1 atatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg cagttttaga
       61 tcactggaca agactcaaag attttctatg tgtgtttcag ttatgtaata ttcactgttt
      121 gcaaaaaata ctgttagatt aactagatga agcgtcttgt cctcatccga tctagattat
      181 tcgatattcc tcgacaataa acgattactc agtgagggca atcagaggaa cggataatga
      241 taaataagct gtaaaaaata gcaaaataac aataatgatt aaaagtatgt aaaaaatagg
      301 gctttgacaa aaacgtgtca cagccctatt tcgattaaac ggtgtgtacc tgtcaactct
      361 tcggtcacaa gtcataatgt tcataaacat gaggagctgt tttttgaaat tgttccttgt
      421 gaatcagagt taaacgacag gcttacgaac tcttgattaa acggcgttca aaagcttgca
      481 tacgcggtaa taagaacagc caggtcaaaa acatgagaag ccgtttttcc tgggctgttc
      541 ttattacttg atgcagggca gctttttcac aacctcttta ttcagataaa accaagcttg
      601 gatcagcatt caactgatag ctagcaaaat ttcttttttg catttccaca tgtgctgctg
      661 ctccaatcat tgcagcatta tctccacaaa gtcgaagagg gggaatcact agttctacct
      721 cagggagttt agttgccaaa gcatcctgca atccttcacg caacccttga ttggccgcta
      781 cacctccagc tataatcagt tgcttgactg gataattttg acaagctcga agtgttttag
      841 taatcaatac atcgatcaca cttgcttgaa agctagctgc taaatcattt ttgtctagat
      901 cttctccacg ctgttgtgca ttatgcacca aattgataaa tgcacttttt aatccgctaa
      961 aactgaaatc ataattatct tcatggatca tcgctcgcgg aaaatggtaa ttatcttttc
     1021 cctgatgagc tagctgatcg atttcttttc cgcttgggta agaaagacct aatactcttc
     1081 caactttgtc ataagcttct cctgctgcgt catctcttgt ctccccaatg atttcatatg
     1141 atccatcttc ttgcatatag actaattctg tatgtcctcc gctaacaaga agtgccatca
     1201 atggaaactg gaatggttta accagacggg ctgcatagat atgccctgcc atatggttta
     1261 caggaataag cggcaattgg tgtgcccaag caaaagcctt agctgcagaa ataccaatca
     1321 gcaacgagcc gacaagtcct ggtccataag tgacagcgac tgccgataaa tcttctgccg
     1381 acacaccagc ttctaccagt gcatcttcca agcataaggt gatttgttct acgtgatggc
     1441 ggctagctac ttcaggtact acgccaccaa aacgtttatg actgtttatt tgagaagcaa
     1501 cgatgttaga taagatctct gttccatttc taacgaccgc tacactcgtt tcatcacaac
     1561 ttgattcaat cgccagaatc aattcttcat tcatttcatc gtctccgttt ttaactttct
     1621 gctcataatg atcgcatctt ccgtcggatt gcgataatat gatttccgtt tacctaatat
     1681 acgaaacttt tctgattcat aaagttttct tgctgcttca ttagatagac gtacttctaa
     1741 aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc
     1801 atgccctttt ccttgttctg aaactgcaac agcaatattt gtgatctcga cttcatctat
     1861 tactttcgag tatccaataa agccacagat ctggttatct tcagctaaaa gcagataatc
     1921 agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg
     1981 ataagcattt tcgctaatca tccatacgca ttctgccaag tacgcttcat caaaatcttt
     2041 ttttctgcaa atcaactaat ctcactcatt ttctaaaagt tttatcatat ccaacgcatc
     2101 ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt gcttgaaacc ccaatttccg
     2161 ataaagtctt tgtgcatctt gattactcat acgcacttct aatgacatcg tctcacagcg
     2221 attcattata gcaaagttct cgatttcgtc aataagcagc gaaccgattc gtttacgctg
     2281 atatgccggt cgaacggcaa tattcgtgat atggcaatct aaaccgaacg ctcttgaacc
     2341 gataaaacca atcattcttc cattatcaaa gatacctaga tatagatgag gaagcggtga
     2401 cttcaattct gatataaagg cactttttgt ccatggcaat tcgccattat agacttctcg
     2461 ttcgacatcc agcagctctt tgatatcttc tgtagtcaat tctcttagta cgtaacgacg
     2521 atcgatttgt tcgactgttt tttctgtata aatcatttta tcttgaaaaa agcttctggc
     2581 atacgctcta aattttttca acataacttt cgtctccagg tgtgtgcgtc tccaaccatt
     2641 tttcttctgc ttctactcgt tttaaatagc gaggaagaaa agcttgtttc tctgcaactg
     2701 gttcaactaa agcacctaat tgagccacag ttacaccact tggaatatcc catgctggaa
     2761 ctgtattaat catcgcacta ggcaatattt cctttatttc agagaagaat ttgtgacaat
     2821 cactcccgac gaaataaata gcttcattca gcttttgtag ttgtgtgaac aaatcagaag
     2881 cagaaatatg tacatcttca agtacagttt ctaatctatt ttctttccag cggtatgccc
     2941 cagcgtatac attttttctt cgagcatcaa acatgggcac aataatatta tccacaccga
     3001 tgcaattagc cgcgatggtt ttcagactag atactcctac tagctcttta tctaatgtat
     3061 aagcaatcgt ttttgctgtc gttacaccta tacgaagccc tgtataagaa ccgggaccat
     3121 cagaaaccgc gattcggtca atgtcttttg gacttatttg aagatctgca aacaattggt
     3181 cgattgcagg catcagtgtt acactgtggt tgcgtttgat atttgtttgt atttgtccaa
     3241 gaatttgttc gtcttctacc acaccgatcg ccaaagtttg gtttgcagta tctattccta
     3301 atgtaatcat atatttgatc ctttctgcta tgacattctc gccttccatt gtagcatact
     3361 tttttattct gaggatgaaa ataccaaagg caaaactagt acaataatac atgattatct
     3421 gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa
//
LOCUS       DAFBQW010000138         3280 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFBQW010000138 DAFBQW010000000
VERSION     DAFBQW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.144"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(366..1484)
                     /locus_tag="LK889_002967"
     CDS             complement(366..1484)
                     /locus_tag="LK889_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097135.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="LK889_002968"
     CDS             1955..3262
                     /locus_tag="LK889_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK5097136.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         1032 a          680 c          590 g          978 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt caacacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac
//
LOCUS       DAFBQW010000139         3213 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFBQW010000139 DAFBQW010000000
VERSION     DAFBQW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.145"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            46..1434
                     /locus_tag="LK889_002969"
     CDS             46..1434
                     /locus_tag="LK889_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBK5097137.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1772..>3213
                     /locus_tag="LK889_002970"
     CDS             1772..>3213
                     /locus_tag="LK889_002970"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK5097138.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEE"
BASE COUNT         1181 a          504 c          582 g          946 t
ORIGIN      
        1 aaaaaaaacc aaccgaaaga tataaaagat attgtagata cacgaatgta taaagaacgt
       61 gatgacttaa actatacgct tattatcaat aaagatggaa actattcatg gcgtcctctc
      121 gttctactac atcctattct atatgtagat ttggttaatt gtctcactaa agaagaccat
      181 tgggaggagt taaaaaagag gtttagtgaa tttaaaaaag ataaaaatat taactgttat
      241 tctatacctg ttgaagcttc caaaggttca aaaaggacag atcttggtga aacaatacta
      301 aactggtggg aatattttga acaaaaaagc ttagctcaga atattaaata ccaatattgc
      361 atgtttactg acatcgctga ttgttatcca tcaatttata ctcactctat aacatgggcc
      421 atgtatggta aagaaaaagt taaaaatgat gaaaataagt gttcgggctc acttggcgat
      481 aaaatagata aaaaaatatc tggaatgcaa cacaatcaaa caaatggtat ccctcagggt
      541 tccgttctta tggattttgt agcagagatc gttctagggt atgttgattt gagattatcg
      601 aaagaattaa aaaaattaga aaatttaaaa gattacaaag tactcagata tagagatgat
      661 tatagaatat tttcaaataa tcgtaatgat ttggaacaaa ttatgaaagt gttatcagaa
      721 gtcttatatg atttaaattt taagttaaat tctagtaaaa caaaattatc agatgatatt
      781 atcatagatt ctatcaaaaa agataagctc tattgggaag aaattaaggc aagtataaga
      841 atgaatgcca caaacttaga tggtgataga gaaattcgtt atagtataaa tttacaaaaa
      901 catttattgg aaataaaaaa gttagcagat aagcatccta attcaggcca attgaaaaaa
      961 gcactttctg agtttaataa agaacgcatt attaaaatca cttcaaagcc gaacgatctt
     1021 ttaccactta ttggtatcct ggtagatatc atgataaata acccgggttc tttgccgcag
     1081 tgtgtcttaa ttatagggca gttattaaat ttcgaaaatg agagtaaggc tagagaaata
     1141 gttgaaatga ttataaacaa atatgcttta aagccaaata ctgaatttat gatgatatgg
     1201 tttcaaagat tgaccaccat ttttggagaa atgtttgata gtgttttatc atccgaattt
     1261 gtatctaaaa tcaaagatcc tgtctctaat cagatgtttt ctgtagactg gctaaatgaa
     1321 gatgctaaga aatcttttta tgaagggacc attaaagacg aagagaaata tattaaaatg
     1381 agtaagaaaa tatatattac tgaatttgat gattttaatg attatccttt ttaagtgaaa
     1441 atttagaatg aatataaaaa acattacaat ctaatttttt taaaaaaatg ttatatcgga
     1501 agtaatctct tgatttctat actataagat aagaaaacca ctatttaaca aggttattcg
     1561 aaccttattt taagttactt attatttaat gtatcgaaaa aggtgaaata ggtttgttat
     1621 aataagaatt aagtaagaca tgaattgtct aagcaaatgt agcttatcca tttcattaaa
     1681 atacatatgg tgaaaaaaca agttcaaata gtaactaatt ttttaaaaaa ctgatttata
     1741 aaaaatacaa cacgaatgag gaaaataacc aatgacagaa caaatcgaaa aaaattcaaa
     1801 aagcttatat caggcactgt ggaattccgc agatatcctg cgttcaaaaa tggacgctaa
     1861 tgaatacaag agctatttac taggcttggt cttttataaa tacttatccg acaacatgct
     1921 gcggtatgtc tccgttctat tggaggaaga aacggacgac ctaaataaag cacaagacct
     1981 ttacgttgaa gcatttaaag atgcagatat aaaggatgat ctcttgtatg aactaaaaga
     2041 tgagttttca tataccatcg cacccgcatt gacctttacc gcgcaagtgg cagccattca
     2101 tgatggatct tttcaattag aagacctggt acaaggtttc cgtgacattg aacaatccag
     2161 cgaaaacttt gagaacttat ttgaagacat tgatctgtat tctaaaaaac tggggtcaac
     2221 cccacaaaaa caaaataaaa ccatcgcaga tgttatgaaa gaattagaag ggttaaacat
     2281 ggcggggcat gctggcgatg tcttaggcga tgcctatgag tacttaattg gccaattcgc
     2341 atctgaatcc ggtaaaaaag ccggtgaatt ctatacccca caaccggtcg caaaattgat
     2401 gacacaaatt gttttacaag gaaaagaaga taaaaaaggc ttttctgttt acgacgcgac
     2461 catgggttcg ggctcgttac ttttgaacgc taaaaaatat tcccatcaac cgggaaccat
     2521 ttcttatttc ggacaagagt tgaatacgtc tacctataat ttggcgcgta tgaacatgat
     2581 tttgcatggg gttcccattg ccaaccaaca cttgcataat gcggatacct tggatcaaga
     2641 ctggccaaca gaagagccaa cgaacttcga tggggtgctc atgaacccac cgtattctgc
     2701 aaaatggtcg gctgataaag gcttcttaga tgacccacgc ttctctgcct atggcgtatt
     2761 agcccctaaa tcaaaagcgg actttgcttt cttattacat ggctactatc atttgaaaga
     2821 tacgggcgtc atggccattg ttttgccaca tggggtgctc tttagagggg gcgcagaagg
     2881 gaagatacgc aaagccttgt tagaaaatgg tgccattgac accgtcattg gcttacctgc
     2941 taacatcttc tttaatacgt ccattccaac gaccgtaatt atcttgaaga aagaccgtgc
     3001 gaaaaaagat gtgctcttta ttgatgcgtc acaagatttt gaaaaaataa aaacgcaaaa
     3061 tacgctacga gattaccata ttgacgctat tttagaggcc tataaaaccc gtacaattaa
     3121 agacaagtat gcttatgtcg cagaatttga tgaaattgtg gagaatgatt acaacttaaa
     3181 cattccccgt tacgtggata cctttgaaga aga
//
LOCUS       DAFBQW010000140         3179 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFBQW010000140 DAFBQW010000000
VERSION     DAFBQW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.146"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(7..978)
                     /locus_tag="LK889_002971"
     CDS             complement(7..978)
                     /locus_tag="LK889_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK5097139.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1684..3063
                     /gene="brnQ"
                     /locus_tag="LK889_002972"
     CDS             1684..3063
                     /gene="brnQ"
                     /locus_tag="LK889_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK5097140.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT          894 a          634 c          593 g         1058 t
ORIGIN      
        1 caaagcttat tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac
       61 agggtgccga acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt
      121 taatgttcgt tctgcatgaa caagaccact tgttgtccct cgaggcagat caatctgact
      181 cgtatctcca ttgacaatca tttttgattg ataaccaaga cgtgtcagga acattttcat
      241 ctgagcaact gtcgtgttct gtgcttcatc caaaataacg aatgcatcat ccaatgtccg
      301 cccccgcatg taagctaaag gagcaatttc gatcacgcct cgttccatca atcggttcgt
      361 atgatctaat ccaaaaattt ggtataaagc atcataaaca ggacgcagat aaggatctac
      421 tttttctttt agatccccgg gaagaaagcc tagattttcc cctgcttcca cagcaggccg
      481 cgtcaaaatg attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc
      541 aagaaaagtt tttcctgtcc cggctggtcc tacgccaaat acgatatcat gtttggctac
      601 cgattcgaca tatttctttt ggccgctgtt ttttacccgt attggttttc cattacggtc
      661 tttgacgatc tcttcctcat acatttcaac aaaataatct aatgtgcctt tttgccccat
      721 tttcagtgcc gtcacgacat ctggtgtact tacatggatt cctcgtttga tcaattcctg
      781 caatgtccga atgactgagg cagccaagga aatctgggat tgttcgccaa tgatctgaat
      841 gatttctcca cgtgtatgga tggttgtttc tgttgtttct tcaatcagtt ttatatgctt
      901 gtcgtgtgca cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga
      961 actagactct tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt
     1021 accatactca gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc
     1081 ataagtacca agaaacgtta aaaaatagct gcttccattc tctaaaggtc tcgtttgaat
     1141 aaaaaaagct gatcatgtaa gctatgcata agatagagga caagtattcc gtctaactta
     1201 gcaagtattc tctgggtatt tttcttgttt caaaaaaaga aaacaaaata tttgaaatat
     1261 tcttgcattt caaaaaaaac aggtgtatta taacgtcaac aaataaatat acagcaaagc
     1321 gtcgaggaaa acagtaggct attcagcgat ttattcccag tgagtcatgt aagtgagaaa
     1381 tgacaataaa tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt
     1441 gtggctgcct ccattatcag gctagcggat aactttatcg aaaagcgtcc gtgaatgaag
     1501 aagctttttt aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt
     1561 ggaaaagggc ttttttttat attttaaggt ggtggttttc atggaatctg atattagtgg
     1621 catttctagt ttctgcaacg tttattttgt tactcaaaat gataattatt ggaggaaaaa
     1681 tttatggaag aaacaaaaac ttattcatgg aagcaattat tgatggtcag ttcacttatc
     1741 ttcgggatgt tcttcgggtc aggtaatctg atctttcctg tccacttagg tcaaatggct
     1801 ggatcaaact ggatcagcgc tggtgtagga tttgccatct caggcgctct tttcccatta
     1861 ttagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct
     1921 gtcggaaaat ggtatgctgc cttgttcttg atccttgttc atttaacgat cggaccattc
     1981 tttggtacac cgcggacagc agcaactgct tttgaaatgg ctgctaaacc ctttttgcct
     2041 gaaaaatatg attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat
     2101 ttccttactg tacatccaac acgtttggtc aaatatgtcg gaaagtattt aaactccttg
     2161 tttcttgcat tattagctat catttttgtc gtttctttat tccaacccat ggggaatcta
     2221 aaccaatctg ctgcagattc ttatcagacg aacgctttga caaatggcgt tttagaagga
     2281 tacaacacgg ttgatgcagt agctttactt gctttaagtg tcacttttgt ccatgcagtt
     2341 cgtgatcttg gttttaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg
     2401 agcgttgcgt tagaagtcat gatttatttt ggtctggtcc ttctcggtgc gatgagtttg
     2461 aataagatgc ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat
     2521 ttaggtaact tcggtgctag cttcttaggg gtaatggtca cattaggtgt cttcactacc
     2581 gctatgggac tcgttgtttc ttttgcacaa gatttccata aactgttccc taaagtcagt
     2641 tatatgacat ggctgcggtt aacgactttt gtctcttttg tcgttgcgaa tgccggattg
     2701 gataatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcacttgcg
     2761 ttaatcttgc tttccttaac agcaaaattc ttccaaaaga gtccatttgt ctatcaagta
     2821 acgatgttct tttctgccgt tccagccgta ttggatatgt tggcaaattc gcctgcatta
     2881 gtgagtcagc aaactgtcgt agcaagctta ctaggatttt atcatcatca cgttcctttt
     2941 gctgcattag gacttggctg gatggtccca acgttacttg gttacgctgg cagcttactc
     3001 ttttattacg cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa
     3061 taaccaaatc aaacacaatt taacataaga gatattaaac aggaaaaaaa cagctagaag
     3121 aaagaataag attctttctt ctagctgttt tttagttctt tttttacgaa cacaaaaaa
//
LOCUS       DAFBQW010000141         3082 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFBQW010000141 DAFBQW010000000
VERSION     DAFBQW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.147"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(45..779)
                     /locus_tag="LK889_002973"
     CDS             complement(45..779)
                     /locus_tag="LK889_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK5097141.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="LK889_002974"
     CDS             1106..1516
                     /locus_tag="LK889_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK5097142.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..1709
                     /locus_tag="LK889_002975"
     CDS             1509..1709
                     /locus_tag="LK889_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBK5097143.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIDVHPKS"
     gene            1741..3080
                     /locus_tag="LK889_002976"
     CDS             join(1741..2182,2182..3080)
                     /locus_tag="LK889_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBK5097144.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         1120 a          476 c          568 g          918 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatattga tgtgcacccc aaaagttaga ctagaaatct aattaaaagg ggtgcttttt
     1741 ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt aaataacgaa
     1801 ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt tagacattgg
     1861 gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa aagctacact
     1921 acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc tatccaagaa
     1981 actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat agagcaatat
     2041 gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat tagtatgaat
     2101 tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga agttattcgt
     2161 ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga acaagatttg
     2221 agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt aaattgaatc
     2281 tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta aaaaaattag
     2341 ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta aaagaatcaa
     2401 gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga tatacaatca
     2461 atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa atccgaataa
     2521 agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg ttaaaacgaa
     2581 atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa ttcaagataa
     2641 aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa ataattagtt
     2701 atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa caagcaatta
     2761 aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg cagtatcaga
     2821 tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg tctagaaaag
     2881 gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa tcagagtttt
     2941 tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat gagtatatta
     3001 ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca gtagaatatc
     3061 ggaaacagtc cattaaatag tt
//
LOCUS       DAFBQW010000142         3070 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFBQW010000142 DAFBQW010000000
VERSION     DAFBQW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.148"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(334..696)
                     /locus_tag="LK889_002977"
     CDS             complement(334..696)
                     /locus_tag="LK889_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097145.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="LK889_002978"
     CDS             complement(770..1150)
                     /locus_tag="LK889_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBK5097146.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="LK889_002979"
     CDS             1214..2407
                     /locus_tag="LK889_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5097147.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="LK889_002980"
     CDS             complement(2484..3068)
                     /locus_tag="LK889_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5097148.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          958 a          587 c          504 g         1021 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat
//
LOCUS       DAFBQW010000143         2993 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFBQW010000143 DAFBQW010000000
VERSION     DAFBQW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.149"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..420
                     /locus_tag="LK889_002981"
     CDS             <1..420
                     /locus_tag="LK889_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097149.1"
                     /translation="EGVTGRKISLKDESSTTVSDANILANIILDFIFEHDIPFKNGYE
                     ILPQNEQYFFYKCLTNRVCCSCGKKNADIHHVDSVGMGNNRKKINNSGRRFMALCREC
                     HTKIHVEGFTTFTTKRKLTAVVLKDSDLKRLGLNIIE"
     gene            428..943
                     /locus_tag="LK889_002982"
     CDS             428..943
                     /locus_tag="LK889_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5097150.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            1052..1903
                     /locus_tag="LK889_002983"
     CDS             1052..1903
                     /locus_tag="LK889_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBK5097151.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPPKKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYQKKKALERAN
                     RQSKAPF"
     gene            1915..2184
                     /locus_tag="LK889_002984"
     CDS             1915..2184
                     /locus_tag="LK889_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097152.1"
                     /translation="MKIDYLELINEIASYKTGEEIEILRDVYEQLDEAGIERIKNDRS
                     SWSKLRYYFALYIDATQLRNLAYTKLLFADCVKGLQKHLSELEQV"
     gene            2189..2353
                     /locus_tag="LK889_002985"
     CDS             2189..2353
                     /locus_tag="LK889_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097153.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDE"
     gene            2346..2648
                     /locus_tag="LK889_002986"
     CDS             2346..2648
                     /locus_tag="LK889_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097154.1"
                     /translation="MNKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVTGTAEQLASLTGLHEKIVRKRARCGYTDVKKRTFRYVEGSS"
     gene            2645..2806
                     /locus_tag="LK889_002987"
     CDS             2645..2806
                     /locus_tag="LK889_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK5097155.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            2803..>2993
                     /locus_tag="LK889_002988"
     CDS             2803..>2993
                     /locus_tag="LK889_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097156.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGT"
BASE COUNT         1157 a          460 c          659 g          717 t
ORIGIN      
        1 gaaggtgtca cagggcgcaa aataagcctc aaagatgaat cgagcacaac cgtaagtgat
       61 gccaatatct tagcgaatat catcctagat ttcatctttg agcatgatat tccgtttaaa
      121 aatggatatg aaattttgcc ccaaaacgaa cagtatttct tttacaaatg cctcacgaac
      181 agggtgtgct gtagttgcgg aaagaaaaat gctgatatac atcacgtaga ctctgtcggc
      241 atgggaaata accgaaaaaa aataaacaat tctggtaggc gctttatggc tctgtgcaga
      301 gaatgccata cgaaaattca tgtcgaaggt tttactacat ttacaactaa aagaaagctt
      361 acggcagttg ttctcaaaga cagtgattta aaaagattgg ggttgaatat cattgaatga
      421 attatggatg gatatcgaag gatacgaggg agtatatcaa gttagtagct taggaagagt
      481 taaatctaat cgccctaact atgtaattaa taagggatac gttaacaaag aaggatttat
      541 tatgaaacca tctgacaatg ggaaagggta tcaaattatc ttcttaagcg gcaaaggttt
      601 tagagataga aggtatgtcc atcggctagt agcttttcac tttttaaaag aagcgtattt
      661 cgaaggtgct gaagtcaatc ataagaacgg agataaatct aacaatactg ttgaaaacct
      721 agaatggctc agttctgatg ataataaaaa gcatgctaga aaaaatggat taactaactt
      781 gaaaggacca tcaaaactaa ctgatttgca agctttagct atcaagcgtt tatatacaaa
      841 caaattgatg agctccggag aaataatgaa actttttaat gtcagcaggc ataccgtgct
      901 gaatatagct tcagggaaga catttagttt cttggaagat taagagaaac acaaaatagg
      961 tgtgactaat ttcaaaaata agtatcaaat caaagggatt aagttaaacc aggaaacaat
     1021 taagaaactt agaataggag gataaaaaat attggctgac aacaaacgat actactattt
     1081 aaaactaaaa gagaacttct tcgatagcga cgagatggtt cttttagaaa gtatgccaga
     1141 tggatacatt tattctaata ttcttctcaa actttattta agaagtctga aacatgaagg
     1201 caaactaatg tttaatgaca ggattccatt caattctaca atgcttgcga ctattacaag
     1261 acactctgtg ggagttgtag aaaaagcagt acaaatattt cgtgatttac aacttattga
     1321 ggtattagat aacggagcaa tttatatgtc tgatatacaa agttttattg ggaaatcttc
     1381 aactgaagct gataggaaga gaaaatacag aaaagaaatt gaggaagcaa aacagaattt
     1441 aataacaagt ggacaaatgt cggacaaatg tccggacaaa actacaccag agttagagat
     1501 agagttagag aaagatatag agttagagaa agatatagag aatgtaacgc ctccgaaaaa
     1561 atcgaaggct aagcccatcc gtcataaata cggagagtat aaaaatgttc ttttgtcaga
     1621 tgagcagatg gagaaactca aaacagaatt ccctaatgac taccaagagc gaatagaacg
     1681 gctatctgag tattgtgaat catcaggtaa gacttataaa aactatttgg caactattcg
     1741 aagttgggca aggaaagaaa aaagtgagcc taagaatgca agtggtgcat acaaacgcac
     1801 aggacgacga gagaagcttc cagaatgggc aatcgaccaa gaagcctatc aaaagaaaaa
     1861 agcgctagaa cgagctaata gacaatcaaa agcaccattc taagaggtgg aaaattgaaa
     1921 atcgattatc tagaactgat caatgaaata gcaagttaca aaactggtga ggaaatagag
     1981 attctgagag acgtttatga acaacttgat gaagctggaa tcgaacgaat taagaatgat
     2041 cgttcaagtt ggagtaaact cagatactat ttcgcacttt atatcgatgc aacacaatta
     2101 agaaatttag cttatacaaa attactattt gctgattgcg ttaaaggatt gcaaaaacat
     2161 cttagtgaac ttgagcaggt gtaatcagat ggatctaaaa acatttacag cacagatcga
     2221 actaatgcat caagaagctt taagacaaag cgcctcgtac gaagacaagt ggctcaacac
     2281 gttccatggt ggacgtgaga gcgcacttga tcaagtgctc aaattattga aaggagaatg
     2341 tcgggatgaa taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
     2401 ggcaaaatga caaagaagct gcttcagaag tgatgagact tggaagagcg atgtgggcag
     2461 acaagagcaa caagccaaga ccgcgaaaaa tcgcaatttg gcacggtgac aaacttctag
     2521 tgacagggac agctgaacag ttagcaagtc tcacaggctt gcacgagaaa atcgtgagaa
     2581 aaagagctag gtgtggatac acagacgtta agaagagaac gtttagatac gtggagggat
     2641 cgtcatgaca acagaagaag tgattcaaat gcgtattcga aacattcagc gtgaaattga
     2701 cgatctagaa cgaacaaagg cagtgatggt caatgaaacg gctagaaagg caatcgattt
     2761 gcacatagag aacttaagaa gggaaattcg gagattggag gaatgagcgt ggataagaaa
     2821 gcagcaatga aacgaatcat cgaactgaca cattctgaga attggcaaga agacaaagaa
     2881 atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga
     2941 acgccgagaa aaattgcaat ctggcatgat gaccgaattt tagtaacagg tac
//
LOCUS       DAFBQW010000144         2987 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFBQW010000144 DAFBQW010000000
VERSION     DAFBQW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.150"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            287..694
                     /locus_tag="LK889_002989"
     CDS             287..694
                     /locus_tag="LK889_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097157.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="LK889_002990"
     CDS             708..1109
                     /locus_tag="LK889_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097158.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="LK889_002991"
     CDS             1111..1482
                     /locus_tag="LK889_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097159.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="LK889_002992"
     CDS             complement(1518..1820)
                     /locus_tag="LK889_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097160.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1916..2710)
                     /locus_tag="LK889_002993"
     CDS             complement(1916..2710)
                     /locus_tag="LK889_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBK5097161.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT         1053 a          424 c          458 g         1052 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat cgaatttaaa ccataattgt
     1801 gtttttcctt ccattaacat ataaagttca cctcaaaaag agtatacgaa caaatgtttg
     1861 tattgtaaat agaaaaagat gagctaaatt aaatgcccat cttctttgat agatattata
     1921 aatctaataa ttcttgtttc tttttattaa attcttcttc tgtaatgatt ccatcatcca
     1981 atagttcttt atattttctt atttctgaag ctgaggaaat tgttgatgta ttggaatttg
     2041 tagcagaaat atttcttctt tttaaattat aaataatttc gtttaaagaa tcacttatcc
     2101 tatcaacagt atctttatca actgcaacat tcaatacttt accaagagta tcaatagtta
     2161 atgtacccaa cagcactcct ctttttttac taatattatt taaattgtct aatataatac
     2221 tttgtacatt attgcctatc attttttgtt gagctacaat taaacgttta ttagttaatg
     2281 cgtatgcata attattgtca tgcttagttg atgagatata attatgcaat cctacaaagc
     2341 agaagattac gtattcgtca ctgttaagtt gatctgatat taagttaaaa tgatgagttg
     2401 tccatttttt agtcattcct ttaccgtagc cgtatttaag acaaaattct accatctctt
     2461 cagtggttct cattctatca ttacctatat catgattgtt ttgtgcagta tgatcagata
     2521 tgtttaattt tcttttttct gatggatcct tatctaaatc ttctaaattc aaagatctta
     2581 ttattttata aggagtagtc ataccgtaac cagctttttt catacaattg ccgcaaacaa
     2641 tttgtttatc tttgatttta tatgaagtta agccaatttt acctccgcac tttgcacatt
     2701 gtttactcac attagttcct cctagatatc agctttctac tactattaac attctatcaa
     2761 tagtgaccat actttgacca tactttttag aaagaataga aaattccaag atatttaata
     2821 atagaagaaa agcactaaca tcatgggttc agaagaattg agagcttatc aggaaacagt
     2881 ataattgaag tgtctgttta tccagtagct caagaagctt aattgaatct tatgattaaa
     2941 gaggttgtga caagactttt gtcacagcct tttttgttta taaaaat
//
LOCUS       DAFBQW010000145         2982 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFBQW010000145 DAFBQW010000000
VERSION     DAFBQW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.151"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(50..784)
                     /locus_tag="LK889_002994"
     CDS             complement(50..784)
                     /locus_tag="LK889_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097162.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKGKRIMVPLQSKKTSSASKVRTFF"
     gene            1527..2081
                     /locus_tag="LK889_002995"
     CDS             1527..2081
                     /locus_tag="LK889_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5097163.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            2335..2646
                     /locus_tag="LK889_002996"
                     /pseudo
     CDS             2335..2646
                     /locus_tag="LK889_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2757..>2982
                     /locus_tag="LK889_002997"
     CDS             2757..>2982
                     /locus_tag="LK889_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK5097164.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVC"
BASE COUNT         1121 a          400 c          497 g          964 t
ORIGIN      
        1 tccctttagg aaaacaagat cgtatttgtc cgattttaga aaaagaaatc taaaaaaacg
       61 tcctcacttt tgatgctgag gacgtttttt tagactgtaa tggaaccata attcgctttc
      121 ctttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      181 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      241 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      301 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      361 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      421 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      481 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
      541 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
      601 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttcgatgcct tttaaaacac
      661 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
      721 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagtatat tttgatgaac
      781 gcatatgtag tcctcatttc ttgggagatt tgagactact atatgcgttt ttttgttgat
      841 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtatact
      901 actatatttt aggtttcata ttatataaat taataatata ctaccatttg ttggtagtat
      961 aatttaaagg taataagtta ttcttttatt actatgtttg ggttttatag caaggaaagt
     1021 ttaataagag tttggatcta gcaaacaata ttaatgaggt cattaaatct tattatcttt
     1081 agtgatataa cgaaatatct acacagacaa aattatgatc ttgcgtaaag ttataaaata
     1141 tcaataaaaa tattttatta atgaaaagct tggtagcacg gttattcata tgctaattta
     1201 tttcacttta tagtaagggg agttcttagt atattgttca aacttaaatt tcatataatt
     1261 taattatgta aattagctat tctaagaact tctacggata attgcccgtc taaaatttaa
     1321 ataagaggtt agaatcttca tataagtaag gttaattttt ttgtacaaaa aagtcggaat
     1381 attctgagtt actgaaatga tataaaatca taaactaaac cccttgattt tcttcacttt
     1441 taacagagta tatatataag ttcatgatta tggtaaaata tatatataat catgaactca
     1501 gaatattttt aagaagggaa acaatagtga gctataatgt acaaccgtta aggacaaatg
     1561 aagagataca agattttcag ttttggctaa gaagaacaag taatccagaa agagatagtt
     1621 ttttattttt gtttggaatc aataatgggt tacgtatgtc agatattata ggattaaaag
     1681 ttggagacat acgaggtaaa agtaagccta ttattgttga acataaaaca ggtaagcgaa
     1741 aaccgatttt tatagataat ttgagagaag agattttatt atatactgaa ggtaaagaag
     1801 aaaatgattg gttatttcca agtcggcaac aaggtcgaca tataacaagg gatcgtgtct
     1861 atcagatata tgcagatata gcagaaaaat taggaagaga tgatatagga acacatacat
     1921 taagaaaaac ttttgggtat cactactaca agaaaactag agacattgct accttaatgt
     1981 ttatttttaa tcatagctcg caagcaatta caaaaaggta cattggtata actgaagatg
     2041 aaataggggc atcgttacgt ggttttaaat tgggaggcta attgagatat taggatatat
     2101 tttttgtgta taatgaaaac ttagaaggtg agattaatga agataactga attttcggaa
     2161 gtatttaaaa cgtttagtaa catttacaat agaataccag agatagaaca aaatgttgta
     2221 gttgataatg attattatat agagaatgat tttaataaac caagaaaata tgacaaccta
     2281 tacaatttta tacacaatag aacaacaata aataacaaca gtaacactcg agttttgatt
     2341 aattcagcat attttcatat taaatattta aagaaatatg agtatgaaga agttgtatta
     2401 gattatgggc taaatagaca gtcattttta atttatgcaa taaataacgg aaatttgcca
     2461 agcaataaaa agaacataat tattgaaaca tatgcctatc tggagggggg atctaaaaaa
     2521 ctaattaagt catgtaatat agagtatcaa ttatcagtag gagaaattaa atgtgttttc
     2581 gaagaagatt tagaacttga cttaaaaaaa atttttgcca gcattcatga ttatataggt
     2641 ttgatacccg tggtgctagt taattttttc ctagaggaaa atgtctaaac atgttggtaa
     2701 atcaacaaaa aaacgcatat agtagtctca aaactcccaa gaaatgagga ctacatatgc
     2761 gttcatcaaa atatactgaa cattatacag atacttttat aacattcaag gaaattatga
     2821 gaactgtttc taacatatac cacaattgtg taccagataa gattaaaaac cgaagaaata
     2881 ctgatcaact gaagcaacgc gatacagtga ttattgcttg tgttatatgg ggcataatta
     2941 atggctatac tagccaaaga gccacgtata gagcggtttg tt
//
LOCUS       DAFBQW010000146         2953 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFBQW010000146 DAFBQW010000000
VERSION     DAFBQW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.152"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            89..406
                     /locus_tag="LK889_002998"
     CDS             89..406
                     /locus_tag="LK889_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK5097165.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="LK889_002999"
     CDS             complement(497..862)
                     /locus_tag="LK889_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097166.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1069..1551
                     /locus_tag="LK889_003000"
     CDS             1069..1551
                     /locus_tag="LK889_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097167.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1748..2638
                     /locus_tag="LK889_003001"
     CDS             1748..2638
                     /locus_tag="LK889_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBK5097168.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          919 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaagttttt ccccctaatt tttttagata
     1021 gaaaattgta tagtatgatg agagaaaaag tagtttgggg gaacgttaat gagaagaaaa
     1081 gcagtaatcg atcggcttta tttatttttt tggttttttc ctatcgtttt gctacctttt
     1141 acatgggtaa atgctgttgt acaaggtgcg gagatattag gcctgatcca aggaagcgga
     1201 tttgtggttt ctaggaattt tccagctttg attgttttct atttagtgat aagtgtaggt
     1261 ctcttattac caaaatctct gatcaatcac tacaattggc agctgcttta cttttcttgc
     1321 ttattagtat ttacatctac gttttctttg ctgcctcgct tgattatcgg ccctaatggt
     1381 ttgagcgaga gtggattgaa ggatttagct tattcctcca tgtatttctt gacgatcaaa
     1441 ccatggttct atttagtaat tgcctcattt attcttgcaa cattttttta cttctggaca
     1501 gaaatcagga taaaaagtga acgatctgta tctataaaaa ataactattg atggtggatc
     1561 atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt ctcatatgct ttttttatta
     1621 ttatgtaaag aatgcgaaaa acgtttacat gatttttaca taacttaact atatttttac
     1681 agcaaccata cgagggattt acagtcgact gataaacttt agttgtaaat aagaaaggag
     1741 caataaaatg aaaaaagtat tgtttactct aggtatgatg ggaattcttt tagccggatg
     1801 cggctcagga tcaggaaata cagatggctc tgccacacaa aataattcaa gtgactcaaa
     1861 tgaacaagta aaaatcgttg cggtaggttc gacagcatta cagccgttag tcgatgcagc
     1921 acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag
     1981 cggaactggt ttgagccaag tcgaagctgg agcagttact atcggaaact ctgatgtatt
     2041 tgcagaagaa agagacggag ttgatgcttc aaaattagtg gatcataaag tagcagtggt
     2101 cgggatggca ccaatcgtca ataaagatac agatgtcaaa gatatcacca aacaggaatt
     2161 gattgatatc tttacaggaa aaatcactaa ctggaaggaa gttggcggga aagatcaaaa
     2221 aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc gcgacattcg aaaaatgggg
     2281 actggatggt gctacccctg tgcagtccca agaacaagat tcatcaggaa cagttcggca
     2341 gctggtaagt caaacaccag gagcaatcag ctatttagct ttctcatatc tcgatgattc
     2401 cacacaagca ctaagtatcg acggtgtaga accaaaagaa gagaatgtag cagataacag
     2461 ctggggaatc tggtcatatg aacatatgta cacgaacgga aaaccttccc ctgaagtcca
     2521 aaaattctta gactacatga tgacagaaga aatccaagaa ggacctgtca aagaattggg
     2581 ctatttacca atcacaatga tggaagtaga acgtgatcac gaaggaaata tcaaataact
     2641 agcagataaa catgccacgg tagtcgttac tattaaggaa aaatcaacta tgtttcccca
     2701 catatacatt tttcctttaa ttgtgaaaaa tagttcctca tattttgccg ttttttgagc
     2761 taataccgag ggtcagctga ttgaacaccg tttattcgaa aaaaagagct acgacataga
     2821 actgtcgtaa ctttttttgg ctcttcgtca aatcgtgttg gtgaagacaa atttgaataa
     2881 aacttagacc gatatgcggc gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc
     2941 agaccttgtt gta
//
LOCUS       DAFBQW010000147         2731 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFBQW010000147 DAFBQW010000000
VERSION     DAFBQW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.153"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            179..775
                     /locus_tag="LK889_003002"
     CDS             179..775
                     /locus_tag="LK889_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5097169.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="LK889_003003"
     CDS             899..2698
                     /locus_tag="LK889_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK5097170.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          776 a          468 c          516 g          971 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac a
//
LOCUS       DAFBQW010000148         2714 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFBQW010000148 DAFBQW010000000
VERSION     DAFBQW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.154"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..929)
                     /locus_tag="LK889_003004"
     rRNA            complement(<1..929)
                     /locus_tag="LK889_003004"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1602..1958)
                     /locus_tag="LK889_003005"
     CDS             complement(1602..1958)
                     /locus_tag="LK889_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBK5097171.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(1972..2382)
                     /gene="spx"
                     /locus_tag="LK889_003006"
     CDS             complement(1972..2382)
                     /gene="spx"
                     /locus_tag="LK889_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK5097172.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          742 a          615 c          458 g          899 t
ORIGIN      
        1 gtttcaacct tgcggtcgta ctccccaggc ggagtgctta atgcgttagc tgcagcactg
       61 aagggcggaa accctccaac acttagcact catcgtttac ggcgtggact accagggtat
      121 ctaatcctgt ttgctcccca cgctttcgag cctcagcgtc agttacagac cagagagccg
      181 ccttcgccac tggtgttcct ccatatatct acgcatttca ccgctacaca tggaattcca
      241 ctctcctctt ctgcactcaa gtctcccagt ttccaatgac cctccccggt tgagccgggg
      301 gctttcacat cagacttaag aaaccgcctg cgctcgcttt acgcccaata aatccggaca
      361 acgcttgcca cctacgtatt accgcggctg ctggcacgta gttagccgtg gctttctggt
      421 tagataccgt caagggatga acagttactc tcatccttgt tcttctctaa caacagagtt
      481 ttacgatccg aaaaccttct tcactcacgc ggcgttgctc ggtcagactt tcgtccattg
      541 ccgaagattc cctactgctg cctcccgtag gagtttgggc cgtgtctcag tcccaatgtg
      601 gccgatcacc ctctcaggtc ggctatgcat cgtggccttg gtgagccgtt acctcaccaa
      661 ctagctaatg caccgcgggt ccatccatca gcgacacccg aaagcgcctt tcaaatcaaa
      721 accatgcggt ttcgattgtt atacggtatt agcacctgtt tccaagtgtt atccccttct
      781 gatgggcagg ttacccacgt gttactcacc cgttcgccac tcctcttttt ccggtggagc
      841 aagctccggt ggaaaaagaa gcgttcgact tgcatgtatt aggcacgccg ccagcgttcg
      901 tcctgagcca ggatcaaact ctcataaaaa gttcgaacaa tcttgcgatt gttaagctca
      961 atttgtttgc tagcatattg cttgcttgtt aaaaaatgtt tgttgtcttg aattgaatca
     1021 agacgcccta cacatttggt ttgtcttact ttgttcagtt ttcaaaggtc tacttcatca
     1081 ggagtgttcc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
     1141 aagtaattgt caactctttt ttaaaagttt ttttgatttc gtttttcgaa atcttacagc
     1201 aactcattta tcataactta tcatttgtta tttgtcaagg acttttttga agtttttttc
     1261 gatagaatca agcttcacaa gtggttcttt tttaacaact cagttatctt atcatgtcat
     1321 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
     1381 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
     1441 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
     1501 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
     1561 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
     1621 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
     1681 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
     1741 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
     1801 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
     1861 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
     1921 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
     1981 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
     2041 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
     2101 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
     2161 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
     2221 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     2281 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     2341 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcctttttaa
     2401 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataaaatata
     2461 aaaaaaatca aaataactga ttatttacgt ttgaataatg agaatttttc tatatattct
     2521 ctattttgat aatagtattc tcattttcaa aataaataag ggaggtgtga caaaattctt
     2581 gtcacacctc ccttttccga ataaactgtg ttccagaaat agcttcttcg aaaataagct
     2641 gaaatttcac aaaaatttga gaagcaattt tcgtacattc cttcttcttt cttgaaactg
     2701 accacttctg tcac
//
LOCUS       DAFBQW010000149         2636 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFBQW010000149 DAFBQW010000000
VERSION     DAFBQW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.155"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            106..1305
                     /locus_tag="LK889_003007"
     CDS             106..1305
                     /locus_tag="LK889_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK5097173.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1332..2600)
                     /locus_tag="LK889_003008"
     CDS             complement(1332..2600)
                     /locus_tag="LK889_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBK5097174.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT          789 a          573 c          468 g          806 t
ORIGIN      
        1 aaaacatctt tccatcggaa agatgttttt ttgctttttc tagtttaaat aacagctttt
       61 ttcatctatt ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga
      121 gatctgatct ttttagttct ctatcttatt ggggtattct ctatcgttta ttggcgaaaa
      181 cagaagaagg atccttcctt gttcttttac ggcagtacgt ggacatttac ggtaatctta
      241 acttattttg tcgtgttgga aattttttat tcgacctttt attttttgat tccattgttg
      301 ttctttagta tctttgcttt ttcttatttc acagaaaaac gaaggctact gaatggtctt
      361 ttattcaatg ttttactcat cagtttcggc atctacttgt ttgtcttgct gtatgaaacg
      421 caaaatattt ttcttggcgg actgatcgcc ttgatcacga ttccattgct acttgtcctt
      481 ttatttggta tttatggatt gattgttttt cttttttgga acggggtcac agttttacgt
      541 agagaatcgc attcattagc taatctttta acgttgatac tagccatttt tcttacactg
      601 tttcttgttt ttgacttttt cttgttaaaa tatttgccgc aatggataaa tgctttgttt
      661 ttctgcgttc ccctgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc
      721 tcattcttgt atcagttcaa ccggcctcga tacaatcaag acttcatcgt tgtgttaggc
      781 gctggattga tcaatggaga aacagtttct cctcttctag ccaagcgaat caataaggca
      841 attgcttttt atcgggctca aagccgtgct acattaaatc cgcccatctt attgatgtct
      901 ggcgggcaag gagcagacga aaaagtacca gaggccattg cgatgaaaca atatgccatg
      961 gaacaaggaa tccctgaaag agacattctt gtcgaaacca attcgacaac cactttggaa
     1021 aacatgctct attcaaagga aatcatggat cagcaaatga aagggccata ccgcgcgatc
     1081 tttagttcca acaattacca catttttaga gccggtcttt atgcgcggca agccaaattg
     1141 aaagcaaatg gtattggtgc aaaaacagct ttctactact tgcctaatgc gtttttaaga
     1201 gaatacctcg ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt
     1261 ttcgcaggct ccgcattctt atcgctgatt actttacttt tctaaaaaaa caaggtgtga
     1321 caaaacatat gtcacacctt gtttttttct ttcattctct taactattgg caaaaagagt
     1381 atcccttttt tgaccgctac ttgctgagca gaataaatac cgctgttgcc tgaacacata
     1441 aagctgatca agcagatcat aaagaagtat actgccgctt gtgaaccgaa caattcgatc
     1501 cccatcacaa agcaggcaat cggtgtattt gtcgcaccag aaaatactcc gataaagccc
     1561 aatcctgcaa gaaaaggaac agaaacatgg agcaaaggcg ctagtgctgc accaagcgtt
     1621 gcaccaattt caaacaatgg tgtcacttct cccccttgat atcccgcgcc taaagacaaa
     1681 actgtgaaga ataacttccc aataaaatca tagacatgtg cctgtccatt aaatgattct
     1741 tgaagtaacg gaagactcaa tccgagataa cgttgtgttt gcaaaatcaa tgctgctgcc
     1801 acaactacag ctccacctat cgtatttcga caaacaggat gaggaatcca ttgtgcatat
     1861 cgcgctttta aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc
     1921 ccagcaacaa aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg
     1981 taatgtagat gagaaacacc atagctttct gtcatgaaat ttgcaaacag tgcagcaaag
     2041 aagctaggga agatcgcttc tgttcggact ttcccgattg ccaacacttc taatccaaat
     2101 aatgttccag ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata
     2161 atcaagattt cccgttctga tttatccaat cggaacaaac gtgccgtatt atctgctaac
     2221 gctcccccca tttggactgc tgttccttca cggccaacag atcccccaaa taaatgggta
     2281 gttatggttc caaacagtgt aagtggaata agccgcaacg ggattttttc ttctccacca
     2341 tttccttgat cgatcactaa gttgtttcca cgactagaaa gtccgccgtg atacgcatag
     2401 agataagcaa aaagcgcacc actaactggt agtagacaaa ggagccaagg atgtgatagt
     2461 cggatatctg tcacaactgt caggctattt aagaaaaaag cagacaagct ccccatgaat
     2521 ataccgagaa tcgctgtgat caccagccac ttcagcatat acactaaaat gcgtactgga
     2581 ctcggtaatt gtttttccat aaaaaattcc tcctcaataa aaaaacgcct actaaa
//
LOCUS       DAFBQW010000150         2624 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFBQW010000150 DAFBQW010000000
VERSION     DAFBQW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.156"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            5..1150
                     /locus_tag="LK889_003009"
     CDS             5..1150
                     /locus_tag="LK889_003009"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBK5097175.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="LK889_003010"
     CDS             1321..1779
                     /locus_tag="LK889_003010"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK5097176.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="LK889_003011"
     CDS             1938..2408
                     /locus_tag="LK889_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBK5097177.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="LK889_003012"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="LK889_003012"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          860 a          503 c          494 g          767 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tgga
//
LOCUS       DAFBQW010000151         2474 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFBQW010000151 DAFBQW010000000
VERSION     DAFBQW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.157"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            8..81
                     /locus_tag="LK889_003013"
     tRNA            8..81
                     /locus_tag="LK889_003013"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="LK889_003014"
     tRNA            101..173
                     /locus_tag="LK889_003014"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="LK889_003015"
     tRNA            201..274
                     /locus_tag="LK889_003015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="LK889_003016"
     tRNA            288..361
                     /locus_tag="LK889_003016"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="LK889_003017"
     tRNA            385..474
                     /locus_tag="LK889_003017"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="LK889_003018"
     tRNA            487..560
                     /locus_tag="LK889_003018"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..651
                     /locus_tag="LK889_003019"
     tRNA            579..651
                     /locus_tag="LK889_003019"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            655..727
                     /locus_tag="LK889_003020"
     tRNA            655..727
                     /locus_tag="LK889_003020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Phe,seq:gaa)
     gene            732..802
                     /locus_tag="LK889_003021"
     tRNA            732..802
                     /locus_tag="LK889_003021"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Gly,seq:tcc)
     gene            815..888
                     /locus_tag="LK889_003022"
     tRNA            815..888
                     /locus_tag="LK889_003022"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:Ile,seq:gat)
     gene            900..971
                     /locus_tag="LK889_003023"
     tRNA            900..971
                     /locus_tag="LK889_003023"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:933..935,aa:Glu,seq:ttc)
     regulatory      1160..1239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1237..2430
                     /locus_tag="LK889_003024"
     CDS             1237..2430
                     /locus_tag="LK889_003024"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK5097178.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
BASE COUNT          674 a          477 c          619 g          704 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacattgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gtttggctcg
      661 gtagctcagt tggtagagca atggattgaa gctccatgtg tcggcagttc gattctgtcc
      721 cgcgccatta tgcgggtgta gtttagtggt aaaaccacag ccttccaagc tgttgtcgcg
      781 agttcgattc tcgtcacccg cttttagttt ttatgggcct atagctcagc tggttagagc
      841 gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt tattttattg
      901 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      961 cgtacgggtc atggaagata ctttttgagt atctttttct ttttatctga aagaatgatt
     1021 ttaaaaagct tagcagagtt atctttatgg atgattgtgc taagcttttt tggacagttc
     1081 taatgtaaaa tatttttata ttttttaagc aaaaaaatga aaaaatacga gcagaaaact
     1141 gttttatctg ctatacttgg ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg
     1201 ccttgtaacc gaatttaact agggggaatc attataatgg tagaaagaca cttatttaca
     1261 tcagaatctg tttctgaagg acatccagat aaaattgctg accaaatcag tgatgcaatc
     1321 ttggatgcaa ttttaaaaca agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca
     1381 actggtcttg ttttagtatt tggagaaata tctacgacgg catatgtaga tattcaaaaa
     1441 gtcgtacggg aaacaataaa agaaattggt tatacacgtg caaaattcgg atttgatgga
     1501 gatacagcag ctgtattggt tgcgattgat gaacaatctc ctgatattgc tcaaggagtt
     1561 gatgaagcac ttgagatccg ggatgaagat aaaaaagacg tattagatga gattggtgct
     1621 ggtgaccaag gtttaatgtt tgggtttgct gtagatgaaa caccagaatt gatgccttta
     1681 ccaatcgctt tgagtcatcg tctggtacga cgcttggcag atttgcgtaa atcaaatgaa
     1741 ttaacgtatt tacgtccaga tgcaaaatct caagtaacgg ttgaatatga tgatcaagga
     1801 caaccggaac gcgtagatac aatcgttatt tcaacacagc atgatgatgc agtagataat
     1861 gaaacaattc gtcatgatgt catcgaaaaa gtagtgaagg aagttattcc agctgaatta
     1921 ttagatgatc aaacgaaata ttatatcaat ccgactggcc gatttgtcat tggtggtcct
     1981 caaggggatg ccggattaac aggaagaaaa atcattgttg atacgtatgg cggttatgct
     2041 cgtcatggtg gcggtgcttt ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc
     2101 tatgctgcac gttatattgc taaaaacatt gttgcagcag ggcttgctcg caaagcagaa
     2161 gtacagctag cttatgcgat tggtgttgct caacctgttt cgatctcaat caatactttc
     2221 ggtactggaa cggttccgga agaagaactg attgctgcag tgagagaaaa cttcgatctt
     2281 cggccagcag gaatcatcga gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct
     2341 gcttacggtc attttggtcg tacagatgta gatttgcctt gggaacatac ggacaaagta
     2401 gatgcgttga aagcaagttt agcaaaataa gttatattca gctggaacga tcgccaagtg
     2461 gtggttgttc cagc
//
LOCUS       DAFBQW010000152         2397 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFBQW010000152 DAFBQW010000000
VERSION     DAFBQW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.158"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            206..2068
                     /locus_tag="LK889_003025"
     CDS             206..2068
                     /locus_tag="LK889_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5097179.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="LK889_003026"
     CDS             2068..>2397
                     /locus_tag="LK889_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5097180.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAFBQW010000153         2386 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFBQW010000153 DAFBQW010000000
VERSION     DAFBQW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.159"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(493..1785)
                     /locus_tag="LK889_003027"
     CDS             complement(493..1785)
                     /locus_tag="LK889_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5097181.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="LK889_003028"
     CDS             complement(2059..2319)
                     /locus_tag="LK889_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097182.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          786 a          448 c          360 g          792 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaa
//
LOCUS       DAFBQW010000154         2350 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFBQW010000154 DAFBQW010000000
VERSION     DAFBQW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.160"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..543
                     /locus_tag="LK889_003029"
     CDS             <1..543
                     /locus_tag="LK889_003029"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK5097183.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1016..1588)
                     /locus_tag="LK889_003030"
     CDS             complement(1016..1588)
                     /locus_tag="LK889_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5097184.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1604..2209)
                     /locus_tag="LK889_003031"
     CDS             complement(1604..2209)
                     /locus_tag="LK889_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBK5097185.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          468 c          359 g          783 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
      601 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
      661 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
      721 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
      781 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
      841 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
      901 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
      961 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1021 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1081 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1141 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1201 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1261 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     1321 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     1381 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     1441 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     1501 tctctacgcc gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     1561 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     1621 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     1681 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     1741 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     1801 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     1861 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     1921 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     1981 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2041 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2101 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2161 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2221 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     2281 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
     2341 cacagtctag
//
LOCUS       DAFBQW010000155         2164 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFBQW010000155 DAFBQW010000000
VERSION     DAFBQW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.162"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..111
                     /locus_tag="LK889_003032"
     CDS             <1..111
                     /locus_tag="LK889_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5097186.1"
                     /translation="YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            325..1020
                     /gene="vanR"
                     /locus_tag="LK889_003033"
     CDS             325..1020
                     /gene="vanR"
                     /locus_tag="LK889_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK5097187.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            998..2152
                     /locus_tag="LK889_003034"
     CDS             998..2152
                     /locus_tag="LK889_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK5097188.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          730 a          400 c          461 g          573 t
ORIGIN      
        1 tatgcggtaa agctatataa agaaggaaat atgactgtaa atcaaatttg tgaaattact
       61 aatgtatcta gggcttcatt atacaggaaa ttatcagaag tgaataatta gccattctgt
      121 attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa ctataaaata ttaacagcct
      181 cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca tcatcttaag aaattcttag
      241 tcatttatta tgtaaatgct tataaattcg gccctataat ctgataaatt attaagggca
      301 aacttatgtg aaagggtgat aactatgagc gataaaatac ttattgtgga tgatgaacat
      361 gaaattgccg atttggttga attatactta aaaaacgaga attatacggt tttcaaatac
      421 tataccgcca aagaagcatt ggaatgtata gacaagtctg agattgacct tgccatattg
      481 gacatcatgc ttcccggcac aagcggcctt actatctgtc aaaaaataag ggacaagcac
      541 acctatccga ttatcatgct gaccgggaaa gatacagagg tagataaaat tacagggtta
      601 acaatcggcg cggatgatta tataacgaag ccctttcgcc cactggagtt aattgctcgg
      661 gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag taaaggagca gaacgaaaat
      721 gttatcgtcc actccggcct tgtcattaat gttaacaccc atgagtgtta tctgaacgag
      781 aagcagttat cccttactcc caccgagttt tcaatactgc gaatcctctg tgaaaacaag
      841 gggaatgtgg ttagctccga gctgctattt catgagatat ggggcgacga atatttcagc
      901 aagagcaaca acaccatcac cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc
      961 attgataatc cgaaatatat aaaaacggta tggggggttg gttataaaat tgaaaaataa
     1021 aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt
     1081 ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg
     1141 gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata
     1201 tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat
     1261 tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac
     1321 cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga
     1381 tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc
     1441 aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac
     1501 gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt
     1561 agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct
     1621 aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac
     1681 gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc
     1741 cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc
     1801 tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga
     1861 taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa
     1921 gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct
     1981 ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga
     2041 aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt
     2101 tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta
     2161 tata
//
LOCUS       DAFBQW010000156         2109 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFBQW010000156 DAFBQW010000000
VERSION     DAFBQW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.163"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1369
                     /locus_tag="LK889_003035"
     rRNA            <1..1369
                     /locus_tag="LK889_003035"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1464..1579
                     /gene="rrf"
                     /locus_tag="LK889_003036"
     rRNA            1464..1579
                     /gene="rrf"
                     /locus_tag="LK889_003036"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1593..1665
                     /locus_tag="LK889_003037"
     tRNA            1593..1665
                     /locus_tag="LK889_003037"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1626..1628,aa:Val,seq:tac)
     gene            1669..1741
                     /locus_tag="LK889_003038"
     tRNA            1669..1741
                     /locus_tag="LK889_003038"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Lys,seq:ttt)
     gene            1749..1830
                     /locus_tag="LK889_003039"
     tRNA            1749..1830
                     /locus_tag="LK889_003039"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1783..1785,aa:Leu,seq:tag)
     gene            1848..1920
                     /locus_tag="LK889_003040"
     tRNA            1848..1920
                     /locus_tag="LK889_003040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1881..1883,aa:Thr,seq:tgt)
     gene            1932..2003
                     /locus_tag="LK889_003041"
     tRNA            1932..2003
                     /locus_tag="LK889_003041"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1964..1966,aa:Gly,seq:gcc)
     gene            2020..2105
                     /locus_tag="LK889_003042"
     tRNA            2020..2105
                     /locus_tag="LK889_003042"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2054..2056,aa:Leu,seq:taa)
BASE COUNT          552 a          466 c          621 g          470 t
ORIGIN      
        1 tcttaaggac aagctgtgat ggggagggaa ataatagtac cgaagttcct gatgtcacac
       61 tgccgagaaa agcttctagt gagaaaacag cggcccgtac cgcaaaccga cacaggtagt
      121 cgaggagaga atcctaaggt gagcgagaga actctcgtta aggaactcgg caaaatgacc
      181 ccgtaacttc gggagaaggg gtgctgatca tacgatcagc cgcagtgaat aggcccaagc
      241 gactgtttat caaaaacaca ggtctctgca aaatcgtaag atgaagtata ggggctgacg
      301 cctgcccggt gctggaaggt taagaggagt gcttagcgca agcgaaggta cgaattgaag
      361 ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt
      421 aagttccgac ccgcacgaaa ggcgtaacga tttgggcact gtctcaacga gagactcggt
      481 gaaattttag tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag accccatgga
      541 gctttactgt agtttgatat tgagtgtctg taccgcatgt acaggatagg taggagccgt
      601 agaaatcgga acgctagttt cgatggaggc gctggtggga tactacccct gcgttatggc
      661 cactctaacc cgcaccacta atcgtggtgg gagacagtgt cagatgggca gtttgactgg
      721 ggcggtcgcc tcctaaaagg taacggaggc gcccaaaggt tccctcagaa tggttggaaa
      781 tcattcgaag agtgtaaagg cagaagggag cttgactgcg agaccaacaa gtcgagcagg
      841 gacgaaagtc gggcttagtg atccggtggt tccgcatgga agggccatcg ctcaacggat
      901 aaaagctacc ctggggataa caggcttatc tcccccaaga gtccacatcg acggggaggt
      961 ttggcacctc gatgtcggct cgtcgcatcc tggggctgta gtcggtccca agggttgggc
     1021 tgttcgccca ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac agttcggtcc
     1081 ctatccgtcg cgggcgttgg aaatttgaga ggagctgtcc ttagtacgag aggaccggga
     1141 tggacttacc gctggtgtac cagttgttct gccaagggca ttgctgggta gctatgtagg
     1201 gaagggataa acgctgaaag catctaagtg tgaagcccac ctcaagatga gatttcccat
     1261 ttctttaaga aagtaagatc cctgagagat gatcaggtag ataggtcagg agtggaagta
     1321 cagtgatgta tggagcgggc tgatactaat cgatcgagga cttaaccaat aggttaaaag
     1381 aaaagaactc ggaaaaaaac ttatgtgact tcaaatccag ttttgagtga acaaaattta
     1441 ctcaataaaa gataacacca cagtgtggtg gcgatagcga gaaggataca cctgtaacca
     1501 tgccgaacac agaagttaag cttcttagcg ccgattgtag tgaggggttg ccccttgtga
     1561 gagtaggacg tcgccacgct taagttatcc atggaggttt agctcagctg ggagagcatc
     1621 tgccttacaa gcagagggtc agcggttcga tcccgttaac ctccatatga gccgttagct
     1681 cagttggtag agcatctgac ttttaatcag agggtcacag gttcgagccc tgtacggctc
     1741 atatttttgc gggtgtggcg gaattggcag acgcactaga tttaggatct agcgccttac
     1801 ggcgtggggg ttcaagtccc ttcacccgca taatttaagt tttacatgcc ggcttagctc
     1861 agttggtaga gcatctgatt tgtaatcaga gggtcgaggg ttcaagtcct ttagccggca
     1921 tctttaaaca cgcggaaata gctcagtggt agagcaccac cttgccaagg tgggggtcgc
     1981 gggttcgaac cccgttttcc gctttgccaa agcaatcgcg ccggggtggc ggaactggca
     2041 gacgcacagg acttaaaatc ctgcggtgag tgatcaccgt accggttcga ttccggtcct
     2101 cggcatttc
//
LOCUS       DAFBQW010000157         2017 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFBQW010000157 DAFBQW010000000
VERSION     DAFBQW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.164"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            99..569
                     /locus_tag="LK889_003043"
     CDS             99..569
                     /locus_tag="LK889_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBK5097189.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            791..1894
                     /locus_tag="LK889_003044"
     CDS             791..1894
                     /locus_tag="LK889_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK5097190.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          327 c          459 g          545 t
ORIGIN      
        1 aatctctgaa gaataaatca cgtgatcgtg acgataaaat acacttacgt caggtgataa
       61 aaaatacata agtagaaaac taagaggagg aacagaaaat gaaagaagtt caattgatca
      121 gaaaaagcga actatcagaa ggtggttgca atgcatgcgg cgtagtagaa gcaacaagct
      181 atacattaaa gcttggtgcc aacaaagcga taatctctga gttgacagta ggcggtcttg
      241 tggacagtct ggcgcttgct gaaggtttta ttggagaaga tatttatgag atgttttcag
      301 aaatccgtca gttaaaaaaa ggggaaaact gcatagaggt tcatcatgag agcccgaatg
      361 tgagattcaa acgtggtgat aatgagatga tcttcaataa tcatgtgtcc gatcacaccg
      421 agctatatgg aatcgtgaat caaatactaa ctgagctttt tggattggga ccttatgcgt
      481 tcaaggaaga aaacggtaat ccaaaattaa acgaagaatg gcaagaaacg attgaaatac
      541 aaagaaacaa cccgcactta tttcaatagg catccacagt atttcgtaaa agggactttg
      601 acacgacttt tgtcaaaatc ccatcgtttt ttatctattt gtgttctaaa catttctttc
      661 agagtagtaa caactggaat gactgaaatg taaacagatt atttgcctgt atcaatacgg
      721 aagagtaata taaagtgtgt aaacgctatc atattattca ttcaattttt tatagaggga
      781 aggcattgaa atggaaaaaa cgtatcaagc aggtacaaat gaaggaatag tggattttat
      841 caatatggaa gatttagaaa tagccgcttc acaagtgatt ccagctggtg gttatggtta
      901 catcagcagt ggtgctggcg acttatttac ttatcaagaa aatgaacggg cattcaatca
      961 tcagctgatt attcctcatg tattaagaga tgtagaatta cccgatacaa ctacacattt
     1021 tgacaaagaa acgctgactg caccaatcat catggcacca gttgctgctc atggcttagc
     1081 tcatgtgaag gcggagaaag cttctgcaaa aggtgtagca gattttggga cgatctatac
     1141 agcaagttct tacgcttctt gtacattaga agagataaga gaagcaggcg gggagaaagc
     1201 accgcaatgg tttcaatttt atatgagcaa agatgatggg atcaatttgg atattcttga
     1261 agtggccaag cgtaatggag caaaagcaat cgtattgaca gcggatgcaa cagttggcgg
     1321 aaaccgtgaa acagaccgca gaaatgggtt taccttccca ctgccaatgc cgatcgttca
     1381 ggcttatcaa tcaggagtag gccaaacgat ggatgctgtg tacaaatctt ccaaacagaa
     1441 gcttagtcca aaagatgtag aatttattgc tgctcattct gatttgccag tctatgtgaa
     1501 aggtgtccag tcagaagaag atgtctatcg ttcattagaa tctggtgctg gcggaatctg
     1561 ggtatctaat catggtggcc gtcaactaga tggtggtcca gcagcgtttg attcgttgca
     1621 atatgtggca gaagcagtag ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg
     1681 cggacagcat gttttcaaag caatcgcatc aggtgcagat cttgttgcta ttggcagacc
     1741 cgttatttat ggattgtctt taggaggaag tacaggagtc catcaagtgt ttgatttctt
     1801 taaaacagaa ttggaaatgg tcatgcaatt agctggaaca caaacagtag aagatatcaa
     1861 aaaaataaaa ttgcgtgaga atcgttttat ttaagaaaca aatgacgaaa aaaataggag
     1921 tgcggcagat gatgccgtac tcctattttt atagttcaag gatgtcacta atagaataat
     1981 ttgaaacgaa aaaataaaat tgaaatattt tttcata
//
LOCUS       DAFBQW010000158         2001 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFBQW010000158 DAFBQW010000000
VERSION     DAFBQW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.165"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..376
                     /locus_tag="LK889_003045"
     CDS             <1..376
                     /locus_tag="LK889_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK5097191.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="LK889_003046"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="LK889_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK5097192.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="LK889_003047"
     CDS             1857..>2001
                     /locus_tag="LK889_003047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097193.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DAFBQW010000159         1980 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFBQW010000159 DAFBQW010000000
VERSION     DAFBQW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.166"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(325..529)
                     /locus_tag="LK889_003048"
                     /pseudo
     CDS             complement(325..529)
                     /locus_tag="LK889_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(729..1745)
                     /locus_tag="LK889_003049"
     CDS             complement(729..1745)
                     /locus_tag="LK889_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK5097194.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          558 a          459 c          309 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaaa attttcctaa ttgtgacatg gctgtcacaa
      541 ttccaaatgc tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa ggagccgaaa
      601 gcgtgtcacg ctctcaactc cttttacgct ttattttgct tcatgcaaaa tattcgctta
      661 tcgtgaattt acgtcactaa acattgacgc aaatccactc tcactcatct gacttttctt
      721 attcggtatc acttctctgt cacatccaac cccaaaagcc acgtatccaa ttcttttttt
      781 aattcgtctg cagattcctt aaacgtctct cgtaaatgat tcgttaactc atcattttga
      841 ctttcttgct tcagcaaatt ccgatattta tattttgtat gtctagcaat tatcgaccaa
      901 ttagaaggct cagctatcaa accatcaata actattttat caataggttt tatatcaatc
      961 ggcaaaaaat gcgggcttgc aaaactatct aagctttcct gaaccatcgc atgccaatcc
     1021 gttgctttgc cacgtcttag ctgcatttct aaccgccacc acgtatcgat ttcttttggc
     1081 acgattactt tttttcgttc ctgctctagc tttttgttgt acagtcgcac ttggcgctca
     1141 ctggcacgac tgccccagta cgctgtttct agcttgccag aacggccgta gattggctta
     1201 aaagaaatcg ggtctacaat acgatactga gtaataaagt catccggcac atctacaata
     1261 tcgcaggcaa tatccgcccg ggaaaaatgc ggatctaaaa ataggtcatg aatgaatcct
     1321 ttcatggaac tagctaaaaa ctggcctatc ttattcggat taaaatcaat acgacctttt
     1381 ccttcatgaa atttaagtat ttcgatatac gcaatatctt ctccatattg atcgaataat
     1441 tgccagccgt ttatcccaac tgatttcaaa tatccctgat tttcgttcaa acgcattaat
     1501 tgctcgaaat cgacaaaaac aactccattt ttcgtccaat aagctatggt atcattcagt
     1561 tttccgacaa ttgtaattcg atcaatcgac caatttaatt ttaggggggt ggtcgtacct
     1621 tgcacactta accgcctgtt agaaatacgg ttaagttttt cgagctgatt tatcgttgtt
     1681 cttttttctg cccctcgacc ttcaattttt gattgaattt cactcaactg attttcattt
     1741 gccattttcg tcctccaaac gaaaaaagag acttttggaa agaatttcca gagttcacgt
     1801 aaccctaaaa attcatatcc aaaaatctct agcttgaaga aacctaaaaa tattcgtata
     1861 atgaacctga gttacggagc taggtgaagt agtttccgta aatagtcgag agtgttagcg
     1921 cactgctcgg cttttatttt gctctttttt ctcgttttct ttgattatag cactcctcct
//
LOCUS       DAFBQW010000160         1972 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFBQW010000160 DAFBQW010000000
VERSION     DAFBQW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.167"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(62..241)
                     /gene="rpmF"
                     /locus_tag="LK889_003050"
     CDS             complement(62..241)
                     /gene="rpmF"
                     /locus_tag="LK889_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK5097195.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(299..868)
                     /locus_tag="LK889_003051"
     CDS             complement(299..868)
                     /locus_tag="LK889_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBK5097196.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1054..1956
                     /locus_tag="LK889_003052"
     CDS             1054..1956
                     /locus_tag="LK889_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5097197.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
BASE COUNT          587 a          419 c          332 g          634 t
ORIGIN      
        1 aaaataaacc tcaaggctcc atggttagcc ttgaggtttt acattttcta attaaaatgt
       61 attaagcttc ttttgacatt acgtccttac catcatagta accacaattt gcacatacgt
      121 gatggctttt tttcatttca ccacagtttg gacattcgtt taaccctttg attgtcaatt
      181 tgtagtgtgt acgacgtttt gcttttttag cttttgatgt tcttctagct ggtactgcca
      241 ttttgctaca cctccttgta aagtcttgta tctaaattag acacaatata ttccaatctt
      301 acttgttatc gtcgtctttt gatttagaat caaaaagttc agatagtttt gcaagacgag
      361 gatcaatttt attctcggct tcttccattt ttctgcgctg gtattcttct tcggaaacaa
      421 cttcccaatc attgcctttt ggaagttctg ttgctgcttc ctcttctttt gtcaataccc
      481 gcattggaat cgccaataag atgttgtctt caatcgactc tgctaaattc aataatggac
      541 cttcaattag caaaatctca tctgcagaca tttgttcatc tctactttgg aattgttctt
      601 ctgtcatgaa tacttcgtct acagaaaaat ccatgggcag ttcaacaggt tctagtgaac
      661 gtgttgaagg aactgtcaaa gtcgtctgca cagtgtaatg aacgatatag cctttcttat
      721 caactgagac caaaccttcg acttccactg gcgcaacatc aagtattaaa ttgtctcgct
      781 tcatgagagc ggctttcaag tcgagcgtct catgaaaagt taacggtgtc tcctggtatt
      841 ttctcaattc caataaagac catttcattt ttcatcactc ctaagcaaca aaagcaatta
      901 tacagggcaa aaagcatgtt gtcaatgaaa ttttcttgac accttgcagt tcataaaaaa
      961 acagaaaaat ctttattaga aagcctctgt ttgctatcat cctcagaatc atttacaata
     1021 atcattattc cattaaagga gcggatgatc ttcatgaaca cgaaagatat cgaatactat
     1081 gtccaaatcg tcaaacaaaa aaattttacg aaagtggcta attatttccg tgtgagccaa
     1141 cctactatta catatgcatt acaacgtctt gaaaaagagt ttgacaccgt acttattaca
     1201 agagatcgat cgcatcacga attaatcgtc acgccttctg gaaagcagct gtttctccat
     1261 gcacaaacca ttctccaaga gatttctact gcaagagctg aaatcactca gttgcaaaag
     1321 aaaaagcttc gttttggcat gcctcctatt atcggcaatg aatactttcc taaactgtca
     1381 agttacttta tcaagcatga attgatgaat caaattgaga ttaccgatgg aggctctcgg
     1441 gacttatacg ggatgatccg acaagggagg atcgatttag ctgtattagg ctcaacacag
     1501 ccaatcgtgg atcaatattt agaaacagag ttacttttgg acaaacgttt catgatcgtc
     1561 gtgccaccta cacatcctct ttccaagcag gatactgtct ctttcaaaga tatagcagat
     1621 gaacagtttg tactattgaa cgaacattat gtccaccccg cagcattcac taagctttct
     1681 aaacaaacac atactcatcc aaaagttgtt tatcaaagca atgacttgac gattttaaag
     1741 agtatggtcc gagaaaatgt gggtatcggt tttttggcag agattgctgt ccatcctgaa
     1801 gatcatttag tttgcctccc tctttcagat gaagttcagc ctcgcttctt gatttcattg
     1861 gcaaaacgaa cacagcagat cgtcacgcct cttcaagaac aagtagtcca agttatccat
     1921 gaattcgtta cagcacaaaa aaacagctct tcgtaaaaga agagctgttt tt
//
LOCUS       DAFBQW010000161         1752 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFBQW010000161 DAFBQW010000000
VERSION     DAFBQW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.168"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(192..569)
                     /locus_tag="LK889_003053"
     CDS             complement(192..569)
                     /locus_tag="LK889_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097198.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(869..1750)
                     /gene="galU"
                     /locus_tag="LK889_003054"
     CDS             complement(869..1750)
                     /gene="galU"
                     /locus_tag="LK889_003054"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK5097199.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          503 a          381 c          254 g          614 t
ORIGIN      
        1 aaacattggt atgatttata tatatataaa taacataaaa aaaggaatgg cttatcccga
       61 tacaacaagc attcctttaa aaaagattct tttatataag tgaaaaaagc ttttttccac
      121 caattaaata aatctctcat ctcaatagtt tatccattat tttatctttt tttgcgtata
      181 cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta
      241 gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt catatatggg
      301 atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta
      361 cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa
      421 atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat
      481 agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc
      541 gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa
      601 aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat
      661 cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct
      721 attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa
      781 aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt
      841 tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact
      901 ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat
      961 ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg
     1021 catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc
     1081 ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc
     1141 cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta
     1201 ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt
     1261 cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca
     1321 tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa
     1381 acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat
     1441 gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt
     1501 ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt
     1561 caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg
     1621 cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca
     1681 tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt
     1741 tgaccttcac tt
//
LOCUS       DAFBQW010000162         1698 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFBQW010000162 DAFBQW010000000
VERSION     DAFBQW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.169"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..903
                     /locus_tag="LK889_003055"
     CDS             <1..903
                     /locus_tag="LK889_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK5097200.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..>1698
                     /locus_tag="LK889_003056"
     CDS             916..>1698
                     /locus_tag="LK889_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5097201.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFS"
BASE COUNT          564 a          248 c          389 g          497 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttca
//
LOCUS       DAFBQW010000163         1636 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFBQW010000163 DAFBQW010000000
VERSION     DAFBQW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.170"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(183..1502)
                     /locus_tag="LK889_003057"
     CDS             complement(183..1502)
                     /locus_tag="LK889_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBK5097202.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DAFBQW010000164         1583 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFBQW010000164 DAFBQW010000000
VERSION     DAFBQW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.171"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            292..1479
                     /locus_tag="LK889_003058"
     CDS             292..1479
                     /locus_tag="LK889_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5097203.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          315 g          505 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta ctt
//
LOCUS       DAFBQW010000165         1529 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFBQW010000165 DAFBQW010000000
VERSION     DAFBQW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.173"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..85)
                     /locus_tag="LK889_003059"
     CDS             complement(<1..85)
                     /locus_tag="LK889_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5097204.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            313..855
                     /locus_tag="LK889_003060"
     CDS             313..855
                     /locus_tag="LK889_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097205.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1011..1460
                     /locus_tag="LK889_003061"
     CDS             1011..1460
                     /locus_tag="LK889_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBK5097206.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
BASE COUNT          568 a          205 c          297 g          459 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa gttcttcgtg ttctcttaga
      181 cataaaaatt cctcctggta tgttttcttc tagtctacac accttaattt ttctgtctag
      241 ttaattgtag cctatccagt ttggaaaaat aaaaaattat atttcatcaa cgtttcataa
      301 aaggtgtgta gaatgttatt gaaagtagct ataaaatgct attgtgtaaa aaataaagga
      361 ggaataattt atgaaaaaat agtaccaaga ggagcatgcc tattacttgc attatgtttg
      421 agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca ataaaataaa agaaggacaa
      481 tccataagtt ctgaagagga aggcatgtca attaatgcaa atggcaaaaa tgaaactttt
      541 aagccatcag attatacatt ggaggcaaag aaagaatatg tttatgaata tttagggctt
      601 aaatttaaac tctcagacaa atttagaaat tatatagctg ataaaaagat agcgatgtta
      661 gatgatcaaa gtcctataga taaagaacta aagtatgcta ttttgacatt tgagaaaatg
      721 acagaagaac aaaagaatgc agttatagaa aagatgggcg atggatataa aaattggcaa
      781 aatgagcttg aaagaattgg gactattggt atatttgaaa aaaatacatc agaagaaaaa
      841 aatctaaaat tataaaatgt gatactcata ctaaaatagg agtttcaagt gatggaaaat
      901 acgattgcta tttaagcaca aatagcggag ctgaaagtaa tcttcttgat gaattaaaaa
      961 gaactgagat tcaaattata gataaaaaag aacgtcctaa aaatggtttt gtgctatcag
     1021 aaaaaactga tttagaaaat acagaagcat ttaacaaaga atcggttaaa gatttacgta
     1081 aactaccaac aaaggacata aatggcaagg attttacaag taaagatttt gagaagtatg
     1141 atcttactat ggtaaatgta tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg
     1201 atttggttga ggttcagaat gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta
     1261 cagatcctgt tgatgataac ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac
     1321 atgaaaagac aaaggcttca tatcctttct taatgccaga tggaatggca acactttttt
     1381 tgacaaaaat tataaggtgt tgtgaatgct gcggtgttat ggctccgatt gcccttgaca
     1441 atgagcatcc tcggatctga ttttctccgg cagaagcaca atcattcaat tacgctgcct
     1501 ctccagaggt atcagatccg cgctcatta
//
LOCUS       DAFBQW010000166         1492 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFBQW010000166 DAFBQW010000000
VERSION     DAFBQW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.174"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(56..850)
                     /locus_tag="LK889_003062"
                     /note="aph(3')-IIIa"
     CDS             complement(56..850)
                     /locus_tag="LK889_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBK5097207.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(943..1485)
                     /gene="sat4"
                     /locus_tag="LK889_003063"
     CDS             complement(943..1485)
                     /gene="sat4"
                     /locus_tag="LK889_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBK5097208.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
BASE COUNT          384 a          364 c          245 g          499 t
ORIGIN      
        1 attagatgtc taaaaaactt gtagttaaag ctttttagac atctaaatct aggtactaaa
       61 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
      121 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
      181 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
      241 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
      301 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
      361 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
      421 ccaattcggc taatcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
      481 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
      541 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
      601 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
      661 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
      721 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
      781 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
      841 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
      901 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
      961 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     1021 gattttgaaa ccacaattat gatagaattt acaagctata aggttattgt cctgggtttc
     1081 aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat tgataagcgc
     1141 gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta tataagcgta
     1201 ccggttccaa ttttttcgca gtttaacttt tccgacgcat ttatcgtctt ggtagtaaag
     1261 atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc
     1321 atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt
     1381 ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg
     1441 tttatcgata tctttcaggt gccctgcttt catttctgta atcactgttc cc
//
LOCUS       DAFBQW010000167         1487 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFBQW010000167 DAFBQW010000000
VERSION     DAFBQW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.175"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            357..1229
                     /locus_tag="LK889_003064"
     CDS             357..1229
                     /locus_tag="LK889_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5097209.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          474 a          252 c          318 g          443 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac tccctgagta aagatggaat
      121 tttctttcca aaatcaaata atatagatta atcaaatatt tgagaataac attttattta
      181 atcttttcat atatcaattg tatgaaaaat tattagaatt ttaaaaaatg ataaaaattc
      241 aaaaagaaaa aatagtagtt atttacttgt catgttacta aataaaaaaa taatttaaat
      301 atgatatata gtcatttatt tagtgatttc attctaaaat aaagggagaa atgtctatgc
      361 tatacggtgc aattgaagca ggcggcacaa aatttgtgtg tgccattggc gatgaagaaa
      421 tgacgatcaa ggaaagagtg agctttccga caacgacacc tgaggaaaca atgcctttag
      481 tcattgactt cttcaaacaa taccaagcag atttagcagg gattgggatt ggctcatttg
      541 ggcctatcga tatccatcgc gattccgcca cttatgggta cattacctcg accccgaaat
      601 tggcatggca aaattttgat ttcatcggta ccatgaaaaa agaatttccg atcccgattt
      661 cttggacaac agatgtcaat gcggctgcct atggtgagta tgtcttcggc agtggcaaag
      721 gattgagcag tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt gccttacaag
      781 aagggcgctt tatcgaaggg tttagtcatc cagaaatggg gcatatgctc gtcgtccctc
      841 atccaaaaga tgactttgct ggcagctgtc catttcatgg caactgttta gaagggatgg
      901 ccgctggacc cgccatcgaa aaccgcttag ggaaaaaagg acaagaggtt gcagaagatg
      961 atccttattg ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat acaaccttga
     1021 tgttttctcc agatgtgatc atttttggcg gtggtgtgat gaaacaacgc catatgctgc
     1081 aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca cctgagttaa
     1141 gcaaatatat cgtgacccca gcattagaag acaacgctgg aacgctcggt tgtttggcat
     1201 tggctcgaga agcggtctta cattcataaa cgaaaaaagg ctcttcgaca tttagtgttg
     1261 gtagagaaag atacaactga aaatttagtc ataaccagtt aggcagagtg cttaactggt
     1321 ttttgtttta cttggaagat caagccttga atgagataaa ttcttgctcg aaagttatgg
     1381 aaatttcgat agccgtaggc cacttgtatt gtgcaatagt tttttacaaa aaaaaggggg
     1441 tgcggatatt tttttggacc tttttaacaa atatatcttt gcttctt
//
LOCUS       DAFBQW010000168         1482 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFBQW010000168 DAFBQW010000000
VERSION     DAFBQW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.176"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(292..591)
                     /locus_tag="LK889_003065"
     CDS             complement(292..591)
                     /locus_tag="LK889_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097210.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(597..827)
                     /locus_tag="LK889_003066"
     CDS             complement(597..827)
                     /locus_tag="LK889_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097211.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1077..1277)
                     /locus_tag="LK889_003067"
     CDS             complement(1077..1277)
                     /locus_tag="LK889_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5097212.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          523 a          243 c          215 g          501 t
ORIGIN      
        1 aagagtttaa agcgttcccc aaaaaccact gccccataag tgtgttacgc tttaaactct
       61 tttatatttg aagccattaa aaagcatacc atatttttga aaaaaagtga gaaaaaaggc
      121 ttataattgg agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat
      181 aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat atctcctttc
      241 ataagtatgg tgataaaatc cgttccgggc taccttttta ggtagcctac tttaatcttt
      301 gtatctttct ggatcaacga aagtatactt tatatagtca taacgccgat gatcgcttcg
      361 agcgtctagc acatcagtca cgatatcaaa caaaaaatat acgtctttct tcattctagt
      421 tttcgcagca gggattttaa agtagttctt attagaatag tagagattaa ttaataagct
      481 atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat ctttgacaaa
      541 tctattcgaa ggatcaaatt taaaccataa ttgcgtctca ttaaaaagca taaccattac
      601 tccgatctat ttttaaactt agtctctacc tctaatatat atcgagtttt tattttgcct
      661 tcagagaata ctgtttcttt ttttgcagtt acaggttgtt tattttcgga aaaagctaat
      721 atagctaaaa ttgaaacatc catctggaat ttgtcttttt cgttgctttg ctcataaaaa
      781 tctgcatatt catcactaat attttttcta ataaattctt ccatcataaa gatcacctcg
      841 aaaagagtat acgaacaaac gttctttttg taaaggtgga atttattata ctaaataaat
      901 aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa atcgaattta ataaaacaca
      961 aacaaaaaga aatcctatta tgataacatt tcttataata acaaacacaa cagaagacgt
     1021 gtaatagtta gtcaggaacg tacaaataac ccctgtatcc tttgcggtac agggggttat
     1081 tttgtataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta
     1141 agtatattta ctccttgata aagtaaaata gatgcatgcg tatgtcttag tccatcaata
     1201 aacagtcgaa gagaaaatag ggaaaaattg attactaaag aaaaacttac taggtcgacg
     1261 gtgtagttga ttttcatact tcaatagatt cgaattaaac gatttcgatt ggcttatacc
     1321 agtcctatcc aacgaaagat atatcgttct taactctagc ggtataactt gaagctttac
     1381 gtaaagccaa tgggaatgaa ctggagatga ctttttatta tttttagtaa tagctgatgg
     1441 gttaatactt acaactttcc ttttcaaagt accatataaa aa
//
LOCUS       DAFBQW010000169         1474 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFBQW010000169 DAFBQW010000000
VERSION     DAFBQW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.177"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..1143)
                     /locus_tag="LK889_003068"
     CDS             complement(<1..1143)
                     /locus_tag="LK889_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK5097213.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLF"
BASE COUNT          433 a          301 c          227 g          513 t
ORIGIN      
        1 aaataaatcc agctgaagcg aatcggtgta cacaagcttg ctataggtta ttccaacatg
       61 ccttacttct tgtccttcat aatacttatc aaacaagaaa agacaataat tctgcaattc
      121 ctttgtatta tctgtgatag ggatcttcat ctggtgtgaa aaccctggtc tcgtttctaa
      181 gatcgacgtg ccaatgtgta aatggacaca acctgtttta cagttatgct gcctaatgcg
      241 tatcgctact tgttccgcca tttcttttac caccagctcg atttcatttc ttctagtata
      301 atctcgtggt aacacttgtg aatttccata tgatttttcc tttgtcggtt ctggtgggat
      361 tgctatatct gttcgatcga tcccattagc atggaagtac agctgtaagc caatcacacc
      421 tagtctattc ttaatcgtat aaggattcca gttagcaaga tctctaatgc tcataatccc
      481 catctgattg agtctttttt tcattcgaga gccaattccc caaaagtccg tcatttcagg
      541 aatattccat actttttcag gaacgttttc gtacgtccat tccgcaacaa atccttcatt
      601 atgctttgct tcgttgtcta aggctagttt agccaaaagg ggattatctc cgacacctac
      661 tgtagcaatc aatcctagct cttctttaat attctcctgg atcatttgag ccagcttctt
      721 tctccgttga cttcgtgttc cttcagtcgt aaaaagattc agtgatcggg tcacttttaa
      781 gattgattca tcgatcgagt aaatcagtag atcttcatca gccacatatc ttctgaaaat
      841 attatttacc tgcatatttc gcttgatata taggttcata cgtggtggaa caacgtgtag
      901 tgttttagga aatggttgtg gtaagtcacg tggtctactc acatttgtaa taccataccg
      961 cttttttgct tcaggagaag aagctaatat caatcctgaa ccagtattat cagctcgact
     1021 cataacaaca agttctgttg ttaatggatc taaatttctt tctatacatt cgacacttgc
     1081 gtaaaagctt ttcatatcga ttaaaaagta atcatttact ggctcttttg aataatccat
     1141 cataaataac actccattct ttcacattat acaaacatat gttcgatatt gtaaagaaga
     1201 aatagctaaa tacataaaaa aagaagcata ggtatgctat caccctgctt cttcttttga
     1261 tttgtatgat caatttattc tatcacttta ctaattaatt tgaaaatcta ataaaaactc
     1321 tcactctaaa agagtgagag actgcaaatt agagtacgga cttttttgga gttccgttct
     1381 ttaatattaa caaatgaata aaatccttac aacatttgag aataagttat cagtagacac
     1441 ttacaaaaaa ttctcatact atttcctcca tcat
//
LOCUS       DAFBQW010000170         1456 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFBQW010000170 DAFBQW010000000
VERSION     DAFBQW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.178"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            108..1040
                     /locus_tag="LK889_003069"
     CDS             108..1040
                     /locus_tag="LK889_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK5097214.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="LK889_003070"
     CDS             1112..>1456
                     /locus_tag="LK889_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097215.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAFBQW010000171         1424 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFBQW010000171 DAFBQW010000000
VERSION     DAFBQW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.179"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(150..1337)
                     /locus_tag="LK889_003071"
     CDS             complement(150..1337)
                     /locus_tag="LK889_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBK5097216.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAFBQW010000172         1422 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFBQW010000172 DAFBQW010000000
VERSION     DAFBQW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.180"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..106
                     /locus_tag="LK889_003072"
     CDS             <1..106
                     /locus_tag="LK889_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5097217.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="LK889_003073"
     CDS             complement(359..508)
                     /locus_tag="LK889_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097218.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="LK889_003074"
     CDS             970..1182
                     /locus_tag="LK889_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097219.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          523 a          213 c          237 g          449 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt tt
//
LOCUS       DAFBQW010000173         1421 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFBQW010000173 DAFBQW010000000
VERSION     DAFBQW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.181"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            96..1388
                     /locus_tag="LK889_003075"
     CDS             96..1388
                     /locus_tag="LK889_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5097220.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          462 a          291 c          257 g          411 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gcatatggct
     1321 ttcgtagctt ttataatttc aaattgcgca tcatgattcg tcatggcaac gcattgattt
     1381 tcaactaaaa aagatcaaga ggaactgatc cacttgatct c
//
LOCUS       DAFBQW010000174         1381 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFBQW010000174 DAFBQW010000000
VERSION     DAFBQW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.182"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            62..1357
                     /locus_tag="LK889_003076"
     CDS             62..1357
                     /locus_tag="LK889_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5097221.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          436 a          274 c          264 g          407 t
ORIGIN      
        1 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
       61 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      121 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      181 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      241 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      301 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      361 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      421 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      481 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      541 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      601 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      661 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      721 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      781 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      841 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      901 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
      961 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1021 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1081 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1141 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1201 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1261 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat
     1321 tatgattcgt catggcaaaa catttttaac caaataaaca gaacagaaag aggttttctt
     1381 c
//
LOCUS       DAFBQW010000175         1370 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFBQW010000175 DAFBQW010000000
VERSION     DAFBQW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.183"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            35..1336
                     /locus_tag="LK889_003077"
     CDS             35..1336
                     /locus_tag="LK889_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5097222.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          446 a          275 c          253 g          396 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
       61 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      121 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      181 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      241 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      301 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      361 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      421 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      481 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      541 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      601 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      661 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      721 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      781 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      841 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
      901 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
      961 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1021 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1081 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1141 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1201 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1261 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1321 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAFBQW010000176         1336 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFBQW010000176 DAFBQW010000000
VERSION     DAFBQW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.184"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            82..>1336
                     /locus_tag="LK889_003078"
     CDS             82..>1336
                     /locus_tag="LK889_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBK5097223.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMR"
BASE COUNT          455 a          259 c          255 g          367 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaa
//
LOCUS       DAFBQW010000177         1297 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFBQW010000177 DAFBQW010000000
VERSION     DAFBQW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.185"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            141..398
                     /locus_tag="LK889_003079"
     CDS             141..398
                     /locus_tag="LK889_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097224.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="LK889_003080"
     CDS             complement(630..1061)
                     /locus_tag="LK889_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBK5097225.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaaa
//
LOCUS       DAFBQW010000178         1264 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFBQW010000178 DAFBQW010000000
VERSION     DAFBQW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.186"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..678
                     /locus_tag="LK889_003081"
     CDS             <1..678
                     /locus_tag="LK889_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097226.1"
                     /translation="QESTDSTEQTIQTSDSSTIQSSDETVDSSTVDSSEEPKIAEDDQ
                     IVKQTIHVQKIISSEQLDNQGQKLEKQEGVNGAKFSVYDVSDILQKMDVKDLTTDQIE
                     SQLKDRVKKLSSDQLKLISNGETKTIDQQTGVFEFSVEVQANQKQAYYIVNESSPENI
                     SNSEDILLLTPVSDKNGEFLKDVWIYPKSEASQPKEEVKKIVSTGVKKNFFENCWDFV
                     THLFSRD"
     gene            691..951
                     /locus_tag="LK889_003082"
     CDS             691..951
                     /locus_tag="LK889_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097227.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            948..>1264
                     /locus_tag="LK889_003083"
     CDS             948..>1264
                     /locus_tag="LK889_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5097228.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQ"
BASE COUNT          465 a          207 c          224 g          368 t
ORIGIN      
        1 caagaaagca cggatagcac agaacaaaca atccaaacgt cagatagcag cacgattcaa
       61 agcagcgatg aaaccgtcga ttcgtcaaca gttgattctt cagaagaacc taaaattgct
      121 gaagacgatc aaatagtcaa acagaccatt cacgttcaaa aaattatttc ttccgaacaa
      181 ttagacaacc aggggcaaaa gcttgaaaaa caagaaggag taaatggcgc gaagttttct
      241 gtttatgatg tatcggacat tttacaaaag atggatgtta aggatctcac tactgaccaa
      301 attgaatccc aattaaaaga tcgagtgaaa aaactatcca gtgatcaact gaagctaatt
      361 tctaatggag aaacaaagac aattgatcaa caaacaggtg tctttgaatt ctcggtagaa
      421 gtacaggcaa atcagaaaca agcttattat attgtcaatg aatcaagtcc tgaaaacatt
      481 tctaatagcg aggatatttt gctattaaca cctgtttctg ataaaaatgg tgaattctta
      541 aaggatgtgt ggatttatcc taaatcagaa gcttcgcaac ctaaagaaga agtaaaaaaa
      601 atcgtatcta ctggcgtaaa gaaaaacttc tttgagaatt gttgggattt tgttactcac
      661 ttgttttcta gagattaagg aagaaataaa atgaaactga tcaaattatt attattcaat
      721 ttttcttgtt taaccgcagt ctttttgtac tgcggtttat tattttctaa actatctatt
      781 ttgccaagct accaactata cctaatgagg attttaaaca taccagctgc ttccatgccg
      841 ctagatatta tggaaaagct cattaggaat ccattattta ctacgatcaa tttgatcgtt
      901 attgtcggaa tttcactagt catttataaa aaagagggga tgaagggatg aattttaaac
      961 actcgtggac aagacggtta aaatatgttt ttcccataat gatgatgtta ggtacattgt
     1021 ttgggttaaa aacagccaat gtctttgctg aaaaagttat tgtcgatccg tcaaatccaa
     1081 tccagaacgt aaagaataat tttattattc caaaagatgt tcctaatgca aaaacaaata
     1141 ttactgtaaa cggagcgtca aaagtttaca aatatcatgt tgatgccaac aaaggtggtt
     1201 ttacagacga ctacgtagga ttagtggagg gaaacaaaaa tatacgctat ccttctttcc
     1261 aaaa
//
LOCUS       DAFBQW010000179         1258 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFBQW010000179 DAFBQW010000000
VERSION     DAFBQW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.187"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..672
                     /locus_tag="LK889_003084"
     CDS             <1..672
                     /locus_tag="LK889_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097229.1"
                     /translation="QESTDSTEQTIQTSDSSTIQSSDETVDSSTVDSSEEPKIAEDDQ
                     IVKQTIHVQKIISSEQLDNQGHEKQEGVNGAKFSVYDVSDILQKMDVKDLTTDQIESQ
                     LKDRVKKLSSDQLKLVSNGETKTIDQQTGVFEFSVEVQANQKQAYYIVNESSPENISN
                     SEDILLLTPVSEKNGEFLKDVWIYPKSEASQPKEEVKKIVSTGVKKNFFENCWDFVTH
                     LFSRD"
     gene            685..945
                     /locus_tag="LK889_003085"
     CDS             685..945
                     /locus_tag="LK889_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097230.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            942..>1258
                     /locus_tag="LK889_003086"
     CDS             942..>1258
                     /locus_tag="LK889_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5097231.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQ"
BASE COUNT          457 a          207 c          228 g          366 t
ORIGIN      
        1 caagaaagca cggatagcac agaacaaaca atccaaacgt cagatagcag cacgattcaa
       61 agcagcgatg aaaccgtcga ttcgtcaaca gtggattctt cagaagaacc gaaaattgct
      121 gaagacgatc aaatagtcaa acagaccatt cacgttcaaa agattatttc ttcagaacaa
      181 ttagacaacc aggggcatga aaaacaagaa ggagtaaatg gcgcgaagtt ttctgtttat
      241 gatgtatcgg acattttaca aaagatggat gttaaggatc tcactactga ccaaattgaa
      301 tcccaattaa aagatcgagt gaaaaaacta tccagtgatc aattgaagct agtttctaat
      361 ggagaaacaa agacaattga tcaacaaaca ggtgtctttg aattctcggt agaagtacag
      421 gcaaatcaga aacaagctta ttatattgtc aatgaatcaa gtcctgaaaa catttctaat
      481 agcgaggata ttttgctatt aacacctgtt tctgaaaaaa acggtgaatt cttaaaggat
      541 gtgtggattt atcctaaatc agaagcttcg caacctaaag aagaagtaaa aaaaatcgtt
      601 tctactggtg taaagaaaaa cttctttgag aattgttggg attttgttac tcacttgttt
      661 tctagagatt aaggaagaaa taagatgaaa ctgatcaaat tattattatt caatctttct
      721 tgtttaaccg cagtcttttt gtactgcggt ttattatttt ctcaattatc tattttgcca
      781 agctaccaac tatacctaat gaggatttta aacataccag ctgcttccat gccgctagat
      841 attatggaaa agctcattag gaatccatta tttactacga tcaatttgat cgttattgtc
      901 ggaatttcac tagtcattta taaaaaagag gggatgaagg gatgaatttt aaacactcgt
      961 ggaccagacg gttaaaatat gtttttccca taatgatgat gttaggtaca ttgtttgggt
     1021 taaaaacagc caatgtcttt gctgaaaaag ttattgtcga tccgtcaaat ccaatccaga
     1081 acgtaaagaa taattttatt attccaaaag atgttcctaa tgcaaaaaca aatattactg
     1141 taaacggagc gtccaaagtt tacaaatatc atgttgatgc caacaaaggt ggttttacag
     1201 acgactacgt aggattagtg gagggaaaca aaaatatacg ctatccttct ttccaaaa
//
LOCUS       DAFBQW010000180         1253 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFBQW010000180 DAFBQW010000000
VERSION     DAFBQW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.188"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            1..999
                     /locus_tag="LK889_003087"
                     /pseudo
     CDS             1..999
                     /locus_tag="LK889_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          442 a          222 c          258 g          331 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtcaaaa aaactgttgc cattccaatg agaaaaagca
     1141 tggaatgcgc tgcatcaggt atcgaggctt agaaaaaaat caggtgcata cgatgcttgt
     1201 ttttgccgaa atgagtctga aaaaattggt cacttagcag tgggagcata tgt
//
LOCUS       DAFBQW010000181         1236 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFBQW010000181 DAFBQW010000000
VERSION     DAFBQW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.189"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..104
                     /locus_tag="LK889_003088"
     CDS             <1..104
                     /locus_tag="LK889_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5097232.1"
                     /translation="GTVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..724
                     /locus_tag="LK889_003089"
     CDS             104..724
                     /locus_tag="LK889_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097233.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            728..1177
                     /locus_tag="LK889_003090"
     CDS             728..1177
                     /locus_tag="LK889_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097234.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          446 a          200 c          280 g          310 t
ORIGIN      
        1 ttggaactgt agaattcccg attgaagaag gagccttgat catcgcaacc gttgatgatc
       61 caaagcaaaa tgtgttcact cttgagaaag tcggggtgat ctaatggcta aaactaaacg
      121 aatgatggaa acggatgaaa aaacaggtgt acaacgccaa ttctttccaa tcacacatgc
      181 ttccgcagtt cttgggttag aagaaataat agcaggagaa gcaacagttt tatctgttaa
      241 tggaaaaatt ggagccgtcg tcattactaa agaagatttg ggattagaga atgttctcac
      301 agaattaccc tatgcaagtg aagaagatga cggcattatc acggcagaaa tgtatcaaaa
      361 aattttaaac agtggagaag gtgactacgt gttgccagtt gccactatcg accgtttggg
      421 tggcataaaa gttggtgaat tattgacgat tgatgagaca gggaaagtct ctgcagtcag
      481 acaatctgat gtcaattttt cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta
      541 tactgctgga gaaaacatca ctattgatga agatggaaca attagttcta caggtggtag
      601 tgggactggt ggtgtcaatc agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca
      661 agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa ttggggaggt
      721 atagagaatg acagatatcg taaaaataaa acaaagcaat gtacaggttt atccacaaac
      781 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
      841 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      901 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      961 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
     1021 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
     1081 tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt
     1141 agccaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt tcaattaaaa
     1201 gaagacggag ttgctaaata cttaaaaacg catgta
//
LOCUS       DAFBQW010000182         1233 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFBQW010000182 DAFBQW010000000
VERSION     DAFBQW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.190"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..104
                     /locus_tag="LK889_003091"
     CDS             <1..104
                     /locus_tag="LK889_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5097235.1"
                     /translation="ETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..721
                     /locus_tag="LK889_003092"
     CDS             104..721
                     /locus_tag="LK889_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097236.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            725..1174
                     /locus_tag="LK889_003093"
     CDS             725..1174
                     /locus_tag="LK889_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097237.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          451 a          178 c          272 g          332 t
ORIGIN      
        1 ttgaaacagt agaatttcca attgaagaag gagctttgat tattgcaact gttgatgatc
       61 caaaacagaa tgttttcact cttgagaaag ttggggtgat ttgatggtta aagtaaagag
      121 aatgatggag actgatgaat ctggtgttaa acgtcaattt atgccaatta cacatgtttc
      181 tgcagtttta ggcttagaaa aaatgatatc aggacaatca aaagttcttt cggttaatgg
      241 taaatatggt gcagtcattt taacgaaagc tgatttagga ttagagaatg caattactga
      301 actaccttat gcaagcgaga caagcgatgg tattcttact gctgaaatgt ttcaaaagat
      361 cgtaaatgga gaaggtggaa catatatttt gccgattgct actcctgaac aattaggagg
      421 tataaaagtt ggggaactct tagaagttac tgaagaaggt gttttatctg caactaagca
      481 aacagacttc aatttctctg aagaattaaa gggaaaatta gaatctctaa aaatattaaa
      541 agcaggtgca aatatatcaa ttgcagaaga tggaacaatt agttctacag gtggtagtgg
      601 gactggtggt gttaatcagt catatgtaga tcaaaaattc caagaagctg taaatcaagc
      661 agaaaattac acaaacgaaa gaattccaaa ttttactttt gaaaaaatcg gggaggtata
      721 gaaaatgaca gatatcgtaa aaataaaaca aagcggtgta caggtttatc cacaaactca
      781 ttggaatgct atagaaggca aaccaacaac ggtgaaggga gataaaggcg atcctggcca
      841 agcagcaaca attaccatag gaaccgtttc tagtggttca accgcttctg ttacgaatgt
      901 aggaacatca tctgcagcta gatttaattt tgttttacca aagggagata aaggtgatcc
      961 tggaataaat gccacaacta cagctgtagc tacaactaca gcaaatgggt tgatgtcgtc
     1021 cacagataaa actaagttgg atggaattgc agctggagca cagaaaaatc caggtaatgc
     1081 tacaactacg acagcaggtt taatgtcagc aaccgataaa gtgaaacttg atggattagc
     1141 gaatattaca tttgagaaag tggggacggt ttaatggctg atattgttca attaaaagaa
     1201 gacggagttg ctaaatactt aaaaacgcat gta
//
LOCUS       DAFBQW010000183         1193 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFBQW010000183 DAFBQW010000000
VERSION     DAFBQW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.191"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1076
                     /locus_tag="LK889_003094"
     CDS             <1..1076
                     /locus_tag="LK889_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5097238.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFBQW010000184         1183 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFBQW010000184 DAFBQW010000000
VERSION     DAFBQW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.192"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            84..>1183
                     /locus_tag="LK889_003095"
     CDS             84..>1183
                     /locus_tag="LK889_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBK5097239.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRL"
BASE COUNT          441 a          223 c          211 g          308 t
ORIGIN      
        1 gtgtaaaagt aaaaaggcca tataacagtc cttttacggt acaatgtttt taacgacaaa
       61 aacataccca ggaggacttt tacatgaccc aagtacattt tacactgaaa agcgaagaga
      121 ttcaaagcat tattgaatat tctgtaaagg atgacgtttc taaaaatatt ttaacaacgg
      181 tatttaatca actaatggaa aatcaacgaa cagaatatat tcaagcaaaa gaatatgaac
      241 gaacagaaaa ccgacaaagt caacgaaatg gctattatga gcgcagcttt acgacacgtg
      301 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
      361 ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc aatgttggaa atgtatgtat
      421 caggcgtttc aactcgtaaa gtatcaaaaa ttgtggaaga actttgtggt aaatccgtct
      481 ctaagtcctt cgtttctagc ttaacagaac agctagaacc tatggttaac gagtggcaga
      541 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      601 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      661 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      721 caacattttt tgaataccta aaagaacgcg gtttacaagg tacggaactc gttatttctg
      781 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgta agttggcaaa
      841 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      901 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg
      961 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
     1021 cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc
     1081 gactaaagag taccaatcta attgaacgac tgaatcaaga agtacgcaga agagaaaaga
     1141 ttattcgcat cttccccaat caaacatcag ccaatcgctt aat
//
LOCUS       DAFBQW010000185         1173 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFBQW010000185 DAFBQW010000000
VERSION     DAFBQW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.193"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            31..291
                     /locus_tag="LK889_003096"
     CDS             31..291
                     /locus_tag="LK889_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097240.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(653..892)
                     /locus_tag="LK889_003097"
     CDS             complement(653..892)
                     /locus_tag="LK889_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097241.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          416 a          163 c          218 g          376 t
ORIGIN      
        1 taatggaaaa acattaagga tggatgaaat atgaataacg aaactatcat tttaggaaaa
       61 aaatatattt gccaacctat tggcttgaaa caaaaagtgg ttggagaagt aatcagtaaa
      121 ttagaaaatt gtgttgtgat ctgcgtagac gaatatcatg acgtcgatcg caatgaaata
      181 ttagaaaaat gcggaaaagt agtggcaaaa tatactcacg tttatgagtt ggaaacaaca
      241 gaatatctat ataactatag caacaaatat gaacatactt tagtttcata aagatttaaa
      301 taaagtgcct tcaaaagcga gaagaccaag cttttgaagg cactttattt agttggcagt
      361 gattgttcat gatttttgat ttattcaaaa ggttcgccta gagaatctgt ctctgtaacg
      421 aggcctggtg gagaaactcg ataaaaacct atactgctag aactgtccta ttttcggata
      481 gacaaactag tagttttaaa aagatcatcc gatccaatca tttcaaaaaa tgcaaaactg
      541 atatcaggat catttagctc aggcttgtta tcgtaaagca tttgtaaagg ttgcatttag
      601 ttaggagtaa acgcgaaact atcatcatta tttgctgaag aagatgaaga agctaattca
      661 gagtttgttt gaaataaagg agacgtgctg acagtttctt taccctgttt tttgcagagg
      721 tagaaacttt tatatcggaa gtttctaccg gagaattttt ctcatttgta gaaattgttg
      781 aatcgatact attcgaaatt tgcgtatctg aaggtatgtt ctttgatgca gaagtcccac
      841 aagcagtaag taacaataaa tttgataaac tgataaacag aattcttttc attatgtaca
      901 cactccttat agtatcgttt agcatacacc tatgctcagt attaggcaaa atcaatgcaa
      961 aactatcaag tcttttatag tatctattag tcttagtaat acatatatgg agagggtgat
     1021 atcccagttc aaaccagata cattggttac catctgtatt aacgattatg cctctaaacg
     1081 ctttttttat gaaaaaattt ataagtgaaa acgtaggtat gatattatct agaggtaatt
     1141 tttttgggta tttaaatagt tgctaggagg ata
//
LOCUS       DAFBQW010000186         1128 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFBQW010000186 DAFBQW010000000
VERSION     DAFBQW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.194"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            4..963
                     /locus_tag="LK889_003098"
     CDS             4..963
                     /locus_tag="LK889_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK5097242.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          383 a          209 c          224 g          312 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgttt
//
LOCUS       DAFBQW010000187         1113 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFBQW010000187 DAFBQW010000000
VERSION     DAFBQW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.195"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..>1113
                     /locus_tag="LK889_003099"
     rRNA            <1..>1113
                     /locus_tag="LK889_003099"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          341 a          222 c          318 g          232 t
ORIGIN      
        1 tccgaatggg ggaacccaac atctttaata ggatgttacg attgtgtgaa tacatagcac
       61 attcgaggta gacgcagaga actgaaacat ctaagtacct gcaggaagag aaagaaaatt
      121 cgattccctg agtagcggcg agcgaaacgg gaaaagccca aaccagcaag cttgcttgtt
      181 ggggttgtag gactccaata tggtagttct ttcagatagt cgaatgactt ggaaaagtca
      241 gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga cacctaggag gatcctgagt
      301 acggcggaac acgagaaatt ccgtcggaat ccgggaggac catctcccaa ggctaaatac
      361 tccctagtga ccgatagtga accagtaccg tgagggaaag gtgaaaagca ccccggaagg
      421 ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt caaagcccgt taatgggtga
      481 tggcgtgcct tttgtagaat gaaccggcga gttacgattg catgcgaggt taagttgaag
      541 agacggagcc gcagcgaaag cgagtctgaa tagggcgttt gagtatgtag tcgtagaccc
      601 gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg taaaacgcac tggaggaccg
      661 aacccacgta cgttgaaaag tgcggggatg aggtgtgggt agcggagaaa ttccaaacga
      721 acttggagat agctggttct ctccgaaata gctttagggc tagcctcgga attgagaatg
      781 atggaggtag agcactgttt ggactagggg cccatctcgg gttaccgaat tcagataaac
      841 tccgaatgcc attcattcat atccgggagt cagactgtga gtgataagat ccatagtcga
      901 aagggaaaca gcccagacca ccagctaagg tcccaaaata tatgttaagt ggaaaaggat
      961 gtggggttgc acagacaact aggatgttgg cttagaagca gccaccattt aaagagtgcg
     1021 taatagctca ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa acatattacc
     1081 gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAFBQW010000188         1089 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFBQW010000188 DAFBQW010000000
VERSION     DAFBQW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.196"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..276
                     /locus_tag="LK889_003100"
     CDS             <1..276
                     /locus_tag="LK889_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5097243.1"
                     /translation="RNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQ
                     GGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            619..875
                     /locus_tag="LK889_003101"
                     /pseudo
     CDS             619..875
                     /locus_tag="LK889_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          376 a          164 c          198 g          351 t
ORIGIN      
        1 cgtaatccct tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc
       61 caagaaaaag ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa
      121 tatttgtcgc aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt
      181 catgctagaa ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt
      241 gaaaaaagag aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc
      301 taatgtgagc tatgccttct atataagact aagttctatt ataagtaacg aagtaattct
      361 cgttccttcc tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct
      421 ccagtaaatt ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt
      481 agttggctac ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat
      541 ttactacaag gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa
      601 aatcacatca ggaaatgcat gagattcttt aagaaaggca attatagggc tatgattacg
      661 gttcgtgtaa gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt
      721 actttatctg aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt
      781 caaacggctg aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg
      841 gaagaatcct taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga
      901 aattcagaag cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat
      961 tcgttgttac cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt
     1021 atttttacaa aaaaataata actggaatct agttaaaact cttgaagtga gagatttgac
     1081 tagattttt
//
LOCUS       DAFBQW010000189         1061 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFBQW010000189 DAFBQW010000000
VERSION     DAFBQW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.197"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(36..992)
                     /locus_tag="LK889_003102"
     CDS             complement(36..992)
                     /locus_tag="LK889_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097244.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DAFBQW010000190         1051 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFBQW010000190 DAFBQW010000000
VERSION     DAFBQW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.198"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LK889_003103"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LK889_003103"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK5097245.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAFBQW010000191         1003 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFBQW010000191 DAFBQW010000000
VERSION     DAFBQW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.199"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..467)
                     /locus_tag="LK889_003104"
     CDS             complement(<1..467)
                     /locus_tag="LK889_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5097246.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATIS"
     gene            complement(484..888)
                     /gene="tnpA"
                     /locus_tag="LK889_003105"
     CDS             complement(484..888)
                     /gene="tnpA"
                     /locus_tag="LK889_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBK5097247.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          302 a          168 c          164 g          369 t
ORIGIN      
        1 gatattgtag cggatttgat aatccctttt ggttgtctat gaagcttaat tcttactaat
       61 gacttcaatt taggaacttt gatgaattta ctatcaatca aagcaactgt accattttga
      121 ttattcgttg tataactctg aacaggattt ttcttacttt tgaaacgtgg aaatccaacg
      181 gatttatccc gaaaaaaatt cttgtatgct ttatctaaat taagttgggc attggctaaa
      241 gcaaggctat caatttcttt caaaaacgga aattctttct tgtatttagc tggtgtcgga
      301 aaagtcattt ttttagaaga atcattttta acttcttcat acgccttttt tcggtcatca
      361 agcattagat tgtagacctt acggacacaa ccaaaagatt tggcaaagaa gatttcttgt
      421 tcctctgttg gatagattct gaatttgtat gcttttagtt gttccataaa gttcacctac
      481 tttctattta tgattgtttc cttgattttg gatatacttc ttaatcacat caattggtgc
      541 gccaccagtt gtcagaagac aaaaactttt agaccagaac atttctttcc aaagaaattg
      601 tttgactctt ggaaagtcac gttttatcaa tcttgagctg gcacttttat aggcattgat
      661 gaattttgtc aattctgttt taggttgagc tttgaacata atatgaacgt ggtctttatc
      721 atgattccac tccactaaag taatgtgata cgattctgaa attctttcaa aagtagtttt
      781 ggcataatca gatatttcat catcaatcac ttgtctacga tatttcgtta ctaaaacaag
      841 gtggtaataa agaagaaata ctgaatgatt atttgtatct aattccatgt tatatcaact
      901 catttcttgt atagactgat tgtaacatgg aacaaaataa aaaggcaata cgccttagtt
      961 gtcggtcttc gagttaccaa cggtaaccct cataccgaac ggc
//
LOCUS       DAFBQW010000192         1002 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFBQW010000192 DAFBQW010000000
VERSION     DAFBQW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.200"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            52..307
                     /locus_tag="LK889_003106"
                     /pseudo
     CDS             52..307
                     /locus_tag="LK889_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            371..1002
                     /locus_tag="LK889_003107"
                     /pseudo
     CDS             371..1002
                     /locus_tag="LK889_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          344 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaaaa
      301 tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt cccattgtta
      361 ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga agccgtcaaa
      421 aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt caattgtgga
      481 taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata ttttgcctac
      541 acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg aagcggtgga
      601 atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg tcttaccaaa
      661 tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg cgttcaatga
      721 ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg aagccgagaa
      781 agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc actatgatta
      841 caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag caatgatgat
      901 tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt tccatgagct
      961 gaccgaagcc tttagagaca aggaatcaga cttatttttt ac
//
LOCUS       DAFBQW010000193          943 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFBQW010000193 DAFBQW010000000
VERSION     DAFBQW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.201"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..258
                     /locus_tag="LK889_003108"
     CDS             <1..258
                     /locus_tag="LK889_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097248.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="LK889_003109"
     CDS             260..397
                     /locus_tag="LK889_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK5097249.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            434..727
                     /locus_tag="LK889_003110"
     CDS             434..727
                     /locus_tag="LK889_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097250.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            724..>943
                     /locus_tag="LK889_003111"
     CDS             724..>943
                     /locus_tag="LK889_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK5097251.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          350 a          134 c          201 g          258 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccatta tgcattacag acgaagaagc
      361 agataaaatc attaataaag ataagagcgc atcttaattg atgcgttttt atttaaggta
      421 aaggagttgt cacatgatta atttagggga atggggaatg atagcaggat caataaccgc
      481 tatcgtttct ttgattttat tagtaattag accgattgct gcatctttct cgaaaattac
      541 tgagactctt tcaaaagtaa gccacaattt agatttgctg actaaagatt tagaatcgag
      601 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
      661 acatacagaa aaattggtgg aacacacgca acaaatcaaa actttgttta gggagagaag
      721 aaaatgaata ataaaacgtt cgaagtacta aaatggttcg cactggtaat tattcccgca
      781 ctagctactt tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac agatatctgt
      841 gttgtcatca ttactggttt tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga
      901 acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa aca
//
LOCUS       DAFBQW010000194          879 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFBQW010000194 DAFBQW010000000
VERSION     DAFBQW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.202"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..>879
                     /locus_tag="LK889_003112"
     CDS             <1..>879
                     /locus_tag="LK889_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5097252.1"
                     /translation="VLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPW
                     NLLDNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATY
                     EDGSPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSF
                     YNNLNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSN
                     VQTWFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDL
                     LAVYDRFELIDNLPKEV"
BASE COUNT          311 a          148 c          175 g          245 t
ORIGIN      
        1 gttttagaag gcgtaacaac caacaccaaa gtcaatattg actatggaaa aatcggtacg
       61 tataacggga aaaaagtcaa tatcaagcta gttctatcta atattcactt atactcggat
      121 acgttgccgt ggaatctact agataacaac tatacaaaaa ctcattttcg agacgatggc
      181 tataaaaata cgaatggtgc aatgagtaaa tctaaaaaaa gaacagtact ttggatctca
      241 gacaatcttt tctcaggaat tgtttaccat tcaacacaaa tgaatgtgca gctagtagcc
      301 acatatgagg acggtagccc cgttcaattt tcaggagata catttatcag ttttaactct
      361 ttgaaccctg caggaggaaa aagcacggat cttaaaggag aatatgccca ctatgacaaa
      421 atgaatacga cagattggta tgtcagaaaa gatacggttc tttcagaatt taaatctttc
      481 tacaacaatc tgaacgtggt aggtggccac ccaggaggaa gctctaagct gacacaagcg
      541 gataatgatt tcaataattt gcatgataaa ttaggagatc cgaagtttgg tcaaggtact
      601 gtgtccttta aaatatctga agcaaatccg acgtttgtga ttggttcttc taatgttcaa
      661 acctggttta ctttatcttc tgcgacaatc tttagtgttg ttcctgatca acctgaaaaa
      721 acaggggttg ataaaaatgg aaataacgtg aacgataaaa tgttgcaagt aggagacacg
      781 attcaatatc gaatcaaaca gaaggtgaat cgattaggag tggatttact agcggtttac
      841 gatcgatttg agttaatcga taatttacca aaagaagta
//
LOCUS       DAFBQW010000195          874 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFBQW010000195 DAFBQW010000000
VERSION     DAFBQW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.203"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            744..>874
                     /locus_tag="LK889_003113"
     CDS             744..>874
                     /locus_tag="LK889_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK5097253.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTD"
BASE COUNT          342 a          122 c          114 g          296 t
ORIGIN      
        1 aagaaggtat caagagtcac agtgaattta ttccgaaaag tgaaaagagc aaatataaaa
       61 tttaataaaa atatagaact aaataggaaa acgtagacgt aattttctac gttttccttt
      121 tcatatatat taaatattac ggaaaattat ataaactaat ttagtgtttt gtttgtaatt
      181 cctaattttg ctccactttt agataatttt taggtctatt taactcatca gacattctat
      241 gctcattttc tccaaatttg tttcaactct ttcgtactta attttgcgta tttgaaaatt
      301 cttcttttag agtactttta ttacgatact agtatgatat atatctatag caaacagtta
      361 aaatcttcct aaaataagcg aatagcattc ctaaaaccta taaaattttt agatgaagcg
      421 acaagtggac aattaataaa ctacttgaac atgaaattat ggatcacctt tcaaatttag
      481 aaaatggaat tcttatactt attgcacaac atttattgat tgcaaaatta tgtgacaaaa
      541 agaactagta gaagaaggaa taatggcttg ataataaagt actacctatt aaaggtagta
      601 ctttatttaa tttagtataa attaacatac taacccgtgg tgctagttaa ttttttccta
      661 gaggaaaatg tctaaacatg ttggtaaatc aacaaaaaaa cgcatatagt agtctcaaaa
      721 ctcccaagaa atgaggacta catatgcgtt catcaaaata tactgaacat tatacagata
      781 cttttataac attcaaggaa attatgagaa ctgtttctaa catataccac aattgtgtac
      841 cagataagat taaaaaccga agaaatactg atca
//
LOCUS       DAFBQW010000196          844 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFBQW010000196 DAFBQW010000000
VERSION     DAFBQW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.204"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(35..235)
                     /locus_tag="LK889_003114"
     CDS             complement(35..235)
                     /locus_tag="LK889_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5097254.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            631..780
                     /locus_tag="LK889_003115"
     CDS             631..780
                     /locus_tag="LK889_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097255.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          272 a          144 c          134 g          294 t
ORIGIN      
        1 ttctttcgaa aagacggtta aaagtcaatt tttattaagc tttaacaatg tttgttgctt
       61 gagggccacg ttgaccatct tcaacatcgt aagacactgc ttggccttct tctaaagttt
      121 tgaagccgtc accttggata gctgaaaaat gtgcaaatac atcatttcca tcttcaccag
      181 tgataaagcc aaaacctttg tctgcgttga accattttac tgtaccgtta ttcataaata
      241 aattcctcct gatacgtatc aatgtacgtt ttttcgttgc aataaatatt tgataggcaa
      301 aaggagtttt atctatgtag atatatcgct caaattacgt ttcaaaatag attactcatt
      361 taatatatca tgctttttct aataacacaa ggataatgca atgaaatata aaattcattg
      421 tcttctattg tcttcgaatc gaagaaaaag ttgaataacc gcgattgatt cactttttgt
      481 tttcttgttt atttgctttt atctgcaact tttgacttta aaatttctta actatgaaaa
      541 cctttcaatt ggtggtatac ttataaatgg aatttcgtta tcaacgcgtt cgttgccttt
      601 cagcaagtat ttaaagaaag gagaatggct atgacattct ataacaaaga tatacaatat
      661 cgagtaacct tggatacaga tttgaatcta tttgtcgttt ttgacaaaga aaataccaat
      721 catgtagcga caggtgttac gatcgaacaa gcagtgcaag aattaaaaaa atctgcataa
      781 ggagtggaaa ccctcatatg gacgattctc gttcttctct ttaaacgcat ctactggtag
      841 ataa
//
LOCUS       DAFBQW010000197          790 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFBQW010000197 DAFBQW010000000
VERSION     DAFBQW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.205"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..782
                     /locus_tag="LK889_003116"
     CDS             <1..782
                     /locus_tag="LK889_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5097256.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc
//
LOCUS       DAFBQW010000198          763 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFBQW010000198 DAFBQW010000000
VERSION     DAFBQW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.206"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(237..716)
                     /locus_tag="LK889_003117"
     CDS             complement(237..716)
                     /locus_tag="LK889_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097257.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          260 a          102 c           85 g          316 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa catctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aac
//
LOCUS       DAFBQW010000199          735 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFBQW010000199 DAFBQW010000000
VERSION     DAFBQW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.207"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            220..>735
                     /locus_tag="LK889_003118"
     CDS             220..>735
                     /locus_tag="LK889_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK5097258.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT          255 a          114 c          163 g          203 t
ORIGIN      
        1 ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt tctaaaagtt
       61 cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt ttgtgagtat
      121 ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa gaacgcatag
      181 gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat taacagagta
      241 acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga aattgcacag
      301 cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga
      361 ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttccg
      421 ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat
      481 tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct
      541 attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac
      601 agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca
      661 atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa
      721 agcagaatga tagag
//
LOCUS       DAFBQW010000200          731 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFBQW010000200 DAFBQW010000000
VERSION     DAFBQW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.208"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            23..>731
                     /locus_tag="LK889_003119"
     CDS             23..>731
                     /locus_tag="LK889_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5097259.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          257 a          126 c          139 g          209 t
ORIGIN      
        1 gaattataag gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat
       61 aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg
      121 ttcaagacaa acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga
      181 atattatcaa cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc
      241 cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat
      301 tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa
      361 aaaggggcta tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca
      421 tcaagaaaga cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta
      481 tagccaattc tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca
      541 taaaagtgct gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc
      601 gtgtggtaga caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc
      661 gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca
      721 gatcagtaat g
//
LOCUS       DAFBQW010000201          696 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFBQW010000201 DAFBQW010000000
VERSION     DAFBQW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.209"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
BASE COUNT          247 a          116 c           85 g          248 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatta atggaatggc aacagt
//
LOCUS       DAFBQW010000202          695 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFBQW010000202 DAFBQW010000000
VERSION     DAFBQW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.210"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            131..334
                     /locus_tag="LK889_003120"
     CDS             131..334
                     /locus_tag="LK889_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HBK5097260.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            334..684
                     /locus_tag="LK889_003121"
     CDS             334..684
                     /locus_tag="LK889_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK5097261.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          288 a           72 c          116 g          219 t
ORIGIN      
        1 actctcgtca atcattgtta tataataaaa tgttatataa taaaattact aatgatattt
       61 aatattacaa gcaatcttaa gaaattcgaa aaggaaaaaa tgattatgaa aaagaaagaa
      121 aaagaaagta ttgaaacatt gtgttattct aggaatatta ggaacttgtc tagctggcat
      181 tggtacagga atagacgttg acgcagctac ttactatgga aatggtcttt attgtaacaa
      241 agaaaaatgt tgggtaaatt ggggacaatc atggtcagaa gggttaaaac gttggggaga
      301 taatcttttt ggtagtttta gtggaggacg ataatgactt caataaacat tgatcacaca
      361 aaaaaagtca tacatgaatt atacaatagt ttaatgacac gacctaatca gacgaaagca
      421 ttattagata ttactgatgt tttatttcag gtatataaaa aaattgatac agcaaaatat
      481 cctgaaattc ttgtaaataa attagttaat tatatttaca tcgtgggttt tgataataaa
      541 atacgcttta ttgataatga tgaaaaatta ttaatagaat taggggatat ttctaaaaaa
      601 gctggaatta atagtaaata caaagcaaac tttacagata aatcgcaatt ttatagttat
      661 tcagaaacaa tgcctcaacg ataaaatata gtata
//
LOCUS       DAFBQW010000203          616 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFBQW010000203 DAFBQW010000000
VERSION     DAFBQW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.211"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..539
                     /locus_tag="LK889_003122"
     CDS             <1..539
                     /locus_tag="LK889_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5097262.1"
                     /translation="VGILTIPSISLKLPIYDGTSDKILENGVGITEGTGDITGGNGKN
                     PLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIEEVQKDELQRNFVTY
                     LEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEENKKQKLSWKWLLGST
                     VFLSVMIIGSLFVYKKKK"
BASE COUNT          219 a           98 c          124 g          175 t
ORIGIN      
        1 ctgttggtat tcttacaatt ccaagtattt ctttaaaact accaatttat gacggaactt
       61 ccgacaaaat tttagagaat ggtgtaggga ttacggaagg aactggcgat attacaggag
      121 gaaacggtaa aaatccttta atcgcagggc acagtggact ttataaagat aatcttttcg
      181 atgatttgcc ttcagttaaa aagggagaaa agttttatat caaagttgat ggtgagcagc
      241 acgcttatca aatcgatcgg attgaggaag tgcaaaaaga cgaactacaa agaaactttg
      301 tcacttactt agaaccaaat cccaatgaag atcgagtaac gctaatgaca tgtacgccta
      361 aggggatcaa cacacaccgt ttcctagtgt atgggaagcg agtaaccttt acaaaatcag
      421 aattaaaaga tgaagaaaat aagaaacaaa aattatcttg gaaatggctt ttaggaagta
      481 ctgtttttct ttcagtcatg atcattggtt cgctatttgt ttataaaaag aaaaaataaa
      541 ggagggtaac caacatgaga aatagaaaat ggctatttgt ttgtgcagcc gctctttttt
      601 ctttcactgc ctacac
//
LOCUS       DAFBQW010000204          606 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFBQW010000204 DAFBQW010000000
VERSION     DAFBQW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.212"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            77..>606
                     /locus_tag="LK889_003123"
     CDS             77..>606
                     /locus_tag="LK889_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBK5097263.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSK"
BASE COUNT          228 a          116 c           96 g          166 t
ORIGIN      
        1 aatccataca aaaaaggaag tcccttctgt agaataaagt taacgacaac caattcacag
       61 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
      121 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      181 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      241 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      301 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      361 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      421 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac aaaccatgtc
      481 caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag ccttaaatga
      541 taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc aaaccgtatc
      601 caaaga
//
LOCUS       DAFBQW010000205          593 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFBQW010000205 DAFBQW010000000
VERSION     DAFBQW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.213"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(318..581)
                     /locus_tag="LK889_003124"
     CDS             complement(318..581)
                     /locus_tag="LK889_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HBK5097264.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DAFBQW010000206          588 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFBQW010000206 DAFBQW010000000
VERSION     DAFBQW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.214"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
BASE COUNT          192 a           83 c           83 g          230 t
ORIGIN      
        1 aaattataga gccatttttt cttgatgtga tatccttgtc ttttttgtaa tgtttttaac
       61 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
      121 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      181 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      241 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      301 ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga gtaattaatt
      361 agatgtttat tttataggac tgatactata gattacaaat agtatcgtat ttcgcaatct
      421 taaaattatc ttcttactgt gaataagata ataaaatttg tatttgtcct ttttaggtaa
      481 cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat agtcataacg
      541 ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatctttt
//
LOCUS       DAFBQW010000207          583 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFBQW010000207 DAFBQW010000000
VERSION     DAFBQW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.215"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..>583
                     /locus_tag="LK889_003125"
     CDS             <1..>583
                     /locus_tag="LK889_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK5097265.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQ
                     RKTVETVFSSLEKLGCQNFNSRSVKGLESRFES"
BASE COUNT          208 a          108 c           98 g          169 t
ORIGIN      
        1 actttcctag taggagtcga ttcactcgct taagttcaaa cttagcttac actatcaaga
       61 ttattcgata ctttttcatc aagaagctca ccaaaggtga actagtcgga attatagata
      121 gttttcctag tccattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa
      181 tagccaaagt tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata
      241 tgatcgttac taagactggc tttccgatta cttactcaat cacgaatcca ggtgtccatg
      301 acgtgaaagt gttagagact ttatcggaag aagcgaatct tcccaatatc ctaggggaca
      361 aaggatatat tagccataaa atccatgaaa agcttgccct taagggcatt actatatccg
      421 ttccgccacg taaaaacatg gataaatcag aaaaactaga ccacagctta cttggaaaac
      481 aaagaaaaac agttgagact gttttttctt ccttagaaaa attaggttgt caaaatttca
      541 actcacgttc tgttaaaggg ctagagagta gatttgaaag cat
//
LOCUS       DAFBQW010000208          578 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFBQW010000208 DAFBQW010000000
VERSION     DAFBQW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.216"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            126..413
                     /locus_tag="LK889_003126"
                     /pseudo
     CDS             126..413
                     /locus_tag="LK889_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            427..>578
                     /locus_tag="LK889_003127"
     CDS             427..>578
                     /locus_tag="LK889_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097266.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWV"
BASE COUNT          200 a           91 c          122 g          165 t
ORIGIN      
        1 cattggtgca gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg
       61 taacaatcaa taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc
      121 tggttgtcac taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt
      181 ctatatattt tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa
      241 ctgttccaga aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc
      301 gttctacttt gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa
      361 gaaattcaag ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga
      421 aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg
      481 aacaatcaag aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg
      541 agaccaagat ttgattttga taaaaaacag tgggttga
//
LOCUS       DAFBQW010000209          546 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFBQW010000209 DAFBQW010000000
VERSION     DAFBQW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.217"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..347
                     /locus_tag="LK889_003128"
     CDS             <1..347
                     /locus_tag="LK889_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBK5097267.1"
                     /translation="TQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVRSSRH
                     WKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLRRTAN
                     DKKTRSIQHGFI"
     gene            334..>546
                     /locus_tag="LK889_003129"
     CDS             334..>546
                     /locus_tag="LK889_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097268.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDS"
BASE COUNT          215 a           71 c          125 g          135 t
ORIGIN      
        1 ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag aaagacaaaa
       61 aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga gcaggagtgc
      121 gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac gaaattcaaa
      181 aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag aaacttcatc
      241 aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat ttgaggagga
      301 cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat cgaatcagga
      361 gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt
      421 attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca
      481 aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag
      541 gacagc
//
LOCUS       DAFBQW010000210          514 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFBQW010000210 DAFBQW010000000
VERSION     DAFBQW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.218"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..132)
                     /locus_tag="LK889_003130"
     CDS             complement(<1..132)
                     /locus_tag="LK889_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5097269.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAFBQW010000211          497 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFBQW010000211 DAFBQW010000000
VERSION     DAFBQW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.219"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            110..334
                     /locus_tag="LK889_003131"
     CDS             110..334
                     /locus_tag="LK889_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBK5097270.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          221 a           62 c           97 g          117 t
ORIGIN      
        1 attttccgtg acggctcttt ttactatatt aaaatcatag aaatgtctaa tcaaaaaaat
       61 ataaaaatta ttttgcgtac atatgattaa agaaaggaga gtgtgacaca tggaaacaga
      121 atggcaaaga cgtgaaagaa aaaaacagaa aaaagaagag atgttacgat tatgtcgatt
      181 attagatgta aaagactggg acaaaaacga gcaaattgca caaaaagtac gatcaatcat
      241 ccataaagag gaaaaagtta aaaataaaga cacgcgtaga actgaaaatg aaaagagaaa
      301 agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa aaagaggctg agacagaagc
      361 gtttaactcc aagaaataag ctggaattca caaaaatttg aaaagcaatt ttcgtgaatt
      421 tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt tatacgtgtt tagagcgtga
      481 gacaaaagtg ttcttta
//
LOCUS       DAFBQW010000212          487 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFBQW010000212 DAFBQW010000000
VERSION     DAFBQW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.220"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..>487)
                     /locus_tag="LK889_003132"
     CDS             complement(<1..>487)
                     /locus_tag="LK889_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBK5097271.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAFBQW010000213          483 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFBQW010000213 DAFBQW010000000
VERSION     DAFBQW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.221"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..300
                     /locus_tag="LK889_003133"
     CDS             <1..300
                     /locus_tag="LK889_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5097272.1"
                     /translation="AAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKV
                     DKDSYKEENRITIKNNQKASVPMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            313..>483
                     /locus_tag="LK889_003134"
     CDS             313..>483
                     /locus_tag="LK889_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5097273.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAE"
BASE COUNT          172 a           92 c           99 g          120 t
ORIGIN      
        1 gcagcaatcc taaaatcaga taaagagggt cattttgaaa tcactggttt aactgaagga
       61 gattactcac tggaagaaac aaaagctcct gaaaactatc aaaaattaac aaaagagatc
      121 tctttcaaag tagacaaaga ttcatacaaa gaagaaaacc gaatcacgat caaaaataat
      181 caaaaagctt ccgtaccaat gactggtagc aacggtttcc aatcttacgt attgatctct
      241 tgcttgttgc ttggggcagg cgcaatttct gcagttgttt actttaagaa gaaagcctag
      301 aaggatcagc taatgattat cagacaccct aagaaaaagc gaatcatggg taaatggatc
      361 attgctttct ggttactttc agctgttggt gtcttacttt tgatgcctgc tgaagcgagc
      421 gtggcgaaat accaacaaaa tcagcagatt gccgcaatcg atcgaacagg aacggcagca
      481 gaa
//
LOCUS       DAFBQW010000214          482 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFBQW010000214 DAFBQW010000000
VERSION     DAFBQW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.222"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..408)
                     /locus_tag="LK889_003135"
     CDS             complement(<1..408)
                     /locus_tag="LK889_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097274.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKP"
BASE COUNT          179 a           78 c           62 g          163 t
ORIGIN      
        1 cggctttcca aatgatgaaa tacctaaatc taacttatta agaaagttga aatttttata
       61 ttcaggaaac catttattaa tttcatgtgc agcacgtaat gcattccctg gagttgtcaa
      121 agagaaaatt aattctgcta agcttattat tgaaaatact gataacttat aattatatcg
      181 tccattgaca atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac
      241 ttgttcttga ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt
      301 tatttcttct cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac
      361 tggccactga tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg
      421 tattaaacag tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac
      481 at
//
LOCUS       DAFBQW010000215          460 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFBQW010000215 DAFBQW010000000
VERSION     DAFBQW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.denovo.223"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(20..91)
                     /locus_tag="LK889_003136"
     tRNA            complement(20..91)
                     /locus_tag="LK889_003136"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="LK889_003137"
     tRNA            complement(113..200)
                     /locus_tag="LK889_003137"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="LK889_003138"
     tRNA            complement(225..298)
                     /locus_tag="LK889_003138"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="LK889_003139"
     tRNA            complement(312..382)
                     /locus_tag="LK889_003139"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="LK889_003140"
     tRNA            complement(384..459)
                     /locus_tag="LK889_003140"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAFBQW010000216        37191 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.guided.020, whole genome shotgun sequence.
ACCESSION   DAFBQW010000216 DAFBQW010000000
VERSION     DAFBQW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.guided.020"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            196..2385
                     /locus_tag="LK889_003141"
     CDS             196..2385
                     /locus_tag="LK889_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5097275.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2382..4175
                     /locus_tag="LK889_003142"
     CDS             2382..4175
                     /locus_tag="LK889_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK5097276.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4375..4689
                     /locus_tag="LK889_003143"
     CDS             4375..4689
                     /locus_tag="LK889_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5097277.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4710..5087
                     /locus_tag="LK889_003144"
     CDS             4710..5087
                     /locus_tag="LK889_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5097278.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            5097..5870
                     /locus_tag="LK889_003145"
     CDS             5097..5870
                     /locus_tag="LK889_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097279.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5892..7295
                     /locus_tag="LK889_003146"
     CDS             5892..7295
                     /locus_tag="LK889_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK5097280.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7477..8661
                     /locus_tag="LK889_003147"
     CDS             7477..8661
                     /locus_tag="LK889_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5097281.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8658..8948
                     /locus_tag="LK889_003148"
     CDS             8658..8948
                     /locus_tag="LK889_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097282.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8945..9166
                     /locus_tag="LK889_003149"
     CDS             8945..9166
                     /locus_tag="LK889_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097283.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9208..9987
                     /locus_tag="LK889_003150"
     CDS             9208..9987
                     /locus_tag="LK889_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBK5097284.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            10054..10548
                     /locus_tag="LK889_003151"
     CDS             10054..10548
                     /locus_tag="LK889_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK5097285.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            10604..11242
                     /locus_tag="LK889_003152"
     CDS             10604..11242
                     /locus_tag="LK889_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097286.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            11314..11709
                     /locus_tag="LK889_003153"
     CDS             11314..11709
                     /locus_tag="LK889_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5097287.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11693..14146
                     /locus_tag="LK889_003154"
     CDS             11693..14146
                     /locus_tag="LK889_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5097288.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            14143..16170
                     /locus_tag="LK889_003155"
     CDS             14143..16170
                     /locus_tag="LK889_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097289.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            16167..17189
                     /locus_tag="LK889_003156"
     CDS             16167..17189
                     /locus_tag="LK889_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK5097290.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            17206..18135
                     /locus_tag="LK889_003157"
     CDS             17206..18135
                     /locus_tag="LK889_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5097291.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            18380..18496
                     /locus_tag="LK889_003158"
     CDS             18380..18496
                     /locus_tag="LK889_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK5097292.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            18512..20431
                     /gene="tet(M)"
                     /locus_tag="LK889_003159"
     CDS             18512..20431
                     /gene="tet(M)"
                     /locus_tag="LK889_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK5097293.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            20529..20717
                     /locus_tag="LK889_003160"
     CDS             20529..20717
                     /locus_tag="LK889_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK5097294.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20775..21128)
                     /locus_tag="LK889_003161"
     CDS             complement(20775..21128)
                     /locus_tag="LK889_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5097295.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21659..22081
                     /locus_tag="LK889_003162"
     CDS             21659..22081
                     /locus_tag="LK889_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5097296.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            22078..22308
                     /locus_tag="LK889_003163"
     CDS             22078..22308
                     /locus_tag="LK889_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5097297.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22806..23006
                     /locus_tag="LK889_003164"
     CDS             22806..23006
                     /locus_tag="LK889_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK5097298.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            23033..24226
                     /locus_tag="LK889_003165"
     CDS             23033..24226
                     /locus_tag="LK889_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5097299.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(24294..25859)
                     /gene="guaA"
                     /locus_tag="LK889_003166"
     CDS             complement(24294..25859)
                     /gene="guaA"
                     /locus_tag="LK889_003166"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK5097300.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25918..26841)
                     /locus_tag="LK889_003167"
     CDS             complement(25918..26841)
                     /locus_tag="LK889_003167"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK5097301.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            27037..27642
                     /locus_tag="LK889_003168"
     CDS             27037..27642
                     /locus_tag="LK889_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5097302.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27950..28966
                     /locus_tag="LK889_003169"
     CDS             27950..28966
                     /locus_tag="LK889_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK5097303.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            29107..30408
                     /locus_tag="LK889_003170"
     CDS             29107..30408
                     /locus_tag="LK889_003170"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK5097304.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30454..31116
                     /gene="deoC"
                     /locus_tag="LK889_003171"
     CDS             30454..31116
                     /gene="deoC"
                     /locus_tag="LK889_003171"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK5097305.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            31137..31535
                     /locus_tag="LK889_003172"
     CDS             31137..31535
                     /locus_tag="LK889_003172"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK5097306.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31601..32683
                     /locus_tag="LK889_003173"
     CDS             31601..32683
                     /locus_tag="LK889_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5097307.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32895..34457
                     /locus_tag="LK889_003174"
     CDS             32895..34457
                     /locus_tag="LK889_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5097308.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34450..35583
                     /locus_tag="LK889_003175"
     CDS             34450..35583
                     /locus_tag="LK889_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5097309.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35583..36539
                     /locus_tag="LK889_003176"
     CDS             35583..36539
                     /locus_tag="LK889_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5097310.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36638..>37191
                     /locus_tag="LK889_003177"
     CDS             36638..>37191
                     /locus_tag="LK889_003177"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK5097311.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDP"
BASE COUNT        12446 a         6309 c         7506 g        10930 t
ORIGIN      
        1 aaggtttcca acaggccgaa ggcgagctcg aacaaatgct aagccaactg attgagaatt
       61 agaaacaatc tgcagtaggg aagaatcatt tacacaaaat tattgacgct cccttataat
      121 gagaactttt actaacataa tttatgataa acttactata taatcatatt aattaaatag
      181 ttaggagatc tgcttatgtt tatatctact cttcaaattc gaaattttag aaattatgaa
      241 tccgaaatct ttcgattcga taatgagaca aatactatta taggtgagaa tgattcaggg
      301 aaaaccaatg ctcttactgc attgagaatc ttattagatg atagttatta ctatagtagt
      361 aaaacactaa aagaatcaga tttttttcat ggaattcaaa atgggtggca agggcattgg
      421 attattatct ctgcgacatt tgaaggaatc agcgaagaag aatttgacaa tgagatttgt
      481 gcttccattt ctttaaattc tgaaagtcaa acaatcttag aagaattgat ttcaaatgca
      541 gataaaggtg taggtagtat ttctctattt attagaccta acaaagctat tagaaagcaa
      601 ttatttgata tttccactga attagactct catcaattta acgaatttag aaattctatt
      661 cgtctatccg actatgaatt ttactacaca tctaaatcaa atctaaattt ctgtatcaat
      721 gaaaattatg ataaattggt aggaagactc aatgacaata tagctagtaa tcctgaagaa
      781 gacgatgaag ctctgttggg ctcaagaatt gacatgtcag atattttcaa gcatatttcg
      841 gttgtttatg ttgacgctct tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca
      901 gtcaaaagaa tcatggaaac aattgaatca aaaatatcat cagataatgt tgagtcatta
      961 aaaacaatta tacagcaatt aaatgagact attaccagtg ttcctgaaat taggaaaatt
     1021 ggcgaaaata ttaatcggca actaaatagc ataattggtt cagtgtattc tcctaatctt
     1081 cttctttcct caactatgtc agatgatatg ggttctttag caaaatttct ttcaatgaag
     1141 ccagaacaaa atatcgactt agacttacta gggttaggac atctaaatat gatttattta
     1201 gctttgaaaa tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa tattatgttg
     1261 attgaagaac cagaagctca tattcatcat catattcaaa aaactctgtt cgaaggatta
     1321 aatcttcaaa aaaactatac tcagattctc atgaccacac attctgtaca tttagctgaa
     1381 gtgtctgaaa tatctcggat gaatgtatta aaatcgttta atggcaaatc tatcgttatg
     1441 aatccagcaa ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa caatctaaat
     1501 ctaataaacg cagtagagag atatttagat gttaaaagaa atggattgct tttttctaaa
     1561 ggtgttatct tagttgaagg agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa
     1621 ttatttggta taaacttaga cgaacttgga ataggtttga ttaatgttgg tagtacttcg
     1681 tttgaatata tcgctagctt gtttgacgat gatagaatac agagaaagtg tgctattgta
     1741 actgatttag atgaacaggc aatcccatca gaacatcgat tgtataaaag cactgcacaa
     1801 gagaaaggtc aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa caattgggtt
     1861 aattgttttt atgctaatac aacatttgaa attgagtttt tagatgtgaa taaagaaaat
     1921 attctaactt atgtcagtcc aattatagat gacgcattca aagataaaaa aacaataact
     1981 gcttataaag aagaaataca aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa
     2041 ctagccaatc atgttggtaa ggggtggttt gcaattgagt taggtgaaaa aattgacttt
     2101 ttaatcaaaa ttcctaagta tattcttaat gctattgctt ttgcatgtca ggaaatagta
     2161 gatctctcaa tttataaaaa aattctactc catacaatcg acttgtacga ggaaactaca
     2221 gaattgtcag ctttatcagc tagtttaaaa acaagtgata cagctgattt gaatatagca
     2281 ataatttctt tattagagta ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag
     2341 atagaaaatt acacaaaaat actttatgct gaggtttcaa aatgacaaat atcttcgaaa
     2401 aaatcgtaaa tgatttaaat ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt
     2461 atgtaaatgc atgtcctggg agtgggaaaa caagggtact tacaaggaaa attgcttacc
     2521 aagcaattaa acacgatgga tcactaaaaa gaataattgc tatcacatac actaatagag
     2581 ctgctgaaga aattaaggaa cgcctaagtt tacttagtat agatgatgat attaatattt
     2641 gggtggggac aatacatcag ttttgtcttg aatttattat ttatcctttt aaaatgaatc
     2701 tctcaagagt atcaaaagga tttacaatta ttgatgatta tactcaaaga gcttacgtca
     2761 gccaaattaa taatatgctg aatttaaatc tcaaaacgta tgaactgaac aaaattgatt
     2821 tgtcactttc tcatgatttt aaaattaatg aagttcagta tactcaagtt tctgaaatgt
     2881 atcacactat gttggaaaaa aataaagaaa ttgattttga tatgatttta ctagtagcgt
     2941 ataaaatttt aactgtaaac agtaacgttg cacgtaatat tgctagtaat ataagggcga
     3001 tttatgtgga tgaatttcaa gatactagag aattacaata caatgtaata gctaaattac
     3061 ttcaaaacaa ccctcaaatt cagtcaatgt ttgttggaga tattgaccaa gcaatttatg
     3121 gatcattaga tggtatagct aaatcagttg aagaactaga aatgctaact agtcacactt
     3181 ttcaatcaaa gacgctacat ggctgttaca ggtctaatca gagattagta gacttctact
     3241 ctaattttca gagttgtcct tacgagattg aaagcagagg gaataataaa aacgaccgtg
     3301 gttttataag ttatgattgc aaagttacta aggaggattt acccaacata ataaaaaata
     3361 taataaagga aaagattgaa agcggcattc atgaaaaaga tatttgtgta cttgctcctc
     3421 aacactatcc tctgttttca ctgggtgagt atttaaagaa agaattaccg tattgcaatt
     3481 ttgatgctat aaatatatca ccaattaagg taaataatca tagtttattt ttcaagttag
     3541 caattttgta ctttactgaa tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg
     3601 atattttaat tatattaaaa aatgaatttg gaatagaagt taaagattat tttattgact
     3661 tagatttatt acagctaata aacagtgtga aaagaatgta tagtggagac gacaacggtg
     3721 ttgatttata caaatttgtt accaaacata tatttgttaa tcttaatatt gacgagaaaa
     3781 attacccgtc tatttttaag cactatctat tttttattaa cgaagtagaa gatcggataa
     3841 aaaataattc attatctagt agtgtatcta gttttaagaa gatgtttaaa tctcgagatg
     3901 gtatcacact tactacagca cataaagtta aaggaaatga gtatgatact gttatcgcta
     3961 ttaatcttct aaacgggaaa attcctcatt ggaatgaaat ttttaatgtt tctgataaag
     4021 gaattagttc agcgagaaaa ctactatacg taatttgttc aagagcaaaa aataatttat
     4081 ttcttttctc agaggtgggt tattttacga gtaaaggaga cttgctttat cccactcaat
     4141 tattagataa aatagaattt gattataata tttaaaatac ctaggtagtc gttgttttaa
     4201 atatagtagg aatcggacgc tcagtacgga gcgtctattt ttttacccaa ttttgaaagg
     4261 aagtgaactt atgaaaataa aaaatcaaga atcaaaaggt cgctctcccc cctttaaaac
     4321 catcacacaa ataagattca ttcaaacata aaaacagaaa ggataggtaa aaatatggaa
     4381 cttaaatatg tcattcccaa tatggaaaaa acatttggac aattagaata tgctggtgaa
     4441 gggaacattg aacaacgtcg aataaatggc agaccaacca ttctctctcg tagttacaat
     4501 ttatactcaa cgattcaacg agcagatgat attgttgtca ttcttccctc cgaagctggc
     4561 gagaaacatt ttgaatccga cgaaaaagtc aaactcatca acccacatat taccgcagaa
     4621 ggttataaaa ttggcaatcg aggatttacc aattacatta tgcacgcaga cgatatggtg
     4681 aaagcataag aaaggaaagg tgaaaaaata tgagattagc agaaggtatt gtgattgaaa
     4741 aagaccagac atttggcaca ttgaaatttt ccgccttgcg tcgtgaggtt cgtatccaga
     4801 atgaagatgg tacggtttcc gaagaaatta aagaacgtac ctatgactta aaatcaaaag
     4861 gacaaggacg catgatacaa gtcagtatcc ccgcttctgt tcctttgaaa gaatttgact
     4921 acaacgcaga agtggaatta atcaatcctg tggcagatac cgtagcgacg gctacttttc
     4981 gaggggcaga cgttgattgg tacgtgaaag cagacgatct tgtattaaaa ggacagacaa
     5041 aacctcatca gaaatataaa aaagaagcgt ccgaaagtga aaaataggtg ttgcttatga
     5101 caaacacacc attgattttg aaagaaattc cgaaggatga agccatttct tttattcggc
     5161 aataccatta ttcaaagata ctccctagat tgtgtaaata ctttctaggg attttttcag
     5221 aagaaaaatt attaggcgtt gtggaattag gctggggtac gcaaccccta caaaccatac
     5281 gcaaactctt tcccgacagt tcccttcaaa ccacagatta tttagaaatt ggaaagatgt
     5341 gttttcttcc agaaatgaat caaaccaatt attttggcag tcaagctctt tcagctctaa
     5401 ttaagtggtt gaaagaacat acggactgtc attttttata tacactggca gatggaattg
     5461 agggaaaatg tggctatgtc tatcaagcgt ccaacttctt ttactgcggg tatttcaaga
     5521 caagtgtcta tcgtgataag cagtcatggg aaaagataca ccctcgaagt gctcgccttt
     5581 tattagagga aaatgctcgc tttgaacagg tagagaaaaa gcattggctt tcccaggcat
     5641 tttgcgaata caaagggatt gagaaaatca atggacgcat gtttcgctat ctctatccgt
     5701 tgacaaaaga agcgaaaaaa ctgttaggac acactctcta tcgacgtcat tattatccga
     5761 aagaaaaaaa tttacgcttt gaaaagcgaa tcgcttatca gaaatatgaa gccatttctc
     5821 aaccaacttt tgataaacag gctcgtatct acaatacaca attattttga taggtggaaa
     5881 ggaggatttt tatgccattg cttcatgaca gaggagagcg aatcaaagcc aacgataagc
     5941 acttggtcta tcattttgta ctaatttcat tgctggtagt ttttctagtc gtcctactcc
     6001 cttttcattg gagagcgtta caatcggtga actggcaagc agttcagtta gatacatttt
     6061 tctatcaact tcatacgcca tacttttgga gcagtattct cactgctctt tttgtatgcg
     6121 gacttatcag ctggctttat tatcactatc gtatagattc tataaaacag ttagagcacc
     6181 gtcaaaagtt agctcgtatg attttagaaa atcattggta tgaagccgaa gaaactcaat
     6241 cagagggatt ctttaaagat ttacccagca agaaaaccaa acaacgaatc cgccattttc
     6301 ctaaaatata ttatcgaatg aaagatggac taatccatat tcatgtagaa atcaccttag
     6361 ggaaatatca agatcagctt ttgcacctag aaaacaaact agaaacagga ttgtattgtg
     6421 aattagtgga aaaaacgttg aaagattctt atgtagagta tgtcctactt tatgatacta
     6481 ttggcaatcg aattggcatt gaagacgtgg tagctcaaga tggacgtgtc aaattgatgg
     6541 aatcggtcta ttgggagttc gattctctcc ctcacatgtt aattgctggg ggcactggta
     6601 gtggaaaatc ctattttatt ctcacattga ttgaatctct cctgcataca aatgcaaagc
     6661 tttatattct tgaccctaaa aatgctgatt tggcagactt aggggcggtc atgaataatg
     6721 tctattaccg taaagaagat atgttggcat gtatcaatca attttacgaa gatatgattg
     6781 atcgcagtga aaccatgaaa caacacccta actataaaac aggagaaaat tatgcttatc
     6841 ttggtttgtc tgccaatttt cttatttttg atgaatatgt ggcaatgatg gatatgctag
     6901 gtcgtgaaag taccaccgtt attcataaac tcaaacaaat tgtaatgtta ggacgacaag
     6961 caggcttctt tttaattctt gcctgtcaac gtccagacgc aaaatatttt agtgatggga
     7021 ttcgggacaa ctttaacttc cgtgtggctt tgggtcgtat gtctgaaatg ggctttggta
     7081 tgatgttcgg aagcgataca caaaaacaat ttttcttaaa acccatcaag ggaagaggtt
     7141 acgtggatac aggaaaaagt gtgattagtg agttttatac accccttgta ccaaaaaggt
     7201 acgatttctt aggagaaata ggaaaagtca ttcaaaaaaa gcaatctgag cctgtaccgc
     7261 acgaagtgaa aggtacaggt tcagacttgc tttgattggt gtggcgttag ccacgccaat
     7321 cctttagccc cctctatcta acaggggggc acaaatacaa ctgaaaacag gcaattctca
     7381 tcaaccaatg ttgatggtac aaaggattgc ggaaaatatg aaaggtgtaa actcaagggt
     7441 catagtttac atctttttta gattggaggt ttgcgaatgg aaaacgaaaa aatttggatt
     7501 aaggaattga aagaaaagcg actggcttat ggcgtttctc aaaataaact ggcggttgct
     7561 tctcatatta ccagacctta cctatcggat attgaaacag gcaaggcagt ccctactcaa
     7621 caggtgaaag aagatttact gaacgctttg gaacgcttca atcccgataa cccattggag
     7681 atgttattcg actatgtacg gattcgattc cctaccaatg atgtggctca aattatcggt
     7741 gaggttttac gtttgaatat ggattatatg ctacacgaag actttggcta ttattcttat
     7801 cccgaacact atcgtttcgg tgatattgtt gtcctagtct ctcatgatgt aagtaaaggt
     7861 gttttattag aactgaaagg aaaaggctgt cggcaatttg aaaactttct attggctcaa
     7921 catcggagct ggtatgattt ctttcaagat tgcatggaac ataaaggtat tttcaaacgc
     7981 ttggatttag ctatcaacga taaaacaggg attttgaata ttccagattt ggcaaagaaa
     8041 tgtaaacagg aagaatgcat ttccgtcttt cgaagcttta aaaattatcg cagtggtgaa
     8101 ttggttcacc gtgatgaaaa accatacatg ggaaacacac tttatattgg ctcattaaaa
     8161 agtgaagtct atttctgtat atatgagaaa gattatgagc aatacgtgaa aaatgatgtt
     8221 cccttagaag acgcagaagt aaagaatcgg tttgaaattc ggttaaaaaa tgaccgagcc
     8281 acccaagcca tgaaagattt attggcacat caacaggcgg aaaaaacagc ctttgaaatt
     8341 atcaatcgct atatccgctt tgcagataaa gacgacacta aacgtcggag tgattggaaa
     8401 actaatgaac gttgggaatg gtttatcggt aaaaatcgtg gagcattacg cctaaccaca
     8461 cagccagagc cttattcttt tgaacgaaca ttgaactggt tacatcatca agtcgcccct
     8521 acattaaaaa tcgcaagcat tttagatgtt ctcaacggta caaccattat ctccaccatg
     8581 attcaggaag ccaaactgac agaaaaacat gagaaattaa ttgaacaaca acatctagcc
     8641 atggaggatt tgattacatg agccttttaa caaaagaatt aaagaagtta gggtttcagt
     8701 gtggcattga gtttcaagcg tatatacaaa atacagggaa atatacgtct ctcattattg
     8761 agggaaaacg gcaagctggt gataccatct atacctatga tttttacaag gtgagatttt
     8821 ataataatta taccaatcga gtaaccgtct atggagagca tttaacccct tttcaattat
     8881 taaggcgtgt aaaaagctat atctattatc gagaaaaata tcttaaggaa aggaggacaa
     8941 tcacatgaat tttgggcaga atttgtacaa ctggttcttg gataatgcac agtcattggt
     9001 attgttagcg attgttgtca ttggactgtt tttaggtttt aaacgagaat tttcaaaact
     9061 cattggcttc ttgattattg ctctaatcgc cgtcggactt gtgttcaatg cagctggcgt
     9121 gaaagatgtg ttattaaact tatttaaccg aatcattggt gcttaacaga tatttttcat
     9181 gataaaatag gaagtatata ttttgttatg aaaggattta tgaatatgtt tacaaatgat
     9241 caatttaaat ctcttttaat taaaacatta gaaagaaaga gctcttcaaa ttattatgaa
     9301 ggaggtaacg agattgtttc taaaatgaaa atatcagagg taactttaga tgagactgac
     9361 gattttactt attataaagg atataaaaag ggctggaata cgctttgcac atatcttaaa
     9421 ataagagttc cttttgatga tttagacttt tttgaaagtc ataaagattt gattacccag
     9481 acagcaagct ccatatatga taaacaaggt gataatgtat tagtggatac tattttagta
     9541 ccacttccag agaattacga agtaattaac tttagtcagt tgaaaattag tgatgtggtt
     9601 tcacaagcta ttgaagacgc tgagtctttt atgagtaatg gtgaatatca acgtgccttt
     9661 gacagagttc acactgcttt tcatggatat ttaattgaaa tactaaaaaa atatgaaatt
     9721 acagttccta gagacgaaaa tctatctaaa ttatatagta ggatacaaca attaattgaa
     9781 aaagaaattc aacctactga acttgctgat atagtgaaga caacgataag gtcatcaaat
     9841 ggaatgatta gctctcttaa tgaagcaaga aataggcatt cacttgcaca ccctaataca
     9901 aatattattg gaaagagaga agcaaaacta ataataggta tttcaagtac agttacagat
     9961 tatattagcg gataccttga taagtagcgt cattaactga ttgctacttt ttttatgact
    10021 atttttataa aaaggatgtg ataagaattg gaaatgaaag tctatgtggc aaatttagga
    10081 cggtataatg aaggtgaatt agtcggagca tggtttactc caccaattga tgaggaagaa
    10141 atggcagaaa gaatcggttt aaatgaggac tacgaagaat atgcaataca tgattttgag
    10201 cttccttttg atgtggacga atacacgccg attagtgaaa tcaaccgtct atgtgaagcc
    10261 attcaagaaa tcgaaggtac acctatttat aatgaactca aagaaattca aggcatgtgg
    10321 tttagtagct tggaagaatt actggaaaac aaagaggata ttcattgtta tagtgattgt
    10381 gattcgatgg aagatgtcgc ccgttattac gtggaagaaa caggacagct tggtgaagtc
    10441 ccttccaatt tacaaaatta tattgactac caatctttgg gacgtgatat ggaaatagag
    10501 ggtaattacc tagttacctc tcatggggta tttgaatatt gtcagtaagt ttaacaatcc
    10561 gtgttataat taaattaata aatagcacgg agaggtataa attatggtaa atgtatttga
    10621 aaatcttaaa ctattaaaac tagacatgaa agataaaggc tgggtgatag attcctttta
    10681 ctttcgttac aaacagcaaa attatattgt acttgttaag ctatttgaaa aagaggaaaa
    10741 agttccagaa tatgctctgt taaaactaga atttttaaag gaaaacgatt tttcagatat
    10801 gctagctgtc tatgctaatt cggtaaaatt atatacagac acgaaaacaa taagagaata
    10861 ttttggtatc gaatacagta gtaacttggg agatgtttta tttcaattta gccaaactct
    10921 tgctcgattt attccgacag aagttagtga gaagaaaaat gaagaccaaa aagaagccat
    10981 gtgtttctca ttaagtcaaa gtgattcaga agatcccaga aaaaaatatt gtttttcagt
    11041 tagaagaaat cctttaaagg gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa
    11101 taaaacgaga ttgtatagac ccagcttata tgaacgatta gggtcagata ccaatttaag
    11161 tttcagatat tcaatgaatc cagatgatga agaaactgat gaaggtatta ttgcaaaatg
    11221 gacaaaaaat aaaattgaat agttaaagca ttgaaagagc aatcacctat tttgattgtt
    11281 ctttttctat ttttacggaa aggacgtgaa gacatgaaaa aaataagaag ttacaccagt
    11341 atttggtcag tagaaaaagt gatttatgcg attaatgatt tacaactccc tttcccaatt
    11401 acctttacgc aaatgacatg gtttatcgtt tctttgtttg tcgtcatact ctttggcagt
    11461 ttaccaccac tttctctgat tgacggagca ctactcaaat acttagggat tcccattgtt
    11521 ctcactgggt tcatgtgcca aaagaccttt gacggaaaga aaccctatgg atttctaaag
    11581 tctatcatta cctatgctct acgccctaaa gtaacctacg ctggcaaaca aatacgatta
    11641 aaaaaagaaa caatgaatga atcgattacg attgtaagga gtgaacaact agatggcata
    11701 tccgattaaa tatatggaag ataatctcgt atttaatcat gatggcgaag tattcgctta
    11761 ttatgaatta attccctata attacagttt tttaagttcc gatgaaaaaa ttcaagttca
    11821 tgataatttc tgtcaattga ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact
    11881 agcaaccgaa tcaagtattc gagccacaca ggaacaatcg aaaaaagaaa tcactggacg
    11941 attaaaagag atagcttacg aaaaaataga tgaacagacc gaagcgttaa tttctatgat
    12001 tggtgacaat caagtagact atcgcttttt tattggtttt aaactctacc tcaatgaaca
    12061 ggaagtatcc gtaaaaaatg ttgggaaaga aattcaaaaa gcatttgcgg aattctttca
    12121 tggtgtgaat cacaaattaa tgggcgattt tgtttctatg cctatggcgg aaatcgaacg
    12181 cttttctaag atggaacagt tgttagaaag taaattatcc agacgctttc agattcgtaa
    12241 attggagaaa gatgattttg gttatattat tgaacatctt tacgggcaaa caggtgtccc
    12301 ttttgatgac tacgactacc atttaccgaa acgatacgaa gataaaaaag tgttggtaaa
    12361 gaaatacgat attctcaaac ccactcgctg tttaatcgaa gaaaatcaac gctatttacg
    12421 gattgaacag gaagatacca ctacttatgt ggcgtatttt acaattaata gtattgttgg
    12481 tgaattggat ttccctaata gtgaaatttt ttattaccag caacagcagt ttactttccc
    12541 cgttgatacc agtatgaatg ttgaaattgt cgccaataaa aaagccttat ctaccgttcg
    12601 caataaaaag aaagaactga aagatttaga tagccacgca tgggaaagcg acaacgaaac
    12661 cagtactaat gtggtggacg ctttagaatc tgtcgatgaa ttagaaagta atttagacca
    12721 aaccaaagaa tctatgtaca aactaagcta tgtggtacgg gtatcagcaa agaatttgga
    12781 cgagctgaaa caacgttgtg atgaagtaaa agatttttat gatgatctgt ctatcaaatt
    12841 agtacgacct tttggcgata tgctaggctt gcataatgaa tttattccct caagcaagcg
    12901 atatatcaat gactatattc aatatgtcac gtcggatttt ttggctggtc ttggtttcgg
    12961 tgctacacaa atgattggtg aaaattctgg tatttacgtc ggttacaatc tcgatacagg
    13021 cagaaatatt tatctccaac ctagccttgc aagtcaagga attaaaggct ctgtaacaaa
    13081 tgcattagcg tccgctttta ttggttcatt aggtggcgga aaatcattct ctaacaatat
    13141 gctggtctat tattcagtac tctttggcgg acaggcaacc attgttgacc caaaagctga
    13201 acgtggtgaa tggaaagaaa ccttacctga tattgcccat gaaatcaata ttgttaatct
    13261 cacgagtgag aacgacaata aagggttact tgacccttat gtgattttaa aactcccgaa
    13321 agattcagaa agccttgcca ttgatattct cacgttctta acaggtattt ccagccgaga
    13381 tagcgagaag tttcctgtcc tgcgtaaagc tattcggagt gtaactcaaa gcaaacaacg
    13441 agggttgtta ttggtgattg atgaattgcg aaaagaagat acgcccatca gcaatagtat
    13501 tgctgaccat atcgaaagct ttgtcgatta tgattttgcc catctgttat tcagtgatgg
    13561 cagtgtcacc caatccatta gcttggaaaa acaactcaat attattcaag tggcagattt
    13621 ggtcttaccc gattccaata gctcttttga agaatacact accatggaat tattaagtgt
    13681 ggcaatgctg attgtcatta gtacctttgc tttagacttt atccactctg accgttccat
    13741 ttttaaaatt gttgaccttg atgaggcttg gagtttttta caagtggcac aaggaaaagc
    13801 tctatctatg cgattggttc gagcaggacg tgccatgaat gcaggggtct atttcgttac
    13861 tcaaaacgcg gacgatttac tagatgaaaa gatgaagaat aatattggct tgaaatttgc
    13921 ctttcgttcc actgacctag tggaaatcaa aaagacctta gaattttttg gtgtggacaa
    13981 ggaagatgaa aacaatcaga aacgcttacg tgaattagaa aatggacaat gtttagtttc
    14041 agacatctat ggtcgtgtag gggttatgca gtttcacccg atttttgaag agttgcttca
    14101 tgcctttgat accagaccgc ctgtgcgaaa tgaggtggaa gaatgacaca aaaacaaaag
    14161 aaatttcttc actatagttg gataacgctc ctttgtgtgg gcgttatttt acttttatta
    14221 ggcacgctgg gcaatgcagt acaagcgaca ggcttagttg atgaaaccat tgatacaagc
    14281 aatgaatact ccaagtatgg tttgaatcaa tatcagcttg attattatgt ggacaatagc
    14341 tggggctggc ttccatggaa ttggagtgat ggcataggac aatcggtgat gtatggattg
    14401 tatgccatta ctaattttat ttggacaatt tctctttatc tatcgaacgc cacaggttat
    14461 ttggtgaaag aagcctatgc cttagatttc attagtcaaa catcccaagc aattggcgaa
    14521 aacatgcaga cgattgcagg cattacaccg aatggctttt ccagttcagg gttttatgtc
    14581 ggtttcctcc ttctttttat tctcattgtt ggaatctatg ttgcctatac aggattggtt
    14641 aagcgagaaa cgacgaaagt gatacgagct gttttaaact ttgtcgtggt gtttattcta
    14701 tcggcttcct ttattgcgta tgcacccgat tatattgcga aaatcaatga tttttccaaa
    14761 gatgtcagta ccgccagtct gaatattggg acaaaaattg ttcttcctaa cacggatagt
    14821 caagggaaag acagtgtgga catgatacgt gacagcttat tctctgttca agtaaagcaa
    14881 ccatggctat tgcttcaata tggctccact gatgtagaag cgttagggga agaacgtgtg
    14941 gaaagtttat tgtctaccag tccagataca aacaatggtg aagatagaga aaatgtagtg
    15001 attgaagaaa ttgaagatca tgacaatacc tatttaaccc taccgaagac gatttccaga
    15061 ttaggaacag tcttcttttt gtttgtattc aatattggta tctcgatttt tgttttcctt
    15121 ctaacaggaa tcatgatttt ttcgcaagtg ctatttatta ttttcgctat gtttttgcca
    15181 atcagtttcc tattaagtat gttgccgagt tttgatggca tgggcaggca tgccattacc
    15241 aagttattta atgtgattat gacaagggct ggtattacct tgattattac cgttgccttt
    15301 agtatttcca ctatgcttta ttcgctgtca gcaagttctc cctttttcat gattatgttt
    15361 ttacaaattg tgacctttgc agggatttat tttaaactgg gcgatttaat gagcctattc
    15421 actttacaaa gcaacgactc tcaaaacatg ggcagacaag tgtttcgcag acctcgtcaa
    15481 atgatgaatc gacaaaccag aagatttcaa cgcagtatga gtaagatttt tcgtggaaaa
    15541 gcagggtcaa cgaagcaaaa gacaatgaca acaaagcaga atcaaaccaa taaagctaac
    15601 catactcgtc aaaatgaacg gactacacca aagaaagaaa cagcactttc caatgtgaaa
    15661 aagcaacaag gcggtatcgg gcaacgatta ggtgaaaaga cagcccatgt attggacacg
    15721 aaaaatcgtc tagtcaataa aagaaagcag gcaaaacaac agatacaggc gacaccaacc
    15781 catgtcaaat atgcccttca tcaaggaaaa gagaatgtca ctcataatgt aacagacttt
    15841 aaacaatcta tggcagatac caaagctcaa cgcaaacaac agcgtatgaa ccagcaggag
    15901 caaaaacgaa agaacattgc tcaaaagcgt ttggagctgg ataaagcaaa agaaaaaaga
    15961 cgaaaagaaa acccatcatc tcctgttcag aataaccagc agcatgttcg caagcgtcag
    16021 caaaccgtca cgcctgtttt gtcacaaaac actaaacgtg aaaatcatca gcgaccattg
    16081 aaaaaagagc aaaaacaaaa gacaccaccg aagcaattca atcagaggaa gctaaataaa
    16141 agaacggata agcgaggaca acaaacatga agctaaaaat aacagtgatt tgtagtgtgg
    16201 tcttctctct cttctttttt ctcttattat tcctagtcat tttctttgca gatgatacgg
    16261 acagtggcga aaacaacaag gattcttcta ttccacaagg cggtgtgacc gtttcaccag
    16321 aagtgttggc acaccgtccg ttgattgaaa aatatggtaa ggaatatggg attgaagact
    16381 atgtttctta tatcttagcc attatgcaag tggaatcggg cggtacagca gaagatgtta
    16441 tgcaaagttc cgaatctttg ggtttaccgc ccaacagttt gagtaccgaa gagtccatca
    16501 aacaaggggt taaatatttc agtgaattat taaccagtgc agaacaacaa ggagtggata
    16561 tagattctgt tatccaatct tataattatg gcggtggctt tttaaattat gtgaggagtc
    16621 atgggaaaaa atatacctat gaattagccg aacagttttc taaagagaag tcgggtggtc
    16681 aaaaagcaga ctatcccaat cctatagcca tacctgtgaa tggtggttgg cgatataact
    16741 atggaaatca gttttatgtt cagttggttt cacaatacct aaccgacact tccccaacag
    16801 aatttgatgg tgaaaccgtg caagtcatta tggacgaagc cttgaaatac gaagggtttc
    16861 cctatgtctt tggaggggcg tcgcctacca cctcttttga ttgtagtggc ttaatacaat
    16921 gggtgtatga caaagctggc atttctctcc ctcgtgtcgc ccaagatcag tatgacgcta
    16981 cgcaagaaat ctctatggaa gaagcccaag caggcgacct tatcttcttt cattcaacct
    17041 ataatgcagg cacatatgtg actcatgtgg cgatatattt agagggaaat cgcttttatc
    17101 atgcaggcga cccaatcggt tatggcgact taagcagtcg ttattggcaa gaccatctga
    17161 ttggggctag acgtgttatt cataactaag aaaggaatga atcaaatgat tataaaaatc
    17221 gagcgaaaag aaaagaagga gaaaccaaag aaatctaaaa aaccgagcaa gcaaagaaaa
    17281 gttccgatga ttaaagttgg cacgcataag aaattaacct ttgttttatg ggttttattg
    17341 attggtagtg tcggatttgg aatctataaa aattttactg ccattgacac tcatactgtc
    17401 cgtgaaacag aaatcattaa acagcagatt gtcgatacca accaagtaga aagttttgtg
    17461 aaatcctttg cgaaagacta cttttcgtgg caacaatccc aagaagccat tgataaacga
    17521 aatgaaaaat tgacccatta tttgacggaa gaattgcagg tattaaatga agaaatgatt
    17581 cgtaaggata ttcctactag ttctagtgtg aacgatatac aggtgtggca ggtttctcaa
    17641 gtgaatgaga acacctttga ggtactgttt tctgttgaac aagttattac cgaagacaaa
    17701 gacaaggaaa ctatatcgtc tagctttcat gtggttgtcc atatagatga atcagataat
    17761 atggttatta tcaaaaatcc gacaatgagt aagaaacctc aaaaatcgga ctaccaaccc
    17821 aaacagctag aaagtgacca tacagtagat acagaaacga tggacgaaat cactagcttt
    17881 ttagaaacgt tcttccagct ctatcctacc gccacagaaa aagaattgac ttactatgta
    17941 agtaatcatg tgttacctat gattaacaag gaatatgttt tcgaggaatt ggtgaatcct
    18001 atctttacca gaaaagataa ccaagtaatc gtgaatgtcg ctgtgaaata tttagatcaa
    18061 gaaacaaagg caacccaaat ctcgcaattt gagttgatat tagaaaaaca agataattgg
    18121 aagattgtaa aataacaaat attggtacat gattacagat actttgtaat catgtactct
    18181 ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagtg
    18241 actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
    18301 atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt tgtacccagt
    18361 ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta tgctttgtat
    18421 acctatggtt atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc
    18481 ccttttgggt ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
    18541 tcatgttgat gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
    18601 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
    18661 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
    18721 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
    18781 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
    18841 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
    18901 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
    18961 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
    19021 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
    19081 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
    19141 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
    19201 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
    19261 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct
    19321 ttatagtgga gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa
    19381 agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc
    19441 tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac
    19501 aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt
    19561 tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga
    19621 tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt
    19681 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
    19741 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
    19801 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
    19861 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
    19921 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
    19981 tggttggaat gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt
    20041 tagtactcca gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa
    20101 agctggaaca gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata
    20161 tctttcacgg gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact
    20221 gaaaaataat gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg
    20281 caatgattta acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata
    20341 tcaggttacc actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa
    20401 agtaagatat atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt
    20461 ttcttattaa ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg
    20521 agattgttat tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac
    20581 tacgaatacg agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga
    20641 acgaaacttt aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca
    20701 agacgcagag ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat
    20761 ctgaatttta ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct
    20821 tccattaagg atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt
    20881 cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt
    20941 aatgatacaa gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt
    21001 gttaaataac gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt
    21061 ttcattcgag cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt
    21121 ttacgcatac taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat
    21181 agaaaaacat gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg
    21241 atgaatccaa tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa
    21301 tttagtcata ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca
    21361 tgagaaagat aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc
    21421 acctgtactg gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat
    21481 caaagcatat gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata
    21541 tcttttagga tttctcttca tttatgcaac aaatccccct ttcgtgtcgg ggtttagtat
    21601 gaagggagat aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat
    21661 gaaaccatct tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt
    21721 tattgaagat gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt
    21781 ctcgttttct gatatagata actaccttgt caactctttt tctactgttg atacgtacgt
    21841 cactgatttc caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt
    21901 aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt
    21961 tatggatatg agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta
    22021 tcgtcatcga ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg
    22081 aaaacagctt atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg
    22141 gaagcaatta atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt
    22201 cgcatgacag acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga
    22261 atggaaacga aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc
    22321 gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa
    22381 agcatattgt gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc
    22441 aaataaaggc aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga
    22501 cctacaaaat tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc
    22561 tgccatgact ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga
    22621 agcgtcacag gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag
    22681 ctttgctaga gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa
    22741 ctatttgata taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag
    22801 taataatgat aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt
    22861 cagcagacat cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc
    22921 aatctcgaaa gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt
    22981 tttcaaatga aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa
    23041 agacccaata aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga
    23101 ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag
    23161 agaagaatat tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa
    23221 aatgacgttt aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa
    23281 gctcagaaca tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa
    23341 aatggctgtt tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa
    23401 gaagtgtaag tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg
    23461 agcaattgtc cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa
    23521 gcaaaagtct aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat
    23581 ttacaaagaa gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac
    23641 tggaatgcga attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg
    23701 agtactaacc atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga
    23761 accaaaaaca aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt
    23821 atccgaatgg aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa
    23881 cggtattcct acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc
    23941 tggtatccat cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag
    24001 tatgggagaa aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac
    24061 tttaggaacg tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa
    24121 tggagccatt tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt
    24181 tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa
    24241 gataagaaga gccagaaacc cttggtataa gaggatttct agctctccta caactattcc
    24301 cactccactg ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct
    24361 acttcgttta cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag
    24421 tcagcggtca ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta
    24481 cgtccgtcac ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag
    24541 atatcacggt caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt
    24601 acgatttcta gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca
    24661 gggaatggct gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg
    24721 acttcgtctt tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc
    24781 agtccaccta cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata
    24841 acgtcagtat acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt
    24901 gttgcttcgt catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct
    24961 gggtcagata cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg
    25021 ttcaagccga attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc
    25081 agaccgtggt caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc
    25141 ccaacgacac tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact
    25201 tgttcacgga ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca
    25261 cagtgacaaa catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt
    25321 acttctgcat ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc
    25381 ggacaatctt tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca
    25441 ccatggctca tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg
    25501 cttgttactt ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg
    25561 ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa
    25621 ttgaagatct caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca
    25681 gaaagaatga ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc
    25741 aataattcgg aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga
    25801 ctaccgtagt ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa
    25861 tttattaccc ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta
    25921 atattttcgc atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc
    25981 ggctcttcct cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca
    26041 tacatctttt gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata
    26101 attctctgga ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt
    26161 ttcgatcagc acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta
    26221 gatttgctga ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg
    26281 ctgctggatc aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg
    26341 tacttttatc tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat
    26401 atcataactt tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt
    26461 tggataaaga aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga
    26521 caaaatcgtc tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat
    26581 cccgataata aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa
    26641 caaagattca aattctttca tataaagata aatcagatga gcaagaggta catagatatc
    26701 tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg
    26761 tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta
    26821 gtagttcatt ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta
    26881 acacaaaata aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct
    26941 ttataataga aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag
    27001 actgttgtca tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa
    27061 cgcccagata cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt
    27121 caatttgtaa cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc
    27181 ctgattgatg ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga
    27241 tgcgggtatg gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg
    27301 gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc
    27361 gaacatgttg atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca
    27421 gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca
    27481 ggcgcatatc ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa
    27541 ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca
    27601 aaagataaag gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg
    27661 agcgacgaaa gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt
    27721 gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt
    27781 cgttgttttt tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg
    27841 gtagataaag gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg
    27901 ggggttttgg tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac
    27961 catcagagat atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa
    28021 taacaaagga agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga
    28081 acatcattat aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat
    28141 cggaatgatc gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga
    28201 gagctatttg aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga
    28261 aaatgagatc aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc
    28321 cacaccaaat attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc
    28381 aatcatttta atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga
    28441 atatgagggc gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg
    28501 catgctgaag gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca
    28561 tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac
    28621 tgtccaaggt ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat
    28681 tttttgcggg aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa
    28741 aaaaatccca gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat
    28801 gattccgcaa ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg
    28861 tttcctgatc gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac
    28921 aaaattaatt attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt
    28981 gcttatgtaa gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt
    29041 actaaagtgg tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat
    29101 caggcaatgc gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa
    29161 gaggaaatcg agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc
    29221 agtgcattat tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta
    29281 acattggcga ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag
    29341 gtagataagc attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta
    29401 gtagctagtg tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt
    29461 ggtacactag acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa
    29521 tttgtacgca ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca
    29581 ccggcggaca aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg
    29641 ctgattgcca gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg
    29701 gatgtgacca ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca
    29761 aagaccatga cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat
    29821 atgtcagaac ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa
    29881 acattacaag gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa
    29941 atggttgttc ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa
    30001 gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga
    30061 gacccagcga tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt
    30121 cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca
    30181 atgatgctag gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg
    30241 aaactgcata aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat
    30301 agtaacgacg aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt
    30361 gaatcggcaa tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg
    30421 gactttaccg ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat
    30481 actattttga aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa
    30541 aaataccatt tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa
    30601 ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca
    30661 cccgagacaa aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat
    30721 atggtcatca atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc
    30781 gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg
    30841 ttattagata gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat
    30901 tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg
    30961 atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa
    31021 gaagaagcaa tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta
    31081 gcaatcgtat ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg
    31141 cagaactcaa acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc
    31201 cttattccca ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag
    31261 gcatcaacat tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt
    31321 tcaaagcagt atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc
    31381 ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg
    31441 atatgccggt tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac
    31501 tgctgcctta tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact
    31561 gttgaaaaat aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt
    31621 tggtttaggt gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg
    31681 ctctactgat tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac
    31741 agacacaggt ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa
    31801 atgggggaaa gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc
    31861 aaatgaatca gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat
    31921 ttttggtatt ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg
    31981 tacgaacttt gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac
    32041 tttcaaagac aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac
    32101 aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg
    32161 attcaaagcc ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa
    32221 tcaatatgct ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta
    32281 tgcacaaaac gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca
    32341 agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg
    32401 cgaccaatca gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac
    32461 atctacactg aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg
    32521 taaattccct ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac
    32581 agaaggacaa ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat
    32641 ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct
    32701 tgttagacac atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc
    32761 aagctgcctt gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa
    32821 agaagaacgc tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag
    32881 gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag
    32941 aatttggaac attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc
    33001 atgcgttgct tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat
    33061 tattagagcc gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc
    33121 cgaccaaagc caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg
    33181 cctttactgt aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg
    33241 accataaaaa agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca
    33301 acccagatgc ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa
    33361 aaacacttta ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc
    33421 ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga
    33481 tcattttgat tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta
    33541 tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag
    33601 ccgatatgat ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc
    33661 aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg
    33721 tgaaaaactt gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg
    33781 gaaacgggca gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac
    33841 atatcaagct aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg
    33901 gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat
    33961 ctgaaaatat cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga
    34021 attattcagt aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa
    34081 cgaatgaact tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg
    34141 ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg
    34201 atgtaggtgc gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag
    34261 cagtgttact gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg
    34321 ttatccatga aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat
    34381 taggtctatt gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg
    34441 ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca
    34501 gttgttttag gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc
    34561 caaggttatt cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg
    34621 ttaaggcaag caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct
    34681 gggtttttca atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg
    34741 gtggcacttg cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta
    34801 ggtgccttag ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga
    34861 acaagtgaag tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt
    34921 gctttacatg aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc
    34981 agtcaaaatg cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta
    35041 aactcaggat tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa
    35101 acgactttag gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca
    35161 ggaatgagta gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg
    35221 cttggcggtg tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg
    35281 ctatctattg gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt
    35341 attcttttat cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca
    35401 ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc
    35461 gggatcaact tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa
    35521 gagatcgttg cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta
    35581 taatgtcgac tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac
    35641 cgctaattct aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag
    35701 ggctagaggg aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga
    35761 gtccatcact tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta
    35821 tcctattttc tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca
    35881 gcgggacagt aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt
    35941 atggtaaagg tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat
    36001 tagcagatat tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt
    36061 gtgctatttt gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc
    36121 gcctccgttc agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg
    36181 ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt
    36241 ttgctcagtc gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct
    36301 ccatggctgc tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt
    36361 tctttggttt tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga
    36421 ttccgccagt attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt
    36481 tcttagggaa agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat
    36541 cttttagcga ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt
    36601 taaaggcaat aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat
    36661 cgtcatggat tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg
    36721 ttctgataca ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca
    36781 gcttggttta ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg
    36841 ctatgcgacg aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga
    36901 aatcatggga ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga
    36961 tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag
    37021 cggaacagct gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt
    37081 atatacttct gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga
    37141 agaattatat cggatttgcc aattcgttcg tgatataaca aaagatgatc c
//
LOCUS       DAFBQW010000217        18331 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.guided.055, whole genome shotgun sequence.
ACCESSION   DAFBQW010000217 DAFBQW010000000
VERSION     DAFBQW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.guided.055"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..646
                     /locus_tag="LK889_003178"
     CDS             <1..646
                     /locus_tag="LK889_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK5097312.1"
                     /translation="EHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSFYSKHMNF
                     SSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGYLTYEEYL
                     KKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPENIFLLRER
                     DLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(796..1737)
                     /locus_tag="LK889_003179"
     CDS             complement(796..1737)
                     /locus_tag="LK889_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK5097313.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1863..2984
                     /locus_tag="LK889_003180"
     CDS             1863..2984
                     /locus_tag="LK889_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK5097314.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3242..4744
                     /gene="eat(A)"
                     /locus_tag="LK889_003181"
     CDS             3242..4744
                     /gene="eat(A)"
                     /locus_tag="LK889_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK5097315.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4772..5338
                     /locus_tag="LK889_003182"
     CDS             4772..5338
                     /locus_tag="LK889_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5097316.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5549..6610
                     /locus_tag="LK889_003183"
     CDS             5549..6610
                     /locus_tag="LK889_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5097317.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6613..7509
                     /gene="murQ"
                     /locus_tag="LK889_003184"
     CDS             6613..7509
                     /gene="murQ"
                     /locus_tag="LK889_003184"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK5097318.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7523..9505
                     /locus_tag="LK889_003185"
     CDS             7523..9505
                     /locus_tag="LK889_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5097319.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9570..10016)
                     /gene="nrdI"
                     /locus_tag="LK889_003186"
     CDS             complement(9570..10016)
                     /gene="nrdI"
                     /locus_tag="LK889_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK5097320.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10227..11651
                     /locus_tag="LK889_003187"
     CDS             10227..11651
                     /locus_tag="LK889_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5097321.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11832..12605)
                     /locus_tag="LK889_003188"
     CDS             complement(11832..12605)
                     /locus_tag="LK889_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5097322.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12602..13507)
                     /locus_tag="LK889_003189"
     CDS             complement(12602..13507)
                     /locus_tag="LK889_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5097323.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13661..13981)
                     /locus_tag="LK889_003190"
     CDS             complement(13661..13981)
                     /locus_tag="LK889_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK5097324.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14455..15141
                     /locus_tag="LK889_003191"
     CDS             14455..15141
                     /locus_tag="LK889_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK5097325.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15143..16087
                     /locus_tag="LK889_003192"
     CDS             15143..16087
                     /locus_tag="LK889_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK5097326.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16286..16930
                     /gene="pcp"
                     /locus_tag="LK889_003193"
     CDS             16286..16930
                     /gene="pcp"
                     /locus_tag="LK889_003193"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK5097327.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17009..18286
                     /locus_tag="LK889_003194"
     CDS             17009..18286
                     /locus_tag="LK889_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5097328.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5873 a         3245 c         3916 g         5297 t
ORIGIN      
        1 agaacacgag gcgatgctga agcaggcaca agatgaaccg gcaatcgatg agatttatca
       61 ttttgatttt ttggaagcaa aagaacttgg gctgaaacat aatagttcat tttattcgaa
      121 acatatgaat ttttcttcta gcgaaccaaa gacggatcta ttttttggtg caacgaataa
      181 tgggagaaaa gaacgagcag aaagctataa gcaggaattt ataaaaagag atgttacgac
      241 caactatttc attcttccac atcgagggaa tgaacaacct ggatatctta cttatgaaga
      301 gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg agggtcagca
      361 aggagtgact ttgcggacat ttgaatccat gtattttcaa aagaaactag tgacggataa
      421 tcaagcgatt gctcattatc atttttatcg tcctgaaaat atttttttgc ttcgagaacg
      481 tgatttagat gaattaccgg aatttctgca tacaccattt aagccaatcc tatcagagct
      541 attggaattt tatgatgcag aaaattgggc aaaacgattt atcactaacg attccactat
      601 ttatgagcaa tacgaatatg acctctcctc tgttgaaaac aaataaaaaa cctacatttt
      661 gaagagatta tgtactgaaa caaacaagca caattcttat ggcatcatga gagttgtgtt
      721 ttctttttga tcttttattg ggcttaaaaa aagagaacca gctgctgatt cagctgattc
      781 tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga ctaattgggt
      841 caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc aaatggcacc
      901 attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt acatagatgg
      961 gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt acacttcttc
     1021 ttgatctagt atgattcctt cttttgctgc gatagaagca aattcagata cgattgttcg
     1081 aaccatgctc tctgccgctt tttgcgcgcc tagttcagca atattacaat ccaaaatcgt
     1141 gcataaacca ttcaatgttc catttacaca agcttttcgc caaatggaat aacgaacgtt
     1201 atggctatag atcggattta aatttgcttc ttgaaagact tctacaactt tttttgtaaa
     1261 tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta acaatgtgac
     1321 ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga tatttttctt
     1381 tggcacatat ttttccagta catcttcatg acctaatcca tttagtaaac acaaaacata
     1441 agtattgtca gtaataatgg attggatcga acaaaacata tcatctaatt gattggcttt
     1501 tgttaatgct acgatcaagt ccacgtgttc gttgctttcg atgatttctt ctggtgaata
     1561 gattggcagt tttgcgacca cttctttacc gttaaagtca gcaatcaacc catttgtacg
     1621 aatggcttca atgtgagcag gccaacgatc gatcaagagg acctcatttc cgctttgatg
     1681 tagcatcaat ccgattcggc tgcccattgc tccggcccca gctattgcga ttttcatgtt
     1741 ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaatgga agaaaatagc
     1801 attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag tgagggatac
     1861 agatggatat tttggcggca attgattcat ttaaaggaag tgcttcttca tttgaactaa
     1921 atcaggcagt tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg
     1981 cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcagggga acatggcgta
     2041 ccattgatgg gatggatctc ttattccgtc agcgaaaaat cagctattta ttgacaactt
     2101 ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc attggggtta gatctacttg
     2161 ataaaccaac ggatcaaact atccgacttg cttccagtta tgccttagga gaacttatta
     2221 aagatgcact gcttcaagaa gtagaacaaa ttattgtcac tttaggagga agcgggtgta
     2281 gcgatggtgg tctaggatta ctccaatcct tgggtgcttc tttagaaggg tacacagaag
     2341 gaaatccttt attatctact aaaaaacttc attttgatcg aagaaacgtt cttgtttcta
     2401 aagtaaaatt gattatcgct gcagatgtta ccaatccata tacaggtcaa caaggagctg
     2461 cttggcaatt tggaagacaa aaaggaggaa tagatacgac gctagctttc ttagataaac
     2521 aagcaggaaa gattgctcag caaataaaag atttctggga ggtggattta gatgatattc
     2581 caggaagtgg agcagcaggc ggtattggtg gagccctttt tttgttggga gcgaagatgg
     2641 tttctggttt cgatttagtc agccaaatca ctggattgga aaaaaagatc gaacaagcag
     2701 atgtcatcat tacaggagaa gggcggatcg atcagcaaac gatccacgga aaagttccat
     2761 acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta
     2821 gagaccaaga gctagaagga ctttctgatt ttctcccaat tgtcttaagt attcatttgg
     2881 gtccaatagg tttagaagaa gcaatggaac acaggcaaac attagaaaaa acaaaaatct
     2941 taggacagac acttagcaga tttttcacca tcctcccgaa gtagttgaca aaatcatttt
     3001 ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca ttgaaaaatt
     3061 aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaagga agcagtgtgc
     3121 agattttcgg gattggcttg gttttttcga tgtatgggtc tttttgtgtg tatccaaccc
     3181 ttgcatcctc tttagccagt tctgaaaata ggactggtct ttttttacga ggtgatggaa
     3241 aatgtctaaa atcgaaataa aaaatctgac attcggctac gacagccaag gcacattatt
     3301 atttgaacaa gcaaatctaa attttgacac acaatggaaa ctaggactta tcggacgaaa
     3361 cggtcgagga aagacaactt tactgaatat tctacaaaac aaactacctt atcaagggca
     3421 agtaatccat cagcaagaat ttgcctattt cccgcaacag acaaaagata aagaacgttt
     3481 aacctattac gtgttaaatg atattacgga ttttgagata tgggaaatcg aaagagagct
     3541 ccaattgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac tatcgggggg
     3601 agagaagaca aaagtcctac tggcactttt atttgtggat gacactcatt tcccgttaat
     3661 cgatgaacca acgaatcatt tggatatctc tggtagaaaa caagtagcgg cttatttgaa
     3721 aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt
     3781 ggaccatgtt ttagcaatcg aaaaaagtca actggaactt tatcaaggga atttctctat
     3841 ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa
     3901 aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg
     3961 agaaggagat aaaacaaaga aacaagtcgg attcatcgat actgaatcta gacgagtgaa
     4021 taaaggagca gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa
     4081 tcggatggag acccagatca gcgagaaaga aaaactatta aaagatatcg aatatatcga
     4141 ttctttgacg atgaatagcc aagcgtctca ccataagcga cttttaagcg tagaagatct
     4201 tcaattaggg tatgaaaatc tgttattcga gccaattcat tttacaatcg agcctcatca
     4261 gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca tccattatcc attatcttct
     4321 gggggcattc aacggcaagg ttataggaga aaaataccag ccaaaacatc tgagcattag
     4381 ttatgcaagc caaaattatg aagacaatcg aggaacgttg gcggaatttg cagagaaaaa
     4441 ccaagtagac taccaagcat ttttgaacaa cctccgaaag cttgggatgg aaagagatgt
     4501 ttttcataac aagatcgagc agatgagtat gggccaacgg aaaaaagtgg aattggctaa
     4561 atctttatca cagccagctg aactatatac atgggatgaa ccattgaatt atttggatgt
     4621 cttcaatcaa gaacaattag aacaactgat cttgaacgtg aaacctgcca tgttactagt
     4681 ggaacatgat caaaccttcc tggataaagt atctactgag attatttctc ttgagagaat
     4741 ctaacccttg taatctcaag aacaacaagg tatggttaag gggaactttg ccaacgaagg
     4801 agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg cacaaggatt
     4861 agtttgcgga cgggcagatg tgccgctgac tgaaaaagga cagatgcagg cacagaaact
     4921 agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt cccctcttca
     4981 acgagcaaga gatacagcac aagcagttgc tgatcgtctc agcttgccgc tgaccgttga
     5041 tgaacgattg gtagaaatgg attttgggga ttatgatgga ctgccaagca aagatgagaa
     5101 ctttcaaaaa gcacgtttag cgttcgctgt acgattccca aatggggagt cagtcttaga
     5161 tgtgtatgca cggatcgttc ctttattgaa agaatgcatc gaagacgaag aaaatgtcta
     5221 tctgttagtt tgccataatg ccttgataag agtgatcaat gcttattttc atccgatgcc
     5281 taatgagggg ttctttacat tcatggtaga taatacagaa ttggtttctt atgagtaatt
     5341 atgtttttgt gttatttaaa taaaatattt caaggcactg cttttgctta gcagtgcctt
     5401 ttttaggtta tgacaattct tttgataata taaactcaac ctttagaaat gaaatttaat
     5461 tattttaata aaagtattga aattttgttt ctttagatat atactctagt catagaaatg
     5521 aagacggata cagaaaaaga gggataatat gtttggcttt tcaatattta tgaacgattc
     5581 tttgtcagaa gagaagaaac agtatatccg ccaaatggca tccaatggct ttgtcggtat
     5641 tttcacttcc atgcatatac cagaagatga tgtttcggca tatcaaaagc gattgatcga
     5701 actaggaagc gaagcgaaaa agaataaact agaattgatg gtcgatattt caggagaggc
     5761 attggaacga gcgggttttt cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac
     5821 aggtttaaga atggattatc atatttccaa tcagcagatt gccgactggt cacatcaatt
     5881 gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat atcgatgaat tgaaagaagc
     5941 agaagctgat ttctcacaga ttgaagcttg gcataattat tatccacgtc ccgaaacagg
     6001 gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt
     6061 ccaaggtttt gtaccaggag atacagagtt gagaggccca ctatatcaag gattgccaac
     6121 tttggaagaa catcgaggga tccatcctct tgcaggcgct ctagatctat tagcaagtaa
     6181 cacggatatt gtttacatcg gagatgcagg tctgtcagaa aacgttcagg agcaatttgc
     6241 ttctttccaa aaagaacaga cagtcttgct ccatacggaa cctgtagatg aagaatttta
     6301 cgaatatata ttagggaagc atacgaatcg gcaagacgat gcaagagatg tcatacgaag
     6361 cgtagatgca cgttttcgag agattccgcc tatcccagca agaaatacag ctacgcgtat
     6421 gaaagggtct attactttag ataacgaaaa gtatttgcgc tatatgggtg agatccaatt
     6481 aaccaaatat gatttgccag cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga
     6541 agagctacca ttgatcaacc aaatcaaagc tggtatgaac tatcaattta taagaaaaga
     6601 aggaagataa aaatgagcaa aatcaattta gagaacctaa caacggaacg cagaaaccaa
     6661 gaaacttttg gattagatga gatgagtatt tctcaagccg ttcaactaat gaacaaagaa
     6721 gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct
     6781 aacacgattg agtcctttaa aaaaggtgga cgtctgattt atatgggagc cggaacaagt
     6841 ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa
     6901 atggtcatag gtctaattgc tggtggagaa gaagcaatga ctgtagctgt cgaaggggcc
     6961 gaagattctt atgagctagg gcaagaagat ctaatcaagc tgaatttgac aaaaaatgat
     7021 atagttattg gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg attagattac
     7081 gcaaaatcga tcggtgctgt cacaggaacg atttcttgca ataaacaagc cgaaatcagt
     7141 aaacacgcgg atttcccaat cgaagtcgat gcaggtccag aatttttgac aggctctact
     7201 cgattgaagt caggaacagc acaaaaactt atcttgaata tgatttctac tatcagtatg
     7261 attggtatag gtaaagtatt caataacttg atggtcgatg tcaaaccaac aaatgaaaaa
     7321 ctaatcgaac gctcaaaacg aatcatcatg caagcgacag aagctgatta cgaaacagct
     7381 gaaaagtact ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat gattttgaca
     7441 aatagtgata aagaggaagc tcaagcaaaa ttagctgaag caaacggatt tgtaaaaggg
     7501 accttatgag ggggaaaaga ccatggcaaa aaacgaactg actttggaag aactggcaaa
     7561 acaaatttat aaaggggcag gcggcatggg caatgtagaa agcattgtcc actgcatgac
     7621 tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat gtctctgaat taaaagcgat
     7681 tccaggagtg cttggtgtag tagaagatga acagcttcaa gtcatcatcg gacctgggaa
     7741 agtgaacaaa gtagcacagc aaatggttga tttggcaggc gtgaaattag gagaaaaact
     7801 ttcttctgta agtacagcta cagcaaattc atcagcatct ggaaaagacc gtgtaaacga
     7861 acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa aaaccatcta aaatcaaatc
     7921 ggtattaaaa gacatttcta atatctttgt gccaatgatt cctgcatttg tcggtactgg
     7981 gatcgtggca gggattgccg cagttttagc aaacttagtc acagccggaa cacttgatgc
     8041 agcaacatgg caacaatata tcgatgtgat gaacatcttg aaaaacggga tgtttagcta
     8101 cctagttatc tatacaggta tcaatgctgc acaagttttt ggtgcaacac caactcttgg
     8161 tggagttatc ggggccgttg tgatgctgac agggatgaat ccggaagcac cactgaaaaa
     8221 cttatttact ggtgaagctc ttgcagctgg acaaggtggg atcctaggtg tcatcttcgc
     8281 tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg atgcggtaga
     8341 tatcatcgtt acaccaactc tttcgttgat tgccatcggt ctgattgaaa ttttcttcat
     8401 catgccttta gcaggtttca tttctgatgg catggtcggt gggatcaact gggtacttgg
     8461 cgttggtgga gcatttgccg gcttcgttct aggcacatta ttcttgccga tggttatgtt
     8521 cggactgcat caaattttga ctccaatcca tgtgcaaatg attgatgaaa ctggtcgtac
     8581 gctattgctt ccaattcttg cgatggctgg tgcaggacaa gtgggtgcag cattggcatt
     8641 atggattcgc tgcaaaaaag ataaagaatt aacagaaatg atcaaaggag ctttaccagt
     8701 cgggatccta ggaatcggcg aaccattgat ttacggagta acattacctc taggacgccc
     8761 atttattact gcttgtatcg gtggaggtat cggcggggca gtgatcggaa tgatcggaaa
     8821 tgtcggggct atcgctattg gaccttcagg agccgctttg atcccattga tctctgacgg
     8881 taaatggtta ggctacatcc ttggattatt ggcagcttat gctggtggct tcgttgctac
     8941 attcttcttc ggtattccta aagaccgttt agaaaaagaa gaattagcga ctgaaacagc
     9001 cacagtaaat gaagtgcctg aaaaagtagt acctcaaact tcagctgaaa gtgtgactat
     9061 cctctcatca gttgctgacg gaacagttgt ttctttagat aaagcaagtg atccagtttt
     9121 tgcacaaaag atgatgggtg aaggctactt tgtagaacca gaaaacggac atatctattc
     9181 tccagttact ggggtagtca gctctgtctt cccaacgaaa cacgctattg ggatcacaac
     9241 gccaaatggt ttagaaatct tgcttcatat gggaatcaac acagttgact taggcggtac
     9301 gccatttgat gtgaaagtaa cagaagggca gcaagtgaca cctgatacgc ttgtagcaga
     9361 agctgactta gaagcgatca aagcagcagg caaagaaaca tccatgatcg tcttggtaac
     9421 caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcaa aagcaaaagc
     9481 acctgtgatg gatgtcaaag cttaatatga tagaaacaat agaaacgaaa gaagaaactg
     9541 aaatctattt ttcagcttct tcttttttat tatttattgt tttctgctaa aatctgataa
     9601 attcgtgtca catcagatgg tgttccccgt aattcataat ctgccaagaa aggaaagcca
     9661 aatgtttgtg catattgttt ggctgttaga caatactgat tattgaagtt tttatttccg
     9721 ctaccgacta caccaagaca aagtttatga ttgtcttcat gttccagata ttcacgcatc
     9781 gtttcggtca agatttctgt atcaccgtta tctaaaccat ttccgccgtc taaataagta
     9841 ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat cactattttc atggatttct
     9901 tttaaagtaa cttcaggatt gacaggatct tctgcatgca ttttttcagc atattctgct
     9961 agatgtttgg caaaggcacg cgtatttcct gaaatcgata tatataaaat gttcatggat
    10021 catcctccaa ttctttcctt ttagtatagc atctttttta agttttttgg tggtgtaatt
    10081 tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa agccggtaga attatatttc
    10141 tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt tgttatactt tttgataaaa
    10201 gcggttttat tttggaggat gcttacatgg atacagagca acagaaaaaa ggaattggtt
    10261 taattccact tgctgcctta gtaattggtt cagcgattgg aggtggcgta tttggtatta
    10321 tgacggacat ctccggatcg gctggcggcc cagctatttt cagttgggta ctcgtaggaa
    10381 cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt ccagatttaa
    10441 aaggcgggat ctttagttac gcagaagaag gttttggccg tttcgctggt tttatcagcg
    10501 gttggggtta ctggctcaca tgctggttag gcaacgtagc ttttgcaaca ttgttgatga
    10561 gcgcattagg atatttcttc ccagtatttg aaggtgggca aaatctggca tcgatcgtga
    10621 tgagtacagt ctttttatgg ggatatgcat ggctagtgaa tcggggagtc gaaaatgcta
    10681 gtttcgtcaa tgctatcgtc acattctgta aattagtgcc attattttta tttattgtag
    10741 tagctatcag cacgttttcg atccaacaat tcattgataa cttttctagc gacattatat
    10801 tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt attgagttgc atcatggtga
    10861 tgctttgggt atttgttggt attgaaggag cctctgtcgt aggttcacga gcaaaaaaac
    10921 aatcagatgt tgggaaagca acgattctag ggatattcgg cttgattatt atttacgtct
    10981 tggtttccat gcttccatat gggacaatga cgatgaatca gctgcaaacg atcaaggcgc
    11041 agcctgctat gggctatgtg tttgaaatga tggttggtaa atggggagca gtcgtgatca
    11101 acggcggttt gatcatttct ttagttggtg tttggctctc atggacgatc ttaccgattg
    11161 aaacaatggg aaacatggct gaagacggac ttttgcctaa agcatgggga aaactcaaca
    11221 aaaatggggc accaacttat gctatcgtat tgacgacgtt atgtacgaat gtctttttac
    11281 tttcattatt attcactgac agtgcttata actttgctta cacacttggt acagcagcta
    11341 ttttcttcac gtggcttttc ttaggattgt accaaatgaa actttcttat cagcggaaag
    11401 aatgggtcca atttttagta gggcttttag caagtgcctt tcaagtatgg gctatgctag
    11461 tcgtagcctt taatgaagta atgcttgttc ttgttctttt cttgccaggc atttattttt
    11521 acctcaaagc acgtaaggag caaaacgagc ctgtacaaca tatccactta gataagattt
    11581 tacttatagg tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc actgggatgc
    11641 ttcacgtata gaagtataaa agaagtataa agagaggttg tgagcctgtc gtttattcaa
    11701 aaacatacta ggaaaggagc tgtgacacaa atgttgtcac agctcctttt tcagaataaa
    11761 cggtgtttaa gagacagcca atcggcaata aggctgaacg tctctttcac agcctctttt
    11821 ttgatgagtc atcatacatt ttttcgctta aatacgagat atcctaatgc taagaacaaa
    11881 acgatgtaag ctaaattacc gaatagcatt tgattagtag aaagatgagt caattgcttc
    11941 atgatctctt cgtttccaat ttggttttgc agattcaaca tactgatcgg attccatttg
    12001 atccactccc atttttggat gagtgcaata aggataccag atatgattga cgaagcaaaa
    12061 taaaagacga ttcctgctga gatggatgcg ccggaactat tgataaagca agcaatcatc
    12121 aacactaaac ttaaaatcag ccataaaccg atataactac ccaaagtata agtgaacaag
    12181 ctttggatca gtgtttgacc agtagaaacc ttctccgtga aggaaatcga agggaaaaga
    12241 atcagtttca tcaacacagt gacaataata gctaaaaggt ataaataaac agaataaata
    12301 actaatgtga tccatttgct tactaatatt tctcctcgag aatactttcg ataaaggaga
    12361 tttttcaatg taccatattg tgcttccatc gaaatgatcg tgcttgcagc tgcaatcatg
    12421 ataaagacaa tccaagatgt ggcactaaaa agctgagcag tcatttcaac tggatcaatg
    12481 atcgtcgtgt attttttggc tagaagtgct gttcctatga gcaaaacaac taatagaaca
    12541 gataaaacag cagttgactt tttcttgatc aatttaaata attcttgttt tacaagcaat
    12601 gtcatttttc ttcttccccc tttactgaat ccagaatttc caatagagaa gattccaaat
    12661 cattcgtcag atgctggacg tctgtcacat ggatattttg tgcagacaat agctggatca
    12721 cggttcctaa taattcttcg ttttctttta aaagcttgat tttatcagga tcttccaaag
    12781 tatatccagc ttctgtcaag atcgttcgag cagctagatc atttgttgta gagataacga
    12841 tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg atctttcctt
    12901 gattgatcag gatcagctca tcgaccattt tttcaagctc gctgagtaag tgactggaga
    12961 tcaagaaaga ggtgccctga gtagccaagt cggtaatgat ttttcgcaca tctcttgttg
    13021 cttgtggatc caaaccgttc atcggctcat ctaaaataac gatagacgga tggttcagta
    13081 aggcaagagc aattcccaat ttttgtttca ttcccaacga gtaggatttc gcttttttgt
    13141 gaatgtactt ttccatcttg agctgattca cgatagtgtc gatagcagat acatcgttgg
    13201 tttcagagaa taacttcaga tgatcatagc ctgttaagaa tggatagata cccgggtatt
    13261 cgatcaatgc tcctacttgt tccaaccctt tatgactaga gggagaaatc tcattttcac
    13321 caatacagat tgttccatct gagtaatgga tcagtcctaa aatagctttc atgatcgttg
    13381 tttttccagc gccattagga cctactaatc caacgatttt gccaggggat aagctaaaac
    13441 tgacatcgct gagggcttgt ttgtgaccaa agttcttgga taattcttgg accgaaagac
    13501 ttttcaaaaa tattcactcc ttttctatag atagtacaag catacacctg atgagtggag
    13561 aagtcgctag gtcattggac ttatttctta ttttttcata aaaaaattga atattaaata
    13621 aaaaggctta ttttttctga tcagtactta ttccaagtgt ctagtggccg ctagtaatct
    13681 tgatgccaat gatactgatg agtagcatcc ccacaaagaa ccaagtggaa agattcagct
    13741 tatcatgaaa caagaccaca ccaattatga ccgaaccaac tgcgccaatc cctgtccaga
    13801 taggataaga gagactcatc ggcaaacttt tgattgcctt tgacaaaaag taaaaactag
    13861 caatcatgcc aatcactgtg taaaaagaat aattgatttt agaaaaacca tcactccatt
    13921 tcatcatcgt tgaccagaat acttccaaaa ttcctgctat gattaattcc atccatgcca
    13981 tacttacatt cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc atcccttcaa
    14041 agacctaatg gaatgatcat cctctttatc ctacccggtg gcagattgta tatctttttc
    14101 tgtagtatag cacacctatc cagatatcgg caatctcata ctttttagta aagtgtacca
    14161 agcactgacg gatatatcgt tagcggaaaa gaagatgaat gctttgcaat ttcatagaaa
    14221 gaagttgtta tttattaatc atttaaaaaa aacagtaact agaccttatt tctatatatt
    14281 atcgggcaat aaattaaaaa aagctttacc ttacaataag taagataatt tttgtgataa
    14341 aaaaataacg aaaaatattt aaaaatagtg tctatttcgt gagaaaacaa ctatattttt
    14401 tatgtgtttt gctttataat taaaaaaata ttgtcatttt atggaggaaa atatatggaa
    14461 aattatctta gccttttagg cgtcttgatt gttattctcg ggtttgcatt taaattagac
    14521 tcgatattga tcgtaatggt tgctttagtt gttacagctt tgactggcgg tcttggcatt
    14581 caaggaatgt tagaactttt aggtacaagt tttgtaaaca atcgcgggat ggcgattttt
    14641 atcatcatta tgttggcaac aggaacgctt gaaagaaatg gtctgaaaga atcagcggct
    14701 tccttgatca agcgattcaa gaaagtatct gctggtatga ttgtagatat ttatgggatc
    14761 tttcgaatga tctttgcggc ttttaacgtg agttttggtg gagttgctgg atttgttcgt
    14821 ccaatcattt taccgatggc attggggacc atcgaatcaa aagggttaga agtccatcct
    14881 aagcatgaag aagaactaaa aggtatggct tcagctatgg aaaatgtctg ctggttcttt
    14941 ggccaagtac tattcatcgg aggagcaggg gcgttgctag tccaatcaac actgaaagat
    15001 ctaggatatg acgtaacatt gggacagctt gctttagtgg aagttccagt ggctatcacg
    15061 gctttagttg tggcaagtgt ctacttctat cttaaagaaa agaaattagc gaagaaatat
    15121 tatccaggaa aggggcaata agatggactt ttttacaaat cctgatattt tattaggcga
    15181 taaattactg gagatccttt atatcgtgat gggactcgta ttgatttaca ctggagtaag
    15241 aaatttaaca gataaaacca atcctcatcg ttatgggtca gcctttttct ggacggtctt
    15301 aggggtagtt attgcaggag gaagatggct tccggcaatc gtgaacggtg gcttgatctt
    15361 tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa ggagaaagcc ggcttccatc
    15421 aaaagaatat atggaaaaaa tgtcagacaa actaggaatg aaaattttta ttccagcttt
    15481 gagtatcggt gtctttgcga tcttgtttgc tctatttacc aatttaggtg ctttagtcgg
    15541 cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg ttcttttctc gagataatcg
    15601 accaagtaca tttttagatg atgcagcaga tatgctgggc acagtagggc cgttaagcat
    15661 gttgccaatg cttttagctt ctctaggtgc ggtatttaca agcgctggag ttgggacagt
    15721 gatttccaat gcagtaggta cgatcgttcc agaaggaaat gtgaacatcg gcatcatcat
    15781 ttacgctctt ggcatggtga tatttacggt aatcatgggg aatgcatttg cggccatcac
    15841 cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt catggagcga atccagcgtt
    15901 aataggaatg gttgcattga cttgcggttt ttgtgggacc ctgctgacac cgatggctgc
    15961 taacttcaat atcgtgcctg tagcgatgct ggagatgaaa gacaaatacg gagtgatcaa
    16021 aaaccagttg tttattgcgt tatttatgct agttttccaa attgcttata tgattctatt
    16081 taaataatag aagagagggg ttgtgacaga ataattgtcg caacctcttc taacattgta
    16141 gtggaacact atttttttat aagaatatat taagtttgat ctgttatgtt atttaaacta
    16201 ttgtttcctt tatttacgct gttcaaggaa aatggtatga taaaagataa ccatatgtta
    16261 aatcaatcgg acggaggaac aacagatgaa agtactagta acaggttttg atccatttgg
    16321 tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa atgccagatg aaatcagcgg
    16381 tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa aaaagcagtc aagtagtcaa
    16441 agaagccatc caacagcacc aaccagatgt cgtgatctgt gtcggacaag caggtgggcg
    16501 gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca gaagcaagaa tccctgataa
    16561 tgaaggaaat cagccattcg atacggcttt agaagaaaac ggtccagccg cttactttac
    16621 ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat catggtctac cagcgtacat
    16681 ctcttataca gcaggaacat ttgtctgcaa tgatatcatg tatcgtttgc ttcatttgat
    16741 cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac gtgccattct cacccgatca
    16801 agtgatcgat cgtccagtgg gtacgccttc tatgtcttta gaagacattg cttcttcttt
    16861 gacttatgca gttgaagcag cagtagaaaa taaagaagat atttccggaa atgccggaac
    16921 aactcactaa tttagcaaga aacactggct ggttgctaac gttatgttag tagccagctt
    16981 tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg caatccgtct ttgaagaatg
    17041 gtttccaaaa gaaatcagca aaagctatcc catcaaaatt tctaaacaat acacaagcag
    17101 tcaacgttgg gagatctatg caaaattaac gaaaaaacag cgggaactag tcgataagca
    17161 ccgccgatat ctaatcagct ctcgttttat ggaagaacat tatttagctg caacagattg
    17221 ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca aaacgaagcc agcaaaaatt
    17281 gtattgtgag tgtggtagag aattaaaagt gcaatacatc gttaaatcgc caaaaaccgg
    17341 aaagatccta aaactaggca tcaaccactt tgccgatcac ctgcatgtct ctccgacagt
    17401 ggcagcttcg atccatcagg gaatgacaaa agttgatctg gccttagacg aattattatg
    17461 gcttaagcag aagaatatcg attttcctga aggactatgg caaaaatact gttttgtctt
    17521 gtaccaaaat cgtcggatga aacagccata tttaccggat ataaaattag ctcagcggct
    17581 cgcagaattt cgccaagtag aaatgccgat ttacatagcg gattatcaag cattggaaaa
    17641 tgaaatcaag aaaataagtg aacacatcaa tgggcagtca aaaaagcgac agataaaaaa
    17701 agaactattt gacgactttg cagaagaatt agtaaaggat gtagaagaat tcttgatcaa
    17761 ctatcgcgca tttttacgta aagattggca atccatcgta tatgaagaag tgccagtaca
    17821 tcctaatgct tattttgaaa catttatttc cgtgttgaga aagacaaaac gtcaacgaac
    17881 accagaagtc actgctcaga tggaatattt tgcgaaaaac caacgattca tccaaccaaa
    17941 aatctatcta tttatttgga agcaatattg tcactatggt tttacagaag ggttcttcga
    18001 cagcattcct cggatagtca gaaatggttt tttaaaagta ttgcgtaaag aacgagaagc
    18061 tatacaatct gctgataaga aagaccgtac agtctcaaaa gaaaaatggc aactagtagt
    18121 gaaagatatc caatctggaa atgtgcaaga gacaatcgac aaatggaaag gaaaacatta
    18181 tcgttttact gaagctcaga aacaagcgtt ggaatattat cagaagttag aagaatcact
    18241 gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta ctataaaaaa tagaggctgg
    18301 gacagaagcg tttaactcca agaaataaga a
//
LOCUS       DAFBQW010000218        16775 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.guided.058, whole genome shotgun sequence.
ACCESSION   DAFBQW010000218 DAFBQW010000000
VERSION     DAFBQW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.guided.058"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            366..1229
                     /locus_tag="LK889_003195"
     CDS             366..1229
                     /locus_tag="LK889_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK5097329.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1329..1811
                     /locus_tag="LK889_003196"
     CDS             1329..1811
                     /locus_tag="LK889_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK5097330.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2074..2541)
                     /locus_tag="LK889_003197"
     CDS             complement(2074..2541)
                     /locus_tag="LK889_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK5097331.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2664..3515
                     /locus_tag="LK889_003198"
     CDS             2664..3515
                     /locus_tag="LK889_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5097332.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3766..5724)
                     /locus_tag="LK889_003199"
     CDS             complement(3766..5724)
                     /locus_tag="LK889_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5097333.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5721..6593)
                     /locus_tag="LK889_003200"
     CDS             complement(5721..6593)
                     /locus_tag="LK889_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5097334.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(6935..7807)
                     /locus_tag="LK889_003201"
     CDS             complement(6935..7807)
                     /locus_tag="LK889_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5097335.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            7935..8504
                     /locus_tag="LK889_003202"
     CDS             7935..8504
                     /locus_tag="LK889_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK5097336.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8571..9308
                     /locus_tag="LK889_003203"
     CDS             8571..9308
                     /locus_tag="LK889_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5097337.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9539..10807
                     /locus_tag="LK889_003204"
     CDS             9539..10807
                     /locus_tag="LK889_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK5097338.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11294..11758
                     /locus_tag="LK889_003205"
     CDS             11294..11758
                     /locus_tag="LK889_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5097339.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11742..12371
                     /locus_tag="LK889_003206"
     CDS             11742..12371
                     /locus_tag="LK889_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5097340.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12725..14203
                     /gene="msr(C)"
                     /locus_tag="LK889_003207"
     CDS             12725..14203
                     /gene="msr(C)"
                     /locus_tag="LK889_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK5097341.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14250..14696
                     /locus_tag="LK889_003208"
     CDS             14250..14696
                     /locus_tag="LK889_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK5097342.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14895..15410)
                     /locus_tag="LK889_003209"
     CDS             complement(14895..15410)
                     /locus_tag="LK889_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5097343.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15527..16729
                     /locus_tag="LK889_003210"
     CDS             15527..16729
                     /locus_tag="LK889_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5097344.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         5300 a         2989 c         3482 g         5004 t
ORIGIN      
        1 gatgaggttg aacttttaca caagattgtg gacttgactg caaagaaaaa tccaagcagc
       61 gttctgatca gcagtttgaa caacaatttg ctttgtacag taggtagtaa gcggattaac
      121 taagggaata aaagatattt ttaccgcttt gtttacttag aatctgtctt ccccaaaatt
      181 tatttttcta aaggtaaaac atagataggc agataatacc aattatccgt tacgaaacgc
      241 tgcttgagaa ttttctttag atccatacaa cattttctac atctgactaa attggttcct
      301 gattcatcaa gtaattgact ttctttgata atatgataaa aaggcaatca aggaggaaga
      361 taaatatgct taaagatctg aaccaattga tcgattacat cgaagaacat ttgacagaag
      421 gactttccta taaaataata gccgaaaata caggaatctc tgaatatcaa ttaaaacgca
      481 tctttacgtt tattgcaggg atgtcgctaa tggactatat caaaaaccgt agattggctt
      541 tggctaatcg cgatcttcta gatggatcaa gagtgacaga aacggcgttt agatatggat
      601 atgaaacagt cgaaggtttt tcaagagctt ttcgtgattg gtcaggatat ctgccttcag
      661 aaatcagcag atacggcata caaaaatctt ttccacgctt gacatttttt attgatgtca
      721 gaggaggaaa atcgatggaa tttcgtatcg aaaagaaaga acagttttat gtggcaggtg
      781 tggcgaaaaa agttcctgtc aaatttgaag gagtcaatca agaaatacaa aagttagcgc
      841 agacgatcac ggagactcaa agggaagaga tgcataagtt aggagattta tatccaaatc
      901 aagtaatcaa cgtttcttat ggatttgatg gagaccgctt ggaagaaaag ggcagtttga
      961 tgcacatgat tggctttgct acgacaaaag aaaatccgta cgctgattta gaaatgcttg
     1021 ctatagaaga acacacttgg gccatttttc ctaataaagg gccatttccc caaactttac
     1081 aagatacttg ggggaaaatc tatgcagaat ggcttccttc ttcagattat gggttagtcg
     1141 aagctcccga gatctctttt acaaaatggg gagaggattt cagtaatgtt tacagtgaga
     1201 tttggattgc agtgaaagca aaaaaatagt taaaaaataa aaatatccct agaaaaaaga
     1261 gcgtaatcgt gtcatttccc tttaatttta tgtaagatga acttaatata atagaaaggg
     1321 tgggagaaat ggcaaagtat aggatcgaag aaaaaccaga ttttacggtg acgggtattg
     1381 gtgtggaatt gaagagtagc aatacggatt ttgtcggttt ggctgcagag aaacaagcat
     1441 ttgagcaaaa agttatcggt aatggaacaa tagatgcatt gaaacaagct gctgaaaatc
     1501 attatttctt tacagtcaac gaaccctata acgggaaaat gatgtactat gtcggtgttc
     1561 aaacgagtca ggatgttcca aacactacgc gaatcatccc attcccaaaa ggaacgtatc
     1621 ttgttgtaag cgctttagca gaatcgcctg aagaattaag aaatgatttg acgaatacgg
     1681 cctttgggca tgttttaaga gaagtagaag attacgccta tgttgggggt ccaaatgctg
     1741 tagtcgaaat gggagttacc aacgggaaat atatgggaga aatctggata ccggtagtca
     1801 ataaaccatg ataatataga ggtcgtgaaa gtagcgcttt gacctgagca ttagagagga
     1861 aaaatgggaa atagctttcc atattttgca tttttcagaa ctaatgccga gggtcagcta
     1921 cttgaacacc gtttatcaag acgtcatgac ttcagcgctt tgacctgagc attagagagg
     1981 aaaaaggggc atgacaaggc ttttgtcccc cggagaataa aagaatttat gtagacgatt
     2041 ccgatcagaa aaatgcaggc acagtagaga gaattataaa aatctctcta cgaggcctgt
     2101 gttttctggt cttttttctt tttccaaaac ggctatttta tgtatcagac tatcaagtgt
     2161 gatcttagcc atttcttctt ctgccttttt ctgcagccga gcgtagcttt cttctaaagc
     2221 ttcttggata ttcgcaccaa cgacgcaatt tggatttgtt tttggatcga cttggataag
     2281 ctgtgtatta ccttcgatgc tgcgatagac atctagtaaa gaaatctgat ctggactttt
     2341 ggcaagggaa ggctgcggtt ttccggtttt tgtaaagatc aaaccggatt ttttgagctg
     2401 actcatgatt ttacgtacat ttgctgcatt tgtttcgata ctactggcaa tcatctcact
     2461 agaaagtgat cgggtatctt gaaacatttc aatataggca agtacatgga ttgcatcact
     2521 aaattgcaga gaatacttca tcagttgtca tcctttcatt ttttttgctt gacttatctt
     2581 accacactct ttatgataaa ggtgtaattt ataaatgtac agctgtaaat cttaacaaat
     2641 aaaaagaata gggagtggca aatatgcatt atttagtaac aggagcaact ggaggttttg
     2701 gtggttatgc gctggaattc ttaaaaaaga tggttccaca aaatgaaatc tatgtactag
     2761 tacgtagtga agaaaaagga gaagcactga aagaagctgg atttaatata cgtattggtg
     2821 attatgatga tttagactca atgaaagaag cgttgaaagg aatagatcga ttgctttttg
     2881 tctctggcgt tccaggaaat cgtcaagccg aacataagaa tgtagtgaag gcagcacaag
     2941 cagctggggt atcttatatc gcttatacaa gttttgcaga tgccgatcac tcgatcagtg
     3001 tattagcgcc agatcaccaa ttcactgaaa aaatgattaa ggattcaggt atttcccata
     3061 catttttacg gaataattgg tatgtagaga atgaattacc tatcattggg caggctttaa
     3121 aaactggtca attcgtttat gctgcaggaa atggaaaaac aggttgggca ttgaaacgtg
     3181 aatatgcaga agctgcagca aaagtactag tagaaagtga ctctccagaa atcttagaat
     3241 tatccggcaa tctaactgac tatgaagaat tagccaaggc attagagcgt gcgacaggta
     3301 aagagttaga aattattgaa gcaagtgatg cagcgtttgt cgaaaatcta aaagaagccg
     3361 gattccctca agaagcagct gacatgtttt tagcgatcca acatgatatt aaaaaggatc
     3421 agttagctat ttcctctgat gatttagaaa aagtattggg aaaaccatta acttctcttg
     3481 aagcagcatt aaaagaaata ttggatcagg aataatgtat aaagaggttg tgaaaaagcc
     3541 gattagtttt gagtatgaag aaaaattttg tgaaaaagct gttctgcatc aagtaataag
     3601 aagagtcaaa taaaaaatag ctcttcatat gtttcgttta gctggactta ttaccgcgga
     3661 gttggcagga gaacgccgtt tttattcgga aaaaggagct gagacaagat ttttgtctca
     3721 gctcctgttt gtttatcgat aaaaaagtct ggggaagacg atggctcagg ctttgttgga
     3781 aacagagccg attttatcat ttgcttttac atcacgtgcg ggttcgattt ctaagctttg
     3841 gactttgtcg ccatttgtga atacaacgac catatccgtg tttttgcctg cagattgaat
     3901 agcggcaaga tcaactaaag caatcaattg acctcttgcg actttttgac cttcttctac
     3961 atataaagtg aatggttcgc cttttaagtc aacagtgttg atgcccatat gaagcaagac
     4021 ttcgatacca gcatctgttt ggattccgac agcatgtttc gttgggaaaa tgtttgtgat
     4081 cgtaccagcg attggagaga agatttctcc attttctggt aatacagcat agccgtcacc
     4141 catcattttt tccgcgaata catcatctga aacttcagtg attggaatca gtttcccatt
     4201 tgcgatagcg taaatatctt gttcgatcgt ttcaccggca aatgtttctg ttttaaccac
     4261 ggtgtttgtt tgtgcaggtc gtgctgcagc accaccatgt agttttgcca tctcatctgc
     4321 aacgaattgt acttctgtac ctacgatgac ttgcgcattt tttccgtcga tgatcttcat
     4381 accaggtacg ccagtggctt tgattttatt ttgatcgatc aggcctgtat ctttgacttg
     4441 caaacgaagg cgtgtagtac agttatcgat cgaagttaca ttgtcggctc cgcctaatgc
     4501 agcgtagatt ttgcttgctt gtgccgcata tttattgtct gaattagtcg ttgcagtatc
     4561 gtcatctgaa agatctgctt cctcgcggcc aggagtcatc agatggaatt ttttgatagc
     4621 aaagttaaag ccaaagtagt aaatcactgc tgttactaat ccttggacga tcaacatcaa
     4681 tggctgattc gcaatcggat ttttcaaaga taggaagtaa tcgacaaatc cggcactaaa
     4741 ggcaaatcct gctgtccaat ggaagaatgc actcactgct aaagaaatcc cagtgaaaat
     4801 tgcatgcaag acatataatg gccaagcaac gaacataaat gaaaattcaa gtggttctgt
     4861 cacaccagta aagaatgaag caaaagctgc tgctagcatc aaggaagctg tttttgcttt
     4921 gttttctgga cgtgcatttc tgtaaatagc atatgctcct gctggaagcc cgaacatcat
     4981 gattgggaaa aatccagctt gatacattcc tgttacgcct tttgttcctt cagaagccaa
     5041 aaatttacca atatcattga ttcctgctac gtcaaaccag aacacggagt tcattgcttg
     5101 atgcaatcct gtagggatca gtaaacggtt gaagaagcca taaagacctg caccacccca
     5161 gcctaaactc aaaatgaatt tagcaaaaga aactaaagca gagtaaaccg gaggccatac
     5221 gagcagtagc actgcactga tcagcaacat gacaaaggca gccaagattg gcactagacg
     5281 cttgccgcta aagaaagata aagccatcgg taattttgct tcgtggaaac gattataaag
     5341 agctgccgca atcaatccgg caataatacc aataaatacg ttactgatct gagcaaaaga
     5401 tggatcaact gattctggtt tgatattcaa taaagtagct actgaatcag tttttaaaac
     5461 atttactgga acttcaaatg cgaccaaacc tgccaaggca gcagcacctg atttgtccac
     5521 tgacatccct agtgctaacc caacggcaaa cagcaaaggt aattgaccta aaactgcgtt
     5581 accgccagct tgtaaaaaat gagcgacaat gtcgttacta taagatgccc accagtttcc
     5641 gatccctact agtaaagcag cagcaggtaa gaccgcaact ggaagttgaa gggaacgtcc
     5701 cattcgttgt agatatgctt tcaatttttt tcctcctcat gattgttaac atttgtttgt
     5761 acgattgcgc ttcttaaacg ctcgatatgg atggccaagt aacccagttc ttctttcgtg
     5821 atcgcgagct ggtattcttt ggtcagcaac acacggattt ttttagaaat cttatagctt
     5881 tctgggtatt ttcgtttgac catttctacg atttccgtgt ctaaaacagc atattgttgc
     5941 gcatgggttc gttgtagaag tagtcgtaaa tgattcacca gtcttgaata attcagtgaa
     6001 aatggttctg aatggatatc tgtttccaat tctgattcca ctaaatggat gatgtcagat
     6061 acaatcgtca cttcacggat actgcgatgg ttattgttcc gaccgcttcg tccgctgtga
     6121 atatggattg cgatatatcc tgcttcgtca taagtatatg gaatcccaag ctcttttgtc
     6181 agatactcta ctgcccattg agcaatactg aattcatcac tgtaaagcac ttcgatttct
     6241 cttaaaagtt tgttgcggat gacgataccg ttattaatgt tctccgcaga aaaagcaatg
     6301 tggtcggtca aagcgatcaa tacatgttca ttcaactttt ccatcaaaac agtttccgca
     6361 tgatcaatga tcgtttctgc tgcaaataaa aagcgttcat caatctgatt aagtaaattt
     6421 tgcaacttga tttgaccttc tggttccatg acaaatatcc gttcgacatc acgatcaaaa
     6481 atcaaatcat ttctttttct gtcaaatcca atcccttttc caatagctac tttttcctgt
     6541 ccaccgtcat ctaccaatac tgcattttga ttaaagatct ttttgatttt catgcgcgca
     6601 ccctttttga agatttggtt ttcacgtttg ctagaccagt tgattgcggc caaacaaaaa
     6661 tagacacgaa aaacctccta gggaaaccat gttcctcaga gatttttcat gcctagtatg
     6721 atctagtcac atgaaacgaa ttattgttaa cgtaatcaat tttatataga cagttatgtt
     6781 ttgtcaattc tttttttata attatagtaa tttactggaa ttatcagaaa taaaaggaag
     6841 aaataaatta agaaacttat gttatttaat cgttggatct tttgcttatc ttattcgttg
     6901 ttaaaaagct gaaagcgaag gaaaaaaact ctacttaact gtatttctcc gttaggacat
     6961 ctaaaaattt acgagcagca ggagaaagag gctggtcttt tttccaaatg agattcagtg
     7021 aagcttgtaa tggcggatcg agtgggatga atattttttc cgtcccataa gtattgatga
     7081 tcccatctag agctaatgca ccaaaagtcc cgttatcgac catcaaagaa gcattgtaca
     7141 ataagttata tgttccgacg atcgataact gatctatgct gaaacctagc cattcagcta
     7201 gttgactgtc cactgacgtt tgccgggaaa tgatcaaagg aacagttttc agttcctgcg
     7261 gatggatgac ggtttgcttt gctaaaggat gatttcgatg tatcaaaagt ccccaacgat
     7321 cttgggctgg cagtcgaaat gactggtatt tttgtttgtt tgtcggttcg attacgagtc
     7381 caaaatcaag taatccgcta tccagtttgt ctatgatatc atctgcattc ccgctatata
     7441 aatgaaactg gatatcagga tgcgccttga tcaacgaatg aaggctatca gaaatgaaag
     7501 agattgcttt agtttcgcct ccgccaatgt agatttctcc agagatgatt gcgtcatttg
     7561 ttaaattgtt cgccgttttt tctaccaacg acaagatttc ttttcctttt gaataaagat
     7621 atcgaccaga atccgtcaac tgtatctctc gggagccccg agtgaaaagt gtcacaccaa
     7681 gttcttcttc taaatctttc agttgttttg agagagtagg ttgcgtgata tgcagagatt
     7741 ttgctgctcg gctgatattt ttttctctag ctactgctaa aaaatacgtc agtatacgaa
     7801 tatccatttt ttccctcctg tttttaaaaa agcataccac taagtatagt taaaaggaat
     7861 agttaagtat gttttataag tattagacat gataagccgt ccgttataaa gtaaggataa
     7921 aaggagggag caaaatgaca agcctcatcg aaaaacaaat cgtcaataaa gagattcgag
     7981 taacggatcc gctctttgaa gtgatccatc agatccaagc tgaaaacgaa gagcctttag
     8041 cagcgctgaa tactgggtac catgaaccag cagatatccg caaacggcta gaaaatatca
     8101 ttagtgacaa agtagatgat acggtgactg ttctgctgcc tttttataca gatttcggca
     8161 aacatattag tatagggaaa aatgtcttta tcaatcgtca agtgatgttc gttgacttag
     8221 gtggcatctg tttagaagat tcagtattga ttggtcctcg tgtcaacctc atcacggtga
     8281 accatctaac cgatccaaaa gaacgaagag gcttgtcagt aaagccaatc cacatcaaga
     8341 aaaatgcttg gatcggagca ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg
     8401 ccattgttgc agcgaatgca actgttacga aagatgtccc agacaatacc atcgttgcag
     8461 gaatccctgc caaaatcgtc aaacctgtcg aacggattat ttgaacagta agatgaaata
     8521 agagatagtg agtataagca agagattttt caaaacagga ggagaataga atgaatgatt
     8581 tatcagaaaa agtgattgtc atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga
     8641 agctattagc agaaaaaggg gctaaactag ttatagctgc acgccgagaa gatcgcctaa
     8701 aagcaataaa agaatcttta ccagaagctg agctatatat tcaaacagca gatgtaagag
     8761 attttgcaca agtccaagca gttatcgatt tagcgatgga aaagttcggt cgaatcgatg
     8821 tattgtacaa taatgcagga atcatgccaa ccgctccatt agttgaaggt caccgtgatg
     8881 aatggcaaaa tatgcttgat atcaacatca tgggtgtgtt aaatggaatt tccgccgtac
     8941 ttccaatcat ggaaaaacaa aaatctgggc atatcatttc aactgattct gtggcgggac
     9001 atgttgttta tcctgattcc gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca
     9061 tggaaggatt acgtcaagag caacggcaaa ataacatcaa atctacgatc atctctccag
     9121 gagctgttca aactgaatta tatcaaacca tcagcgacaa aaaggctgct cttgaacttc
     9181 atgaagcgca aaaagaatgg ggattgacct ccgaagatat agcttctgct gtagcatttg
     9241 cgatagaaac gccagaccgg atgtctgtca gtaacatgat tatccgtccg acgacacagg
     9301 aagtataatc cataacggat aatatccaaa gagaattact tgttaaatag ttattcaaac
     9361 gaccatcaat ttaaggcgtg aatggagaag aatattcttc atttacgtct ttttatgtag
     9421 aaaaagcaaa ttcatcaaaa agaaacaaat gttatttaaa tatggacatt gacttgcttt
     9481 tactttttta gttacactgt tatttaggaa aagtttcata attcaaagga gaaaaaagat
     9541 gaaagcaaag aatttattat ttagcgttat tcttttagga aatctagcag ttgccaacac
     9601 aacagtgcta gctgaagaat cgacagaaac tgtcgcaact cagacatcga gttcttcaga
     9661 aacaggaatt gaaaacgata cagagagtac tttgcagtca gctactgtct cagaaaatac
     9721 aatttcaacc gaagaaccaa caactgaaac aacagaacaa acacaaaccg aagacagtac
     9781 atcagcagaa gctcagaaca atccagagaa tgcagaagaa atcacgaacc aattgacaga
     9841 tgggcaaaca ttgacacata cccatcaagc agatttgtct gaattcgaaa actatactgg
     9901 ctatgctttt tatgctcgct catctgctaa ttctcaacaa gcgttcattg atagtattgc
     9961 ctcaactgca caaagtttgg caagtgccaa tgacttatat gcatcagtca tgattgctca
    10021 agccatcgtt gaaagtggct gggggaacag tactttggct tctgccccta attacaatct
    10081 atttgggatt aaaggaagct ataatggcca atctgttacg atgcctactt cggaatatgt
    10141 gaatgggcaa tggattacgg tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc
    10201 attgcaagat aacgtaacgg tattgaagac gacttctttt cagccaggcg tttactacta
    10261 ttccggtgca tggaaaagca atacgaactc ttacaaagat gctacggctt ggctaacagg
    10321 tcgttatgcc actgcgccta attatggttc tacattgaac aatgtaatcg aaacctataa
    10381 tctgacacag tatgatacag caccaacttc aacttctccg atgtatcgtc tgtataaccg
    10441 tcatacagga gaacatttat atacattgaa tgccggagaa aaaaattatc tgccaactgt
    10501 aggctgggaa tatgaaggga ttgcttggca agcaccaaac agcggacaac ctgtctaccg
    10561 tttatacaat ccttacagtg gagaccatca ttacacaatg gcacaatctg agatcaactc
    10621 tttaacaaaa atcggttggc gttatgaagg tctaagtttt tattctggtg gttccaagcc
    10681 aatttatcga ctatttaatc caaatgaaaa aactggtacc catcactata ccttgagtgc
    10741 taaggaaaga gattggctaa caccgatggg ctggagatat gaaggaatag gattttatgg
    10801 ttattaaaaa gaggtagtga aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa
    10861 aaacagattt tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa
    10921 caccgtttac tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta
    10981 agcatactga tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat
    11041 ttttggcggt gtaaaatttt ctactgcaga agagctatct tctatagttt gataagaatc
    11101 aataatcttg gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa
    11161 atttcttttc tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt
    11221 gacttttttc tttttttatt ctattattga caagtcaatc gttgacgagt caattattag
    11281 aaaggaggaa attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc
    11341 cgatcaaaaa atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga
    11401 gatcatgatg agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt
    11461 gcagattgat caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt
    11521 ttcgcgtcag agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc
    11581 ccagaagaaa atcgagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc
    11641 ggctttcagc gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca
    11701 actttgcaat ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt
    11761 tttcaacaat tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc
    11821 cacacgaaga aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata
    11881 tgcaaccatg gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt
    11941 tgatccgttt caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg
    12001 atatgcaatg ttacgaatat ggcgaagaaa tctataacca agcttacgaa tcagggaaga
    12061 tgccaaaaga agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt
    12121 caagagaaga aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca
    12181 tgttagttgc gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc
    12241 agttggcaga ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa
    12301 taggaaaagc ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta
    12361 cgttcgaata aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg
    12421 tggtaaccta ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga
    12481 acttttgaat aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg
    12541 gagtatgtcc attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga
    12601 tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca
    12661 gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa
    12721 acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct
    12781 agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga
    12841 aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca
    12901 tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga
    12961 agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc
    13021 agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc
    13081 ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga
    13141 tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg
    13201 ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga
    13261 gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct
    13321 acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa
    13381 gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag
    13441 accggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa
    13501 tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat
    13561 gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat
    13621 gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca
    13681 gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt
    13741 attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca
    13801 agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat
    13861 gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc
    13921 tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac
    13981 gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt
    14041 tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat
    14101 tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat
    14161 aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt
    14221 tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt
    14281 ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc
    14341 atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt
    14401 tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag
    14461 taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa
    14521 tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat
    14581 tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga
    14641 tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga
    14701 acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt
    14761 ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt
    14821 tccaactgta ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag
    14881 gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat
    14941 tggagttcct gctgacaaag cataattctg cgctggatca caacataacg tccaaattct
    15001 tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc
    15061 agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa
    15121 gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc
    15181 cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc
    15241 tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag
    15301 atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa
    15361 cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta
    15421 cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata
    15481 tttacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga
    15541 gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct
    15601 agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt
    15661 aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag
    15721 ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg
    15781 tttgaaaaaa acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg
    15841 attatctggg aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc
    15901 tttatttgtc tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa
    15961 agcaatcatg atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg
    16021 tggggtcttg ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt
    16081 ttgcggtttt atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt
    16141 aggcctttgg gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat
    16201 cgcattgtta tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat
    16261 tggctattct gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat
    16321 tgcttcgatt ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt
    16381 agcagcattt gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc
    16441 agtccttgta ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact
    16501 gttgacgacg attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta
    16561 tagttttgtc cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga
    16621 acatattggt gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg
    16681 ttgtatcatt tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg
    16741 ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAFBQW010000219         8062 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.guided.103, whole genome shotgun sequence.
ACCESSION   DAFBQW010000219 DAFBQW010000000
VERSION     DAFBQW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.guided.103"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            128..1564
                     /locus_tag="LK889_003211"
     CDS             128..1564
                     /locus_tag="LK889_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5097345.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="LK889_003212"
     CDS             1753..4200
                     /locus_tag="LK889_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK5097346.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="LK889_003213"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="LK889_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK5097347.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="LK889_003214"
     CDS             complement(5690..6793)
                     /locus_tag="LK889_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5097348.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="LK889_003215"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="LK889_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK5097349.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAFBQW010000220         7786 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.guided.107, whole genome shotgun sequence.
ACCESSION   DAFBQW010000220 DAFBQW010000000
VERSION     DAFBQW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.guided.107"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            245..1213
                     /locus_tag="LK889_003216"
     CDS             245..1213
                     /locus_tag="LK889_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK5097350.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="LK889_003217"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="LK889_003217"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK5097351.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="LK889_003218"
     CDS             2243..2851
                     /locus_tag="LK889_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK5097352.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4196
                     /gene="vanY"
                     /locus_tag="LK889_003219"
     CDS             3279..4196
                     /gene="vanY"
                     /locus_tag="LK889_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBK5097353.1"
                     /translation="MKKLFFLLLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKE
                     HLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYG
                     LLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPA
                     GYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWH
                     IRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPT
                     NLRYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4349..4834
                     /gene="vanZ"
                     /locus_tag="LK889_003220"
     CDS             4349..4834
                     /gene="vanZ"
                     /locus_tag="LK889_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK5097354.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5121..5450)
                     /locus_tag="LK889_003221"
     CDS             complement(5121..5450)
                     /locus_tag="LK889_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5097355.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5755..6084
                     /locus_tag="LK889_003222"
     CDS             5755..6084
                     /locus_tag="LK889_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5097356.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6074..6361
                     /locus_tag="LK889_003223"
     CDS             6074..6361
                     /locus_tag="LK889_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5097357.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6640..7203)
                     /locus_tag="LK889_003224"
     CDS             complement(6640..7203)
                     /locus_tag="LK889_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5097358.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2567 a         1297 c         1547 g         2375 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt
     3361 tttctcagga aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa
     3421 atagtgggac ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag
     3481 gaaatctgct attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata
     3541 tcgtgaattt atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata
     3601 tttatatgtc aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg
     3661 gtggcgttag tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc
     3721 tttaccaaga aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag
     3781 gtttatcact agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt
     3841 ggatagaaga aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag
     3901 agttaacagg aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg
     3961 cgattatgaa agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa
     4021 aaaccatttc tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta
     4081 aaaataccac cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag
     4141 acggtgtaat tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat
     4201 ggcggaatga aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa
     4261 cgttagcttt ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac
     4321 ttaaacaaaa gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc
     4381 tttatattta gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc
     4441 agtatttaat tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt
     4501 cagagagatg atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa
     4561 ttttaaagaa atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt
     4621 tgaaataatt caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa
     4681 tactgttgga ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca
     4741 aaaaaaatta gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt
     4801 ttaccgtacc catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct
     4861 ttcatacaca taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac
     4921 tcgcccgtag gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac
     4981 tatagtaaca aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata
     5041 aatttcctta acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat
     5101 agatagattt tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct
     5161 aaagagtaat aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa
     5221 aaacgtaaat ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc taaatcacag
     5281 acgcacattt ccttataggt ttctaaagca tagaaaattt taattctaga aaaatcgctg
     5341 aaacatttcc ctaacacaag taagtttgaa aaatcttttt gatccaattt attttttatt
     5401 ccatttactt tttctttatg tacgacagtc acttggcaaa cttttttcat tcatctcacc
     5461 tcacattcaa acgattattt gaatgaagta tacctatttg atcactcttt tgtcaatcta
     5521 ccttaattcc aatacactat tataagcaat ttcatgtatt gaagtaaact tacttgcaag
     5581 tgcgtttttg attccaagtt aaaaacatat tattattcta cataatttcc taatcaaata
     5641 aaactccttt gaaaattgtt ctaaaggagt tttattattt tgtatttcta ctaaaaatag
     5701 cgtacaatgt aattactaag taattacgaa atgtaaaata atggaggaaa taatatgatt
     5761 acaaaaacaa gaaaacaagg aaattctatt atgttaacgg tccctaaaga attcgatgtg
     5821 cccaatggtg ttgaagtaga agctaagctt gtagagaatg gtatccttta tgagtttgta
     5881 gagcctaaaa aagaattttt tgattttagt gaagatattt tggctgatat tctttcagaa
     5941 ggttatagca aacaagagat cttaaaagaa tttagaaata gaaaaaatgc gttaacttct
     6001 gcatttaaat ctattgctga agatactgtt gcaaattcta aaccgatgac aaaagaggag
     6061 ttggcagcag aaattggctt atgagattct accttctaaa cacgtaataa agtatctaaa
     6121 aaaattaaaa gagaaaacat taaaagaaca atttttaacg ataatttatg atgagatagc
     6181 agttcgtcct cattcgggag aacaaaaaac gggtgattta tcaggaattt gggctattgg
     6241 attcaagtat gcaggtacga catatcgcgt tgcatatgaa attaaagata atgcagtgat
     6301 acctatctta ctatgtggaa cgcatgaaaa tttttatgaa caattaaaaa agattaggta
     6361 agaacccaat gtttcaactt gagaggatag acgacacttt aaaagataat ttttaacagc
     6421 tgagagttgt aataccctat aattttaaac cactaaccta tatatttatg aaaaatagaa
     6481 aaaattaaaa cgcattttaa gcctctttca aattaaaagc ttcatcctta tgcacaaaga
     6541 gggcttaaaa tgcgttattt tatattgttt tttagtaata ataaaaaaat cactctaatt
     6601 ctcccataaa tattatagat tcctaaagat taaaatagat tattccctat tgtcttttaa
     6661 gtagcggtag agtgttgcct tgctaatacc agtcatttcc gtaatttctt ttacgctata
     6721 aacctgactt ttatacaatt ttactgctcg atcaacatct ttctggttca ctcgtggacg
     6781 gccaccttta cgacctctcg cacgtgcact tttcaaacct tctttggtac gttcaacaat
     6841 taaatcacgt tcgaactgac taaatgcttg gaagacagtc atcattaagc gtccgtgagg
     6901 cgtgctggta tcgaagtttt ctttcaagct caccagctta acatggtgtt cttcaaaata
     6961 ggtaacaagg tcaatcaaat cctttgtact acgacccaat cgagaaaaac tttctacaat
     7021 taccgtatct cccggacgaa ttttatcttt taaacgattt agttggggac gaaccgcttt
     7081 ggttcccgtc attttctcag ttaggatttc attacaattt tgttcattga gtaagtctaa
     7141 ttgacgattt agttcttgtg aacgtgtact cactcgggca taaccataaa tgtatccact
     7201 catgcttttc ttcctttcgt actttaattc tggcttgatt gtatcataaa agggagttat
     7261 agatacatag ttaatgcaac aagttatgat acaaaatatg acctctttta tttaataaaa
     7321 gaggtcattt cattttgtct cataatcgat tgtttttgat acgagtgtgt atacgaaata
     7381 aacaaaaaaa tcttaagtaa atttctagat acctaattta atcctaacta gtgtatccta
     7441 aagttaataa cgagtttatc acaatgaaaa ttataaaatg ctcccatatt agatataaca
     7501 tgcaatattt gaattagaac tacaaaattt attcgtttta taaagtaaat aatgttgaga
     7561 tactaaggaa tagaatatta taaggaattg agctgaatgt attggataaa aaaagaataa
     7621 ctttattaga gatggactat aaaaatgctc gtaattttct tctgaagtct gaaagctatt
     7681 tgaatgctca gctaccagaa tatattagtt ttgacgaaac tttaaaatct gctaaaaaag
     7741 ttttaacttc gccgtttgta taattaagcg agacaaataa aaaagc
//
LOCUS       DAFBQW010000221         5246 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.guided.123, whole genome shotgun sequence.
ACCESSION   DAFBQW010000221 DAFBQW010000000
VERSION     DAFBQW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.guided.123"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            111..194
                     /locus_tag="LK889_003225"
     CDS             111..194
                     /locus_tag="LK889_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBK5097359.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="LK889_003226"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="LK889_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK5097360.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="LK889_003227"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="LK889_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1649..2314
                     /locus_tag="LK889_003228"
                     /pseudo
     CDS             1649..2314
                     /locus_tag="LK889_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2350..2595
                     /locus_tag="LK889_003229"
     CDS             2350..2595
                     /locus_tag="LK889_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5097361.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2698..3567
                     /locus_tag="LK889_003230"
     CDS             2698..3567
                     /locus_tag="LK889_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK5097362.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3548..4282
                     /locus_tag="LK889_003231"
     CDS             3548..4282
                     /locus_tag="LK889_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5097363.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4315..5230
                     /gene="ant(6)"
                     /locus_tag="LK889_003232"
                     /pseudo
     CDS             4315..5230
                     /gene="ant(6)"
                     /locus_tag="LK889_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
BASE COUNT         1871 a          767 c         1036 g         1572 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg tctttccatt tagatagtag tggaaagacc
     1561 tctatttatc ttttgaaagt gaatacaaaa ataaagcaca ctctgctata atataggtga
     1621 ttaatccatt tgagagaggt gctttatcat gtatcaaaat tataccataa accaactttg
     1681 tttgcctata gatcttgaaa ttaaattaga agaaaatgat tttgctcatg ccattgtgca
     1741 gtttgttgac tctattcccg atgaagtctt tcttccttat tatcaatcta tgggtagacc
     1801 ccaatatcat cctcgtatgc tactttccat tattctttgt gcctatattc aaggggttta
     1861 ttcgggccgc caaatacaaa atatgctgat agatagtatt cggatgcgct acttatctca
     1921 agagcaattc cctaactttc gtactattaa tcgttttcgc gttcatccga ttatgaacga
     1981 tattctagat cattcatttg ttcaatttcg tgagctactg gttcaaagtg gcttaatcag
     2041 tggcagtgct ctatacattg atggtacaaa aattgaagcg gatgcgaata aatactcttt
     2101 cgtttggaaa aaaagtattt taaagtataa ggatagcctt gatcaaaaag cccttgaaaa
     2161 ctaccaacaa atggtggaaa caaaaatctt acctgagtta atagatgatt taaaggatga
     2221 actttctata gaagagattg aaaggattcg tcaatctttg gaagaaaagg aaaatcaatt
     2281 aattgaagcg attgaccaaa ccgaaacagt agaagggggc tgacccatta ttaataagga
     2341 gtggacaaca tgattgatgt aaatgagcta ttggaagaag ctattagaga aactgaaaat
     2401 ctaaatgatg gcgaagtttt tcttgttaaa gatttattca agggatatgt ggggaatagg
     2461 atacccagaa aggatcgact tctgcttgga acgttatttt taaaccatgt tagcaaaatg
     2521 aatggtaatt taaaggctat tgaaaagact tcatctaatc agcagaggta taaaaagaca
     2581 attgataagt tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg ttgagtagaa
     2641 gcaaaagatt tggcttggaa tttttacagc agtgaatcga taaaaggagc atgcttgatg
     2701 gtagataata ttattaaatc agtagcagag aaattatcct ctctgtctta tatagaaggt
     2761 attgttttag gtggttcacg tgcaaggggc acccatacag aggattcgga tatagatatc
     2821 ggcatctatt acaattcaga atcatttgac ataaatacta ttaatcaatt cgctacaaag
     2881 ctggatgatg agcatagaaa taaccttgtt gtacctcccg gagcatgggg tgattggatt
     2941 aatggcggcg gatggttagt cataaacggg tatcatgtgg atttaatttt acgtgatatt
     3001 aaacgtgtgg aacaaataat gaaagataca gagcacggaa ttgttactgc caattatcag
     3061 actgggcatc cccatggtta tattagtgca atgtatcgag gagaattagc gattagcaaa
     3121 atactatatg ctaagaatga aagcttatgc gaattaaaaa aacaggcaga aacttatccc
     3181 aatgctttgc agaaaagttt agttaacttt tttatgtttg aagcagggtt ctctttaatg
     3241 tttgtaaaag caaattcggg aacagacgat aaatattata ttgcgggtca tgtttttcgt
     3301 atagtttcat gtttaaatca agtgttattt gcatgtaata atgcttattg tatcaacgaa
     3361 aagaaagcta taaaactgct tgaaactttt gaacataaac ctgaaaaata taccgagaag
     3421 gtaaatcata tttttgaagt actcggtatc tcactttttg aatgctacga catgaccgag
     3481 aagctttata atgaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag
     3541 agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat
     3601 gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct
     3661 acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat
     3721 atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct
     3781 cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga
     3841 agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta
     3901 tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa
     3961 tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta
     4021 taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg
     4081 gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct
     4141 aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga
     4201 gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt
     4261 atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga
     4321 tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga
     4381 attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat
     4441 tatgatatta catattttgt aagtgatata gaacctagaa ccgtttatat ctaatgatga
     4501 ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata tggaattatt
     4561 cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga
     4621 tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa
     4681 ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg atatagatta
     4741 tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt
     4801 aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt
     4861 taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac
     4921 aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga
     4981 tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc
     5041 attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca
     5101 ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa
     5161 atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag aagagagact
     5221 aactatatag agtcaagtaa aaatta
//
LOCUS       DAFBQW010000222         2246 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.guided.161, whole genome shotgun sequence.
ACCESSION   DAFBQW010000222 DAFBQW010000000
VERSION     DAFBQW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.guided.161"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..152
                     /locus_tag="LK889_003233"
     CDS             <1..152
                     /locus_tag="LK889_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5097364.1"
                     /translation="YLIVDLKLCNRTNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
     gene            366..1061
                     /gene="vanR"
                     /locus_tag="LK889_003234"
     CDS             366..1061
                     /gene="vanR"
                     /locus_tag="LK889_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK5097365.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1039..2193
                     /locus_tag="LK889_003235"
     CDS             1039..2193
                     /locus_tag="LK889_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK5097366.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          758 a          410 c          475 g          603 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa ttatgcggta aagctatata
       61 aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat
      121 tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata
      181 tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc
      241 cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc
      301 ttataaattc ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga
      361 taactatgag cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg
      421 aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat
      481 tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca
      541 caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc
      601 tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt
      661 atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc
      721 gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc
      781 ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc
      841 ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg
      901 agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca
      961 ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata
     1021 taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa
     1081 ctagaacgaa aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg
     1141 ttgtatattc gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa
     1201 aacaaatatg acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac
     1261 aatatagata tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc
     1321 atgctttcaa aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt
     1381 cagaacgaag ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc
     1441 aacacattaa aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga
     1501 aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc
     1561 ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat
     1621 gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag
     1681 attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat
     1741 atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg
     1801 gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc
     1861 tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt
     1921 accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca
     1981 aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc
     2041 gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga
     2101 gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg
     2161 atgccagact tggttgataa aaggaggtcc taagagatgt atataatttt ggctctttgt
     2221 caaatgatgt tggtaaagag aaatct
//
LOCUS       DAFBQW010000223         1574 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2350
            SAMN22135350-rid13228063.guided.172, whole genome shotgun sequence.
ACCESSION   DAFBQW010000223 DAFBQW010000000
VERSION     DAFBQW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135350
            Sequence Read Archive: SRR16347134
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162979.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:22:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,170
            Genes (coding)                    :: 3,098
            CDSs (with protein)               :: 3,098
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135350-rid13228063.guided.172"
                     /isolate="P-2350"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            49..591
                     /gene="sat4"
                     /locus_tag="LK889_003236"
     CDS             49..591
                     /gene="sat4"
                     /locus_tag="LK889_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBK5097367.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            684..1478
                     /locus_tag="LK889_003237"
                     /note="aph(3')-IIIa"
     CDS             684..1478
                     /locus_tag="LK889_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBK5097368.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          528 a          260 c          379 g          407 t
ORIGIN      
        1 caaaaccgga aggttaaaac cctaactcta atatacaagg cgggaacagt gattacagaa
       61 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
      121 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg
      181 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
      241 gaatatattg acaatactga taagataata tatctttact accaagacga taaatgcgtc
      301 ggaaaagtta aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta
      361 tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg
      421 gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct
      481 tgtaaattct atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc
      541 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
      601 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
      661 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
      721 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
      781 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
      841 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
      901 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
      961 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     1021 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     1081 cgaatagctt agacagccga ttagccgaat tggattactt actgaataac gatctggccg
     1141 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
     1201 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     1261 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     1321 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
     1381 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     1441 aaataaaata ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa
     1501 aagctttaac tacaagtttt ttagacatct aatcttttct ggctctttgt caaatgatgt
     1561 tggtaaagag aaat
//
